Current Protein Identity：Q8WZ42
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Difference tags compare only the current result set; every original PDB and assembly record remains separate.
Related-Structure Differences
Each row represents one biological assembly in one PDB entry; multiple monomers of the same protein are listed separately.
| PDB Entry | Assembly / Oligomeric State | Construct | Mutations and Modifications | Ligands, Ions and Non-polymers | Experimental Method | Experimental Conditions | Structure Quality |
|---|---|---|---|---|---|---|---|
| 1TNM TERTIARY STRUCTURE OF AN IMMUNOGLOBULIN-LIKE DOMAIN FROM THE GIANT MUSCLE PROTEIN TITIN: A NEW MEMBER OF THE I SET Deposited 1995-01-17 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
33489–33579（91 aa）
|
Not recorded | No recorded non-water small molecule | SOLUTION NMR | mmCIF provides none of the parsed conditions | Resolution not provided |
| 1TNN Tertiary structure of an immunoglobulin-like domain from the giant muscle protein titin: a new member of the I set Deposited 1995-01-17 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
33489–33579（91 aa）
|
Not recorded | No recorded non-water small molecule | SOLUTION NMR | mmCIF provides none of the parsed conditions | Resolution not provided |
| 1WAA IG27 protein domain Deposited 2004-10-25 | Assembly 1 Protein homooligomer Homooligomer；Protein × 6 PDB declaration: hexameric（6） Consistent with protein count |
Chain A
12801–12889（89 aa）
Fragment：IG DOMAIN, RESIDUES 12801-12889
Chain B
12801–12889（89 aa）
Fragment：IG DOMAIN, RESIDUES 12801-12889
Chain C
12801–12889（89 aa）
Fragment：IG DOMAIN, RESIDUES 12801-12889
Chain D
12801–12889（89 aa）
Fragment：IG DOMAIN, RESIDUES 12801-12889
Chain E
12801–12889（89 aa）
Fragment：IG DOMAIN, RESIDUES 12801-12889
Chain F
12801–12889（89 aa）
Fragment：IG DOMAIN, RESIDUES 12801-12889
|
Not recorded | ZN ZINC ION × 24 | X-RAY DIFFRACTION | mmCIF provides none of the parsed conditions | Resolution 1.80 Å R-free 0.268 |
| 2A38 Crystal structure of the N-Terminus of titin Deposited 2005-06-24 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
1–194（194 aa）
Fragment：Ig-like 1/2, residues 1-194
|
Not recorded | CD CADMIUM ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5.5；293 K；MES, Cadmium sulphate, Sodium acetate, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.00 Å R-free 0.255 |
| 2A38 Crystal structure of the N-Terminus of titin Deposited 2005-06-24 | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain B
1–194（194 aa）
Fragment：Ig-like 1/2, residues 1-194
|
Not recorded | CD CADMIUM ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5.5；293 K；MES, Cadmium sulphate, Sodium acetate, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.00 Å R-free 0.255 |
| 2A38 Crystal structure of the N-Terminus of titin Deposited 2005-06-24 | Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain C
1–194（194 aa）
Fragment：Ig-like 1/2, residues 1-194
|
Not recorded | CD CADMIUM ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5.5；293 K；MES, Cadmium sulphate, Sodium acetate, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.00 Å R-free 0.255 |
| 2BK8 M1 domain from titin Deposited 2005-02-11 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
32497–32590（94 aa）
Fragment：RESIDUES 25073-25166 (IG-LIKE DOMAIN)
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 7；2.2 M AMMONIUM SULFATE 0.1 M BIS TRIS PH 7.0
|
Resolution 1.69 Å R-free 0.234 |
| 2F8V Structure of full length telethonin in complex with the N-terminus of titin Deposited 2005-12-04 | Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric（3） Consistent with protein count |
Chain A
1–196（196 aa）
Fragment：Domains Z1Z2, residues 1-196
Chain B
1–196（196 aa）
Fragment：Domains Z1Z2, residues 1-196
|
Not recorded | SO4 SULFATE ION × 3 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 4.45；298 K；PEG35000, LITHIUM SULPHATE, ACETATE BUFFER, pH 4.45, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 2.75 Å R-free 0.287 |
| 2F8V Structure of full length telethonin in complex with the N-terminus of titin Deposited 2005-12-04 | Assembly 2 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric（3） Consistent with protein count |
Chain C
1–196（196 aa）
Fragment：Domains Z1Z2, residues 1-196
Chain D
1–196（196 aa）
Fragment：Domains Z1Z2, residues 1-196
|
Not recorded | SO4 SULFATE ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 4.45；298 K；PEG35000, LITHIUM SULPHATE, ACETATE BUFFER, pH 4.45, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 2.75 Å R-free 0.287 |
| 2ILL Anomalous substructure of Titin-A168169 Deposited 2006-10-03 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
31854–32047（194 aa）
Fragment：Titin-A168169
|
Not recorded | CL CHLORIDE ION × 2 | X-RAY DIFFRACTION | mmCIF provides none of the parsed conditions | Resolution 2.20 Å R-free 0.243 |
| 2J8H Structure of the immunoglobulin tandem repeat A168-A169 of titin Deposited 2006-10-25 | Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain A
24430–24623（194 aa）
Fragment：IG LIKE DOMAIN, RESIDUES 24430-24623
|
Not recorded | GOL GLYCEROL × 4 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 9；AMMONIUM SULFATE, BICINE, pH 9
|
Resolution 1.99 Å R-free 0.210 |
| 2J8O Structure of the immunoglobulin tandem repeat of titin A168-A169 Deposited 2006-10-26 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
24430–24623（194 aa）
Fragment：IG LIKE DOMAIN, RESIDUES 24430-24623
|
Not recorded | GOL GLYCEROL × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 9；AMMONIUM SULFATE, BICINE, pH 9
|
Resolution 2.49 Å R-free 0.300 |
| 2J8O Structure of the immunoglobulin tandem repeat of titin A168-A169 Deposited 2006-10-26 | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain B
24430–24623（194 aa）
Fragment：IG LIKE DOMAIN, RESIDUES 24430-24623
|
Not recorded | GOL GLYCEROL × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 9；AMMONIUM SULFATE, BICINE, pH 9
|
Resolution 2.49 Å R-free 0.300 |
| 2NZI Crystal structure of domains A168-A170 from titin Deposited 2006-11-23 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
31854–32155（302 aa）
