Ryanodine receptor 1
Oryctolagus cuniculus
State in the Current Structure
| Assembly | Oligomeric State | Construct | Mutations and Modifications | Ligands, Ions and Associated Components | Method and Experimental Conditions | Structure Quality |
|---|---|---|---|---|---|---|
| 1 | Protein homooligomer Homooligomer Protein × 4 PDB declaration: tetrameric（4） Consistent with protein copy count | Chain A； UniProt 1–5037 Chain B； UniProt 1–5037 Chain C； UniProt 1–5037 Chain D； UniProt 1–5037 | Not recorded | ZN ZINC ION × 4 CA CALCIUM ION × 4 | ELECTRON MICROSCOPY cryo-EM buffer：pH 7.4 cryo-EM vitrification conditions：Cryogen ETHANE | Resolution 3.30 Å |
Other States of the Same Protein in the Database
Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.
| Other PDB | Difference from Current Entry 8X4E | Assembly / Oligomeric State | Construct | Mutations and Modifications | Ligands, Ions and Non-polymers | Method and Experimental Conditions | Structure Quality |
|---|---|---|---|---|---|---|---|
| 2BCX Crystal structure of calmodulin in complex with a ryanodine receptor peptide Deposited 2005-10-19 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain B
3614–3643（30 aa）
Fragment：residues 3614-3643
|
Not recorded | CA CALCIUM ION × 4 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 4.7；293 K；23% PEG 4000, 0.1 M sodium acetate, 5 mM DTT, pH 4.7, VAPOR DIFFUSION, SITTING DROP, temperature 293K
|
Resolution 2.00 Å R-free 0.249 |
| 2XOA Crystal Structure of the N-terminal three domains of the skeletal muscle Ryanodine Receptor (RyR1) Deposited 2010-08-10 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–559（559 aa）
Fragment：N-TERMINAL DISEASE HOT SPOT, RESIDUES 1-559
|
Not recorded | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 8；pH 8
|
Resolution 2.50 Å R-free 0.234 |
| 3HSM Crystal structure of distal N-terminal beta-trefoil domain of Ryanodine Receptor type 1 Deposited 2009-06-10 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–210（210 aa）
Fragment：sequence database residues 1-210
|
Not recorded | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8；298 K；0.1M MES, 0.1M MgCl2, 24% PEG 3350, 5mM DTT, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 2.50 Å R-free 0.276 |
| 3HSM Crystal structure of distal N-terminal beta-trefoil domain of Ryanodine Receptor type 1 Deposited 2009-06-10 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
1–210（210 aa）
Fragment：sequence database residues 1-210
|
Not recorded | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8；298 K；0.1M MES, 0.1M MgCl2, 24% PEG 3350, 5mM DTT, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 2.50 Å R-free 0.276 |
| 3ILA Crystal structure of rabbit ryanodine receptor 1 N-terminal domain (9-205) Deposited 2009-08-06 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
9–205（197 aa）
Fragment：N-Terminal Domain
|
Not recorded | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；295 K；15-25% PEG 3350, 0.2M sodium chloride, 0.1M BisTris, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K
|
Resolution 2.90 Å R-free 0.320 |
| 3ILA Crystal structure of rabbit ryanodine receptor 1 N-terminal domain (9-205) Deposited 2009-08-06 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
9–205（197 aa）
Fragment：N-Terminal Domain
|
Not recorded | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；295 K；15-25% PEG 3350, 0.2M sodium chloride, 0.1M BisTris, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K
|
Resolution 2.90 Å R-free 0.320 |
| 3ILA Crystal structure of rabbit ryanodine receptor 1 N-terminal domain (9-205) Deposited 2009-08-06 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain C
9–205（197 aa）
Fragment：N-Terminal Domain
|
Not recorded | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；295 K；15-25% PEG 3350, 0.2M sodium chloride, 0.1M BisTris, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K
|
Resolution 2.90 Å R-free 0.320 |
| 3ILA Crystal structure of rabbit ryanodine receptor 1 N-terminal domain (9-205) Deposited 2009-08-06 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 4 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain D
9–205（197 aa）
Fragment：N-Terminal Domain
|
Not recorded | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；295 K；15-25% PEG 3350, 0.2M sodium chloride, 0.1M BisTris, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K
|
Resolution 2.90 Å R-free 0.320 |
| 3ILA Crystal structure of rabbit ryanodine receptor 1 N-terminal domain (9-205) Deposited 2009-08-06 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 5 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain E
9–205（197 aa）
Fragment：N-Terminal Domain
|
Not recorded | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；295 K；15-25% PEG 3350, 0.2M sodium chloride, 0.1M BisTris, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K
|
Resolution 2.90 Å R-free 0.320 |
| 3ILA Crystal structure of rabbit ryanodine receptor 1 N-terminal domain (9-205) Deposited 2009-08-06 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 6 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain F
9–205（197 aa）
Fragment：N-Terminal Domain
|
Not recorded | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；295 K；15-25% PEG 3350, 0.2M sodium chloride, 0.1M BisTris, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K
|
Resolution 2.90 Å R-free 0.320 |
| 3ILA Crystal structure of rabbit ryanodine receptor 1 N-terminal domain (9-205) Deposited 2009-08-06 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 7 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain G
9–205（197 aa）
Fragment：N-Terminal Domain
|
Not recorded | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；295 K；15-25% PEG 3350, 0.2M sodium chloride, 0.1M BisTris, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K
|
Resolution 2.90 Å R-free 0.320 |
| 3ILA Crystal structure of rabbit ryanodine receptor 1 N-terminal domain (9-205) Deposited 2009-08-06 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 8 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain H
9–205（197 aa）
Fragment：N-Terminal Domain
|
Not recorded | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；295 K；15-25% PEG 3350, 0.2M sodium chloride, 0.1M BisTris, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K
|
Resolution 2.90 Å R-free 0.320 |
| 3ILA Crystal structure of rabbit ryanodine receptor 1 N-terminal domain (9-205) Deposited 2009-08-06 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 9 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain I
9–205（197 aa）
Fragment：N-Terminal Domain
|
Not recorded | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；295 K；15-25% PEG 3350, 0.2M sodium chloride, 0.1M BisTris, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K
|
Resolution 2.90 Å R-free 0.320 |
| 3J8H Structure of the rabbit ryanodine receptor RyR1 in complex with FKBP12 at 3.8 Angstrom resolution Deposited 2014-10-26 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain A
1–1491（1491 aa）
Chain A
1510–1976（467 aa）
Chain A
2057–2460（404 aa）
Chain A
2494–2562（69 aa）
Chain A
2734–2940（207 aa）
Chain A
3645–5037（1393 aa）
Chain C
1–1491（1491 aa）
Chain C
1510–1976（467 aa）
Chain C
2057–2460（404 aa）
Chain C
2494–2562（69 aa）
Chain C
2734–2940（207 aa）
Chain C
3645–5037（1393 aa）
Chain E
1–1491（1491 aa）
Chain E
1510–1976（467 aa）
Chain E
2057–2460（404 aa）
Chain E
2494–2562（69 aa）
Chain E
2734–2940（207 aa）
Chain E
3645–5037（1393 aa）
Chain G
1–1491（1491 aa）
Chain G
1510–1976（467 aa）
Chain G
2057–2460（404 aa）
Chain G
2494–2562（69 aa）
Chain G
2734–2940（207 aa）
Chain G
3645–5037（1393 aa）
|
Not recorded | ZN ZINC ION × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
20 mM MOPS sodium, pH 7.4, 250 mM NaCl, 2 mM DTT, 2 mM EGTA, 0.015% Tween20, protein inhibitors；pH 7.4；20 mM MOPS sodium, pH 7.4, 250 mM NaCl, 2 mM DTT, 2 mM EGTA, 0.015% Tween20, protein inhibitors
cryo-EM vitrification conditions
protein solution was applied to glow-discharged carbon coated grid and blotted for 2 seconds before plunging；Cryogen NITROGEN；Protein solution was applied to glow-discharged carbon coated grid and blotted for 2 seconds before plunging into liquid nitrogen (FEI VITROBOT MARK III)
|
Resolution 3.80 Å |
| 3RQR Crystal structure of the RYR domain of the rabbit ryanodine receptor Deposited 2011-04-28 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain A
2733–2940（208 aa）
Fragment：Residues 2733-2940
|
Not recorded | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 8.5；291 K；1.2M Na Citrate, 100mM Tris pH8.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K
|
Resolution 2.16 Å R-free 0.258 |
| 4ERT Crystal structure of rabbit ryanodine receptor 1 (2734-2940) Deposited 2012-04-20 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
2734–2940（207 aa）
|
Not recorded | GOL GLYCEROL × 2 K POTASSIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；295 K；19% PEG3350, 30% Glycerol, 0.1M HEPES, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K
|
Resolution 1.95 Å R-free 0.225 |
| 4ESU Crystal structure of rabbit ryanodine receptor 1 mutant S2776M Deposited 2012-04-23 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
2734–2940（207 aa）
|
Mutation：S2776M | GOL GLYCEROL × 5 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；295 K；25% PEG3350, 25% Glycerol, 0.1M HEPES, pH 7.0, vapor diffusion, hanging drop, temperature 295K
|
Resolution 1.59 Å R-free 0.229 |
| 4ETT Crystal structure of rabbit ryanodine receptor 1 mutant E2764K Deposited 2012-04-24 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
2734–2940（207 aa）
|
Mutation：E2764K | GOL GLYCEROL × 4 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；295 K；25% PEG3350, 25% Glycerol, 0.1M HEPES, pH 7.0, vapor diffusion, hanging drop, temperature 295K
