Current Protein Identity：Q04062
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Difference tags compare only the current result set; every original PDB and assembly record remains separate.
Related-Structure Differences
Each row represents one biological assembly in one PDB entry; multiple monomers of the same protein are listed separately.
| PDB Entry | Assembly / Oligomeric State | Construct | Mutations and Modifications | Ligands, Ions and Non-polymers | Experimental Method | Experimental Conditions | Structure Quality |
|---|---|---|---|---|---|---|---|
| 2MQW Solution structure of a proteasome related subunit N terminal domain Deposited 2014-06-30 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
1–160（160 aa）
Fragment：N terminal domain, UNP RESIDUES 1-160
|
Not recorded | No recorded non-water small molecule | SOLUTION NMR |
NMR measurement conditions
pH 7；298 K；Ionic strength (raw mmCIF value) 210；Pressure AMBIENT
NMR sample composition
0.5 mM [U-100% 13C; U-100% 15N] Protein-1, 50 mM sodium phosphate-2, 100 mM sodium chloride-3, 90% H2O/10% D2O | 90% H2O/10% D2O
NMR sample composition
0.5 mM [U-100% 15N] Protein-4, 50 mM sodium phosphate-5, 100 mM sodium chloride-6, 90% H2O/10% D2O | 90% H2O/10% D2O
|
Resolution not provided |
| 2MR3 A subunit of 26S proteasome lid complex Deposited 2014-06-30 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
1–393（393 aa）
Fragment：UNP RESIDUES 1-393
|
Not recorded | No recorded non-water small molecule | SOLUTION NMR |
NMR measurement conditions
pH 7；303 K；Ionic strength (raw mmCIF value) 0.21；Pressure ambient
NMR sample composition
0.5 mM [U-100% 13C; U-100% 15N] Protein-1, 100 mM sodium chloride-2, 50 mM sodium phosphate-3, 90% H2O/10% D2O | 90% H2O/10% D2O
NMR sample composition
0.5 mM [U-13C; U-15N; U-2H] Protein-4, 100 mM sodium chloride-5, 50 mM sodium phosphate-6, 90% H2O/10% D2O | 90% H2O/10% D2O
|
Resolution not provided |
| 2MRI Solution structure of a proteasome related subunit C terminal domain Deposited 2014-07-06 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
181–356（176 aa）
Fragment：UNP RESIDUES 181-356
|
Not recorded | No recorded non-water small molecule | SOLUTION NMR |
NMR measurement conditions
pH 7.5；298 K；Ionic strength (raw mmCIF value) 0.07；Pressure ambient
NMR sample composition
0.5mM [U-100% 13C, U-100% 15N] Protein-1, 20mM TRIS-2, 50mM sodium chloride-3, 90% H2O/10% D2O | 90% H2O/10% D2O
|
Resolution not provided |
| 3J47 Formation of an intricate helical bundle dictates the assembly of the 26S proteasome lid Deposited 2013-06-27 | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric（8） Consistent with protein count |
Chain O
360–387（28 aa）
Fragment：C-terminal helix (UNP residues 360-387)
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.1
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 7.40 Å |
| 3JCK Structure of the yeast 26S proteasome lid sub-complex Deposited 2015-12-20 | Assembly 1 Protein heterocomplex Heteromer；Protein × 9 PDB declaration: nonameric（9） Consistent with protein count |
Chain F
1–393（393 aa）
|
Not recorded | ZN ZINC ION × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
50 mM HEPES, 100 mM NaCl, 100 mM KCl, 1 mM TCEP；pH 7.5；50 mM HEPES, 100 mM NaCl, 100 mM KCl, 1 mM TCEP
cryo-EM vitrification conditions
4 uL sample was applied to the grid, blotted for 2 seconds, and plunged into liquid ethane.；85 K；Cryogen ETHANE；4 uL sample was applied to the grid, blotted for 2 seconds at 4 degrees C, and plunged into liquid ethane using a manual plunger.
