5vb8

Crystal structure of the NavAb voltage-gated sodium channel in an open state

Method: X-RAY DIFFRACTION D_max: 96.0 Å Quality: REASONABLE

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1. Protein Identity and Related Structures Protein Identity & Related Structures

Ion transport protein

Arcobacter butzleri

State in the Current Structure

Assembly	Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Associated Components	Method and Experimental Conditions	Structure Quality
1	Protein homooligomer Homooligomer Protein × 4 PDB declaration: tetrameric（4） Consistent with protein copy count	Chain A； UniProt 1–226	Fragment：UNP residues 1-226	NA SODIUM ION × 4 PX4 1,2-DIMYRISTOYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 16 SO4 SULFATE ION × 4	X-RAY DIFFRACTION X-ray crystallization conditions：VAPOR DIFFUSION, HANGING DROP；pH 4.8；277.15 K；1.8 M ammonium sulfate and 100 mM sodium acetate (pH 4.8). 1,2-dimyristoyl-sn-glycero-3-phosphatidylcholine (DMPC):CHAPSO bicelles (Anatrace)	Resolution 2.85 Å R-free 0.254

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB	Difference from Current Entry 5VB8	Assembly / Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Non-polymers	Method and Experimental Conditions	Structure Quality
24JS Crystal structure of voltage-gated sodium channel NavAb N49K mutant Deposited 2026-03-06	Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric	Chain A 1–267（267 aa）	Mutation：N49K	1N7 CHAPSO × 4 PX4 1,2-DIMYRISTOYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 8 LMT DODECYL-BETA-D-MALTOSIDE × 4	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；9%-11% PEG MME 2000, 100mM sodium chloride, 100mM magnesium nitrate, 25mM cadmium nitrate, 100mM Tris-HCl, pH 8.4	Resolution 3.50 Å R-free 0.274
24JT Crystal structure of voltage-gated sodium channel NavAb N49K mutant Deposited 2026-03-06	Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric	Chain A 1–229（229 aa）	Mutation：N49K	LMT DODECYL-BETA-D-MALTOSIDE × 4 1N7 CHAPSO × 4 CA CALCIUM ION × 4	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；7%-9% PEG 6000, 100mM sodium chloride, 100mM magnesium nitrate, 100mM cadmium nitrate, 10mM copper chloride, 100mM Tris-HCl, pH 8.4	Resolution 4.10 Å R-free 0.375
24JU Crystal structure of voltage-gated sodium channel NavAb N49K mutant Deposited 2026-03-06	Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric	Chain A 1–229（229 aa）	Mutation：N49K	LMT DODECYL-BETA-D-MALTOSIDE × 4 1N7 CHAPSO × 4 PX4 1,2-DIMYRISTOYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 32	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；7%-9% PEG 6000, 100mM sodium chloride, 100mM magnesium nitrate, 100mM cadmium nitrate, 10mM copper chloride, 100mM Tris-HCl, pH 8.4	Resolution 3.70 Å R-free 0.320
3RVY Crystal structure of the NavAb voltage-gated sodium channel (Ile217Cys, 2.7 A) Deposited 2011-05-06	Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric	Chain A 1–267（267 aa） Chain B 1–267（267 aa）	Mutation：I217C Mutation：I217C	PX4 1,2-DIMYRISTOYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 20	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 4.75；298 K；CHAPSO:DMPC bicelles, 2 M ammonium sulphate, 0.1 M Na-citrate pH 4.75, 28% glucose, 0.01 M YCl3, nicotinic acid (sat.), VAPOR DIFFUSION, HANGING DROP, temperature 298K	Resolution 2.70 Å R-free 0.273