|
Resolution 2.19 Å R-free 0.287 |
| 4ETU Crystal structure of rabbit ryanodine receptor 1 mutant R2939S Deposited 2012-04-24 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
2734–2940（207 aa）
|
Mutation：R2939S | GOL GLYCEROL × 3 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；295 K；15% PEG3350, 25% Glycerol, 0.1M HEPES, pH 7.0, vapor diffusion, hanging drop, temperature 295K
|
Resolution 2.19 Å R-free 0.262 |
| 4I0Y CRYSTAL STRUCTURE OF RABBIT RYANODINE RECEPTOR 1 (RESIDUES 1-536) DISEASE MUTANT C36R Deposited 2012-11-19 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–536（536 aa）
Fragment：N-TERMINAL DISEASE HOT SPOT, RESIDUES 1-536
|
Mutation：C36R | GOL GLYCEROL × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
EVAPORATION；pH 9.5；pH 9.5, EVAPORATION
|
Resolution 2.80 Å R-free 0.246 |
| 4I1E Crystal structure of Rabbit Ryanodine Receptor 1 (residues 1-536) disease mutant G249R Deposited 2012-11-20 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–536（536 aa）
Fragment：N-TERMINAL DISEASE HOT SPOT, RESIDUES 1-536
|
Mutation：G249R | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
EVAPORATION；pH 8；pH 8.0, EVAPORATION
|
Resolution 2.40 Å R-free 0.258 |
| 4I2S Crystal structure of rabbit ryanodine receptor 1 (residues 1-536) disease mutant I404M Deposited 2012-11-22 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–536（536 aa）
Fragment：N-TERMINAL DISEASE HOT SPOT, RESIDUES 1-536
|
Mutation：I404M | GOL GLYCEROL × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
EVAPORATION；pH 8；pH 8.0, EVAPORATION
|
Resolution 2.50 Å R-free 0.249 |
| 4I37 Crystal structure of rabbit ryanodine receptor 1 (residues 1-536) disease mutant R402G Deposited 2012-11-25 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–536（536 aa）
Fragment：N-TERMINAL DISEASE HOT SPOT, RESIDUES 1-536
|
Mutation：R402G | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
EVAPORATION；pH 6.5；pH 6.5, EVAPORATION
|
Resolution 2.95 Å R-free 0.289 |
| 4I3N Crystal structure of rabbit ryanodine receptor 1 (residues 1-536) disease mutant D61N Deposited 2012-11-26 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–536（536 aa）
Fragment：N-TERMINAL DISEASE HOT SPOT, RESIDUES 1-536
|
Mutation：D61N | GOL GLYCEROL × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
EVAPORATION；pH 9.5；pH 9.5, EVAPORATION
|
Resolution 2.95 Å R-free 0.257 |
| 4I6I Crystal structure of rabbit ryanodine receptor 1 (residues 1-559) disease mutant R45C Deposited 2012-11-29 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–559（559 aa）
Fragment：N-TERMINAL DISEASE HOT SPOT, RESIDUES 1-559
|
Mutation：R45C | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
EVAPORATION；pH 7；pH 7.0, EVAPORATION
|
Resolution 2.50 Å R-free 0.277 |
| 4I7I Crystal structure of rabbit ryanodine receptor 1 (residues 1-536) disease mutant L14R Deposited 2012-11-30 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–536（536 aa）
Fragment：N-TERMINAL DISEASE HOT SPOT, RESIDUES 1-536
|
Mutation：L14R | GOL GLYCEROL × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
EVAPORATION；pH 8；pH 8.0, EVAPORATION
|
Resolution 2.90 Å R-free 0.249 |
| 4I8M Crystal structure of rabbit Ryanodine receptor 1 (residues 1-536) disease mutant V219I Deposited 2012-12-03 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–536（536 aa）
Fragment：N-TERMINAL DISEASE HOT SPOT, RESIDUES 1-536
|
Mutation：V219I | GOL GLYCEROL × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
EVAPORATION；pH 9；pH 9.0, EVAPORATION
|
Resolution 2.80 Å R-free 0.253 |
| 4I96 Crystal structure of the N-terminal two domains of the skeletal muscle ryanodine receptor (rabbit RyR1) residues 217-536 Deposited 2012-12-04 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
217–536（320 aa）
Fragment：N-TERMINAL DISEASE HOT SPOT, RESIDUES 217-536
|
Not recorded | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
EVAPORATION；pH 8；pH 8.0, EVAPORATION
|
Resolution 2.73 Å R-free 0.241 |
| 4P9J Crystal Structure of rabbit Ryanodine Receptor 1 SPRY2 Domain (1070-1246) Deposited 2014-04-04 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain C
1070–1246（177 aa）
Fragment：UNP Residues 1070-1246
|
Not recorded | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；298.15 K；sodium citrate
|
Resolution 1.84 Å R-free 0.220 |
| 4P9J Crystal Structure of rabbit Ryanodine Receptor 1 SPRY2 Domain (1070-1246) Deposited 2014-04-04 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1070–1246（177 aa）
Fragment：UNP Residues 1070-1246
|
Not recorded | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；298.15 K；sodium citrate
|
Resolution 1.84 Å R-free 0.220 |
| 4P9J Crystal Structure of rabbit Ryanodine Receptor 1 SPRY2 Domain (1070-1246) Deposited 2014-04-04 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
1070–1246（177 aa）
Fragment：UNP Residues 1070-1246
|
Not recorded | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；298.15 K；sodium citrate
|
Resolution 1.84 Å R-free 0.220 |
| 4UWA Structure of the ryanodine receptor at resolution of 6.1 A in closed state Deposited 2014-08-11 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric |
Chain A
1–533（533 aa）
Chain A
649–1571（923 aa）
Chain A
2734–2939（206 aa）
Chain B
1–533（533 aa）
Chain B
649–1571（923 aa）
Chain B
2734–2939（206 aa）
Chain C
1–533（533 aa）
Chain C
649–1571（923 aa）
Chain C
2734–2939（206 aa）
Chain D
1–533（533 aa）
Chain D
649–1571（923 aa）
Chain D
2734–2939（206 aa）
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
10 MM MOPS, 200MM NACL, 2MM DTT, 1MM EGTA；pH 7.4；10 MM MOPS, 200MM NACL, 2MM DTT, 1MM EGTA
cryo-EM vitrification conditions
Cryogen ETHANE；LIQUID ETHANE
|
Resolution 6.10 Å |
| 4UWE Structure of the ryanodine receptor at resolution of 8.5 A in partially open state Deposited 2014-08-11 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric |
Chain A
1–533（533 aa）
Chain A
649–1571（923 aa）
Chain A
2734–2939（206 aa）
Chain B
1–533（533 aa）
Chain B
649–1571（923 aa）
Chain B
2734–2939（206 aa）
Chain C
1–533（533 aa）
Chain C
649–1571（923 aa）
Chain C
2734–2939（206 aa）
Chain D
1–533（533 aa）
Chain D
649–1571（923 aa）
Chain D
2734–2939（206 aa）
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
10 MM MOPS, 200 MM NACL, 2 MM DTT AND 10 MM CACL2, 0.2% FLUORINATED OCTYL- MALTOSIDE；pH 7.4；10 MM MOPS, 200 MM NACL, 2 MM DTT AND 10 MM CACL2, 0.2% FLUORINATED OCTYL- MALTOSIDE
cryo-EM vitrification conditions
Cryogen ETHANE；LIQUID ETHANE
|
Resolution 8.50 Å |
| 5C30 Crystal Structure of Rabbit Ryanodine Receptor 1 Repeat12 Domain Deposited 2015-06-16 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
857–1054（198 aa）
Fragment：repeat12 domain (UNP residues 857-1054)
|
Mutation：C906A, C937A, C1040A | IPA ISOPROPYL ALCOHOL × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；277 K；0.1 M HEPES, pH 7.0, 19% w/v PEG20000
|
Resolution 1.55 Å R-free 0.165 |
| 5GKY Structure of RyR1 in a closed state (C1 conformer) Deposited 2016-07-07 | Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain A
1–5037（5037 aa）
Chain C
1–5037（5037 aa）
Chain E
1–5037（5037 aa）
Chain G
1–5037（5037 aa）
|
Not recorded | ZN ZINC ION × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4；20 mM MOPS-Na, pH 7.4, 250 mM NaCl, 2 mM DTT, 0.015% Tween20 (w/v) (Sigma-Aldrich) and protease inhibitor cocktail including 2 mM PMSF, 2.6 ug/ml aprotinin, 1.4 ug/ml pepstatin, and 10 ug/ml leupeptin (Amresco)
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.80 Å |
| 5GKZ Structure of RyR1 in a closed state (C3 conformer) Deposited 2016-07-07 | Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain A
1–5037（5037 aa）
Chain C
1–5037（5037 aa）
Chain E
1–5037（5037 aa）
Chain G
1–5037（5037 aa）
|
Not recorded | ZN ZINC ION × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4；20 mM MOPS-Na, pH 7.4, 250 mM NaCl, 2 mM DTT, 0.015% Tween20 (w/v) (Sigma-Aldrich) and protease inhibitor cocktail including 2 mM PMSF, 2.6 ug/ml aprotinin, 1.4 ug/ml pepstatin, and 10 ug/ml leupeptin (Amresco)
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.00 Å |
| 5GL0 Structure of RyR1 in a closed state (C4 conformer) Deposited 2016-07-07 | Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain A
1–5037（5037 aa）
Chain C
1–5037（5037 aa）
Chain E
1–5037（5037 aa）
Chain G
1–5037（5037 aa）
|
Not recorded | ZN ZINC ION × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4；20 mM MOPS-Na, pH 7.4, 250 mM NaCl, 2 mM DTT, 0.015% Tween20 (w/v) (Sigma-Aldrich) and protease inhibitor cocktail including 2 mM PMSF, 2.6 ug/ml aprotinin, 1.4 ug/ml pepstatin, and 10 ug/ml leupeptin (Amresco)
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.20 Å |
| 5GL1 Structure of RyR1 in an open state Deposited 2016-07-07 | Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain A
1–5037（5037 aa）
Chain C
1–5037（5037 aa）
Chain E
1–5037（5037 aa）
Chain G
1–5037（5037 aa）
|
Not recorded | ZN ZINC ION × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4；20 mM MOPS-Na, pH 7.4, 250 mM NaCl, 2 mM DTT, 0.5% CHAPS (w/v) (Sigma-Aldrich) and protease inhibitor cocktail including 2 mM PMSF, 2.6 ug/ml aprotinin, 1.4 ug/ml pepstatin, and 10 ug/ml leupeptin (Amresco),50 uM CaCl2 and 10 uM PCB95
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 5.70 Å |
| 5J8V Structure of rabbit ryanodine receptor RyR1 open state activated by calcium ion Deposited 2016-04-08 | Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric |
Chain A
1–5037（5037 aa）
Chain B
1–5037（5037 aa）
Chain C
1–5037（5037 aa）
Chain D
1–5037（5037 aa）
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4；1:1000 diluted protease inhibitor cocktail was also added in buffer
cryo-EM vitrification conditions
Cryogen ETHANE；Grids were blotted for 2s before plunging.
