2bs0

MS2 (N87AE89K mutant) - Variant Qbeta RNA hairpin complex

Method: X-RAY DIFFRACTION Dmax: 92.0 Å Quality: GOOD

1. Protein Identity and Related Structures Protein Identity & Related Structures

COAT PROTEIN

BACTERIOPHAGE MS2

UniProt P03612

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein–RNA Homooligomer Protein × 180 RNA 120 PDB declaration: 300-MERIC(300) Consistent with all polymer counts Chain A; UniProt 1–129 Chain B; UniProt 1–129 Chain C; UniProt 1–129 Mutation:YES ;5'-R(*AP*UP*GP*CP*AP*UP*GP*UP*CP*UP *AP*AP*GP*AP*CP*UP*GP*CP*AP*U)-3' ; × 120 X-RAY DIFFRACTION mmCIF provides none of the parsed experimental conditions Resolution 2.45 Å R-free 0.246
2 Protein–RNA Homooligomer Protein × 3 RNA 2 PDB declaration: pentameric(5) Consistent with all polymer counts Chain A; UniProt 1–129 Chain B; UniProt 1–129 Chain C; UniProt 1–129 Mutation:YES ;5'-R(*AP*UP*GP*CP*AP*UP*GP*UP*CP*UP *AP*AP*GP*AP*CP*UP*GP*CP*AP*U)-3' ; × 2 X-RAY DIFFRACTION mmCIF provides none of the parsed experimental conditions Resolution 2.45 Å R-free 0.246
3 Protein–RNA Homooligomer Protein × 15 RNA 10 PDB declaration: 25-meric(25) Consistent with all polymer counts Chain A; UniProt 1–129 Chain B; UniProt 1–129 Chain C; UniProt 1–129 Mutation:YES ;5'-R(*AP*UP*GP*CP*AP*UP*GP*UP*CP*UP *AP*AP*GP*AP*CP*UP*GP*CP*AP*U)-3' ; × 10 X-RAY DIFFRACTION mmCIF provides none of the parsed experimental conditions Resolution 2.45 Å R-free 0.246
4 Protein–RNA Homooligomer Protein × 18 RNA 12 PDB declaration: 30-meric(30) Consistent with all polymer counts Chain A; UniProt 1–129 Chain B; UniProt 1–129 Chain C; UniProt 1–129 Mutation:YES ;5'-R(*AP*UP*GP*CP*AP*UP*GP*UP*CP*UP *AP*AP*GP*AP*CP*UP*GP*CP*AP*U)-3' ; × 12 X-RAY DIFFRACTION mmCIF provides none of the parsed experimental conditions Resolution 2.45 Å R-free 0.246
5 Protein–RNA Homooligomer Protein × 3 RNA 2 PDB declaration: pentameric(5) Consistent with all polymer counts Chain A; UniProt 1–129 Chain B; UniProt 1–129 Chain C; UniProt 1–129 Mutation:YES ;5'-R(*AP*UP*GP*CP*AP*UP*GP*UP*CP*UP *AP*AP*GP*AP*CP*UP*GP*CP*AP*U)-3' ; × 2 X-RAY DIFFRACTION mmCIF provides none of the parsed experimental conditions Resolution 2.45 Å R-free 0.246
6 Protein–RNA Homooligomer Protein × 180 RNA 120 PDB declaration: 300-meric(300) Consistent with all polymer counts Chain A; UniProt 1–129 Chain B; UniProt 1–129 Chain C; UniProt 1–129 Mutation:YES ;5'-R(*AP*UP*GP*CP*AP*UP*GP*UP*CP*UP *AP*AP*GP*AP*CP*UP*GP*CP*AP*U)-3' ; × 120 X-RAY DIFFRACTION mmCIF provides none of the parsed experimental conditions Resolution 2.45 Å R-free 0.246

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

43 other PDB entries and 203 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name COAT_BPMS2
Isoform
PDB entities 1
Chains and sequence ranges Author chain A; PDBConstruct 1–129; UniProt 1–129 Author chain B; PDBConstruct 1–129; UniProt 1–129 Author chain C; PDBConstruct 1–129; UniProt 1–129

