Current Protein Identity：Q05127
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Difference tags compare only the current result set; every original PDB and assembly record remains separate.
Related-Structure Differences
Each row represents one biological assembly in one PDB entry; multiple monomers of the same protein are listed separately.
| PDB Entry | Assembly / Oligomeric State | Construct | Mutations and Modifications | Ligands, Ions and Non-polymers | Experimental Method | Experimental Conditions | Structure Quality |
|---|---|---|---|---|---|---|---|
| 3FKE Structure of the Ebola VP35 Interferon Inhibitory Domain Deposited 2008-12-16 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
215–340（126 aa）
Fragment：UNP residues 215-340, interferon inhibitory domain
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5.8；298 K；Well solution: 200 mM Sodium Citrate (pH 5.8),11% (W/V) PEG 4000; protein solution: 20 mM Tris-Cl (pH 7.0),50 mM NaCl, 5mM beta-mercaptoethanol, Vapor Diffusion, Hanging Drop, Temperature 298K, VAPOR DIFFUSION, HANGING DROP
|
Resolution 1.40 Å R-free 0.236 |
| 3FKE Structure of the Ebola VP35 Interferon Inhibitory Domain Deposited 2008-12-16 | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain B
215–340（126 aa）
Fragment：UNP residues 215-340, interferon inhibitory domain
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5.8；298 K；Well solution: 200 mM Sodium Citrate (pH 5.8),11% (W/V) PEG 4000; protein solution: 20 mM Tris-Cl (pH 7.0),50 mM NaCl, 5mM beta-mercaptoethanol, Vapor Diffusion, Hanging Drop, Temperature 298K, VAPOR DIFFUSION, HANGING DROP
|
Resolution 1.40 Å R-free 0.236 |
| 3L25 Crystal structure of Zaire Ebola VP35 interferon inhibitory domain bound to 8 bp dsRNA Deposited 2009-12-14 | Assembly 1 Protein–RNA Homooligomer；Protein × 4 PDB declaration: hexameric（6） Consistent with all polymers |
Chain A
215–340（126 aa）
Fragment：Zaire Ebola VP35 interferon inhibitory domain
Chain B
215–340（126 aa）
Fragment：Zaire Ebola VP35 interferon inhibitory domain
Chain D
215–340（126 aa）
Fragment：Zaire Ebola VP35 interferon inhibitory domain
Chain E
215–340（126 aa）
Fragment：Zaire Ebola VP35 interferon inhibitory domain
|
Not recorded | MG MAGNESIUM ION × 1 CL CHLORIDE ION × 4 NA SODIUM ION × 8 GOL GLYCEROL × 1 FMT FORMIC ACID × 24 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5.2；293 K；3M SODIUM FORMATE, 0.1M SODIUM CITRATE, 5% GLYCEROL, pH 5.2, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.00 Å R-free 0.207 |
| 3L26 Crystal structure of Zaire Ebola VP35 interferon inhibitory domain bound to 8 bp dsRNA Deposited 2009-12-14 | Assembly 1 Protein–RNA Homooligomer；Protein × 4 PDB declaration: hexameric（6） Consistent with all polymers |
Chain A
215–340（126 aa）
Fragment：Zaire Ebola VP35 interferon inhibitory domain
Chain B
215–340（126 aa）
Fragment：Zaire Ebola VP35 interferon inhibitory domain
|
Not recorded | MG MAGNESIUM ION × 10 CL CHLORIDE ION × 10 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 4.7；293 K；2.9M SODIUM FORMATE, 0.1M SODIUM CITRATE, pH 4.7, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.40 Å R-free 0.254 |
| 3L27 Crystal structure of Zaire Ebola VP35 interferon inhibitory domain R312A mutant Deposited 2009-12-14 | Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain A
215–340（126 aa）
Fragment：INTERFERON INHIBITORY DOMAIN
Chain B
215–340（126 aa）
Fragment：INTERFERON INHIBITORY DOMAIN
Chain C
215–340（126 aa）
Fragment：INTERFERON INHIBITORY DOMAIN
Chain D
215–340（126 aa）
Fragment：INTERFERON INHIBITORY DOMAIN
|