|
Resolution 4.90 Å |
| 5T15 Structural basis for gating and activation of RyR1 (30 uM Ca2+ dataset, all particles) Deposited 2016-08-17 | Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain B
12–1275（1264 aa）
Chain B
1573–2479（907 aa）
Chain B
2734–2939（206 aa）
Chain B
3639–5037（1399 aa）
Chain E
12–1275（1264 aa）
Chain E
1573–2479（907 aa）
Chain E
2734–2939（206 aa）
Chain E
3639–5037（1399 aa）
Chain G
12–1275（1264 aa）
Chain G
1573–2479（907 aa）
Chain G
2734–2939（206 aa）
Chain G
3639–5037（1399 aa）
Chain I
12–1275（1264 aa）
Chain I
1573–2479（907 aa）
Chain I
2734–2939（206 aa）
Chain I
3639–5037（1399 aa）
|
Not recorded | ZN ZINC ION × 4 CA CALCIUM ION × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE；Blotted for 3-4 seconds on both sides with Whatman ashless filter paper, blot force 3, wait time 30 seconds
|
Resolution 3.60 Å |
| 5T9M Structure of rabbit RyR1 (Ca2+-only dataset, class 1) Deposited 2016-09-09 | Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain B
12–1275（1264 aa）
Chain B
1573–2479（907 aa）
Chain B
2734–2939（206 aa）
Chain B
3639–5037（1399 aa）
Chain E
12–1275（1264 aa）
Chain E
1573–2479（907 aa）
Chain E
2734–2939（206 aa）
Chain E
3639–5037（1399 aa）
Chain G
12–1275（1264 aa）
Chain G
1573–2479（907 aa）
Chain G
2734–2939（206 aa）
Chain G
3639–5037（1399 aa）
Chain I
12–1275（1264 aa）
Chain I
1573–2479（907 aa）
Chain I
2734–2939（206 aa）
Chain I
3639–5037（1399 aa）
|
Not recorded | ZN ZINC ION × 4 CA CALCIUM ION × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE；Blotted for 3-4 seconds on both sides with Whatman ashless filter paper, blot force 3, wait time 30 seconds
|
Resolution 4.00 Å |
| 5T9N Structure of rabbit RyR1 (Ca2+-only dataset, class 2) Deposited 2016-09-09 | Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain B
12–1275（1264 aa）
Chain B
1573–2479（907 aa）
Chain B
2734–2939（206 aa）
Chain B
3639–5037（1399 aa）
Chain E
12–1275（1264 aa）
Chain E
1573–2479（907 aa）
Chain E
2734–2939（206 aa）
Chain E
3639–5037（1399 aa）
Chain G
12–1275（1264 aa）
Chain G
1573–2479（907 aa）
Chain G
2734–2939（206 aa）
Chain G
3639–5037（1399 aa）
Chain I
12–1275（1264 aa）
Chain I
1573–2479（907 aa）
Chain I
2734–2939（206 aa）
Chain I
3639–5037（1399 aa）
|
Not recorded | ZN ZINC ION × 4 CA CALCIUM ION × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE；Blotted for 3-4 seconds on both sides with Whatman ashless filter paper, blot force 3, wait time 30 seconds
|
Resolution 3.80 Å |
| 5T9R Structure of rabbit RyR1 (Ca2+-only dataset, class 3) Deposited 2016-09-09 | Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain B
12–1275（1264 aa）
Chain B
1573–2479（907 aa）
Chain B
2734–2939（206 aa）
Chain B
3639–5037（1399 aa）
Chain E
12–1275（1264 aa）
Chain E
1573–2479（907 aa）
Chain E
2734–2939（206 aa）
Chain E
3639–5037（1399 aa）
Chain G
12–1275（1264 aa）
Chain G
1573–2479（907 aa）
Chain G
2734–2939（206 aa）
Chain G
3639–5037（1399 aa）
Chain I
12–1275（1264 aa）
Chain I
1573–2479（907 aa）
Chain I
2734–2939（206 aa）
Chain I
3639–5037（1399 aa）
|
Not recorded | ZN ZINC ION × 4 CA CALCIUM ION × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE；Blotted for 3-4 seconds on both sides with Whatman ashless filter paper, blot force 3, wait time 30 seconds
|
Resolution 5.80 Å |
| 5T9S Structure of rabbit RyR1 (Ca2+-only dataset, class 4) Deposited 2016-09-09 | Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain B
12–1275（1264 aa）
Chain B
1573–2479（907 aa）
Chain B
2734–2939（206 aa）
Chain B
3639–5037（1399 aa）
Chain E
12–1275（1264 aa）
Chain E
1573–2479（907 aa）
Chain E
2734–2939（206 aa）
Chain E
3639–5037（1399 aa）
Chain G
12–1275（1264 aa）
Chain G
1573–2479（907 aa）
Chain G
2734–2939（206 aa）
Chain G
3639–5037（1399 aa）
Chain I
12–1275（1264 aa）
Chain I
1573–2479（907 aa）
Chain I
2734–2939（206 aa）
Chain I
3639–5037（1399 aa）
|
Not recorded | ZN ZINC ION × 4 CA CALCIUM ION × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE；Blotted for 3-4 seconds on both sides with Whatman ashless filter paper, blot force 3, wait time 30 seconds
|
Resolution 4.20 Å |
| 5T9V Structure of rabbit RyR1 (Caffeine/ATP/Ca2+ dataset, class 1) Deposited 2016-09-09 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain B
12–1275（1264 aa）
Chain B
1573–2479（907 aa）
Chain B
2734–2939（206 aa）
Chain B
3639–4253（615 aa）
Chain B
4540–5037（498 aa）
Chain E
12–1275（1264 aa）
Chain E
1573–2479（907 aa）
Chain E
2734–2939（206 aa）
Chain E
3639–4253（615 aa）
Chain E
4540–5037（498 aa）
Chain G
12–1275（1264 aa）
Chain G
1573–2479（907 aa）
Chain G
2734–2939（206 aa）
Chain G
3639–4253（615 aa）
Chain G
4540–5037（498 aa）
Chain I
12–1275（1264 aa）
Chain I
1573–2479（907 aa）
Chain I
2734–2939（206 aa）
Chain I
3639–4253（615 aa）
Chain I
4540–5037（498 aa）
|
Not recorded | ATP ADENOSINE-5'-TRIPHOSPHATE × 4 CFF CAFFEINE × 4 ZN ZINC ION × 4 CA CALCIUM ION × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE；Blotted for 3-4 seconds on both sides with Whatman ashless filter paper, blot force 3, wait time 30 seconds
|
Resolution 4.40 Å |
| 5TA3 Structure of rabbit RyR1 (Caffeine/ATP/Ca2+ dataset, class 2) Deposited 2016-09-09 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain B
12–1275（1264 aa）
Chain B
1573–2479（907 aa）
Chain B
2734–2939（206 aa）
Chain B
3639–4253（615 aa）
Chain B
4541–5037（497 aa）
Chain E
12–1275（1264 aa）
Chain E
1573–2479（907 aa）
Chain E
2734–2939（206 aa）
Chain E
3639–4253（615 aa）
Chain E
4541–5037（497 aa）
Chain G
12–1275（1264 aa）
Chain G
1573–2479（907 aa）
Chain G
2734–2939（206 aa）
Chain G
3639–4253（615 aa）
Chain G
4541–5037（497 aa）
Chain I
12–1275（1264 aa）
Chain I
1573–2479（907 aa）
Chain I
2734–2939（206 aa）
Chain I
3639–4253（615 aa）
Chain I
4541–5037（497 aa）
|
Not recorded | ATP ADENOSINE-5'-TRIPHOSPHATE × 4 CFF CAFFEINE × 4 ZN ZINC ION × 4 CA CALCIUM ION × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE；Blotted for 3-4 seconds on both sides with Whatman ashless filter paper, blot force 3, wait time 30 seconds
|
Resolution 4.40 Å |
| 5TAL Structure of rabbit RyR1 (Caffeine/ATP/Ca2+ dataset, class 1&2) Deposited 2016-09-10 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain B
12–1275（1264 aa）
Chain B
1573–2479（907 aa）
Chain B
2734–2939（206 aa）
Chain B
3639–4253（615 aa）
Chain B
4541–5037（497 aa）
Chain E
12–1275（1264 aa）
Chain E
1573–2479（907 aa）
Chain E
2734–2939（206 aa）
Chain E
3639–4253（615 aa）
Chain E
4541–5037（497 aa）
Chain G
12–1275（1264 aa）
Chain G
1573–2479（907 aa）
Chain G
2734–2939（206 aa）
Chain G
3639–4253（615 aa）
Chain G
4541–5037（497 aa）
Chain I
12–1275（1264 aa）
Chain I
1573–2479（907 aa）
Chain I
2734–2939（206 aa）
Chain I
3639–4253（615 aa）
Chain I
4541–5037（497 aa）
|
Not recorded | ATP ADENOSINE-5'-TRIPHOSPHATE × 4 CFF CAFFEINE × 4 ZN ZINC ION × 4 CA CALCIUM ION × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE；Blotted for 3-4 seconds on both sides with Whatman ashless filter paper, blot force 3, wait time 30 seconds
|
Resolution 4.30 Å |
| 5TAM Structure of rabbit RyR1 (Caffeine/ATP/Ca2+ dataset, class 4) Deposited 2016-09-10 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain B
12–1275（1264 aa）
Chain B
1573–2479（907 aa）
Chain B
2734–2939（206 aa）
Chain B
3639–4253（615 aa）
Chain B
4541–5037（497 aa）
Chain E
12–1275（1264 aa）
Chain E
1573–2479（907 aa）
Chain E
2734–2939（206 aa）
Chain E
3639–4253（615 aa）
Chain E
4541–5037（497 aa）
Chain G
12–1275（1264 aa）
Chain G
1573–2479（907 aa）
Chain G
2734–2939（206 aa）
Chain G
3639–4253（615 aa）
Chain G
4541–5037（497 aa）
Chain I
12–1275（1264 aa）
Chain I
1573–2479（907 aa）
Chain I
2734–2939（206 aa）
Chain I
3639–4253（615 aa）
Chain I
4541–5037（497 aa）
|
Not recorded | ATP ADENOSINE-5'-TRIPHOSPHATE × 4 CFF CAFFEINE × 4 ZN ZINC ION × 4 CA CALCIUM ION × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE；Blotted for 3-4 seconds on both sides with Whatman ashless filter paper, blot force 3, wait time 30 seconds
|
Resolution 4.30 Å |
| 5TAN Structure of rabbit RyR1 (Caffeine/ATP/Ca2+ dataset, class 3) Deposited 2016-09-10 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain B
12–1275（1264 aa）
Chain B
1573–2479（907 aa）
Chain B
2734–2939（206 aa）
Chain B
3639–4253（615 aa）
Chain B
4541–5037（497 aa）
Chain E
12–1275（1264 aa）
Chain E
1573–2479（907 aa）
Chain E
2734–2939（206 aa）
Chain E
3639–4253（615 aa）
Chain E
4541–5037（497 aa）
Chain G
12–1275（1264 aa）
Chain G
1573–2479（907 aa）
Chain G
2734–2939（206 aa）
Chain G
3639–4253（615 aa）
Chain G
4541–5037（497 aa）
Chain I
12–1275（1264 aa）
Chain I
1573–2479（907 aa）
Chain I
2734–2939（206 aa）
Chain I
3639–4253（615 aa）
Chain I
4541–5037（497 aa）
|
Not recorded | ATP ADENOSINE-5'-TRIPHOSPHATE × 4 CFF CAFFEINE × 4 ZN ZINC ION × 4 CA CALCIUM ION × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE；Blotted for 3-4 seconds on both sides with Whatman ashless filter paper, blot force 3, wait time 30 seconds
|
Resolution 4.30 Å |
| 5TAP Structure of rabbit RyR1 (Caffeine/ATP/EGTA dataset, all particles) Deposited 2016-09-10 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain B
12–1275（1264 aa）
Chain B
1573–2479（907 aa）
Chain B
2734–2939（206 aa）
Chain B
3639–4253（615 aa）
Chain B
4541–5037（497 aa）
Chain E
12–1275（1264 aa）
Chain E
1573–2479（907 aa）
Chain E
2734–2939（206 aa）
Chain E
3639–4253（615 aa）
Chain E
4541–5037（497 aa）
Chain G
12–1275（1264 aa）
Chain G
1573–2479（907 aa）
Chain G
2734–2939（206 aa）
Chain G
3639–4253（615 aa）
Chain G
4541–5037（497 aa）
Chain I
12–1275（1264 aa）
Chain I
1573–2479（907 aa）
Chain I
2734–2939（206 aa）
Chain I
3639–4253（615 aa）
Chain I
4541–5037（497 aa）
|
Not recorded | ATP ADENOSINE-5'-TRIPHOSPHATE × 4 CFF CAFFEINE × 4 ZN ZINC ION × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE；Blotted for 3-4 seconds on both sides with Whatman ashless filter paper, blot force 3, wait time 30 seconds
|
Resolution 4.40 Å |
| 5TAQ Structure of rabbit RyR1 (Caffeine/ATP/Ca2+ dataset, class 3&4) Deposited 2016-09-10 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain B
