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5aj3

Structure of the small subunit of the mammalian mitoribosome

Method: ELECTRON MICROSCOPY Dmax: 214.6 Å Quality: GOOD
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1. Protein Identity and Related Structures Protein Identity & Related Structures

MITORIBOSOMAL PROTEIN US2M, MRPS2

OrganismNot specified

UniProt F1S001

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein–RNA Heteromer Protein × 33 RNA 4 PDB declaration: 37-meric（37） Consistent with all polymer counts Chain B； UniProt 1–289 Not recorded MITORIBOSOMAL 12S RRNA × 1 MITORIBOSOMAL PROTEIN US3M, MRPS24 × 1 （F1ST79） MITORIBOSOMAL PROTEIN US5M, MRPS5 × 1 （F1SU66） MITORIBOSOMAL PROTEIN BS6M, MRPS6 × 1 MITORIBOSOMAL PROTEIN US7M, MRPS7 × 1 （F1RVX4） MITORIBOSOMAL PROTEIN US9M, MRPS9 × 1 MITORIBOSOMAL PROTEIN US10M, MRPS10 × 1 MITORIBOSOMAL PROTEIN US11M, MRPS11 × 1 （F1SRT1） MITORIBOSOMAL PROTEIN US12M, MRPS12 × 1 MITORIBOSOMAL PROTEIN US14M, MRPS14 × 1 MITORIBOSOMAL PROTEIN US15M, MRPS15 × 1 MITORIBOSOMAL PROTEIN BS16M, MRPS16 × 1 （F1SU70） MITORIBOSOMAL PROTEIN US17M, MRPS17 × 1 （F1RIU0） MITORIBOSOMAL PROTEIN BS18M, MRPS18C × 1 MITORIBOSOMAL PROTEIN BL19M, MRPL19 × 1 MITORIBOSOMAL PROTEIN BS21M, MRPS21 × 1 （I3L971） P-SITE AND A-SITE TRNA × 2 MRNA × 1 MITORIBOSOMAL PROTEIN MS22, MRPS22 × 1 MITORIBOSOMAL PROTEIN MS23, MRPS23 × 1 MITORIBOSOMAL PROTEIN MS25, MRPS25 × 1 MITORIBOSOMAL PROTEIN MS26, MRPS26 × 1 （F1S8A5） MITORIBOSOMAL PROTEIN MS27, MRPS27 × 1 MITORIBOSOMAL PROTEIN MS28, MRPS28 × 1 MITORIBOSOMAL PROTEIN MS29, MRPS29 × 1 MITORIBOSOMAL PROTEIN MS31, MRPS31 × 1 （F1RME2） MITORIBOSOMAL PROTEIN MS33, MRPS33 × 1 （F1S8G3） MITORIBOSOMAL PROTEIN MS34, MRPS34 × 1 MITORIBOSOMAL PROTEIN MS35, MRPS35 × 1 （F1SG95） MITORIBOSOMAL PROTEIN MS37, MRPS37 × 1 （F1SU49） MITORIBOSOMAL PROTEIN MS38, MRPS38 × 1 MITORIBOSOMAL PROTEIN MS39, MRPS39 × 1 （K7GKS8） MITORIBOSOMAL PROTEIN MS40, MRPS18B × 1 （Q767K8） UNASSIGNED HELICES × 1 UNASSIGNED HELICES × 1 MG MAGNESIUM ION × 144 ZN ZINC ION × 3 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY cryo-EM buffer：20 MM HEPES-KOH, 50 MM KCL, 40 MM MGCL2, 1 MM DTT；pH 7.4；20 MM HEPES-KOH, 50 MM KCL, 40 MM MGCL2, 1 MM DTT cryo-EM vitrification conditions：Cryogen ETHANE-PROPANE；MIXTURE OF LIQUID ETHANE AND PROPANE Resolution 3.60 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 5AJ3 Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
5AJ4 Structure of the 55S mammalian mitoribosome. Deposited 2015-02-20 Different construct Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 83 PDB declaration: 89-meric
Chain AB 55–274（220 aa）
Not recorded MG MAGNESIUM ION × 351 ZN ZINC ION × 6 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer 20 MM HEPES-KOH, 50 MM KCL, 40 MM MGCL2, 1 MM DTT；pH 7.4；20 MM HEPES-KOH, 50 MM KCL, 40 MM MGCL2, 1 MM DTT
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；MIXTURE OF LIQUID ETHANE AND PROPANE
Resolution 3.80 Å
6GAW Unique features of mammalian mitochondrial translation initiation revealed by cryo-EM. This file contains the complete 55S ribosome. Deposited 2018-04-13 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 90 PDB declaration: 95-meric
Chain AB 1–289（289 aa）
Not recorded MG MAGNESIUM ION × 326 ZN ZINC ION × 6 5GP GUANOSINE-5'-MONOPHOSPHATE × 2 SPM SPERMINE × 3 GSP 5'-GUANOSINE-DIPHOSPHATE-MONOTHIOPHOSPHATE × 1 NA SODIUM ION × 1 FME N-FORMYLMETHIONINE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
6GAZ Unique features of mammalian mitochondrial translation initiation revealed by cryo-EM. This file contains the 28S ribosomal subunit. Deposited 2018-04-13 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 33 PDB declaration: 36-meric
Chain AB 1–289（289 aa）
Not recorded GSP 5'-GUANOSINE-DIPHOSPHATE-MONOTHIOPHOSPHATE × 1 MG MAGNESIUM ION × 111 NA SODIUM ION × 1 SPM SPERMINE × 1 ZN ZINC ION × 3 FME N-FORMYLMETHIONINE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
6YDP 55S mammalian mitochondrial ribosome with mtEFG1 and P site fMet-tRNAMet (POST) Deposited 2020-03-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 89 PDB declaration: 94-meric
Chain AB 1–289（289 aa）
Not recorded MG MAGNESIUM ION × 326 ZN ZINC ION × 6 SPM SPERMINE × 3 FME N-FORMYLMETHIONINE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 5GP GUANOSINE-5'-MONOPHOSPHATE × 2 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.00 Å
6YDW 55S mammalian mitochondrial ribosome with mtEFG1 and two tRNAMet (TI-POST) Deposited 2020-03-21 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 89 PDB declaration: 95-meric
Chain AB 1–289（289 aa）
Not recorded MG MAGNESIUM ION × 325 ZN ZINC ION × 6 SPM SPERMINE × 3 FME N-FORMYLMETHIONINE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 5GP GUANOSINE-5'-MONOPHOSPHATE × 1 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 4.20 Å
7NQH 55S mammalian mitochondrial ribosome with mtRF1a and P-site tRNAMet Deposited 2021-03-01 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 88 PDB declaration: 93-meric
Chain AB 1–289（289 aa）
Not recorded MG MAGNESIUM ION × 323 ZN ZINC ION × 6 SPM SPERMINE × 3 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 5GP GUANOSINE-5'-MONOPHOSPHATE × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.50 Å
7NQL 55S mammalian mitochondrial ribosome with ICT1 and P site tRNAMet Deposited 2021-03-01 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 88 PDB declaration: 93-meric
Chain AB 1–289（289 aa）
Not recorded ZN ZINC ION × 6 MG MAGNESIUM ION × 335 5GP GUANOSINE-5'-MONOPHOSPHATE × 2 SPM SPERMINE × 3 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.40 Å
7NSI 55S mammalian mitochondrial ribosome with mtRRF (pre) and tRNA(P/E) Deposited 2021-03-07 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 88 PDB declaration: 93-meric
Chain AB 1–289（289 aa）
Not recorded MG MAGNESIUM ION × 327 ZN ZINC ION × 6 SPM SPERMINE × 3 5GP GUANOSINE-5'-MONOPHOSPHATE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 4.60 Å
7NSJ 55S mammalian mitochondrial ribosome with tRNA(P/P) and tRNA(E*) Deposited 2021-03-07 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 87 PDB declaration: 93-meric
Chain AB 1–289（289 aa）
Not recorded MG MAGNESIUM ION × 324 ZN ZINC ION × 6 SPM SPERMINE × 3 5GP GUANOSINE-5'-MONOPHOSPHATE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.90 Å
8OIN 55S mammalian mitochondrial ribosome with mtRF1 and P-site tRNA Deposited 2023-03-23 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 88 PDB declaration: 94-meric
Chain Ab 1–289（289 aa）
Not recorded MG MAGNESIUM ION × 332 K POTASSIUM ION × 41 PHE PHENYLALANINE × 1 ZN ZINC ION × 3 FES FE2/S2 (INORGANIC) CLUSTER × 3 SPM SPERMINE × 1 SPD SPERMIDINE × 1 FME N-FORMYLMETHIONINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20mM HEPES-KOH pH=7.5, 100mM KCl, 20mM MgCl2, ~1% sucrose (from mtRF1 storage buffer)
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.60 Å
8OIP 28S mammalian mitochondrial small ribosomal subunit with mtRF1 and P-site tRNA Deposited 2023-03-23 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 33 PDB declaration: 36-meric
Chain Ab 1–289（289 aa）
Not recorded MG MAGNESIUM ION × 127 SPM SPERMINE × 1 SPD SPERMIDINE × 1 K POTASSIUM ION × 11 ZN ZINC ION × 1 FS2 FE-S-O HYBRID CLUSTER × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20mM HEPES-KOH pH=7.5, 100mM KCl, 20mM MgCl2, ~1% sucrose (from mtRF1 storage buffer)
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.60 Å

11 other PDB entries and 11 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name F1S001_PIG
Isoform —
PDB entities 2
Chains and sequence ranges Author chain B； PDBConstruct 1–289； UniProt 1–289

MITORIBOSOMAL PROTEIN US3M, MRPS24

OrganismNot specified

UniProt F1ST79

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein–RNA Heteromer Protein × 33 RNA 4 PDB declaration: 37-meric（37） Consistent with all polymer counts Chain C； UniProt 1–167 Not recorded MITORIBOSOMAL 12S RRNA × 1 MITORIBOSOMAL PROTEIN US2M, MRPS2 × 1 （F1S001） MITORIBOSOMAL PROTEIN US5M, MRPS5 × 1 （F1SU66） MITORIBOSOMAL PROTEIN BS6M, MRPS6 × 1 MITORIBOSOMAL PROTEIN US7M, MRPS7 × 1 （F1RVX4） MITORIBOSOMAL PROTEIN US9M, MRPS9 × 1 MITORIBOSOMAL PROTEIN US10M, MRPS10 × 1 MITORIBOSOMAL PROTEIN US11M, MRPS11 × 1 （F1SRT1） MITORIBOSOMAL PROTEIN US12M, MRPS12 × 1 MITORIBOSOMAL PROTEIN US14M, MRPS14 × 1 MITORIBOSOMAL PROTEIN US15M, MRPS15 × 1 MITORIBOSOMAL PROTEIN BS16M, MRPS16 × 1 （F1SU70） MITORIBOSOMAL PROTEIN US17M, MRPS17 × 1 （F1RIU0） MITORIBOSOMAL PROTEIN BS18M, MRPS18C × 1 MITORIBOSOMAL PROTEIN BL19M, MRPL19 × 1 MITORIBOSOMAL PROTEIN BS21M, MRPS21 × 1 （I3L971） P-SITE AND A-SITE TRNA × 2 MRNA × 1 MITORIBOSOMAL PROTEIN MS22, MRPS22 × 1 MITORIBOSOMAL PROTEIN MS23, MRPS23 × 1 MITORIBOSOMAL PROTEIN MS25, MRPS25 × 1 MITORIBOSOMAL PROTEIN MS26, MRPS26 × 1 （F1S8A5） MITORIBOSOMAL PROTEIN MS27, MRPS27 × 1 MITORIBOSOMAL PROTEIN MS28, MRPS28 × 1 MITORIBOSOMAL PROTEIN MS29, MRPS29 × 1 MITORIBOSOMAL PROTEIN MS31, MRPS31 × 1 （F1RME2） MITORIBOSOMAL PROTEIN MS33, MRPS33 × 1 （F1S8G3） MITORIBOSOMAL PROTEIN MS34, MRPS34 × 1 MITORIBOSOMAL PROTEIN MS35, MRPS35 × 1 （F1SG95） MITORIBOSOMAL PROTEIN MS37, MRPS37 × 1 （F1SU49） MITORIBOSOMAL PROTEIN MS38, MRPS38 × 1 MITORIBOSOMAL PROTEIN MS39, MRPS39 × 1 （K7GKS8） MITORIBOSOMAL PROTEIN MS40, MRPS18B × 1 （Q767K8） UNASSIGNED HELICES × 1 UNASSIGNED HELICES × 1 MG MAGNESIUM ION × 144 ZN ZINC ION × 3 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY cryo-EM buffer：20 MM HEPES-KOH, 50 MM KCL, 40 MM MGCL2, 1 MM DTT；pH 7.4；20 MM HEPES-KOH, 50 MM KCL, 40 MM MGCL2, 1 MM DTT cryo-EM vitrification conditions：Cryogen ETHANE-PROPANE；MIXTURE OF LIQUID ETHANE AND PROPANE Resolution 3.60 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 5AJ3 Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
5AJ4 Structure of the 55S mammalian mitoribosome. Deposited 2015-02-20 Different construct Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 83 PDB declaration: 89-meric
Chain AC 36–167（132 aa）
Not recorded MG MAGNESIUM ION × 351 ZN ZINC ION × 6 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer 20 MM HEPES-KOH, 50 MM KCL, 40 MM MGCL2, 1 MM DTT；pH 7.4；20 MM HEPES-KOH, 50 MM KCL, 40 MM MGCL2, 1 MM DTT
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；MIXTURE OF LIQUID ETHANE AND PROPANE
Resolution 3.80 Å

1 other PDB entries and 1 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name F1ST79_PIG
Isoform —
PDB entities 3
Chains and sequence ranges Author chain C； PDBConstruct 1–167； UniProt 1–167