Fragment：Residues 31854-32155
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5.5；298 K；2.1M NaCl, 0.1M MES, 0.6M Li2SO4, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 2.90 Å R-free 0.274 |
| 2NZI Crystal structure of domains A168-A170 from titin Deposited 2006-11-23 | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain B
31854–32155（302 aa）
Fragment：Residues 31854-32155
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5.5；298 K；2.1M NaCl, 0.1M MES, 0.6M Li2SO4, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 2.90 Å R-free 0.274 |
| 2RQ8 Solution NMR structure of titin I27 domain mutant Deposited 2009-03-05 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
12677–12765（89 aa）
Fragment：UNP residues 12677-12765
|
Mutation：K3E, Y9P, T42A, A78T | No recorded non-water small molecule | SOLUTION NMR |
NMR measurement conditions
pH 7.4；298 K；Ionic strength (raw mmCIF value) 50mM POTASSIUM PHOSPHATE；Pressure 1
NMR sample composition
1.0mM TITIN I27 mutant, 50mM potassium phosphate buffer, 99.9% D2O | 99.9% D2O
NMR sample composition
1.0mM [U-15N] TITIN I27 mutant, 50mM potassium phosphate buffer, 90% H2O/10% D2O | 90% H2O/10% D2O
NMR sample composition
1.0mM [U-13C; U-15N] TITIN I27 mutant, 50mM potassium phosphate buffer, 99.9% D2O | 99.9% D2O
|
Resolution not provided |
| 2WP3 Crystal structure of the Titin M10-Obscurin like 1 Ig complex Deposited 2009-08-02 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain T
34252–34350（99 aa）
Fragment：M10, RESIDUES 34252-34350
|
Not recorded | GOL GLYCEROL × 4 SO4 SULFATE ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 7；PROTEIN COMPLEX AT 13 MG/ML 2M AMM SULPHATE, 0.1 M BIS-TRIS PH 5.5
|
Resolution 1.48 Å R-free 0.217 |
| 2WWK Crystal structure of the Titin M10-Obscurin like 1 Ig F17R mutant complex Deposited 2009-10-25 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain T
34252–34350（99 aa）
Fragment：M10, RESIDUES 34252-34350
|
Not recorded | GOL GLYCEROL × 1 SO4 SULFATE ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 5.5；0.1 M BIS-TRIS PH 5.5, 0.2 M AMMONIUM SULPHATE, 25% PEG 3350
|
Resolution 1.70 Å R-free 0.259 |
| 2WWM Crystal structure of the Titin M10-Obscurin like 1 Ig complex in space group P1 Deposited 2009-10-26 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain T
34252–34350（99 aa）
Fragment：M10, RESIDUES 34252-34350
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 5；0.1 M MIB BUFFER PH 5.0, 25% PEG 1500
|
Resolution 2.30 Å R-free 0.252 |
| 2WWM Crystal structure of the Titin M10-Obscurin like 1 Ig complex in space group P1 Deposited 2009-10-26 | Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain D
34252–34350（99 aa）
Fragment：M10, RESIDUES 34252-34350
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 5；0.1 M MIB BUFFER PH 5.0, 25% PEG 1500
|
Resolution 2.30 Å R-free 0.252 |
| 2Y9R Crystal structure of the M10 domain of Titin Deposited 2011-02-16 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain T
26828–26926（99 aa）
Fragment：M10 DOMAIN, RESIDUES 26828-26926
|
Not recorded | 12P DODECAETHYLENE GLYCOL × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 6.5；35% PEG 400, 0.1M BIS-TRIS PH 6.5
|
Resolution 1.90 Å R-free 0.241 |
| 3KNB Crystal structure of the titin C-terminus in complex with obscurin-like 1 Deposited 2009-11-12 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
34253–34350（98 aa）
Fragment：titin, C-terminal domain M10, resideus 34253-34350
|
Not recorded | SO4 SULFATE ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8；293 K；ammonium sulfate, imidazole, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 1.40 Å R-free 0.195 |
| 3LCY Titin Ig tandem domains A164-A165 Deposited 2010-01-12 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
31456–31649（194 aa）
Fragment：Titin A-band tandem Ig domains A164-A165
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；293 K；0.1M Hepes PH 7.5, 10% iso-propanol
8.5% PEG20,000, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.50 Å R-free 0.240 |
| 3LCY Titin Ig tandem domains A164-A165 Deposited 2010-01-12 | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain B
31456–31649（194 aa）
Fragment：Titin A-band tandem Ig domains A164-A165
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；293 K；0.1M Hepes PH 7.5, 10% iso-propanol
8.5% PEG20,000, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.50 Å R-free 0.240 |
| 3LCY Titin Ig tandem domains A164-A165 Deposited 2010-01-12 | Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain C
31456–31649（194 aa）
Fragment：Titin A-band tandem Ig domains A164-A165
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；293 K；0.1M Hepes PH 7.5, 10% iso-propanol
8.5% PEG20,000, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.50 Å R-free 0.240 |
| 3LCY Titin Ig tandem domains A164-A165 Deposited 2010-01-12 | Assembly 4 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain D
31456–31649（194 aa）
Fragment：Titin A-band tandem Ig domains A164-A165
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；293 K；0.1M Hepes PH 7.5, 10% iso-propanol
8.5% PEG20,000, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.50 Å R-free 0.240 |
| 3LPW Crystal structure of the FnIII-tandem A77-A78 from the A-band of titin Deposited 2010-02-06 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
22877–23070（194 aa）
|
Not recorded | MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 1 MRD (4R)-2-METHYLPENTANE-2,4-DIOL × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；277 K；VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 1.65 Å R-free 0.208 |
| 3LPW Crystal structure of the FnIII-tandem A77-A78 from the A-band of titin Deposited 2010-02-06 | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain B
22877–23070（194 aa）
|
Not recorded | MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；277 K；VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 1.65 Å R-free 0.208 |
| 3PUC Atomic resolution structure of titin domain M7 Deposited 2010-12-04 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
33774–33871（98 aa）
Fragment：domain M7 (UNP RESIDUES 33774-33871)
|