12–1275（1264 aa）
Chain B
1573–2479（907 aa）
Chain B
2734–2939（206 aa）
Chain B
3639–4253（615 aa）
Chain B
4541–5037（497 aa）
Chain E
12–1275（1264 aa）
Chain E
1573–2479（907 aa）
Chain E
2734–2939（206 aa）
Chain E
3639–4253（615 aa）
Chain E
4541–5037（497 aa）
Chain G
12–1275（1264 aa）
Chain G
1573–2479（907 aa）
Chain G
2734–2939（206 aa）
Chain G
3639–4253（615 aa）
Chain G
4541–5037（497 aa）
Chain I
12–1275（1264 aa）
Chain I
1573–2479（907 aa）
Chain I
2734–2939（206 aa）
Chain I
3639–4253（615 aa）
Chain I
4541–5037（497 aa）
|
Not recorded | ATP ADENOSINE-5'-TRIPHOSPHATE × 4 CFF CAFFEINE × 4 ZN ZINC ION × 4 CA CALCIUM ION × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE；Blotted for 3-4 seconds on both sides with Whatman ashless filter paper, blot force 3, wait time 30 seconds
|
Resolution 4.10 Å |
| 5TAS Structure of rabbit RyR1 (Caffeine/ATP/EGTA dataset, class 1) Deposited 2016-09-10 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain B
12–1275（1264 aa）
Chain B
1573–2479（907 aa）
Chain B
2734–2939（206 aa）
Chain B
3639–4253（615 aa）
Chain B
4541–5037（497 aa）
Chain E
12–1275（1264 aa）
Chain E
1573–2479（907 aa）
Chain E
2734–2939（206 aa）
Chain E
3639–4253（615 aa）
Chain E
4541–5037（497 aa）
Chain G
12–1275（1264 aa）
Chain G
1573–2479（907 aa）
Chain G
2734–2939（206 aa）
Chain G
3639–4253（615 aa）
Chain G
4541–5037（497 aa）
Chain I
12–1275（1264 aa）
Chain I
1573–2479（907 aa）
Chain I
2734–2939（206 aa）
Chain I
3639–4253（615 aa）
Chain I
4541–5037（497 aa）
|
Not recorded | ATP ADENOSINE-5'-TRIPHOSPHATE × 4 CFF CAFFEINE × 4 ZN ZINC ION × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE；Blotted for 3-4 seconds on both sides with Whatman ashless filter paper, blot force 3, wait time 30 seconds
|
Resolution 6.20 Å |
| 5TAT Structure of rabbit RyR1 (Caffeine/ATP/EGTA dataset, class 2) Deposited 2016-09-10 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain B
12–1275（1264 aa）
Chain B
1573–2479（907 aa）
Chain B
2734–2939（206 aa）
Chain B
3639–4253（615 aa）
Chain B
4541–5037（497 aa）
Chain E
12–1275（1264 aa）
Chain E
1573–2479（907 aa）
Chain E
2734–2939（206 aa）
Chain E
3639–4253（615 aa）
Chain E
4541–5037（497 aa）
Chain G
12–1275（1264 aa）
Chain G
1573–2479（907 aa）
Chain G
2734–2939（206 aa）
Chain G
3639–4253（615 aa）
Chain G
4541–5037（497 aa）
Chain I
12–1275（1264 aa）
Chain I
1573–2479（907 aa）
Chain I
2734–2939（206 aa）
Chain I
3639–4253（615 aa）
Chain I
4541–5037（497 aa）
|
Not recorded | ATP ADENOSINE-5'-TRIPHOSPHATE × 4 CFF CAFFEINE × 4 ZN ZINC ION × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE；Blotted for 3-4 seconds on both sides with Whatman ashless filter paper, blot force 3, wait time 30 seconds
|
Resolution 4.80 Å |
| 5TAU Structure of rabbit RyR1 (Caffeine/ATP/EGTA dataset, class 3) Deposited 2016-09-10 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain B
12–1275（1264 aa）
Chain B
1573–2479（907 aa）
Chain B
2734–2939（206 aa）
Chain B
3639–4253（615 aa）
Chain B
4541–5037（497 aa）
Chain E
12–1275（1264 aa）
Chain E
1573–2479（907 aa）
Chain E
2734–2939（206 aa）
Chain E
3639–4253（615 aa）
Chain E
4541–5037（497 aa）
Chain G
12–1275（1264 aa）
Chain G
1573–2479（907 aa）
Chain G
2734–2939（206 aa）
Chain G
3639–4253（615 aa）
Chain G
4541–5037（497 aa）
Chain I
12–1275（1264 aa）
Chain I
1573–2479（907 aa）
Chain I
2734–2939（206 aa）
Chain I
3639–4253（615 aa）
Chain I
4541–5037（497 aa）
|
Not recorded | ATP ADENOSINE-5'-TRIPHOSPHATE × 4 CFF CAFFEINE × 4 ZN ZINC ION × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE；Blotted for 3-4 seconds on both sides with Whatman ashless filter paper, blot force 3, wait time 30 seconds
|
Resolution 6.20 Å |
| 5TAV Structure of rabbit RyR1 (Caffeine/ATP/EGTA dataset, class 4) Deposited 2016-09-10 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain B
12–1275（1264 aa）
Chain B
1573–2479（907 aa）
Chain B
2734–2939（206 aa）
Chain B
3639–4253（615 aa）
Chain B
4541–5037（497 aa）
Chain E
12–1275（1264 aa）
Chain E
1573–2479（907 aa）
Chain E
2734–2939（206 aa）
Chain E
3639–4253（615 aa）
Chain E
4541–5037（497 aa）
Chain G
12–1275（1264 aa）
Chain G
1573–2479（907 aa）
Chain G
2734–2939（206 aa）
Chain G
3639–4253（615 aa）
Chain G
4541–5037（497 aa）
Chain I
12–1275（1264 aa）
Chain I
1573–2479（907 aa）
Chain I
2734–2939（206 aa）
Chain I
3639–4253（615 aa）
Chain I
4541–5037（497 aa）
|
Not recorded | ATP ADENOSINE-5'-TRIPHOSPHATE × 4 CFF CAFFEINE × 4 ZN ZINC ION × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE；Blotted for 3-4 seconds on both sides with Whatman ashless filter paper, blot force 3, wait time 30 seconds
|
Resolution 4.80 Å |
| 5TAW Structure of rabbit RyR1 (ryanodine dataset, all particles) Deposited 2016-09-10 | Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain B
12–1275（1264 aa）
Chain B
1573–2479（907 aa）
Chain B
2734–2939（206 aa）
Chain B
3639–4253（615 aa）
Chain B
4541–5037（497 aa）
Chain E
12–1275（1264 aa）
Chain E
1573–2479（907 aa）
Chain E
2734–2939（206 aa）
Chain E
3639–4253（615 aa）
Chain E
4541–5037（497 aa）
Chain G
12–1275（1264 aa）
Chain G
1573–2479（907 aa）
Chain G
2734–2939（206 aa）
Chain G
3639–4253（615 aa）
Chain G
4541–5037（497 aa）
Chain I
12–1275（1264 aa）
Chain I
1573–2479（907 aa）
Chain I
2734–2939（206 aa）
Chain I
3639–4253（615 aa）
Chain I
4541–5037（497 aa）
|
Not recorded | ZN ZINC ION × 4 CA CALCIUM ION × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE；Blotted for 3-4 seconds on both sides with Whatman ashless filter paper, blot force 3, wait time 30 seconds
|
Resolution 4.40 Å |
| 5TAX Structure of rabbit RyR1 (ryanodine dataset, class 1) Deposited 2016-09-10 | Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain B
12–1275（1264 aa）
Chain B
1573–2479（907 aa）
Chain B
2734–2939（206 aa）
Chain B
3639–4253（615 aa）
Chain B
4541–5037（497 aa）
Chain E
12–1275（1264 aa）
Chain E
1573–2479（907 aa）
Chain E
2734–2939（206 aa）
Chain E
3639–4253（615 aa）
Chain E
4541–5037（497 aa）
Chain G
12–1275（1264 aa）
Chain G
1573–2479（907 aa）
Chain G
2734–2939（206 aa）
Chain G
3639–4253（615 aa）
Chain G
4541–5037（497 aa）
Chain I
12–1275（1264 aa）
Chain I
1573–2479（907 aa）
Chain I
2734–2939（206 aa）
Chain I
3639–4253（615 aa）
Chain I
4541–5037（497 aa）
|
Not recorded | ZN ZINC ION × 4 CA CALCIUM ION × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE；Blotted for 3-4 seconds on both sides with Whatman ashless filter paper, blot force 3, wait time 30 seconds
|
Resolution 6.60 Å |
| 5TAY Structure of rabbit RyR1 (ryanodine dataset, class 2) Deposited 2016-09-10 | Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain B
12–1275（1264 aa）
Chain B
1573–2479（907 aa）
Chain B
2734–2939（206 aa）
Chain B
3639–4253（615 aa）
Chain B
4541–5037（497 aa）
Chain E
12–1275（1264 aa）
Chain E
1573–2479（907 aa）
Chain E
2734–2939（206 aa）
Chain E
3639–4253（615 aa）
Chain E
4541–5037（497 aa）
Chain G
12–1275（1264 aa）
Chain G
1573–2479（907 aa）
Chain G
2734–2939（206 aa）
Chain G
3639–4253（615 aa）
Chain G
4541–5037（497 aa）
Chain I
12–1275（1264 aa）
Chain I
1573–2479（907 aa）
Chain I
2734–2939（206 aa）
Chain I
3639–4253（615 aa）
Chain I
4541–5037（497 aa）
|
Not recorded | ZN ZINC ION × 4 CA CALCIUM ION × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE；Blotted for 3-4 seconds on both sides with Whatman ashless filter paper, blot force 3, wait time 30 seconds
|
Resolution 4.60 Å |
| 5TAZ Structure of rabbit RyR1 (ryanodine dataset, class 3) Deposited 2016-09-10 | Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain B
12–1275（1264 aa）
Chain B
1573–2479（907 aa）
Chain B
2734–2939（206 aa）
Chain B
3639–4253（615 aa）
Chain B
4541–5037（497 aa）
Chain E
12–1275（1264 aa）
Chain E
1573–2479（907 aa）
Chain E
2734–2939（206 aa）
Chain E
3639–4253（615 aa）
Chain E
4541–5037（497 aa）
Chain G
12–1275（1264 aa）
Chain G
1573–2479（907 aa）
Chain G
2734–2939（206 aa）
Chain G
3639–4253（615 aa）
Chain G
4541–5037（497 aa）
Chain I
12–1275（1264 aa）
Chain I
1573–2479（907 aa）
Chain I
2734–2939（206 aa）
Chain I
3639–4253（615 aa）
Chain I
4541–5037（497 aa）
|
Not recorded | ZN ZINC ION × 4 CA CALCIUM ION × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE；Blotted for 3-4 seconds on both sides with Whatman ashless filter paper, blot force 3, wait time 30 seconds
|
Resolution 4.30 Å |
| 5TB0 Structure of rabbit RyR1 (EGTA-only dataset, all particles) Deposited 2016-09-10 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain B
12–1275（1264 aa）
Chain B
1573–2479（907 aa）
Chain B
2734–2939（206 aa）
Chain B
3639–4253（615 aa）
Chain B
4541–5037（497 aa）
Chain E
12–1275（1264 aa）
Chain E
1573–2479（907 aa）
Chain E
2734–2939（206 aa）
Chain E
3639–4253（615 aa）
Chain E
4541–5037（497 aa）
Chain G
12–1275（1264 aa）
Chain G
1573–2479（907 aa）
Chain G
2734–2939（206 aa）
Chain G
3639–4253（615 aa）
Chain G
4541–5037（497 aa）
Chain I
12–1275（1264 aa）
Chain I
1573–2479（907 aa）
Chain I
2734–2939（206 aa）
Chain I
3639–4253（615 aa）
Chain I
4541–5037（497 aa）
|
Not recorded | ZN ZINC ION × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE；Blotted for 3-4 seconds on both sides with Whatman ashless filter paper, blot force 3, wait time 30 seconds
|
Resolution 4.40 Å |
| 5TB1 Structure of rabbit RyR1 (EGTA-only dataset, class 1) Deposited 2016-09-10 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain B
12–1275（1264 aa）
Chain B
1573–2479（907 aa）
Chain B
2734–2939（206 aa）
Chain B
3639–4253（615 aa）
Chain B
4541–5037（497 aa）
Chain E
12–1275（1264 aa）
Chain E
1573–2479（907 aa）
Chain E
2734–2939（206 aa）
Chain E
3639–4253（615 aa）
Chain E
4541–5037（497 aa）
Chain G
12–1275（1264 aa）
Chain G
1573–2479（907 aa）
Chain G
2734–2939（206 aa）
Chain G
3639–4253（615 aa）
Chain G
4541–5037（497 aa）
Chain I
12–1275（1264 aa）
Chain I
1573–2479（907 aa）
Chain I
2734–2939（206 aa）
Chain I
3639–4253（615 aa）
Chain I
4541–5037（497 aa）
|
Not recorded | ZN ZINC ION × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE；Blotted for 3-4 seconds on both sides with Whatman ashless filter paper, blot force 3, wait time 30 seconds
|
Resolution 4.60 Å |
