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2vfx

Structure of the Symmetric Mad2 Dimer

Method: X-RAY DIFFRACTION
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1. Protein Identity and Related Structures Protein Identity & Related Structures

MITOTIC SPINDLE ASSEMBLY CHECKPOINT PROTEIN MAD2A

HOMO SAPIENS

UniProt Q13257

State in the Current Structure

Assembly Physical composition Protein state Molecular copy count Associated components Data consistency
1 Protein monomer Monomer Protein 1 MAGNESIUM ION × 1 CHLORIDE ION × 3 2-{2-[2-(2-{2-[2-(2-ETHOXY-ETHOXY)-ETHOXY]-ETHOXY}-ETHOXY)-ETHOXY]-ETHOXY}-ETHANOL × 1 water × 1 Consistent with protein count
10 Protein monomer Monomer Protein 1 CHLORIDE ION × 5 water × 1 Consistent with protein count
11 Protein monomer Monomer Protein 1 MAGNESIUM ION × 1 CHLORIDE ION × 2 3,6,9,12,15,18,21,24,27,30,33,36,39-TRIDECAOXAHENTETRACONTANE-1,41-DIOL × 1 water × 1 Consistent with protein count
12 Protein monomer Monomer Protein 1 MAGNESIUM ION × 1 CHLORIDE ION × 3 2-{2-[2-(2-{2-[2-(2-ETHOXY-ETHOXY)-ETHOXY]-ETHOXY}-ETHOXY)-ETHOXY]-ETHOXY}-ETHANOL × 1 water × 1 Consistent with protein count
2 Protein monomer Monomer Protein 1 MAGNESIUM ION × 1 CHLORIDE ION × 4 3,6,9,12,15,18,21,24,27,30,33,36,39-TRIDECAOXAHENTETRACONTANE-1,41-DIOL × 1 water × 1 Consistent with protein count
3 Protein monomer Monomer Protein 1 MAGNESIUM ION × 1 CHLORIDE ION × 3 3,6,9,12,15,18,21,24,27,30,33,36,39-TRIDECAOXAHENTETRACONTANE-1,41-DIOL × 1 water × 1 Consistent with protein count
4 Protein monomer Monomer Protein 1 CHLORIDE ION × 5 water × 1 Consistent with protein count
5 Protein monomer Monomer Protein 1 MAGNESIUM ION × 1 CHLORIDE ION × 5 2-{2-[2-(2-{2-[2-(2-ETHOXY-ETHOXY)-ETHOXY]-ETHOXY}-ETHOXY)-ETHOXY]-ETHOXY}-ETHANOL × 1 water × 1 Consistent with protein count
6 Protein monomer Monomer Protein 1 MAGNESIUM ION × 1 CHLORIDE ION × 2 3,6,9,12,15,18,21,24,27,30,33,36,39-TRIDECAOXAHENTETRACONTANE-1,41-DIOL × 1 water × 1 Consistent with protein count
7 Protein monomer Monomer Protein 1 MAGNESIUM ION × 1 CHLORIDE ION × 1 3,6,9,12,15,18,21,24,27,30,33,36,39-TRIDECAOXAHENTETRACONTANE-1,41-DIOL × 1 water × 1 Consistent with protein count
8 Protein monomer Monomer Protein 1 3,6,9,12,15,18,21,24,27,30,33,36,39-TRIDECAOXAHENTETRACONTANE-1,41-DIOL × 1 water × 1 Consistent with protein count
9 Protein monomer Monomer Protein 1 CHLORIDE ION × 4 DI(HYDROXYETHYL)ETHER × 1 water × 1 Consistent with protein count

Other States of the Same Protein in the Database

Monomer
1duj 1s2h 2vfx 2vfx 2vfx 2vfx 2vfx 2vfx 2vfx 2vfx 2vfx 2vfx

14 assemblies; showing the first 12

Homodimer
3gmh 3gmh 3gmh 3gmh 3gmh 3gmh

6 assemblies

Homotetramer

None

Other homooligomers

None

Heteromeric or nucleic-acid complex
1go4 1klq 2qyf 2qyf 2v64 2v64 2v64 5khu 5lcw 6f0x 6tlj

