Current Protein Identity:Q99278
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Difference tags compare only the current result set; every original PDB and assembly record remains separate.
Related-Structure Differences
Each row represents one biological assembly in one PDB entry; multiple monomers of the same protein are listed separately.
| PDB Entry | Assembly / Oligomeric State | Construct | Mutations and Modifications | Ligands, Ions and Non-polymers | Experimental Method | Experimental Conditions | Structure Quality |
|---|---|---|---|---|---|---|---|
| 3R84 Structure of the Mediator head subcomplex Med11/22 Deposited 2011-03-23 | Assembly 1 Protein heterocomplex Heteromer;Protein × 2 PDB declaration: dimeric(2) Consistent with protein count |
Chain A
21–105(85 aa)
Fragment:Residues 21-105
|
Non-standard monomer:Yes (specific site not provided by mmCIF) | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP;pH 6.5;291 K;100 mM MES pH 6, 5.5% PEG 6000 and 100 mM MgCl2, VAPOR DIFFUSION, HANGING DROP, temperature 291K
|
Resolution 2.05 Å R-free 0.208 |
| 3R84 Structure of the Mediator head subcomplex Med11/22 Deposited 2011-03-23 | Assembly 10 Protein heterocomplex Heteromer;Protein × 2 PDB declaration: dimeric(2) Consistent with protein count |
Chain S
21–105(85 aa)
Fragment:Residues 21-105
|
Non-standard monomer:Yes (specific site not provided by mmCIF) | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP;pH 6.5;291 K;100 mM MES pH 6, 5.5% PEG 6000 and 100 mM MgCl2, VAPOR DIFFUSION, HANGING DROP, temperature 291K
|
Resolution 2.05 Å R-free 0.208 |
| 3R84 Structure of the Mediator head subcomplex Med11/22 Deposited 2011-03-23 | Assembly 11 Protein heterocomplex Heteromer;Protein × 2 PDB declaration: dimeric(2) Consistent with protein count |
Chain U
21–105(85 aa)
Fragment:Residues 21-105
|
Non-standard monomer:Yes (specific site not provided by mmCIF) | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP;pH 6.5;291 K;100 mM MES pH 6, 5.5% PEG 6000 and 100 mM MgCl2, VAPOR DIFFUSION, HANGING DROP, temperature 291K
|
Resolution 2.05 Å R-free 0.208 |
| 3R84 Structure of the Mediator head subcomplex Med11/22 Deposited 2011-03-23 | Assembly 12 Protein heterocomplex Heteromer;Protein × 2 PDB declaration: dimeric(2) Consistent with protein count |
Chain W
21–105(85 aa)
Fragment:Residues 21-105
|
Non-standard monomer:Yes (specific site not provided by mmCIF) | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP;pH 6.5;291 K;100 mM MES pH 6, 5.5% PEG 6000 and 100 mM MgCl2, VAPOR DIFFUSION, HANGING DROP, temperature 291K
|
Resolution 2.05 Å R-free 0.208 |
| 3R84 Structure of the Mediator head subcomplex Med11/22 Deposited 2011-03-23 | Assembly 13 Protein heterocomplex Heteromer;Protein × 4 PDB declaration: tetrameric(4) Consistent with protein count |
Chain A
21–105(85 aa)
Fragment:Residues 21-105
Chain C
21–105(85 aa)
Fragment:Residues 21-105
|
Non-standard monomer:Yes (specific site not provided by mmCIF) Non-standard monomer:Yes (specific site not provided by mmCIF) | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP;pH 6.5;291 K;100 mM MES pH 6, 5.5% PEG 6000 and 100 mM MgCl2, VAPOR DIFFUSION, HANGING DROP, temperature 291K
|
Resolution 2.05 Å R-free 0.208 |
| 3R84 Structure of the Mediator head subcomplex Med11/22 Deposited 2011-03-23 | Assembly 14 Protein heterocomplex Heteromer;Protein × 4 PDB declaration: tetrameric(4) Consistent with protein count |
Chain E
21–105(85 aa)
Fragment:Residues 21-105
Chain G
21–105(85 aa)
Fragment:Residues 21-105
|