MITORIBOSOMAL PROTEIN US5M, MRPS5

OrganismNot specified

UniProt F1SU66

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein–RNA Heteromer Protein × 33 RNA 4 PDB declaration: 37-meric（37） Consistent with all polymer counts Chain E； UniProt 1–430 Not recorded MITORIBOSOMAL 12S RRNA × 1 MITORIBOSOMAL PROTEIN US2M, MRPS2 × 1 （F1S001） MITORIBOSOMAL PROTEIN US3M, MRPS24 × 1 （F1ST79） MITORIBOSOMAL PROTEIN BS6M, MRPS6 × 1 MITORIBOSOMAL PROTEIN US7M, MRPS7 × 1 （F1RVX4） MITORIBOSOMAL PROTEIN US9M, MRPS9 × 1 MITORIBOSOMAL PROTEIN US10M, MRPS10 × 1 MITORIBOSOMAL PROTEIN US11M, MRPS11 × 1 （F1SRT1） MITORIBOSOMAL PROTEIN US12M, MRPS12 × 1 MITORIBOSOMAL PROTEIN US14M, MRPS14 × 1 MITORIBOSOMAL PROTEIN US15M, MRPS15 × 1 MITORIBOSOMAL PROTEIN BS16M, MRPS16 × 1 （F1SU70） MITORIBOSOMAL PROTEIN US17M, MRPS17 × 1 （F1RIU0） MITORIBOSOMAL PROTEIN BS18M, MRPS18C × 1 MITORIBOSOMAL PROTEIN BL19M, MRPL19 × 1 MITORIBOSOMAL PROTEIN BS21M, MRPS21 × 1 （I3L971） P-SITE AND A-SITE TRNA × 2 MRNA × 1 MITORIBOSOMAL PROTEIN MS22, MRPS22 × 1 MITORIBOSOMAL PROTEIN MS23, MRPS23 × 1 MITORIBOSOMAL PROTEIN MS25, MRPS25 × 1 MITORIBOSOMAL PROTEIN MS26, MRPS26 × 1 （F1S8A5） MITORIBOSOMAL PROTEIN MS27, MRPS27 × 1 MITORIBOSOMAL PROTEIN MS28, MRPS28 × 1 MITORIBOSOMAL PROTEIN MS29, MRPS29 × 1 MITORIBOSOMAL PROTEIN MS31, MRPS31 × 1 （F1RME2） MITORIBOSOMAL PROTEIN MS33, MRPS33 × 1 （F1S8G3） MITORIBOSOMAL PROTEIN MS34, MRPS34 × 1 MITORIBOSOMAL PROTEIN MS35, MRPS35 × 1 （F1SG95） MITORIBOSOMAL PROTEIN MS37, MRPS37 × 1 （F1SU49） MITORIBOSOMAL PROTEIN MS38, MRPS38 × 1 MITORIBOSOMAL PROTEIN MS39, MRPS39 × 1 （K7GKS8） MITORIBOSOMAL PROTEIN MS40, MRPS18B × 1 （Q767K8） UNASSIGNED HELICES × 1 UNASSIGNED HELICES × 1 MG MAGNESIUM ION × 144 ZN ZINC ION × 3 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY cryo-EM buffer：20 MM HEPES-KOH, 50 MM KCL, 40 MM MGCL2, 1 MM DTT；pH 7.4；20 MM HEPES-KOH, 50 MM KCL, 40 MM MGCL2, 1 MM DTT cryo-EM vitrification conditions：Cryogen ETHANE-PROPANE；MIXTURE OF LIQUID ETHANE AND PROPANE Resolution 3.60 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 5AJ3 Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
5AJ4 Structure of the 55S mammalian mitoribosome. Deposited 2015-02-20 Different construct Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 83 PDB declaration: 89-meric
Chain AE 88–430（343 aa）
Not recorded MG MAGNESIUM ION × 351 ZN ZINC ION × 6 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer 20 MM HEPES-KOH, 50 MM KCL, 40 MM MGCL2, 1 MM DTT；pH 7.4；20 MM HEPES-KOH, 50 MM KCL, 40 MM MGCL2, 1 MM DTT
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；MIXTURE OF LIQUID ETHANE AND PROPANE
Resolution 3.80 Å
6GAW Unique features of mammalian mitochondrial translation initiation revealed by cryo-EM. This file contains the complete 55S ribosome. Deposited 2018-04-13 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 90 PDB declaration: 95-meric
Chain AE 1–430（430 aa）
Not recorded MG MAGNESIUM ION × 326 ZN ZINC ION × 6 5GP GUANOSINE-5'-MONOPHOSPHATE × 2 SPM SPERMINE × 3 GSP 5'-GUANOSINE-DIPHOSPHATE-MONOTHIOPHOSPHATE × 1 NA SODIUM ION × 1 FME N-FORMYLMETHIONINE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
6GAZ Unique features of mammalian mitochondrial translation initiation revealed by cryo-EM. This file contains the 28S ribosomal subunit. Deposited 2018-04-13 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 33 PDB declaration: 36-meric
Chain AE 1–430（430 aa）
Not recorded GSP 5'-GUANOSINE-DIPHOSPHATE-MONOTHIOPHOSPHATE × 1 MG MAGNESIUM ION × 111 NA SODIUM ION × 1 SPM SPERMINE × 1 ZN ZINC ION × 3 FME N-FORMYLMETHIONINE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
6YDP 55S mammalian mitochondrial ribosome with mtEFG1 and P site fMet-tRNAMet (POST) Deposited 2020-03-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 89 PDB declaration: 94-meric
Chain AE 1–430（430 aa）
Not recorded MG MAGNESIUM ION × 326 ZN ZINC ION × 6 SPM SPERMINE × 3 FME N-FORMYLMETHIONINE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 5GP GUANOSINE-5'-MONOPHOSPHATE × 2 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.00 Å
6YDW 55S mammalian mitochondrial ribosome with mtEFG1 and two tRNAMet (TI-POST) Deposited 2020-03-21 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 89 PDB declaration: 95-meric
Chain AE 1–430（430 aa）
Not recorded MG MAGNESIUM ION × 325 ZN ZINC ION × 6 SPM SPERMINE × 3 FME N-FORMYLMETHIONINE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 5GP GUANOSINE-5'-MONOPHOSPHATE × 1 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 4.20 Å
7NQH 55S mammalian mitochondrial ribosome with mtRF1a and P-site tRNAMet Deposited 2021-03-01 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 88 PDB declaration: 93-meric
Chain AE 1–430（430 aa）
Not recorded MG MAGNESIUM ION × 323 ZN ZINC ION × 6 SPM SPERMINE × 3 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 5GP GUANOSINE-5'-MONOPHOSPHATE × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.50 Å
7NQL 55S mammalian mitochondrial ribosome with ICT1 and P site tRNAMet Deposited 2021-03-01 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 88 PDB declaration: 93-meric
Chain AE 1–430（430 aa）
Not recorded ZN ZINC ION × 6 MG MAGNESIUM ION × 335 5GP GUANOSINE-5'-MONOPHOSPHATE × 2 SPM SPERMINE × 3 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.40 Å
7NSI 55S mammalian mitochondrial ribosome with mtRRF (pre) and tRNA(P/E) Deposited 2021-03-07 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 88 PDB declaration: 93-meric
Chain AE 1–430（430 aa）
Not recorded MG MAGNESIUM ION × 327 ZN ZINC ION × 6 SPM SPERMINE × 3 5GP GUANOSINE-5'-MONOPHOSPHATE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 4.60 Å
7NSJ 55S mammalian mitochondrial ribosome with tRNA(P/P) and tRNA(E*) Deposited 2021-03-07 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 87 PDB declaration: 93-meric
Chain AE 1–430（430 aa）
Not recorded MG MAGNESIUM ION × 324 ZN ZINC ION × 6 SPM SPERMINE × 3 5GP GUANOSINE-5'-MONOPHOSPHATE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.90 Å
8OIN 55S mammalian mitochondrial ribosome with mtRF1 and P-site tRNA Deposited 2023-03-23 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 88 PDB declaration: 94-meric
Chain Ad 1–430（430 aa）
Not recorded MG MAGNESIUM ION × 332 K POTASSIUM ION × 41 PHE PHENYLALANINE × 1 ZN ZINC ION × 3 FES FE2/S2 (INORGANIC) CLUSTER × 3 SPM SPERMINE × 1 SPD SPERMIDINE × 1 FME N-FORMYLMETHIONINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20mM HEPES-KOH pH=7.5, 100mM KCl, 20mM MgCl2, ~1% sucrose (from mtRF1 storage buffer)
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.60 Å
8OIP 28S mammalian mitochondrial small ribosomal subunit with mtRF1 and P-site tRNA Deposited 2023-03-23 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 33 PDB declaration: 36-meric
Chain Ad 1–430（430 aa）
Not recorded MG MAGNESIUM ION × 127 SPM SPERMINE × 1 SPD SPERMIDINE × 1 K POTASSIUM ION × 11 ZN ZINC ION × 1 FS2 FE-S-O HYBRID CLUSTER × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20mM HEPES-KOH pH=7.5, 100mM KCl, 20mM MgCl2, ~1% sucrose (from mtRF1 storage buffer)
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.60 Å

11 other PDB entries and 11 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name F1SU66_PIG
Isoform —
PDB entities 4
Chains and sequence ranges Author chain E； PDBConstruct 1–430； UniProt 1–430

MITORIBOSOMAL PROTEIN US7M, MRPS7

OrganismNot specified

UniProt F1RVX4

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein–RNA Heteromer Protein × 33 RNA 4 PDB declaration: 37-meric（37） Consistent with all polymer counts Chain G； UniProt 1–242 Not recorded MITORIBOSOMAL 12S RRNA × 1 MITORIBOSOMAL PROTEIN US2M, MRPS2 × 1 （F1S001） MITORIBOSOMAL PROTEIN US3M, MRPS24 × 1 （F1ST79） MITORIBOSOMAL PROTEIN US5M, MRPS5 × 1 （F1SU66） MITORIBOSOMAL PROTEIN BS6M, MRPS6 × 1 MITORIBOSOMAL PROTEIN US9M, MRPS9 × 1 MITORIBOSOMAL PROTEIN US10M, MRPS10 × 1 MITORIBOSOMAL PROTEIN US11M, MRPS11 × 1 （F1SRT1） MITORIBOSOMAL PROTEIN US12M, MRPS12 × 1 MITORIBOSOMAL PROTEIN US14M, MRPS14 × 1 MITORIBOSOMAL PROTEIN US15M, MRPS15 × 1 MITORIBOSOMAL PROTEIN BS16M, MRPS16 × 1 （F1SU70） MITORIBOSOMAL PROTEIN US17M, MRPS17 × 1 （F1RIU0） MITORIBOSOMAL PROTEIN BS18M, MRPS18C × 1 MITORIBOSOMAL PROTEIN BL19M, MRPL19 × 1 MITORIBOSOMAL PROTEIN BS21M, MRPS21 × 1 （I3L971） P-SITE AND A-SITE TRNA × 2 MRNA × 1 MITORIBOSOMAL PROTEIN MS22, MRPS22 × 1 MITORIBOSOMAL PROTEIN MS23, MRPS23 × 1 MITORIBOSOMAL PROTEIN MS25, MRPS25 × 1 MITORIBOSOMAL PROTEIN MS26, MRPS26 × 1 （F1S8A5） MITORIBOSOMAL PROTEIN MS27, MRPS27 × 1 MITORIBOSOMAL PROTEIN MS28, MRPS28 × 1 MITORIBOSOMAL PROTEIN MS29, MRPS29 × 1 MITORIBOSOMAL PROTEIN MS31, MRPS31 × 1 （F1RME2） MITORIBOSOMAL PROTEIN MS33, MRPS33 × 1 （F1S8G3） MITORIBOSOMAL PROTEIN MS34, MRPS34 × 1 MITORIBOSOMAL PROTEIN MS35, MRPS35 × 1 （F1SG95） MITORIBOSOMAL PROTEIN MS37, MRPS37 × 1 （F1SU49） MITORIBOSOMAL PROTEIN MS38, MRPS38 × 1 MITORIBOSOMAL PROTEIN MS39, MRPS39 × 1 （K7GKS8） MITORIBOSOMAL PROTEIN MS40, MRPS18B × 1 （Q767K8） UNASSIGNED HELICES × 1 UNASSIGNED HELICES × 1 MG MAGNESIUM ION × 144 ZN ZINC ION × 3 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY cryo-EM buffer：20 MM HEPES-KOH, 50 MM KCL, 40 MM MGCL2, 1 MM DTT；pH 7.4；20 MM HEPES-KOH, 50 MM KCL, 40 MM MGCL2, 1 MM DTT cryo-EM vitrification conditions：Cryogen ETHANE-PROPANE；MIXTURE OF LIQUID ETHANE AND PROPANE Resolution 3.60 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 5AJ3 Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
5AJ4 Structure of the 55S mammalian mitoribosome. Deposited 2015-02-20 Different construct Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 83 PDB declaration: 89-meric
Chain AG 35–242（208 aa）
Not recorded MG MAGNESIUM ION × 351 ZN ZINC ION × 6 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer 20 MM HEPES-KOH, 50 MM KCL, 40 MM MGCL2, 1 MM DTT；pH 7.4；20 MM HEPES-KOH, 50 MM KCL, 40 MM MGCL2, 1 MM DTT
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；MIXTURE OF LIQUID ETHANE AND PROPANE
Resolution 3.80 Å

1 other PDB entries and 1 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name F1RVX4_PIG
Isoform —
PDB entities 6
Chains and sequence ranges Author chain G； PDBConstruct 1–242； UniProt 1–242

