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1eah

PV2L COMPLEXED WITH ANTIVIRAL AGENT SCH48973

Method: X-RAY DIFFRACTION Dmax: 93.5 Å Quality: REASONABLE
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1. Protein Identity and Related Structures Protein Identity & Related Structures

POLIOVIRUS TYPE 2 COAT PROTEINS VP1 TO VP4

Human poliovirus 2

UniProt P06210

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein homooligomer Homooligomer Protein × 240 PDB declaration: 240-MERIC（240） Consistent with protein copy count Chain 1； UniProt 578–878 Chain 2； UniProt 69–339 Chain 3； UniProt 340–577 Chain 4； UniProt 1–68 Not recorded SC4 1[2-CHLORO-4-METHOXY-PHENYL-OXYMETHYL]-4-[2,6-DICHLORO-PHENYL-OXYMETHYL]-BENZENE × 60 MYR MYRISTIC ACID × 60 X-RAY DIFFRACTION mmCIF provides none of the parsed experimental conditions Resolution 2.90 Å
2 Protein homooligomer Homooligomer Protein × 4 PDB declaration: tetrameric（4） Consistent with protein copy count Chain 1； UniProt 578–878 Chain 2； UniProt 69–339 Chain 3； UniProt 340–577 Chain 4； UniProt 1–68 Not recorded SC4 1[2-CHLORO-4-METHOXY-PHENYL-OXYMETHYL]-4-[2,6-DICHLORO-PHENYL-OXYMETHYL]-BENZENE × 1 MYR MYRISTIC ACID × 1 X-RAY DIFFRACTION mmCIF provides none of the parsed experimental conditions Resolution 2.90 Å
3 Protein homooligomer Homooligomer Protein × 20 PDB declaration: eicosameric（20） Consistent with protein copy count Chain 1； UniProt 578–878 Chain 2； UniProt 69–339 Chain 3； UniProt 340–577 Chain 4； UniProt 1–68 Not recorded SC4 1[2-CHLORO-4-METHOXY-PHENYL-OXYMETHYL]-4-[2,6-DICHLORO-PHENYL-OXYMETHYL]-BENZENE × 5 MYR MYRISTIC ACID × 5 X-RAY DIFFRACTION mmCIF provides none of the parsed experimental conditions Resolution 2.90 Å
4 Protein homooligomer Homooligomer Protein × 24 PDB declaration: 24-meric（24） Consistent with protein copy count Chain 1； UniProt 578–878 Chain 2； UniProt 69–339 Chain 3； UniProt 340–577 Chain 4； UniProt 1–68 Not recorded SC4 1[2-CHLORO-4-METHOXY-PHENYL-OXYMETHYL]-4-[2,6-DICHLORO-PHENYL-OXYMETHYL]-BENZENE × 6 MYR MYRISTIC ACID × 6 X-RAY DIFFRACTION mmCIF provides none of the parsed experimental conditions Resolution 2.90 Å
5 Protein homooligomer Homooligomer Protein × 4 PDB declaration: tetrameric（4） Consistent with protein copy count Chain 1； UniProt 578–878 Chain 2； UniProt 69–339 Chain 3； UniProt 340–577 Chain 4； UniProt 1–68 Not recorded SC4 1[2-CHLORO-4-METHOXY-PHENYL-OXYMETHYL]-4-[2,6-DICHLORO-PHENYL-OXYMETHYL]-BENZENE × 1 MYR MYRISTIC ACID × 1 X-RAY DIFFRACTION mmCIF provides none of the parsed experimental conditions Resolution 2.90 Å
6 Protein homooligomer Homooligomer Protein × 120 PDB declaration: 120-meric（120） Consistent with protein copy count Chain 1； UniProt 578–878 Chain 2； UniProt 69–339 Chain 3； UniProt 340–577 Chain 4； UniProt 1–68 Not recorded SC4 1[2-CHLORO-4-METHOXY-PHENYL-OXYMETHYL]-4-[2,6-DICHLORO-PHENYL-OXYMETHYL]-BENZENE × 30 MYR MYRISTIC ACID × 30 X-RAY DIFFRACTION mmCIF provides none of the parsed experimental conditions Resolution 2.90 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 1EAH Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
3EPF CryoEM structure of poliovirus receptor bound to poliovirus type 2 Deposited 2008-09-29 Different construct Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 300 PDB declaration: 300-MERIC
Chain 1 602–879（278 aa）
Chain 2 79–340（262 aa）
Chain 3 341–575（235 aa）
Chain 4 2–69（68 aa）