| 5TB2 Structure of rabbit RyR1 (EGTA-only dataset, class 2) Deposited 2016-09-11 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain B
12–1275（1264 aa）
Chain B
1573–2479（907 aa）
Chain B
2734–2939（206 aa）
Chain B
3639–4253（615 aa）
Chain B
4541–5037（497 aa）
Chain E
12–1275（1264 aa）
Chain E
1573–2479（907 aa）
Chain E
2734–2939（206 aa）
Chain E
3639–4253（615 aa）
Chain E
4541–5037（497 aa）
Chain G
12–1275（1264 aa）
Chain G
1573–2479（907 aa）
Chain G
2734–2939（206 aa）
Chain G
3639–4253（615 aa）
Chain G
4541–5037（497 aa）
Chain I
12–1275（1264 aa）
Chain I
1573–2479（907 aa）
Chain I
2734–2939（206 aa）
Chain I
3639–4253（615 aa）
Chain I
4541–5037（497 aa）
|
Not recorded | ZN ZINC ION × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE；Blotted for 3-4 seconds on both sides with Whatman ashless filter paper, blot force 3, wait time 30 seconds
|
Resolution 4.60 Å |
| 5TB3 Structure of rabbit RyR1 (EGTA-only dataset, class 3) Deposited 2016-09-11 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain B
12–1275（1264 aa）
Chain B
1573–2479（907 aa）
Chain B
2734–2939（206 aa）
Chain B
3639–4253（615 aa）
Chain B
4541–5037（497 aa）
Chain E
12–1275（1264 aa）
Chain E
1573–2479（907 aa）
Chain E
2734–2939（206 aa）
Chain E
3639–4253（615 aa）
Chain E
4541–5037（497 aa）
Chain G
12–1275（1264 aa）
Chain G
1573–2479（907 aa）
Chain G
2734–2939（206 aa）
Chain G
3639–4253（615 aa）
Chain G
4541–5037（497 aa）
Chain I
12–1275（1264 aa）
Chain I
1573–2479（907 aa）
Chain I
2734–2939（206 aa）
Chain I
3639–4253（615 aa）
Chain I
4541–5037（497 aa）
|
Not recorded | ZN ZINC ION × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE；Blotted for 3-4 seconds on both sides with Whatman ashless filter paper, blot force 3, wait time 30 seconds
|
Resolution 4.70 Å |
| 5TB4 Structure of rabbit RyR1 (EGTA-only dataset, class 4) Deposited 2016-09-11 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain B
12–1275（1264 aa）
Chain B
1573–2479（907 aa）
Chain B
2734–2939（206 aa）
Chain B
3639–4253（615 aa）
Chain B
4541–5037（497 aa）
Chain E
12–1275（1264 aa）
Chain E
1573–2479（907 aa）
Chain E
2734–2939（206 aa）
Chain E
3639–4253（615 aa）
Chain E
4541–5037（497 aa）
Chain G
12–1275（1264 aa）
Chain G
1573–2479（907 aa）
Chain G
2734–2939（206 aa）
Chain G
3639–4253（615 aa）
Chain G
4541–5037（497 aa）
Chain I
12–1275（1264 aa）
Chain I
1573–2479（907 aa）
Chain I
2734–2939（206 aa）
Chain I
3639–4253（615 aa）
Chain I
4541–5037（497 aa）
|
Not recorded | ZN ZINC ION × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE；Blotted for 3-4 seconds on both sides with Whatman ashless filter paper, blot force 3, wait time 30 seconds
|
Resolution 4.50 Å |
| 6FG3 Structure of Ryanodine receptor 1 in nanodiscs in the presence of calcium, ATP and ryanodine Deposited 2018-01-09 | Different construct Different mutation/modification Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric |
Chain A
1–1275（1275 aa）
Chain A
1573–2479（907 aa）
Chain A
2734–2939（206 aa）
Chain A
3639–5037（1399 aa）
Chain B
1–1275（1275 aa）
Chain B
1573–2479（907 aa）
Chain B
2734–2939（206 aa）
Chain B
3639–5037（1399 aa）
Chain C
1–1275（1275 aa）
Chain C
1573–2479（907 aa）
Chain C
2734–2939（206 aa）
Chain C
3639–5037（1399 aa）
Chain D
1–1275（1275 aa）
Chain D
1573–2479（907 aa）
Chain D
2734–2939（206 aa）
Chain D
3639–5037（1399 aa）
|
Not recorded | ZN ZINC ION × 4 CA CALCIUM ION × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 7.30 Å |
| 6FOO Structure of Ryanodine Receptor 1 in nanodiscs in the presence of calcium and ATP Deposited 2018-02-08 | Different construct Different mutation/modification Different ligand/ion Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric |
Chain A
1–1297（1297 aa）
Chain A
1573–2479（907 aa）
Chain A
2734–2939（206 aa）
Chain A
3639–5037（1399 aa）
Chain B
1–1297（1297 aa）
Chain B
1573–2479（907 aa）
Chain B
2734–2939（206 aa）
Chain B
3639–5037（1399 aa）
Chain C
1–1297（1297 aa）
Chain C
1573–2479（907 aa）
Chain C
2734–2939（206 aa）
Chain C
3639–5037（1399 aa）
Chain D
1–1297（1297 aa）
Chain D
1573–2479（907 aa）
Chain D
2734–2939（206 aa）
Chain D
3639–5037（1399 aa）
|
Not recorded | ZN ZINC ION × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 8.20 Å |
| 6M2W Structure of RyR1 (Ca2+/Caffeine/ATP/CaM1234/CHL) Deposited 2020-03-01 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric |
Chain A
1–2634（2634 aa）
Chain A
2741–2939（199 aa）
Chain A
3623–4253（631 aa）
Chain A
4541–5037（497 aa）
Chain D
1–2634（2634 aa）
Chain D
2741–2939（199 aa）
Chain D
3623–4253（631 aa）
Chain D
4541–5037（497 aa）
Chain G
1–2634（2634 aa）
Chain G
2741–2939（199 aa）
Chain G
3623–4253（631 aa）
Chain G
4541–5037（497 aa）
Chain J
1–2634（2634 aa）
Chain J
2741–2939（199 aa）
Chain J
3623–4253（631 aa）
Chain J
4541–5037（497 aa）
|
Not recorded | CFF CAFFEINE × 4 CA CALCIUM ION × 4 ATP ADENOSINE-5'-TRIPHOSPHATE × 4 ZN ZINC ION × 4 F0U 5-bromanyl-N-[4-chloranyl-2-methyl-6-(methylcarbamoyl)phenyl]-2-(3-chloranylpyridin-2-yl)pyrazole-3-carboxamide × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.80 Å |
| 6WOT Cryo-EM structure of recombinant rabbit Ryanodine Receptor type 1 mutant R164C in complex with FKBP12.6 Deposited 2020-04-25 | Different mutation/modification Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain A
1–5037（5037 aa）
Chain B
1–5037（5037 aa）
Chain C
1–5037（5037 aa）
Chain D
1–5037（5037 aa）
|
Mutation：R164C Mutation：R164C Mutation：R164C Mutation：R164C | ZN ZINC ION × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.54 Å |
| 7CF9 Structure of RyR1 (Ca2+/CHL) Deposited 2020-06-24 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain A
1–2634（2634 aa）
Chain A
2741–2939（199 aa）
Chain A
3623–4253（631 aa）
Chain A
4541–5037（497 aa）
Chain C
1–2634（2634 aa）
Chain C
2741–2939（199 aa）
Chain C
3623–4253（631 aa）
Chain C
4541–5037（497 aa）
Chain E
1–2634（2634 aa）
Chain E
2741–2939（199 aa）
Chain E
3623–4253（631 aa）
Chain E
4541–5037（497 aa）
Chain G
1–2634（2634 aa）
Chain G
2741–2939（199 aa）
Chain G
3623–4253（631 aa）
Chain G
4541–5037（497 aa）
|
Not recorded | CA CALCIUM ION × 4 ZN ZINC ION × 4 F0U 5-bromanyl-N-[4-chloranyl-2-methyl-6-(methylcarbamoyl)phenyl]-2-(3-chloranylpyridin-2-yl)pyrazole-3-carboxamide × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.70 Å |
| 7M6A High resolution structure of the membrane embedded skeletal muscle ryanodine receptor Deposited 2021-03-25 | Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain A
1–5037（5037 aa）
Chain B
1–5037（5037 aa）
Chain G
1–5037（5037 aa）
Chain I
1–5037（5037 aa）
|
Not recorded | ATP ADENOSINE-5'-TRIPHOSPHATE × 4 CA CALCIUM ION × 4 ZN ZINC ION × 4 CFF CAFFEINE × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.36 Å |
| 7M6L High resolution structure of the membrane embedded skeletal muscle ryanodine receptor Deposited 2021-03-25 | Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain A
1–5037（5037 aa）
Chain B
1–5037（5037 aa）
Chain G
1–5037（5037 aa）
Chain I
1–5037（5037 aa）
|
Not recorded | ATP ADENOSINE-5'-TRIPHOSPHATE × 4 CA CALCIUM ION × 4 ZN ZINC ION × 4 CFF CAFFEINE × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.98 Å |
| 7T64 Rabbit RyR1 disease mutant Y523S in complex with FKBP12.6 embedded in lipidic nanodisc in the closed state Deposited 2021-12-13 | Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain A
1–5037（5037 aa）
Chain B
1–5037（5037 aa）
Chain C
1–5037（5037 aa）
Chain D
1–5037（5037 aa）
|
Mutation：Y523S Mutation：Y523S Mutation：Y523S Mutation：Y523S | ZN ZINC ION × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE；Blot force 2; blot for 1.0s before plunging
|
Resolution 4.00 Å |
| 7T65 Rabbit RyR1 disease mutant Y523S in complex with FKBP12.6 embedded in lipidic nanodisc in the open state Deposited 2021-12-13 | Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain A
1–5037（5037 aa）
Chain B
1–5037（5037 aa）
Chain C
1–5037（5037 aa）
Chain D
1–5037（5037 aa）
|
Mutation：Y523S Mutation：Y523S Mutation：Y523S Mutation：Y523S | ATP ADENOSINE-5'-TRIPHOSPHATE × 4 CA CALCIUM ION × 4 ZN ZINC ION × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE；Blot force 2; blot for 1.0s before plunging
|
Resolution 4.05 Å |
| 7TDG Rabbit RyR1 with AMP-PCP and high Ca2+ embedded in nanodisc in inactivated conformation (Dataset-A) Deposited 2021-12-31 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric |
Chain A
1–1275（1275 aa）
Chain A
1565–2486（922 aa）
Chain A
2704–2941（238 aa）
Chain A
3614–5037（1424 aa）
Chain B
1–1275（1275 aa）
Chain B
1565–2486（922 aa）
Chain B
2704–2941（238 aa）
Chain B
3614–5037（1424 aa）
Chain C
1–1275（1275 aa）
Chain C
1565–2486（922 aa）
Chain C
2704–2941（238 aa）
Chain C
3614–5037（1424 aa）
Chain D
1–1275（1275 aa）
Chain D
1565–2486（922 aa）
Chain D
2704–2941（238 aa）
Chain D
3614–5037（1424 aa）
|
Not recorded | ZN ZINC ION × 4 CA CALCIUM ION × 8 ACP PHOSPHOMETHYLPHOSPHONIC ACID ADENYLATE ESTER × 4 POV (2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate × 8 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE；Sample was blotted for 1 second on both sides with Whatman hardened ashless filter paper with blot force 2.
|
Resolution 3.80 Å |
| 7TDH Rabbit RyR1 with AMP-PCP and high Ca2+ embedded in nanodisc in open conformation Deposited 2021-12-31 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric |
Chain A
1–1275（1275 aa）
Chain A
1565–2486（922 aa）
Chain A
2704–2941（238 aa）
Chain A
3614–5037（1424 aa）
Chain B
1–1275（1275 aa）
Chain B
1565–2486（922 aa）
Chain B
2704–2941（238 aa）
Chain B
3614–5037（1424 aa）
Chain C
1–1275（1275 aa）
Chain C
1565–2486（922 aa）
Chain C
2704–2941（238 aa）
Chain C
3614–5037（1424 aa）
Chain D
1–1275（1275 aa）
Chain D
1565–2486（922 aa）
Chain D
2704–2941（238 aa）
Chain D
3614–5037（1424 aa）
|
Not recorded | ZN ZINC ION × 4 CA CALCIUM ION × 8 ACP PHOSPHOMETHYLPHOSPHONIC ACID ADENYLATE ESTER × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE；Sample was blotted for 1 second on both sides with Whatman hardened ashless filter paper with blot force 2.