Not recorded | SO4 SULFATE ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8；293 K；0.1M Tris pH 8.0, 3.2M ammonium sulfate, 1mM TCEP, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 0.96 Å R-free 0.135 |
| 3Q5O Crystal structure of human titin domain M10 Deposited 2010-12-29 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
34253–34350（98 aa）
Fragment：domain M10 (UNP RESIDUES 34253-34350)
|
Not recorded | SO4 SULFATE ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；277 K；0.1M bistris p 6.8, 55% MPD, 0.2M magnesium sulfate, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 2.05 Å R-free 0.215 |
| 3Q5O Crystal structure of human titin domain M10 Deposited 2010-12-29 | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain B
34253–34350（98 aa）
Fragment：domain M10 (UNP RESIDUES 34253-34350)
|
Not recorded | SO4 SULFATE ION × 3 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；277 K；0.1M bistris p 6.8, 55% MPD, 0.2M magnesium sulfate, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 2.05 Å R-free 0.215 |
| 3QP3 Crystal structure of titin domain M4, tetragonal form Deposited 2011-02-11 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
33294–33395（102 aa）
Fragment：domain M4 (UNP RESIDUES 33294-33395)
|
Not recorded | SO4 SULFATE ION × 5 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 4；293 K；0.2M Na-citrate pH 4.0, 1.2M (NH4)2SO4, 0.3M MgSO4, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.00 Å R-free 0.213 |
| 3QP3 Crystal structure of titin domain M4, tetragonal form Deposited 2011-02-11 | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain B
33294–33395（102 aa）
Fragment：domain M4 (UNP RESIDUES 33294-33395)
|
Not recorded | SO4 SULFATE ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 4；293 K；0.2M Na-citrate pH 4.0, 1.2M (NH4)2SO4, 0.3M MgSO4, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.00 Å R-free 0.213 |
| 3QP3 Crystal structure of titin domain M4, tetragonal form Deposited 2011-02-11 | Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain C
33294–33395（102 aa）
Fragment：domain M4 (UNP RESIDUES 33294-33395)
|
Not recorded | SO4 SULFATE ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 4；293 K；0.2M Na-citrate pH 4.0, 1.2M (NH4)2SO4, 0.3M MgSO4, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.00 Å R-free 0.213 |
| 4C4K Crystal structure of the titin M10-Obscurin Ig domain 1 complex Deposited 2013-09-05 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain T
34252–34350（99 aa）
Fragment：M10 DOMAIN, RESIDUES 34252-34350
|
Not recorded | EDO 1,2-ETHANEDIOL × 12 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 8.5；0.1 M TRIS-HCL PH 8.5, 0.2 M SODIUM ACETATE, 30% PEG4000
|
Resolution 1.95 Å R-free 0.210 |
| 4JNW Bacterially expressed Titin Kinase Deposited 2013-03-16 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
32172–32492（321 aa）
Fragment：titin kinase: unp residues 32172-32492
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；VAPOR DIFFUSION, HANGING DROP
|
Resolution 2.06 Å R-free 0.204 |
| 4JNW Bacterially expressed Titin Kinase Deposited 2013-03-16 | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain B
32172–32492（321 aa）
Fragment：titin kinase: unp residues 32172-32492
|
Not recorded | GOL GLYCEROL × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；VAPOR DIFFUSION, HANGING DROP
|
Resolution 2.06 Å R-free 0.204 |
| 4O00 Crystal structure of the Titin A-band domain A3 Deposited 2013-12-13 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
14217–14319（103 aa）
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 4；291 K；0.1M SPG buffer, 0.01M Tris-HCl, 31% PEG1500, 50mM NaCl, 1mM DTT, pH 4.0, VAPOR DIFFUSION, SITTING DROP, temperature 291K
|
Resolution 1.85 Å R-free 0.208 |
| 4O00 Crystal structure of the Titin A-band domain A3 Deposited 2013-12-13 | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain B
14217–14319（103 aa）
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 4；291 K；0.1M SPG buffer, 0.01M Tris-HCl, 31% PEG1500, 50mM NaCl, 1mM DTT, pH 4.0, VAPOR DIFFUSION, SITTING DROP, temperature 291K
|
Resolution 1.85 Å R-free 0.208 |
| 4QEG Crystal structure of domain I10 from titin (space group P41) Deposited 2014-05-16 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
2880–2967（88 aa）
Fragment：domain I10
|
Not recorded | CA CALCIUM ION × 3 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.5；0.2 M CaCl2, 30% PEG 3350, 0.1 M Tris, 3% Isopropanol, pH 7.5, VAPOR DIFFUSION, SITTING DROP
|
Resolution 2.00 Å R-free 0.231 |
| 4UOW Crystal structure of the titin M10-Obscurin Ig domain 1 complex Deposited 2014-06-10 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain Y
34253–34349（97 aa）
Fragment：FIRST IG DOMAIN, RESIDUES 26829-26925
|
Not recorded | CL CHLORIDE ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
MICROBATCH；pH 3.6；291 K；3.0 M SODIUM CHLORIDE 0.1 M TRI-SODIUM CITRATE PH 3.6 BATCH SETUP
|
Resolution 3.30 Å R-free 0.210 |
| 4UOW Crystal structure of the titin M10-Obscurin Ig domain 1 complex Deposited 2014-06-10 | Assembly 10 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain O
34253–34349（97 aa）
Fragment：FIRST IG DOMAIN, RESIDUES 26829-26925
|
Not recorded | NA SODIUM ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
MICROBATCH；pH 3.6；291 K；3.0 M SODIUM CHLORIDE 0.1 M TRI-SODIUM CITRATE PH 3.6 BATCH SETUP
|
Resolution 3.30 Å R-free 0.210 |
| 4UOW Crystal structure of the titin M10-Obscurin Ig domain 1 complex Deposited 2014-06-10 | Assembly 11 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain K
34253–34349（97 aa）
Fragment：FIRST IG DOMAIN, RESIDUES 26829-26925
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
MICROBATCH；pH 3.6；291 K；3.0 M SODIUM CHLORIDE 0.1 M TRI-SODIUM CITRATE PH 3.6 BATCH SETUP
|
Resolution 3.30 Å R-free 0.210 |
| 4UOW Crystal structure of the titin M10-Obscurin Ig domain 1 complex Deposited 2014-06-10 | Assembly 12 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain U
34253–34349（97 aa）
Fragment：FIRST IG DOMAIN, RESIDUES 26829-26925
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
MICROBATCH；pH 3.6；291 K；3.0 M SODIUM CHLORIDE 0.1 M TRI-SODIUM CITRATE PH 3.6 BATCH SETUP