MITORIBOSOMAL PROTEIN US11M, MRPS11

OrganismNot specified

UniProt F1SRT1

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein–RNA Heteromer Protein × 33 RNA 4 PDB declaration: 37-meric（37） Consistent with all polymer counts Chain K； UniProt 1–196 Not recorded MITORIBOSOMAL 12S RRNA × 1 MITORIBOSOMAL PROTEIN US2M, MRPS2 × 1 （F1S001） MITORIBOSOMAL PROTEIN US3M, MRPS24 × 1 （F1ST79） MITORIBOSOMAL PROTEIN US5M, MRPS5 × 1 （F1SU66） MITORIBOSOMAL PROTEIN BS6M, MRPS6 × 1 MITORIBOSOMAL PROTEIN US7M, MRPS7 × 1 （F1RVX4） MITORIBOSOMAL PROTEIN US9M, MRPS9 × 1 MITORIBOSOMAL PROTEIN US10M, MRPS10 × 1 MITORIBOSOMAL PROTEIN US12M, MRPS12 × 1 MITORIBOSOMAL PROTEIN US14M, MRPS14 × 1 MITORIBOSOMAL PROTEIN US15M, MRPS15 × 1 MITORIBOSOMAL PROTEIN BS16M, MRPS16 × 1 （F1SU70） MITORIBOSOMAL PROTEIN US17M, MRPS17 × 1 （F1RIU0） MITORIBOSOMAL PROTEIN BS18M, MRPS18C × 1 MITORIBOSOMAL PROTEIN BL19M, MRPL19 × 1 MITORIBOSOMAL PROTEIN BS21M, MRPS21 × 1 （I3L971） P-SITE AND A-SITE TRNA × 2 MRNA × 1 MITORIBOSOMAL PROTEIN MS22, MRPS22 × 1 MITORIBOSOMAL PROTEIN MS23, MRPS23 × 1 MITORIBOSOMAL PROTEIN MS25, MRPS25 × 1 MITORIBOSOMAL PROTEIN MS26, MRPS26 × 1 （F1S8A5） MITORIBOSOMAL PROTEIN MS27, MRPS27 × 1 MITORIBOSOMAL PROTEIN MS28, MRPS28 × 1 MITORIBOSOMAL PROTEIN MS29, MRPS29 × 1 MITORIBOSOMAL PROTEIN MS31, MRPS31 × 1 （F1RME2） MITORIBOSOMAL PROTEIN MS33, MRPS33 × 1 （F1S8G3） MITORIBOSOMAL PROTEIN MS34, MRPS34 × 1 MITORIBOSOMAL PROTEIN MS35, MRPS35 × 1 （F1SG95） MITORIBOSOMAL PROTEIN MS37, MRPS37 × 1 （F1SU49） MITORIBOSOMAL PROTEIN MS38, MRPS38 × 1 MITORIBOSOMAL PROTEIN MS39, MRPS39 × 1 （K7GKS8） MITORIBOSOMAL PROTEIN MS40, MRPS18B × 1 （Q767K8） UNASSIGNED HELICES × 1 UNASSIGNED HELICES × 1 MG MAGNESIUM ION × 144 ZN ZINC ION × 3 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY cryo-EM buffer：20 MM HEPES-KOH, 50 MM KCL, 40 MM MGCL2, 1 MM DTT；pH 7.4；20 MM HEPES-KOH, 50 MM KCL, 40 MM MGCL2, 1 MM DTT cryo-EM vitrification conditions：Cryogen ETHANE-PROPANE；MIXTURE OF LIQUID ETHANE AND PROPANE Resolution 3.60 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 5AJ3 Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
5AJ4 Structure of the 55S mammalian mitoribosome. Deposited 2015-02-20 Different construct Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 83 PDB declaration: 89-meric
Chain AK 61–196（136 aa）
Not recorded MG MAGNESIUM ION × 351 ZN ZINC ION × 6 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer 20 MM HEPES-KOH, 50 MM KCL, 40 MM MGCL2, 1 MM DTT；pH 7.4；20 MM HEPES-KOH, 50 MM KCL, 40 MM MGCL2, 1 MM DTT
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；MIXTURE OF LIQUID ETHANE AND PROPANE
Resolution 3.80 Å

1 other PDB entries and 1 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name F1SRT1_PIG
Isoform —
PDB entities 9
Chains and sequence ranges Author chain K； PDBConstruct 1–196； UniProt 1–196

MITORIBOSOMAL PROTEIN BS16M, MRPS16

OrganismNot specified

UniProt F1SU70

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein–RNA Heteromer Protein × 33 RNA 4 PDB declaration: 37-meric（37） Consistent with all polymer counts Chain P； UniProt 1–135 Not recorded MITORIBOSOMAL 12S RRNA × 1 MITORIBOSOMAL PROTEIN US2M, MRPS2 × 1 （F1S001） MITORIBOSOMAL PROTEIN US3M, MRPS24 × 1 （F1ST79） MITORIBOSOMAL PROTEIN US5M, MRPS5 × 1 （F1SU66） MITORIBOSOMAL PROTEIN BS6M, MRPS6 × 1 MITORIBOSOMAL PROTEIN US7M, MRPS7 × 1 （F1RVX4） MITORIBOSOMAL PROTEIN US9M, MRPS9 × 1 MITORIBOSOMAL PROTEIN US10M, MRPS10 × 1 MITORIBOSOMAL PROTEIN US11M, MRPS11 × 1 （F1SRT1） MITORIBOSOMAL PROTEIN US12M, MRPS12 × 1 MITORIBOSOMAL PROTEIN US14M, MRPS14 × 1 MITORIBOSOMAL PROTEIN US15M, MRPS15 × 1 MITORIBOSOMAL PROTEIN US17M, MRPS17 × 1 （F1RIU0） MITORIBOSOMAL PROTEIN BS18M, MRPS18C × 1 MITORIBOSOMAL PROTEIN BL19M, MRPL19 × 1 MITORIBOSOMAL PROTEIN BS21M, MRPS21 × 1 （I3L971） P-SITE AND A-SITE TRNA × 2 MRNA × 1 MITORIBOSOMAL PROTEIN MS22, MRPS22 × 1 MITORIBOSOMAL PROTEIN MS23, MRPS23 × 1 MITORIBOSOMAL PROTEIN MS25, MRPS25 × 1 MITORIBOSOMAL PROTEIN MS26, MRPS26 × 1 （F1S8A5） MITORIBOSOMAL PROTEIN MS27, MRPS27 × 1 MITORIBOSOMAL PROTEIN MS28, MRPS28 × 1 MITORIBOSOMAL PROTEIN MS29, MRPS29 × 1 MITORIBOSOMAL PROTEIN MS31, MRPS31 × 1 （F1RME2） MITORIBOSOMAL PROTEIN MS33, MRPS33 × 1 （F1S8G3） MITORIBOSOMAL PROTEIN MS34, MRPS34 × 1 MITORIBOSOMAL PROTEIN MS35, MRPS35 × 1 （F1SG95） MITORIBOSOMAL PROTEIN MS37, MRPS37 × 1 （F1SU49） MITORIBOSOMAL PROTEIN MS38, MRPS38 × 1 MITORIBOSOMAL PROTEIN MS39, MRPS39 × 1 （K7GKS8） MITORIBOSOMAL PROTEIN MS40, MRPS18B × 1 （Q767K8） UNASSIGNED HELICES × 1 UNASSIGNED HELICES × 1 MG MAGNESIUM ION × 144 ZN ZINC ION × 3 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY cryo-EM buffer：20 MM HEPES-KOH, 50 MM KCL, 40 MM MGCL2, 1 MM DTT；pH 7.4；20 MM HEPES-KOH, 50 MM KCL, 40 MM MGCL2, 1 MM DTT cryo-EM vitrification conditions：Cryogen ETHANE-PROPANE；MIXTURE OF LIQUID ETHANE AND PROPANE Resolution 3.60 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 5AJ3 Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
5AJ4 Structure of the 55S mammalian mitoribosome. Deposited 2015-02-20 Different construct Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 83 PDB declaration: 89-meric
Chain AP 10–126（117 aa）
Not recorded MG MAGNESIUM ION × 351 ZN ZINC ION × 6 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer 20 MM HEPES-KOH, 50 MM KCL, 40 MM MGCL2, 1 MM DTT；pH 7.4；20 MM HEPES-KOH, 50 MM KCL, 40 MM MGCL2, 1 MM DTT
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；MIXTURE OF LIQUID ETHANE AND PROPANE
Resolution 3.80 Å

1 other PDB entries and 1 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name F1SU70_PIG
Isoform —
PDB entities 13
Chains and sequence ranges Author chain P； PDBConstruct 1–135； UniProt 1–135

MITORIBOSOMAL PROTEIN US17M, MRPS17

OrganismNot specified

UniProt F1RIU0

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein–RNA Heteromer Protein × 33 RNA 4 PDB declaration: 37-meric（37） Consistent with all polymer counts Chain Q； UniProt 1–130 Not recorded MITORIBOSOMAL 12S RRNA × 1 MITORIBOSOMAL PROTEIN US2M, MRPS2 × 1 （F1S001） MITORIBOSOMAL PROTEIN US3M, MRPS24 × 1 （F1ST79） MITORIBOSOMAL PROTEIN US5M, MRPS5 × 1 （F1SU66） MITORIBOSOMAL PROTEIN BS6M, MRPS6 × 1 MITORIBOSOMAL PROTEIN US7M, MRPS7 × 1 （F1RVX4） MITORIBOSOMAL PROTEIN US9M, MRPS9 × 1 MITORIBOSOMAL PROTEIN US10M, MRPS10 × 1 MITORIBOSOMAL PROTEIN US11M, MRPS11 × 1 （F1SRT1） MITORIBOSOMAL PROTEIN US12M, MRPS12 × 1 MITORIBOSOMAL PROTEIN US14M, MRPS14 × 1 MITORIBOSOMAL PROTEIN US15M, MRPS15 × 1 MITORIBOSOMAL PROTEIN BS16M, MRPS16 × 1 （F1SU70） MITORIBOSOMAL PROTEIN BS18M, MRPS18C × 1 MITORIBOSOMAL PROTEIN BL19M, MRPL19 × 1 MITORIBOSOMAL PROTEIN BS21M, MRPS21 × 1 （I3L971） P-SITE AND A-SITE TRNA × 2 MRNA × 1 MITORIBOSOMAL PROTEIN MS22, MRPS22 × 1 MITORIBOSOMAL PROTEIN MS23, MRPS23 × 1 MITORIBOSOMAL PROTEIN MS25, MRPS25 × 1 MITORIBOSOMAL PROTEIN MS26, MRPS26 × 1 （F1S8A5） MITORIBOSOMAL PROTEIN MS27, MRPS27 × 1 MITORIBOSOMAL PROTEIN MS28, MRPS28 × 1 MITORIBOSOMAL PROTEIN MS29, MRPS29 × 1 MITORIBOSOMAL PROTEIN MS31, MRPS31 × 1 （F1RME2） MITORIBOSOMAL PROTEIN MS33, MRPS33 × 1 （F1S8G3） MITORIBOSOMAL PROTEIN MS34, MRPS34 × 1 MITORIBOSOMAL PROTEIN MS35, MRPS35 × 1 （F1SG95） MITORIBOSOMAL PROTEIN MS37, MRPS37 × 1 （F1SU49） MITORIBOSOMAL PROTEIN MS38, MRPS38 × 1 MITORIBOSOMAL PROTEIN MS39, MRPS39 × 1 （K7GKS8） MITORIBOSOMAL PROTEIN MS40, MRPS18B × 1 （Q767K8） UNASSIGNED HELICES × 1 UNASSIGNED HELICES × 1 MG MAGNESIUM ION × 144 ZN ZINC ION × 3 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY cryo-EM buffer：20 MM HEPES-KOH, 50 MM KCL, 40 MM MGCL2, 1 MM DTT；pH 7.4；20 MM HEPES-KOH, 50 MM KCL, 40 MM MGCL2, 1 MM DTT cryo-EM vitrification conditions：Cryogen ETHANE-PROPANE；MIXTURE OF LIQUID ETHANE AND PROPANE Resolution 3.60 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 5AJ3 Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
5AJ4 Structure of the 55S mammalian mitoribosome. Deposited 2015-02-20 Different construct Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 83 PDB declaration: 89-meric
Chain AQ 2–110（109 aa）
Not recorded MG MAGNESIUM ION × 351 ZN ZINC ION × 6 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer 20 MM HEPES-KOH, 50 MM KCL, 40 MM MGCL2, 1 MM DTT；pH 7.4；20 MM HEPES-KOH, 50 MM KCL, 40 MM MGCL2, 1 MM DTT
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；MIXTURE OF LIQUID ETHANE AND PROPANE
Resolution 3.80 Å
6GAW Unique features of mammalian mitochondrial translation initiation revealed by cryo-EM. This file contains the complete 55S ribosome. Deposited 2018-04-13 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 90 PDB declaration: 95-meric
Chain AQ 1–130（130 aa）
Not recorded MG MAGNESIUM ION × 326 ZN ZINC ION × 6 5GP GUANOSINE-5'-MONOPHOSPHATE × 2 SPM SPERMINE × 3 GSP 5'-GUANOSINE-DIPHOSPHATE-MONOTHIOPHOSPHATE × 1 NA SODIUM ION × 1 FME N-FORMYLMETHIONINE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
6GAZ Unique features of mammalian mitochondrial translation initiation revealed by cryo-EM. This file contains the 28S ribosomal subunit. Deposited 2018-04-13 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 33 PDB declaration: 36-meric
Chain AQ 1–130（130 aa）
Not recorded GSP 5'-GUANOSINE-DIPHOSPHATE-MONOTHIOPHOSPHATE × 1 MG MAGNESIUM ION × 111 NA SODIUM ION × 1 SPM SPERMINE × 1 ZN ZINC ION × 3 FME N-FORMYLMETHIONINE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
6YDP 55S mammalian mitochondrial ribosome with mtEFG1 and P site fMet-tRNAMet (POST) Deposited 2020-03-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 89 PDB declaration: 94-meric
Chain AQ 1–130（130 aa）
Not recorded MG MAGNESIUM ION × 326 ZN ZINC ION × 6 SPM SPERMINE × 3 FME N-FORMYLMETHIONINE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 5GP GUANOSINE-5'-MONOPHOSPHATE × 2 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.00 Å
6YDW 55S mammalian mitochondrial ribosome with mtEFG1 and two tRNAMet (TI-POST) Deposited 2020-03-21 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 89 PDB declaration: 95-meric
Chain AQ 1–130（130 aa）
Not recorded MG MAGNESIUM ION × 325 ZN ZINC ION × 6 SPM SPERMINE × 3 FME N-FORMYLMETHIONINE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 5GP GUANOSINE-5'-MONOPHOSPHATE × 1 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 4.20 Å
7NQH 55S mammalian mitochondrial ribosome with mtRF1a and P-site tRNAMet Deposited 2021-03-01 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 88 PDB declaration: 93-meric
Chain AQ 1–130（130 aa）
Not recorded MG MAGNESIUM ION × 323 ZN ZINC ION × 6 SPM SPERMINE × 3 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 5GP GUANOSINE-5'-MONOPHOSPHATE × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.50 Å
7NQL 55S mammalian mitochondrial ribosome with ICT1 and P site tRNAMet Deposited 2021-03-01 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 88 PDB declaration: 93-meric
Chain AQ 1–130（130 aa）
Not recorded ZN ZINC ION × 6 MG MAGNESIUM ION × 335 5GP GUANOSINE-5'-MONOPHOSPHATE × 2 SPM SPERMINE × 3 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.40 Å
7NSI 55S mammalian mitochondrial ribosome with mtRRF (pre) and tRNA(P/E) Deposited 2021-03-07 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 88 PDB declaration: 93-meric
Chain AQ 1–130（130 aa）
Not recorded MG MAGNESIUM ION × 327 ZN ZINC ION × 6 SPM SPERMINE × 3 5GP GUANOSINE-5'-MONOPHOSPHATE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 4.60 Å
7NSJ 55S mammalian mitochondrial ribosome with tRNA(P/P) and tRNA(E*) Deposited 2021-03-07 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 87 PDB declaration: 93-meric
Chain AQ 1–130（130 aa）
Not recorded MG MAGNESIUM ION × 324 ZN ZINC ION × 6 SPM SPERMINE × 3 5GP GUANOSINE-5'-MONOPHOSPHATE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.90 Å
8OIP 28S mammalian mitochondrial small ribosomal subunit with mtRF1 and P-site tRNA Deposited 2023-03-23 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 33 PDB declaration: 36-meric
Chain AN 1–130（130 aa）
Not recorded MG MAGNESIUM ION × 127 SPM SPERMINE × 1 SPD SPERMIDINE × 1 K POTASSIUM ION × 11 ZN ZINC ION × 1 FS2 FE-S-O HYBRID CLUSTER × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20mM HEPES-KOH pH=7.5, 100mM KCl, 20mM MgCl2, ~1% sucrose (from mtRF1 storage buffer)
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.60 Å