3RVZ Crystal structure of the NavAb voltage-gated sodium channel (Ile217Cys, 2.8 A) Deposited 2011-05-06	Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric	Chain A 1–267（267 aa） Chain B 1–267（267 aa）	Mutation：I217C Mutation：I217C	PX4 1,2-DIMYRISTOYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 28	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 4.75；298 K；CHAPSO:DMPC bicelles, 2 M ammonium sulphate, 0.1 M Na-citrate pH 4.75, 28% glucose, nicotinic acid (sat.), VAPOR DIFFUSION, HANGING DROP, temperature 298K	Resolution 2.80 Å R-free 0.292
3RW0 Crystal structure of the NavAb voltage-gated sodium channel (Met221Cys, 2.95 A) Deposited 2011-05-06	Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric	Chain A 1–267（267 aa） Chain B 1–267（267 aa）	Mutation：M221C Non-standard monomer：Yes (specific site not provided by mmCIF) Mutation：M221C Non-standard monomer：Yes (specific site not provided by mmCIF)	PX4 1,2-DIMYRISTOYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 20	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 4.75；298 K；CHAPSO:DMPC bicelles, 2 M ammonium sulphate, 0.1 M Na-citrate pH 4.75, 28% glucose, nicotinic acid (sat.), VAPOR DIFFUSION, HANGING DROP, temperature 298K	Resolution 2.95 Å R-free 0.272
4EKW Crystal structure of the NavAb voltage-gated sodium channel (wild-type, 3.2 A) Deposited 2012-04-10	Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric	Chain A 1–267（267 aa） Chain B 1–267（267 aa）	Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF)	PX4 1,2-DIMYRISTOYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 6 PO4 PHOSPHATE ION × 4	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 4.75；298 K；CHAPSO:DMPC BICELLES, 2 M AMMONIUM SULPHATE, 0.1 M NA-CITRATE, 28% GLUCOSE, NICOTINIC ACID, pH 4.75, VAPOR DIFFUSION, HANGING DROP, temperature 298K	Resolution 3.21 Å R-free 0.322
4EKW Crystal structure of the NavAb voltage-gated sodium channel (wild-type, 3.2 A) Deposited 2012-04-10	Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 2 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric	Chain C 1–267（267 aa） Chain D 1–267（267 aa）	Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF)	PX4 1,2-DIMYRISTOYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 PO4 PHOSPHATE ION × 4	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 4.75；298 K；CHAPSO:DMPC BICELLES, 2 M AMMONIUM SULPHATE, 0.1 M NA-CITRATE, 28% GLUCOSE, NICOTINIC ACID, pH 4.75, VAPOR DIFFUSION, HANGING DROP, temperature 298K	Resolution 3.21 Å R-free 0.322
4MS2 Structural basis of Ca2+ selectivity of a voltage-gated calcium channel Deposited 2013-09-18	Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric	Chain A 1–219（219 aa） Chain B 1–219（219 aa） Chain C 1–219（219 aa） Chain D 1–219（219 aa）	Not recorded	PX4 1,2-DIMYRISTOYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 20 CA CALCIUM ION × 5	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 4.75；298 K；0.1M Na-citrate, pH 4.75, 2M ammonium sulfate, VAPOR DIFFUSION, HANGING DROP, temperature 298K	Resolution 2.75 Å R-free 0.255
4MTF Structural Basis of Ca2+ Selectivity of a Voltage-gated Calcium Channel Deposited 2013-09-19	Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric	Chain A 1–219（219 aa） Chain B 1–219（219 aa） Chain C 1–219（219 aa） Chain D 1–219（219 aa）	Not recorded	CA CALCIUM ION × 2 PX4 1,2-DIMYRISTOYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 16	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 4.75；298 K；0.1M Na-citrate,pH4.75, 2M ammonium sulfate, VAPOR DIFFUSION, HANGING DROP, temperature 298K	Resolution 3.20 Å R-free 0.272