|
Resolution 3.50 Å |
| 3JCO Structure of yeast 26S proteasome in M1 state derived from Titan dataset Deposited 2016-01-06 | Assembly 1 Protein heterocomplex Heteromer；Protein × 47 PDB declaration: 47-meric（47） Consistent with protein count |
Chain O
1–393（393 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
50mM Tris pH 7.5, 100mM NaCl, 5mM MgCl2, 2mM ATP；pH 7.5；50mM Tris pH 7.5, 100mM NaCl, 5mM MgCl2, 2mM ATP
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.80 Å |
| 3JCP Structure of yeast 26S proteasome in M2 state derived from Titan dataset Deposited 2016-01-06 | Assembly 1 Protein heterocomplex Heteromer；Protein × 47 PDB declaration: 47-meric（47） Consistent with protein count |
Chain O
1–393（393 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
50mM Tris pH 7.5, 100mM NaCl, 5mM MgCl2, 2mM ATP；pH 7.5；50mM Tris pH 7.5, 100mM NaCl, 5mM MgCl2, 2mM ATP
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.60 Å |
| 4CR2 Deep classification of a large cryo-EM dataset defines the conformational landscape of the 26S proteasome Deposited 2014-02-25 | Assembly 1 Protein heterocomplex Heteromer；Protein × 33 PDB declaration: 33-meric（33） Consistent with protein count |
Chain O
1–393（393 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.1
cryo-EM vitrification conditions
Cryogen ETHANE；VITRIFICATION 1 -- CRYOGEN- ETHANE, INSTRUMENT- HOMEMADE PLUNGER,
|
Resolution 7.70 Å |
| 4CR3 Deep classification of a large cryo-EM dataset defines the conformational landscape of the 26S proteasome Deposited 2014-02-25 | Assembly 1 Protein heterocomplex Heteromer；Protein × 33 PDB declaration: 33-meric（33） Consistent with protein count |
Chain O
1–393（393 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.1
cryo-EM vitrification conditions
Cryogen ETHANE；VITRIFICATION 1 -- CRYOGEN- ETHANE, INSTRUMENT- HOMEMADE PLUNGER,
|
Resolution 9.30 Å |
| 4CR4 Deep classification of a large cryo-EM dataset defines the conformational landscape of the 26S proteasome Deposited 2014-02-25 | Assembly 1 Protein heterocomplex Heteromer；Protein × 33 PDB declaration: 33-meric（33） Consistent with protein count |
Chain O
1–393（393 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.1
cryo-EM vitrification conditions
Cryogen ETHANE；VITRIFICATION 1 -- CRYOGEN- ETHANE, INSTRUMENT- HOMEMADE PLUNGER,
|
Resolution 8.80 Å |
| 5A5B Structure of the 26S proteasome-Ubp6 complex Deposited 2015-06-17 | Assembly 1 Protein heterocomplex Heteromer；Protein × 35 PDB declaration: 35-meric（35） Consistent with protein count |
Chain O
1–393（393 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM vitrification conditions
Cryogen ETHANE；VITRIFICATION 1 -- CRYOGEN- ETHANE, INSTRUMENT- HOMEMADE PLUNGER,
|
Resolution 9.50 Å |
| 5MPB 26S proteasome in presence of AMP-PNP (s3) Deposited 2016-12-16 | Assembly 1 Protein heterocomplex Heteromer；Protein × 47 PDB declaration: 47-meric（47） Consistent with protein count |
Chain O
1–393（393 aa）
|
Not recorded | ANP PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER × 5 MG MAGNESIUM ION × 6 ADP ADENOSINE-5'-DIPHOSPHATE × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE-PROPANE
|
Resolution 7.80 Å |
| 5MPC 26S proteasome in presence of BeFx (s4) Deposited 2016-12-16 | Assembly 1 Protein heterocomplex Heteromer；Protein × 48 PDB declaration: 48-meric（48） Consistent with protein count |
Chain O
1–393（393 aa）
|
Not recorded | ATP ADENOSINE-5'-TRIPHOSPHATE × 4 MG MAGNESIUM ION × 6 ADP ADENOSINE-5'-DIPHOSPHATE × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE-PROPANE