10 other PDB entries and 10 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name F1RIU0_PIG
Isoform —
PDB entities 14
Chains and sequence ranges Author chain Q； PDBConstruct 1–130； UniProt 1–130

MITORIBOSOMAL PROTEIN BS21M, MRPS21

OrganismNot specified

UniProt I3L971

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein–RNA Heteromer Protein × 33 RNA 4 PDB declaration: 37-meric（37） Consistent with all polymer counts Chain U； UniProt 1–87 Not recorded MITORIBOSOMAL 12S RRNA × 1 MITORIBOSOMAL PROTEIN US2M, MRPS2 × 1 （F1S001） MITORIBOSOMAL PROTEIN US3M, MRPS24 × 1 （F1ST79） MITORIBOSOMAL PROTEIN US5M, MRPS5 × 1 （F1SU66） MITORIBOSOMAL PROTEIN BS6M, MRPS6 × 1 MITORIBOSOMAL PROTEIN US7M, MRPS7 × 1 （F1RVX4） MITORIBOSOMAL PROTEIN US9M, MRPS9 × 1 MITORIBOSOMAL PROTEIN US10M, MRPS10 × 1 MITORIBOSOMAL PROTEIN US11M, MRPS11 × 1 （F1SRT1） MITORIBOSOMAL PROTEIN US12M, MRPS12 × 1 MITORIBOSOMAL PROTEIN US14M, MRPS14 × 1 MITORIBOSOMAL PROTEIN US15M, MRPS15 × 1 MITORIBOSOMAL PROTEIN BS16M, MRPS16 × 1 （F1SU70） MITORIBOSOMAL PROTEIN US17M, MRPS17 × 1 （F1RIU0） MITORIBOSOMAL PROTEIN BS18M, MRPS18C × 1 MITORIBOSOMAL PROTEIN BL19M, MRPL19 × 1 P-SITE AND A-SITE TRNA × 2 MRNA × 1 MITORIBOSOMAL PROTEIN MS22, MRPS22 × 1 MITORIBOSOMAL PROTEIN MS23, MRPS23 × 1 MITORIBOSOMAL PROTEIN MS25, MRPS25 × 1 MITORIBOSOMAL PROTEIN MS26, MRPS26 × 1 （F1S8A5） MITORIBOSOMAL PROTEIN MS27, MRPS27 × 1 MITORIBOSOMAL PROTEIN MS28, MRPS28 × 1 MITORIBOSOMAL PROTEIN MS29, MRPS29 × 1 MITORIBOSOMAL PROTEIN MS31, MRPS31 × 1 （F1RME2） MITORIBOSOMAL PROTEIN MS33, MRPS33 × 1 （F1S8G3） MITORIBOSOMAL PROTEIN MS34, MRPS34 × 1 MITORIBOSOMAL PROTEIN MS35, MRPS35 × 1 （F1SG95） MITORIBOSOMAL PROTEIN MS37, MRPS37 × 1 （F1SU49） MITORIBOSOMAL PROTEIN MS38, MRPS38 × 1 MITORIBOSOMAL PROTEIN MS39, MRPS39 × 1 （K7GKS8） MITORIBOSOMAL PROTEIN MS40, MRPS18B × 1 （Q767K8） UNASSIGNED HELICES × 1 UNASSIGNED HELICES × 1 MG MAGNESIUM ION × 144 ZN ZINC ION × 3 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY cryo-EM buffer：20 MM HEPES-KOH, 50 MM KCL, 40 MM MGCL2, 1 MM DTT；pH 7.4；20 MM HEPES-KOH, 50 MM KCL, 40 MM MGCL2, 1 MM DTT cryo-EM vitrification conditions：Cryogen ETHANE-PROPANE；MIXTURE OF LIQUID ETHANE AND PROPANE Resolution 3.60 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 5AJ3 Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
5AJ4 Structure of the 55S mammalian mitoribosome. Deposited 2015-02-20 Different construct Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 83 PDB declaration: 89-meric
Chain AU 2–87（86 aa）
Not recorded MG MAGNESIUM ION × 351 ZN ZINC ION × 6 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer 20 MM HEPES-KOH, 50 MM KCL, 40 MM MGCL2, 1 MM DTT；pH 7.4；20 MM HEPES-KOH, 50 MM KCL, 40 MM MGCL2, 1 MM DTT
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；MIXTURE OF LIQUID ETHANE AND PROPANE
Resolution 3.80 Å

1 other PDB entries and 1 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name I3L971_PIG
Isoform —
PDB entities 17
Chains and sequence ranges Author chain U； PDBConstruct 1–87； UniProt 1–87

MITORIBOSOMAL PROTEIN MS26, MRPS26

OrganismNot specified

UniProt F1S8A5

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein–RNA Heteromer Protein × 33 RNA 4 PDB declaration: 37-meric（37） Consistent with all polymer counts Chain d； UniProt 1–205 Not recorded MITORIBOSOMAL 12S RRNA × 1 MITORIBOSOMAL PROTEIN US2M, MRPS2 × 1 （F1S001） MITORIBOSOMAL PROTEIN US3M, MRPS24 × 1 （F1ST79） MITORIBOSOMAL PROTEIN US5M, MRPS5 × 1 （F1SU66） MITORIBOSOMAL PROTEIN BS6M, MRPS6 × 1 MITORIBOSOMAL PROTEIN US7M, MRPS7 × 1 （F1RVX4） MITORIBOSOMAL PROTEIN US9M, MRPS9 × 1 MITORIBOSOMAL PROTEIN US10M, MRPS10 × 1 MITORIBOSOMAL PROTEIN US11M, MRPS11 × 1 （F1SRT1） MITORIBOSOMAL PROTEIN US12M, MRPS12 × 1 MITORIBOSOMAL PROTEIN US14M, MRPS14 × 1 MITORIBOSOMAL PROTEIN US15M, MRPS15 × 1 MITORIBOSOMAL PROTEIN BS16M, MRPS16 × 1 （F1SU70） MITORIBOSOMAL PROTEIN US17M, MRPS17 × 1 （F1RIU0） MITORIBOSOMAL PROTEIN BS18M, MRPS18C × 1 MITORIBOSOMAL PROTEIN BL19M, MRPL19 × 1 MITORIBOSOMAL PROTEIN BS21M, MRPS21 × 1 （I3L971） P-SITE AND A-SITE TRNA × 2 MRNA × 1 MITORIBOSOMAL PROTEIN MS22, MRPS22 × 1 MITORIBOSOMAL PROTEIN MS23, MRPS23 × 1 MITORIBOSOMAL PROTEIN MS25, MRPS25 × 1 MITORIBOSOMAL PROTEIN MS27, MRPS27 × 1 MITORIBOSOMAL PROTEIN MS28, MRPS28 × 1 MITORIBOSOMAL PROTEIN MS29, MRPS29 × 1 MITORIBOSOMAL PROTEIN MS31, MRPS31 × 1 （F1RME2） MITORIBOSOMAL PROTEIN MS33, MRPS33 × 1 （F1S8G3） MITORIBOSOMAL PROTEIN MS34, MRPS34 × 1 MITORIBOSOMAL PROTEIN MS35, MRPS35 × 1 （F1SG95） MITORIBOSOMAL PROTEIN MS37, MRPS37 × 1 （F1SU49） MITORIBOSOMAL PROTEIN MS38, MRPS38 × 1 MITORIBOSOMAL PROTEIN MS39, MRPS39 × 1 （K7GKS8） MITORIBOSOMAL PROTEIN MS40, MRPS18B × 1 （Q767K8） UNASSIGNED HELICES × 1 UNASSIGNED HELICES × 1 MG MAGNESIUM ION × 144 ZN ZINC ION × 3 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY cryo-EM buffer：20 MM HEPES-KOH, 50 MM KCL, 40 MM MGCL2, 1 MM DTT；pH 7.4；20 MM HEPES-KOH, 50 MM KCL, 40 MM MGCL2, 1 MM DTT cryo-EM vitrification conditions：Cryogen ETHANE-PROPANE；MIXTURE OF LIQUID ETHANE AND PROPANE Resolution 3.60 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 5AJ3 Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
5AJ4 Structure of the 55S mammalian mitoribosome. Deposited 2015-02-20 Different construct Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 83 PDB declaration: 89-meric
Chain Ad 27–203（177 aa）
Not recorded MG MAGNESIUM ION × 351 ZN ZINC ION × 6 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer 20 MM HEPES-KOH, 50 MM KCL, 40 MM MGCL2, 1 MM DTT；pH 7.4；20 MM HEPES-KOH, 50 MM KCL, 40 MM MGCL2, 1 MM DTT
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；MIXTURE OF LIQUID ETHANE AND PROPANE
Resolution 3.80 Å
7NSI 55S mammalian mitochondrial ribosome with mtRRF (pre) and tRNA(P/E) Deposited 2021-03-07 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 88 PDB declaration: 93-meric
Chain Ad 1–205（205 aa）
Not recorded MG MAGNESIUM ION × 327 ZN ZINC ION × 6 SPM SPERMINE × 3 5GP GUANOSINE-5'-MONOPHOSPHATE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 4.60 Å

2 other PDB entries and 2 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name F1S8A5_PIG
Isoform —
PDB entities 23
Chains and sequence ranges Author chain d； PDBConstruct 1–205； UniProt 1–205