4MTG Structural Basis for Ca2+ Selectivity of a Voltage-gated Calcium Channel Deposited 2013-09-19	Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric	Chain A 1–219（219 aa） Chain B 1–219（219 aa） Chain C 1–219（219 aa） Chain D 1–219（219 aa）	Not recorded	CA CALCIUM ION × 2 PX4 1,2-DIMYRISTOYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 16	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 4.75；298 K；0.1M Na-citrate,pH 4.75, 2M ammonium sulfate, VAPOR DIFFUSION, HANGING DROP, temperature 298K	Resolution 3.30 Å R-free 0.273
4MTO Structural Basis for Ca2+ Selectivity of a Voltage-gated Calcium Channel Deposited 2013-09-19	Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric	Chain A 1–219（219 aa） Chain B 1–219（219 aa） Chain C 1–219（219 aa） Chain D 1–219（219 aa）	Not recorded	CA CALCIUM ION × 3 PX4 1,2-DIMYRISTOYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 16	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 4.75；298 K；0.1M Na-citrate,pH 4.75, 2M ammonium sulfate, VAPOR DIFFUSION, HANGING DROP, temperature 298K	Resolution 3.40 Å R-free 0.297
4MVM Structural Basis for Ca2+ Selectivity of a Voltage-gated Calcium Channel Deposited 2013-09-24	Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric	Chain A 1–219（219 aa） Fragment：Voltage-gated Calcium Channel (Residues 1-219) Chain B 1–219（219 aa） Fragment：Voltage-gated Calcium Channel (Residues 1-219) Chain C 1–219（219 aa） Fragment：Voltage-gated Calcium Channel (Residues 1-219) Chain D 1–219（219 aa） Fragment：Voltage-gated Calcium Channel (Residues 1-219)	Not recorded	PX4 1,2-DIMYRISTOYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 16 CA CALCIUM ION × 5	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 4.75；298 K；CHAPSO:DMPC BICELLES,0.1M Na-Acetate ,pH4.75, 2M Ammonium Sulfate, 28% Glucose, 10mM Calcium Chloride., VAPOR DIFFUSION, HANGING DROP, temperature 298K	Resolution 3.20 Å R-free 0.275
4MVO Structural Basis for Ca2+ Selectivity of a Voltage-gated Calcium Channel Deposited 2013-09-24	Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric	Chain A 1–219（219 aa） Fragment：Voltage-gated Calcium Channel (Residues 1-219) Chain B 1–219（219 aa） Fragment：Voltage-gated Calcium Channel (Residues 1-219) Chain C 1–219（219 aa） Fragment：Voltage-gated Calcium Channel (Residues 1-219) Chain D 1–219（219 aa） Fragment：Voltage-gated Calcium Channel (Residues 1-219)	Not recorded	PX4 1,2-DIMYRISTOYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 16 CA CALCIUM ION × 3	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 4.75；298 K；CHAPSO:DMPC BICELLES, 0.1M Na-Acetate, pH4.75, 2M Ammonium Sulfate, 28% Glucose, 15mM Calcium Chloride., VAPOR DIFFUSION, HANGING DROP, temperature 298K	Resolution 3.30 Å R-free 0.279
4MVQ Structural Basis for Ca2+ Selectivity of a Voltage-gated Calcium Channel Deposited 2013-09-24	Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric	Chain A 1–219（219 aa） Chain B 1–219（219 aa） Chain C 1–219（219 aa） Chain D 1–219（219 aa）	Not recorded	CA CALCIUM ION × 2 PX4 1,2-DIMYRISTOYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 16	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 4.75；298 K；CHAPSO:DMPC BICELLES,0.1M Na-Acetate ,pH4.75, 2M Ammonium Sulfate, 28% Glucose, 15mM Calcium Chloride., VAPOR DIFFUSION, HANGING DROP, temperature 298K	Resolution 3.40 Å R-free 0.313
4MVR Structural Basis for Ca2+ Selectivity of a Voltage-gated Calcium Channel Deposited 2013-09-24	Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric	Chain A 1–219（219 aa） Chain B 1–219（219 aa） Chain C 1–219（219 aa） Chain D 1–219（219 aa）	Not recorded	MN MANGANESE (II) ION × 1 PX4 1,2-DIMYRISTOYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 16	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 5；298 K；CHAPSO:DMPC BICELLES, 0.1M Na-Acetate, pH5.0, 2M Ammonium Sulfate, 28% Glucose, 10mM Mn2+, VAPOR DIFFUSION, HANGING DROP, temperature 298K	Resolution 3.20 Å R-free 0.262
