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10be

Human AGO2 bound to a miR-20a guide and a position 10-11 mismatched target

Method: ELECTRON MICROSCOPY D_max: 99.8 Å Quality: GOOD

SAXS Profile

P(r) Distribution

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1. Structure Basics

entry_id	10be
deposition_date	2026-01-09
title	Human AGO2 bound to a miR-20a guide and a position 10-11 mismatched target
keywords	Complex, RISC, RNAi, RNA BINDING PROTEIN; RNA BINDING PROTEIN
method	ELECTRON MICROSCOPY

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Download Data

SAXS curve (.dat) P(r) distribution (.pr.out) Structure (CIF)

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2. SAXS Parameters (CRYSOL)

rg_guinier	30.92 Å
rg_electron	30.90 Å
i0	157885000.00
molecular_weight	96472.0 kDa
excluded_volume	119360 Å³
envelope_volume	157350 Å³
shell_volume	42106 Å³
envelope_diameter	105.7 Å
shell_rg	38.22 Å
envelope_rg	31.05 Å
shape_rg	30.90 Å
total_rg	31.50 Å
total_atoms	6757
n_residues	811
n_harmonics	20
q_range	— – 0.5000 Å⁻¹
n_points	101
shell_type	directional
solvent_density	0.3340 e/Å³
contrast_shell	0.0300 e/Å³
crysol_version	4.1.3

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3. P(r) Analysis (GNOM)

dmax	99.8 Å
rg_real	30.90 Å
rg_real_error	0.75 Å
i0_real	1.5790e+08
i0_real_error	2.6980e+06
rg_reciprocal	30.91 Å
i0_reciprocal	157900000.0000
total_estimate	0.8921
solution_quality	GOOD a GOOD solution
n_peaks	1
r_peak_primary	35.2 Å
skewness	0.337
kurtosis	-0.386
angular_range	— – 0.2550 Å⁻¹
current_alpha	0.0000
highest_alpha	38410000.0000
n_real_points	52
gnom_version	4.1.3
quality_criteria	AN1: 0.000; Oscil: 0.901; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.992; Smooth: 0.899

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4. Crystallography & Experiment

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5. Entities & Polymer Info (3)

Entity 1 polymer

description	Guide RNA
formula_weight	7429.4 kDa
src_method	syn
entity_count	1

Polymer

type	polyribonucleotide
strand_id	B
atom_count	6757

UAAAGUGCUUAUAGUGCAGGUAG

Entity 2 polymer

description	Target RNA
formula_weight	5684.5 kDa
src_method	syn
entity_count	1

Polymer

type	polyribonucleotide
strand_id	C

CACUAACAGCACUUUAAA

Entity 3 polymer

description	Protein argonaute-2
formula_weight	98881.8 kDa
ec_number	3.1.26.-
src_method	man
entity_count	1

Polymer

type	polypeptide(L)
strand_id	A

GAMGSMDYKDDDDKMYSGAGPALAPPAPPPPIQGYAFKPPPRPDFGTSGRTIKLQANFFEMDIPKIDIYHYELDIKPEKCPRRVNREIVEHMVQHFKTQIFGDRKPVFDGRKNLYTAMPLPIGRDKVELEVTLPGEGKDRIFKVSIKWVSCVSLQALHDALSGRLPSVPFETIQALDVVMRHLPSMRYTPVGRSFFTASEGCSNPLGGGREVWFGFHQSVRPSLWKMMLNIDVSATAFYKAQPVIEFVCEVLDFKSIEEQQKPLTDSQRVKFTKEIKGLKVEITHCGQMKRKYRVCNVTRRPASHQTFPLQQESGQTVECTVAQYFKDRHKLVLRYPHLPCLQVGQEQKHTYLPLEVCNIVAGQRCIKKLTDNQTSTMIRATARSAPDRQEEISKLMRSASFNTDPYVREFGIMVKDEMTDVTGRVLQPPSILYGGRNKAIATPVQGVWDMRNKQFHTGIEIKVWAIACFAPQRQCTEVHLKSFTEQLRKISRDAGMPIQGQPCFCKYAQGADSVEPMFRHLKNTYAGLQLVVVILPGKTPVYAEVKRVGDTVLGMATQCVQMKNVQRTTPQTLSNLCLKINVKLGGVNNILLPQGRPPVFQQPVIFLGADVTHPPAGDGKKPSIAAVVGSMDAHPNRYCATVRVQQHRQEIIQDLAAMVRELLIQFYKSTRFKPTRIIFYRDGVSEGQFQQVLHHELLAIREACIKLEKDYQPGITFIVVQKRHHTRLFCTDKNERVGKSGNIPAGTTVDTKITHPTEFDFYLCSHAGIQGTSRPSHYHVLWDDNRFSSDELQILTYQLCHTYVRCTRSVSIPAPAYYAHLVAFRARYHLVDKEHDSAEGSHTSGQSNGRDHQALAKAVQVHQDTLRTMYFA

Source

organism	Homo sapiens
common_name	human
ncbi_taxonomy_id	9606
host_organism	Trichoplusia ni

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6. Citations (1)

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length_a	1.000 Å
length_b	1.000 Å
length_c	1.000 Å
angle_alpha	90.00 °
angle_beta	90.00 °
angle_gamma	90.00 °
z_pdb	—

space_group	P 1
int_tables_number	1
crystal_system	—

resolution	3.020 Å
resolution_low	— Å
r_factor	—
r_work	—
r_free	—

resolution	3.02 Å
resolution_method	—
num_particles	200460

fit	/app/data/saxs_computation/0b/10be/10be.fit
pddf	/app/data/pr_computation/0b/10be/10be.pr.out
profile	/app/data/saxs_computation/0b/10be/10be.dat

Human AGO2 bound to a miR-20a guide and a position 10-11 mismatched target

SAXS Profile

P(r) Distribution

1. Structure Basics

Download Data

2. SAXS Parameters (CRYSOL)

3. P(r) Analysis (GNOM)

4. Crystallography & Experiment

Unit Cell

Symmetry

Refinement

EM 3D

5. Entities & Polymer Info (3)

Entity 1 polymer

Polymer

Entity 2 polymer

Polymer

Entity 3 polymer

Polymer

Source

6. Citations (1)

7. Files & Curves (10)

Files (7)

Curves (3)