Non-standard monomer:Yes (specific site not provided by mmCIF) Non-standard monomer:Yes (specific site not provided by mmCIF) | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP;pH 6.5;291 K;100 mM MES pH 6, 5.5% PEG 6000 and 100 mM MgCl2, VAPOR DIFFUSION, HANGING DROP, temperature 291K
|
Resolution 2.05 Å R-free 0.208 |
| 3R84 Structure of the Mediator head subcomplex Med11/22 Deposited 2011-03-23 | Assembly 15 Protein heterocomplex Heteromer;Protein × 4 PDB declaration: tetrameric(4) Consistent with protein count |
Chain I
21–105(85 aa)
Fragment:Residues 21-105
Chain K
21–105(85 aa)
Fragment:Residues 21-105
|
Non-standard monomer:Yes (specific site not provided by mmCIF) Non-standard monomer:Yes (specific site not provided by mmCIF) | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP;pH 6.5;291 K;100 mM MES pH 6, 5.5% PEG 6000 and 100 mM MgCl2, VAPOR DIFFUSION, HANGING DROP, temperature 291K
|
Resolution 2.05 Å R-free 0.208 |
| 3R84 Structure of the Mediator head subcomplex Med11/22 Deposited 2011-03-23 | Assembly 16 Protein heterocomplex Heteromer;Protein × 4 PDB declaration: tetrameric(4) Consistent with protein count |
Chain M
21–105(85 aa)
Fragment:Residues 21-105
Chain O
21–105(85 aa)
Fragment:Residues 21-105
|
Non-standard monomer:Yes (specific site not provided by mmCIF) Non-standard monomer:Yes (specific site not provided by mmCIF) | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP;pH 6.5;291 K;100 mM MES pH 6, 5.5% PEG 6000 and 100 mM MgCl2, VAPOR DIFFUSION, HANGING DROP, temperature 291K
|
Resolution 2.05 Å R-free 0.208 |
| 3R84 Structure of the Mediator head subcomplex Med11/22 Deposited 2011-03-23 | Assembly 17 Protein heterocomplex Heteromer;Protein × 4 PDB declaration: tetrameric(4) Consistent with protein count |
Chain Q
21–105(85 aa)
Fragment:Residues 21-105
Chain S
21–105(85 aa)
Fragment:Residues 21-105
|
Non-standard monomer:Yes (specific site not provided by mmCIF) Non-standard monomer:Yes (specific site not provided by mmCIF) | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP;pH 6.5;291 K;100 mM MES pH 6, 5.5% PEG 6000 and 100 mM MgCl2, VAPOR DIFFUSION, HANGING DROP, temperature 291K
|
Resolution 2.05 Å R-free 0.208 |
| 3R84 Structure of the Mediator head subcomplex Med11/22 Deposited 2011-03-23 | Assembly 18 Protein heterocomplex Heteromer;Protein × 4 PDB declaration: tetrameric(4) Consistent with protein count |
Chain U
21–105(85 aa)
Fragment:Residues 21-105
Chain W
21–105(85 aa)
Fragment:Residues 21-105
|
Non-standard monomer:Yes (specific site not provided by mmCIF) Non-standard monomer:Yes (specific site not provided by mmCIF) | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP;pH 6.5;291 K;100 mM MES pH 6, 5.5% PEG 6000 and 100 mM MgCl2, VAPOR DIFFUSION, HANGING DROP, temperature 291K
|
Resolution 2.05 Å R-free 0.208 |
| 3R84 Structure of the Mediator head subcomplex Med11/22 Deposited 2011-03-23 | Assembly 19 Protein heterocomplex Heteromer;Protein × 24 PDB declaration: 24-meric(24) Consistent with protein count |
Chain A
21–105(85 aa)
Fragment:Residues 21-105
Chain C
21–105(85 aa)
Fragment:Residues 21-105
Chain E
21–105(85 aa)
Fragment:Residues 21-105
Chain G
21–105(85 aa)
Fragment:Residues 21-105
Chain I
21–105(85 aa)
Fragment:Residues 21-105
Chain K
21–105(85 aa)
Fragment:Residues 21-105
Chain M
21–105(85 aa)
Fragment:Residues 21-105
Chain O
21–105(85 aa)
Fragment:Residues 21-105
Chain Q
21–105(85 aa)
Fragment:Residues 21-105
Chain S
21–105(85 aa)
Fragment:Residues 21-105
Chain U
21–105(85 aa)
Fragment:Residues 21-105
Chain W
21–105(85 aa)
Fragment:Residues 21-105
|