4MVS Structural Basis for Ca2+ Selectivity of a Voltage-gated Calcium Channel Deposited 2013-09-24	Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric	Chain A 1–219（219 aa） Chain B 1–219（219 aa） Chain C 1–219（219 aa） Chain D 1–219（219 aa）	Not recorded	CD CADMIUM ION × 5 PX4 1,2-DIMYRISTOYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 16	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 4.75；298 K；CHAPSO:DMPC bicelles, 0.1M Na-Acetate, pH4.75, 2M Ammonium Sulfate, 28% Glucose, 100mM Mn2+, VAPOR DIFFUSION, HANGING DROP, temperature 298K	Resolution 3.30 Å R-free 0.269
4MVU Structural Basis for Ca2+ Selectivity of a Voltage-gated Calcium Channel Deposited 2013-09-24	Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric	Chain A 1–219（219 aa） Chain B 1–219（219 aa） Chain C 1–219（219 aa） Chain D 1–219（219 aa）	Not recorded	PX4 1,2-DIMYRISTOYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 16 CA CALCIUM ION × 3	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 4.75；298 K；CHAPSO:DMPC bicelles, 0.1M Na-Acetate, pH4.75, 2M Ammonium Sulfate, 28% Glucose, 15mM Calcium Chloride, VAPOR DIFFUSION, HANGING DROP, temperature 298K	Resolution 3.20 Å R-free 0.263
4MVZ Structural Basis for Ca2+ Selectivity of a Voltage-gated Calcium Channel Deposited 2013-09-24	Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric	Chain A 1–219（219 aa） Chain B 1–219（219 aa） Chain C 1–219（219 aa） Chain D 1–219（219 aa）	Not recorded	CA CALCIUM ION × 2 PX4 1,2-DIMYRISTOYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 16	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 4.75；298 K；CHAPSO:DMPC bicelles, 0.1M Na-Acetate, pH 4.75, 2M Ammonium Sulfate, 28% Glucose, 15mM Calcium Chloride, VAPOR DIFFUSION, HANGING DROP, temperature 298K	Resolution 3.30 Å R-free 0.280
4MW3 Structural Basis for Ca2+ Selectivity of a Voltage-gated Calcium Channel Deposited 2013-09-24	Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric	Chain A 1–219（219 aa） Chain B 1–219（219 aa） Chain C 1–219（219 aa） Chain D 1–219（219 aa）	Not recorded	CA CALCIUM ION × 3 PX4 1,2-DIMYRISTOYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 16	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 4.75；298 K；CHAPSO:DMPC bicelles, 0.1M Na-Acetate,pH 4.75, 2M Ammonium Sulfate, 28% Glucose, 15mM Calcium Chloride, VAPOR DIFFUSION, HANGING DROP, temperature 298K	Resolution 3.30 Å R-free 0.267
4MW8 Structural Basis for Ca2+ Selectivity of a Voltage-gated Calcium Channel Deposited 2013-09-24	Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric	Chain A 1–219（219 aa） Chain B 1–219（219 aa） Chain C 1–219（219 aa） Chain D 1–219（219 aa）	Not recorded	CA CALCIUM ION × 2 PX4 1,2-DIMYRISTOYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 16	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 4.75；298 K；CHAPSO:DMPC bicelles, 0.1M Na-Acetate,pH 4.75, 2M Ammonium Sulfate, 28% Glucose, 15mM Calcium Chloride, VAPOR DIFFUSION, HANGING DROP, temperature 298K	Resolution 3.26 Å R-free 0.314
5EK0 Human Nav1.7-VSD4-NavAb in complex with GX-936. Deposited 2015-11-03	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Insufficient information Homooligomer；Protein × 4 PDB declaration: tetrameric	Chain A 1–23（23 aa） Chain A 58–78（21 aa） Chain A 109–267（159 aa） Chain B 1–23（23 aa） Chain B 58–78（21 aa） Chain B 109–267（159 aa） Chain C 1–23（23 aa） Chain C 58–78（21 aa） Chain C 109–267（159 aa） Chain D 1–23（23 aa） Chain D 58–78（21 aa） Chain D 109–267（159 aa）	Not recorded	PX4 1,2-DIMYRISTOYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 21 5P2 3-cyano-4-[2-[2-(1-ethylazetidin-3-yl)pyrazol-3-yl]-4-(trifluoromethyl)phenoxy]-~{N}-(1,2,4-thiadiazol-5-yl)benzenesulfonamide × 4	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 5.4；277 K；2.2 ammonium sulfate, 100 mM sodium citrate pH 5.4	Resolution 3.53 Å R-free 0.272