Not recorded SC4 1[2-CHLORO-4-METHOXY-PHENYL-OXYMETHYL]-4-[2,6-DICHLORO-PHENYL-OXYMETHYL]-BENZENE × 60 MYR MYRISTIC ACID × 60 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；10mM Tris-HCl, 20mM NaCl
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 9.00 Å
3EPF CryoEM structure of poliovirus receptor bound to poliovirus type 2 Deposited 2008-09-29 Different construct Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 5 PDB declaration: pentameric
Chain 1 602–879（278 aa）
Chain 2 79–340（262 aa）
Chain 3 341–575（235 aa）
Chain 4 2–69（68 aa）
Not recorded SC4 1[2-CHLORO-4-METHOXY-PHENYL-OXYMETHYL]-4-[2,6-DICHLORO-PHENYL-OXYMETHYL]-BENZENE × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；10mM Tris-HCl, 20mM NaCl
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 9.00 Å
3EPF CryoEM structure of poliovirus receptor bound to poliovirus type 2 Deposited 2008-09-29 Different construct Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 3 Protein heterocomplex Heteromer；Protein × 25 PDB declaration: 25-meric
Chain 1 602–879（278 aa）
Chain 2 79–340（262 aa）
Chain 3 341–575（235 aa）
Chain 4 2–69（68 aa）
Not recorded SC4 1[2-CHLORO-4-METHOXY-PHENYL-OXYMETHYL]-4-[2,6-DICHLORO-PHENYL-OXYMETHYL]-BENZENE × 5 MYR MYRISTIC ACID × 5 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；10mM Tris-HCl, 20mM NaCl
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 9.00 Å
3EPF CryoEM structure of poliovirus receptor bound to poliovirus type 2 Deposited 2008-09-29 Different construct Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 4 Protein heterocomplex Heteromer；Protein × 30 PDB declaration: 30-meric
Chain 1 602–879（278 aa）
Chain 2 79–340（262 aa）
Chain 3 341–575（235 aa）
Chain 4 2–69（68 aa）
Not recorded SC4 1[2-CHLORO-4-METHOXY-PHENYL-OXYMETHYL]-4-[2,6-DICHLORO-PHENYL-OXYMETHYL]-BENZENE × 6 MYR MYRISTIC ACID × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；10mM Tris-HCl, 20mM NaCl
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 9.00 Å
3EPF CryoEM structure of poliovirus receptor bound to poliovirus type 2 Deposited 2008-09-29 Different construct Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 5 Protein heterocomplex Heteromer；Protein × 5 PDB declaration: pentameric
Chain 1 602–879（278 aa）
Chain 2 79–340（262 aa）
Chain 3 341–575（235 aa）
Chain 4 2–69（68 aa）
Not recorded SC4 1[2-CHLORO-4-METHOXY-PHENYL-OXYMETHYL]-4-[2,6-DICHLORO-PHENYL-OXYMETHYL]-BENZENE × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；10mM Tris-HCl, 20mM NaCl
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 9.00 Å
8AYZ Poliovirus type 2 (strain MEF-1) virus-like particle in complex with capsid binder compound 17 Deposited 2022-09-04 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 180 PDB declaration: 180-meric
Chain A 579–879（301 aa）
Chain B 1–340（340 aa）
Chain C 342–578（237 aa）
Not recorded SPH SPHINGOSINE × 60 FHK 4-[[4-[1,3-bis(oxidanylidene)isoindol-2-yl]phenyl]sulfonylamino]benzoic acid × 60 ELECTRON MICROSCOPY
cryo-EM buffer pH 7；1 x DPBS was made from tissue culture grade Dulbecco's Phosphate Buffered Saline (Sigma-Aldrich). 20 mM EDTA was prepared from fresh stocks of buffered EDTA.
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；Single blotting with 3.5ul of sample. Four second blot time and -17 blot force on the vitrobot.
Resolution 1.88 Å
9F59 Poliovirus type 2 (strain MEF-1) stabilised virus-like particle (PV2 SC6b) from a mammalian expression system. Deposited 2024-04-28 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 180 PDB declaration: 180-meric