Non-standard monomer:Yes (specific site not provided by mmCIF) Non-standard monomer:Yes (specific site not provided by mmCIF) Non-standard monomer:Yes (specific site not provided by mmCIF) Non-standard monomer:Yes (specific site not provided by mmCIF) Non-standard monomer:Yes (specific site not provided by mmCIF) Non-standard monomer:Yes (specific site not provided by mmCIF) Non-standard monomer:Yes (specific site not provided by mmCIF) Non-standard monomer:Yes (specific site not provided by mmCIF) Non-standard monomer:Yes (specific site not provided by mmCIF) Non-standard monomer:Yes (specific site not provided by mmCIF) Non-standard monomer:Yes (specific site not provided by mmCIF) Non-standard monomer:Yes (specific site not provided by mmCIF) | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP;pH 6.5;291 K;100 mM MES pH 6, 5.5% PEG 6000 and 100 mM MgCl2, VAPOR DIFFUSION, HANGING DROP, temperature 291K
|
Resolution 2.05 Å R-free 0.208 |
| 3R84 Structure of the Mediator head subcomplex Med11/22 Deposited 2011-03-23 | Assembly 2 Protein heterocomplex Heteromer;Protein × 2 PDB declaration: dimeric(2) Consistent with protein count |
Chain C
21–105(85 aa)
Fragment:Residues 21-105
|
Non-standard monomer:Yes (specific site not provided by mmCIF) | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP;pH 6.5;291 K;100 mM MES pH 6, 5.5% PEG 6000 and 100 mM MgCl2, VAPOR DIFFUSION, HANGING DROP, temperature 291K
|
Resolution 2.05 Å R-free 0.208 |
| 3R84 Structure of the Mediator head subcomplex Med11/22 Deposited 2011-03-23 | Assembly 3 Protein heterocomplex Heteromer;Protein × 2 PDB declaration: dimeric(2) Consistent with protein count |
Chain E
21–105(85 aa)
Fragment:Residues 21-105
|
Non-standard monomer:Yes (specific site not provided by mmCIF) | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP;pH 6.5;291 K;100 mM MES pH 6, 5.5% PEG 6000 and 100 mM MgCl2, VAPOR DIFFUSION, HANGING DROP, temperature 291K
|
Resolution 2.05 Å R-free 0.208 |
| 3R84 Structure of the Mediator head subcomplex Med11/22 Deposited 2011-03-23 | Assembly 4 Protein heterocomplex Heteromer;Protein × 2 PDB declaration: dimeric(2) Consistent with protein count |
Chain G
21–105(85 aa)
Fragment:Residues 21-105
|
Non-standard monomer:Yes (specific site not provided by mmCIF) | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP;pH 6.5;291 K;100 mM MES pH 6, 5.5% PEG 6000 and 100 mM MgCl2, VAPOR DIFFUSION, HANGING DROP, temperature 291K
|
Resolution 2.05 Å R-free 0.208 |
| 3R84 Structure of the Mediator head subcomplex Med11/22 Deposited 2011-03-23 | Assembly 5 Protein heterocomplex Heteromer;Protein × 2 PDB declaration: dimeric(2) Consistent with protein count |
Chain I
21–105(85 aa)
Fragment:Residues 21-105
|
Non-standard monomer:Yes (specific site not provided by mmCIF) | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP;pH 6.5;291 K;100 mM MES pH 6, 5.5% PEG 6000 and 100 mM MgCl2, VAPOR DIFFUSION, HANGING DROP, temperature 291K
|
Resolution 2.05 Å R-free 0.208 |
| 3R84 Structure of the Mediator head subcomplex Med11/22 Deposited 2011-03-23 | Assembly 6 Protein heterocomplex Heteromer;Protein × 2 PDB declaration: dimeric(2) Consistent with protein count |
Chain K
21–105(85 aa)
Fragment:Residues 21-105
|
Non-standard monomer:Yes (specific site not provided by mmCIF) | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP;pH 6.5;291 K;100 mM MES pH 6, 5.5% PEG 6000 and 100 mM MgCl2, VAPOR DIFFUSION, HANGING DROP, temperature 291K
|
Resolution 2.05 Å R-free 0.208 |
| 3R84 Structure of the Mediator head subcomplex Med11/22 Deposited 2011-03-23 | Assembly 7 Protein heterocomplex Heteromer;Protein × 2 PDB declaration: dimeric(2) Consistent with protein count |
Chain M
21–105(85 aa)
