← Back to search

1a02

STRUCTURE OF THE DNA BINDING DOMAINS OF NFAT, FOS AND JUN BOUND TO DNA

Method: X-RAY DIFFRACTION D_max: 93.4 Å Quality: GOOD

SAXS Profile

P(r) Distribution

▼

1. Structure Basics

entry_id	1a02
deposition_date	1997-12-08
title	STRUCTURE OF THE DNA BINDING DOMAINS OF NFAT, FOS AND JUN BOUND TO DNA
keywords	;TRANSCRIPTION FACTOR, NFAT, NF-AT, AP-1, FOS-JUN, QUATERNARY PROTEIN-DNA COMPLEX, TRANSCRIPTION SYNERGY, COMBINATORIAL GENE REGULATION, TRANSCRIPTION-DNA COMPLEX ;; TRANSCRIPTION/DNA
method	X-RAY DIFFRACTION

▼

Download Data

SAXS curve (.dat) P(r) distribution (.pr.out) Structure (CIF)

▼

2. SAXS Parameters (CRYSOL)

rg_guinier	26.88 Å
rg_electron	25.98 Å
i0	69842100.00
molecular_weight	56004.0 kDa
excluded_volume	66281 Å³
envelope_volume	88672 Å³
shell_volume	29290 Å³
envelope_diameter	96.8 Å
shell_rg	32.44 Å
envelope_rg	26.00 Å
shape_rg	25.92 Å
total_rg	26.76 Å
total_atoms	3886
n_residues	425
n_harmonics	20
q_range	— – 0.5000 Å⁻¹
n_points	101
shell_type	directional
solvent_density	0.3340 e/Å³
contrast_shell	0.0300 e/Å³
crysol_version	4.1.3

▼

3. P(r) Analysis (GNOM)

dmax	93.4 Å
rg_real	26.86 Å
rg_real_error	0.73 Å
i0_real	6.9840e+07
i0_real_error	1.0230e+06
rg_reciprocal	26.86 Å
i0_reciprocal	69840000.0000
total_estimate	0.8709
solution_quality	GOOD a GOOD solution
n_peaks	2
r_peak_primary	34.8 Å
skewness	0.323
kurtosis	-0.227
angular_range	— – 0.2950 Å⁻¹
current_alpha	0.0000
highest_alpha	10030000.0000
n_real_points	60
gnom_version	4.1.3
quality_criteria	AN1: 0.000; Oscil: 0.796; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.940; Smooth: 0.990

▶

4. Crystallography & Experiment

▶

5. Entities & Polymer Info (6)

Entity 1 polymer

description	;DNA (5'-D(DTPDTPDGPDGPDAPDAPDAPDAPDTPDTPDTPDGPDTPDTPDTPDCPDAPDTPDAPDG)-3') ;
formula_weight	6178.0 kDa
src_method	syn
entity_count	1

Polymer

type	polydeoxyribonucleotide
strand_id	A
atom_count	3974

TTGGAAAATTTGTTTCATAG

Entity 2 polymer

description	;DNA (5'-D(DAPDAPDCPDTPDAPDTPDGPDAPDAPDAPDCPDAPDAPDAPDTPDTPDTPDTPDCPDC)-3') ;
formula_weight	6085.0 kDa
src_method	syn
entity_count	1

Polymer

type	polydeoxyribonucleotide
strand_id	B

AACTATGAAACAAATTTTCC

Entity 3 polymer

description	NUCLEAR FACTOR OF ACTIVATED T CELLS
formula_weight	34270.9 kDa
src_method	man
entity_count	1

Polymer

type	polypeptide(L)
strand_id	N

MRGSHHHHHHTDPHASSVPLEWPLSSQSGSYELRIEVQPKPHHRAHYETEGSRGAVKAPTGGHPVVQLHGYMENKPLGLQIFIGTADERILKPHAFYQVHRITGKTVTTTSYEKIVGNTKVLEIPLEPKNNMRATIDCAGILKLRNADIELRKGETDIGRKNTRVRLVFRVHIPESSGRIVSLQTASNPIECSQRSAHELPMVERQDTDSCLVYGGQQMILTGQNFTSESKVVFTEKTTDGQQIWEMEATVDKDKSQPNMLFVEIPEYRNKHIRTPVKVNFYVINGKRKRSQPQHFTYHPV

Source

organism	Homo sapiens
common_name	human
ncbi_taxonomy_id	9606
host_organism	Escherichia coli BL21(DE3)

Entity 4 polymer

description	AP-1 FRAGMENT FOS
formula_weight	6727.7 kDa
mutation	C154S
src_method	man
entity_count	1

Polymer

type	polypeptide(L)
strand_id	F

MKRRIRRERNKMAAAKSRNRRRELTDTLQAETDQLEDEKSALQTEIANLLKEKEKL

Source

organism	Homo sapiens
common_name	human
ncbi_taxonomy_id	9606
host_organism	Escherichia coli

Entity 5 polymer

description	AP-1 FRAGMENT JUN
formula_weight	6583.8 kDa
mutation	C279S
src_method	man
entity_count	1

Polymer

type	polypeptide(L)
strand_id	J

MKAERKRMRNRIAASKSRKRKLERIARLEEKVKTLKAQNSELASTANMLREQVAQL

Source

organism	Homo sapiens
common_name	human
ncbi_taxonomy_id	9606
host_organism	Escherichia coli

Entity 6 water

description	water
formula_weight	18.0 kDa
src_method	nat
entity_count	88

▶

6. Citations (1)

▶

length_a	64.660 Å
length_b	85.460 Å
length_c	83.370 Å
angle_alpha	90.00 °
angle_beta	112.03 °
angle_gamma	90.00 °
z_pdb	2

space_group	P 1 21 1
int_tables_number	4
crystal_system	—

resolution	2.700 Å
resolution_low	10.000 Å
r_factor	0.2460
r_work	0.2460
r_free	0.3030

fit	/app/data/saxs_computation/a0/1a02/1a02.fit
pddf	/app/data/pr_computation/a0/1a02/1a02.pr.out
profile	/app/data/saxs_computation/a0/1a02/1a02.dat

STRUCTURE OF THE DNA BINDING DOMAINS OF NFAT, FOS AND JUN BOUND TO DNA

SAXS Profile

P(r) Distribution

1. Structure Basics

Download Data

2. SAXS Parameters (CRYSOL)

3. P(r) Analysis (GNOM)

4. Crystallography & Experiment

Unit Cell

Symmetry

Refinement

Diffraction

5. Entities & Polymer Info (6)

Entity 1 polymer

Polymer

Entity 2 polymer

Polymer

Entity 3 polymer

Polymer

Source

Entity 4 polymer

Polymer

Source

Entity 5 polymer

Polymer

Source

Entity 6 water

6. Citations (1)

7. Files & Curves (10)

Files (7)

Curves (3)