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

SAXS scattering curve SAXS Profile

SAXS profile for 2bs0

P(r) Distance Distribution P(r) Distribution

P(r) distribution for 2bs0
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2. Structure Basics 2. Structure Basics

Entry ID entry_id2bs0
Deposition date deposition_date2005-05-13
Structure title titleMS2 (N87AE89K mutant) - Variant Qbeta RNA hairpin complex
Keywords keywords;VIRUS/RNA, CAPSID, COMPLEX (CAPSID PROTEIN-RNA HAIRPIN), HAIRPIN, LEVIVIRUS, VIRUS/VIRAL PROTEIN/RNA, RNA-BINDING, STRUCTURAL PROTEIN, VIRUS COAT PROTEIN, ICOSAHEDRAL VIRUS, VIRUS-RNA complex ;; VIRUS/RNA
Experimental Method methodX-RAY DIFFRACTION

3. SAXS Parameters (CRYSOL theoretical calculation) 3. SAXS Parameters (CRYSOL)

Radius of gyration Rg (Guinier) rg_guinier27.07
Radius of gyration Rg (electron density) rg_electron26.56
Forward intensity I(0) i045667000.00
Molecular weight molecular_weight47715.0 kDa
Excluded volume excluded_volume57787 ų
Envelope volume envelope_volume78554 ų
Hydration-shell volume shell_volume25872 ų
Envelope diameter envelope_diameter93.6
Shell Rg shell_rg32.42
Envelope Rg envelope_rg26.93
Shape Rg shape_rg26.58
Total Rg total_rg27.12
Total atoms total_atoms3327
Residues n_residues408
Spherical-harmonic order n_harmonics20
q range q_range— – 0.5000 −1
Data points n_points101
Shell type shell_typedirectional
Solvent electron density solvent_density0.3340 e/ų
Shell contrast contrast_shell0.0300 e/ų
CRYSOL version crysol_version4.1.3

4. P(r) Distance Distribution (GNOM inversion) 4. P(r) Analysis (GNOM)

Maximum dimension Dmax dmax92.0
Rg (real space) rg_real27.18
Rg uncertainty (real space) rg_real_error1.01
I(0) (real space) i0_real4.5670e+07
I(0) uncertainty (real space) i0_real_error7.9400e+05
Rg (reciprocal space) rg_reciprocal27.15
I(0) (reciprocal space) i0_reciprocal45670000.0000
Solution quality estimate total_estimate0.8634
Solution quality rating solution_quality GOOD a GOOD solution
P(r) peaks n_peaks2
Primary peak position r_peak_primary29.6
Skewness Skewness skewness0.454
Kurtosis Kurtosis kurtosis-0.291
Angular range angular_range— – 0.2950 −1
Current regularization parameter α current_alpha0.0000
Highest regularization parameter α highest_alpha3655000.0000
Real-space data points n_real_points60
GNOM version gnom_version4.1.3
Quality Criteria quality_criteria AN1: 0.000; Oscil: 0.777; Stabil: 0.999; Sysdev: 1.000; Positv: 1.000; Valcen: 0.912; Smooth: 0.980

5. Crystallography and Experiment 5. Crystallography & Experiment

6. Entities and Polymers Entities & Polymers (3)

7. Fold Classification (SCOP + CATH) 3 domains

CATH v4.4 (3 domains)

Domain ID domain_id2bs0A00
Class class3 — Alpha Beta
Architecture architecture30 — 2-Layer Sandwich
Topology topology380 — MS2 Viral Coat Protein
Homologous superfamily homologous superfamily10 — MS2 Viral Coat Protein
Domain ID domain_id2bs0B00
Class class3 — Alpha Beta
Architecture architecture30 — 2-Layer Sandwich
Topology topology380 — MS2 Viral Coat Protein
Homologous superfamily homologous superfamily10 — MS2 Viral Coat Protein
Domain ID domain_id2bs0C00
Class class3 — Alpha Beta
Architecture architecture30 — 2-Layer Sandwich
Topology topology380 — MS2 Viral Coat Protein
Homologous superfamily homologous superfamily10 — MS2 Viral Coat Protein

8. Citations (1)

9. Files and Curves (10)