Mutation：R312A Mutation：R312A Mutation：R312A Mutation：R312A | CL CHLORIDE ION × 6 PO4 PHOSPHATE ION × 9 GOL GLYCEROL × 10 NA SODIUM ION × 1 K POTASSIUM ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 4.15；293 K；1.85 M SODIUM PHOSPHATE, 0.15 M POTASSIUM PHOSPHATE, pH 4.15, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 1.95 Å R-free 0.229 |
| 3L28 Crystal structure of Zaire Ebola VP35 interferon inhibitory domain K339A mutant Deposited 2009-12-14 | Assembly 1 Protein homooligomer Homooligomer；Protein × 6 PDB declaration: hexameric（6） Consistent with protein count |
Chain A
215–340（126 aa）
Fragment：VP35 INTERFERON INHIBITORY DOMAIN
Chain B
215–340（126 aa）
Fragment：VP35 INTERFERON INHIBITORY DOMAIN
Chain C
215–340（126 aa）
Fragment：VP35 INTERFERON INHIBITORY DOMAIN
Chain D
215–340（126 aa）
Fragment：VP35 INTERFERON INHIBITORY DOMAIN
Chain E
215–340（126 aa）
Fragment：VP35 INTERFERON INHIBITORY DOMAIN
Chain F
215–340（126 aa）
Fragment：VP35 INTERFERON INHIBITORY DOMAIN
|
Mutation：K339A Non-standard monomer：Yes (specific site not provided by mmCIF) Mutation：K339A Non-standard monomer：Yes (specific site not provided by mmCIF) Mutation：K339A Non-standard monomer：Yes (specific site not provided by mmCIF) Mutation：K339A Non-standard monomer：Yes (specific site not provided by mmCIF) Mutation：K339A Non-standard monomer：Yes (specific site not provided by mmCIF) Mutation：K339A Non-standard monomer：Yes (specific site not provided by mmCIF) | SO4 SULFATE ION × 10 CL CHLORIDE ION × 12 NA SODIUM ION × 5 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 2.8；293 K；0.1M SODIUM CITRATE, 0.3M LITHIUM SULFATE, 13% PEG8000, pH 2.8, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.40 Å R-free 0.269 |
| 3L29 Crystal Structure of Zaire Ebola VP35 interferon inhibitory domain K319A/R322A mutant Deposited 2009-12-14 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
215–340（126 aa）
Fragment：Zaire Ebola VP35 interferon inhibitory domain
|
Mutation：K319A, R322A | CL CHLORIDE ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.75；293 K；0.1M BIS-TRIS, 0.2M AMMONIUM SULFATE, 28% PEG3350, pH 6.75, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 1.70 Å R-free 0.232 |
| 3L29 Crystal Structure of Zaire Ebola VP35 interferon inhibitory domain K319A/R322A mutant Deposited 2009-12-14 | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain B
215–340（126 aa）
Fragment：Zaire Ebola VP35 interferon inhibitory domain
|
Mutation：K319A, R322A | CL CHLORIDE ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.75；293 K；0.1M BIS-TRIS, 0.2M AMMONIUM SULFATE, 28% PEG3350, pH 6.75, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 1.70 Å R-free 0.232 |
| 4IBB Ebola virus VP35 bound to small molecule Deposited 2012-12-08 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
215–340（126 aa）
Fragment：unp residues 215-340
|
Not recorded | 1DK {4-[(5R)-3-hydroxy-2-oxo-4-(thiophen-2-ylcarbonyl)-5-(2,4,5-trimethylphenyl)-2,5-dihydro-1H-pyrrol-1-yl]phenyl}acetic acid × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；298 K；100 mM magnesium acetate, pH7.5, 15% PEG3350, 10% DMSO, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 1.75 Å R-free 0.226 |
| 4IBB Ebola virus VP35 bound to small molecule Deposited 2012-12-08 | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain B
215–340（126 aa）
Fragment：unp residues 215-340
|
Not recorded | 1DK {4-[(5R)-3-hydroxy-2-oxo-4-(thiophen-2-ylcarbonyl)-5-(2,4,5-trimethylphenyl)-2,5-dihydro-1H-pyrrol-1-yl]phenyl}acetic acid × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；298 K；100 mM magnesium acetate, pH7.5, 15% PEG3350, 10% DMSO, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 1.75 Å R-free 0.226 |
| 4IBC Ebola virus VP35 bound to small molecule Deposited 2012-12-08 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
215–340（126 aa）
Fragment：unp residues 215-340
|