|
Resolution 4.00 Å |
| 7TDI Rabbit RyR1 with AMP-PCP and high Ca2+ embedded in nanodisc in closed-inactivated conformation class 2 (Dataset-A) Deposited 2021-12-31 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions | Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric |
Chain A
1–1275（1275 aa）
Chain A
1565–2486（922 aa）
Chain A
2704–2941（238 aa）
Chain A
3614–5037（1424 aa）
Chain B
1–1275（1275 aa）
Chain B
1565–2486（922 aa）
Chain B
2704–2941（238 aa）
Chain B
3614–5037（1424 aa）
Chain C
1–1275（1275 aa）
Chain C
1565–2486（922 aa）
Chain C
2704–2941（238 aa）
Chain C
3614–5037（1424 aa）
Chain D
1–1275（1275 aa）
Chain D
1565–2486（922 aa）
Chain D
2704–2941（238 aa）
Chain D
3614–5037（1424 aa）
|
Not recorded | ACP PHOSPHOMETHYLPHOSPHONIC ACID ADENYLATE ESTER × 4 ZN ZINC ION × 4 CA CALCIUM ION × 8 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE；Sample was blotted for 1 second on both sides with Whatman hardened ashless filter paper with blot force 2.
|
Resolution 3.30 Å |
| 7TDJ Rabbit RyR1 with AMP-PCP and high Ca2+ embedded in nanodisc in closed-inactivated conformation class 1(Dataset-A) Deposited 2021-12-31 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric |
Chain A
1–1275（1275 aa）
Chain A
1565–2486（922 aa）
Chain A
2704–2941（238 aa）
Chain A
3614–5037（1424 aa）
Chain B
1–1275（1275 aa）
Chain B
1565–2486（922 aa）
Chain B
2704–2941（238 aa）
Chain B
3614–5037（1424 aa）
Chain C
1–1275（1275 aa）
Chain C
1565–2486（922 aa）
Chain C
2704–2941（238 aa）
Chain C
3614–5037（1424 aa）
Chain D
1–1275（1275 aa）
Chain D
1565–2486（922 aa）
Chain D
2704–2941（238 aa）
Chain D
3614–5037（1424 aa）
|
Not recorded | ACP PHOSPHOMETHYLPHOSPHONIC ACID ADENYLATE ESTER × 4 ZN ZINC ION × 4 CA CALCIUM ION × 8 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE；Sample was blotted for 1 second on both sides with Whatman hardened ashless filter paper with blot force 2.
|
Resolution 3.70 Å |
| 7TDK Rabbit RyR1 with AMP-PCP and high Ca2+ embedded in nanodisc in closed-inactivated conformation class 3 (Dataset-A) Deposited 2021-12-31 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric |
Chain A
1–1275（1275 aa）
Chain A
1565–2486（922 aa）
Chain A
2704–2941（238 aa）
Chain A
3614–5037（1424 aa）
Chain B
1–1275（1275 aa）
Chain B
1565–2486（922 aa）
Chain B
2704–2941（238 aa）
Chain B
3614–5037（1424 aa）
Chain C
1–1275（1275 aa）
Chain C
1565–2486（922 aa）
Chain C
2704–2941（238 aa）
Chain C
3614–5037（1424 aa）
Chain D
1–1275（1275 aa）
Chain D
1565–2486（922 aa）
Chain D
2704–2941（238 aa）
Chain D
3614–5037（1424 aa）
|
Not recorded | ACP PHOSPHOMETHYLPHOSPHONIC ACID ADENYLATE ESTER × 4 ZN ZINC ION × 4 CA CALCIUM ION × 8 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE；Sample was blotted for 1 second on both sides with Whatman hardened ashless filter paper with blot force 2.
|
Resolution 3.80 Å |
| 7TZC A drug and ATP binding site in type 1 ryanodine receptor Deposited 2022-02-15 | Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric |
Chain A
1–5037（5037 aa）
Chain B
1–5037（5037 aa）
Chain G
1–5037（5037 aa）
Chain I
1–5037（5037 aa）
|
Not recorded | CA CALCIUM ION × 20 ATP ADENOSINE-5'-TRIPHOSPHATE × 8 ZN ZINC ION × 4 CFF CAFFEINE × 4 KVR 4-[(7-methoxy-2,3-dihydro-1,4-benzothiazepin-4(5H)-yl)methyl]benzoic acid × 4 L9R (2S)-3-(octadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate × 8 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.45 Å |
| 8DRP Focus/local refined map in C4 of signal subtracted RyR1 particles Deposited 2022-07-21 | Different ligand/ion Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric |
Chain A
1–5037（5037 aa）
Chain B
1–5037（5037 aa）
Chain C
1–5037（5037 aa）
Chain D
1–5037（5037 aa）
|
Not recorded | CFF CAFFEINE × 4 ATP ADENOSINE-5'-TRIPHOSPHATE × 4 ZN ZINC ION × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.84 Å |
| 8DTB Focus/local refined map in C1 of signal subtracted RyR1 particles in complex with ImperaCalcin Deposited 2022-07-25 | Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 5 PDB declaration: pentameric |
Chain A
1–5037（5037 aa）
Chain D
1–5037（5037 aa）
Chain G
1–5037（5037 aa）
Chain J
1–5037（5037 aa）
|
Not recorded | ATP ADENOSINE-5'-TRIPHOSPHATE × 4 CFF CAFFEINE × 3 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.14 Å |
| 8DUJ Global map in C1 of RyR1 particles in complex with ImperaCalcin Deposited 2022-07-27 | Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 13 PDB declaration: tridecameric |
Chain A
1–5037（5037 aa）
Chain D
1–5037（5037 aa）
Chain G
1–5037（5037 aa）
Chain J
1–5037（5037 aa）
|
Not recorded | CFF CAFFEINE × 4 CA CALCIUM ION × 4 ATP ADENOSINE-5'-TRIPHOSPHATE × 4 ZN ZINC ION × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.70 Å |
| 8DVE RyR1 in presence of IpCa-T26E phosphomimetic and activating ligands Deposited 2022-07-28 | Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric |
Chain A
1–5037（5037 aa）
Chain D
1–5037（5037 aa）
Chain G
1–5037（5037 aa）
Chain J
1–5037（5037 aa）
|
Not recorded | CFF CAFFEINE × 4 CA CALCIUM ION × 4 ATP ADENOSINE-5'-TRIPHOSPHATE × 4 ZN ZINC ION × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.84 Å |
| 8RRT Structure of rabbit RyR1 reconstituted into lipid liposomes in open state in complex with FKBP and Nb9657 Deposited 2024-01-23 | Different construct Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: 12-meric |
Chain B
11–5037（5027 aa）
Chain E
11–5037（5027 aa）
Chain G
11–5037（5027 aa）
Chain J
11–5037（5027 aa）
|
Not recorded | ZN ZINC ION × 4 ATP ADENOSINE-5'-TRIPHOSPHATE × 4 CFF CAFFEINE × 4 CA CALCIUM ION × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.60 Å |
| 8RRU Structure of RyR1 reconstituted into lipid liposomes in primed state in complex with FKBP and Nb9657. Deposited 2024-01-23 | Different construct Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: 12-meric |
Chain B
11–5037（5027 aa）
Chain E
11–5037（5027 aa）
Chain G
11–5037（5027 aa）
Chain J
11–5037（5027 aa）
|
Not recorded | ZN ZINC ION × 4 ATP ADENOSINE-5'-TRIPHOSPHATE × 4 CFF CAFFEINE × 4 CA CALCIUM ION × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.70 Å |
| 8RRV Structure of RyR1 in detergent in close state in complex with FKBP and Nb9657. Deposited 2024-01-23 | Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: 12-meric |
Chain B
1–5037（5037 aa）
Chain E
1–5037（5037 aa）
Chain G
1–5037（5037 aa）
Chain J
1–5037（5037 aa）
|
Not recorded | ZN ZINC ION × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.20 Å |
| 8RRW Structure of RyR1 in detergent in open state in complex with FKBP and Nb9657. Deposited 2024-01-23 | Different construct Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: 12-meric |
Chain B
11–5037（5027 aa）
Chain E
11–5037（5027 aa）
Chain G
11–5037（5027 aa）
Chain J
11–5037（5027 aa）
|
Not recorded | ZN ZINC ION × 4 ATP ADENOSINE-5'-TRIPHOSPHATE × 4 CFF CAFFEINE × 4 CA CALCIUM ION × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.20 Å |
| 8RRX Structure of RyR1 reconstituted into lipid nanodisc in primed state in complex with Ca2+, ATP, caffeine and Nb9657 Deposited 2024-01-23 | Different construct Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: 12-meric |
Chain A
11–5037（5027 aa）
Chain D
11–5037（5027 aa）
Chain G
11–5037（5027 aa）
Chain I
11–5037（5027 aa）
|
Not recorded | ZN ZINC ION × 4 POV (2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate × 4 ATP ADENOSINE-5'-TRIPHOSPHATE × 4 CFF CAFFEINE × 4 CA CALCIUM ION × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.10 Å |
| 8RS0 Structure of RyR1 in detergent in primed state in complex with nanobody and FKBP Deposited 2024-01-24 | Different construct Different oligomeric state Different ligand/ion | Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: 12-meric |
Chain B
11–5037（5027 aa）
Chain E
11–5037（5027 aa）
Chain G
11–5037（5027 aa）
Chain J
11–5037（5027 aa）
|
Not recorded | ZN ZINC ION × 4 ATP ADENOSINE-5'-TRIPHOSPHATE × 4 CFF CAFFEINE × 4 CA CALCIUM ION × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.30 Å |
| 8SEN Cryo-EM Structure of RyR1 Deposited 2023-04-10 | Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Insufficient information Heteromer；Protein × 8 PDB declaration: octameric |
Chain A
1–5037（5037 aa）
Chain B
1–5037（5037 aa）
Chain C
1–5037（5037 aa）
Chain D
1–5037（5037 aa）
|
Not recorded | ZN ZINC ION × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.49 Å |
| 8SEO Cryo-EM Structure of RyR1 + ATP-gamma-S Deposited 2023-04-10 | Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Insufficient information Heteromer；Protein × 8 PDB declaration: octameric |
Chain A
1–5037（5037 aa）
Chain B
1–5037（5037 aa）
Chain C
1–5037（5037 aa）
Chain D
1–5037（5037 aa）
|
Not recorded | AGS PHOSPHOTHIOPHOSPHORIC ACID-ADENYLATE ESTER × 4 ZN ZINC ION × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.92 Å |
| 8SEP Cryo-EM Structure of RyR1 + ADP Deposited 2023-04-10 | Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Insufficient information Heteromer；Protein × 8 PDB declaration: octameric |
Chain A
1–5037（5037 aa）
Chain B
1–5037（5037 aa）
Chain C
1–5037（5037 aa）
Chain D
1–5037（5037 aa）
|
Not recorded | ADP ADENOSINE-5'-DIPHOSPHATE × 4 ZN ZINC ION × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.57 Å |
| 8SEQ Cryo-EM Structure of RyR1 + AMP Deposited 2023-04-10 | Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Insufficient information Heteromer；Protein × 8 PDB declaration: octameric |
Chain A
1–5037（5037 aa）
Chain B
1–5037（5037 aa）
Chain C
1–5037（5037 aa）
Chain D
1–5037（5037 aa）
|
Not recorded | AMP ADENOSINE MONOPHOSPHATE × 4 ZN ZINC ION × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.40 Å |
| 8SER Cryo-EM Structure of RyR1 + Adenosine Deposited 2023-04-10 | Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Insufficient information Heteromer；Protein × 8 PDB declaration: octameric |
Chain A
1–5037（5037 aa）
Chain B
1–5037（5037 aa）
Chain C
1–5037（5037 aa）
Chain D
1–5037（5037 aa）
|
Not recorded | ADN ADENOSINE × 4 ZN ZINC ION × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.42 Å |
| 8SES Cryo-EM Structure of RyR1 + Adenine Deposited 2023-04-10 | Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Insufficient information Heteromer；Protein × 8 PDB declaration: octameric |
Chain A
1–5037（5037 aa）
Chain B
1–5037（5037 aa）
Chain C
1–5037（5037 aa）
Chain D
1–5037（5037 aa）
|
Not recorded | ZN ZINC ION × 4 ADE ADENINE × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.98 Å |
| 8SET Cryo-EM Structure of RyR1 + cAMP Deposited 2023-04-10 | Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Insufficient information Heteromer；Protein × 8 PDB declaration: octameric |
