Ryanodine receptor 1
Oryctolagus cuniculus
State in the Current Structure
| Assembly | Oligomeric State | Construct | Mutations and Modifications | Ligands, Ions and Associated Components | Method and Experimental Conditions | Structure Quality |
|---|---|---|---|---|---|---|
| 1 | Protein monomer Monomer Protein × 1 PDB declaration: monomeric（1） Consistent with protein copy count | Chain A； UniProt 9–205 | Fragment：N-Terminal Domain | No other associated polymer | X-RAY DIFFRACTION X-ray crystallization conditions：VAPOR DIFFUSION, HANGING DROP；pH 7；295 K；15-25% PEG 3350, 0.2M sodium chloride, 0.1M BisTris, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K | Resolution 2.90 Å R-free 0.320 |
| 2 | Protein monomer Monomer Protein × 1 PDB declaration: monomeric（1） Consistent with protein copy count | Chain B； UniProt 9–205 | Fragment：N-Terminal Domain | No other associated polymer | X-RAY DIFFRACTION X-ray crystallization conditions：VAPOR DIFFUSION, HANGING DROP；pH 7；295 K；15-25% PEG 3350, 0.2M sodium chloride, 0.1M BisTris, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K | Resolution 2.90 Å R-free 0.320 |
| 3 | Protein monomer Monomer Protein × 1 PDB declaration: monomeric（1） Consistent with protein copy count | Chain C； UniProt 9–205 | Fragment：N-Terminal Domain | No other associated polymer | X-RAY DIFFRACTION X-ray crystallization conditions：VAPOR DIFFUSION, HANGING DROP；pH 7；295 K；15-25% PEG 3350, 0.2M sodium chloride, 0.1M BisTris, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K | Resolution 2.90 Å R-free 0.320 |
| 4 | Protein monomer Monomer Protein × 1 PDB declaration: monomeric（1） Consistent with protein copy count | Chain D； UniProt 9–205 | Fragment：N-Terminal Domain | No other associated polymer | X-RAY DIFFRACTION X-ray crystallization conditions：VAPOR DIFFUSION, HANGING DROP；pH 7；295 K；15-25% PEG 3350, 0.2M sodium chloride, 0.1M BisTris, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K | Resolution 2.90 Å R-free 0.320 |
| 5 | Protein monomer Monomer Protein × 1 PDB declaration: monomeric（1） Consistent with protein copy count | Chain E； UniProt 9–205 | Fragment：N-Terminal Domain | No other associated polymer | X-RAY DIFFRACTION X-ray crystallization conditions：VAPOR DIFFUSION, HANGING DROP；pH 7；295 K；15-25% PEG 3350, 0.2M sodium chloride, 0.1M BisTris, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K | Resolution 2.90 Å R-free 0.320 |
| 6 | Protein monomer Monomer Protein × 1 PDB declaration: monomeric（1） Consistent with protein copy count | Chain F； UniProt 9–205 | Fragment：N-Terminal Domain | No other associated polymer | X-RAY DIFFRACTION X-ray crystallization conditions：VAPOR DIFFUSION, HANGING DROP；pH 7；295 K；15-25% PEG 3350, 0.2M sodium chloride, 0.1M BisTris, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K | Resolution 2.90 Å R-free 0.320 |
| 7 | Protein monomer Monomer Protein × 1 PDB declaration: monomeric（1） Consistent with protein copy count | Chain G； UniProt 9–205 | Fragment：N-Terminal Domain | No other associated polymer | X-RAY DIFFRACTION X-ray crystallization conditions：VAPOR DIFFUSION, HANGING DROP；pH 7；295 K；15-25% PEG 3350, 0.2M sodium chloride, 0.1M BisTris, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K | Resolution 2.90 Å R-free 0.320 |
| 8 | Protein monomer Monomer Protein × 1 PDB declaration: monomeric（1） Consistent with protein copy count | Chain H； UniProt 9–205 | Fragment：N-Terminal Domain | No other associated polymer | X-RAY DIFFRACTION X-ray crystallization conditions：VAPOR DIFFUSION, HANGING DROP；pH 7；295 K；15-25% PEG 3350, 0.2M sodium chloride, 0.1M BisTris, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K | Resolution 2.90 Å R-free 0.320 |
| 9 | Protein monomer Monomer Protein × 1 PDB declaration: monomeric（1） Consistent with protein copy count | Chain I； UniProt 9–205 | Fragment：N-Terminal Domain | No other associated polymer | X-RAY DIFFRACTION X-ray crystallization conditions：VAPOR DIFFUSION, HANGING DROP；pH 7；295 K；15-25% PEG 3350, 0.2M sodium chloride, 0.1M BisTris, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K | Resolution 2.90 Å R-free 0.320 |
Other States of the Same Protein in the Database
Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.
| Other PDB | Difference from Current Entry 3ILA | Assembly / Oligomeric State | Construct | Mutations and Modifications | Ligands, Ions and Non-polymers | Method and Experimental Conditions | Structure Quality |
|---|---|---|---|---|---|---|---|
| 2BCX Crystal structure of calmodulin in complex with a ryanodine receptor peptide Deposited 2005-10-19 | Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain B
3614–3643（30 aa）
Fragment：residues 3614-3643
|
Not recorded | CA CALCIUM ION × 4 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 4.7；293 K；23% PEG 4000, 0.1 M sodium acetate, 5 mM DTT, pH 4.7, VAPOR DIFFUSION, SITTING DROP, temperature 293K
|
Resolution 2.00 Å R-free 0.249 |
| 2XOA Crystal Structure of the N-terminal three domains of the skeletal muscle Ryanodine Receptor (RyR1) Deposited 2010-08-10 | Different construct Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–559（559 aa）
Fragment：N-TERMINAL DISEASE HOT SPOT, RESIDUES 1-559
|
Not recorded | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 8；pH 8
|
Resolution 2.50 Å R-free 0.234 |
| 3HSM Crystal structure of distal N-terminal beta-trefoil domain of Ryanodine Receptor type 1 Deposited 2009-06-10 | Different construct Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–210（210 aa）
Fragment：sequence database residues 1-210
|
Not recorded | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8；298 K；0.1M MES, 0.1M MgCl2, 24% PEG 3350, 5mM DTT, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 2.50 Å R-free 0.276 |
| 3HSM Crystal structure of distal N-terminal beta-trefoil domain of Ryanodine Receptor type 1 Deposited 2009-06-10 | Different construct Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
1–210（210 aa）
Fragment：sequence database residues 1-210
|
Not recorded | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8；298 K；0.1M MES, 0.1M MgCl2, 24% PEG 3350, 5mM DTT, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 2.50 Å R-free 0.276 |
| 3J8H Structure of the rabbit ryanodine receptor RyR1 in complex with FKBP12 at 3.8 Angstrom resolution Deposited 2014-10-26 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain A
1–1491（1491 aa）
Chain A
1510–1976（467 aa）
Chain A
2057–2460（404 aa）
Chain A
2494–2562（69 aa）
Chain A
2734–2940（207 aa）
Chain A
3645–5037（1393 aa）
Chain C
1–1491（1491 aa）
Chain C
1510–1976（467 aa）
Chain C
2057–2460（404 aa）
Chain C
2494–2562（69 aa）
Chain C
2734–2940（207 aa）
Chain C
3645–5037（1393 aa）
Chain E
1–1491（1491 aa）
Chain E
1510–1976（467 aa）
Chain E
2057–2460（404 aa）
Chain E
2494–2562（69 aa）
Chain E
2734–2940（207 aa）
Chain E
3645–5037（1393 aa）
Chain G
1–1491（1491 aa）
Chain G
1510–1976（467 aa）
Chain G
2057–2460（404 aa）
Chain G
2494–2562（69 aa）
Chain G
2734–2940（207 aa）
Chain G
3645–5037（1393 aa）
|
Not recorded | ZN ZINC ION × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
20 mM MOPS sodium, pH 7.4, 250 mM NaCl, 2 mM DTT, 2 mM EGTA, 0.015% Tween20, protein inhibitors；pH 7.4；20 mM MOPS sodium, pH 7.4, 250 mM NaCl, 2 mM DTT, 2 mM EGTA, 0.015% Tween20, protein inhibitors
cryo-EM vitrification conditions
protein solution was applied to glow-discharged carbon coated grid and blotted for 2 seconds before plunging；Cryogen NITROGEN；Protein solution was applied to glow-discharged carbon coated grid and blotted for 2 seconds before plunging into liquid nitrogen (FEI VITROBOT MARK III)
|
Resolution 3.80 Å |
| 3RQR Crystal structure of the RYR domain of the rabbit ryanodine receptor Deposited 2011-04-28 | Different construct Different oligomeric state Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain A
2733–2940（208 aa）
Fragment：Residues 2733-2940
|
Not recorded | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 8.5；291 K；1.2M Na Citrate, 100mM Tris pH8.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K
|
Resolution 2.16 Å R-free 0.258 |
| 4ERT Crystal structure of rabbit ryanodine receptor 1 (2734-2940) Deposited 2012-04-20 | Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
2734–2940（207 aa）
|
Not recorded | GOL GLYCEROL × 2 K POTASSIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；295 K；19% PEG3350, 30% Glycerol, 0.1M HEPES, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K
|
Resolution 1.95 Å R-free 0.225 |
| 4ESU Crystal structure of rabbit ryanodine receptor 1 mutant S2776M Deposited 2012-04-23 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
2734–2940（207 aa）
|
Mutation：S2776M | GOL GLYCEROL × 5 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；295 K；25% PEG3350, 25% Glycerol, 0.1M HEPES, pH 7.0, vapor diffusion, hanging drop, temperature 295K
|
Resolution 1.59 Å R-free 0.229 |
| 4ETT Crystal structure of rabbit ryanodine receptor 1 mutant E2764K Deposited 2012-04-24 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
2734–2940（207 aa）
|
Mutation：E2764K | GOL GLYCEROL × 4 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；295 K；25% PEG3350, 25% Glycerol, 0.1M HEPES, pH 7.0, vapor diffusion, hanging drop, temperature 295K
|
Resolution 2.19 Å R-free 0.287 |
| 4ETU Crystal structure of rabbit ryanodine receptor 1 mutant R2939S Deposited 2012-04-24 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
2734–2940（207 aa）
|
Mutation：R2939S | GOL GLYCEROL × 3 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；295 K；15% PEG3350, 25% Glycerol, 0.1M HEPES, pH 7.0, vapor diffusion, hanging drop, temperature 295K