|
Resolution 3.30 Å R-free 0.210 |
| 4UOW Crystal structure of the titin M10-Obscurin Ig domain 1 complex Deposited 2014-06-10 | Assembly 13 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain G
34253–34349（97 aa）
Fragment：FIRST IG DOMAIN, RESIDUES 26829-26925
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
MICROBATCH；pH 3.6；291 K；3.0 M SODIUM CHLORIDE 0.1 M TRI-SODIUM CITRATE PH 3.6 BATCH SETUP
|
Resolution 3.30 Å R-free 0.210 |
| 4UOW Crystal structure of the titin M10-Obscurin Ig domain 1 complex Deposited 2014-06-10 | Assembly 14 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain Q
34253–34349（97 aa）
Fragment：FIRST IG DOMAIN, RESIDUES 26829-26925
|
Not recorded | CL CHLORIDE ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
MICROBATCH；pH 3.6；291 K；3.0 M SODIUM CHLORIDE 0.1 M TRI-SODIUM CITRATE PH 3.6 BATCH SETUP
|
Resolution 3.30 Å R-free 0.210 |
| 4UOW Crystal structure of the titin M10-Obscurin Ig domain 1 complex Deposited 2014-06-10 | Assembly 15 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain W
34253–34349（97 aa）
Fragment：FIRST IG DOMAIN, RESIDUES 26829-26925
|
Not recorded | CL CHLORIDE ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
MICROBATCH；pH 3.6；291 K；3.0 M SODIUM CHLORIDE 0.1 M TRI-SODIUM CITRATE PH 3.6 BATCH SETUP
|
Resolution 3.30 Å R-free 0.210 |
| 4UOW Crystal structure of the titin M10-Obscurin Ig domain 1 complex Deposited 2014-06-10 | Assembly 16 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain 2
34253–34349（97 aa）
Fragment：FIRST IG DOMAIN, RESIDUES 26829-26925
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
MICROBATCH；pH 3.6；291 K；3.0 M SODIUM CHLORIDE 0.1 M TRI-SODIUM CITRATE PH 3.6 BATCH SETUP
|
Resolution 3.30 Å R-free 0.210 |
| 4UOW Crystal structure of the titin M10-Obscurin Ig domain 1 complex Deposited 2014-06-10 | Assembly 17 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
34253–34349（97 aa）
Fragment：FIRST IG DOMAIN, RESIDUES 26829-26925
|
Not recorded | CL CHLORIDE ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
MICROBATCH；pH 3.6；291 K；3.0 M SODIUM CHLORIDE 0.1 M TRI-SODIUM CITRATE PH 3.6 BATCH SETUP
|
Resolution 3.30 Å R-free 0.210 |
| 4UOW Crystal structure of the titin M10-Obscurin Ig domain 1 complex Deposited 2014-06-10 | Assembly 18 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain S
34253–34349（97 aa）
Fragment：FIRST IG DOMAIN, RESIDUES 26829-26925
|
Not recorded | CL CHLORIDE ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
MICROBATCH；pH 3.6；291 K；3.0 M SODIUM CHLORIDE 0.1 M TRI-SODIUM CITRATE PH 3.6 BATCH SETUP
|
Resolution 3.30 Å R-free 0.210 |
| 4UOW Crystal structure of the titin M10-Obscurin Ig domain 1 complex Deposited 2014-06-10 | Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain M
34253–34349（97 aa）
Fragment：FIRST IG DOMAIN, RESIDUES 26829-26925
|
Not recorded | CL CHLORIDE ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
MICROBATCH；pH 3.6；291 K；3.0 M SODIUM CHLORIDE 0.1 M TRI-SODIUM CITRATE PH 3.6 BATCH SETUP
|
Resolution 3.30 Å R-free 0.210 |
| 4UOW Crystal structure of the titin M10-Obscurin Ig domain 1 complex Deposited 2014-06-10 | Assembly 3 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain E
34253–34349（97 aa）
Fragment：FIRST IG DOMAIN, RESIDUES 26829-26925
|
Not recorded | CL CHLORIDE ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
MICROBATCH；pH 3.6；291 K；3.0 M SODIUM CHLORIDE 0.1 M TRI-SODIUM CITRATE PH 3.6 BATCH SETUP
|
Resolution 3.30 Å R-free 0.210 |
| 4UOW Crystal structure of the titin M10-Obscurin Ig domain 1 complex Deposited 2014-06-10 | Assembly 4 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain 8
34253–34349（97 aa）
Fragment：FIRST IG DOMAIN, RESIDUES 26829-26925
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
MICROBATCH；pH 3.6；291 K；3.0 M SODIUM CHLORIDE 0.1 M TRI-SODIUM CITRATE PH 3.6 BATCH SETUP
|
Resolution 3.30 Å R-free 0.210 |
| 4UOW Crystal structure of the titin M10-Obscurin Ig domain 1 complex Deposited 2014-06-10 | Assembly 5 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain 6
34253–34349（97 aa）
Fragment：FIRST IG DOMAIN, RESIDUES 26829-26925
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
MICROBATCH；pH 3.6；291 K；3.0 M SODIUM CHLORIDE 0.1 M TRI-SODIUM CITRATE PH 3.6 BATCH SETUP
|
Resolution 3.30 Å R-free 0.210 |
| 4UOW Crystal structure of the titin M10-Obscurin Ig domain 1 complex Deposited 2014-06-10 | Assembly 6 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain 0
34253–34349（97 aa）
Fragment：FIRST IG DOMAIN, RESIDUES 26829-26925
|
Not recorded | CL CHLORIDE ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
MICROBATCH；pH 3.6；291 K；3.0 M SODIUM CHLORIDE 0.1 M TRI-SODIUM CITRATE PH 3.6 BATCH SETUP
|
Resolution 3.30 Å R-free 0.210 |
| 4UOW Crystal structure of the titin M10-Obscurin Ig domain 1 complex Deposited 2014-06-10 | Assembly 7 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain I
34253–34349（97 aa）
Fragment：FIRST IG DOMAIN, RESIDUES 26829-26925
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
MICROBATCH；pH 3.6；291 K；3.0 M SODIUM CHLORIDE 0.1 M TRI-SODIUM CITRATE PH 3.6 BATCH SETUP
|
Resolution 3.30 Å R-free 0.210 |
| 4UOW Crystal structure of the titin M10-Obscurin Ig domain 1 complex Deposited 2014-06-10 | Assembly 8 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain C
34253–34349（97 aa）
Fragment：FIRST IG DOMAIN, RESIDUES 26829-26925
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
MICROBATCH；pH 3.6；291 K；3.0 M SODIUM CHLORIDE 0.1 M TRI-SODIUM CITRATE PH 3.6 BATCH SETUP
|
Resolution 3.30 Å R-free 0.210 |
| 4UOW Crystal structure of the titin M10-Obscurin Ig domain 1 complex Deposited 2014-06-10 | Assembly 9 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain 4
34253–34349（97 aa）
Fragment：FIRST IG DOMAIN, RESIDUES 26829-26925
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
MICROBATCH；pH 3.6；291 K；3.0 M SODIUM CHLORIDE 0.1 M TRI-SODIUM CITRATE PH 3.6 BATCH SETUP
|
Resolution 3.30 Å R-free 0.210 |
| 5BS0 MAGE-A3 Reactive TCR in complex with Titin Epitope in HLA-A1 Deposited 2015-06-01 | Assembly 1 Insufficient information Heteromer；Protein × 5 PDB declaration: pentameric（5） Consistent with protein count |