Fragment:Residues 21-105
|
Non-standard monomer:Yes (specific site not provided by mmCIF) | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP;pH 6.5;291 K;100 mM MES pH 6, 5.5% PEG 6000 and 100 mM MgCl2, VAPOR DIFFUSION, HANGING DROP, temperature 291K
|
Resolution 2.05 Å R-free 0.208 |
| 3R84 Structure of the Mediator head subcomplex Med11/22 Deposited 2011-03-23 | Assembly 8 Protein heterocomplex Heteromer;Protein × 2 PDB declaration: dimeric(2) Consistent with protein count |
Chain O
21–105(85 aa)
Fragment:Residues 21-105
|
Non-standard monomer:Yes (specific site not provided by mmCIF) | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP;pH 6.5;291 K;100 mM MES pH 6, 5.5% PEG 6000 and 100 mM MgCl2, VAPOR DIFFUSION, HANGING DROP, temperature 291K
|
Resolution 2.05 Å R-free 0.208 |
| 3R84 Structure of the Mediator head subcomplex Med11/22 Deposited 2011-03-23 | Assembly 9 Protein heterocomplex Heteromer;Protein × 2 PDB declaration: dimeric(2) Consistent with protein count |
Chain Q
21–105(85 aa)
Fragment:Residues 21-105
|
Non-standard monomer:Yes (specific site not provided by mmCIF) | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP;pH 6.5;291 K;100 mM MES pH 6, 5.5% PEG 6000 and 100 mM MgCl2, VAPOR DIFFUSION, HANGING DROP, temperature 291K
|
Resolution 2.05 Å R-free 0.208 |
| 3RJ1 Architecture of the Mediator Head module Deposited 2011-04-14 | Assembly 1 Protein heterocomplex Heteromer;Protein × 7 PDB declaration: heptameric(7) Consistent with protein count |
Chain A
1–131(131 aa)
|
Mutation:T16G, M17S Non-standard monomer:Yes (specific site not provided by mmCIF) | SE SELENIUM ATOM × 30 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP;pH 7.6;293 K;0.1 M Tris-HCl (pH 7.6) containing 10-12.5% (w/v) PEG-6K, and 0.4 M (NH4)2SO4, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 4.30 Å R-free 0.373 |
| 3RJ1 Architecture of the Mediator Head module Deposited 2011-04-14 | Assembly 2 Protein heterocomplex Heteromer;Protein × 7 PDB declaration: heptameric(7) Consistent with protein count |
Chain H
1–131(131 aa)
|
Mutation:T16G, M17S Non-standard monomer:Yes (specific site not provided by mmCIF) | SE SELENIUM ATOM × 36 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP;pH 7.6;293 K;0.1 M Tris-HCl (pH 7.6) containing 10-12.5% (w/v) PEG-6K, and 0.4 M (NH4)2SO4, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 4.30 Å R-free 0.373 |
| 3RJ1 Architecture of the Mediator Head module Deposited 2011-04-14 | Assembly 3 Protein heterocomplex Heteromer;Protein × 7 PDB declaration: heptameric(7) Consistent with protein count |
Chain O
1–131(131 aa)
|
Mutation:T16G, M17S Non-standard monomer:Yes (specific site not provided by mmCIF) | SE SELENIUM ATOM × 32 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP;pH 7.6;293 K;0.1 M Tris-HCl (pH 7.6) containing 10-12.5% (w/v) PEG-6K, and 0.4 M (NH4)2SO4, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 4.30 Å R-free 0.373 |
| 4GWP Structure of the Mediator Head Module from S. cerevisiae Deposited 2012-09-03 | Assembly 1 Protein heterocomplex Heteromer;Protein × 7 PDB declaration: heptameric(7) Consistent with protein count |
Chain A
1–115(115 aa)
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP;pH 7;291.2 K;275mM ammonium citrate tribasic pH 7.0, 10% w/v PEG 3350, 2mM DTT, VAPOR DIFFUSION, HANGING DROP, temperature 291.2K
|
Resolution 4.20 Å R-free 0.357 |
| 4GWQ Structure of the Mediator Head Module from S. cerevisiae in complex with the carboxy-terminal domain (CTD) of RNA Polymerase II Rpb1 subunit Deposited 2012-09-03 | Assembly 1 Protein heterocomplex Heteromer;Protein × 8 PDB declaration: octameric(8) Consistent with protein count |