|
Resolution 2.19 Å R-free 0.262 |
| 4I0Y CRYSTAL STRUCTURE OF RABBIT RYANODINE RECEPTOR 1 (RESIDUES 1-536) DISEASE MUTANT C36R Deposited 2012-11-19 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–536（536 aa）
Fragment：N-TERMINAL DISEASE HOT SPOT, RESIDUES 1-536
|
Mutation：C36R | GOL GLYCEROL × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
EVAPORATION；pH 9.5；pH 9.5, EVAPORATION
|
Resolution 2.80 Å R-free 0.246 |
| 4I1E Crystal structure of Rabbit Ryanodine Receptor 1 (residues 1-536) disease mutant G249R Deposited 2012-11-20 | Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–536（536 aa）
Fragment：N-TERMINAL DISEASE HOT SPOT, RESIDUES 1-536
|
Mutation：G249R | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
EVAPORATION；pH 8；pH 8.0, EVAPORATION
|
Resolution 2.40 Å R-free 0.258 |
| 4I2S Crystal structure of rabbit ryanodine receptor 1 (residues 1-536) disease mutant I404M Deposited 2012-11-22 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–536（536 aa）
Fragment：N-TERMINAL DISEASE HOT SPOT, RESIDUES 1-536
|
Mutation：I404M | GOL GLYCEROL × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
EVAPORATION；pH 8；pH 8.0, EVAPORATION
|
Resolution 2.50 Å R-free 0.249 |
| 4I37 Crystal structure of rabbit ryanodine receptor 1 (residues 1-536) disease mutant R402G Deposited 2012-11-25 | Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–536（536 aa）
Fragment：N-TERMINAL DISEASE HOT SPOT, RESIDUES 1-536
|
Mutation：R402G | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
EVAPORATION；pH 6.5；pH 6.5, EVAPORATION
|
Resolution 2.95 Å R-free 0.289 |
| 4I3N Crystal structure of rabbit ryanodine receptor 1 (residues 1-536) disease mutant D61N Deposited 2012-11-26 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–536（536 aa）
Fragment：N-TERMINAL DISEASE HOT SPOT, RESIDUES 1-536
|
Mutation：D61N | GOL GLYCEROL × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
EVAPORATION；pH 9.5；pH 9.5, EVAPORATION
|
Resolution 2.95 Å R-free 0.257 |
| 4I6I Crystal structure of rabbit ryanodine receptor 1 (residues 1-559) disease mutant R45C Deposited 2012-11-29 | Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–559（559 aa）
Fragment：N-TERMINAL DISEASE HOT SPOT, RESIDUES 1-559
|
Mutation：R45C | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
EVAPORATION；pH 7；pH 7.0, EVAPORATION
|
Resolution 2.50 Å R-free 0.277 |
| 4I7I Crystal structure of rabbit ryanodine receptor 1 (residues 1-536) disease mutant L14R Deposited 2012-11-30 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–536（536 aa）
Fragment：N-TERMINAL DISEASE HOT SPOT, RESIDUES 1-536
|
Mutation：L14R | GOL GLYCEROL × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
EVAPORATION；pH 8；pH 8.0, EVAPORATION
|
Resolution 2.90 Å R-free 0.249 |
| 4I8M Crystal structure of rabbit Ryanodine receptor 1 (residues 1-536) disease mutant V219I Deposited 2012-12-03 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–536（536 aa）
Fragment：N-TERMINAL DISEASE HOT SPOT, RESIDUES 1-536
|
Mutation：V219I | GOL GLYCEROL × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
EVAPORATION；pH 9；pH 9.0, EVAPORATION
|
Resolution 2.80 Å R-free 0.253 |
| 4I96 Crystal structure of the N-terminal two domains of the skeletal muscle ryanodine receptor (rabbit RyR1) residues 217-536 Deposited 2012-12-04 | Different construct Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
217–536（320 aa）
Fragment：N-TERMINAL DISEASE HOT SPOT, RESIDUES 217-536
|
Not recorded | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
EVAPORATION；pH 8；pH 8.0, EVAPORATION
|
Resolution 2.73 Å R-free 0.241 |
| 4P9J Crystal Structure of rabbit Ryanodine Receptor 1 SPRY2 Domain (1070-1246) Deposited 2014-04-04 | Different construct Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain C
1070–1246（177 aa）
Fragment：UNP Residues 1070-1246
|
Not recorded | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；298.15 K；sodium citrate
|
Resolution 1.84 Å R-free 0.220 |
| 4P9J Crystal Structure of rabbit Ryanodine Receptor 1 SPRY2 Domain (1070-1246) Deposited 2014-04-04 | Different construct Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1070–1246（177 aa）
Fragment：UNP Residues 1070-1246
|
Not recorded | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；298.15 K；sodium citrate
|
Resolution 1.84 Å R-free 0.220 |
| 4P9J Crystal Structure of rabbit Ryanodine Receptor 1 SPRY2 Domain (1070-1246) Deposited 2014-04-04 | Different construct Different experimental conditions Different structure-quality metrics | Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
1070–1246（177 aa）
Fragment：UNP Residues 1070-1246
|
Not recorded | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；298.15 K；sodium citrate
|
Resolution 1.84 Å R-free 0.220 |
| 4UWA Structure of the ryanodine receptor at resolution of 6.1 A in closed state Deposited 2014-08-11 | Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric |
Chain A
1–533（533 aa）
Chain A
649–1571（923 aa）
Chain A
2734–2939（206 aa）
Chain B
1–533（533 aa）
Chain B
649–1571（923 aa）
Chain B
2734–2939（206 aa）
Chain C
1–533（533 aa）
Chain C
649–1571（923 aa）
Chain C
2734–2939（206 aa）
Chain D
1–533（533 aa）
Chain D
649–1571（923 aa）
Chain D
2734–2939（206 aa）
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
10 MM MOPS, 200MM NACL, 2MM DTT, 1MM EGTA；pH 7.4；10 MM MOPS, 200MM NACL, 2MM DTT, 1MM EGTA
cryo-EM vitrification conditions
Cryogen ETHANE；LIQUID ETHANE
|
Resolution 6.10 Å |
| 4UWE Structure of the ryanodine receptor at resolution of 8.5 A in partially open state Deposited 2014-08-11 | Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric |
Chain A
1–533（533 aa）
Chain A
649–1571（923 aa）
Chain A
2734–2939（206 aa）
Chain B
1–533（533 aa）
Chain B
649–1571（923 aa）
Chain B
2734–2939（206 aa）
Chain C
1–533（533 aa）
Chain C
649–1571（923 aa）
Chain C
2734–2939（206 aa）
Chain D
1–533（533 aa）
Chain D
649–1571（923 aa）
Chain D
2734–2939（206 aa）
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
10 MM MOPS, 200 MM NACL, 2 MM DTT AND 10 MM CACL2, 0.2% FLUORINATED OCTYL- MALTOSIDE；pH 7.4；10 MM MOPS, 200 MM NACL, 2 MM DTT AND 10 MM CACL2, 0.2% FLUORINATED OCTYL- MALTOSIDE
cryo-EM vitrification conditions
Cryogen ETHANE；LIQUID ETHANE
|
Resolution 8.50 Å |
| 5C30 Crystal Structure of Rabbit Ryanodine Receptor 1 Repeat12 Domain Deposited 2015-06-16 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
857–1054（198 aa）
Fragment：repeat12 domain (UNP residues 857-1054)
|
Mutation：C906A, C937A, C1040A | IPA ISOPROPYL ALCOHOL × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；277 K；0.1 M HEPES, pH 7.0, 19% w/v PEG20000
|
Resolution 1.55 Å R-free 0.165 |
| 5GKY Structure of RyR1 in a closed state (C1 conformer) Deposited 2016-07-07 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain A
1–5037（5037 aa）
Chain C
1–5037（5037 aa）
Chain E
1–5037（5037 aa）
Chain G
1–5037（5037 aa）
|
Not recorded | ZN ZINC ION × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4；20 mM MOPS-Na, pH 7.4, 250 mM NaCl, 2 mM DTT, 0.015% Tween20 (w/v) (Sigma-Aldrich) and protease inhibitor cocktail including 2 mM PMSF, 2.6 ug/ml aprotinin, 1.4 ug/ml pepstatin, and 10 ug/ml leupeptin (Amresco)
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.80 Å |
| 5GKZ Structure of RyR1 in a closed state (C3 conformer) Deposited 2016-07-07 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain A
1–5037（5037 aa）
Chain C
1–5037（5037 aa）
Chain E
1–5037（5037 aa）
Chain G
1–5037（5037 aa）
|
Not recorded | ZN ZINC ION × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4；20 mM MOPS-Na, pH 7.4, 250 mM NaCl, 2 mM DTT, 0.015% Tween20 (w/v) (Sigma-Aldrich) and protease inhibitor cocktail including 2 mM PMSF, 2.6 ug/ml aprotinin, 1.4 ug/ml pepstatin, and 10 ug/ml leupeptin (Amresco)
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.00 Å |
| 5GL0 Structure of RyR1 in a closed state (C4 conformer) Deposited 2016-07-07 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain A
1–5037（5037 aa）
Chain C
1–5037（5037 aa）
Chain E
1–5037（5037 aa）
Chain G
1–5037（5037 aa）
|
Not recorded | ZN ZINC ION × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4；20 mM MOPS-Na, pH 7.4, 250 mM NaCl, 2 mM DTT, 0.015% Tween20 (w/v) (Sigma-Aldrich) and protease inhibitor cocktail including 2 mM PMSF, 2.6 ug/ml aprotinin, 1.4 ug/ml pepstatin, and 10 ug/ml leupeptin (Amresco)
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.20 Å |
| 5GL1 Structure of RyR1 in an open state Deposited 2016-07-07 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain A
1–5037（5037 aa）
Chain C
1–5037（5037 aa）
Chain E
1–5037（5037 aa）
Chain G
1–5037（5037 aa）
|
Not recorded | ZN ZINC ION × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4；20 mM MOPS-Na, pH 7.4, 250 mM NaCl, 2 mM DTT, 0.5% CHAPS (w/v) (Sigma-Aldrich) and protease inhibitor cocktail including 2 mM PMSF, 2.6 ug/ml aprotinin, 1.4 ug/ml pepstatin, and 10 ug/ml leupeptin (Amresco),50 uM CaCl2 and 10 uM PCB95
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 5.70 Å |
| 5J8V Structure of rabbit ryanodine receptor RyR1 open state activated by calcium ion Deposited 2016-04-08 | Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric |
Chain A
1–5037（5037 aa）
Chain B
1–5037（5037 aa）
Chain C
1–5037（5037 aa）
Chain D
1–5037（5037 aa）
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4；1:1000 diluted protease inhibitor cocktail was also added in buffer
cryo-EM vitrification conditions
Cryogen ETHANE；Grids were blotted for 2s before plunging.