Chain C
24337–24345（9 aa）
Fragment：UNP residues 24337-24345
|
Not recorded | GOL GLYCEROL × 1 SO4 SULFATE ION × 11 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7；291 K；0.2M Ammonium Chloride, 0.1M MES, 20% PEG 4000
|
Resolution 2.40 Å R-free 0.262 |
| 5JDD Crystal structure of I9-I11 tandem from titin (P212121) Deposited 2016-04-16 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
2795–3053（259 aa）
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；292 K；0.1 M Bis-Tris Propane pH 8.5, 20% [w/v] PEG 3350, 0.2 M sodium acetate
|
Resolution 1.53 Å R-free 0.197 |
| 5JDE Crystal structure of I9-I11 tandem from titin (P1) Deposited 2016-04-16 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
2795–3053（259 aa）
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；292 K；0.1 M Tris HCl pH 8.5, 30% [w/v] PEG 4000, 0.2 M MgCl2
|
Resolution 1.90 Å R-free 0.219 |
| 5JDE Crystal structure of I9-I11 tandem from titin (P1) Deposited 2016-04-16 | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain B
2795–3053（259 aa）
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；292 K；0.1 M Tris HCl pH 8.5, 30% [w/v] PEG 4000, 0.2 M MgCl2
|
Resolution 1.90 Å R-free 0.219 |
| 5JDJ Crystal structure of domain I10 from titin in space group P212121 Deposited 2016-04-16 | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
2880–2967（88 aa）
Chain L
2880–2967（88 aa）
|
Not recorded | CA CALCIUM ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；292 K；0.2 M CaCl2, 0.1 M Tris pH 7.5, 25% [w/v] PEG 8000
|
Resolution 1.74 Å R-free 0.224 |
| 5JDJ Crystal structure of domain I10 from titin in space group P212121 Deposited 2016-04-16 | Assembly 2 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain B
2880–2967（88 aa）
Chain P
2880–2967（88 aa）
|
Not recorded | CA CALCIUM ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；292 K；0.2 M CaCl2, 0.1 M Tris pH 7.5, 25% [w/v] PEG 8000
|
Resolution 1.74 Å R-free 0.224 |
| 5JDJ Crystal structure of domain I10 from titin in space group P212121 Deposited 2016-04-16 | Assembly 3 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain C
2880–2967（88 aa）
Chain M
2880–2967（88 aa）
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；292 K；0.2 M CaCl2, 0.1 M Tris pH 7.5, 25% [w/v] PEG 8000
|
Resolution 1.74 Å R-free 0.224 |
| 5JDJ Crystal structure of domain I10 from titin in space group P212121 Deposited 2016-04-16 | Assembly 4 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain D
2880–2967（88 aa）
Chain E
2880–2967（88 aa）
|
Not recorded | CA CALCIUM ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；292 K；0.2 M CaCl2, 0.1 M Tris pH 7.5, 25% [w/v] PEG 8000
|
Resolution 1.74 Å R-free 0.224 |
| 5JDJ Crystal structure of domain I10 from titin in space group P212121 Deposited 2016-04-16 | Assembly 5 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain F
2880–2967（88 aa）
Chain N
2880–2967（88 aa）
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；292 K；0.2 M CaCl2, 0.1 M Tris pH 7.5, 25% [w/v] PEG 8000
|
Resolution 1.74 Å R-free 0.224 |
| 5JDJ Crystal structure of domain I10 from titin in space group P212121 Deposited 2016-04-16 | Assembly 6 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain G
2880–2967（88 aa）
Chain J
2880–2967（88 aa）
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；292 K；0.2 M CaCl2, 0.1 M Tris pH 7.5, 25% [w/v] PEG 8000
|
Resolution 1.74 Å R-free 0.224 |
| 5JDJ Crystal structure of domain I10 from titin in space group P212121 Deposited 2016-04-16 | Assembly 7 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain H
2880–2967（88 aa）
Chain I
2880–2967（88 aa）
|
Not recorded | CA CALCIUM ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；292 K；0.2 M CaCl2, 0.1 M Tris pH 7.5, 25% [w/v] PEG 8000
|
Resolution 1.74 Å R-free 0.224 |
| 5JDJ Crystal structure of domain I10 from titin in space group P212121 Deposited 2016-04-16 | Assembly 8 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain K
2880–2967（88 aa）
Chain O
2880–2967（88 aa）
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；292 K；0.2 M CaCl2, 0.1 M Tris pH 7.5, 25% [w/v] PEG 8000
|
Resolution 1.74 Å R-free 0.224 |
| 5JOE Crystal structure of I81 from titin Deposited 2016-05-02 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
9582–9671（90 aa）
Fragment：I81 domain
|
Not recorded | IPA ISOPROPYL ALCOHOL × 1 EDO 1,2-ETHANEDIOL × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8.5；292.15 K；0.1M Tris pH 8.5; 30% w/v PEG 4000
|
Resolution 2.00 Å R-free 0.218 |
| 6DL4 Human Titin ZIg10 Deposited 2018-05-31 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
1821–1931（111 aa）
|
Not recorded | No recorded non-water small molecule | SOLUTION NMR |
NMR measurement conditions
pH 7.5；298 K；Ionic strength (raw mmCIF value) 50；Pressure 1
NMR sample composition
20 mM TRIS, 50 mM sodium chloride, 1 mM [U-99% 13C; U-99% 15N] Human Titin ZIg10, 90% H2O/10% D2O | 90% H2O/10% D2O
|
Resolution not provided |
| 6FWX Chimeric titin Z1Z2-Z1Z2 tandem (Z1212) functionalized with a GRGDS exogenous peptide from fibronectin Deposited 2018-03-07 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
1–196（196 aa）
Chain A
2–47（46 aa）
Chain A
52–196（145 aa）
|
Not recorded | EDO 1,2-ETHANEDIOL × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；10% [v/v] isopropanol, 100 mM Na2HPO4 pH 4.2, 200 mM LiSO4
|
Resolution 3.00 Å R-free 0.226 |
| 6FWX Chimeric titin Z1Z2-Z1Z2 tandem (Z1212) functionalized with a GRGDS exogenous peptide from fibronectin Deposited 2018-03-07 | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain B
1–196（196 aa）
Chain B
2–47（46 aa）
Chain B
52–196（145 aa）
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；10% [v/v] isopropanol, 100 mM Na2HPO4 pH 4.2, 200 mM LiSO4
|
Resolution 3.00 Å R-free 0.226 |
| 6H4L Structure of Titin M4 trigonal form Deposited 2018-07-21 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
33294–33395（102 aa）
|
Not recorded | ZN ZINC ION × 1 CL CHLORIDE ION × 3 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；293 K；MES pH 6.5
2M RbBr
|
Resolution 1.60 Å R-free 0.221 |