Chain A
1–5037（5037 aa）
Chain B
1–5037（5037 aa）
Chain C
1–5037（5037 aa）
Chain D
1–5037（5037 aa）
|
Not recorded | CMP ADENOSINE-3',5'-CYCLIC-MONOPHOSPHATE × 4 ZN ZINC ION × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.42 Å |
| 8SEU Cryo-EM Structure of RyR1 (Local Refinement of TMD) Deposited 2023-04-10 | Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric |
Chain A
1–5037（5037 aa）
Chain B
1–5037（5037 aa）
Chain C
1–5037（5037 aa）
Chain D
1–5037（5037 aa）
|
Not recorded | ZN ZINC ION × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.00 Å |
| 8SEV Cryo-EM Structure of RyR1 + ATP-gamma-S (Local Refinement of TMD) Deposited 2023-04-10 | Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric |
Chain A
1–5037（5037 aa）
Chain B
1–5037（5037 aa）
Chain C
1–5037（5037 aa）
Chain D
1–5037（5037 aa）
|
Not recorded | AGS PHOSPHOTHIOPHOSPHORIC ACID-ADENYLATE ESTER × 4 ZN ZINC ION × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.17 Å |
| 8SEW Cryo-EM Structure of RyR1 + ADP (Local Refinement of TMD) Deposited 2023-04-10 | Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric |
Chain A
1–5037（5037 aa）
Chain B
1–5037（5037 aa）
Chain C
1–5037（5037 aa）
Chain D
1–5037（5037 aa）
|
Not recorded | ADP ADENOSINE-5'-DIPHOSPHATE × 4 ZN ZINC ION × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.89 Å |
| 8SEX Cryo-EM Structure of RyR1 + AMP (Local Refinement of TMD) Deposited 2023-04-10 | Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric |
Chain A
1–5037（5037 aa）
Chain B
1–5037（5037 aa）
Chain C
1–5037（5037 aa）
Chain D
1–5037（5037 aa）
|
Not recorded | AMP ADENOSINE MONOPHOSPHATE × 4 ZN ZINC ION × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.84 Å |
| 8SEY Cryo-EM Structure of RyR1 + Adenosine (Local Refinement of TMD) Deposited 2023-04-10 | Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric |
Chain A
1–5037（5037 aa）
Chain B
1–5037（5037 aa）
Chain C
1–5037（5037 aa）
Chain D
1–5037（5037 aa）
|
Not recorded | ADN ADENOSINE × 4 ZN ZINC ION × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.99 Å |
| 8SEZ Cryo-EM Structure of RyR1 + Adenine (Local Refinement of TMD) Deposited 2023-04-10 | Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric |
Chain A
1–5037（5037 aa）
Chain B
1–5037（5037 aa）
Chain C
1–5037（5037 aa）
Chain D
1–5037（5037 aa）
|
Not recorded | ADE ADENINE × 4 ZN ZINC ION × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.54 Å |
| 8SF0 Cryo-EM Structure of RyR1 + cAMP (Local Refinement of TMD) Deposited 2023-04-10 | Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric |
Chain A
1–5037（5037 aa）
Chain B
1–5037（5037 aa）
Chain C
1–5037（5037 aa）
Chain D
1–5037（5037 aa）
|
Not recorded | CMP ADENOSINE-3',5'-CYCLIC-MONOPHOSPHATE × 4 ZN ZINC ION × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.90 Å |
| 8X48 Cryo-EM structure of Ryanodine receptor 1 (EGTA) Deposited 2023-11-15 | Different ligand/ion Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric |
Chain A
1–5037（5037 aa）
Chain B
1–5037（5037 aa）
Chain C
1–5037（5037 aa）
Chain D
1–5037（5037 aa）
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.00 Å |
| 8X49 Cryo-EM structure of Ryanodine receptor 1 (100 nM Ca2+) Deposited 2023-11-15 | Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric |
Chain A
1–5037（5037 aa）
Chain B
1–5037（5037 aa）
Chain C
1–5037（5037 aa）
Chain D
1–5037（5037 aa）
|
Not recorded | CA CALCIUM ION × 4 ZN ZINC ION × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.40 Å |
| 8X4A Cryo-EM structure of Ryanodine receptor 1 (TM helix S0,100 nM Ca2+, closed state) Deposited 2023-11-15 | Different ligand/ion Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric |
Chain A
1–5037（5037 aa）
Chain B
1–5037（5037 aa）
Chain C
1–5037（5037 aa）
Chain D
1–5037（5037 aa）
|
Not recorded | CA CALCIUM ION × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.80 Å |
| 8X4B Cryo-EM structure of Ryanodine receptor 1 (TM helix S0,100 nM Ca2+, open state) Deposited 2023-11-15 | Different ligand/ion Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric |
Chain A
1–5037（5037 aa）
Chain B
1–5037（5037 aa）
Chain C
1–5037（5037 aa）
Chain D
1–5037（5037 aa）
|
Not recorded | CA CALCIUM ION × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.20 Å |
| 8X4C Cryo-EM structure of Ryanodine receptor 1 (20 uM Ca2+, 2 mM ATP) Deposited 2023-11-15 | Different ligand/ion Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric |
Chain A
1–5037（5037 aa）
Chain B
1–5037（5037 aa）
Chain C
1–5037（5037 aa）
Chain D
1–5037（5037 aa）
|
Not recorded | ATP ADENOSINE-5'-TRIPHOSPHATE × 4 CA CALCIUM ION × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.80 Å |
| 8X4D Cryo-EM structure of Ryanodine receptor 1 (TM helix S0,20 uM Ca2+, 2 mM ATP) Deposited 2023-11-15 | Different ligand/ion Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric |
Chain A
1–5037（5037 aa）
Chain B
1–5037（5037 aa）
Chain C
1–5037（5037 aa）
Chain D
1–5037（5037 aa）
|
Not recorded | CA CALCIUM ION × 4 ATP ADENOSINE-5'-TRIPHOSPHATE × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.90 Å |
| 8XJI Structure of chimeric RyR complex with flubendiamide Deposited 2023-12-21 | Different mutation/modification Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric |
Chain A
1–5037（5037 aa）
Chain B
1–5037（5037 aa）
Chain C
1–5037（5037 aa）
Chain D
1–5037（5037 aa）
|
Mutation：R4563K, F4564Y,C4657I, L4792S Mutation：R4563K, F4564Y,C4657I, L4792S Mutation：R4563K, F4564Y,C4657I, L4792S Mutation：R4563K, F4564Y,C4657I, L4792S | A1LVX Flubendiamide × 4 ZN ZINC ION × 4 CA CALCIUM ION × 4 ATP ADENOSINE-5'-TRIPHOSPHATE × 4 CFF CAFFEINE × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.91 Å |
| 8XKH Structure of chimeric RyR Complex with tetraniliprole Deposited 2023-12-23 | Different mutation/modification Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric |
Chain A
1–5037（5037 aa）
Chain B
1–5037（5037 aa）
Chain C
1–5037（5037 aa）
Chain D
1–5037（5037 aa）
|
Mutation：R4563K, F4564Y,C4657I, L4792S Mutation：R4563K, F4564Y,C4657I, L4792S Mutation：R4563K, F4564Y,C4657I, L4792S Mutation：R4563K, F4564Y,C4657I, L4792S | ZN ZINC ION × 4 CA CALCIUM ION × 4 ATP ADENOSINE-5'-TRIPHOSPHATE × 4 CFF CAFFEINE × 4 A1LV1 tetraniliprole × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.87 Å |
| 8XLF Structure of chimeric RyR Deposited 2023-12-25 | Different mutation/modification Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric |
Chain A
1–5037（5037 aa）
Chain B
1–5037（5037 aa）
Chain C
1–5037（5037 aa）
Chain D
1–5037（5037 aa）
|
Mutation：R4563K, F4564Y,C4657I, L4792S Mutation：R4563K, F4564Y,C4657I, L4792S Mutation：R4563K, F4564Y,C4657I, L4792S Mutation：R4563K, F4564Y,C4657I, L4792S | CA CALCIUM ION × 4 ZN ZINC ION × 4 ATP ADENOSINE-5'-TRIPHOSPHATE × 4 CFF CAFFEINE × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.62 Å |
| 8XLH Structure of chimeric RyR-I4657M/G4819E Deposited 2023-12-26 | Different mutation/modification Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric |
Chain A
1–5037（5037 aa）
Chain B
1–5037（5037 aa）
Chain C
1–5037（5037 aa）
Chain D
1–5037（5037 aa）
|
Mutation：R4563K, F4564Y,C4657M, L4792S,G4819E Mutation：R4563K, F4564Y,C4657M, L4792S,G4819E Mutation：R4563K, F4564Y,C4657M, L4792S,G4819E Mutation：R4563K, F4564Y,C4657M, L4792S,G4819E | ZN ZINC ION × 4 CA CALCIUM ION × 4 ATP ADENOSINE-5'-TRIPHOSPHATE × 4 CFF CAFFEINE × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.62 Å |
| 8Y40 Structure of chimeric RyR-I4657M/G4819E complex with chlorantraniliprole Deposited 2024-01-29 | Different mutation/modification Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: 12-meric |
Chain A
1–5037（5037 aa）
Chain B
1–5037（5037 aa）
Chain C
1–5037（5037 aa）
Chain D
1–5037（5037 aa）
|
Mutation：R4563K, F4564Y,C4657M, L4792S,G4819E Mutation：R4563K, F4564Y,C4657M, L4792S,G4819E Mutation：R4563K, F4564Y,C4657M, L4792S,G4819E Mutation：R4563K, F4564Y,C4657M, L4792S,G4819E | F0U 5-bromanyl-N-[4-chloranyl-2-methyl-6-(methylcarbamoyl)phenyl]-2-(3-chloranylpyridin-2-yl)pyrazole-3-carboxamide × 4 ZN ZINC ION × 4 CA CALCIUM ION × 4 ATP ADENOSINE-5'-TRIPHOSPHATE × 4 CFF CAFFEINE × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.58 Å |
| 9CGP RyR1 disease mutant Y523S with FKBP12.6, nanodisc and inhibitor dantrolene in the absence of calcium with refined P1 domain Deposited 2024-06-30 | Different mutation/modification Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain A
1–5037（5037 aa）
Chain B
1–5037（5037 aa）
Chain C
1–5037（5037 aa）
Chain D
1–5037（5037 aa）
|
Mutation：Y523S Mutation：Y523S Mutation：Y523S Mutation：Y523S | ZN ZINC ION × 4 ATP ADENOSINE-5'-TRIPHOSPHATE × 4 U1C Dantrolene × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.34 Å |
| 9CGQ Rabbit RyR1 disease mutant Y523S in complex with FKBP12.6, nanodisc and inhibitor dantrolene in the presence of activating calcium Deposited 2024-06-30 | Different mutation/modification Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain A
1–5037（5037 aa）
Chain B
1–5037（5037 aa）
Chain C
1–5037（5037 aa）
Chain D
1–5037（5037 aa）
|
Mutation：Y523S Mutation：Y523S Mutation：Y523S Mutation：Y523S | ZN ZINC ION × 4 ATP ADENOSINE-5'-TRIPHOSPHATE × 4 CA CALCIUM ION × 4 U1C Dantrolene × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.23 Å |
| 9E17 Structure of RyR1 in the primed state in the presence of caffeine (reprocessed/reanalyzed from EMPIAR-10997, 7TZC, EMD-26205) Deposited 2024-10-21 | Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: 12-meric |
Chain A
1–5037（5037 aa）
Chain B
1–5037（5037 aa）
Chain G
1–5037（5037 aa）
Chain I
1–5037（5037 aa）
|
Not recorded | CA CALCIUM ION × 20 ATP ADENOSINE-5'-TRIPHOSPHATE × 8 ZN ZINC ION × 4 CFF CAFFEINE × 4 KVR 4-[(7-methoxy-2,3-dihydro-1,4-benzothiazepin-4(5H)-yl)methyl]benzoic acid × 4 L9R (2S)-3-(octadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate × 8 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.45 Å |
| 9E18 Structure of RyR1 in the primed state in the presence of pentoxifylline Deposited 2024-10-21 | Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain A
1–5037（5037 aa）
Chain B
1–5037（5037 aa）
Chain C
1–5037（5037 aa）
Chain D
1–5037（5037 aa）
|
Not recorded | ATP ADENOSINE-5'-TRIPHOSPHATE × 4 CA CALCIUM ION × 4 ZN ZINC ION × 4 PNX 3,7-DIMETHYL-1-(5-OXOHEXYL)-3,7-DIHYDRO-1H-PURINE-2,6-DIONE × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4；2 mM pentoxifylline was added to the final sample from a 20 mM stock solution in buffer.