|
Resolution 4.90 Å |
| 5T15 Structural basis for gating and activation of RyR1 (30 uM Ca2+ dataset, all particles) Deposited 2016-08-17 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain B
12–1275（1264 aa）
Chain B
1573–2479（907 aa）
Chain B
2734–2939（206 aa）
Chain B
3639–5037（1399 aa）
Chain E
12–1275（1264 aa）
Chain E
1573–2479（907 aa）
Chain E
2734–2939（206 aa）
Chain E
3639–5037（1399 aa）
Chain G
12–1275（1264 aa）
Chain G
1573–2479（907 aa）
Chain G
2734–2939（206 aa）
Chain G
3639–5037（1399 aa）
Chain I
12–1275（1264 aa）
Chain I
1573–2479（907 aa）
Chain I
2734–2939（206 aa）
Chain I
3639–5037（1399 aa）
|
Not recorded | ZN ZINC ION × 4 CA CALCIUM ION × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE；Blotted for 3-4 seconds on both sides with Whatman ashless filter paper, blot force 3, wait time 30 seconds
|
Resolution 3.60 Å |
| 5T9M Structure of rabbit RyR1 (Ca2+-only dataset, class 1) Deposited 2016-09-09 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain B
12–1275（1264 aa）
Chain B
1573–2479（907 aa）
Chain B
2734–2939（206 aa）
Chain B
3639–5037（1399 aa）
Chain E
12–1275（1264 aa）
Chain E
1573–2479（907 aa）
Chain E
2734–2939（206 aa）
Chain E
3639–5037（1399 aa）
Chain G
12–1275（1264 aa）
Chain G
1573–2479（907 aa）
Chain G
2734–2939（206 aa）
Chain G
3639–5037（1399 aa）
Chain I
12–1275（1264 aa）
Chain I
1573–2479（907 aa）
Chain I
2734–2939（206 aa）
Chain I
3639–5037（1399 aa）
|
Not recorded | ZN ZINC ION × 4 CA CALCIUM ION × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE；Blotted for 3-4 seconds on both sides with Whatman ashless filter paper, blot force 3, wait time 30 seconds
|
Resolution 4.00 Å |
| 5T9N Structure of rabbit RyR1 (Ca2+-only dataset, class 2) Deposited 2016-09-09 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain B
12–1275（1264 aa）
Chain B
1573–2479（907 aa）
Chain B
2734–2939（206 aa）
Chain B
3639–5037（1399 aa）
Chain E
12–1275（1264 aa）
Chain E
1573–2479（907 aa）
Chain E
2734–2939（206 aa）
Chain E
3639–5037（1399 aa）
Chain G
12–1275（1264 aa）
Chain G
1573–2479（907 aa）
Chain G
2734–2939（206 aa）
Chain G
3639–5037（1399 aa）
Chain I
12–1275（1264 aa）
Chain I
1573–2479（907 aa）
Chain I
2734–2939（206 aa）
Chain I
3639–5037（1399 aa）
|
Not recorded | ZN ZINC ION × 4 CA CALCIUM ION × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE；Blotted for 3-4 seconds on both sides with Whatman ashless filter paper, blot force 3, wait time 30 seconds
|
Resolution 3.80 Å |
| 5T9R Structure of rabbit RyR1 (Ca2+-only dataset, class 3) Deposited 2016-09-09 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain B
12–1275（1264 aa）
Chain B
1573–2479（907 aa）
Chain B
2734–2939（206 aa）
Chain B
3639–5037（1399 aa）
Chain E
12–1275（1264 aa）
Chain E
1573–2479（907 aa）
Chain E
2734–2939（206 aa）
Chain E
3639–5037（1399 aa）
Chain G
12–1275（1264 aa）
Chain G
1573–2479（907 aa）
Chain G
2734–2939（206 aa）
Chain G
3639–5037（1399 aa）
Chain I
12–1275（1264 aa）
Chain I
1573–2479（907 aa）
Chain I
2734–2939（206 aa）
Chain I
3639–5037（1399 aa）
|
Not recorded | ZN ZINC ION × 4 CA CALCIUM ION × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE；Blotted for 3-4 seconds on both sides with Whatman ashless filter paper, blot force 3, wait time 30 seconds
|
Resolution 5.80 Å |
| 5T9S Structure of rabbit RyR1 (Ca2+-only dataset, class 4) Deposited 2016-09-09 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain B
12–1275（1264 aa）
Chain B
1573–2479（907 aa）
Chain B
2734–2939（206 aa）
Chain B
3639–5037（1399 aa）
Chain E
12–1275（1264 aa）
Chain E
1573–2479（907 aa）
Chain E
2734–2939（206 aa）
Chain E
3639–5037（1399 aa）
Chain G
12–1275（1264 aa）
Chain G
1573–2479（907 aa）
Chain G
2734–2939（206 aa）
Chain G
3639–5037（1399 aa）
Chain I
12–1275（1264 aa）
Chain I
1573–2479（907 aa）
Chain I
2734–2939（206 aa）
Chain I
3639–5037（1399 aa）
|
Not recorded | ZN ZINC ION × 4 CA CALCIUM ION × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE；Blotted for 3-4 seconds on both sides with Whatman ashless filter paper, blot force 3, wait time 30 seconds
|
Resolution 4.20 Å |
| 5T9V Structure of rabbit RyR1 (Caffeine/ATP/Ca2+ dataset, class 1) Deposited 2016-09-09 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain B
12–1275（1264 aa）
Chain B
1573–2479（907 aa）
Chain B
2734–2939（206 aa）
Chain B
3639–4253（615 aa）
Chain B
4540–5037（498 aa）
Chain E
12–1275（1264 aa）
Chain E
1573–2479（907 aa）
Chain E
2734–2939（206 aa）
Chain E
3639–4253（615 aa）
Chain E
4540–5037（498 aa）
Chain G
12–1275（1264 aa）
Chain G
1573–2479（907 aa）
Chain G
2734–2939（206 aa）
Chain G
3639–4253（615 aa）
Chain G
4540–5037（498 aa）
Chain I
12–1275（1264 aa）
Chain I
1573–2479（907 aa）
Chain I
2734–2939（206 aa）
Chain I
3639–4253（615 aa）
Chain I
4540–5037（498 aa）
|
Not recorded | ATP ADENOSINE-5'-TRIPHOSPHATE × 4 CFF CAFFEINE × 4 ZN ZINC ION × 4 CA CALCIUM ION × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE；Blotted for 3-4 seconds on both sides with Whatman ashless filter paper, blot force 3, wait time 30 seconds
|
Resolution 4.40 Å |
| 5TA3 Structure of rabbit RyR1 (Caffeine/ATP/Ca2+ dataset, class 2) Deposited 2016-09-09 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain B
12–1275（1264 aa）
Chain B
1573–2479（907 aa）
Chain B
2734–2939（206 aa）
Chain B
3639–4253（615 aa）
Chain B
4541–5037（497 aa）
Chain E
12–1275（1264 aa）
Chain E
1573–2479（907 aa）
Chain E
2734–2939（206 aa）
Chain E
3639–4253（615 aa）
Chain E
4541–5037（497 aa）
Chain G
12–1275（1264 aa）
Chain G
1573–2479（907 aa）
Chain G
2734–2939（206 aa）
Chain G
3639–4253（615 aa）
Chain G
4541–5037（497 aa）
Chain I
12–1275（1264 aa）
Chain I
1573–2479（907 aa）
Chain I
2734–2939（206 aa）
Chain I
3639–4253（615 aa）
Chain I
4541–5037（497 aa）
|
Not recorded | ATP ADENOSINE-5'-TRIPHOSPHATE × 4 CFF CAFFEINE × 4 ZN ZINC ION × 4 CA CALCIUM ION × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE；Blotted for 3-4 seconds on both sides with Whatman ashless filter paper, blot force 3, wait time 30 seconds
|
Resolution 4.40 Å |
| 5TAL Structure of rabbit RyR1 (Caffeine/ATP/Ca2+ dataset, class 1&2) Deposited 2016-09-10 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain B
12–1275（1264 aa）
Chain B
1573–2479（907 aa）
Chain B
2734–2939（206 aa）
Chain B
3639–4253（615 aa）
Chain B
4541–5037（497 aa）
Chain E
12–1275（1264 aa）
Chain E
1573–2479（907 aa）
Chain E
2734–2939（206 aa）
Chain E
3639–4253（615 aa）
Chain E
4541–5037（497 aa）
Chain G
12–1275（1264 aa）
Chain G
1573–2479（907 aa）
Chain G
2734–2939（206 aa）
Chain G
3639–4253（615 aa）
Chain G
4541–5037（497 aa）
Chain I
12–1275（1264 aa）
Chain I
1573–2479（907 aa）
Chain I
2734–2939（206 aa）
Chain I
3639–4253（615 aa）
Chain I
4541–5037（497 aa）
|
Not recorded | ATP ADENOSINE-5'-TRIPHOSPHATE × 4 CFF CAFFEINE × 4 ZN ZINC ION × 4 CA CALCIUM ION × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE；Blotted for 3-4 seconds on both sides with Whatman ashless filter paper, blot force 3, wait time 30 seconds
|
Resolution 4.30 Å |
| 5TAM Structure of rabbit RyR1 (Caffeine/ATP/Ca2+ dataset, class 4) Deposited 2016-09-10 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain B
12–1275（1264 aa）
Chain B
1573–2479（907 aa）
Chain B
2734–2939（206 aa）
Chain B
3639–4253（615 aa）
Chain B
4541–5037（497 aa）
Chain E
12–1275（1264 aa）
Chain E
1573–2479（907 aa）
Chain E
2734–2939（206 aa）
Chain E
3639–4253（615 aa）
Chain E
4541–5037（497 aa）
Chain G
12–1275（1264 aa）
Chain G
1573–2479（907 aa）
Chain G
2734–2939（206 aa）
Chain G
3639–4253（615 aa）
Chain G
4541–5037（497 aa）
Chain I
12–1275（1264 aa）
Chain I
1573–2479（907 aa）
Chain I
2734–2939（206 aa）
Chain I
3639–4253（615 aa）
Chain I
4541–5037（497 aa）
|
Not recorded | ATP ADENOSINE-5'-TRIPHOSPHATE × 4 CFF CAFFEINE × 4 ZN ZINC ION × 4 CA CALCIUM ION × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE；Blotted for 3-4 seconds on both sides with Whatman ashless filter paper, blot force 3, wait time 30 seconds
|
Resolution 4.30 Å |
| 5TAN Structure of rabbit RyR1 (Caffeine/ATP/Ca2+ dataset, class 3) Deposited 2016-09-10 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain B
12–1275（1264 aa）
Chain B
1573–2479（907 aa）
Chain B
2734–2939（206 aa）
Chain B
3639–4253（615 aa）
Chain B
4541–5037（497 aa）
Chain E
12–1275（1264 aa）
Chain E
1573–2479（907 aa）
Chain E
2734–2939（206 aa）
Chain E
3639–4253（615 aa）
Chain E
4541–5037（497 aa）
Chain G
12–1275（1264 aa）
Chain G
1573–2479（907 aa）
Chain G
2734–2939（206 aa）
Chain G
3639–4253（615 aa）
Chain G
4541–5037（497 aa）
Chain I
12–1275（1264 aa）
Chain I
1573–2479（907 aa）
Chain I
2734–2939（206 aa）
Chain I
3639–4253（615 aa）
Chain I
4541–5037（497 aa）
|
Not recorded | ATP ADENOSINE-5'-TRIPHOSPHATE × 4 CFF CAFFEINE × 4 ZN ZINC ION × 4 CA CALCIUM ION × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE；Blotted for 3-4 seconds on both sides with Whatman ashless filter paper, blot force 3, wait time 30 seconds
|
Resolution 4.30 Å |
| 5TAP Structure of rabbit RyR1 (Caffeine/ATP/EGTA dataset, all particles) Deposited 2016-09-10 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain B
12–1275（1264 aa）
Chain B
1573–2479（907 aa）
Chain B
2734–2939（206 aa）
Chain B
3639–4253（615 aa）
Chain B
4541–5037（497 aa）
Chain E
12–1275（1264 aa）
Chain E
1573–2479（907 aa）
Chain E
2734–2939（206 aa）
Chain E
3639–4253（615 aa）
Chain E
4541–5037（497 aa）
Chain G
12–1275（1264 aa）
Chain G
1573–2479（907 aa）
Chain G
2734–2939（206 aa）
Chain G
3639–4253（615 aa）
Chain G
4541–5037（497 aa）
Chain I
12–1275（1264 aa）
Chain I
1573–2479（907 aa）
Chain I
2734–2939（206 aa）
Chain I
3639–4253（615 aa）
Chain I
4541–5037（497 aa）
|
Not recorded | ATP ADENOSINE-5'-TRIPHOSPHATE × 4 CFF CAFFEINE × 4 ZN ZINC ION × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE；Blotted for 3-4 seconds on both sides with Whatman ashless filter paper, blot force 3, wait time 30 seconds
|
Resolution 4.40 Å |
| 5TAQ Structure of rabbit RyR1 (Caffeine/ATP/Ca2+ dataset, class 3&4) Deposited 2016-09-10 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain B