| 6HCI Crystal structure of titin M3 domain Deposited 2018-08-15 | Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain A
32712–32816（105 aa）
Chain B
32712–32816（105 aa）
Chain C
32712–32816（105 aa）
Chain D
32712–32816（105 aa）
|
Not recorded | SO4 SULFATE ION × 8 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8；293 K；0.2 M ammonium sulfate
23% w/v polyethylene glycol 4000
|
Resolution 2.12 Å R-free 0.245 |
| 6I0Y TnaC-stalled ribosome complex with the titin I27 domain folding close to the ribosomal exit tunnel Deposited 2018-10-26 | Assembly 1 Protein–RNA Heteromer；Protein × 33 PDB declaration: 36-meric（36） Consistent with all polymers |
Chain z
14318–14406（89 aa）
|
Not recorded | MG MAGNESIUM ION × 143 ZN ZINC ION × 1 TRP TRYPTOPHAN × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.20 Å |
| 6SDB Chimeric titin Z1Z2 functionalized with a KLER exogenous peptide from decorin Deposited 2019-07-26 | Assembly 1 Insufficient information Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
3–45（43 aa）
Chain A
51–196（146 aa）
|
Not recorded | ACT ACETATE ION × 6 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；295 K；1 M NH4H2PO4 pH 4.6, 100 mM sodium acetate
|
Resolution 2.80 Å R-free 0.234 |
| 6SDB Chimeric titin Z1Z2 functionalized with a KLER exogenous peptide from decorin Deposited 2019-07-26 | Assembly 2 Insufficient information Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain B
3–45（43 aa）
Chain B
51–196（146 aa）
|
Not recorded | ACT ACETATE ION × 1 SO4 SULFATE ION × 1 EDO 1,2-ETHANEDIOL × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；295 K；1 M NH4H2PO4 pH 4.6, 100 mM sodium acetate
|
Resolution 2.80 Å R-free 0.234 |
| 6SDB Chimeric titin Z1Z2 functionalized with a KLER exogenous peptide from decorin Deposited 2019-07-26 | Assembly 3 Insufficient information Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain C
3–45（43 aa）
Chain C
51–196（146 aa）
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；295 K；1 M NH4H2PO4 pH 4.6, 100 mM sodium acetate
|
Resolution 2.80 Å R-free 0.234 |
| 6SDB Chimeric titin Z1Z2 functionalized with a KLER exogenous peptide from decorin Deposited 2019-07-26 | Assembly 4 Insufficient information Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain D
3–45（43 aa）
Chain D
51–196（146 aa）
|
Not recorded | ACT ACETATE ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；295 K；1 M NH4H2PO4 pH 4.6, 100 mM sodium acetate
|
Resolution 2.80 Å R-free 0.234 |
| 6SDB Chimeric titin Z1Z2 functionalized with a KLER exogenous peptide from decorin Deposited 2019-07-26 | Assembly 5 Insufficient information Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain E
3–45（43 aa）
Chain E
51–196（146 aa）
|
Not recorded | ACT ACETATE ION × 3 PO4 PHOSPHATE ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；295 K；1 M NH4H2PO4 pH 4.6, 100 mM sodium acetate
|
Resolution 2.80 Å R-free 0.234 |
| 6YGN Titin kinase and its flanking domains Deposited 2020-03-27 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
30596–31144（549 aa）
|
Not recorded | EDO 1,2-ETHANEDIOL × 1 GOL GLYCEROL × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；293.15 K；30% PEG 8K, 0.1 MES pH6.5
|
Resolution 2.40 Å R-free 0.228 |
| 6YGN Titin kinase and its flanking domains Deposited 2020-03-27 | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain B
30596–31144（549 aa）
|
Not recorded | EDO 1,2-ETHANEDIOL × 1 GOL GLYCEROL × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；293.15 K；30% PEG 8K, 0.1 MES pH6.5
|
Resolution 2.40 Å R-free 0.228 |
| 7AHS titin-N2A Ig81-Ig83 Deposited 2020-09-25 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
9582–9851（270 aa）
|
Not recorded | EDO 1,2-ETHANEDIOL × 5 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293.15 K；25 % w/v Polyethylene glycol 3,350, 100 mM BIS-TRIS pH 5.5, 200 mM lithium sulfate
|
Resolution 2.05 Å R-free 0.235 |
| 7AHS titin-N2A Ig81-Ig83 Deposited 2020-09-25 | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain B
9582–9851（270 aa）
|
Not recorded | EDO 1,2-ETHANEDIOL × 7 SO4 SULFATE ION × 6 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293.15 K；25 % w/v Polyethylene glycol 3,350, 100 mM BIS-TRIS pH 5.5, 200 mM lithium sulfate
|
Resolution 2.05 Å R-free 0.235 |
| 7AHS titin-N2A Ig81-Ig83 Deposited 2020-09-25 | Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain C
9582–9851（270 aa）
|
Not recorded | EDO 1,2-ETHANEDIOL × 3 SO4 SULFATE ION × 2 GOL GLYCEROL × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293.15 K；25 % w/v Polyethylene glycol 3,350, 100 mM BIS-TRIS pH 5.5, 200 mM lithium sulfate
|
Resolution 2.05 Å R-free 0.235 |
| 7AHS titin-N2A Ig81-Ig83 Deposited 2020-09-25 | Assembly 4 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain D
9582–9851（270 aa）
|
Not recorded | EDO 1,2-ETHANEDIOL × 4 SO4 SULFATE ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293.15 K；25 % w/v Polyethylene glycol 3,350, 100 mM BIS-TRIS pH 5.5, 200 mM lithium sulfate
|
Resolution 2.05 Å R-free 0.235 |
| 7NIP titin N2A unique sequence (UN2A) core Deposited 2021-02-13 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
8578–8617（40 aa）
|
Not recorded | No recorded non-water small molecule | SOLUTION NMR |
NMR measurement conditions
pH 7.5；298 K；Ionic strength (raw mmCIF value) 100；Pressure 101300
NMR sample composition
30 mM sodium phosphate, 100 mM sodium chloride, 95% H2O/5% D2O | 95% H2O/5% D2O
|
Resolution not provided |
| 8BNQ Crystal structure of the FnIII-tandem A84-A86 from the A-band of titin Deposited 2022-11-14 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
23562–23862（301 aa）
|
Not recorded | EDO 1,2-ETHANEDIOL × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；277.15 K；5% [v/v] polyethylene glycol 400 and 40% [v/v] 2-methyl-2,4-pentanediol (MPD)
|
Resolution 2.30 Å R-free 0.258 |
| 8BNQ Crystal structure of the FnIII-tandem A84-A86 from the A-band of titin Deposited 2022-11-14 | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain B
23562–23862（301 aa）
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；277.15 K；5% [v/v] polyethylene glycol 400 and 40% [v/v] 2-methyl-2,4-pentanediol (MPD)
|
Resolution 2.30 Å R-free 0.258 |