Chain A 579–879（301 aa）
Chain B 2–340（339 aa）
Chain C 341–578（238 aa）
Mutation：VP1 V107I, VP1 F134L, VP1 V183L Mutation：VP2 I57V, VP2 D126A Mutation：VP3 Q178L SPH SPHINGOSINE × 60 ELECTRON MICROSCOPY
cryo-EM buffer pH 7；1 x DPBS, 20 mM EDTA, pH 7.0
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 ul of sample blotted for 3.5 seconds with -15 blot force on FEI Vitrobot mark IV.
Resolution 2.30 Å
9F5P Poliovirus type 2 (strain MEF-1) stabilised virus-like particle (PV2 SC6b) from an insect cell expression system. Deposited 2024-04-29 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 180 PDB declaration: 180-meric
Chain A 579–879（301 aa）
Chain B 2–340（339 aa）
Chain C 341–578（238 aa）
Mutation：VP1 V107I, VP1 V134L, VP1 V183L Mutation：VP2 I57V, VP2 D126A Mutation：VP3 Q178L SPH SPHINGOSINE × 60 ELECTRON MICROSCOPY
cryo-EM buffer pH 7；1 x DPBS, 20 mM EDTA, pH 7.0
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 ul of sample blotted for 3.5 seconds with -15 blot force on FEI Vitrobot mark IV.
Resolution 2.60 Å
9H93 Poliovirus type 2 (strain MEF-1) stabilised virus-like particle (PV2 SC6b) from a yeast expression system. Deposited 2024-10-29 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 180 PDB declaration: 180-meric
Chain B 1–340（340 aa）
Mutation：VP4 I57V, VP2 D57A SPH SPHINGOSINE × 60 ELECTRON MICROSCOPY
cryo-EM buffer pH 7；1 x DPBS, 20 mM EDTA, pH 7.0
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；3ul of sample double blotted for 3.5 seconds with -15 blot force on FEI Vitrobot mark IV.
Resolution 2.40 Å
9H94 Poliovirus type 2 (strain MEF-1) stabilised virus-like particle (PV2 SC5a) from a yeast expression system. Deposited 2024-10-29 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 180 PDB declaration: 180-meric
Chain B 1–340（340 aa）
Not recorded SPH SPHINGOSINE × 60 ELECTRON MICROSCOPY
cryo-EM buffer pH 7；1 x DPBS, 20 mM EDTA, pH 7.0
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；3ul of sample double blotted for 3.5 seconds with -15 blot force on FEI Vitrobot mark IV.
Resolution 2.10 Å
9OCL PV2-10D2 Complex Deposited 2025-04-24 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 360 PDB declaration: 360-meric
Chain 1 579–879（301 aa）
Chain 2 70–340（271 aa）
Not recorded PLM PALMITIC ACID × 60 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.65 Å
9OCL PV2-10D2 Complex Deposited 2025-04-24 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain 1 579–879（301 aa）
Chain 2 70–340（271 aa）
Not recorded PLM PALMITIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.65 Å
9OCL PV2-10D2 Complex Deposited 2025-04-24 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 3 Protein heterocomplex Heteromer；Protein × 30 PDB declaration: 30-meric
Chain 1 579–879（301 aa）
Chain 2 70–340（271 aa）
Not recorded PLM PALMITIC ACID × 5 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.65 Å
9OCL PV2-10D2 Complex Deposited 2025-04-24 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 4 Protein heterocomplex Heteromer；Protein × 36 PDB declaration: 36-meric
Chain 1 579–879（301 aa）
Chain 2 70–340（271 aa）
Not recorded PLM PALMITIC ACID × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.65 Å
9OCL PV2-10D2 Complex Deposited 2025-04-24 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 5 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain 1 579–879（301 aa）
Chain 2 70–340（271 aa）
Not recorded PLM PALMITIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.65 Å

