← Back to search

Protein Structures and Oligomeric States

Enter a protein name, UniProt accession or PDB ID. Name searches ask you to confirm the protein identity before related structures are queried.

Current Protein Identity：P32639 New Search
18PDB entries
20Biological assembly
20chain/assembly records
Main Difference Dimensions in This Set
Different construct Different mutation/modification Different assembly state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics

Difference tags compare only the current result set; every original PDB and assembly record remains separate.

Related-Structure Differences

Each row represents one biological assembly in one PDB entry; multiple monomers of the same protein are listed separately.

PDB Entry Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Experimental Method Experimental Conditions Structure Quality
3HIB Crystal structure of the second Sec63 domain of yeast Brr2 Deposited 2009-05-19 Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count
Chain A 1851–2163（313 aa） Fragment：Second Sec63 domain: UNP residues 1851-2163
Non-standard monomer：Yes (specific site not provided by mmCIF) No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6；298 K；0.1M Sodium citrate, 17% PEG 8000, 0.2M NaCl, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K
Resolution 2.00 Å R-free 0.257
3IM1 Structure of the C-terminal Sec63 unit of yeast Brr2, P212121 Form Deposited 2009-08-09 Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count
Chain A 1839–2163（325 aa） Fragment：Sec63 unit (UNP residues 1839-2163)
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6；293 K；100mM sodium cacodylate, pH 6.0, 100mM Li2SO4, 15% PEG 4000, VAPOR DIFFUSION, HANGING DROP, temperature 293K
Resolution 1.65 Å R-free 0.232
3IM2 Structure of the C-terminal Sec63 unit of yeast Brr2, P41212 Form Deposited 2009-08-09 Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count
Chain A 1839–2163（325 aa） Fragment：Sec63 unit (UNP residues 1839-2163)
Not recorded PG4 TETRAETHYLENE GLYCOL × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6；293 K；100mM sodium cacodylate, pH 6.0, 100mM Li2SO4, 15 % PEG 4000, VAPOR DIFFUSION, HANGING DROP, temperature 293K
Resolution 1.99 Å R-free 0.237
3JCM Cryo-EM structure of the spliceosomal U4/U6.U5 tri-snRNP Deposited 2015-12-23 Assembly 1 Protein–RNA Heteromer；Protein × 30 PDB declaration: 34-meric（34） Consistent with all polymers
Chain N 1–2163（2163 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 M7M N,N,7-trimethylguanosine 5'-(trihydrogen diphosphate) × 1 ELECTRON MICROSCOPY
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.80 Å
4BGD Crystal structure of Brr2 in complex with the Jab1/MPN domain of Prp8 Deposited 2013-03-25 Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count
Chain A 442–2163（1722 aa） Fragment：RESIDUES 442-2163
Not recorded ADP ADENOSINE-5'-DIPHOSPHATE × 1 MG MAGNESIUM ION × 1 PE5 3,6,9,12,15,18,21,24-OCTAOXAHEXACOSAN-1-OL × 1 X-RAY DIFFRACTION
X-ray crystallization conditions pH 7.5；100 MM HEPESNA PH 7.5, 200 MM MGCL2, 32-40% PEG400
Resolution 3.10 Å R-free 0.270
5DCA Crystal structure of yeast full length Brr2 in complex with Prp8 Jab1 domain Deposited 2015-08-23 Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count
Chain A 113–2163（2051 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；291 K；0.1 M MES-NaOH, pH 6.5, 9 % (w/v) PEG 3350, 0.2 M MgCl2
Resolution 2.80 Å R-free 0.255
5GAN The overall structure of the yeast spliceosomal U4/U6.U5 tri-snRNP at 3.7 Angstrom Deposited 2015-12-15 Assembly 1 Protein–RNA Heteromer；Protein × 32 PDB declaration: 35-meric（35） Consistent with all polymers
Chain B 1–2163（2163 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
Resolution 3.70 Å
5GAO Head region of the yeast spliceosomal U4/U6.U5 tri-snRNP Deposited 2015-12-15 Assembly 1 Protein–RNA Heteromer；Protein × 10 PDB declaration: 11-meric（11） Consistent with all polymers
Chain B 1–2163（2163 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
Resolution 4.20 Å
5GAP Body region of the U4/U6.U5 tri-snRNP Deposited 2015-12-15 Assembly 1 Protein–RNA Heteromer；Protein × 9 PDB declaration: Dodecameric（12） Consistent with all polymers
Chain B 1–2163（2163 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE；Grids were blotted at 4 deg C for 2 seconds before plunging.
Resolution 3.60 Å
5GM6 Cryo-EM structure of the activated spliceosome (Bact complex) at 3.5 angstrom resolution Deposited 2016-07-12 Assembly 1 Protein–RNA Heteromer；Protein × 41 PDB declaration: 46-meric（46） Consistent with all polymers