| 8BVO Titin I110-I111 FnIII tandem from the MIR region (I/A5-I/A6) Deposited 2022-12-05 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
14415–14609（195 aa）
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；291.15 K；24% (w/v) PEG 1500, 20% (w/v) glycerol
|
Resolution 2.55 Å R-free 0.287 |
| 8BW6 Titin FnIII-domain I110 (I/A6) from the MIR region Deposited 2022-12-06 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
14415–14509（95 aa）
|
Not recorded | EDO 1,2-ETHANEDIOL × 7 PEG DI(HYDROXYETHYL)ETHER × 1 CA CALCIUM ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；291.15 K；0.1M Tris pH8 30% PEG 300
|
Resolution 1.95 Å R-free 0.254 |
| 8BXR Titin FnIII-domain I109-I111 (I/A4-A/A6) from the MIR region Deposited 2022-12-09 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
14319–14615（297 aa）
|
Not recorded | EDO 1,2-ETHANEDIOL × 6 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；291.15 K；0.1M MES pH 6, 10% [w/v] PEG 6000
|
Resolution 2.70 Å R-free 0.271 |
| 8G4L Cryo-EM structure of the human cardiac myosin filament Deposited 2023-02-10 | Assembly 1 Protein heterocomplex Heteromer；Protein × 123 PDB declaration: 123-meric（123） Consistent with protein count |
Chain am
22784–23867（1084 aa）
Chain an
22784–23867（1084 aa）
Chain bm
22784–23867（1084 aa）
Chain bn
22784–23867（1084 aa）
Chain m
22784–23867（1084 aa）
Chain n
22784–23867（1084 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 6.8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 6.40 Å |
| 8OIY Crystal structure of the R15908H missense variant of titin domain Fn3-3 Deposited 2023-03-23 | Assembly 1 Protein homooligomer Homooligomer；Protein × 6 PDB declaration: hexameric（6） Consistent with protein count |
Chain A
14217–14319（103 aa）
Chain B
14217–14319（103 aa）
Chain C
14217–14319（103 aa）
Chain D
14217–14319（103 aa）
Chain E
14217–14319（103 aa）
Chain F
14217–14319（103 aa）
|
Mutation：R15908H Mutation：R15908H Mutation：R15908H Mutation：R15908H Mutation：R15908H Mutation：R15908H | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；293 K；10mM Tris-HCl PH 7.5
NaCl
0.5mM DTT
0.1M sodium acetate pH 4.5
0.2M Lithium sulphate
50% v/v PEG 400
|
Resolution 2.10 Å R-free 0.257 |
| 8OMW Crystal structure of the titin domain Fn3-20 Deposited 2023-03-31 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
16525–16630（106 aa）
|
Not recorded | CL CHLORIDE ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；10mM Tris-HCl pH 7.5
50mM NaCl
0.5mM DTT
0.1M TBG pH 9.0
25% w/v PEG 1500
|
Resolution 1.05 Å R-free 0.177 |
| 8OQ9 Crystal structure of the titin domain Fn3-56 Deposited 2023-04-11 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
21497–21598（102 aa）
|
Not recorded | ZN ZINC ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；10mM Tris-HCl pH 7.5
50mM NaCl
0.5mM DTT
0.1M MES
10mM ZnCl2
20% (w/v) PEG6000
|
Resolution 1.65 Å R-free 0.192 |
| 8OQ9 Crystal structure of the titin domain Fn3-56 Deposited 2023-04-11 | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain B
21497–21598（102 aa）
|
Not recorded | ZN ZINC ION × 3 CL CHLORIDE ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；10mM Tris-HCl pH 7.5
50mM NaCl
0.5mM DTT
0.1M MES
10mM ZnCl2
20% (w/v) PEG6000
|
Resolution 1.65 Å R-free 0.192 |
| 8OQ9 Crystal structure of the titin domain Fn3-56 Deposited 2023-04-11 | Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain C
21497–21598（102 aa）
|
Not recorded | ZN ZINC ION × 1 CL CHLORIDE ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；10mM Tris-HCl pH 7.5
50mM NaCl
0.5mM DTT
0.1M MES
10mM ZnCl2
20% (w/v) PEG6000
|
Resolution 1.65 Å R-free 0.192 |
| 8ORL Crystal structure of the S23226G missense variant of titin domain Fn3-56 Deposited 2023-04-14 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
21497–21598（102 aa）
|
Not recorded | ZN ZINC ION × 1 CL CHLORIDE ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；10mM Tris-HCl pH 7.5
50mM NaCl
0.5mM DTT
0.1M MES
10mM ZnCl2
20% (w/v) PEG6000
|
Resolution 1.43 Å R-free 0.194 |
| 8ORL Crystal structure of the S23226G missense variant of titin domain Fn3-56 Deposited 2023-04-14 | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain B
21497–21598（102 aa）
|
Not recorded | ZN ZINC ION × 3 CL CHLORIDE ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；10mM Tris-HCl pH 7.5
50mM NaCl
0.5mM DTT
0.1M MES
10mM ZnCl2
20% (w/v) PEG6000
|
Resolution 1.43 Å R-free 0.194 |
| 8ORL Crystal structure of the S23226G missense variant of titin domain Fn3-56 Deposited 2023-04-14 | Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain C
21497–21598（102 aa）
|
Not recorded | ZN ZINC ION × 1 CL CHLORIDE ION × 3 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；10mM Tris-HCl pH 7.5
50mM NaCl
0.5mM DTT
0.1M MES
10mM ZnCl2
20% (w/v) PEG6000
|
Resolution 1.43 Å R-free 0.194 |
| 8OS3 Crystal structure of the titin domain Fn3-11 Deposited 2023-04-17 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
15210–15313（104 aa）
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；30mM HEPES pH 7.5
50mM NaCl
0.5mM DTT
100mM sodium acetate pH 4.5
200mM Lithium sulphate
50% v/v PEG 400
|
Resolution 1.68 Å R-free 0.208 |
| 8OSD Crystal structure of the titin domain Fn3-49 Deposited 2023-04-18 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
20511–20616（106 aa）
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；30mM HEPES pH 7.5
150mM NaCl
1mM DTT
100mM Potassium thiocyanate
30% w/v PEG MME2000
|
Resolution 1.70 Å R-free 0.260 |
| 8OSD Crystal structure of the titin domain Fn3-49 Deposited 2023-04-18 | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain B
20511–20616（106 aa）
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；30mM HEPES pH 7.5
150mM NaCl
1mM DTT
100mM Potassium thiocyanate
30% w/v PEG MME2000
|
Resolution 1.70 Å R-free 0.260 |
| 8OT5 Crystal structure of the titin domain Fn3-85 Deposited 2023-04-20 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
25430–25531（102 aa）
|
Not recorded | NA SODIUM ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；30mM HEPES pH 7.5
150mM NaCl
1mM DTT
100mM Bis-Tris propane pH 6.5
200mM sodium iodide
20% w/v PEG3350
|
Resolution 1.56 Å R-free 0.191 |