Not recorded | 12G {4-[(2R)-3-(2-chlorobenzoyl)-2-(2-chlorophenyl)-4-hydroxy-5-oxo-2,5-dihydro-1H-pyrrol-1-yl]phenyl}acetic acid × 1 DMS DIMETHYL SULFOXIDE × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；298 K；100 mM magnesium acetate, pH7.5, 15% PEG3350, 10% DMSO, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 1.75 Å R-free 0.250 |
| 4IBC Ebola virus VP35 bound to small molecule Deposited 2012-12-08 | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain B
215–340（126 aa）
Fragment：unp residues 215-340
|
Not recorded | 12G {4-[(2R)-3-(2-chlorobenzoyl)-2-(2-chlorophenyl)-4-hydroxy-5-oxo-2,5-dihydro-1H-pyrrol-1-yl]phenyl}acetic acid × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；298 K；100 mM magnesium acetate, pH7.5, 15% PEG3350, 10% DMSO, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 1.75 Å R-free 0.250 |
| 4IBD Ebola virus VP35 bound to small molecule Deposited 2012-12-08 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
215–340（126 aa）
Fragment：unp residues 215-340
|
Not recorded | 1DL 5-[(2R)-3-benzoyl-2-(4-bromothiophen-2-yl)-4-hydroxy-5-oxo-2,5-dihydro-1H-pyrrol-1-yl]-2-methylbenzoic acid × 1 GOL GLYCEROL × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5.5；298 K；100 mM sodium citrate, pH5.5, 15% PEG3350, 10%DMSO, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 1.84 Å R-free 0.233 |
| 4IBD Ebola virus VP35 bound to small molecule Deposited 2012-12-08 | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain B
215–340（126 aa）
Fragment：unp residues 215-340
|
Not recorded | 1DL 5-[(2R)-3-benzoyl-2-(4-bromothiophen-2-yl)-4-hydroxy-5-oxo-2,5-dihydro-1H-pyrrol-1-yl]-2-methylbenzoic acid × 1 GOL GLYCEROL × 1 MG MAGNESIUM ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5.5；298 K；100 mM sodium citrate, pH5.5, 15% PEG3350, 10%DMSO, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 1.84 Å R-free 0.233 |
| 4IBE Ebola virus VP35 bound to small molecule Deposited 2012-12-08 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
215–340（126 aa）
Fragment：unp residues 215-340
|
Not recorded | 11Y 5-[(2R)-3-benzoyl-2-(4-bromothiophen-2-yl)-4-hydroxy-5-oxo-2,5-dihydro-1H-pyrrol-1-yl]-2-chlorobenzoic acid × 1 GOL GLYCEROL × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5.5；298 K；100 mM sodium citrate, pH5.5, 15% PEG3350, 10%DMSO, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 1.95 Å R-free 0.233 |
| 4IBE Ebola virus VP35 bound to small molecule Deposited 2012-12-08 | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain B
215–340（126 aa）
Fragment：unp residues 215-340
|
Not recorded | 11Y 5-[(2R)-3-benzoyl-2-(4-bromothiophen-2-yl)-4-hydroxy-5-oxo-2,5-dihydro-1H-pyrrol-1-yl]-2-chlorobenzoic acid × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5.5；298 K；100 mM sodium citrate, pH5.5, 15% PEG3350, 10%DMSO, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 1.95 Å R-free 0.233 |
| 4IBF Ebola virus VP35 bound to small molecule Deposited 2012-12-08 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
215–340（126 aa）
Fragment：unp residues 215-340
|
Not recorded | 1D5 (4-{(2R)-2-(4-bromothiophen-2-yl)-3-[(5-chlorothiophen-2-yl)carbonyl]-4-hydroxy-5-oxo-2,5-dihydro-1H-pyrrol-1-yl}phenyl)acetic acid × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5.5；298 K；100 mM sodium citrate, pH5.5, 15% PEG3350, 10%DMSO, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 2.29 Å R-free 0.260 |
| 4IBF Ebola virus VP35 bound to small molecule Deposited 2012-12-08 | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain B
215–340（126 aa）
Fragment：unp residues 215-340
|