Chain A
1–115(115 aa)
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 7;291.2 K;275mM ammonium citrate tribasic pH 7.0, 10% w/v PEG 3350, 2mM DTT, VAPOR DIFFUSION, HANGING DROP, temperature 291.2K
|
Resolution 4.50 Å R-free 0.345 |
| 4H62 Structure of the Saccharomyces cerevisiae Mediator subcomplex Med17C/Med11C/Med22C Deposited 2012-09-19 | Assembly 1 Protein heterocomplex Heteromer;Protein × 3 PDB declaration: trimeric(3) Consistent with protein count |
Chain K
84–115(32 aa)
Fragment:C-terminal region (Unp residues 84-115)
|
Non-standard monomer:Yes (specific site not provided by mmCIF) | MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP;pH 6;293 K;100 mM MES, 4 M ammonium acetate, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 3.00 Å R-free 0.229 |
| 4V1O Architecture of the RNA polymerase II-Mediator core transcription initiation complex Deposited 2014-09-29 | Assembly 1 Other combination Heteromer;Protein × 23 PDB declaration: 26-meric(26) Consistent with all polymers |
Chain V
1–115(115 aa)
|
Not recorded | ZN ZINC ION × 9 MG MAGNESIUM ION × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
25 MM HEPES-KOH PH 7.5, 180 MM POTASSIUM ACETATE, 5 % GLYCEROL, 5 MM DTT;pH 7.5;25 MM HEPES-KOH PH 7.5, 180 MM POTASSIUM ACETATE, 5 % GLYCEROL, 5 MM DTT
cryo-EM vitrification conditions
Cryogen ETHANE;GRIDS WERE GLOW- DISCHARGED FOR 20 S BEFORE DEPOSITION OF 4 MICROLITERS SAMPLE AND INCUBATED FOR 30 S. GRIDS WERE WASHED TWICE WITH 4 MICROLITERS DISTILLED WATER, BLOTTED, AND VITRIFIED BY PLUNGING INTO LIQUID ETHANE WITH A MANUAL PLUNGER.
|
Resolution 9.70 Å |
| 5OQM STRUCTURE OF YEAST TRANSCRIPTION PRE-INITIATION COMPLEX WITH TFIIH AND CORE MEDIATOR Deposited 2017-08-13 | Assembly 1 Insufficient information Heteromer;Protein × 44 PDB declaration: 46-meric(46) Consistent with all polymers |
Chain c
1–115(115 aa)
|
Not recorded | ZN ZINC ION × 16 MG MAGNESIUM ION × 1 SF4 IRON/SULFUR CLUSTER × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE;Grids were blotted for 8 s and plunge-frozen in liquid ethane.
|
Resolution 5.80 Å |
| 5SVA Mediator-RNA Polymerase II Pre-Initiation Complex Deposited 2016-08-05 | Assembly 1 Protein–DNA Heteromer;Protein × 38 PDB declaration: 40-meric(40) Consistent with all polymers |
Chain O
1–115(115 aa)
|
Not recorded | ZN ZINC ION × 8 MG MAGNESIUM ION × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 15.30 Å |
| 7UI9 Core Mediator-PICearly (Copy A) Deposited 2022-03-28 | Assembly 1 Protein heterocomplex Heteromer;Protein × 33 PDB declaration: 33-meric(33) Consistent with protein count |
Chain k
1–115(115 aa)
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.30 Å |
| 7UIF Mediator-PIC Early (Core B) Deposited 2022-03-29 | Assembly 1 Protein heterocomplex Heteromer;Protein × 33 PDB declaration: 33-meric(33) Consistent with protein count |
Chain k
1–115(115 aa)
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.60 Å |
| 7UIG Mediator-PIC Early (Mediator A) Deposited 2022-03-29 | Assembly 1 Protein heterocomplex Heteromer;Protein × 17 PDB declaration: heptadecameric(17) Consistent with protein count |
Chain k
1–115(115 aa)
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.30 Å |
| 7UIO Mediator-PIC Early (Composite Model) Deposited 2022-03-29 | Assembly 1 Protein–DNA Heteromer;Protein × 78 PDB declaration: 80-meric(80) Consistent with all polymers |
Chain Ak
1–115(115 aa)
Chain Bk
1–115(115 aa)
|
Not recorded | ZN ZINC ION × 4 THR THREONINE × 1 ALA ALANINE × 1 ASP ASPARTIC ACID × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.30 Å |