MITORIBOSOMAL PROTEIN MS31, MRPS31

OrganismNot specified

UniProt F1RME2

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein–RNA Heteromer Protein × 33 RNA 4 PDB declaration: 37-meric（37） Consistent with all polymer counts Chain h； UniProt 1–387 Not recorded MITORIBOSOMAL 12S RRNA × 1 MITORIBOSOMAL PROTEIN US2M, MRPS2 × 1 （F1S001） MITORIBOSOMAL PROTEIN US3M, MRPS24 × 1 （F1ST79） MITORIBOSOMAL PROTEIN US5M, MRPS5 × 1 （F1SU66） MITORIBOSOMAL PROTEIN BS6M, MRPS6 × 1 MITORIBOSOMAL PROTEIN US7M, MRPS7 × 1 （F1RVX4） MITORIBOSOMAL PROTEIN US9M, MRPS9 × 1 MITORIBOSOMAL PROTEIN US10M, MRPS10 × 1 MITORIBOSOMAL PROTEIN US11M, MRPS11 × 1 （F1SRT1） MITORIBOSOMAL PROTEIN US12M, MRPS12 × 1 MITORIBOSOMAL PROTEIN US14M, MRPS14 × 1 MITORIBOSOMAL PROTEIN US15M, MRPS15 × 1 MITORIBOSOMAL PROTEIN BS16M, MRPS16 × 1 （F1SU70） MITORIBOSOMAL PROTEIN US17M, MRPS17 × 1 （F1RIU0） MITORIBOSOMAL PROTEIN BS18M, MRPS18C × 1 MITORIBOSOMAL PROTEIN BL19M, MRPL19 × 1 MITORIBOSOMAL PROTEIN BS21M, MRPS21 × 1 （I3L971） P-SITE AND A-SITE TRNA × 2 MRNA × 1 MITORIBOSOMAL PROTEIN MS22, MRPS22 × 1 MITORIBOSOMAL PROTEIN MS23, MRPS23 × 1 MITORIBOSOMAL PROTEIN MS25, MRPS25 × 1 MITORIBOSOMAL PROTEIN MS26, MRPS26 × 1 （F1S8A5） MITORIBOSOMAL PROTEIN MS27, MRPS27 × 1 MITORIBOSOMAL PROTEIN MS28, MRPS28 × 1 MITORIBOSOMAL PROTEIN MS29, MRPS29 × 1 MITORIBOSOMAL PROTEIN MS33, MRPS33 × 1 （F1S8G3） MITORIBOSOMAL PROTEIN MS34, MRPS34 × 1 MITORIBOSOMAL PROTEIN MS35, MRPS35 × 1 （F1SG95） MITORIBOSOMAL PROTEIN MS37, MRPS37 × 1 （F1SU49） MITORIBOSOMAL PROTEIN MS38, MRPS38 × 1 MITORIBOSOMAL PROTEIN MS39, MRPS39 × 1 （K7GKS8） MITORIBOSOMAL PROTEIN MS40, MRPS18B × 1 （Q767K8） UNASSIGNED HELICES × 1 UNASSIGNED HELICES × 1 MG MAGNESIUM ION × 144 ZN ZINC ION × 3 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY cryo-EM buffer：20 MM HEPES-KOH, 50 MM KCL, 40 MM MGCL2, 1 MM DTT；pH 7.4；20 MM HEPES-KOH, 50 MM KCL, 40 MM MGCL2, 1 MM DTT cryo-EM vitrification conditions：Cryogen ETHANE-PROPANE；MIXTURE OF LIQUID ETHANE AND PROPANE Resolution 3.60 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 5AJ3 Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
5AJ4 Structure of the 55S mammalian mitoribosome. Deposited 2015-02-20 Different construct Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 83 PDB declaration: 89-meric
Chain Ah 285–387（103 aa）
Not recorded MG MAGNESIUM ION × 351 ZN ZINC ION × 6 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer 20 MM HEPES-KOH, 50 MM KCL, 40 MM MGCL2, 1 MM DTT；pH 7.4；20 MM HEPES-KOH, 50 MM KCL, 40 MM MGCL2, 1 MM DTT
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；MIXTURE OF LIQUID ETHANE AND PROPANE
Resolution 3.80 Å
6YDP 55S mammalian mitochondrial ribosome with mtEFG1 and P site fMet-tRNAMet (POST) Deposited 2020-03-20 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 89 PDB declaration: 94-meric
Chain Ah 1–381（381 aa）
Not recorded MG MAGNESIUM ION × 326 ZN ZINC ION × 6 SPM SPERMINE × 3 FME N-FORMYLMETHIONINE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 5GP GUANOSINE-5'-MONOPHOSPHATE × 2 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.00 Å
6YDW 55S mammalian mitochondrial ribosome with mtEFG1 and two tRNAMet (TI-POST) Deposited 2020-03-21 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 89 PDB declaration: 95-meric
Chain Ah 1–381（381 aa）
Not recorded MG MAGNESIUM ION × 325 ZN ZINC ION × 6 SPM SPERMINE × 3 FME N-FORMYLMETHIONINE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 5GP GUANOSINE-5'-MONOPHOSPHATE × 1 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 4.20 Å
7NQH 55S mammalian mitochondrial ribosome with mtRF1a and P-site tRNAMet Deposited 2021-03-01 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 88 PDB declaration: 93-meric
Chain Ah 1–381（381 aa）
Not recorded MG MAGNESIUM ION × 323 ZN ZINC ION × 6 SPM SPERMINE × 3 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 5GP GUANOSINE-5'-MONOPHOSPHATE × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.50 Å
7NQL 55S mammalian mitochondrial ribosome with ICT1 and P site tRNAMet Deposited 2021-03-01 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 88 PDB declaration: 93-meric
Chain Ah 1–381（381 aa）
Not recorded ZN ZINC ION × 6 MG MAGNESIUM ION × 335 5GP GUANOSINE-5'-MONOPHOSPHATE × 2 SPM SPERMINE × 3 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.40 Å
8OIN 55S mammalian mitochondrial ribosome with mtRF1 and P-site tRNA Deposited 2023-03-23 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 88 PDB declaration: 94-meric
Chain AY 1–381（381 aa）
Not recorded MG MAGNESIUM ION × 332 K POTASSIUM ION × 41 PHE PHENYLALANINE × 1 ZN ZINC ION × 3 FES FE2/S2 (INORGANIC) CLUSTER × 3 SPM SPERMINE × 1 SPD SPERMIDINE × 1 FME N-FORMYLMETHIONINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20mM HEPES-KOH pH=7.5, 100mM KCl, 20mM MgCl2, ~1% sucrose (from mtRF1 storage buffer)
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.60 Å
8OIP 28S mammalian mitochondrial small ribosomal subunit with mtRF1 and P-site tRNA Deposited 2023-03-23 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 33 PDB declaration: 36-meric
Chain AY 1–381（381 aa）
Not recorded MG MAGNESIUM ION × 127 SPM SPERMINE × 1 SPD SPERMIDINE × 1 K POTASSIUM ION × 11 ZN ZINC ION × 1 FS2 FE-S-O HYBRID CLUSTER × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20mM HEPES-KOH pH=7.5, 100mM KCl, 20mM MgCl2, ~1% sucrose (from mtRF1 storage buffer)
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.60 Å

7 other PDB entries and 7 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name F1RME2_PIG
Isoform —
PDB entities 27
Chains and sequence ranges Author chain h； PDBConstruct 1–387； UniProt 1–387

MITORIBOSOMAL PROTEIN MS33, MRPS33

OrganismNot specified

UniProt F1S8G3

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein–RNA Heteromer Protein × 33 RNA 4 PDB declaration: 37-meric（37） Consistent with all polymer counts Chain i； UniProt 1–106 Not recorded MITORIBOSOMAL 12S RRNA × 1 MITORIBOSOMAL PROTEIN US2M, MRPS2 × 1 （F1S001） MITORIBOSOMAL PROTEIN US3M, MRPS24 × 1 （F1ST79） MITORIBOSOMAL PROTEIN US5M, MRPS5 × 1 （F1SU66） MITORIBOSOMAL PROTEIN BS6M, MRPS6 × 1 MITORIBOSOMAL PROTEIN US7M, MRPS7 × 1 （F1RVX4） MITORIBOSOMAL PROTEIN US9M, MRPS9 × 1 MITORIBOSOMAL PROTEIN US10M, MRPS10 × 1 MITORIBOSOMAL PROTEIN US11M, MRPS11 × 1 （F1SRT1） MITORIBOSOMAL PROTEIN US12M, MRPS12 × 1 MITORIBOSOMAL PROTEIN US14M, MRPS14 × 1 MITORIBOSOMAL PROTEIN US15M, MRPS15 × 1 MITORIBOSOMAL PROTEIN BS16M, MRPS16 × 1 （F1SU70） MITORIBOSOMAL PROTEIN US17M, MRPS17 × 1 （F1RIU0） MITORIBOSOMAL PROTEIN BS18M, MRPS18C × 1 MITORIBOSOMAL PROTEIN BL19M, MRPL19 × 1 MITORIBOSOMAL PROTEIN BS21M, MRPS21 × 1 （I3L971） P-SITE AND A-SITE TRNA × 2 MRNA × 1 MITORIBOSOMAL PROTEIN MS22, MRPS22 × 1 MITORIBOSOMAL PROTEIN MS23, MRPS23 × 1 MITORIBOSOMAL PROTEIN MS25, MRPS25 × 1 MITORIBOSOMAL PROTEIN MS26, MRPS26 × 1 （F1S8A5） MITORIBOSOMAL PROTEIN MS27, MRPS27 × 1 MITORIBOSOMAL PROTEIN MS28, MRPS28 × 1 MITORIBOSOMAL PROTEIN MS29, MRPS29 × 1 MITORIBOSOMAL PROTEIN MS31, MRPS31 × 1 （F1RME2） MITORIBOSOMAL PROTEIN MS34, MRPS34 × 1 MITORIBOSOMAL PROTEIN MS35, MRPS35 × 1 （F1SG95） MITORIBOSOMAL PROTEIN MS37, MRPS37 × 1 （F1SU49） MITORIBOSOMAL PROTEIN MS38, MRPS38 × 1 MITORIBOSOMAL PROTEIN MS39, MRPS39 × 1 （K7GKS8） MITORIBOSOMAL PROTEIN MS40, MRPS18B × 1 （Q767K8） UNASSIGNED HELICES × 1 UNASSIGNED HELICES × 1 MG MAGNESIUM ION × 144 ZN ZINC ION × 3 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY cryo-EM buffer：20 MM HEPES-KOH, 50 MM KCL, 40 MM MGCL2, 1 MM DTT；pH 7.4；20 MM HEPES-KOH, 50 MM KCL, 40 MM MGCL2, 1 MM DTT cryo-EM vitrification conditions：Cryogen ETHANE-PROPANE；MIXTURE OF LIQUID ETHANE AND PROPANE Resolution 3.60 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 5AJ3 Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
5AJ4 Structure of the 55S mammalian mitoribosome. Deposited 2015-02-20 Different construct Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 83 PDB declaration: 89-meric
Chain Ai 4–102（99 aa）
Not recorded MG MAGNESIUM ION × 351 ZN ZINC ION × 6 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer 20 MM HEPES-KOH, 50 MM KCL, 40 MM MGCL2, 1 MM DTT；pH 7.4；20 MM HEPES-KOH, 50 MM KCL, 40 MM MGCL2, 1 MM DTT
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；MIXTURE OF LIQUID ETHANE AND PROPANE
Resolution 3.80 Å
6GAW Unique features of mammalian mitochondrial translation initiation revealed by cryo-EM. This file contains the complete 55S ribosome. Deposited 2018-04-13 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 90 PDB declaration: 95-meric
Chain Ai 43–148（106 aa）
Not recorded MG MAGNESIUM ION × 326 ZN ZINC ION × 6 5GP GUANOSINE-5'-MONOPHOSPHATE × 2 SPM SPERMINE × 3 GSP 5'-GUANOSINE-DIPHOSPHATE-MONOTHIOPHOSPHATE × 1 NA SODIUM ION × 1 FME N-FORMYLMETHIONINE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
6GAZ Unique features of mammalian mitochondrial translation initiation revealed by cryo-EM. This file contains the 28S ribosomal subunit. Deposited 2018-04-13 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 33 PDB declaration: 36-meric
Chain Ai 43–148（106 aa）
Not recorded GSP 5'-GUANOSINE-DIPHOSPHATE-MONOTHIOPHOSPHATE × 1 MG MAGNESIUM ION × 111 NA SODIUM ION × 1 SPM SPERMINE × 1 ZN ZINC ION × 3 FME N-FORMYLMETHIONINE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
6YDP 55S mammalian mitochondrial ribosome with mtEFG1 and P site fMet-tRNAMet (POST) Deposited 2020-03-20 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 89 PDB declaration: 94-meric
Chain Ai 43–148（106 aa）
Not recorded MG MAGNESIUM ION × 326 ZN ZINC ION × 6 SPM SPERMINE × 3 FME N-FORMYLMETHIONINE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 5GP GUANOSINE-5'-MONOPHOSPHATE × 2 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.00 Å
6YDW 55S mammalian mitochondrial ribosome with mtEFG1 and two tRNAMet (TI-POST) Deposited 2020-03-21 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 89 PDB declaration: 95-meric
Chain Ai 43–148（106 aa）
Not recorded MG MAGNESIUM ION × 325 ZN ZINC ION × 6 SPM SPERMINE × 3 FME N-FORMYLMETHIONINE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 5GP GUANOSINE-5'-MONOPHOSPHATE × 1 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 4.20 Å
7NQH 55S mammalian mitochondrial ribosome with mtRF1a and P-site tRNAMet Deposited 2021-03-01 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 88 PDB declaration: 93-meric
Chain Ai 43–148（106 aa）
Not recorded MG MAGNESIUM ION × 323 ZN ZINC ION × 6 SPM SPERMINE × 3 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 5GP GUANOSINE-5'-MONOPHOSPHATE × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.50 Å
7NQL 55S mammalian mitochondrial ribosome with ICT1 and P site tRNAMet Deposited 2021-03-01 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 88 PDB declaration: 93-meric
Chain Ai 43–148（106 aa）
Not recorded ZN ZINC ION × 6 MG MAGNESIUM ION × 335 5GP GUANOSINE-5'-MONOPHOSPHATE × 2 SPM SPERMINE × 3 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.40 Å
7NSI 55S mammalian mitochondrial ribosome with mtRRF (pre) and tRNA(P/E) Deposited 2021-03-07 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 88 PDB declaration: 93-meric
Chain Ai 43–148（106 aa）
Not recorded MG MAGNESIUM ION × 327 ZN ZINC ION × 6 SPM SPERMINE × 3 5GP GUANOSINE-5'-MONOPHOSPHATE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 4.60 Å
7NSJ 55S mammalian mitochondrial ribosome with tRNA(P/P) and tRNA(E*) Deposited 2021-03-07 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 87 PDB declaration: 93-meric
Chain Ai 43–148（106 aa）
Not recorded MG MAGNESIUM ION × 324 ZN ZINC ION × 6 SPM SPERMINE × 3 5GP GUANOSINE-5'-MONOPHOSPHATE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.90 Å
8OIN 55S mammalian mitochondrial ribosome with mtRF1 and P-site tRNA Deposited 2023-03-23 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 88 PDB declaration: 94-meric
Chain AZ 1–148（148 aa）
Not recorded MG MAGNESIUM ION × 332 K POTASSIUM ION × 41 PHE PHENYLALANINE × 1 ZN ZINC ION × 3 FES FE2/S2 (INORGANIC) CLUSTER × 3 SPM SPERMINE × 1 SPD SPERMIDINE × 1 FME N-FORMYLMETHIONINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20mM HEPES-KOH pH=7.5, 100mM KCl, 20mM MgCl2, ~1% sucrose (from mtRF1 storage buffer)
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.60 Å
8OIP 28S mammalian mitochondrial small ribosomal subunit with mtRF1 and P-site tRNA Deposited 2023-03-23 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 33 PDB declaration: 36-meric
Chain AZ 1–148（148 aa）
Not recorded MG MAGNESIUM ION × 127 SPM SPERMINE × 1 SPD SPERMIDINE × 1 K POTASSIUM ION × 11 ZN ZINC ION × 1 FS2 FE-S-O HYBRID CLUSTER × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20mM HEPES-KOH pH=7.5, 100mM KCl, 20mM MgCl2, ~1% sucrose (from mtRF1 storage buffer)
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.60 Å