5KLB Crystal structure of the CavAb voltage-gated calcium channel(wild-type, 2.7A) Deposited 2016-06-23	Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric	Chain A 1–267（267 aa） Chain B 1–267（267 aa） Chain C 1–267（267 aa） Chain D 1–267（267 aa）	Not recorded	MC3 1,2-DIMYRISTOYL-RAC-GLYCERO-3-PHOSPHOCHOLINE × 20 CA CALCIUM ION × 3 CPS 3-[(3-CHOLAMIDOPROPYL)DIMETHYLAMMONIO]-1-PROPANESULFONATE × 6	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 5；298 K；CHAPSO:DMPC BICELLES,0.1M Na-citrate,pH5.0,2M Ammonium Sulfate	Resolution 2.70 Å R-free 0.260
5KLG Structure of CavAb(W195Y) in complex with Br-dihydropyridine derivative UK-59811 Deposited 2016-06-24	Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric	Chain A 1–267（267 aa） Chain B 1–267（267 aa） Chain C 1–267（267 aa） Chain D 1–267（267 aa）	Not recorded	CA CALCIUM ION × 2 MC3 1,2-DIMYRISTOYL-RAC-GLYCERO-3-PHOSPHOCHOLINE × 11 6UC O3-ethyl O5-methyl (4R)-4-(2-bromophenyl)-2-[2-(dimethylamino)ethoxymethyl]-6-methyl-1,4-dihydropyridine-3,5-dicarboxylate × 2	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 5；298 K；CHAPSO:DMPC BICELLES,0.1M Na-citrate,pH5.0,2M Ammonium Sulfate	Resolution 3.30 Å R-free 0.303
5KLS Structure of CavAb in complex with Br-dihydropyridine derivative UK-59811 Deposited 2016-06-25	Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric	Chain A 1–267（267 aa） Chain B 1–267（267 aa） Chain C 1–267（267 aa） Chain D 1–267（267 aa）	Not recorded	MC3 1,2-DIMYRISTOYL-RAC-GLYCERO-3-PHOSPHOCHOLINE × 15 CA CALCIUM ION × 2 6UC O3-ethyl O5-methyl (4R)-4-(2-bromophenyl)-2-[2-(dimethylamino)ethoxymethyl]-6-methyl-1,4-dihydropyridine-3,5-dicarboxylate × 1	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 5；298 K；CHAPSO:DMPC BICELLES,0.1M Na-citrate,pH5.0,2M Ammonium Sulfate 100uM UK-59811	Resolution 3.30 Å R-free 0.300
5KMD Structure of CavAb in complex with amlodipine Deposited 2016-06-26	Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric	Chain A 1–267（267 aa） Chain B 1–267（267 aa） Chain C 1–267（267 aa） Chain D 1–267（267 aa）	Not recorded	MC3 1,2-DIMYRISTOYL-RAC-GLYCERO-3-PHOSPHOCHOLINE × 14 CA CALCIUM ION × 2 6UB amlodipine × 1	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 5；298 K；CHAPSO:DMPC BICELLES,0.1M Na-citrate,pH5.0,2M Ammonium Sulfate ,100uM amlodipine	Resolution 3.20 Å R-free 0.277
5KMF Structure of CavAb in complex with nimodipine Deposited 2016-06-26	Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric	Chain A 1–267（267 aa） Chain B 1–267（267 aa） Chain C 1–267（267 aa） Chain D 1–267（267 aa）	Not recorded	6U9 S-nimodipine × 1 MC3 1,2-DIMYRISTOYL-RAC-GLYCERO-3-PHOSPHOCHOLINE × 14 CA CALCIUM ION × 2	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 5；298 K；CHAPSO:DMPC BICELLES,0.1M Na-citrate,pH5.0,2M Ammonium Sulfate,100uM nimodipine	Resolution 3.20 Å R-free 0.256
5KMH Structure of CavAb in complex with Br-verapamil Deposited 2016-06-27	Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric	Chain A 1–267（267 aa） Chain B 1–267（267 aa） Chain C 1–267（267 aa） Chain D 1–267（267 aa）	Not recorded	CA CALCIUM ION × 3 MC3 1,2-DIMYRISTOYL-RAC-GLYCERO-3-PHOSPHOCHOLINE × 6 PX6 1,2-DIPALMITOYL-SN-GLYCERO-3-PHOSPHATE × 4 6U8 (2~{R})-2-(2-bromophenyl)-5-[2-(3,4-dimethoxyphenyl)ethyl-methyl-amino]-2-propan-2-yl-pentanenitrile × 1	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 5；298 K；CHAPSO:DMPC BICELLES,0.1M Na-citrate,pH5.0,2M Ammonium Sulfate,100uM Br-verapamil	Resolution 3.20 Å R-free 0.294
