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11gj

Human Argonaute2 R315V/H316A - guide10U RNA in complex with a complementary target to position 19

Method: ELECTRON MICROSCOPY D_max: 97.1 Å Quality: GOOD

SAXS Profile

P(r) Distribution

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1. Structure Basics

entry_id	11gj
deposition_date	2026-02-23
title	Human Argonaute2 R315V/H316A - guide10U RNA in complex with a complementary target to position 19
keywords	RNA binding protein, RNP, RNA, Argonaute2, Ago2, RNA BINDING PROTEIN-RNA complex; RNA BINDING PROTEIN/RNA
method	ELECTRON MICROSCOPY

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Download Data

SAXS curve (.dat) P(r) distribution (.pr.out) Structure (CIF)

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2. SAXS Parameters (CRYSOL)

rg_guinier	28.33 Å
rg_electron	28.40 Å
i0	147979000.00
molecular_weight	87751.0 kDa
excluded_volume	106050 Å³
envelope_volume	134570 Å³
shell_volume	39267 Å³
envelope_diameter	102.4 Å
shell_rg	36.00 Å
envelope_rg	28.58 Å
shape_rg	28.40 Å
total_rg	29.03 Å
total_atoms	6116
n_residues	700
n_harmonics	20
q_range	— – 0.5000 Å⁻¹
n_points	101
shell_type	directional
solvent_density	0.3340 e/Å³
contrast_shell	0.0300 e/Å³
crysol_version	4.1.3

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3. P(r) Analysis (GNOM)

dmax	97.1 Å
rg_real	28.36 Å
rg_real_error	0.84 Å
i0_real	1.4800e+08
i0_real_error	2.4410e+06
rg_reciprocal	28.35 Å
i0_reciprocal	148000000.0000
total_estimate	0.8571
solution_quality	GOOD a GOOD solution
n_peaks	2
r_peak_primary	34.7 Å
skewness	0.473
kurtosis	-0.003
angular_range	— – 0.2800 Å⁻¹
current_alpha	0.0000
highest_alpha	39030000.0000
n_real_points	57
gnom_version	4.1.3
quality_criteria	AN1: 0.000; Oscil: 0.727; Stabil: 0.999; Sysdev: 1.000; Positv: 1.000; Valcen: 0.985; Smooth: 0.974

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4. Crystallography & Experiment

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5. Entities & Polymer Info (4)

Entity 1 polymer

description	Argonaute2 R315V/H316A
formula_weight	97277.1 kDa
ec_number	3.1.26.-
mutation	R315V, H316A, S387D, S824A, S828D, S831D, S834A
src_method	man
entity_count	1

Polymer

type	polypeptide(L)
strand_id	A
atom_count	6116

MYSGAGPALAPPAPPPPIQGYAFKPPPRPDFGTSGRTIKLQANFFEMDIPKIDIYHYELDIKPEKCPRRVNREIVEHMVQHFKTQIFGDRKPVFDGRKNLYTAMPLPIGRDKVELEVTLPGEGKDRIFKVSIKWVSCVSLQALHDALSGRLPSVPFETIQALDVVMRHLPSMRYTPVGRSFFTASEGCSNPLGGGREVWFGFHQSVRPSLWKMMLNIDVSATAFYKAQPVIEFVCEVLDFKSIEEQQKPLTDSQRVKFTKEIKGLKVEITHCGQMKRKYRVCNVTRRPASHQTFPLQQESGQTVECTVAQYFKDVAKLVLRYPHLPCLQVGQEQKHTYLPLEVCNIVAGQRCIKKLTDNQTSTMIRATARSAPDRQEEISKLMRSADFNTDPYVREFGIMVKDEMTDVTGRVLQPPSILYGGRNKAIATPVQGVWDMRNKQFHTGIEIKVWAIACFAPQRQCTEVHLKSFTEQLRKISRDAGMPIQGQPCFCKYAQGADSVEPMFRHLKNTYAGLQLVVVILPGKTPVYAEVKRVGDTVLGMATQCVQMKNVQRTTPQTLSNLCLKINVKLGGVNNILLPQGRPPVFQQPVIFLGADVTHPPAGDGKKPSIAAVVGSMDAHPNRYCATVRVQQHRQEIIQDLAAMVRELLIQFYKSTRFKPTRIIFYRDGVSEGQFQQVLHHELLAIREACIKLEKDYQPGITFIVVQKRHHTRLFCTDKNERVGKSGNIPAGTTVDTKITHPTEFDFYLCSHAGIQGTSRPSHYHVLWDDNRFSSDELQILTYQLCHTYVRCTRSVSIPAPAYYAHLVAFRARYHLVDKEHDAAEGDHTDGQANGRDHQALAKAVQVHQDTLRTMYFA

Source

organism	Homo sapiens
common_name	human
ncbi_taxonomy_id	9606
host_organism	Spodoptera frugiperda

Entity 2 polymer

description	guide RNA (g10U)
formula_weight	6458.8 kDa
src_method	syn
entity_count	1

Polymer

type	polyribonucleotide
strand_id	B

UGGAGUGUGUCAAUGGUGUU

Entity 3 polymer

description	target RNA (t10A)
formula_weight	8881.4 kDa
src_method	syn
entity_count	1

Polymer

type	polyribonucleotide
strand_id	C

AAAAUUACACCAUUGACACACUCCAAAA

Entity 4 non-polymer

description	MAGNESIUM ION
formula_weight	24.3 kDa
src_method	syn
entity_count	1

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6. Citations (1)

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resolution	3.30 Å
resolution_method	—
num_particles	34598

fit	/app/data/saxs_computation/1g/11gj/11gj.fit
pddf	/app/data/pr_computation/1g/11gj/11gj.pr.out
profile	/app/data/saxs_computation/1g/11gj/11gj.dat

Human Argonaute2 R315V/H316A - guide10U RNA in complex with a complementary target to position 19

SAXS Profile

P(r) Distribution

1. Structure Basics

Download Data

2. SAXS Parameters (CRYSOL)

3. P(r) Analysis (GNOM)

4. Crystallography & Experiment

EM 3D

5. Entities & Polymer Info (4)

Entity 1 polymer

Polymer

Source

Entity 2 polymer

Polymer

Entity 3 polymer

Polymer

Entity 4 non-polymer

6. Citations (1)

7. Files & Curves (10)

Files (7)

Curves (3)