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9w5i

AGO maturation complex (AMC): AGO2-miRNA duplex in complex with Hsp90 beta and co-chaperone p23

Method: ELECTRON MICROSCOPY
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1. Protein Identity and Related Structures Protein Identity & Related Structures

Heat shock protein HSP 90-beta

Homo sapiens

UniProt P08238

State in the Current Structure

Assembly Physical composition Protein state Molecular copy count Associated components Data consistency
1 Protein–RNA Heteromer Protein 4 RNA 2 Prostaglandin E synthase 3 × 1 （Q15185） Protein argonaute-2 × 1 （Q9UKV8） ;RNA (5'-R(P*UP*GP*AP*GP*GP*UP*AP*GP*UP*AP*GP*GP*UP*UP*GP*UP*AP*UP*AP*GP*UP*U)-3') ; × 1 ;RNA (5'-R(P*CP*UP*AP*UP*AP*CP*AP*AP*UP*CP*UP*AP*CP*UP*GP*UP*CP*UP*UP*UP*CP*U)-3') ; × 1 ADENOSINE-5'-TRIPHOSPHATE × 2 MAGNESIUM ION × 3 Consistent with all polymers

Other States of the Same Protein in the Database

Monomer
1uym 3nmq 3pry 3pry 3pry 5uc4 5uc4 5uc4 5uc4 5uch 5uch 5uch

32 assemblies; showing the first 12

Homodimer
8eob

1 assemblies

Homotetramer

None

Other homooligomers

None

Heteromeric or nucleic-acid complex
1qz2 3fwv 3fwv 5fwk 5fwl 5fwm 5fwp 7z37 7z38 7zr0 7zr5 7zr6

18 assemblies; showing the first 12

View Construct and Data Evidence
UniProt name HS90B_HUMAN
Isoform —
PDB entities 1
Chains and sequence ranges Author chain A； PDBConstruct 1–724； UniProt 1–724 Author chain B； PDBConstruct 1–724； UniProt 1–724

Prostaglandin E synthase 3

Homo sapiens

UniProt Q15185

State in the Current Structure

Assembly Physical composition Protein state Molecular copy count Associated components Data consistency
1 Protein–RNA Heteromer Protein 4 RNA 2 Heat shock protein HSP 90-beta × 2 （P08238） Protein argonaute-2 × 1 （Q9UKV8） ;RNA (5'-R(P*UP*GP*AP*GP*GP*UP*AP*GP*UP*AP*GP*GP*UP*UP*GP*UP*AP*UP*AP*GP*UP*U)-3') ; × 1 ;RNA (5'-R(P*CP*UP*AP*UP*AP*CP*AP*AP*UP*CP*UP*AP*CP*UP*GP*UP*CP*UP*UP*UP*CP*U)-3') ; × 1 ADENOSINE-5'-TRIPHOSPHATE × 2 MAGNESIUM ION × 3 Consistent with all polymers

Other States of the Same Protein in the Database

Monomer

None

Homodimer
1ejf

1 assemblies

Homotetramer

None

Other homooligomers

None

Heteromeric or nucleic-acid complex
7krj 7l7i 7l7j 9w5i

4 assemblies

View Construct and Data Evidence
UniProt name TEBP_HUMAN
Isoform —
PDB entities 2
Chains and sequence ranges Author chain C； PDBConstruct 1–160； UniProt 1–160

Protein argonaute-2

Homo sapiens

UniProt Q9UKV8

State in the Current Structure

Assembly Physical composition Protein state Molecular copy count Associated components Data consistency
1 Protein–RNA Heteromer Protein 4 RNA 2 Heat shock protein HSP 90-beta × 2 （P08238） Prostaglandin E synthase 3 × 1 （Q15185） ;RNA (5'-R(P*UP*GP*AP*GP*GP*UP*AP*GP*UP*AP*GP*GP*UP*UP*GP*UP*AP*UP*AP*GP*UP*U)-3') ; × 1 ;RNA (5'-R(P*CP*UP*AP*UP*AP*CP*AP*AP*UP*CP*UP*AP*CP*UP*GP*UP*CP*UP*UP*UP*CP*U)-3') ; × 1 ADENOSINE-5'-TRIPHOSPHATE × 2 MAGNESIUM ION × 3 Consistent with all polymers

Other States of the Same Protein in the Database

Monomer
3luc 3luc 3luc 3lud 3lud 3lud 3lug 3lug 3lug 3luh 3luh 3luh

39 assemblies; showing the first 12

Homodimer

None

Homotetramer

None

Other homooligomers
9bez 9bf0 9bf2

3 assemblies

Heteromeric or nucleic-acid complex
10be 11gi 11gj 11gk 4f3t 4ola 4olb 4w5n 4w5o 4w5q 4w5r 4w5t

55 assemblies; showing the first 12

View Construct and Data Evidence
UniProt name AGO2_HUMAN
Isoform —
PDB entities 3
Chains and sequence ranges Author chain D； PDBConstruct 1–859； UniProt 1–859

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

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2. Structure Basics 2. Structure Basics

Entry ID entry_id9w5i
Deposition date deposition_date2025-08-01
Structure title titleAGO maturation complex (AMC): AGO2-miRNA duplex in complex with Hsp90 beta and co-chaperone p23
Keywords keywordsHsp90, p23, Argonaute, miRNA, AGO2, chaperone, co-chaperone; CHAPERONE
Experimental Method methodELECTRON MICROSCOPY
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3. Official assembly/model SAXS Official SAXS Profiles

This page reads only the latest assembly calculations. Every curve maps to an explicit PDB entry, official biological assembly and coordinate model.

9w5i__assembly_1__model_1

Assembly 1 · Model 1 · CRYSOL 4.1.3-1-20251215 (887e7ef)

Download this curve (.dat)

9w5i__assembly_1__model_1 | I(q)

10-2 10-1 106 107 108 109 q (1/Angstrom) I(q)
100 plotted points; both axes use logarithmic scales.

9w5i__assembly_1__model_1 | P(r) · Pending

The new assembly/model P(r) has not been calculated yet This placeholder does not display legacy data r (Angstrom) P(r)
P(r) will be calculated and displayed separately for the same assembly/model.
Rg（Guinier）46.87 Å
Rg (electron density)45.72 Å
Total Rg46.11 Å
Atom count18185
Residues2172
Excluded volume320850 ų
Maximum q0.500 Å⁻¹
Assembly Model Structure unit Oligomeric description Status CRYSOL Actions
1 1 9w5i__assembly_1__model_1 hexameric （6） Success 4.1.3-1-20251215 (887e7ef) View Download
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4. Crystallography and Experiment 4. Crystallography & Experiment

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5. Entities and Polymers Entities & Polymers (7)

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7. Citations (1)