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.68 Å |
| 9E19 Structure of RyR1 in the open state in the presence of pentoxifylline Deposited 2024-10-21 | Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain A
1–5037（5037 aa）
Chain B
1–5037（5037 aa）
Chain C
1–5037（5037 aa）
Chain D
1–5037（5037 aa）
|
Not recorded | ATP ADENOSINE-5'-TRIPHOSPHATE × 4 CA CALCIUM ION × 4 ZN ZINC ION × 4 PNX 3,7-DIMETHYL-1-(5-OXOHEXYL)-3,7-DIHYDRO-1H-PURINE-2,6-DIONE × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4；2 mM pentoxifylline was added to the final sample from a 20 mM stock solution in buffer.
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.04 Å |
| 9E1A Structure of RyR1 in the primed state in the presence of dyphylline Deposited 2024-10-21 | Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain A
1–5037（5037 aa）
Chain B
1–5037（5037 aa）
Chain C
1–5037（5037 aa）
Chain D
1–5037（5037 aa）
|
Not recorded | ATP ADENOSINE-5'-TRIPHOSPHATE × 4 CA CALCIUM ION × 4 ZN ZINC ION × 4 A1BD2 dyphylline × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4；2 mM dyphylline was added to the final sample from a 20 mM stock solution in buffer.
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.35 Å |
| 9E1B Structure of RyR1 in the open state in the presence of dyphylline Deposited 2024-10-21 | Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain A
1–5037（5037 aa）
Chain B
1–5037（5037 aa）
Chain C
1–5037（5037 aa）
Chain D
1–5037（5037 aa）
|
Not recorded | ATP ADENOSINE-5'-TRIPHOSPHATE × 4 CA CALCIUM ION × 4 ZN ZINC ION × 4 A1BD2 dyphylline × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4；2 mM dyphylline was added to the final sample from a 20 mM stock solution in buffer.
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.49 Å |
| 9E1C Structure of RyR1 in the primed state in the presence of IBMX Deposited 2024-10-21 | Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain A
1–5037（5037 aa）
Chain B
1–5037（5037 aa）
Chain C
1–5037（5037 aa）
Chain D
1–5037（5037 aa）
|
Not recorded | ATP ADENOSINE-5'-TRIPHOSPHATE × 4 CA CALCIUM ION × 4 ZN ZINC ION × 4 IBM 3-ISOBUTYL-1-METHYLXANTHINE × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4；2 mM IBMX was added to the final sample from a 100 mM stock solution in 100% DMSO.
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.63 Å |
| 9E1D Structure of RyR1 in the primed state in the presence of enprofylline Deposited 2024-10-21 | Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain A
1–5037（5037 aa）
Chain B
1–5037（5037 aa）
Chain C
1–5037（5037 aa）
Chain D
1–5037（5037 aa）
|
Not recorded | ATP ADENOSINE-5'-TRIPHOSPHATE × 4 CA CALCIUM ION × 4 ZN ZINC ION × 4 A1BD3 enprofylline × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4；2 mM enprofylline was added to the final sample from a 100 mM stock solution in 100% DMSO.
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.76 Å |
| 9E1E Structure of RyR1 in the primed state in the presence of uracil Deposited 2024-10-21 | Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain A
1–5037（5037 aa）
Chain B
1–5037（5037 aa）
Chain C
1–5037（5037 aa）
Chain D
1–5037（5037 aa）
|
Not recorded | ATP ADENOSINE-5'-TRIPHOSPHATE × 4 CA CALCIUM ION × 4 ZN ZINC ION × 4 URA URACIL × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4；2 mM uracil was added to the final sample from a 100 mM stock solution in 100% DMSO.
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.92 Å |
| 9E1F Structure of RyR1 in the primed state in the presence of allopurinol Deposited 2024-10-21 | Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain A
1–5037（5037 aa）
Chain B
1–5037（5037 aa）
Chain C
1–5037（5037 aa）
Chain D
1–5037（5037 aa）
|
Not recorded | ATP ADENOSINE-5'-TRIPHOSPHATE × 4 CA CALCIUM ION × 4 ZN ZINC ION × 4 A1BD4 allopurinol × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4；2 mM allopurinol was added to the final sample from a 100 mM stock solution in 100% DMSO.
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.03 Å |
| 9E1G Structure of RyR1 in the primed state in the presence of oxypurinol Deposited 2024-10-21 | Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain A
1–5037（5037 aa）
Chain B
1–5037（5037 aa）
Chain C
1–5037（5037 aa）
Chain D
1–5037（5037 aa）
|
Not recorded | ATP ADENOSINE-5'-TRIPHOSPHATE × 4 CA CALCIUM ION × 4 ZN ZINC ION × 4 141 Oxypurinol × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4；2 mM oxypurinol was added to the final sample from a 100 mM stock solution in 100% DMSO.
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.17 Å |
| 9E1H Structure of RyR1 in the primed state in the presence of oxopyricid Deposited 2024-10-21 | Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain A
1–5037（5037 aa）
Chain B
1–5037（5037 aa）
Chain C
1–5037（5037 aa）
Chain D
1–5037（5037 aa）
|
Not recorded | ATP ADENOSINE-5'-TRIPHOSPHATE × 4 CA CALCIUM ION × 4 ZN ZINC ION × 4 A1BD5 oxopyricid × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4；2 mM oxopyricid was added to the final sample from a 20 mM stock solution in buffer.
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.26 Å |
| 9E1I Structure of RyR1 in the open state in the presence of oxopyricid Deposited 2024-10-21 | Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain A
1–5037（5037 aa）
Chain B
1–5037（5037 aa）
Chain C
1–5037（5037 aa）
Chain D
1–5037（5037 aa）
|
Not recorded | ATP ADENOSINE-5'-TRIPHOSPHATE × 4 CA CALCIUM ION × 4 ZN ZINC ION × 4 A1BD5 oxopyricid × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4；2 mM oxopyricid was added to the final sample from a 20 mM stock solution in buffer.
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.20 Å |
| 9HEO Open-state RyR1 in 0.05% POPC micelles, in complex with a nanobody and FKBP Deposited 2024-11-14 | Different construct Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: 12-meric |
Chain A
11–5037（5027 aa）
Chain C
11–5037（5027 aa）
Chain G
11–5037（5027 aa）
Chain J
11–5037（5027 aa）
|
Not recorded | ZN ZINC ION × 4 ATP ADENOSINE-5'-TRIPHOSPHATE × 4 CFF CAFFEINE × 4 CA CALCIUM ION × 4 POV (2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate × 72 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.40 Å |
| 9HEQ Open-state RyR1 in 0.01% POPC micelles, in complex with a nanobody and FKBP12 Deposited 2024-11-14 | Different construct Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: 12-meric |
Chain A
11–5037（5027 aa）
Chain C
11–5037（5027 aa）
Chain G
11–5037（5027 aa）
Chain J
11–5037（5027 aa）
|
Not recorded | POV (2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate × 40 ATP ADENOSINE-5'-TRIPHOSPHATE × 4 CFF CAFFEINE × 4 CA CALCIUM ION × 4 ZN ZINC ION × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.60 Å |
| 9OL4 Cryo-EM Structure of Ryanodine Receptor 1: Drug Bound Open Conformation Deposited 2025-05-12 | Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain A
1–5037（5037 aa）
Chain G
1–5037（5037 aa）
Chain M
1–5037（5037 aa）
Chain S
1–5037（5037 aa）
|
Not recorded | CFF CAFFEINE × 4 CA CALCIUM ION × 4 117 7-[2-(4-FLUORO-PHENYL)-5-ISOPROPYL-3-PHENYL-4-PHENYLCARBAMOYL-PYRROL-1-YL]- 3,5-DIHYDROXY-HEPTANOIC ACID × 12 ZN ZINC ION × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.37 Å |
| 9OL5 Rabbit Ryanodine Receptor 1: Atorvastatin Bound Closed Conformation Deposited 2025-05-12 | Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain A
1–5037（5037 aa）
Chain G
1–5037（5037 aa）
Chain M
1–5037（5037 aa）
Chain S
1–5037（5037 aa）
|
Not recorded | CA CALCIUM ION × 4 117 7-[2-(4-FLUORO-PHENYL)-5-ISOPROPYL-3-PHENYL-4-PHENYLCARBAMOYL-PYRROL-1-YL]- 3,5-DIHYDROXY-HEPTANOIC ACID × 4 ZN ZINC ION × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.56 Å |
| 9OL6 Rabbit Ryanodine Receptor 1: DMSO Control Closed Conformation Deposited 2025-05-12 | Different oligomeric state Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain A
1–5037（5037 aa）
Chain G
1–5037（5037 aa）
Chain M
1–5037（5037 aa）
Chain S
1–5037（5037 aa）
|
Not recorded | CA CALCIUM ION × 4 ZN ZINC ION × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.11 Å |
| 9R8O Primed-state RyR1 in 0.05% POPC micelles, in complex with a nanobody and FKBP Deposited 2025-05-16 | Different construct Different oligomeric state Different ligand/ion | Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: 12-meric |
Chain C
11–5037（5027 aa）
Chain F
11–5037（5027 aa）
Chain I
11–5037（5027 aa）
Chain J
11–5037（5027 aa）
|
Not recorded | ATP ADENOSINE-5'-TRIPHOSPHATE × 4 CFF CAFFEINE × 4 CA CALCIUM ION × 4 POV (2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate × 40 ZN ZINC ION × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.30 Å |
| 9RCW Primed-state RyR1 in 0.01% POPC micelles, in complex with a nanobody and FKBP12 Deposited 2025-05-30 | Different construct Different oligomeric state Different ligand/ion | Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: 12-meric |
Chain A
11–5037（5027 aa）
Chain C
11–5037（5027 aa）
Chain G
11–5037（5027 aa）
Chain J
11–5037（5027 aa）
|
Not recorded | ZN ZINC ION × 4 ATP ADENOSINE-5'-TRIPHOSPHATE × 4 CFF CAFFEINE × 4 CA CALCIUM ION × 4 POV (2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate × 24 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.30 Å |
124 other PDB entries and 135 assemblies. Open the comparison page and filter oligomeric states
View Construct and Data Evidence
| UniProt name | RYR1_RABIT |
| Isoform | — |
| PDB entities | 1 |
| Chains and sequence ranges | Author chain A； PDBConstruct 1–5037； UniProt 1–5037 Author chain B； PDBConstruct 1–5037； UniProt 1–5037 Author chain C； PDBConstruct 1–5037； UniProt 1–5037 Author chain D； PDBConstruct 1–5037； UniProt 1–5037 |