5VB2 Crystal structure of the NavAb voltage-gated sodium channel in a closed conformation Deposited 2017-03-28	Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric	Chain A 1–267（267 aa） Chain B 1–267（267 aa） Chain C 1–267（267 aa） Chain D 1–267（267 aa）	Mutation：T206F, V213Y Mutation：T206F, V213Y Mutation：T206F, V213Y Mutation：T206F, V213Y	CPS 3-[(3-CHOLAMIDOPROPYL)DIMETHYLAMMONIO]-1-PROPANESULFONATE × 8 PX4 1,2-DIMYRISTOYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 13 PO4 PHOSPHATE ION × 4 CL CHLORIDE ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；277.15 K；1.8 M ammonium sulfate and 100 mM sodium acetate, 1,2-dimyristoyl-sn-glycero-3-phosphatidylcholine (DMPC):CHAPSO bicelles	Resolution 3.20 Å R-free 0.266
6C1E NavAb NormoPP mutant Deposited 2018-01-04	Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric	Chain A 1–267（267 aa） Chain B 1–267（267 aa）	Mutation：C235I, H123R Mutation：C235I, H123R	PX4 1,2-DIMYRISTOYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 36 BNC 5-BETA-24-NOR-CHOLANE-3(ALPHA),7(ALPHA),12(ALPHA)-TRIOL × 2 UHH (3ALPHA,5ALPHA,7ALPHA,8ALPHA,12ALPHA,14BETA,17ALPHA)-3,7,12-TRIHYDROXYCHOL-1-EN-24-AMIDE × 2	X-RAY DIFFRACTION X-ray crystallization conditions EVAPORATION；277 K；ammonium sulphate, Na-citrate	Resolution 2.86 Å R-free 0.240
6C1K HypoPP mutant with ligand1 Deposited 2018-01-04	Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric	Chain A 1–267（267 aa） Chain B 1–267（267 aa）	Mutation：C235I, H123R Mutation：C235I, H123R	GAI GUANIDINE × 4 PX4 1,2-DIMYRISTOYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 34 NA SODIUM ION × 8 SO4 SULFATE ION × 4	X-RAY DIFFRACTION X-ray crystallization conditions EVAPORATION；277 K；ammonium sulphate, Na-citrate	Resolution 2.70 Å R-free 0.246
6C1M NavAb NormoPP mutant Deposited 2018-01-04	Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric	Chain A 1–267（267 aa） Chain B 1–267（267 aa）	Mutation：C235I, H123R Mutation：C235I, H123R	PX4 1,2-DIMYRISTOYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 30 1N7 CHAPSO × 16 MGX 1-METHYLGUANIDINE × 4 NA SODIUM ION × 8 SO4 SULFATE ION × 4	X-RAY DIFFRACTION X-ray crystallization conditions EVAPORATION；pH 4.8；277 K；ammonium sulphate, Na-citrate	Resolution 2.52 Å R-free 0.227
6C1P HypoPP mutant Deposited 2018-01-05	Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric	Chain A 1–267（267 aa） Chain B 1–267（267 aa） Chain C 1–267（267 aa） Chain D 1–267（267 aa）	Mutation：C235I, H123R Mutation：C235I, H123R Mutation：C235I, H123R Mutation：C235I, H123R	PO4 PHOSPHATE ION × 2 PX4 1,2-DIMYRISTOYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 15 1N7 CHAPSO × 1	X-RAY DIFFRACTION X-ray crystallization conditions EVAPORATION；277 K；ammonium sulphate, Na-citrate	Resolution 2.90 Å R-free 0.260
6JUH structure of CavAb in complex with efonidipine Deposited 2019-04-13	Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric	Chain A 1–267（267 aa） Chain B 1–267（267 aa） Chain C 1–267（267 aa） Chain D 1–267（267 aa）	Mutation：E1177D, S1178D, M1181N Mutation：E1177D, S1178D, M1181N Mutation：E1177D, S1178D, M1181N Mutation：E1177D, S1178D, M1181N	MC3 1,2-DIMYRISTOYL-RAC-GLYCERO-3-PHOSPHOCHOLINE × 4 PX4 1,2-DIMYRISTOYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 G3P SN-GLYCEROL-3-PHOSPHATE × 4 CA CALCIUM ION × 2 C9X 2-[phenyl-(phenylmethyl)amino]ethyl (4~{R})-5-(5,5-dimethyl-2-oxidanylidene-1,3,2$l^{5}-dioxaphosphinan-2-yl)-2,6-dimethyl-4-(3-nitrophenyl)-1,4-dihydropyridine-3-carboxylate × 1	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；277.15 K；1.0-2.0M Ammonium Sulfate 100 mM Na-citrate pH=5.0	Resolution 3.00 Å R-free 0.291