|
Resolution 7.70 Å |
| 5MPD 26S proteasome in presence of ATP (s1) Deposited 2016-12-16 | Assembly 1 Protein heterocomplex Heteromer；Protein × 13 PDB declaration: tridecameric（13） Consistent with protein count |
Chain O
1–393（393 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE-PROPANE
|
Resolution 4.10 Å |
| 5MPE 26S proteasome in presence of ATP (s2) Deposited 2016-12-16 | Assembly 1 Protein heterocomplex Heteromer；Protein × 13 PDB declaration: tridecameric（13） Consistent with protein count |
Chain O
1–393（393 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE-PROPANE
|
Resolution 4.50 Å |
| 5MPE 26S proteasome in presence of ATP (s2) Deposited 2016-12-16 | Assembly 2 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric（12） Consistent with protein count |
Chain O
1–393（393 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE-PROPANE
|
Resolution 4.50 Å |
| 5WVI The resting state of yeast proteasome Deposited 2016-12-25 | Assembly 1 Protein heterocomplex Heteromer；Protein × 47 PDB declaration: 47-meric（47） Consistent with protein count |
Chain O
1–393（393 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 6.30 Å |
| 5WVK Yeast proteasome-ADP-AlFx Deposited 2016-12-25 | Assembly 1 Protein heterocomplex Heteromer；Protein × 47 PDB declaration: 47-meric（47） Consistent with protein count |
Chain O
1–393（393 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.20 Å |
| 6FVT 26S proteasome, s1 state Deposited 2018-03-05 | Assembly 1 Protein heterocomplex Heteromer；Protein × 47 PDB declaration: 47-meric（47） Consistent with protein count |
Chain O
6–393（388 aa）
|
Not recorded | ATP ADENOSINE-5'-TRIPHOSPHATE × 5 MG MAGNESIUM ION × 6 ADP ADENOSINE-5'-DIPHOSPHATE × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE-PROPANE
|
Resolution 4.10 Å |
| 6FVU 26S proteasome, s2 state Deposited 2018-03-05 | Assembly 1 Protein heterocomplex Heteromer；Protein × 47 PDB declaration: 47-meric（47） Consistent with protein count |
Chain O
6–393（388 aa）
|
Not recorded | ATP ADENOSINE-5'-TRIPHOSPHATE × 5 MG MAGNESIUM ION × 6 ADP ADENOSINE-5'-DIPHOSPHATE × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE-PROPANE
|
Resolution 4.50 Å |
| 6FVV 26S proteasome, s3 state Deposited 2018-03-05 | Assembly 1 Protein heterocomplex Heteromer；Protein × 47 PDB declaration: 47-meric（47） Consistent with protein count |
Chain O
6–393（388 aa）
|
Not recorded | ADP ADENOSINE-5'-DIPHOSPHATE × 2 MG MAGNESIUM ION × 6 ATP ADENOSINE-5'-TRIPHOSPHATE × 4 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE-PROPANE
|
Resolution 5.40 Å |
| 6FVW 26S proteasome, s4 state Deposited 2018-03-05 | Assembly 1 Protein heterocomplex Heteromer；Protein × 47 PDB declaration: 47-meric（47） Consistent with protein count |
Chain O
6–393（388 aa）
|
Not recorded | ATP ADENOSINE-5'-TRIPHOSPHATE × 4 MG MAGNESIUM ION × 6 ADP ADENOSINE-5'-DIPHOSPHATE × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE-PROPANE
|
Resolution 4.50 Å |
| 6FVX 26S proteasome, s5 state Deposited 2018-03-05 | Assembly 1 Protein heterocomplex Heteromer；Protein × 47 PDB declaration: 47-meric（47） Consistent with protein count |
Chain O
6–393（388 aa）
|
Not recorded | ATP ADENOSINE-5'-TRIPHOSPHATE × 5 MG MAGNESIUM ION × 6 ADP ADENOSINE-5'-DIPHOSPHATE × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE-PROPANE
|
Resolution 4.90 Å |
| 6FVY 26S proteasome, s6 state Deposited 2018-03-05 | Assembly 1 Protein heterocomplex Heteromer；Protein × 47 PDB declaration: 47-meric（47） Consistent with protein count |
Chain O
6–393（388 aa）
|