12–1275（1264 aa）
Chain B
1573–2479（907 aa）
Chain B
2734–2939（206 aa）
Chain B
3639–4253（615 aa）
Chain B
4541–5037（497 aa）
Chain E
12–1275（1264 aa）
Chain E
1573–2479（907 aa）
Chain E
2734–2939（206 aa）
Chain E
3639–4253（615 aa）
Chain E
4541–5037（497 aa）
Chain G
12–1275（1264 aa）
Chain G
1573–2479（907 aa）
Chain G
2734–2939（206 aa）
Chain G
3639–4253（615 aa）
Chain G
4541–5037（497 aa）
Chain I
12–1275（1264 aa）
Chain I
1573–2479（907 aa）
Chain I
2734–2939（206 aa）
Chain I
3639–4253（615 aa）
Chain I
4541–5037（497 aa）
|
Not recorded | ATP ADENOSINE-5'-TRIPHOSPHATE × 4 CFF CAFFEINE × 4 ZN ZINC ION × 4 CA CALCIUM ION × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE；Blotted for 3-4 seconds on both sides with Whatman ashless filter paper, blot force 3, wait time 30 seconds
|
Resolution 4.10 Å |
| 5TAS Structure of rabbit RyR1 (Caffeine/ATP/EGTA dataset, class 1) Deposited 2016-09-10 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain B
12–1275（1264 aa）
Chain B
1573–2479（907 aa）
Chain B
2734–2939（206 aa）
Chain B
3639–4253（615 aa）
Chain B
4541–5037（497 aa）
Chain E
12–1275（1264 aa）
Chain E
1573–2479（907 aa）
Chain E
2734–2939（206 aa）
Chain E
3639–4253（615 aa）
Chain E
4541–5037（497 aa）
Chain G
12–1275（1264 aa）
Chain G
1573–2479（907 aa）
Chain G
2734–2939（206 aa）
Chain G
3639–4253（615 aa）
Chain G
4541–5037（497 aa）
Chain I
12–1275（1264 aa）
Chain I
1573–2479（907 aa）
Chain I
2734–2939（206 aa）
Chain I
3639–4253（615 aa）
Chain I
4541–5037（497 aa）
|
Not recorded | ATP ADENOSINE-5'-TRIPHOSPHATE × 4 CFF CAFFEINE × 4 ZN ZINC ION × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE；Blotted for 3-4 seconds on both sides with Whatman ashless filter paper, blot force 3, wait time 30 seconds
|
Resolution 6.20 Å |
| 5TAT Structure of rabbit RyR1 (Caffeine/ATP/EGTA dataset, class 2) Deposited 2016-09-10 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain B
12–1275（1264 aa）
Chain B
1573–2479（907 aa）
Chain B
2734–2939（206 aa）
Chain B
3639–4253（615 aa）
Chain B
4541–5037（497 aa）
Chain E
12–1275（1264 aa）
Chain E
1573–2479（907 aa）
Chain E
2734–2939（206 aa）
Chain E
3639–4253（615 aa）
Chain E
4541–5037（497 aa）
Chain G
12–1275（1264 aa）
Chain G
1573–2479（907 aa）
Chain G
2734–2939（206 aa）
Chain G
3639–4253（615 aa）
Chain G
4541–5037（497 aa）
Chain I
12–1275（1264 aa）
Chain I
1573–2479（907 aa）
Chain I
2734–2939（206 aa）
Chain I
3639–4253（615 aa）
Chain I
4541–5037（497 aa）
|
Not recorded | ATP ADENOSINE-5'-TRIPHOSPHATE × 4 CFF CAFFEINE × 4 ZN ZINC ION × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE；Blotted for 3-4 seconds on both sides with Whatman ashless filter paper, blot force 3, wait time 30 seconds
|
Resolution 4.80 Å |
| 5TAU Structure of rabbit RyR1 (Caffeine/ATP/EGTA dataset, class 3) Deposited 2016-09-10 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain B
12–1275（1264 aa）
Chain B
1573–2479（907 aa）
Chain B
2734–2939（206 aa）
Chain B
3639–4253（615 aa）
Chain B
4541–5037（497 aa）
Chain E
12–1275（1264 aa）
Chain E
1573–2479（907 aa）
Chain E
2734–2939（206 aa）
Chain E
3639–4253（615 aa）
Chain E
4541–5037（497 aa）
Chain G
12–1275（1264 aa）
Chain G
1573–2479（907 aa）
Chain G
2734–2939（206 aa）
Chain G
3639–4253（615 aa）
Chain G
4541–5037（497 aa）
Chain I
12–1275（1264 aa）
Chain I
1573–2479（907 aa）
Chain I
2734–2939（206 aa）
Chain I
3639–4253（615 aa）
Chain I
4541–5037（497 aa）
|
Not recorded | ATP ADENOSINE-5'-TRIPHOSPHATE × 4 CFF CAFFEINE × 4 ZN ZINC ION × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE；Blotted for 3-4 seconds on both sides with Whatman ashless filter paper, blot force 3, wait time 30 seconds
|
Resolution 6.20 Å |
| 5TAV Structure of rabbit RyR1 (Caffeine/ATP/EGTA dataset, class 4) Deposited 2016-09-10 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain B
12–1275（1264 aa）
Chain B
1573–2479（907 aa）
Chain B
2734–2939（206 aa）
Chain B
3639–4253（615 aa）
Chain B
4541–5037（497 aa）
Chain E
12–1275（1264 aa）
Chain E
1573–2479（907 aa）
Chain E
2734–2939（206 aa）
Chain E
3639–4253（615 aa）
Chain E
4541–5037（497 aa）
Chain G
12–1275（1264 aa）
Chain G
1573–2479（907 aa）
Chain G
2734–2939（206 aa）
Chain G
3639–4253（615 aa）
Chain G
4541–5037（497 aa）
Chain I
12–1275（1264 aa）
Chain I
1573–2479（907 aa）
Chain I
2734–2939（206 aa）
Chain I
3639–4253（615 aa）
Chain I
4541–5037（497 aa）
|
Not recorded | ATP ADENOSINE-5'-TRIPHOSPHATE × 4 CFF CAFFEINE × 4 ZN ZINC ION × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE；Blotted for 3-4 seconds on both sides with Whatman ashless filter paper, blot force 3, wait time 30 seconds
|
Resolution 4.80 Å |
| 5TAW Structure of rabbit RyR1 (ryanodine dataset, all particles) Deposited 2016-09-10 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain B
12–1275（1264 aa）
Chain B
1573–2479（907 aa）
Chain B
2734–2939（206 aa）
Chain B
3639–4253（615 aa）
Chain B
4541–5037（497 aa）
Chain E
12–1275（1264 aa）
Chain E
1573–2479（907 aa）
Chain E
2734–2939（206 aa）
Chain E
3639–4253（615 aa）
Chain E
4541–5037（497 aa）
Chain G
12–1275（1264 aa）
Chain G
1573–2479（907 aa）
Chain G
2734–2939（206 aa）
Chain G
3639–4253（615 aa）
Chain G
4541–5037（497 aa）
Chain I
12–1275（1264 aa）
Chain I
1573–2479（907 aa）
Chain I
2734–2939（206 aa）
Chain I
3639–4253（615 aa）
Chain I
4541–5037（497 aa）
|
Not recorded | ZN ZINC ION × 4 CA CALCIUM ION × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE；Blotted for 3-4 seconds on both sides with Whatman ashless filter paper, blot force 3, wait time 30 seconds
|
Resolution 4.40 Å |
| 5TAX Structure of rabbit RyR1 (ryanodine dataset, class 1) Deposited 2016-09-10 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain B
12–1275（1264 aa）
Chain B
1573–2479（907 aa）
Chain B
2734–2939（206 aa）
Chain B
3639–4253（615 aa）
Chain B
4541–5037（497 aa）
Chain E
12–1275（1264 aa）
Chain E
1573–2479（907 aa）
Chain E
2734–2939（206 aa）
Chain E
3639–4253（615 aa）
Chain E
4541–5037（497 aa）
Chain G
12–1275（1264 aa）
Chain G
1573–2479（907 aa）
Chain G
2734–2939（206 aa）
Chain G
3639–4253（615 aa）
Chain G
4541–5037（497 aa）
Chain I
12–1275（1264 aa）
Chain I
1573–2479（907 aa）
Chain I
2734–2939（206 aa）
Chain I
3639–4253（615 aa）
Chain I
4541–5037（497 aa）
|
Not recorded | ZN ZINC ION × 4 CA CALCIUM ION × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE；Blotted for 3-4 seconds on both sides with Whatman ashless filter paper, blot force 3, wait time 30 seconds
|
Resolution 6.60 Å |
| 5TAY Structure of rabbit RyR1 (ryanodine dataset, class 2) Deposited 2016-09-10 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain B
12–1275（1264 aa）
Chain B
1573–2479（907 aa）
Chain B
2734–2939（206 aa）
Chain B
3639–4253（615 aa）
Chain B
4541–5037（497 aa）
Chain E
12–1275（1264 aa）
Chain E
1573–2479（907 aa）
Chain E
2734–2939（206 aa）
Chain E
3639–4253（615 aa）
Chain E
4541–5037（497 aa）
Chain G
12–1275（1264 aa）
Chain G
1573–2479（907 aa）
Chain G
2734–2939（206 aa）
Chain G
3639–4253（615 aa）
Chain G
4541–5037（497 aa）
Chain I
12–1275（1264 aa）
Chain I
1573–2479（907 aa）
Chain I
2734–2939（206 aa）
Chain I
3639–4253（615 aa）
Chain I
4541–5037（497 aa）
|
Not recorded | ZN ZINC ION × 4 CA CALCIUM ION × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE；Blotted for 3-4 seconds on both sides with Whatman ashless filter paper, blot force 3, wait time 30 seconds
|
Resolution 4.60 Å |
| 5TAZ Structure of rabbit RyR1 (ryanodine dataset, class 3) Deposited 2016-09-10 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain B
12–1275（1264 aa）
Chain B
1573–2479（907 aa）
Chain B
2734–2939（206 aa）
Chain B
3639–4253（615 aa）
Chain B
4541–5037（497 aa）
Chain E
12–1275（1264 aa）
Chain E
1573–2479（907 aa）
Chain E
2734–2939（206 aa）
Chain E
3639–4253（615 aa）
Chain E
4541–5037（497 aa）
Chain G
12–1275（1264 aa）
Chain G
1573–2479（907 aa）
Chain G
2734–2939（206 aa）
Chain G
3639–4253（615 aa）
Chain G
4541–5037（497 aa）
Chain I
12–1275（1264 aa）
Chain I
1573–2479（907 aa）
Chain I
2734–2939（206 aa）
Chain I
3639–4253（615 aa）
Chain I
4541–5037（497 aa）
|
Not recorded | ZN ZINC ION × 4 CA CALCIUM ION × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE；Blotted for 3-4 seconds on both sides with Whatman ashless filter paper, blot force 3, wait time 30 seconds
|
Resolution 4.30 Å |
| 5TB0 Structure of rabbit RyR1 (EGTA-only dataset, all particles) Deposited 2016-09-10 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain B
12–1275（1264 aa）
Chain B
1573–2479（907 aa）
Chain B
2734–2939（206 aa）
Chain B
3639–4253（615 aa）
Chain B
4541–5037（497 aa）
Chain E
12–1275（1264 aa）
Chain E
1573–2479（907 aa）
Chain E
2734–2939（206 aa）
Chain E
3639–4253（615 aa）
Chain E
4541–5037（497 aa）
Chain G
12–1275（1264 aa）
Chain G
1573–2479（907 aa）
Chain G
2734–2939（206 aa）
Chain G
3639–4253（615 aa）
Chain G
4541–5037（497 aa）
Chain I
12–1275（1264 aa）
Chain I
1573–2479（907 aa）
Chain I
2734–2939（206 aa）
Chain I
3639–4253（615 aa）
Chain I
4541–5037（497 aa）
|
Not recorded | ZN ZINC ION × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE；Blotted for 3-4 seconds on both sides with Whatman ashless filter paper, blot force 3, wait time 30 seconds
|
Resolution 4.40 Å |
| 5TB1 Structure of rabbit RyR1 (EGTA-only dataset, class 1) Deposited 2016-09-10 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain B
12–1275（1264 aa）
Chain B
1573–2479（907 aa）
Chain B
2734–2939（206 aa）
Chain B
3639–4253（615 aa）
Chain B
4541–5037（497 aa）
Chain E
12–1275（1264 aa）
Chain E
1573–2479（907 aa）
Chain E
2734–2939（206 aa）
Chain E
3639–4253（615 aa）
Chain E
4541–5037（497 aa）
Chain G
12–1275（1264 aa）
Chain G
1573–2479（907 aa）
Chain G
2734–2939（206 aa）
Chain G
3639–4253（615 aa）
Chain G
4541–5037（497 aa）
Chain I
12–1275（1264 aa）
Chain I
1573–2479（907 aa）
Chain I
2734–2939（206 aa）
Chain I
3639–4253（615 aa）
Chain I
4541–5037（497 aa）
|
Not recorded | ZN ZINC ION × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE；Blotted for 3-4 seconds on both sides with Whatman ashless filter paper, blot force 3, wait time 30 seconds