11 assemblies

View Construct and Data Evidence
UniProt name MD2L1_HUMAN
Isoform —
PDB entities 1
Chains and sequence ranges Author chain A； PDBConstruct 2–206； UniProt 1–205 Author chain B； PDBConstruct 2–206； UniProt 1–205 Author chain C； PDBConstruct 2–206； UniProt 1–205 Author chain D； PDBConstruct 2–206； UniProt 1–205 Author chain E； PDBConstruct 2–206； UniProt 1–205 Author chain F； PDBConstruct 2–206； UniProt 1–205 Author chain G； PDBConstruct 2–206； UniProt 1–205 Author chain H； PDBConstruct 2–206； UniProt 1–205 Author chain I； PDBConstruct 2–206； UniProt 1–205 Author chain J； PDBConstruct 2–206； UniProt 1–205 Author chain K； PDBConstruct 2–206； UniProt 1–205 Author chain L； PDBConstruct 2–206； UniProt 1–205

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

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2. Structure Basics 2. Structure Basics

Entry ID entry_id2vfx
Deposition date deposition_date2007-11-05
Structure title titleStructure of the Symmetric Mad2 Dimer
Keywords keywordsMAD2, MAD1, CDC2, NUCLEUS, MITOSIS, ANAPHASE, CELL CYCLE, CELL DIVISION, SPINDLE CHECKPOINT, ANAPHASE-PROMOTING COMPLEX; CELL CYCLE
Experimental Method methodX-RAY DIFFRACTION
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3. Official assembly/model SAXS Official SAXS Profiles

This page reads only the latest assembly calculations. Every curve maps to an explicit PDB entry, official biological assembly and coordinate model.

2vfx__assembly_1__model_1

Assembly 1 · Model 1 · CRYSOL 4.1.3-1-20251215 (887e7ef)

Download this curve (.dat)

2vfx__assembly_1__model_1 | I(q)

10-2 10-1 105 106 q (1/Angstrom) I(q)
100 plotted points; both axes use logarithmic scales.

2vfx__assembly_1__model_1 | P(r) · Pending

The new assembly/model P(r) has not been calculated yet This placeholder does not display legacy data r (Angstrom) P(r)
P(r) will be calculated and displayed separately for the same assembly/model.
Rg（Guinier）19.46 Å
Rg (electron density)18.22 Å
Total Rg19.44 Å
Atom count1649
Residues203
Excluded volume29685 ų
Maximum q0.500 Å⁻¹
Assembly Model Structure unit Oligomeric description Status CRYSOL Actions
1 1 2vfx__assembly_1__model_1 monomeric （1） Success 4.1.3-1-20251215 (887e7ef) View Download
2 1 2vfx__assembly_2__model_1 monomeric （1） Success 4.1.3-1-20251215 (887e7ef) View Download
3 1 2vfx__assembly_3__model_1 monomeric （1） Success 4.1.3-1-20251215 (887e7ef) View Download
4 1 2vfx__assembly_4__model_1 monomeric （1） Success 4.1.3-1-20251215 (887e7ef) View Download
5 1 2vfx__assembly_5__model_1 monomeric （1） Success 4.1.3-1-20251215 (887e7ef) View Download
6 1 2vfx__assembly_6__model_1 monomeric （1） Success 4.1.3-1-20251215 (887e7ef) View Download
7 1 2vfx__assembly_7__model_1 monomeric （1） Success 4.1.3-1-20251215 (887e7ef) View Download
8 1 2vfx__assembly_8__model_1 monomeric （1） Success 4.1.3-1-20251215 (887e7ef) View Download
9 1 2vfx__assembly_9__model_1 monomeric （1） Success 4.1.3-1-20251215 (887e7ef) View Download
10 1 2vfx__assembly_10__model_1 monomeric （1） Success 4.1.3-1-20251215 (887e7ef) View Download
11 1 2vfx__assembly_11__model_1 monomeric （1） Success 4.1.3-1-20251215 (887e7ef) View Download
12 1 2vfx__assembly_12__model_1 monomeric （1） Success 4.1.3-1-20251215 (887e7ef) View Download
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4. Crystallography and Experiment 4. Crystallography & Experiment