| 8OT5 Crystal structure of the titin domain Fn3-85 Deposited 2023-04-20 | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain B
25430–25531（102 aa）
|
Not recorded | NA SODIUM ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；30mM HEPES pH 7.5
150mM NaCl
1mM DTT
100mM Bis-Tris propane pH 6.5
200mM sodium iodide
20% w/v PEG3350
|
Resolution 1.56 Å R-free 0.191 |
| 8OT5 Crystal structure of the titin domain Fn3-85 Deposited 2023-04-20 | Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain C
25430–25531（102 aa）
|
Not recorded | NA SODIUM ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；30mM HEPES pH 7.5
150mM NaCl
1mM DTT
100mM Bis-Tris propane pH 6.5
200mM sodium iodide
20% w/v PEG3350
|
Resolution 1.56 Å R-free 0.191 |
| 8OT5 Crystal structure of the titin domain Fn3-85 Deposited 2023-04-20 | Assembly 4 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain D
25430–25531（102 aa）
|
Not recorded | NA SODIUM ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；30mM HEPES pH 7.5
150mM NaCl
1mM DTT
100mM Bis-Tris propane pH 6.5
200mM sodium iodide
20% w/v PEG3350
|
Resolution 1.56 Å R-free 0.191 |
| 8OT5 Crystal structure of the titin domain Fn3-85 Deposited 2023-04-20 | Assembly 5 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain E
25430–25531（102 aa）
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；30mM HEPES pH 7.5
150mM NaCl
1mM DTT
100mM Bis-Tris propane pH 6.5
200mM sodium iodide
20% w/v PEG3350
|
Resolution 1.56 Å R-free 0.191 |
| 8OT5 Crystal structure of the titin domain Fn3-85 Deposited 2023-04-20 | Assembly 6 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain F
25430–25531（102 aa）
|
Not recorded | CL CHLORIDE ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；30mM HEPES pH 7.5
150mM NaCl
1mM DTT
100mM Bis-Tris propane pH 6.5
200mM sodium iodide
20% w/v PEG3350
|
Resolution 1.56 Å R-free 0.191 |
| 8OTY Crystal structure of the titin domain Fn3-90 Deposited 2023-04-21 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
26124–26224（101 aa）
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；30mM HEPES pH 7.5
150mM NaCl
1mM DTT
100mM Sodium acetate
200mM CaCl
20% w/v PEG6000
|
Resolution 1.90 Å R-free 0.251 |
| 8OTY Crystal structure of the titin domain Fn3-90 Deposited 2023-04-21 | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain B
26124–26224（101 aa）
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；30mM HEPES pH 7.5
150mM NaCl
1mM DTT
100mM Sodium acetate
200mM CaCl
20% w/v PEG6000
|
Resolution 1.90 Å R-free 0.251 |
| 8OTY Crystal structure of the titin domain Fn3-90 Deposited 2023-04-21 | Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain C
26124–26224（101 aa）
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；30mM HEPES pH 7.5
150mM NaCl
1mM DTT
100mM Sodium acetate
200mM CaCl
20% w/v PEG6000
|
Resolution 1.90 Å R-free 0.251 |
| 8OTY Crystal structure of the titin domain Fn3-90 Deposited 2023-04-21 | Assembly 4 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain D
26124–26224（101 aa）
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；30mM HEPES pH 7.5
150mM NaCl
1mM DTT
100mM Sodium acetate
200mM CaCl
20% w/v PEG6000
|
Resolution 1.90 Å R-free 0.251 |
| 8OVU Human titin immunoglobulin-like 21 domain Deposited 2023-04-26 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
3500–3598（99 aa）
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.5；293 K；0.06 M divalent ions (CaCl2, MgCl2), 0.1 M sodium HEPES, pH 7.5, 50% v/v Polyethylene glycol monomethyl ether (PEGMME) 550, PEG 20K from Morpheus HT-96 screening plate (Molecular Dimensions)
|
Resolution 1.95 Å R-free 0.239 |
| 8OVU Human titin immunoglobulin-like 21 domain Deposited 2023-04-26 | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain B
3500–3598（99 aa）
|
Not recorded | MG MAGNESIUM ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.5；293 K；0.06 M divalent ions (CaCl2, MgCl2), 0.1 M sodium HEPES, pH 7.5, 50% v/v Polyethylene glycol monomethyl ether (PEGMME) 550, PEG 20K from Morpheus HT-96 screening plate (Molecular Dimensions)
|
Resolution 1.95 Å R-free 0.239 |
| 8P35 Mutant human titin immunoglobulin-like 21 domain - C3575S Deposited 2023-05-17 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
3500–3598（99 aa）
|
Mutation：C3575S | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.5；293 K；0.1 M HEPES sodium salt, pH 7.5, 25 %(w/v) PEG 2000 MME
|
Resolution 2.20 Å R-free 0.250 |
| 8P35 Mutant human titin immunoglobulin-like 21 domain - C3575S Deposited 2023-05-17 | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain B
3500–3598（99 aa）
|
Mutation：C3575S | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.5；293 K；0.1 M HEPES sodium salt, pH 7.5, 25 %(w/v) PEG 2000 MME
|
Resolution 2.20 Å R-free 0.250 |
| 8P35 Mutant human titin immunoglobulin-like 21 domain - C3575S Deposited 2023-05-17 | Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain C
3500–3598（99 aa）
|
Mutation：C3575S | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.5；293 K；0.1 M HEPES sodium salt, pH 7.5, 25 %(w/v) PEG 2000 MME
|
Resolution 2.20 Å R-free 0.250 |
| 8P35 Mutant human titin immunoglobulin-like 21 domain - C3575S Deposited 2023-05-17 | Assembly 4 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain D
3500–3598（99 aa）
|
Mutation：C3575S | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.5；293 K；0.1 M HEPES sodium salt, pH 7.5, 25 %(w/v) PEG 2000 MME
|
Resolution 2.20 Å R-free 0.250 |
| 8P35 Mutant human titin immunoglobulin-like 21 domain - C3575S Deposited 2023-05-17 | Assembly 5 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain E
3500–3598（99 aa）
|
Mutation：C3575S | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.5；293 K；0.1 M HEPES sodium salt, pH 7.5, 25 %(w/v) PEG 2000 MME
|
Resolution 2.20 Å R-free 0.250 |
| 8P35 Mutant human titin immunoglobulin-like 21 domain - C3575S Deposited 2023-05-17 | Assembly 6 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain F
3500–3598（99 aa）
|
Mutation：C3575S | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.5；293 K；0.1 M HEPES sodium salt, pH 7.5, 25 %(w/v) PEG 2000 MME
|
Resolution 2.20 Å R-free 0.250 |