|
Resolution 4.60 Å |
| 5TB2 Structure of rabbit RyR1 (EGTA-only dataset, class 2) Deposited 2016-09-11 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain B
12–1275（1264 aa）
Chain B
1573–2479（907 aa）
Chain B
2734–2939（206 aa）
Chain B
3639–4253（615 aa）
Chain B
4541–5037（497 aa）
Chain E
12–1275（1264 aa）
Chain E
1573–2479（907 aa）
Chain E
2734–2939（206 aa）
Chain E
3639–4253（615 aa）
Chain E
4541–5037（497 aa）
Chain G
12–1275（1264 aa）
Chain G
1573–2479（907 aa）
Chain G
2734–2939（206 aa）
Chain G
3639–4253（615 aa）
Chain G
4541–5037（497 aa）
Chain I
12–1275（1264 aa）
Chain I
1573–2479（907 aa）
Chain I
2734–2939（206 aa）
Chain I
3639–4253（615 aa）
Chain I
4541–5037（497 aa）
|
Not recorded | ZN ZINC ION × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE；Blotted for 3-4 seconds on both sides with Whatman ashless filter paper, blot force 3, wait time 30 seconds
|
Resolution 4.60 Å |
| 5TB3 Structure of rabbit RyR1 (EGTA-only dataset, class 3) Deposited 2016-09-11 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain B
12–1275（1264 aa）
Chain B
1573–2479（907 aa）
Chain B
2734–2939（206 aa）
Chain B
3639–4253（615 aa）
Chain B
4541–5037（497 aa）
Chain E
12–1275（1264 aa）
Chain E
1573–2479（907 aa）
Chain E
2734–2939（206 aa）
Chain E
3639–4253（615 aa）
Chain E
4541–5037（497 aa）
Chain G
12–1275（1264 aa）
Chain G
1573–2479（907 aa）
Chain G
2734–2939（206 aa）
Chain G
3639–4253（615 aa）
Chain G
4541–5037（497 aa）
Chain I
12–1275（1264 aa）
Chain I
1573–2479（907 aa）
Chain I
2734–2939（206 aa）
Chain I
3639–4253（615 aa）
Chain I
4541–5037（497 aa）
|
Not recorded | ZN ZINC ION × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE；Blotted for 3-4 seconds on both sides with Whatman ashless filter paper, blot force 3, wait time 30 seconds
|
Resolution 4.70 Å |
| 5TB4 Structure of rabbit RyR1 (EGTA-only dataset, class 4) Deposited 2016-09-11 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain B
12–1275（1264 aa）
Chain B
1573–2479（907 aa）
Chain B
2734–2939（206 aa）
Chain B
3639–4253（615 aa）
Chain B
4541–5037（497 aa）
Chain E
12–1275（1264 aa）
Chain E
1573–2479（907 aa）
Chain E
2734–2939（206 aa）
Chain E
3639–4253（615 aa）
Chain E
4541–5037（497 aa）
Chain G
12–1275（1264 aa）
Chain G
1573–2479（907 aa）
Chain G
2734–2939（206 aa）
Chain G
3639–4253（615 aa）
Chain G
4541–5037（497 aa）
Chain I
12–1275（1264 aa）
Chain I
1573–2479（907 aa）
Chain I
2734–2939（206 aa）
Chain I
3639–4253（615 aa）
Chain I
4541–5037（497 aa）
|
Not recorded | ZN ZINC ION × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE；Blotted for 3-4 seconds on both sides with Whatman ashless filter paper, blot force 3, wait time 30 seconds
|
Resolution 4.50 Å |
| 6FG3 Structure of Ryanodine receptor 1 in nanodiscs in the presence of calcium, ATP and ryanodine Deposited 2018-01-09 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric |
Chain A
1–1275（1275 aa）
Chain A
1573–2479（907 aa）
Chain A
2734–2939（206 aa）
Chain A
3639–5037（1399 aa）
Chain B
1–1275（1275 aa）
Chain B
1573–2479（907 aa）
Chain B
2734–2939（206 aa）
Chain B
3639–5037（1399 aa）
Chain C
1–1275（1275 aa）
Chain C
1573–2479（907 aa）
Chain C
2734–2939（206 aa）
Chain C
3639–5037（1399 aa）
Chain D
1–1275（1275 aa）
Chain D
1573–2479（907 aa）
Chain D
2734–2939（206 aa）
Chain D
3639–5037（1399 aa）
|
Not recorded | ZN ZINC ION × 4 CA CALCIUM ION × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 7.30 Å |
| 6FOO Structure of Ryanodine Receptor 1 in nanodiscs in the presence of calcium and ATP Deposited 2018-02-08 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric |
Chain A
1–1297（1297 aa）
Chain A
1573–2479（907 aa）
Chain A
2734–2939（206 aa）
Chain A
3639–5037（1399 aa）
Chain B
1–1297（1297 aa）
Chain B
1573–2479（907 aa）
Chain B
2734–2939（206 aa）
Chain B
3639–5037（1399 aa）
Chain C
1–1297（1297 aa）
Chain C
1573–2479（907 aa）
Chain C
2734–2939（206 aa）
Chain C
3639–5037（1399 aa）
Chain D
1–1297（1297 aa）
Chain D
1573–2479（907 aa）
Chain D
2734–2939（206 aa）
Chain D
3639–5037（1399 aa）
|
Not recorded | ZN ZINC ION × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 8.20 Å |
| 6M2W Structure of RyR1 (Ca2+/Caffeine/ATP/CaM1234/CHL) Deposited 2020-03-01 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric |
Chain A
1–2634（2634 aa）
Chain A
2741–2939（199 aa）
Chain A
3623–4253（631 aa）
Chain A
4541–5037（497 aa）
Chain D
1–2634（2634 aa）
Chain D
2741–2939（199 aa）
Chain D
3623–4253（631 aa）
Chain D
4541–5037（497 aa）
Chain G
1–2634（2634 aa）
Chain G
2741–2939（199 aa）
Chain G
3623–4253（631 aa）
Chain G
4541–5037（497 aa）
Chain J
1–2634（2634 aa）
Chain J
2741–2939（199 aa）
Chain J
3623–4253（631 aa）
Chain J
4541–5037（497 aa）
|
Not recorded | CFF CAFFEINE × 4 CA CALCIUM ION × 4 ATP ADENOSINE-5'-TRIPHOSPHATE × 4 ZN ZINC ION × 4 F0U 5-bromanyl-N-[4-chloranyl-2-methyl-6-(methylcarbamoyl)phenyl]-2-(3-chloranylpyridin-2-yl)pyrazole-3-carboxamide × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.80 Å |
| 6WOT Cryo-EM structure of recombinant rabbit Ryanodine Receptor type 1 mutant R164C in complex with FKBP12.6 Deposited 2020-04-25 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain A
1–5037（5037 aa）
Chain B
1–5037（5037 aa）
Chain C
1–5037（5037 aa）
Chain D
1–5037（5037 aa）
|
Mutation：R164C Mutation：R164C Mutation：R164C Mutation：R164C | ZN ZINC ION × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.54 Å |
| 7CF9 Structure of RyR1 (Ca2+/CHL) Deposited 2020-06-24 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain A
1–2634（2634 aa）
Chain A
2741–2939（199 aa）
Chain A
3623–4253（631 aa）
Chain A
4541–5037（497 aa）
Chain C
1–2634（2634 aa）
Chain C
2741–2939（199 aa）
Chain C
3623–4253（631 aa）
Chain C
4541–5037（497 aa）
Chain E
1–2634（2634 aa）
Chain E
2741–2939（199 aa）
Chain E
3623–4253（631 aa）
Chain E
4541–5037（497 aa）
Chain G
1–2634（2634 aa）
Chain G
2741–2939（199 aa）
Chain G
3623–4253（631 aa）
Chain G
4541–5037（497 aa）
|
Not recorded | CA CALCIUM ION × 4 ZN ZINC ION × 4 F0U 5-bromanyl-N-[4-chloranyl-2-methyl-6-(methylcarbamoyl)phenyl]-2-(3-chloranylpyridin-2-yl)pyrazole-3-carboxamide × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.70 Å |
| 7M6A High resolution structure of the membrane embedded skeletal muscle ryanodine receptor Deposited 2021-03-25 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain A
1–5037（5037 aa）
Chain B
1–5037（5037 aa）
Chain G
1–5037（5037 aa）
Chain I
1–5037（5037 aa）
|
Not recorded | ATP ADENOSINE-5'-TRIPHOSPHATE × 4 CA CALCIUM ION × 4 ZN ZINC ION × 4 CFF CAFFEINE × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.36 Å |
| 7M6L High resolution structure of the membrane embedded skeletal muscle ryanodine receptor Deposited 2021-03-25 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain A
1–5037（5037 aa）
Chain B
1–5037（5037 aa）
Chain G
1–5037（5037 aa）
Chain I
1–5037（5037 aa）
|
Not recorded | ATP ADENOSINE-5'-TRIPHOSPHATE × 4 CA CALCIUM ION × 4 ZN ZINC ION × 4 CFF CAFFEINE × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.98 Å |
| 7T64 Rabbit RyR1 disease mutant Y523S in complex with FKBP12.6 embedded in lipidic nanodisc in the closed state Deposited 2021-12-13 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain A
1–5037（5037 aa）
Chain B
1–5037（5037 aa）
Chain C
1–5037（5037 aa）
Chain D
1–5037（5037 aa）
|
Mutation：Y523S Mutation：Y523S Mutation：Y523S Mutation：Y523S | ZN ZINC ION × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE；Blot force 2; blot for 1.0s before plunging
|
Resolution 4.00 Å |
| 7T65 Rabbit RyR1 disease mutant Y523S in complex with FKBP12.6 embedded in lipidic nanodisc in the open state Deposited 2021-12-13 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain A
1–5037（5037 aa）
Chain B
1–5037（5037 aa）
Chain C
1–5037（5037 aa）
Chain D
1–5037（5037 aa）
|
Mutation：Y523S Mutation：Y523S Mutation：Y523S Mutation：Y523S | ATP ADENOSINE-5'-TRIPHOSPHATE × 4 CA CALCIUM ION × 4 ZN ZINC ION × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE；Blot force 2; blot for 1.0s before plunging
|
Resolution 4.05 Å |
| 7TDG Rabbit RyR1 with AMP-PCP and high Ca2+ embedded in nanodisc in inactivated conformation (Dataset-A) Deposited 2021-12-31 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric |
Chain A
1–1275（1275 aa）
Chain A
1565–2486（922 aa）
Chain A
2704–2941（238 aa）
Chain A
3614–5037（1424 aa）
Chain B
1–1275（1275 aa）
Chain B
1565–2486（922 aa）
Chain B
2704–2941（238 aa）
Chain B
3614–5037（1424 aa）
Chain C
1–1275（1275 aa）
Chain C
1565–2486（922 aa）
Chain C
2704–2941（238 aa）
Chain C
3614–5037（1424 aa）
Chain D
1–1275（1275 aa）
Chain D
1565–2486（922 aa）
Chain D
2704–2941（238 aa）
Chain D
3614–5037（1424 aa）
|
Not recorded | ZN ZINC ION × 4 CA CALCIUM ION × 8 ACP PHOSPHOMETHYLPHOSPHONIC ACID ADENYLATE ESTER × 4 POV (2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate × 8 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE；Sample was blotted for 1 second on both sides with Whatman hardened ashless filter paper with blot force 2.
|
Resolution 3.80 Å |
| 7TDH Rabbit RyR1 with AMP-PCP and high Ca2+ embedded in nanodisc in open conformation Deposited 2021-12-31 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric |
Chain A
1–1275（1275 aa）
Chain A
1565–2486（922 aa）
Chain A
2704–2941（238 aa）
Chain A
3614–5037（1424 aa）
Chain B
1–1275（1275 aa）
Chain B
1565–2486（922 aa）
Chain B
2704–2941（238 aa）
Chain B
3614–5037（1424 aa）
Chain C
1–1275（1275 aa）
Chain C
1565–2486（922 aa）
Chain C
2704–2941（238 aa）
Chain C
3614–5037（1424 aa）
Chain D
1–1275（1275 aa）
Chain D
1565–2486（922 aa）
Chain D
2704–2941（238 aa）
Chain D
3614–5037（1424 aa）
|
Not recorded | ZN ZINC ION × 4 CA CALCIUM ION × 8 ACP PHOSPHOMETHYLPHOSPHONIC ACID ADENYLATE ESTER × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE；Sample was blotted for 1 second on both sides with Whatman hardened ashless filter paper with blot force 2.