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5. Entities and Polymers Entities & Polymers (7)

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6. Fold Classification (SCOP + CATH) 24 domains

SCOP 2.08 (12 domains)

Domain ID domain_idd2vfxa_
Class classd — Alpha and beta proteins (a+b)
Fold Fold foldd.135 — The spindle assembly checkpoint protein mad2
Superfamily Superfamily superfamilyd.135.1 — The spindle assembly checkpoint protein mad2
Family Family familyd.135.1.1 — The spindle assembly checkpoint protein mad2
Domain ID domain_idd2vfxb_
Class classd — Alpha and beta proteins (a+b)
Fold Fold foldd.135 — The spindle assembly checkpoint protein mad2
Superfamily Superfamily superfamilyd.135.1 — The spindle assembly checkpoint protein mad2
Family Family familyd.135.1.1 — The spindle assembly checkpoint protein mad2
Domain ID domain_idd2vfxc_
Class classd — Alpha and beta proteins (a+b)
Fold Fold foldd.135 — The spindle assembly checkpoint protein mad2
Superfamily Superfamily superfamilyd.135.1 — The spindle assembly checkpoint protein mad2
Family Family familyd.135.1.1 — The spindle assembly checkpoint protein mad2
Domain ID domain_idd2vfxd_
Class classd — Alpha and beta proteins (a+b)
Fold Fold foldd.135 — The spindle assembly checkpoint protein mad2
Superfamily Superfamily superfamilyd.135.1 — The spindle assembly checkpoint protein mad2
Family Family familyd.135.1.1 — The spindle assembly checkpoint protein mad2
Domain ID domain_idd2vfxe_
Class classd — Alpha and beta proteins (a+b)
Fold Fold foldd.135 — The spindle assembly checkpoint protein mad2
Superfamily Superfamily superfamilyd.135.1 — The spindle assembly checkpoint protein mad2
Family Family familyd.135.1.1 — The spindle assembly checkpoint protein mad2
Domain ID domain_idd2vfxf_
Class classd — Alpha and beta proteins (a+b)
Fold Fold foldd.135 — The spindle assembly checkpoint protein mad2
Superfamily Superfamily superfamilyd.135.1 — The spindle assembly checkpoint protein mad2
Family Family familyd.135.1.1 — The spindle assembly checkpoint protein mad2
Domain ID domain_idd2vfxg_
Class classd — Alpha and beta proteins (a+b)
Fold Fold foldd.135 — The spindle assembly checkpoint protein mad2
Superfamily Superfamily superfamilyd.135.1 — The spindle assembly checkpoint protein mad2
Family Family familyd.135.1.1 — The spindle assembly checkpoint protein mad2
Domain ID domain_idd2vfxh_
Class classd — Alpha and beta proteins (a+b)
Fold Fold foldd.135 — The spindle assembly checkpoint protein mad2
Superfamily Superfamily superfamilyd.135.1 — The spindle assembly checkpoint protein mad2
Family Family familyd.135.1.1 — The spindle assembly checkpoint protein mad2
Domain ID domain_idd2vfxi_
Class classd — Alpha and beta proteins (a+b)
Fold Fold foldd.135 — The spindle assembly checkpoint protein mad2
Superfamily Superfamily superfamilyd.135.1 — The spindle assembly checkpoint protein mad2
Family Family familyd.135.1.1 — The spindle assembly checkpoint protein mad2
Domain ID domain_idd2vfxj_
Class classd — Alpha and beta proteins (a+b)
Fold Fold foldd.135 — The spindle assembly checkpoint protein mad2
Superfamily Superfamily superfamilyd.135.1 — The spindle assembly checkpoint protein mad2
Family Family familyd.135.1.1 — The spindle assembly checkpoint protein mad2
Domain ID domain_idd2vfxk_
Class classd — Alpha and beta proteins (a+b)
Fold Fold foldd.135 — The spindle assembly checkpoint protein mad2
Superfamily Superfamily superfamilyd.135.1 — The spindle assembly checkpoint protein mad2
Family Family familyd.135.1.1 — The spindle assembly checkpoint protein mad2
Domain ID domain_idd2vfxl_
Class classd — Alpha and beta proteins (a+b)
Fold Fold foldd.135 — The spindle assembly checkpoint protein mad2
Superfamily Superfamily superfamilyd.135.1 — The spindle assembly checkpoint protein mad2
Family Family familyd.135.1.1 — The spindle assembly checkpoint protein mad2