Chain B 1–2163（2163 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 5 ZN ZINC ION × 13 ADP ADENOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8；CEB buffer (10 mM Tris-HCl, pH 8.0, 75 mM NaCl, 1 mM Mg(OAc)2, 1 mM imidazole, 0.01% NP40, 1 mM TCEP, 0.5 mM EGTA)
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.50 Å
5LJ5 Overall structure of the yeast spliceosome immediately after branching. Deposited 2016-07-17 Assembly 1 Protein–RNA Heteromer；Protein × 40 PDB declaration: 45-meric（45） Consistent with all polymers
Chain B 1–2163（2163 aa）
Not recorded MG MAGNESIUM ION × 2 ZN ZINC ION × 7 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.8
cryo-EM vitrification conditions Cryogen ETHANE；3 microlitres sample were applied to the grid, left for 30 seconds and then blotted for 2.5-3.0 seconds before plunging.
Resolution 10.00 Å
5LQW yeast activated spliceosome Deposited 2016-08-17 Assembly 1 Protein–RNA Heteromer；Protein × 27 PDB declaration: 31-meric（31） Consistent with all polymers
Chain C 1–2163（2163 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.3
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 5.80 Å
5M52 Crystal structure of yeast Brr2 full-lenght in complex with Prp8 Jab1 domain Deposited 2016-10-20 Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count
Chain A 1–2163（2163 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；291.15 K；0.1 M Tris-HCl pH 7.5, 10.5 % (w/v) PEG 3350, 0.2 M MgCl
Resolution 3.40 Å R-free 0.296
5M52 Crystal structure of yeast Brr2 full-lenght in complex with Prp8 Jab1 domain Deposited 2016-10-20 Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count
Chain B 1–2163（2163 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；291.15 K；0.1 M Tris-HCl pH 7.5, 10.5 % (w/v) PEG 3350, 0.2 M MgCl
Resolution 3.40 Å R-free 0.296
5M5P S. cerevisiae spliceosomal helicase Brr2 (271-end) in complex with the Jab/MPN domain of S. cerevisiae Prp8 Deposited 2016-10-22 Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count
Chain A 271–2163（1893 aa） Fragment：UNP residues 271-2163
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.5；291 K；0.1 M MES pH 6.5 9.2% (w/v) PEG 4000 0.4 M MgCl2
Resolution 4.20 Å R-free 0.335
5M5P S. cerevisiae spliceosomal helicase Brr2 (271-end) in complex with the Jab/MPN domain of S. cerevisiae Prp8 Deposited 2016-10-22 Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count
Chain C 271–2163（1893 aa） Fragment：UNP residues 271-2163
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.5；291 K；0.1 M MES pH 6.5 9.2% (w/v) PEG 4000 0.4 M MgCl2
Resolution 4.20 Å R-free 0.335
5NRL Structure of a pre-catalytic spliceosome Deposited 2017-04-24 Assembly 1 Protein–RNA Heteromer；Protein × 53 PDB declaration: 58-meric（58） Consistent with all polymers
Chain B 1–2163（2163 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ZN ZINC ION × 7 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9；Buffer pH: HEPES, 7.9; EDTA, 8.0
cryo-EM vitrification conditions Cryogen ETHANE；Grids were glow-discharged for 15 s before deposition of 3 microliter sample (~1.5 mg mL-1), and subsequently incubated for 2-3.5 s before blotting and vitrification by plunging into liquid ethane with a Vitrobot Mark III (FEI) operated at 4 degrees Celsius and 100% humidity.
Resolution 7.20 Å
5ZWM Cryo-EM structure of the yeast pre-B complex at an average resolution of 3.4~4.6 angstrom (tri-snRNP and U2 snRNP Part) Deposited 2018-05-16 Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 57-meric（57） Consistent with all polymers
Chain D 1–2163（2163 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ZN ZINC ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
5ZWO Cryo-EM structure of the yeast B complex at average resolution of 3.9 angstrom Deposited 2018-05-16 Assembly 1 Protein–RNA Heteromer；Protein × 55 PDB declaration: 60-meric（60） Consistent with all polymers
Chain D 1–2163（2163 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.90 Å
7B9V Yeast C complex spliceosome at 2.8 Angstrom resolution with Prp18/Slu7 bound Deposited 2020-12-14 Assembly 1 Protein–RNA Heteromer；Protein × 45 PDB declaration: 50-meric（50） Consistent with all polymers
Chain B 1–2163（2163 aa）
Not recorded MG MAGNESIUM ION × 6 K POTASSIUM ION × 1 KGN D-chiro inositol hexakisphosphate × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.80 Å

This page presents structural evidence recorded in PDB/mmCIF.“No recorded non-water small molecule” does not prove that the experimental sample was ligand-free; experimental conditions belong to the PDB experiment, while ligands and copy counts are shown for each biological assembly.