|
Resolution 4.00 Å |
| 7TDI Rabbit RyR1 with AMP-PCP and high Ca2+ embedded in nanodisc in closed-inactivated conformation class 2 (Dataset-A) Deposited 2021-12-31 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric |
Chain A
1–1275（1275 aa）
Chain A
1565–2486（922 aa）
Chain A
2704–2941（238 aa）
Chain A
3614–5037（1424 aa）
Chain B
1–1275（1275 aa）
Chain B
1565–2486（922 aa）
Chain B
2704–2941（238 aa）
Chain B
3614–5037（1424 aa）
Chain C
1–1275（1275 aa）
Chain C
1565–2486（922 aa）
Chain C
2704–2941（238 aa）
Chain C
3614–5037（1424 aa）
Chain D
1–1275（1275 aa）
Chain D
1565–2486（922 aa）
Chain D
2704–2941（238 aa）
Chain D
3614–5037（1424 aa）
|
Not recorded | ACP PHOSPHOMETHYLPHOSPHONIC ACID ADENYLATE ESTER × 4 ZN ZINC ION × 4 CA CALCIUM ION × 8 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE；Sample was blotted for 1 second on both sides with Whatman hardened ashless filter paper with blot force 2.
|
Resolution 3.30 Å |
| 7TDJ Rabbit RyR1 with AMP-PCP and high Ca2+ embedded in nanodisc in closed-inactivated conformation class 1(Dataset-A) Deposited 2021-12-31 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric |
Chain A
1–1275（1275 aa）
Chain A
1565–2486（922 aa）
Chain A
2704–2941（238 aa）
Chain A
3614–5037（1424 aa）
Chain B
1–1275（1275 aa）
Chain B
1565–2486（922 aa）
Chain B
2704–2941（238 aa）
Chain B
3614–5037（1424 aa）
Chain C
1–1275（1275 aa）
Chain C
1565–2486（922 aa）
Chain C
2704–2941（238 aa）
Chain C
3614–5037（1424 aa）
Chain D
1–1275（1275 aa）
Chain D
1565–2486（922 aa）
Chain D
2704–2941（238 aa）
Chain D
3614–5037（1424 aa）
|
Not recorded | ACP PHOSPHOMETHYLPHOSPHONIC ACID ADENYLATE ESTER × 4 ZN ZINC ION × 4 CA CALCIUM ION × 8 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE；Sample was blotted for 1 second on both sides with Whatman hardened ashless filter paper with blot force 2.
|
Resolution 3.70 Å |
| 7TDK Rabbit RyR1 with AMP-PCP and high Ca2+ embedded in nanodisc in closed-inactivated conformation class 3 (Dataset-A) Deposited 2021-12-31 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric |
Chain A
1–1275（1275 aa）
Chain A
1565–2486（922 aa）
Chain A
2704–2941（238 aa）
Chain A
3614–5037（1424 aa）
Chain B
1–1275（1275 aa）
Chain B
1565–2486（922 aa）
Chain B
2704–2941（238 aa）
Chain B
3614–5037（1424 aa）
Chain C
1–1275（1275 aa）
Chain C
1565–2486（922 aa）
Chain C
2704–2941（238 aa）
Chain C
3614–5037（1424 aa）
Chain D
1–1275（1275 aa）
Chain D
1565–2486（922 aa）
Chain D
2704–2941（238 aa）
Chain D
3614–5037（1424 aa）
|
Not recorded | ACP PHOSPHOMETHYLPHOSPHONIC ACID ADENYLATE ESTER × 4 ZN ZINC ION × 4 CA CALCIUM ION × 8 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE；Sample was blotted for 1 second on both sides with Whatman hardened ashless filter paper with blot force 2.
|
Resolution 3.80 Å |
| 7TZC A drug and ATP binding site in type 1 ryanodine receptor Deposited 2022-02-15 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric |
Chain A
1–5037（5037 aa）
Chain B
1–5037（5037 aa）
Chain G
1–5037（5037 aa）
Chain I
1–5037（5037 aa）
|
Not recorded | CA CALCIUM ION × 20 ATP ADENOSINE-5'-TRIPHOSPHATE × 8 ZN ZINC ION × 4 CFF CAFFEINE × 4 KVR 4-[(7-methoxy-2,3-dihydro-1,4-benzothiazepin-4(5H)-yl)methyl]benzoic acid × 4 L9R (2S)-3-(octadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate × 8 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.45 Å |
| 8DRP Focus/local refined map in C4 of signal subtracted RyR1 particles Deposited 2022-07-21 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric |
Chain A
1–5037（5037 aa）
Chain B
1–5037（5037 aa）
Chain C
1–5037（5037 aa）
Chain D
1–5037（5037 aa）
|
Not recorded | CFF CAFFEINE × 4 ATP ADENOSINE-5'-TRIPHOSPHATE × 4 ZN ZINC ION × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.84 Å |
| 8DTB Focus/local refined map in C1 of signal subtracted RyR1 particles in complex with ImperaCalcin Deposited 2022-07-25 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 5 PDB declaration: pentameric |
Chain A
1–5037（5037 aa）
Chain D
1–5037（5037 aa）
Chain G
1–5037（5037 aa）
Chain J
1–5037（5037 aa）
|
Not recorded | ATP ADENOSINE-5'-TRIPHOSPHATE × 4 CFF CAFFEINE × 3 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.14 Å |
| 8DUJ Global map in C1 of RyR1 particles in complex with ImperaCalcin Deposited 2022-07-27 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 13 PDB declaration: tridecameric |
Chain A
1–5037（5037 aa）
Chain D
1–5037（5037 aa）
Chain G
1–5037（5037 aa）
Chain J
1–5037（5037 aa）
|
Not recorded | CFF CAFFEINE × 4 CA CALCIUM ION × 4 ATP ADENOSINE-5'-TRIPHOSPHATE × 4 ZN ZINC ION × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.70 Å |
| 8DVE RyR1 in presence of IpCa-T26E phosphomimetic and activating ligands Deposited 2022-07-28 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric |
Chain A
1–5037（5037 aa）
Chain D
1–5037（5037 aa）
Chain G
1–5037（5037 aa）
Chain J
1–5037（5037 aa）
|
Not recorded | CFF CAFFEINE × 4 CA CALCIUM ION × 4 ATP ADENOSINE-5'-TRIPHOSPHATE × 4 ZN ZINC ION × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.84 Å |
| 8RRT Structure of rabbit RyR1 reconstituted into lipid liposomes in open state in complex with FKBP and Nb9657 Deposited 2024-01-23 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: 12-meric |
Chain B
11–5037（5027 aa）
Chain E
11–5037（5027 aa）
Chain G
11–5037（5027 aa）
Chain J
11–5037（5027 aa）
|
Not recorded | ZN ZINC ION × 4 ATP ADENOSINE-5'-TRIPHOSPHATE × 4 CFF CAFFEINE × 4 CA CALCIUM ION × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.60 Å |
| 8RRU Structure of RyR1 reconstituted into lipid liposomes in primed state in complex with FKBP and Nb9657. Deposited 2024-01-23 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: 12-meric |
Chain B
11–5037（5027 aa）
Chain E
11–5037（5027 aa）
Chain G
11–5037（5027 aa）
Chain J
11–5037（5027 aa）
|
Not recorded | ZN ZINC ION × 4 ATP ADENOSINE-5'-TRIPHOSPHATE × 4 CFF CAFFEINE × 4 CA CALCIUM ION × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.70 Å |
| 8RRV Structure of RyR1 in detergent in close state in complex with FKBP and Nb9657. Deposited 2024-01-23 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: 12-meric |
Chain B
1–5037（5037 aa）
Chain E
1–5037（5037 aa）
Chain G
1–5037（5037 aa）
Chain J
1–5037（5037 aa）
|
Not recorded | ZN ZINC ION × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.20 Å |
| 8RRW Structure of RyR1 in detergent in open state in complex with FKBP and Nb9657. Deposited 2024-01-23 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: 12-meric |
Chain B
11–5037（5027 aa）
Chain E
11–5037（5027 aa）
Chain G
11–5037（5027 aa）
Chain J
11–5037（5027 aa）
|
Not recorded | ZN ZINC ION × 4 ATP ADENOSINE-5'-TRIPHOSPHATE × 4 CFF CAFFEINE × 4 CA CALCIUM ION × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.20 Å |
| 8RRX Structure of RyR1 reconstituted into lipid nanodisc in primed state in complex with Ca2+, ATP, caffeine and Nb9657 Deposited 2024-01-23 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: 12-meric |
Chain A
11–5037（5027 aa）
Chain D
11–5037（5027 aa）
Chain G
11–5037（5027 aa）
Chain I
11–5037（5027 aa）
|
Not recorded | ZN ZINC ION × 4 POV (2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate × 4 ATP ADENOSINE-5'-TRIPHOSPHATE × 4 CFF CAFFEINE × 4 CA CALCIUM ION × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.10 Å |
| 8RS0 Structure of RyR1 in detergent in primed state in complex with nanobody and FKBP Deposited 2024-01-24 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: 12-meric |
Chain B
11–5037（5027 aa）
Chain E
11–5037（5027 aa）
Chain G
11–5037（5027 aa）
Chain J
11–5037（5027 aa）
|
Not recorded | ZN ZINC ION × 4 ATP ADENOSINE-5'-TRIPHOSPHATE × 4 CFF CAFFEINE × 4 CA CALCIUM ION × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.30 Å |
| 8SEN Cryo-EM Structure of RyR1 Deposited 2023-04-10 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Insufficient information Heteromer；Protein × 8 PDB declaration: octameric |
Chain A
1–5037（5037 aa）
Chain B
1–5037（5037 aa）
Chain C
1–5037（5037 aa）
Chain D
1–5037（5037 aa）
|
Not recorded | ZN ZINC ION × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.49 Å |
| 8SEO Cryo-EM Structure of RyR1 + ATP-gamma-S Deposited 2023-04-10 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Insufficient information Heteromer；Protein × 8 PDB declaration: octameric |
Chain A
1–5037（5037 aa）
Chain B
1–5037（5037 aa）
Chain C
1–5037（5037 aa）
Chain D
1–5037（5037 aa）
|
Not recorded | AGS PHOSPHOTHIOPHOSPHORIC ACID-ADENYLATE ESTER × 4 ZN ZINC ION × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.92 Å |
| 8SEP Cryo-EM Structure of RyR1 + ADP Deposited 2023-04-10 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Insufficient information Heteromer；Protein × 8 PDB declaration: octameric |
Chain A
1–5037（5037 aa）
Chain B
1–5037（5037 aa）
Chain C
1–5037（5037 aa）
Chain D
1–5037（5037 aa）
|
Not recorded | ADP ADENOSINE-5'-DIPHOSPHATE × 4 ZN ZINC ION × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.57 Å |
| 8SEQ Cryo-EM Structure of RyR1 + AMP Deposited 2023-04-10 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Insufficient information Heteromer；Protein × 8 PDB declaration: octameric |
Chain A
1–5037（5037 aa）
Chain B
1–5037（5037 aa）
Chain C
1–5037（5037 aa）
Chain D
1–5037（5037 aa）
|
Not recorded | AMP ADENOSINE MONOPHOSPHATE × 4 ZN ZINC ION × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.40 Å |
| 8SER Cryo-EM Structure of RyR1 + Adenosine Deposited 2023-04-10 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Insufficient information Heteromer；Protein × 8 PDB declaration: octameric |
Chain A
1–5037（5037 aa）
Chain B
1–5037（5037 aa）
Chain C
1–5037（5037 aa）
Chain D
1–5037（5037 aa）
|
Not recorded | ADN ADENOSINE × 4 ZN ZINC ION × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.42 Å |
| 8SES Cryo-EM Structure of RyR1 + Adenine Deposited 2023-04-10 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Insufficient information Heteromer；Protein × 8 PDB declaration: octameric |
Chain A
1–5037（5037 aa）
Chain B
1–5037（5037 aa）
Chain C
1–5037（5037 aa）
Chain D
1–5037（5037 aa）
|
Not recorded | ZN ZINC ION × 4 ADE ADENINE × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.98 Å |
| 8SET Cryo-EM Structure of RyR1 + cAMP Deposited 2023-04-10 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Insufficient information Heteromer；Protein × 8 PDB declaration: octameric |
Chain A
1–5037（5037 aa）
Chain B
1–5037（5037 aa）
