Current Protein Identity:Q8IYB3
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Difference tags compare only the current result set; every original PDB and assembly record remains separate.
Related-Structure Differences
Each row represents one biological assembly in one PDB entry; multiple monomers of the same protein are listed separately.
| PDB Entry | Assembly / Oligomeric State | Construct | Mutations and Modifications | Ligands, Ions and Non-polymers | Experimental Method | Experimental Conditions | Structure Quality |
|---|---|---|---|---|---|---|---|
| 1MP1 Solution structure of the PWI motif from SRm160 Deposited 2002-09-11 | Assembly 1 Protein monomer Monomer;Protein × 1 PDB declaration: monomeric(1) Consistent with protein count |
Chain A
27–134(108 aa)
Fragment:PWI motif (residues 27-134)
|
Not recorded | No recorded non-water small molecule | SOLUTION NMR |
NMR measurement conditions
pH 7;298 K;Ionic strength (raw mmCIF value) 300mM NaCl, 25mM phosphate buffer;Pressure ambient
NMR sample composition
1.4mM PWI_motif U-15N,13C, 300mM NaCl, 25 mM phosphate buffer, 1mM DTT, 1mM inhibitor cocktail | 90% H20, 10%D2O
|
Resolution not provided |
| 6FF4 human Bact spliceosome core structure Deposited 2018-01-03 | Assembly 1 Protein–RNA Heteromer;Protein × 24 PDB declaration: 28-meric(28) Consistent with all polymers |
Chain Y
1–904(904 aa)
|
Not recorded | MG MAGNESIUM ION × 4 IHP INOSITOL HEXAKISPHOSPHATE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ZN ZINC ION × 10 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.9
cryo-EM vitrification conditions
Cryogen ETHANE;blot with blotting sensor
|
Resolution 3.40 Å |
| 6FF7 human Bact spliceosome core structure Deposited 2018-01-03 | Assembly 1 Protein–RNA Heteromer;Protein × 57 PDB declaration: 61-meric(61) Consistent with all polymers |
Chain Y
1–904(904 aa)
|
Not recorded | ZN ZINC ION × 11 MG MAGNESIUM ION × 5 IHP INOSITOL HEXAKISPHOSPHATE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.50 Å |
| 7ABG Human pre-Bact-1 spliceosome Deposited 2020-09-07 | Assembly 1 Protein–RNA Heteromer;Protein × 54 PDB declaration: 58-meric(58) Consistent with all polymers |
Chain Y
1–904(904 aa)
|
Not recorded | IHP INOSITOL HEXAKISPHOSPHATE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 GTG 7-METHYL-GUANOSINE-5'-TRIPHOSPHATE-5'-GUANOSINE × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.9
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 7.80 Å |
| 7ABH Human pre-Bact-2 spliceosome (SF3b/U2 snRNP portion) Deposited 2020-09-07 | Assembly 1 Protein–RNA Heteromer;Protein × 14 PDB declaration: hexadecameric(16) Consistent with all polymers |
Chain Y
1–904(904 aa)
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.9
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.50 Å |
| 7ABI Human pre-Bact-2 spliceosome Deposited 2020-09-07 | Assembly 1 Protein–RNA Heteromer;Protein × 52 PDB declaration: 56-meric(56) Consistent with all polymers |
Chain Y
1–904(904 aa)
|
Not recorded | GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 IHP INOSITOL HEXAKISPHOSPHATE × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.9
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 8.00 Å |
| 7DVQ Cryo-EM Structure of the Activated Human Minor Spliceosome (minor Bact Complex) Deposited 2021-01-14 | Assembly 1 Protein–RNA Heteromer;Protein × 45 PDB declaration: 49-meric(49) Consistent with all polymers |
Chain 8
1–904(904 aa)
|
Not recorded | IHP INOSITOL HEXAKISPHOSPHATE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 5 K POTASSIUM ION × 2 G5J 5'-O-[(S)-hydroxy{[(R)-hydroxy{[(S)-hydroxy(methoxy)phosphoryl]oxy}phosphoryl]oxy}phosphoryl]guanosine × 1 ZN ZINC ION × 7 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.9
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.89 Å |
| 8I0P The cryo-EM structure of human pre-Bact complex Deposited 2023-01-11 | Assembly 1 Protein–RNA Heteromer;Protein × 47 PDB declaration: 51-meric(51) Consistent with all polymers |
Chain 8
1–904(904 aa)
|
Not recorded | GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ZN ZINC ION × 4 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.9
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.40 Å |
| 8I0R The cryo-EM structure of human Bact-I complex Deposited 2023-01-11 | Assembly 1 Protein–RNA Heteromer;Protein × 51 PDB declaration: 55-meric(55) Consistent with all polymers |
Chain 8
1–904(904 aa)
|
Not recorded | IHP INOSITOL HEXAKISPHOSPHATE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 6 ZN ZINC ION × 7 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.9
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.00 Å |