Not recorded | 1D5 (4-{(2R)-2-(4-bromothiophen-2-yl)-3-[(5-chlorothiophen-2-yl)carbonyl]-4-hydroxy-5-oxo-2,5-dihydro-1H-pyrrol-1-yl}phenyl)acetic acid × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5.5；298 K；100 mM sodium citrate, pH5.5, 15% PEG3350, 10%DMSO, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 2.29 Å R-free 0.260 |
| 4IBG Ebola virus VP35 bound to small molecule Deposited 2012-12-08 | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
215–340（126 aa）
Fragment：unp residues 215-340
Chain B
215–340（126 aa）
Fragment：unp residues 215-340
|
Not recorded | 1D6 {4-[(2S)-2-(7-chloro-1,3-benzodioxol-5-yl)-4-hydroxy-3-(3-methylbenzoyl)-5-oxo-2,5-dihydro-1H-pyrrol-1-yl]phenyl}acetic acid × 2 SO4 SULFATE ION × 4 GOL GLYCEROL × 3 PO4 PHOSPHATE ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5.5；298 K；100 mM sodium citrate, pH5.5, 15% PEG3350, 10%DMSO, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 1.41 Å R-free 0.200 |
| 4IBI Ebola virus VP35 bound to small molecule Deposited 2012-12-08 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
215–340（126 aa）
Fragment：unp residues 215-340
|
Not recorded | 1D8 3-{(2S)-2-(7-chloro-1,3-benzodioxol-5-yl)-4-hydroxy-5-oxo-3-[3-(trifluoromethyl)benzoyl]-2,5-dihydro-1H-pyrrol-1-yl}benzoic acid × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5.5；298 K；100 mM sodium citrate, pH5.5, 15% PEG3350, 10%DMS, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 1.47 Å R-free 0.237 |
| 4IBI Ebola virus VP35 bound to small molecule Deposited 2012-12-08 | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain B
215–340（126 aa）
Fragment：unp residues 215-340
|
Not recorded | 1D8 3-{(2S)-2-(7-chloro-1,3-benzodioxol-5-yl)-4-hydroxy-5-oxo-3-[3-(trifluoromethyl)benzoyl]-2,5-dihydro-1H-pyrrol-1-yl}benzoic acid × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5.5；298 K；100 mM sodium citrate, pH5.5, 15% PEG3350, 10%DMS, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 1.47 Å R-free 0.237 |
| 4IBJ Ebola virus VP35 bound to small molecule Deposited 2012-12-08 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
215–340（126 aa）
Fragment：unp residues 215-340
|
Not recorded | 1D9 3-{(5S)-3-hydroxy-2-oxo-4-[3-(trifluoromethyl)benzoyl]-5-[3-(trifluoromethyl)phenyl]-2,5-dihydro-1H-pyrrol-1-yl}benzoic acid × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5.5；298 K；100 mM sodium citrate, pH5.5, 15% PEG3350, 10%DMSO, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 1.54 Å R-free 0.229 |
| 4IBJ Ebola virus VP35 bound to small molecule Deposited 2012-12-08 | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain B
215–340（126 aa）
Fragment：unp residues 215-340
|
Not recorded | 1D9 3-{(5S)-3-hydroxy-2-oxo-4-[3-(trifluoromethyl)benzoyl]-5-[3-(trifluoromethyl)phenyl]-2,5-dihydro-1H-pyrrol-1-yl}benzoic acid × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5.5；298 K；100 mM sodium citrate, pH5.5, 15% PEG3350, 10%DMSO, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 1.54 Å R-free 0.229 |
| 4IBK Ebola virus VP35 bound to small molecule Deposited 2012-12-08 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
215–340（126 aa）
Fragment：unp residues 215-340
|
Not recorded | 1DE 3-{(2S)-2-(7-chloro-1,3-benzodioxol-5-yl)-3-[(5-chlorothiophen-2-yl)carbonyl]-4-hydroxy-5-oxo-2,5-dihydro-1H-pyrrol-1-yl}benzoic acid × 1 GOL GLYCEROL × 1 DMS DIMETHYL SULFOXIDE × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5.5；298 K；100 mM sodium citrate, pH5.5, 15% PEG3350, 10%DMSO, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 1.85 Å R-free 0.241 |
| 4IBK Ebola virus VP35 bound to small molecule Deposited 2012-12-08 | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain B
215–340（126 aa）
Fragment：unp residues 215-340
|