Chain C
1–5037（5037 aa）
Chain D
1–5037（5037 aa）
|
Not recorded | CMP ADENOSINE-3',5'-CYCLIC-MONOPHOSPHATE × 4 ZN ZINC ION × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.42 Å |
| 8SEU Cryo-EM Structure of RyR1 (Local Refinement of TMD) Deposited 2023-04-10 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric |
Chain A
1–5037（5037 aa）
Chain B
1–5037（5037 aa）
Chain C
1–5037（5037 aa）
Chain D
1–5037（5037 aa）
|
Not recorded | ZN ZINC ION × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.00 Å |
| 8SEV Cryo-EM Structure of RyR1 + ATP-gamma-S (Local Refinement of TMD) Deposited 2023-04-10 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric |
Chain A
1–5037（5037 aa）
Chain B
1–5037（5037 aa）
Chain C
1–5037（5037 aa）
Chain D
1–5037（5037 aa）
|
Not recorded | AGS PHOSPHOTHIOPHOSPHORIC ACID-ADENYLATE ESTER × 4 ZN ZINC ION × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.17 Å |
| 8SEW Cryo-EM Structure of RyR1 + ADP (Local Refinement of TMD) Deposited 2023-04-10 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric |
Chain A
1–5037（5037 aa）
Chain B
1–5037（5037 aa）
Chain C
1–5037（5037 aa）
Chain D
1–5037（5037 aa）
|
Not recorded | ADP ADENOSINE-5'-DIPHOSPHATE × 4 ZN ZINC ION × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.89 Å |
| 8SEX Cryo-EM Structure of RyR1 + AMP (Local Refinement of TMD) Deposited 2023-04-10 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric |
Chain A
1–5037（5037 aa）
Chain B
1–5037（5037 aa）
Chain C
1–5037（5037 aa）
Chain D
1–5037（5037 aa）
|
Not recorded | AMP ADENOSINE MONOPHOSPHATE × 4 ZN ZINC ION × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.84 Å |
| 8SEY Cryo-EM Structure of RyR1 + Adenosine (Local Refinement of TMD) Deposited 2023-04-10 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric |
Chain A
1–5037（5037 aa）
Chain B
1–5037（5037 aa）
Chain C
1–5037（5037 aa）
Chain D
1–5037（5037 aa）
|
Not recorded | ADN ADENOSINE × 4 ZN ZINC ION × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.99 Å |
| 8SEZ Cryo-EM Structure of RyR1 + Adenine (Local Refinement of TMD) Deposited 2023-04-10 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric |
Chain A
1–5037（5037 aa）
Chain B
1–5037（5037 aa）
Chain C
1–5037（5037 aa）
Chain D
1–5037（5037 aa）
|
Not recorded | ADE ADENINE × 4 ZN ZINC ION × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.54 Å |
| 8SF0 Cryo-EM Structure of RyR1 + cAMP (Local Refinement of TMD) Deposited 2023-04-10 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric |
Chain A
1–5037（5037 aa）
Chain B
1–5037（5037 aa）
Chain C
1–5037（5037 aa）
Chain D
1–5037（5037 aa）
|
Not recorded | CMP ADENOSINE-3',5'-CYCLIC-MONOPHOSPHATE × 4 ZN ZINC ION × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.90 Å |
| 8X48 Cryo-EM structure of Ryanodine receptor 1 (EGTA) Deposited 2023-11-15 | Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric |
Chain A
1–5037（5037 aa）
Chain B
1–5037（5037 aa）
Chain C
1–5037（5037 aa）
Chain D
1–5037（5037 aa）
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.00 Å |
| 8X49 Cryo-EM structure of Ryanodine receptor 1 (100 nM Ca2+) Deposited 2023-11-15 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric |
Chain A
1–5037（5037 aa）
Chain B
1–5037（5037 aa）
Chain C
1–5037（5037 aa）
Chain D
1–5037（5037 aa）
|
Not recorded | CA CALCIUM ION × 4 ZN ZINC ION × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.40 Å |
| 8X4A Cryo-EM structure of Ryanodine receptor 1 (TM helix S0,100 nM Ca2+, closed state) Deposited 2023-11-15 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric |
Chain A
1–5037（5037 aa）
Chain B
1–5037（5037 aa）
Chain C
1–5037（5037 aa）
Chain D
1–5037（5037 aa）
|
Not recorded | CA CALCIUM ION × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.80 Å |
| 8X4B Cryo-EM structure of Ryanodine receptor 1 (TM helix S0,100 nM Ca2+, open state) Deposited 2023-11-15 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric |
Chain A
1–5037（5037 aa）
Chain B
1–5037（5037 aa）
Chain C
1–5037（5037 aa）
Chain D
1–5037（5037 aa）
|
Not recorded | CA CALCIUM ION × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.20 Å |
| 8X4C Cryo-EM structure of Ryanodine receptor 1 (20 uM Ca2+, 2 mM ATP) Deposited 2023-11-15 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric |
Chain A
1–5037（5037 aa）
Chain B
1–5037（5037 aa）
Chain C
1–5037（5037 aa）
Chain D
1–5037（5037 aa）
|
Not recorded | ATP ADENOSINE-5'-TRIPHOSPHATE × 4 CA CALCIUM ION × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.80 Å |
| 8X4D Cryo-EM structure of Ryanodine receptor 1 (TM helix S0,20 uM Ca2+, 2 mM ATP) Deposited 2023-11-15 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric |
Chain A
1–5037（5037 aa）
Chain B
1–5037（5037 aa）
Chain C
1–5037（5037 aa）
Chain D
1–5037（5037 aa）
|
Not recorded | CA CALCIUM ION × 4 ATP ADENOSINE-5'-TRIPHOSPHATE × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.90 Å |
| 8X4E Cryo-EM structure of Ryanodine receptor 1 (TM helix S0, 5 mM Ca2+) Deposited 2023-11-15 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric |
Chain A
1–5037（5037 aa）
Chain B
1–5037（5037 aa）
Chain C
1–5037（5037 aa）
Chain D
1–5037（5037 aa）
|
Not recorded | ZN ZINC ION × 4 CA CALCIUM ION × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.30 Å |
| 8XJI Structure of chimeric RyR complex with flubendiamide Deposited 2023-12-21 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric |
Chain A
1–5037（5037 aa）
Chain B
1–5037（5037 aa）
Chain C
1–5037（5037 aa）
Chain D
1–5037（5037 aa）
|
Mutation：R4563K, F4564Y,C4657I, L4792S Mutation：R4563K, F4564Y,C4657I, L4792S Mutation：R4563K, F4564Y,C4657I, L4792S Mutation：R4563K, F4564Y,C4657I, L4792S | A1LVX Flubendiamide × 4 ZN ZINC ION × 4 CA CALCIUM ION × 4 ATP ADENOSINE-5'-TRIPHOSPHATE × 4 CFF CAFFEINE × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.91 Å |
| 8XKH Structure of chimeric RyR Complex with tetraniliprole Deposited 2023-12-23 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric |
Chain A
1–5037（5037 aa）
Chain B
1–5037（5037 aa）
Chain C
1–5037（5037 aa）
Chain D
1–5037（5037 aa）
|
Mutation：R4563K, F4564Y,C4657I, L4792S Mutation：R4563K, F4564Y,C4657I, L4792S Mutation：R4563K, F4564Y,C4657I, L4792S Mutation：R4563K, F4564Y,C4657I, L4792S | ZN ZINC ION × 4 CA CALCIUM ION × 4 ATP ADENOSINE-5'-TRIPHOSPHATE × 4 CFF CAFFEINE × 4 A1LV1 tetraniliprole × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.87 Å |
| 8XLF Structure of chimeric RyR Deposited 2023-12-25 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric |
Chain A
1–5037（5037 aa）
Chain B
1–5037（5037 aa）
Chain C
1–5037（5037 aa）
Chain D
1–5037（5037 aa）
|
Mutation：R4563K, F4564Y,C4657I, L4792S Mutation：R4563K, F4564Y,C4657I, L4792S Mutation：R4563K, F4564Y,C4657I, L4792S Mutation：R4563K, F4564Y,C4657I, L4792S | CA CALCIUM ION × 4 ZN ZINC ION × 4 ATP ADENOSINE-5'-TRIPHOSPHATE × 4 CFF CAFFEINE × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.62 Å |
| 8XLH Structure of chimeric RyR-I4657M/G4819E Deposited 2023-12-26 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric |
Chain A
1–5037（5037 aa）
Chain B
1–5037（5037 aa）
Chain C
1–5037（5037 aa）
Chain D
1–5037（5037 aa）
|
Mutation：R4563K, F4564Y,C4657M, L4792S,G4819E Mutation：R4563K, F4564Y,C4657M, L4792S,G4819E Mutation：R4563K, F4564Y,C4657M, L4792S,G4819E Mutation：R4563K, F4564Y,C4657M, L4792S,G4819E | ZN ZINC ION × 4 CA CALCIUM ION × 4 ATP ADENOSINE-5'-TRIPHOSPHATE × 4 CFF CAFFEINE × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.62 Å |
| 8Y40 Structure of chimeric RyR-I4657M/G4819E complex with chlorantraniliprole Deposited 2024-01-29 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: 12-meric |
Chain A
1–5037（5037 aa）
Chain B
1–5037（5037 aa）
Chain C
1–5037（5037 aa）
Chain D
1–5037（5037 aa）
|
Mutation：R4563K, F4564Y,C4657M, L4792S,G4819E Mutation：R4563K, F4564Y,C4657M, L4792S,G4819E Mutation：R4563K, F4564Y,C4657M, L4792S,G4819E Mutation：R4563K, F4564Y,C4657M, L4792S,G4819E | F0U 5-bromanyl-N-[4-chloranyl-2-methyl-6-(methylcarbamoyl)phenyl]-2-(3-chloranylpyridin-2-yl)pyrazole-3-carboxamide × 4 ZN ZINC ION × 4 CA CALCIUM ION × 4 ATP ADENOSINE-5'-TRIPHOSPHATE × 4 CFF CAFFEINE × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.58 Å |
| 9CGP RyR1 disease mutant Y523S with FKBP12.6, nanodisc and inhibitor dantrolene in the absence of calcium with refined P1 domain Deposited 2024-06-30 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain A
1–5037（5037 aa）
Chain B
1–5037（5037 aa）
Chain C
1–5037（5037 aa）
Chain D
1–5037（5037 aa）
|
Mutation：Y523S Mutation：Y523S Mutation：Y523S Mutation：Y523S | ZN ZINC ION × 4 ATP ADENOSINE-5'-TRIPHOSPHATE × 4 U1C Dantrolene × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.34 Å |
| 9CGQ Rabbit RyR1 disease mutant Y523S in complex with FKBP12.6, nanodisc and inhibitor dantrolene in the presence of activating calcium Deposited 2024-06-30 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain A
1–5037（5037 aa）
Chain B
1–5037（5037 aa）
Chain C
1–5037（5037 aa）
Chain D
1–5037（5037 aa）
|
Mutation：Y523S Mutation：Y523S Mutation：Y523S Mutation：Y523S | ZN ZINC ION × 4 ATP ADENOSINE-5'-TRIPHOSPHATE × 4 CA CALCIUM ION × 4 U1C Dantrolene × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.23 Å |
| 9E17 Structure of RyR1 in the primed state in the presence of caffeine (reprocessed/reanalyzed from EMPIAR-10997, 7TZC, EMD-26205) Deposited 2024-10-21 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: 12-meric |
Chain A
1–5037（5037 aa）
Chain B
1–5037（5037 aa）
Chain G
1–5037（5037 aa）
Chain I
1–5037（5037 aa）
|
Not recorded | CA CALCIUM ION × 20 ATP ADENOSINE-5'-TRIPHOSPHATE × 8 ZN ZINC ION × 4 CFF CAFFEINE × 4 KVR 4-[(7-methoxy-2,3-dihydro-1,4-benzothiazepin-4(5H)-yl)methyl]benzoic acid × 4 L9R (2S)-3-(octadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate × 8 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.45 Å |
| 9E18 Structure of RyR1 in the primed state in the presence of pentoxifylline Deposited 2024-10-21 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain A
1–5037（5037 aa）
Chain B
1–5037（5037 aa）
Chain C
1–5037（5037 aa）
Chain D
1–5037（5037 aa）
|
Not recorded | ATP ADENOSINE-5'-TRIPHOSPHATE × 4 CA CALCIUM ION × 4 ZN ZINC ION × 4 PNX 3,7-DIMETHYL-1-(5-OXOHEXYL)-3,7-DIHYDRO-1H-PURINE-2,6-DIONE × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4；2 mM pentoxifylline was added to the final sample from a 20 mM stock solution in buffer.