11 other PDB entries and 11 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name F1S8G3_PIG
Isoform —
PDB entities 28
Chains and sequence ranges Author chain i； PDBConstruct 1–106； UniProt 1–106

MITORIBOSOMAL PROTEIN MS35, MRPS35

OrganismNot specified

UniProt F1SG95

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein–RNA Heteromer Protein × 33 RNA 4 PDB declaration: 37-meric（37） Consistent with all polymer counts Chain k； UniProt 1–325 Not recorded MITORIBOSOMAL 12S RRNA × 1 MITORIBOSOMAL PROTEIN US2M, MRPS2 × 1 （F1S001） MITORIBOSOMAL PROTEIN US3M, MRPS24 × 1 （F1ST79） MITORIBOSOMAL PROTEIN US5M, MRPS5 × 1 （F1SU66） MITORIBOSOMAL PROTEIN BS6M, MRPS6 × 1 MITORIBOSOMAL PROTEIN US7M, MRPS7 × 1 （F1RVX4） MITORIBOSOMAL PROTEIN US9M, MRPS9 × 1 MITORIBOSOMAL PROTEIN US10M, MRPS10 × 1 MITORIBOSOMAL PROTEIN US11M, MRPS11 × 1 （F1SRT1） MITORIBOSOMAL PROTEIN US12M, MRPS12 × 1 MITORIBOSOMAL PROTEIN US14M, MRPS14 × 1 MITORIBOSOMAL PROTEIN US15M, MRPS15 × 1 MITORIBOSOMAL PROTEIN BS16M, MRPS16 × 1 （F1SU70） MITORIBOSOMAL PROTEIN US17M, MRPS17 × 1 （F1RIU0） MITORIBOSOMAL PROTEIN BS18M, MRPS18C × 1 MITORIBOSOMAL PROTEIN BL19M, MRPL19 × 1 MITORIBOSOMAL PROTEIN BS21M, MRPS21 × 1 （I3L971） P-SITE AND A-SITE TRNA × 2 MRNA × 1 MITORIBOSOMAL PROTEIN MS22, MRPS22 × 1 MITORIBOSOMAL PROTEIN MS23, MRPS23 × 1 MITORIBOSOMAL PROTEIN MS25, MRPS25 × 1 MITORIBOSOMAL PROTEIN MS26, MRPS26 × 1 （F1S8A5） MITORIBOSOMAL PROTEIN MS27, MRPS27 × 1 MITORIBOSOMAL PROTEIN MS28, MRPS28 × 1 MITORIBOSOMAL PROTEIN MS29, MRPS29 × 1 MITORIBOSOMAL PROTEIN MS31, MRPS31 × 1 （F1RME2） MITORIBOSOMAL PROTEIN MS33, MRPS33 × 1 （F1S8G3） MITORIBOSOMAL PROTEIN MS34, MRPS34 × 1 MITORIBOSOMAL PROTEIN MS37, MRPS37 × 1 （F1SU49） MITORIBOSOMAL PROTEIN MS38, MRPS38 × 1 MITORIBOSOMAL PROTEIN MS39, MRPS39 × 1 （K7GKS8） MITORIBOSOMAL PROTEIN MS40, MRPS18B × 1 （Q767K8） UNASSIGNED HELICES × 1 UNASSIGNED HELICES × 1 MG MAGNESIUM ION × 144 ZN ZINC ION × 3 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY cryo-EM buffer：20 MM HEPES-KOH, 50 MM KCL, 40 MM MGCL2, 1 MM DTT；pH 7.4；20 MM HEPES-KOH, 50 MM KCL, 40 MM MGCL2, 1 MM DTT cryo-EM vitrification conditions：Cryogen ETHANE-PROPANE；MIXTURE OF LIQUID ETHANE AND PROPANE Resolution 3.60 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 5AJ3 Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
5AJ4 Structure of the 55S mammalian mitoribosome. Deposited 2015-02-20 Different construct Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 83 PDB declaration: 89-meric
Chain Ak 51–325（275 aa）
Not recorded MG MAGNESIUM ION × 351 ZN ZINC ION × 6 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer 20 MM HEPES-KOH, 50 MM KCL, 40 MM MGCL2, 1 MM DTT；pH 7.4；20 MM HEPES-KOH, 50 MM KCL, 40 MM MGCL2, 1 MM DTT
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；MIXTURE OF LIQUID ETHANE AND PROPANE
Resolution 3.80 Å
6GAW Unique features of mammalian mitochondrial translation initiation revealed by cryo-EM. This file contains the complete 55S ribosome. Deposited 2018-04-13 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 90 PDB declaration: 95-meric
Chain Ak 42–366（325 aa）
Not recorded MG MAGNESIUM ION × 326 ZN ZINC ION × 6 5GP GUANOSINE-5'-MONOPHOSPHATE × 2 SPM SPERMINE × 3 GSP 5'-GUANOSINE-DIPHOSPHATE-MONOTHIOPHOSPHATE × 1 NA SODIUM ION × 1 FME N-FORMYLMETHIONINE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
6GAZ Unique features of mammalian mitochondrial translation initiation revealed by cryo-EM. This file contains the 28S ribosomal subunit. Deposited 2018-04-13 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 33 PDB declaration: 36-meric
Chain Ak 42–366（325 aa）
Not recorded GSP 5'-GUANOSINE-DIPHOSPHATE-MONOTHIOPHOSPHATE × 1 MG MAGNESIUM ION × 111 NA SODIUM ION × 1 SPM SPERMINE × 1 ZN ZINC ION × 3 FME N-FORMYLMETHIONINE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
6YDP 55S mammalian mitochondrial ribosome with mtEFG1 and P site fMet-tRNAMet (POST) Deposited 2020-03-20 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 89 PDB declaration: 94-meric
Chain Ak 42–366（325 aa）
Not recorded MG MAGNESIUM ION × 326 ZN ZINC ION × 6 SPM SPERMINE × 3 FME N-FORMYLMETHIONINE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 5GP GUANOSINE-5'-MONOPHOSPHATE × 2 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.00 Å
6YDW 55S mammalian mitochondrial ribosome with mtEFG1 and two tRNAMet (TI-POST) Deposited 2020-03-21 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 89 PDB declaration: 95-meric
Chain Ak 42–366（325 aa）
Not recorded MG MAGNESIUM ION × 325 ZN ZINC ION × 6 SPM SPERMINE × 3 FME N-FORMYLMETHIONINE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 5GP GUANOSINE-5'-MONOPHOSPHATE × 1 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 4.20 Å
7NQH 55S mammalian mitochondrial ribosome with mtRF1a and P-site tRNAMet Deposited 2021-03-01 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 88 PDB declaration: 93-meric
Chain Ak 42–366（325 aa）
Not recorded MG MAGNESIUM ION × 323 ZN ZINC ION × 6 SPM SPERMINE × 3 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 5GP GUANOSINE-5'-MONOPHOSPHATE × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.50 Å
7NQL 55S mammalian mitochondrial ribosome with ICT1 and P site tRNAMet Deposited 2021-03-01 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 88 PDB declaration: 93-meric
Chain Ak 42–366（325 aa）
Not recorded ZN ZINC ION × 6 MG MAGNESIUM ION × 335 5GP GUANOSINE-5'-MONOPHOSPHATE × 2 SPM SPERMINE × 3 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.40 Å
7NSI 55S mammalian mitochondrial ribosome with mtRRF (pre) and tRNA(P/E) Deposited 2021-03-07 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 88 PDB declaration: 93-meric
Chain Ak 42–366（325 aa）
Not recorded MG MAGNESIUM ION × 327 ZN ZINC ION × 6 SPM SPERMINE × 3 5GP GUANOSINE-5'-MONOPHOSPHATE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 4.60 Å
7NSJ 55S mammalian mitochondrial ribosome with tRNA(P/P) and tRNA(E*) Deposited 2021-03-07 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 87 PDB declaration: 93-meric
Chain Ak 42–366（325 aa）
Not recorded MG MAGNESIUM ION × 324 ZN ZINC ION × 6 SPM SPERMINE × 3 5GP GUANOSINE-5'-MONOPHOSPHATE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.90 Å
8OIN 55S mammalian mitochondrial ribosome with mtRF1 and P-site tRNA Deposited 2023-03-23 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 88 PDB declaration: 94-meric
Chain AB 1–366（366 aa）
Not recorded MG MAGNESIUM ION × 332 K POTASSIUM ION × 41 PHE PHENYLALANINE × 1 ZN ZINC ION × 3 FES FE2/S2 (INORGANIC) CLUSTER × 3 SPM SPERMINE × 1 SPD SPERMIDINE × 1 FME N-FORMYLMETHIONINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20mM HEPES-KOH pH=7.5, 100mM KCl, 20mM MgCl2, ~1% sucrose (from mtRF1 storage buffer)
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.60 Å
8OIP 28S mammalian mitochondrial small ribosomal subunit with mtRF1 and P-site tRNA Deposited 2023-03-23 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 33 PDB declaration: 36-meric
Chain AB 1–366（366 aa）
Not recorded MG MAGNESIUM ION × 127 SPM SPERMINE × 1 SPD SPERMIDINE × 1 K POTASSIUM ION × 11 ZN ZINC ION × 1 FS2 FE-S-O HYBRID CLUSTER × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20mM HEPES-KOH pH=7.5, 100mM KCl, 20mM MgCl2, ~1% sucrose (from mtRF1 storage buffer)
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.60 Å

11 other PDB entries and 11 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name F1SG95_PIG
Isoform —
PDB entities 30
Chains and sequence ranges Author chain k； PDBConstruct 1–325； UniProt 1–325