7 other PDB entries and 15 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name POLG_POL2L
Isoform —
PDB entities 1, 2, 3, 4
Chains and sequence ranges Author chain 1； PDBConstruct 1–301； UniProt 578–878 Author chain 2； PDBConstruct 1–271； UniProt 69–339 Author chain 3； PDBConstruct 1–238； UniProt 340–577 Author chain 4； PDBConstruct 1–68； UniProt 1–68

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

SAXS scattering curve SAXS Profile

SAXS profile for 1eah

P(r) Distance Distribution P(r) Distribution

P(r) distribution for 1eah
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2. Structure Basics 2. Structure Basics

Entry ID entry_id1eah
Deposition date deposition_date1997-07-22
Structure title titlePV2L COMPLEXED WITH ANTIVIRAL AGENT SCH48973
Keywords keywordsTYPE 2, LANSING, ANTIVIRAL, PICORNAVIRUS, MOUSE NEUROVIRULENCE, COAT PROTEIN, Icosahedral virus, Virus; VIRUS
Experimental Method methodX-RAY DIFFRACTION
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3. SAXS Parameters (CRYSOL theoretical calculation) 3. SAXS Parameters (CRYSOL)

Radius of gyration Rg (Guinier) rg_guinier29.98 Å
Radius of gyration Rg (electron density) rg_electron28.94 Å
Forward intensity I(0) i0138370000.00
Molecular weight molecular_weight93086.0 kDa
Excluded volume excluded_volume116290 ų
Envelope volume envelope_volume145000 ų
Hydration-shell volume shell_volume40739 ų
Envelope diameter envelope_diameter103.1 Å
Shell Rg shell_rg37.08 Å
Envelope Rg envelope_rg29.56 Å
Shape Rg shape_rg28.93 Å
Total Rg total_rg29.71 Å
Total atoms total_atoms6552
Residues n_residues837
Spherical-harmonic order n_harmonics20
q range q_range— – 0.5000 Å−1
Data points n_points101
Shell type shell_typedirectional
Solvent electron density solvent_density0.3340 e/ų
Shell contrast contrast_shell0.0300 e/ų
CRYSOL version crysol_version4.1.3
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4. P(r) Distance Distribution (GNOM inversion) 4. P(r) Analysis (GNOM)

Maximum dimension Dmax dmax93.5 Å
Rg (real space) rg_real29.90 Å
Rg uncertainty (real space) rg_real_error0.54 Å
I(0) (real space) i0_real1.3840e+08
I(0) uncertainty (real space) i0_real_error1.9710e+06
Rg (reciprocal space) rg_reciprocal29.93 Å
I(0) (reciprocal space) i0_reciprocal138400000.0000
Solution quality estimate total_estimate0.6777
Solution quality rating solution_quality REASONABLE a REASONABLE solution
P(r) peaks n_peaks2
Primary peak position r_peak_primary35.3 Å
Skewness Skewness skewness0.281
Kurtosis Kurtosis kurtosis-0.377
Angular range angular_range— – 0.2650 Å−1
Current regularization parameter α current_alpha0.0000
Highest regularization parameter α highest_alpha23760000.0000
Real-space data points n_real_points54
GNOM version gnom_version4.1.3
Quality Criteria quality_criteria AN1: 0.000; Oscil: 0.928; Stabil: 1.000; Sysdev: 0.050; Positv: 1.000; Valcen: 0.996; Smooth: 0.873
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5. Crystallography and Experiment 5. Crystallography & Experiment

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6. Entities and Polymers Entities & Polymers (7)

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7. Fold Classification (SCOP + CATH) 7 domains

SCOP 2.08 (3 domains)

Domain ID domain_idd1eah.1
Class classb — All beta proteins
Fold Fold foldb.121 — Nucleoplasmin-like/VP (viral coat and capsid proteins)
Superfamily Superfamily superfamilyb.121.4 — Positive stranded ssRNA viruses
Family Family familyb.121.4.1 — Picornaviridae-like VP (VP1, VP2, VP3 and VP4)
Domain ID domain_idd1eah1_
Class classb — All beta proteins
Fold Fold foldb.121 — Nucleoplasmin-like/VP (viral coat and capsid proteins)
Superfamily Superfamily superfamilyb.121.4 — Positive stranded ssRNA viruses
Family Family familyb.121.4.1 — Picornaviridae-like VP (VP1, VP2, VP3 and VP4)
Domain ID domain_idd1eah3_
Class classb — All beta proteins
Fold Fold foldb.121 — Nucleoplasmin-like/VP (viral coat and capsid proteins)
Superfamily Superfamily superfamilyb.121.4 — Positive stranded ssRNA viruses
Family Family familyb.121.4.1 — Picornaviridae-like VP (VP1, VP2, VP3 and VP4)

CATH v4.4 (4 domains)

Domain ID domain_id1eah100
Class class2 — Mainly Beta
Architecture architecture60 — Sandwich
Topology topology120 — Jelly Rolls
Homologous superfamily homologous superfamily20 —
Domain ID domain_id1eah200
Class class2 — Mainly Beta
Architecture architecture60 — Sandwich
Topology topology120 — Jelly Rolls
Homologous superfamily homologous superfamily20 —
Domain ID domain_id1eah300
Class class2 — Mainly Beta
Architecture architecture60 — Sandwich
Topology topology120 — Jelly Rolls
Homologous superfamily homologous superfamily20 —
Domain ID domain_id1eah400
Class class4 — Few Secondary Structures
Architecture architecture10 — Irregular
Topology topology80 — Rhinovirus 14, subunit 4
Homologous superfamily homologous superfamily10 — Picornavirus coat protein VP4
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8. Citations (6)

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9. Files and Curves (10)