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.68 Å |
| 9E19 Structure of RyR1 in the open state in the presence of pentoxifylline Deposited 2024-10-21 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain A
1–5037（5037 aa）
Chain B
1–5037（5037 aa）
Chain C
1–5037（5037 aa）
Chain D
1–5037（5037 aa）
|
Not recorded | ATP ADENOSINE-5'-TRIPHOSPHATE × 4 CA CALCIUM ION × 4 ZN ZINC ION × 4 PNX 3,7-DIMETHYL-1-(5-OXOHEXYL)-3,7-DIHYDRO-1H-PURINE-2,6-DIONE × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4；2 mM pentoxifylline was added to the final sample from a 20 mM stock solution in buffer.
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.04 Å |
| 9E1A Structure of RyR1 in the primed state in the presence of dyphylline Deposited 2024-10-21 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain A
1–5037（5037 aa）
Chain B
1–5037（5037 aa）
Chain C
1–5037（5037 aa）
Chain D
1–5037（5037 aa）
|
Not recorded | ATP ADENOSINE-5'-TRIPHOSPHATE × 4 CA CALCIUM ION × 4 ZN ZINC ION × 4 A1BD2 dyphylline × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4；2 mM dyphylline was added to the final sample from a 20 mM stock solution in buffer.
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.35 Å |
| 9E1B Structure of RyR1 in the open state in the presence of dyphylline Deposited 2024-10-21 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain A
1–5037（5037 aa）
Chain B
1–5037（5037 aa）
Chain C
1–5037（5037 aa）
Chain D
1–5037（5037 aa）
|
Not recorded | ATP ADENOSINE-5'-TRIPHOSPHATE × 4 CA CALCIUM ION × 4 ZN ZINC ION × 4 A1BD2 dyphylline × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4；2 mM dyphylline was added to the final sample from a 20 mM stock solution in buffer.
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.49 Å |
| 9E1C Structure of RyR1 in the primed state in the presence of IBMX Deposited 2024-10-21 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain A
1–5037（5037 aa）
Chain B
1–5037（5037 aa）
Chain C
1–5037（5037 aa）
Chain D
1–5037（5037 aa）
|
Not recorded | ATP ADENOSINE-5'-TRIPHOSPHATE × 4 CA CALCIUM ION × 4 ZN ZINC ION × 4 IBM 3-ISOBUTYL-1-METHYLXANTHINE × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4；2 mM IBMX was added to the final sample from a 100 mM stock solution in 100% DMSO.
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.63 Å |
| 9E1D Structure of RyR1 in the primed state in the presence of enprofylline Deposited 2024-10-21 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain A
1–5037（5037 aa）
Chain B
1–5037（5037 aa）
Chain C
1–5037（5037 aa）
Chain D
1–5037（5037 aa）
|
Not recorded | ATP ADENOSINE-5'-TRIPHOSPHATE × 4 CA CALCIUM ION × 4 ZN ZINC ION × 4 A1BD3 enprofylline × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4；2 mM enprofylline was added to the final sample from a 100 mM stock solution in 100% DMSO.
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.76 Å |
| 9E1E Structure of RyR1 in the primed state in the presence of uracil Deposited 2024-10-21 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain A
1–5037（5037 aa）
Chain B
1–5037（5037 aa）
Chain C
1–5037（5037 aa）
Chain D
1–5037（5037 aa）
|
Not recorded | ATP ADENOSINE-5'-TRIPHOSPHATE × 4 CA CALCIUM ION × 4 ZN ZINC ION × 4 URA URACIL × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4；2 mM uracil was added to the final sample from a 100 mM stock solution in 100% DMSO.
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.92 Å |
| 9E1F Structure of RyR1 in the primed state in the presence of allopurinol Deposited 2024-10-21 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain A
1–5037（5037 aa）
Chain B
1–5037（5037 aa）
Chain C
1–5037（5037 aa）
Chain D
1–5037（5037 aa）
|
Not recorded | ATP ADENOSINE-5'-TRIPHOSPHATE × 4 CA CALCIUM ION × 4 ZN ZINC ION × 4 A1BD4 allopurinol × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4；2 mM allopurinol was added to the final sample from a 100 mM stock solution in 100% DMSO.
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.03 Å |
| 9E1G Structure of RyR1 in the primed state in the presence of oxypurinol Deposited 2024-10-21 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain A
1–5037（5037 aa）
Chain B
1–5037（5037 aa）
Chain C
1–5037（5037 aa）
Chain D
1–5037（5037 aa）
|
Not recorded | ATP ADENOSINE-5'-TRIPHOSPHATE × 4 CA CALCIUM ION × 4 ZN ZINC ION × 4 141 Oxypurinol × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4；2 mM oxypurinol was added to the final sample from a 100 mM stock solution in 100% DMSO.
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.17 Å |
| 9E1H Structure of RyR1 in the primed state in the presence of oxopyricid Deposited 2024-10-21 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain A
1–5037（5037 aa）
Chain B
1–5037（5037 aa）
Chain C
1–5037（5037 aa）
Chain D
1–5037（5037 aa）
|
Not recorded | ATP ADENOSINE-5'-TRIPHOSPHATE × 4 CA CALCIUM ION × 4 ZN ZINC ION × 4 A1BD5 oxopyricid × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4；2 mM oxopyricid was added to the final sample from a 20 mM stock solution in buffer.
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.26 Å |
| 9E1I Structure of RyR1 in the open state in the presence of oxopyricid Deposited 2024-10-21 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain A
1–5037（5037 aa）
Chain B
1–5037（5037 aa）
Chain C
1–5037（5037 aa）
Chain D
1–5037（5037 aa）
|
Not recorded | ATP ADENOSINE-5'-TRIPHOSPHATE × 4 CA CALCIUM ION × 4 ZN ZINC ION × 4 A1BD5 oxopyricid × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4；2 mM oxopyricid was added to the final sample from a 20 mM stock solution in buffer.
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.20 Å |
| 9HEO Open-state RyR1 in 0.05% POPC micelles, in complex with a nanobody and FKBP Deposited 2024-11-14 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: 12-meric |
Chain A
11–5037（5027 aa）
Chain C
11–5037（5027 aa）
Chain G
11–5037（5027 aa）
Chain J
11–5037（5027 aa）
|
Not recorded | ZN ZINC ION × 4 ATP ADENOSINE-5'-TRIPHOSPHATE × 4 CFF CAFFEINE × 4 CA CALCIUM ION × 4 POV (2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate × 72 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.40 Å |
| 9HEQ Open-state RyR1 in 0.01% POPC micelles, in complex with a nanobody and FKBP12 Deposited 2024-11-14 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: 12-meric |
Chain A
11–5037（5027 aa）
Chain C
11–5037（5027 aa）
Chain G
11–5037（5027 aa）
Chain J
11–5037（5027 aa）
|
Not recorded | POV (2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate × 40 ATP ADENOSINE-5'-TRIPHOSPHATE × 4 CFF CAFFEINE × 4 CA CALCIUM ION × 4 ZN ZINC ION × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.60 Å |
| 9OL4 Cryo-EM Structure of Ryanodine Receptor 1: Drug Bound Open Conformation Deposited 2025-05-12 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain A
1–5037（5037 aa）
Chain G
1–5037（5037 aa）
Chain M
1–5037（5037 aa）
Chain S
1–5037（5037 aa）
|
Not recorded | CFF CAFFEINE × 4 CA CALCIUM ION × 4 117 7-[2-(4-FLUORO-PHENYL)-5-ISOPROPYL-3-PHENYL-4-PHENYLCARBAMOYL-PYRROL-1-YL]- 3,5-DIHYDROXY-HEPTANOIC ACID × 12 ZN ZINC ION × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.37 Å |
| 9OL5 Rabbit Ryanodine Receptor 1: Atorvastatin Bound Closed Conformation Deposited 2025-05-12 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain A
1–5037（5037 aa）
Chain G
1–5037（5037 aa）
Chain M
1–5037（5037 aa）
Chain S
1–5037（5037 aa）
|
Not recorded | CA CALCIUM ION × 4 117 7-[2-(4-FLUORO-PHENYL)-5-ISOPROPYL-3-PHENYL-4-PHENYLCARBAMOYL-PYRROL-1-YL]- 3,5-DIHYDROXY-HEPTANOIC ACID × 4 ZN ZINC ION × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.56 Å |
| 9OL6 Rabbit Ryanodine Receptor 1: DMSO Control Closed Conformation Deposited 2025-05-12 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain A
1–5037（5037 aa）
Chain G
1–5037（5037 aa）
Chain M
1–5037（5037 aa）
Chain S
1–5037（5037 aa）
|
Not recorded | CA CALCIUM ION × 4 ZN ZINC ION × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.11 Å |
| 9R8O Primed-state RyR1 in 0.05% POPC micelles, in complex with a nanobody and FKBP Deposited 2025-05-16 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: 12-meric |
Chain C
11–5037（5027 aa）
Chain F
11–5037（5027 aa）
Chain I
11–5037（5027 aa）
Chain J
11–5037（5027 aa）
|
Not recorded | ATP ADENOSINE-5'-TRIPHOSPHATE × 4 CFF CAFFEINE × 4 CA CALCIUM ION × 4 POV (2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate × 40 ZN ZINC ION × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.30 Å |
| 9RCW Primed-state RyR1 in 0.01% POPC micelles, in complex with a nanobody and FKBP12 Deposited 2025-05-30 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: 12-meric |
Chain A
11–5037（5027 aa）
Chain C
11–5037（5027 aa）
Chain G
11–5037（5027 aa）
Chain J
11–5037（5027 aa）
|
Not recorded | ZN ZINC ION × 4 ATP ADENOSINE-5'-TRIPHOSPHATE × 4 CFF CAFFEINE × 4 CA CALCIUM ION × 4 POV (2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate × 24 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.30 Å |
124 other PDB entries and 127 assemblies. Open the comparison page and filter oligomeric states
View Construct and Data Evidence
| UniProt name | RYR1_RABIT |
| Isoform | — |
| PDB entities | 1 |
| Chains and sequence ranges | Author chain A； PDBConstruct 1–197； UniProt 9–205 Author chain B； PDBConstruct 1–197； UniProt 9–205 Author chain C； PDBConstruct 1–197； UniProt 9–205 Author chain D； PDBConstruct 1–197； UniProt 9–205 Author chain E； PDBConstruct 1–197； UniProt 9–205 Author chain F； PDBConstruct 1–197； UniProt 9–205 Author chain G； PDBConstruct 1–197； UniProt 9–205 Author chain H； PDBConstruct 1–197； UniProt 9–205 Author chain I； PDBConstruct 1–197； UniProt 9–205 |