Not recorded | 1DE 3-{(2S)-2-(7-chloro-1,3-benzodioxol-5-yl)-3-[(5-chlorothiophen-2-yl)carbonyl]-4-hydroxy-5-oxo-2,5-dihydro-1H-pyrrol-1-yl}benzoic acid × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5.5；298 K；100 mM sodium citrate, pH5.5, 15% PEG3350, 10%DMSO, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 1.85 Å R-free 0.241 |
| 4IJE Crystal structure of the Zaire ebolavirus VP35 interferon inhibitory domain R312A/K319A/R322A mutant Deposited 2012-12-21 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
218–340（123 aa）
Fragment：interferon inhibitory domain (UNP residues 218-340)
|
Mutation：R312A, K319A, R322A | NA SODIUM ION × 1 K POTASSIUM ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 4.5；298.15 K；2.2 M sodium potassium phosphate, pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 298.15K
|
Resolution 1.90 Å R-free 0.228 |
| 4IJE Crystal structure of the Zaire ebolavirus VP35 interferon inhibitory domain R312A/K319A/R322A mutant Deposited 2012-12-21 | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain B
218–340（123 aa）
Fragment：interferon inhibitory domain (UNP residues 218-340)
|
Mutation：R312A, K319A, R322A | NA SODIUM ION × 2 PO4 PHOSPHATE ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 4.5；298.15 K；2.2 M sodium potassium phosphate, pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 298.15K
|
Resolution 1.90 Å R-free 0.228 |
| 4IJE Crystal structure of the Zaire ebolavirus VP35 interferon inhibitory domain R312A/K319A/R322A mutant Deposited 2012-12-21 | Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain C
218–340（123 aa）
Fragment：interferon inhibitory domain (UNP residues 218-340)
|
Mutation：R312A, K319A, R322A | PO4 PHOSPHATE ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 4.5；298.15 K；2.2 M sodium potassium phosphate, pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 298.15K
|
Resolution 1.90 Å R-free 0.228 |
| 4IJE Crystal structure of the Zaire ebolavirus VP35 interferon inhibitory domain R312A/K319A/R322A mutant Deposited 2012-12-21 | Assembly 4 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain D
218–340（123 aa）
Fragment：interferon inhibitory domain (UNP residues 218-340)
|
Mutation：R312A, K319A, R322A | NA SODIUM ION × 2 PO4 PHOSPHATE ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 4.5；298.15 K；2.2 M sodium potassium phosphate, pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 298.15K
|
Resolution 1.90 Å R-free 0.228 |
| 4IJF Crystal structure of the Zaire ebolavirus VP35 interferon inhibitory domain K222A/R225A/K248A/K251A mutant Deposited 2012-12-21 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
218–340（123 aa）
Fragment：interferon inhibitory domain (UNP residues 218-340)
|
Mutation：K222A, R225A, K248A, K251A | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；298.15 K；0.2 M magnesium chloride, 0.1 M HEPES, pH 7.5, 30% PEG400, VAPOR DIFFUSION, HANGING DROP, temperature 298.15K
|
Resolution 2.51 Å R-free 0.254 |
| 4YPI Structure of Ebola virus nucleoprotein N-terminal fragment bound to a peptide derived from Ebola VP35 Deposited 2015-03-13 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain G
20–47（28 aa）
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；293 K；streak seeding in well solution containing 100 mM Tris pH 7.2, 50 mM Hepes pH 7, and 23% PEG400
|
Resolution 3.71 Å R-free 0.285 |
| 4YPI Structure of Ebola virus nucleoprotein N-terminal fragment bound to a peptide derived from Ebola VP35 Deposited 2015-03-13 | Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain E
20–47（28 aa）
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；293 K；streak seeding in well solution containing 100 mM Tris pH 7.2, 50 mM Hepes pH 7, and 23% PEG400
|
Resolution 3.71 Å R-free 0.285 |