CATH v4.4 (12 domains)

Domain ID domain_id2vfxA00
Class class3 — Alpha Beta
Architecture architecture30 — 2-Layer Sandwich
Topology topology900 — Cell Cycle, Spindle Assembly Checkpoint Protein; Chain A
Homologous superfamily homologous superfamily10 — HORMA domain
Domain ID domain_id2vfxB00
Class class3 — Alpha Beta
Architecture architecture30 — 2-Layer Sandwich
Topology topology900 — Cell Cycle, Spindle Assembly Checkpoint Protein; Chain A
Homologous superfamily homologous superfamily10 — HORMA domain
Domain ID domain_id2vfxC00
Class class3 — Alpha Beta
Architecture architecture30 — 2-Layer Sandwich
Topology topology900 — Cell Cycle, Spindle Assembly Checkpoint Protein; Chain A
Homologous superfamily homologous superfamily10 — HORMA domain
Domain ID domain_id2vfxD00
Class class3 — Alpha Beta
Architecture architecture30 — 2-Layer Sandwich
Topology topology900 — Cell Cycle, Spindle Assembly Checkpoint Protein; Chain A
Homologous superfamily homologous superfamily10 — HORMA domain
Domain ID domain_id2vfxE00
Class class3 — Alpha Beta
Architecture architecture30 — 2-Layer Sandwich
Topology topology900 — Cell Cycle, Spindle Assembly Checkpoint Protein; Chain A
Homologous superfamily homologous superfamily10 — HORMA domain
Domain ID domain_id2vfxF00
Class class3 — Alpha Beta
Architecture architecture30 — 2-Layer Sandwich
Topology topology900 — Cell Cycle, Spindle Assembly Checkpoint Protein; Chain A
Homologous superfamily homologous superfamily10 — HORMA domain
Domain ID domain_id2vfxG00
Class class3 — Alpha Beta
Architecture architecture30 — 2-Layer Sandwich
Topology topology900 — Cell Cycle, Spindle Assembly Checkpoint Protein; Chain A
Homologous superfamily homologous superfamily10 — HORMA domain
Domain ID domain_id2vfxH00
Class class3 — Alpha Beta
Architecture architecture30 — 2-Layer Sandwich
Topology topology900 — Cell Cycle, Spindle Assembly Checkpoint Protein; Chain A
Homologous superfamily homologous superfamily10 — HORMA domain
Domain ID domain_id2vfxI00
Class class3 — Alpha Beta
Architecture architecture30 — 2-Layer Sandwich
Topology topology900 — Cell Cycle, Spindle Assembly Checkpoint Protein; Chain A
Homologous superfamily homologous superfamily10 — HORMA domain
Domain ID domain_id2vfxJ00
Class class3 — Alpha Beta
Architecture architecture30 — 2-Layer Sandwich
Topology topology900 — Cell Cycle, Spindle Assembly Checkpoint Protein; Chain A
Homologous superfamily homologous superfamily10 — HORMA domain
Domain ID domain_id2vfxK00
Class class3 — Alpha Beta
Architecture architecture30 — 2-Layer Sandwich
Topology topology900 — Cell Cycle, Spindle Assembly Checkpoint Protein; Chain A
Homologous superfamily homologous superfamily10 — HORMA domain
Domain ID domain_id2vfxL00
Class class3 — Alpha Beta
Architecture architecture30 — 2-Layer Sandwich
Topology topology900 — Cell Cycle, Spindle Assembly Checkpoint Protein; Chain A
Homologous superfamily homologous superfamily10 — HORMA domain
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7. Citations (1)