Current Protein Identity:Q99638
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Difference tags compare only the current result set; every original PDB and assembly record remains separate.
Related-Structure Differences
Each row represents one biological assembly in one PDB entry; multiple monomers of the same protein are listed separately.
| PDB Entry | Assembly / Oligomeric State | Construct | Mutations and Modifications | Ligands, Ions and Non-polymers | Experimental Method | Experimental Conditions | Structure Quality |
|---|---|---|---|---|---|---|---|
| 3A1J Crystal structure of the human Rad9-Hus1-Rad1 complex Deposited 2009-04-08 | Assembly 1 Other combination Heteromer;Protein × 3 PDB declaration: trimeric(3) Consistent with protein count |
Chain A
1–266(266 aa)
Fragment:N-terminal domain, residues 1-266
|
Non-standard monomer:Yes (specific site not provided by mmCIF) | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP;pH 6.8;296 K;15% PEG 3350, 0.18M KCl, 0.16M guanidine hydrochloride, 0.33M ZWITTERGENT 3-08, pH 6.8, VAPOR DIFFUSION, HANGING DROP, temperature 296K
|
Resolution 2.50 Å R-free 0.282 |
| 3G65 Crystal Structure of the Human Rad9-Rad1-Hus1 DNA Damage Checkpoint Complex Deposited 2009-02-06 | Assembly 1 Protein heterocomplex Heteromer;Protein × 3 PDB declaration: trimeric(3) Consistent with protein count |
Chain A
1–270(270 aa)
Fragment:UNP residues 1-270
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP;pH 8;293 K;0.1M Tris-HCl, 17% PEG 2000-Mme, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.90 Å R-free 0.272 |
| 3GGR Crystal Structure of the Human Rad9-Hus1-Rad1 complex Deposited 2009-03-02 | Assembly 1 Protein heterocomplex Heteromer;Protein × 3 PDB declaration: trimeric(3) Consistent with protein count |
Chain A
1–270(270 aa)
Fragment:truncated hRad9 (residues 1-270)
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP;pH 8;289 K;8% PEG 2000 MME, 100mM Tris-HCl,200mM Trimethylamine N-oxide dihydrate, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 289K
|
Resolution 3.20 Å R-free 0.306 |
| 6HM5 Crystal structure of TOPBP1 BRCT0,1,2 in complex with a RAD9 phosphopeptide Deposited 2018-09-12 | Assembly 1 Protein heterocomplex Heteromer;Protein × 2 PDB declaration: dimeric(2) Consistent with protein count |
Chain B
380–390(11 aa)
|
Non-standard monomer:Yes (specific site not provided by mmCIF) | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP;287.15 K;100 mM bicine/Trizma base pH 8.5, 300 mM diethyleneglycol, 300 mM triethyleneglycol, 300 mM tetraethyleneglycol, 300 mM pentaethyleneglycol, 10% w/v PEG 20000 and 20% v/v PEG MME 550
|
Resolution 2.33 Å R-free 0.241 |
| 6J8Y Crystal structure of the human RAD9-HUS1-RAD1-RHINO complex Deposited 2019-01-21 | Assembly 1 Protein heterocomplex Heteromer;Protein × 4 PDB declaration: tetrameric(4) Consistent with protein count |
Chain A
1–270(270 aa)
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP;293 K;PEG 3350, Sodium citrate, Bis-tris propane pH 7.5
|
Resolution 2.40 Å R-free 0.258 |
| 7Z6H Structure of DNA-bound human RAD17-RFC clamp loader and 9-1-1 checkpoint clamp Deposited 2022-03-11 | Assembly 1 Insufficient information Heteromer;Protein × 8 PDB declaration: decameric(10) Consistent with all polymers |
Chain A
1–391(391 aa)
|
Not recorded | AGS PHOSPHOTHIOPHOSPHORIC ACID-ADENYLATE ESTER × 5 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE-PROPANE
|
Resolution 3.59 Å |
| 8GNN Crystal structure of the human RAD9-RAD1-HUS1-RAD17 complex Deposited 2022-08-24 | Assembly 1 Protein heterocomplex Heteromer;Protein × 4 PDB declaration: tetrameric(4) Consistent with protein count |
Chain A
1–270(270 aa)
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP;pH 7.5;293 K;Bis-Tris propane pH 7.5, sodium citrate, PEG 3350
|
Resolution 2.12 Å R-free 0.263 |
| 8JZY RPA70N-RAD9 fusion Deposited 2023-07-06 | Assembly 1 Protein heterocomplex Heteromer;Protein × 2 PDB declaration: dimeric(2) Consistent with protein count |
Chain B
296–314(19 aa)
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP;277 K;PEG 3350, sodium phosphate
|
Resolution 1.50 Å R-free 0.200 |
| 8WU8 Crystal structure of the human RAD9-RAD1(F64A/M256A/F266A)-HUS1-RHINO(88-99) complex Deposited 2023-10-20 | Assembly 1 Protein heterocomplex Heteromer;Protein × 4 PDB declaration: tetrameric(4) Consistent with protein count |
Chain A
1–270(270 aa)
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP;293 K;PEG 3350, Potassium iodine
|
Resolution 2.81 Å R-free 0.267 |