| 4YPI Structure of Ebola virus nucleoprotein N-terminal fragment bound to a peptide derived from Ebola VP35 Deposited 2015-03-13 | Assembly 3 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain F
20–47（28 aa）
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；293 K；streak seeding in well solution containing 100 mM Tris pH 7.2, 50 mM Hepes pH 7, and 23% PEG400
|
Resolution 3.71 Å R-free 0.285 |
| 4YPI Structure of Ebola virus nucleoprotein N-terminal fragment bound to a peptide derived from Ebola VP35 Deposited 2015-03-13 | Assembly 4 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain H
20–47（28 aa）
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；293 K；streak seeding in well solution containing 100 mM Tris pH 7.2, 50 mM Hepes pH 7, and 23% PEG400
|
Resolution 3.71 Å R-free 0.285 |
| 4ZTA Ebola virus nucleoprotein bound to VP35 chaperoning peptide I212121 Deposited 2015-05-14 | Assembly 1 Insufficient information Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
15–59（45 aa）
Fragment：UNP Q05127 residues 15-59,UNP P18272 residues 33-367,UNP Q05127 residues 15-59,UNP P18272 residues 33-367
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 4.7；277 K；2.2M Sodium formate, 100mM NaOAc pH 4.7
|
Resolution 2.40 Å R-free 0.233 |
| 4ZTG Ebola virus nucleoprotein bound to VP35 chaperoning peptide P22121 Deposited 2015-05-14 | Assembly 1 Insufficient information Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
15–59（45 aa）
Fragment：UNP Q05127 residues 15-59, UNP P18272 residues 33-367
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 5；277 K；12% PEG 6000, 100 mM MES pH 5.0
|
Resolution 2.80 Å R-free 0.293 |
| 4ZTI Ebola virus nucleoprotein bound to VP35 chaperoning peptide P212121 Deposited 2015-05-14 | Assembly 1 Insufficient information Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
15–59（45 aa）
Fragment：UNP Q05127 residues 15-59,UNP P18272 residues 33-367
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 4.4；277 K；2.0 M sodium formate, 100 mM sodium acetate pH 4.4
|
Resolution 2.40 Å R-free 0.247 |
| 4ZTI Ebola virus nucleoprotein bound to VP35 chaperoning peptide P212121 Deposited 2015-05-14 | Assembly 2 Insufficient information Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain B
15–59（45 aa）
Fragment：UNP Q05127 residues 15-59,UNP P18272 residues 33-367
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 4.4；277 K；2.0 M sodium formate, 100 mM sodium acetate pH 4.4
|
Resolution 2.40 Å R-free 0.247 |
| 6GBO Crystal Structure of the oligomerization domain of Vp35 from Ebola virus Deposited 2018-04-16 | Assembly 1 Protein homooligomer Homooligomer；Protein × 6 PDB declaration: hexameric（6） Consistent with protein count |
Chain A
82–145（64 aa）
Fragment：oligomerization domain
Chain B
82–145（64 aa）
Fragment：oligomerization domain
Chain C
82–145（64 aa）
Fragment：oligomerization domain
Chain D
82–145（64 aa）
Fragment：oligomerization domain
Chain E
82–145（64 aa）
Fragment：oligomerization domain
Chain F
82–145（64 aa）
Fragment：oligomerization domain
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.5；296 K；50 mM HEPES-NaOH pH 7.5, 2.5 M Na-acetate
|
Resolution 2.10 Å R-free 0.242 |
| 6GBO Crystal Structure of the oligomerization domain of Vp35 from Ebola virus Deposited 2018-04-16 | Assembly 2 Protein homooligomer Homooligomer；Protein × 6 PDB declaration: hexameric（6） Consistent with protein count |
Chain G
82–145（64 aa）
Fragment：oligomerization domain
Chain H
82–145（64 aa）
Fragment：oligomerization domain
Chain I
82–145（64 aa）
Fragment：oligomerization domain
Chain J
82–145（64 aa）
Fragment：oligomerization domain
Chain K
82–145（64 aa）
Fragment：oligomerization domain
Chain L
82–145（64 aa）
Fragment：oligomerization domain