MITORIBOSOMAL PROTEIN MS37, MRPS37

OrganismNot specified

UniProt F1SU49

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein–RNA Heteromer Protein × 33 RNA 4 PDB declaration: 37-meric（37） Consistent with all polymer counts Chain m； UniProt 1–118 Not recorded MITORIBOSOMAL 12S RRNA × 1 MITORIBOSOMAL PROTEIN US2M, MRPS2 × 1 （F1S001） MITORIBOSOMAL PROTEIN US3M, MRPS24 × 1 （F1ST79） MITORIBOSOMAL PROTEIN US5M, MRPS5 × 1 （F1SU66） MITORIBOSOMAL PROTEIN BS6M, MRPS6 × 1 MITORIBOSOMAL PROTEIN US7M, MRPS7 × 1 （F1RVX4） MITORIBOSOMAL PROTEIN US9M, MRPS9 × 1 MITORIBOSOMAL PROTEIN US10M, MRPS10 × 1 MITORIBOSOMAL PROTEIN US11M, MRPS11 × 1 （F1SRT1） MITORIBOSOMAL PROTEIN US12M, MRPS12 × 1 MITORIBOSOMAL PROTEIN US14M, MRPS14 × 1 MITORIBOSOMAL PROTEIN US15M, MRPS15 × 1 MITORIBOSOMAL PROTEIN BS16M, MRPS16 × 1 （F1SU70） MITORIBOSOMAL PROTEIN US17M, MRPS17 × 1 （F1RIU0） MITORIBOSOMAL PROTEIN BS18M, MRPS18C × 1 MITORIBOSOMAL PROTEIN BL19M, MRPL19 × 1 MITORIBOSOMAL PROTEIN BS21M, MRPS21 × 1 （I3L971） P-SITE AND A-SITE TRNA × 2 MRNA × 1 MITORIBOSOMAL PROTEIN MS22, MRPS22 × 1 MITORIBOSOMAL PROTEIN MS23, MRPS23 × 1 MITORIBOSOMAL PROTEIN MS25, MRPS25 × 1 MITORIBOSOMAL PROTEIN MS26, MRPS26 × 1 （F1S8A5） MITORIBOSOMAL PROTEIN MS27, MRPS27 × 1 MITORIBOSOMAL PROTEIN MS28, MRPS28 × 1 MITORIBOSOMAL PROTEIN MS29, MRPS29 × 1 MITORIBOSOMAL PROTEIN MS31, MRPS31 × 1 （F1RME2） MITORIBOSOMAL PROTEIN MS33, MRPS33 × 1 （F1S8G3） MITORIBOSOMAL PROTEIN MS34, MRPS34 × 1 MITORIBOSOMAL PROTEIN MS35, MRPS35 × 1 （F1SG95） MITORIBOSOMAL PROTEIN MS38, MRPS38 × 1 MITORIBOSOMAL PROTEIN MS39, MRPS39 × 1 （K7GKS8） MITORIBOSOMAL PROTEIN MS40, MRPS18B × 1 （Q767K8） UNASSIGNED HELICES × 1 UNASSIGNED HELICES × 1 MG MAGNESIUM ION × 144 ZN ZINC ION × 3 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY cryo-EM buffer：20 MM HEPES-KOH, 50 MM KCL, 40 MM MGCL2, 1 MM DTT；pH 7.4；20 MM HEPES-KOH, 50 MM KCL, 40 MM MGCL2, 1 MM DTT cryo-EM vitrification conditions：Cryogen ETHANE-PROPANE；MIXTURE OF LIQUID ETHANE AND PROPANE Resolution 3.60 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 5AJ3 Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
5AJ4 Structure of the 55S mammalian mitoribosome. Deposited 2015-02-20 Different construct Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 83 PDB declaration: 89-meric
Chain Am 2–117（116 aa）
Not recorded MG MAGNESIUM ION × 351 ZN ZINC ION × 6 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer 20 MM HEPES-KOH, 50 MM KCL, 40 MM MGCL2, 1 MM DTT；pH 7.4；20 MM HEPES-KOH, 50 MM KCL, 40 MM MGCL2, 1 MM DTT
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；MIXTURE OF LIQUID ETHANE AND PROPANE
Resolution 3.80 Å
6GAW Unique features of mammalian mitochondrial translation initiation revealed by cryo-EM. This file contains the complete 55S ribosome. Deposited 2018-04-13 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 90 PDB declaration: 95-meric
Chain Am 1–118（118 aa）
Not recorded MG MAGNESIUM ION × 326 ZN ZINC ION × 6 5GP GUANOSINE-5'-MONOPHOSPHATE × 2 SPM SPERMINE × 3 GSP 5'-GUANOSINE-DIPHOSPHATE-MONOTHIOPHOSPHATE × 1 NA SODIUM ION × 1 FME N-FORMYLMETHIONINE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
6GAZ Unique features of mammalian mitochondrial translation initiation revealed by cryo-EM. This file contains the 28S ribosomal subunit. Deposited 2018-04-13 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 33 PDB declaration: 36-meric
Chain Am 1–118（118 aa）
Not recorded GSP 5'-GUANOSINE-DIPHOSPHATE-MONOTHIOPHOSPHATE × 1 MG MAGNESIUM ION × 111 NA SODIUM ION × 1 SPM SPERMINE × 1 ZN ZINC ION × 3 FME N-FORMYLMETHIONINE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
6YDP 55S mammalian mitochondrial ribosome with mtEFG1 and P site fMet-tRNAMet (POST) Deposited 2020-03-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 89 PDB declaration: 94-meric
Chain Am 1–118（118 aa）
Not recorded MG MAGNESIUM ION × 326 ZN ZINC ION × 6 SPM SPERMINE × 3 FME N-FORMYLMETHIONINE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 5GP GUANOSINE-5'-MONOPHOSPHATE × 2 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.00 Å
6YDW 55S mammalian mitochondrial ribosome with mtEFG1 and two tRNAMet (TI-POST) Deposited 2020-03-21 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 89 PDB declaration: 95-meric
Chain Am 1–118（118 aa）
Not recorded MG MAGNESIUM ION × 325 ZN ZINC ION × 6 SPM SPERMINE × 3 FME N-FORMYLMETHIONINE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 5GP GUANOSINE-5'-MONOPHOSPHATE × 1 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 4.20 Å
7NQH 55S mammalian mitochondrial ribosome with mtRF1a and P-site tRNAMet Deposited 2021-03-01 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 88 PDB declaration: 93-meric
Chain Am 1–118（118 aa）
Not recorded MG MAGNESIUM ION × 323 ZN ZINC ION × 6 SPM SPERMINE × 3 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 5GP GUANOSINE-5'-MONOPHOSPHATE × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.50 Å
7NQL 55S mammalian mitochondrial ribosome with ICT1 and P site tRNAMet Deposited 2021-03-01 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 88 PDB declaration: 93-meric
Chain Am 1–118（118 aa）
Not recorded ZN ZINC ION × 6 MG MAGNESIUM ION × 335 5GP GUANOSINE-5'-MONOPHOSPHATE × 2 SPM SPERMINE × 3 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.40 Å
7NSI 55S mammalian mitochondrial ribosome with mtRRF (pre) and tRNA(P/E) Deposited 2021-03-07 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 88 PDB declaration: 93-meric
Chain Am 1–118（118 aa）
Not recorded MG MAGNESIUM ION × 327 ZN ZINC ION × 6 SPM SPERMINE × 3 5GP GUANOSINE-5'-MONOPHOSPHATE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 4.60 Å
7NSJ 55S mammalian mitochondrial ribosome with tRNA(P/P) and tRNA(E*) Deposited 2021-03-07 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 87 PDB declaration: 93-meric
Chain Am 1–118（118 aa）
Not recorded MG MAGNESIUM ION × 324 ZN ZINC ION × 6 SPM SPERMINE × 3 5GP GUANOSINE-5'-MONOPHOSPHATE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.90 Å
8OIN 55S mammalian mitochondrial ribosome with mtRF1 and P-site tRNA Deposited 2023-03-23 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 88 PDB declaration: 94-meric
Chain Ac 1–118（118 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) MG MAGNESIUM ION × 332 K POTASSIUM ION × 41 PHE PHENYLALANINE × 1 ZN ZINC ION × 3 FES FE2/S2 (INORGANIC) CLUSTER × 3 SPM SPERMINE × 1 SPD SPERMIDINE × 1 FME N-FORMYLMETHIONINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20mM HEPES-KOH pH=7.5, 100mM KCl, 20mM MgCl2, ~1% sucrose (from mtRF1 storage buffer)
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.60 Å
8OIP 28S mammalian mitochondrial small ribosomal subunit with mtRF1 and P-site tRNA Deposited 2023-03-23 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 33 PDB declaration: 36-meric
Chain Ac 1–118（118 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) MG MAGNESIUM ION × 127 SPM SPERMINE × 1 SPD SPERMIDINE × 1 K POTASSIUM ION × 11 ZN ZINC ION × 1 FS2 FE-S-O HYBRID CLUSTER × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20mM HEPES-KOH pH=7.5, 100mM KCl, 20mM MgCl2, ~1% sucrose (from mtRF1 storage buffer)
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.60 Å

11 other PDB entries and 11 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name F1SU49_PIG
Isoform —
PDB entities 31
Chains and sequence ranges Author chain m； PDBConstruct 1–118； UniProt 1–118

MITORIBOSOMAL PROTEIN MS39, MRPS39

OrganismNot specified

UniProt K7GKS8

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein–RNA Heteromer Protein × 33 RNA 4 PDB declaration: 37-meric（37） Consistent with all polymer counts Chain o； UniProt 1–692 Not recorded MITORIBOSOMAL 12S RRNA × 1 MITORIBOSOMAL PROTEIN US2M, MRPS2 × 1 （F1S001） MITORIBOSOMAL PROTEIN US3M, MRPS24 × 1 （F1ST79） MITORIBOSOMAL PROTEIN US5M, MRPS5 × 1 （F1SU66） MITORIBOSOMAL PROTEIN BS6M, MRPS6 × 1 MITORIBOSOMAL PROTEIN US7M, MRPS7 × 1 （F1RVX4） MITORIBOSOMAL PROTEIN US9M, MRPS9 × 1 MITORIBOSOMAL PROTEIN US10M, MRPS10 × 1 MITORIBOSOMAL PROTEIN US11M, MRPS11 × 1 （F1SRT1） MITORIBOSOMAL PROTEIN US12M, MRPS12 × 1 MITORIBOSOMAL PROTEIN US14M, MRPS14 × 1 MITORIBOSOMAL PROTEIN US15M, MRPS15 × 1 MITORIBOSOMAL PROTEIN BS16M, MRPS16 × 1 （F1SU70） MITORIBOSOMAL PROTEIN US17M, MRPS17 × 1 （F1RIU0） MITORIBOSOMAL PROTEIN BS18M, MRPS18C × 1 MITORIBOSOMAL PROTEIN BL19M, MRPL19 × 1 MITORIBOSOMAL PROTEIN BS21M, MRPS21 × 1 （I3L971） P-SITE AND A-SITE TRNA × 2 MRNA × 1 MITORIBOSOMAL PROTEIN MS22, MRPS22 × 1 MITORIBOSOMAL PROTEIN MS23, MRPS23 × 1 MITORIBOSOMAL PROTEIN MS25, MRPS25 × 1 MITORIBOSOMAL PROTEIN MS26, MRPS26 × 1 （F1S8A5） MITORIBOSOMAL PROTEIN MS27, MRPS27 × 1 MITORIBOSOMAL PROTEIN MS28, MRPS28 × 1 MITORIBOSOMAL PROTEIN MS29, MRPS29 × 1 MITORIBOSOMAL PROTEIN MS31, MRPS31 × 1 （F1RME2） MITORIBOSOMAL PROTEIN MS33, MRPS33 × 1 （F1S8G3） MITORIBOSOMAL PROTEIN MS34, MRPS34 × 1 MITORIBOSOMAL PROTEIN MS35, MRPS35 × 1 （F1SG95） MITORIBOSOMAL PROTEIN MS37, MRPS37 × 1 （F1SU49） MITORIBOSOMAL PROTEIN MS38, MRPS38 × 1 MITORIBOSOMAL PROTEIN MS40, MRPS18B × 1 （Q767K8） UNASSIGNED HELICES × 1 UNASSIGNED HELICES × 1 MG MAGNESIUM ION × 144 ZN ZINC ION × 3 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY cryo-EM buffer：20 MM HEPES-KOH, 50 MM KCL, 40 MM MGCL2, 1 MM DTT；pH 7.4；20 MM HEPES-KOH, 50 MM KCL, 40 MM MGCL2, 1 MM DTT cryo-EM vitrification conditions：Cryogen ETHANE-PROPANE；MIXTURE OF LIQUID ETHANE AND PROPANE Resolution 3.60 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 5AJ3 Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
5AJ4 Structure of the 55S mammalian mitoribosome. Deposited 2015-02-20 Different construct Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 83 PDB declaration: 89-meric
Chain Ao 1–143（143 aa）
Not recorded MG MAGNESIUM ION × 351 ZN ZINC ION × 6 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer 20 MM HEPES-KOH, 50 MM KCL, 40 MM MGCL2, 1 MM DTT；pH 7.4；20 MM HEPES-KOH, 50 MM KCL, 40 MM MGCL2, 1 MM DTT
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；MIXTURE OF LIQUID ETHANE AND PROPANE
Resolution 3.80 Å
6GAW Unique features of mammalian mitochondrial translation initiation revealed by cryo-EM. This file contains the complete 55S ribosome. Deposited 2018-04-13 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 90 PDB declaration: 95-meric
Chain Ao 1–692（692 aa）
Not recorded MG MAGNESIUM ION × 326 ZN ZINC ION × 6 5GP GUANOSINE-5'-MONOPHOSPHATE × 2 SPM SPERMINE × 3 GSP 5'-GUANOSINE-DIPHOSPHATE-MONOTHIOPHOSPHATE × 1 NA SODIUM ION × 1 FME N-FORMYLMETHIONINE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
6GAZ Unique features of mammalian mitochondrial translation initiation revealed by cryo-EM. This file contains the 28S ribosomal subunit. Deposited 2018-04-13 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 33 PDB declaration: 36-meric
Chain Ao 1–692（692 aa）
Not recorded GSP 5'-GUANOSINE-DIPHOSPHATE-MONOTHIOPHOSPHATE × 1 MG MAGNESIUM ION × 111 NA SODIUM ION × 1 SPM SPERMINE × 1 ZN ZINC ION × 3 FME N-FORMYLMETHIONINE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
6YDP 55S mammalian mitochondrial ribosome with mtEFG1 and P site fMet-tRNAMet (POST) Deposited 2020-03-20 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 89 PDB declaration: 94-meric
Chain Ao 1–699（699 aa）
Not recorded MG MAGNESIUM ION × 326 ZN ZINC ION × 6 SPM SPERMINE × 3 FME N-FORMYLMETHIONINE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 5GP GUANOSINE-5'-MONOPHOSPHATE × 2 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.00 Å
6YDW 55S mammalian mitochondrial ribosome with mtEFG1 and two tRNAMet (TI-POST) Deposited 2020-03-21 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 89 PDB declaration: 95-meric
Chain Ao 1–692（692 aa）
Not recorded MG MAGNESIUM ION × 325 ZN ZINC ION × 6 SPM SPERMINE × 3 FME N-FORMYLMETHIONINE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 5GP GUANOSINE-5'-MONOPHOSPHATE × 1 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 4.20 Å
7NQH 55S mammalian mitochondrial ribosome with mtRF1a and P-site tRNAMet Deposited 2021-03-01 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 88 PDB declaration: 93-meric
Chain Ao 1–699（699 aa）
Not recorded MG MAGNESIUM ION × 323 ZN ZINC ION × 6 SPM SPERMINE × 3 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 5GP GUANOSINE-5'-MONOPHOSPHATE × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.50 Å
7NQL 55S mammalian mitochondrial ribosome with ICT1 and P site tRNAMet Deposited 2021-03-01 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 88 PDB declaration: 93-meric
Chain Ao 1–699（699 aa）
Not recorded ZN ZINC ION × 6 MG MAGNESIUM ION × 335 5GP GUANOSINE-5'-MONOPHOSPHATE × 2 SPM SPERMINE × 3 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.40 Å
8OIN 55S mammalian mitochondrial ribosome with mtRF1 and P-site tRNA Deposited 2023-03-23 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 88 PDB declaration: 94-meric
Chain Ae 1–692（692 aa）
Not recorded MG MAGNESIUM ION × 332 K POTASSIUM ION × 41 PHE PHENYLALANINE × 1 ZN ZINC ION × 3 FES FE2/S2 (INORGANIC) CLUSTER × 3 SPM SPERMINE × 1 SPD SPERMIDINE × 1 FME N-FORMYLMETHIONINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20mM HEPES-KOH pH=7.5, 100mM KCl, 20mM MgCl2, ~1% sucrose (from mtRF1 storage buffer)
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.60 Å
8OIP 28S mammalian mitochondrial small ribosomal subunit with mtRF1 and P-site tRNA Deposited 2023-03-23 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 33 PDB declaration: 36-meric
Chain Ae 1–692（692 aa）
Not recorded MG MAGNESIUM ION × 127 SPM SPERMINE × 1 SPD SPERMIDINE × 1 K POTASSIUM ION × 11 ZN ZINC ION × 1 FS2 FE-S-O HYBRID CLUSTER × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20mM HEPES-KOH pH=7.5, 100mM KCl, 20mM MgCl2, ~1% sucrose (from mtRF1 storage buffer)
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.60 Å

9 other PDB entries and 9 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name K7GKS8_PIG
Isoform —
PDB entities 33
Chains and sequence ranges Author chain o； PDBConstruct 1–692； UniProt 1–692