Not recorded | ATP ADENOSINE-5'-TRIPHOSPHATE × 4 MG MAGNESIUM ION × 6 ADP ADENOSINE-5'-DIPHOSPHATE × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE-PROPANE
|
Resolution 6.10 Å |
| 6J2C Yeast proteasome in translocation competent state (C3-a) Deposited 2019-01-01 | Assembly 1 Protein heterocomplex Heteromer；Protein × 47 PDB declaration: 47-meric（47） Consistent with protein count |
Chain O
1–393（393 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 7.00 Å |
| 6J2N yeast proteasome in substrate-processing state (C3-b) Deposited 2019-01-02 | Assembly 1 Protein heterocomplex Heteromer；Protein × 47 PDB declaration: 47-meric（47） Consistent with protein count |
Chain O
1–393（393 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 7.50 Å |
| 6J2Q Yeast proteasome in Ub-accepted state (C1-b) Deposited 2019-01-02 | Assembly 1 Protein heterocomplex Heteromer；Protein × 47 PDB declaration: 47-meric（47） Consistent with protein count |
Chain O
1–393（393 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.80 Å |
| 6J2X Yeast proteasome in resting state (C1-a) Deposited 2019-01-03 | Assembly 1 Protein heterocomplex Heteromer；Protein × 47 PDB declaration: 47-meric（47） Consistent with protein count |
Chain O
1–393（393 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.80 Å |
| 6J30 yeast proteasome in Ub-engaged state (C2) Deposited 2019-01-03 | Assembly 1 Protein heterocomplex Heteromer；Protein × 47 PDB declaration: 47-meric（47） Consistent with protein count |
Chain O
1–393（393 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.50 Å |
| 7QO3 Structure of the 26S proteasome-Ubp6 complex in the si state (Core Particle and Lid) Deposited 2021-12-23 | Assembly 1 Protein heterocomplex Heteromer；Protein × 41 PDB declaration: 41-meric（41） Consistent with protein count |
Chain O
1–393（393 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 6.10 Å |
| 7QO5 26S proteasome Rpt1-RK -Ubp6-UbVS complex in the si state Deposited 2021-12-23 | Assembly 1 Protein heterocomplex Heteromer；Protein × 49 PDB declaration: 49-meric（49） Consistent with protein count |
Chain O
1–393（393 aa）
|
Not recorded | ATP ADENOSINE-5'-TRIPHOSPHATE × 5 MG MAGNESIUM ION × 6 ADP ADENOSINE-5'-DIPHOSPHATE × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 6.00 Å |
| 7QO6 26S proteasome Rpt1-RK -Ubp6-UbVS complex in the s2 state Deposited 2021-12-23 | Assembly 1 Protein heterocomplex Heteromer；Protein × 49 PDB declaration: 49-meric（49） Consistent with protein count |
Chain O
1–393（393 aa）
|
Not recorded | ATP ADENOSINE-5'-TRIPHOSPHATE × 5 MG MAGNESIUM ION × 6 ADP ADENOSINE-5'-DIPHOSPHATE × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 6.30 Å |
| 9CGC Yeast 26S proteasome non-substrate-engaged (S1 state) Deposited 2024-06-28 | Assembly 1 Protein heterocomplex Heteromer；Protein × 39 PDB declaration: 39-meric（39） Consistent with protein count |
Chain O
1–393（393 aa）
|
Not recorded | MG MAGNESIUM ION × 5 ATP ADENOSINE-5'-TRIPHOSPHATE × 5 ZN ZINC ION × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4；30 mM HEPES pH7.4, 25 mM NaCl, 25 mM KCl, 3% (v/v) glycerol, 5 mM MgCl2
2 mM ATP and 0.5 mM TCEP, 0.02% NP-40.
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.61 Å |
| 9IWR 26S proteasome trimer Deposited 2024-07-25 | Assembly 1 Protein heterocomplex Heteromer；Protein × 47 PDB declaration: 47-meric（47） Consistent with protein count |
Chain O
1–393（393 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 6.8；YPD buffer
cryo-EM vitrification conditions
Cryogen ETHANE-PROPANE
|
Resolution 9.10 Å |