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.5；296 K；50 mM HEPES-NaOH pH 7.5, 2.5 M Na-acetate
|
Resolution 2.10 Å R-free 0.242 |
| 6GBP Crystal Structure of the oligomerization domain of VP35 from Ebola virus, mercury derivative Deposited 2018-04-16 | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric（3） Consistent with protein count |
Chain A
82–145（64 aa）
Fragment：oligomerization domain
Chain B
82–145（64 aa）
Fragment：oligomerization domain
Chain C
82–145（64 aa）
Fragment：oligomerization domain
|
Not recorded | HG MERCURY (II) ION × 4 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.5；296 K；50 mM HEPES-NaOH pH 7.5, 2.6 M Na-acetate
|
Resolution 3.49 Å R-free 0.281 |
| 6GBP Crystal Structure of the oligomerization domain of VP35 from Ebola virus, mercury derivative Deposited 2018-04-16 | Assembly 2 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric（3） Consistent with protein count |
Chain D
82–145（64 aa）
Fragment：oligomerization domain
Chain E
82–145（64 aa）
Fragment：oligomerization domain
Chain F
82–145（64 aa）
Fragment：oligomerization domain
|
Not recorded | HG MERCURY (II) ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.5；296 K；50 mM HEPES-NaOH pH 7.5, 2.6 M Na-acetate
|
Resolution 3.49 Å R-free 0.281 |
| 6GBP Crystal Structure of the oligomerization domain of VP35 from Ebola virus, mercury derivative Deposited 2018-04-16 | Assembly 3 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric（3） Consistent with protein count |
Chain G
82–145（64 aa）
Fragment：oligomerization domain
Chain H
82–145（64 aa）
Fragment：oligomerization domain
Chain I
82–145（64 aa）
Fragment：oligomerization domain
|
Not recorded | HG MERCURY (II) ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.5；296 K；50 mM HEPES-NaOH pH 7.5, 2.6 M Na-acetate
|
Resolution 3.49 Å R-free 0.281 |
| 6GBP Crystal Structure of the oligomerization domain of VP35 from Ebola virus, mercury derivative Deposited 2018-04-16 | Assembly 4 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric（3） Consistent with protein count |
Chain J
82–145（64 aa）
Fragment：oligomerization domain
Chain K
82–145（64 aa）
Fragment：oligomerization domain
Chain L
82–145（64 aa）
Fragment：oligomerization domain
|
Not recorded | HG MERCURY (II) ION × 4 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.5；296 K；50 mM HEPES-NaOH pH 7.5, 2.6 M Na-acetate
|
Resolution 3.49 Å R-free 0.281 |
| 8USN Intracellular cryo-tomography structure of EBOV nucleocapsid at 8.9 Angstrom Deposited 2023-10-27 | Assembly 1 Protein–RNA Heteromer；Protein × 7 PDB declaration: nonameric（9） Consistent with all polymers |
Chain F
1–340（340 aa）
Chain K
1–340（340 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.2
cryo-EM vitrification conditions
Cryogen ETHANE-PROPANE
|
Resolution 8.90 Å |
| 8UST In-virion structure of Ebola virus nucleocapsid-like assemblies from recombinant virus-like particles (nucleoprotein, VP24,VP35,VP40) Deposited 2023-10-29 | Assembly 1 Protein–RNA Heteromer；Protein × 7 PDB declaration: nonameric（9） Consistent with all polymers |
Chain F
1–340（340 aa）
Chain K
1–340（340 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.2
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 7.30 Å |
| 9IP3 Cryo-EM structure of the RNA-dependent RNA polymerase complex in a compact conformation from Ebola virus Deposited 2024-07-10 | Assembly 1 Insufficient information Heteromer；Protein × 5 PDB declaration: pentameric（5） Consistent with protein count |
Chain B
80–340（261 aa）
Chain C
80–340（261 aa）
Chain D
80–340（261 aa）
Chain E
80–340（261 aa）
|
Not recorded | ZN ZINC ION × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5；25 mM HEPES, 300 mM NaCl, 1 mM TCEP, 6 mM MgCl2
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.10 Å |