MITORIBOSOMAL PROTEIN MS40, MRPS18B

OrganismNot specified

UniProt Q767K8

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein–RNA Heteromer Protein × 33 RNA 4 PDB declaration: 37-meric（37） Consistent with all polymer counts Chain p； UniProt 1–258 Not recorded MITORIBOSOMAL 12S RRNA × 1 MITORIBOSOMAL PROTEIN US2M, MRPS2 × 1 （F1S001） MITORIBOSOMAL PROTEIN US3M, MRPS24 × 1 （F1ST79） MITORIBOSOMAL PROTEIN US5M, MRPS5 × 1 （F1SU66） MITORIBOSOMAL PROTEIN BS6M, MRPS6 × 1 MITORIBOSOMAL PROTEIN US7M, MRPS7 × 1 （F1RVX4） MITORIBOSOMAL PROTEIN US9M, MRPS9 × 1 MITORIBOSOMAL PROTEIN US10M, MRPS10 × 1 MITORIBOSOMAL PROTEIN US11M, MRPS11 × 1 （F1SRT1） MITORIBOSOMAL PROTEIN US12M, MRPS12 × 1 MITORIBOSOMAL PROTEIN US14M, MRPS14 × 1 MITORIBOSOMAL PROTEIN US15M, MRPS15 × 1 MITORIBOSOMAL PROTEIN BS16M, MRPS16 × 1 （F1SU70） MITORIBOSOMAL PROTEIN US17M, MRPS17 × 1 （F1RIU0） MITORIBOSOMAL PROTEIN BS18M, MRPS18C × 1 MITORIBOSOMAL PROTEIN BL19M, MRPL19 × 1 MITORIBOSOMAL PROTEIN BS21M, MRPS21 × 1 （I3L971） P-SITE AND A-SITE TRNA × 2 MRNA × 1 MITORIBOSOMAL PROTEIN MS22, MRPS22 × 1 MITORIBOSOMAL PROTEIN MS23, MRPS23 × 1 MITORIBOSOMAL PROTEIN MS25, MRPS25 × 1 MITORIBOSOMAL PROTEIN MS26, MRPS26 × 1 （F1S8A5） MITORIBOSOMAL PROTEIN MS27, MRPS27 × 1 MITORIBOSOMAL PROTEIN MS28, MRPS28 × 1 MITORIBOSOMAL PROTEIN MS29, MRPS29 × 1 MITORIBOSOMAL PROTEIN MS31, MRPS31 × 1 （F1RME2） MITORIBOSOMAL PROTEIN MS33, MRPS33 × 1 （F1S8G3） MITORIBOSOMAL PROTEIN MS34, MRPS34 × 1 MITORIBOSOMAL PROTEIN MS35, MRPS35 × 1 （F1SG95） MITORIBOSOMAL PROTEIN MS37, MRPS37 × 1 （F1SU49） MITORIBOSOMAL PROTEIN MS38, MRPS38 × 1 MITORIBOSOMAL PROTEIN MS39, MRPS39 × 1 （K7GKS8） UNASSIGNED HELICES × 1 UNASSIGNED HELICES × 1 MG MAGNESIUM ION × 144 ZN ZINC ION × 3 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY cryo-EM buffer：20 MM HEPES-KOH, 50 MM KCL, 40 MM MGCL2, 1 MM DTT；pH 7.4；20 MM HEPES-KOH, 50 MM KCL, 40 MM MGCL2, 1 MM DTT cryo-EM vitrification conditions：Cryogen ETHANE-PROPANE；MIXTURE OF LIQUID ETHANE AND PROPANE Resolution 3.60 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 5AJ3 Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
5AJ4 Structure of the 55S mammalian mitoribosome. Deposited 2015-02-20 Different construct Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 83 PDB declaration: 89-meric
Chain Ap 51–238（188 aa）
Not recorded MG MAGNESIUM ION × 351 ZN ZINC ION × 6 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer 20 MM HEPES-KOH, 50 MM KCL, 40 MM MGCL2, 1 MM DTT；pH 7.4；20 MM HEPES-KOH, 50 MM KCL, 40 MM MGCL2, 1 MM DTT
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；MIXTURE OF LIQUID ETHANE AND PROPANE
Resolution 3.80 Å
6GAW Unique features of mammalian mitochondrial translation initiation revealed by cryo-EM. This file contains the complete 55S ribosome. Deposited 2018-04-13 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 90 PDB declaration: 95-meric
Chain Ap 1–258（258 aa）
Not recorded MG MAGNESIUM ION × 326 ZN ZINC ION × 6 5GP GUANOSINE-5'-MONOPHOSPHATE × 2 SPM SPERMINE × 3 GSP 5'-GUANOSINE-DIPHOSPHATE-MONOTHIOPHOSPHATE × 1 NA SODIUM ION × 1 FME N-FORMYLMETHIONINE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
6GAZ Unique features of mammalian mitochondrial translation initiation revealed by cryo-EM. This file contains the 28S ribosomal subunit. Deposited 2018-04-13 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 33 PDB declaration: 36-meric
Chain Ap 1–258（258 aa）
Not recorded GSP 5'-GUANOSINE-DIPHOSPHATE-MONOTHIOPHOSPHATE × 1 MG MAGNESIUM ION × 111 NA SODIUM ION × 1 SPM SPERMINE × 1 ZN ZINC ION × 3 FME N-FORMYLMETHIONINE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
6YDP 55S mammalian mitochondrial ribosome with mtEFG1 and P site fMet-tRNAMet (POST) Deposited 2020-03-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 89 PDB declaration: 94-meric
Chain Ap 1–258（258 aa）
Not recorded MG MAGNESIUM ION × 326 ZN ZINC ION × 6 SPM SPERMINE × 3 FME N-FORMYLMETHIONINE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 5GP GUANOSINE-5'-MONOPHOSPHATE × 2 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.00 Å
6YDW 55S mammalian mitochondrial ribosome with mtEFG1 and two tRNAMet (TI-POST) Deposited 2020-03-21 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 89 PDB declaration: 95-meric
Chain Ap 1–258（258 aa）
Not recorded MG MAGNESIUM ION × 325 ZN ZINC ION × 6 SPM SPERMINE × 3 FME N-FORMYLMETHIONINE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 5GP GUANOSINE-5'-MONOPHOSPHATE × 1 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 4.20 Å
7NQH 55S mammalian mitochondrial ribosome with mtRF1a and P-site tRNAMet Deposited 2021-03-01 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 88 PDB declaration: 93-meric
Chain Ap 1–258（258 aa）
Not recorded MG MAGNESIUM ION × 323 ZN ZINC ION × 6 SPM SPERMINE × 3 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 5GP GUANOSINE-5'-MONOPHOSPHATE × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.50 Å
7NQL 55S mammalian mitochondrial ribosome with ICT1 and P site tRNAMet Deposited 2021-03-01 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 88 PDB declaration: 93-meric
Chain Ap 1–258（258 aa）
Not recorded ZN ZINC ION × 6 MG MAGNESIUM ION × 335 5GP GUANOSINE-5'-MONOPHOSPHATE × 2 SPM SPERMINE × 3 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.40 Å
7NSI 55S mammalian mitochondrial ribosome with mtRRF (pre) and tRNA(P/E) Deposited 2021-03-07 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 88 PDB declaration: 93-meric
Chain Ap 1–258（258 aa）
Not recorded MG MAGNESIUM ION × 327 ZN ZINC ION × 6 SPM SPERMINE × 3 5GP GUANOSINE-5'-MONOPHOSPHATE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 4.60 Å
7NSJ 55S mammalian mitochondrial ribosome with tRNA(P/P) and tRNA(E*) Deposited 2021-03-07 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 87 PDB declaration: 93-meric
Chain Ap 1–258（258 aa）
Not recorded MG MAGNESIUM ION × 324 ZN ZINC ION × 6 SPM SPERMINE × 3 5GP GUANOSINE-5'-MONOPHOSPHATE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.90 Å
8OIN 55S mammalian mitochondrial ribosome with mtRF1 and P-site tRNA Deposited 2023-03-23 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 88 PDB declaration: 94-meric
Chain AO 1–258（258 aa）
Not recorded MG MAGNESIUM ION × 332 K POTASSIUM ION × 41 PHE PHENYLALANINE × 1 ZN ZINC ION × 3 FES FE2/S2 (INORGANIC) CLUSTER × 3 SPM SPERMINE × 1 SPD SPERMIDINE × 1 FME N-FORMYLMETHIONINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20mM HEPES-KOH pH=7.5, 100mM KCl, 20mM MgCl2, ~1% sucrose (from mtRF1 storage buffer)
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.60 Å
8OIP 28S mammalian mitochondrial small ribosomal subunit with mtRF1 and P-site tRNA Deposited 2023-03-23 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 33 PDB declaration: 36-meric
Chain AO 1–258（258 aa）
Not recorded MG MAGNESIUM ION × 127 SPM SPERMINE × 1 SPD SPERMIDINE × 1 K POTASSIUM ION × 11 ZN ZINC ION × 1 FS2 FE-S-O HYBRID CLUSTER × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20mM HEPES-KOH pH=7.5, 100mM KCl, 20mM MgCl2, ~1% sucrose (from mtRF1 storage buffer)
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.60 Å

11 other PDB entries and 11 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name RT18B_PIG
Isoform —
PDB entities 34
Chains and sequence ranges Author chain p； PDBConstruct 1–258； UniProt 1–258

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

SAXS scattering curve SAXS Profile

SAXS profile for 5aj3

P(r) Distance Distribution P(r) Distribution

P(r) distribution for 5aj3
Download Download
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2. Structure Basics 2. Structure Basics

Entry ID entry_id5aj3
Deposition date deposition_date2015-02-20
Structure title titleStructure of the small subunit of the mammalian mitoribosome
Keywords keywords;RIBOSOME, TRANSLATION, MITOCHONDRIA, MAMMALIAN 55S MITORIBOSOME, MAMMALIAN 55S MITOCHONDRIAL RIBOSOME, 28S SMALL SUBUNIT, MRNA, TRNA, DECODING CENTER, CRYO-EM, SINGLE PARTICLE ANALYSIS ;; RIBOSOME
Experimental Method methodELECTRON MICROSCOPY
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3. SAXS Parameters (CRYSOL theoretical calculation) 3. SAXS Parameters (CRYSOL)

Radius of gyration Rg (Guinier) rg_guinier69.88 Å
Radius of gyration Rg (electron density) rg_electron74.20 Å
Forward intensity I(0) i020171400000.00
Molecular weight molecular_weight964820.0 kDa
Excluded volume excluded_volume1108200 ų
Envelope volume envelope_volume1866800 ų
Hydration-shell volume shell_volume206140 ų
Envelope diameter envelope_diameter290.4 Å
Shell Rg shell_rg75.52 Å
Envelope Rg envelope_rg74.37 Å
Shape Rg shape_rg74.38 Å
Total Rg total_rg73.74 Å
Total atoms total_atoms66245
Residues n_residues6484
Spherical-harmonic order n_harmonics20
q range q_range— – 0.5000 Å−1
Data points n_points101
Shell type shell_typedirectional
Solvent electron density solvent_density0.3340 e/ų
Shell contrast contrast_shell0.0300 e/ų
CRYSOL version crysol_version4.1.3
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4. P(r) Distance Distribution (GNOM inversion) 4. P(r) Analysis (GNOM)

Maximum dimension Dmax dmax214.6 Å
Rg (real space) rg_real68.90 Å
Rg uncertainty (real space) rg_real_error1.01 Å
I(0) (real space) i0_real1.9950e+10
I(0) uncertainty (real space) i0_real_error4.0080e+08
Rg (reciprocal space) rg_reciprocal69.36 Å
I(0) (reciprocal space) i0_reciprocal20150000000.0000
Solution quality estimate total_estimate0.8460
Solution quality rating solution_quality GOOD a GOOD solution
P(r) peaks n_peaks1
Primary peak position r_peak_primary78.0 Å
Skewness Skewness skewness0.415
Kurtosis Kurtosis kurtosis-0.237
Angular range angular_range— – 0.1100 Å−1
Current regularization parameter α current_alpha0.2503
Highest regularization parameter α highest_alpha1715000000.0000
Real-space data points n_real_points23
GNOM version gnom_version4.1.3
Quality Criteria quality_criteria AN1: 0.000; Oscil: 0.928; Stabil: 0.998; Sysdev: 1.000; Positv: 1.000; Valcen: 0.971; Smooth: 0.195
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5. Crystallography and Experiment 5. Crystallography & Experiment

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6. Entities and Polymers Entities & Polymers (40)

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7. Fold Classification (SCOP + CATH) 7 domains

CATH v4.4 (7 domains)

Domain ID domain_id5aj3E01
Class class3 — Alpha Beta
Architecture architecture30 — 2-Layer Sandwich
Topology topology230 — Ribosomal Protein S5; domain 2
Homologous superfamily homologous superfamily10 —
Domain ID domain_id5aj3F00
Class class3 — Alpha Beta
Architecture architecture30 — 2-Layer Sandwich
Topology topology70 — Alpha-Beta Plaits
Homologous superfamily homologous superfamily60 — Ribosomal protein S6/Translation elongation factor EF1B
Domain ID domain_id5aj3K00
Class class3 — Alpha Beta
Architecture architecture30 — 2-Layer Sandwich
Topology topology420 — Nucleotidyltransferase; domain 5
Homologous superfamily homologous superfamily80 — Ribosomal protein S11/S14
Domain ID domain_id5aj3L00
Class class2 — Mainly Beta
Architecture architecture40 — Beta Barrel
Topology topology50 — OB fold (Dihydrolipoamide Acetyltransferase, E2P)
Homologous superfamily homologous superfamily140 — Nucleic acid-binding proteins
Domain ID domain_id5aj3N01
Class class1 — Mainly Alpha
Architecture architecture10 — Orthogonal Bundle
Topology topology287 — Helix Hairpins
Homologous superfamily homologous superfamily1480 —
Domain ID domain_id5aj3P00
Class class3 — Alpha Beta
Architecture architecture30 — 2-Layer Sandwich
Topology topology1320 — S16 Ribosomal Protein; Chain: A;
Homologous superfamily homologous superfamily10 — Ribosomal protein S16
Domain ID domain_id5aj3Q00
Class class2 — Mainly Beta
Architecture architecture40 — Beta Barrel
Topology topology50 — OB fold (Dihydrolipoamide Acetyltransferase, E2P)
Homologous superfamily homologous superfamily140 — Nucleic acid-binding proteins
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8. Citations (1)

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9. Files and Curves (10)