6KE5 Structure of CavAb in complex with Diltiazem and Amlodipine Deposited 2019-07-03	Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric	Chain A 1–267（267 aa） Chain B 1–267（267 aa） Chain C 1–267（267 aa） Chain D 1–267（267 aa）	Not recorded	LPC [1-MYRISTOYL-GLYCEROL-3-YL]PHOSPHONYLCHOLINE × 3 CA CALCIUM ION × 3 G3P SN-GLYCEROL-3-PHOSPHATE × 5 6UB amlodipine × 1 D6C [(2~{S},3~{R})-5-[2-(dimethylamino)ethyl]-2-(4-methoxyphenyl)-4-oxidanylidene-2,3-dihydro-1,5-benzothiazepin-3-yl] ethanoate × 1	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 5；298 K；0.1M Na-citrate,pH5.0,2M Ammonium Sulfate	Resolution 2.80 Å R-free 0.292
6KEB Structure basis for Diltiazem block of a voltage-gated calcium channel Deposited 2019-07-04	Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric	Chain A 1–267（267 aa） Chain B 1–267（267 aa） Chain C 1–267（267 aa） Chain D 1–267（267 aa）	Not recorded	PX4 1,2-DIMYRISTOYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 12 CA CALCIUM ION × 2 D6C [(2~{S},3~{R})-5-[2-(dimethylamino)ethyl]-2-(4-methoxyphenyl)-4-oxidanylidene-2,3-dihydro-1,5-benzothiazepin-3-yl] ethanoate × 1	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 5；298 K；CHAPSO:DMPC BICELLES,0.1M Na-citrate,pH5.0,2M Ammonium Sulfate	Resolution 3.20 Å R-free 0.277
6MVV NavAb voltage-gated sodium channel, I217C/F203A Deposited 2018-10-28	Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric	Chain A 1–267（267 aa） Chain B 1–267（267 aa） Chain C 1–267（267 aa） Chain D 1–267（267 aa）	Mutation：F203A, I217C Mutation：F203A, I217C Mutation：F203A, I217C Mutation：F203A, I217C	PX4 1,2-DIMYRISTOYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 6 PO4 PHOSPHATE ION × 5	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；277.15 K；1.8 M Ammonium Sulfate 100mM Sodium Acetate, pH 5.0	Resolution 2.90 Å R-free 0.256
6MVW NavAb voltage-gated sodium channel, I217C/F203W Deposited 2018-10-28	Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric	Chain A 1–267（267 aa） Chain B 1–267（267 aa） Chain C 1–267（267 aa） Chain D 1–267（267 aa）	Mutation：I217C, F203W Mutation：I217C, F203W Mutation：I217C, F203W Mutation：I217C, F203W	PX4 1,2-DIMYRISTOYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 10 PO4 PHOSPHATE ION × 8	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；277 K；1.8 M Ammonium Sulfate, 100 mM Sodium Acetate	Resolution 3.20 Å R-free 0.246
6MVX NavAb Voltage-gated Sodium Channel, I217C, in Complex with Class 1C Anti-arrhythmic Flecainide Deposited 2018-10-28	Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric	Chain A 1–267（267 aa） Chain B 1–267（267 aa） Chain C 1–267（267 aa） Chain D 1–267（267 aa）	Mutation：I217C Mutation：I217C Mutation：I217C Mutation：I217C	PO4 PHOSPHATE ION × 10 K4D Flecainide × 1	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；277 K；1.8 M Ammonium Sulfate 100 mM Sodium Acetate, pH 5.0 100 uM Flecainide	Resolution 3.46 Å R-free 0.263
6MVY NavAb voltage-gated sodium channel, residues 1-226, crystallized in the presence of Class 1B Anti-arrhythmic drug Lidocaine Deposited 2018-10-28	Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric	Chain A 1–226（226 aa）	Mutation：I217C	PX4 1,2-DIMYRISTOYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 16 SO4 SULFATE ION × 4	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；277 K；1.8 M Ammonium Sulfate 100 mM Sodium Acetate, pH 5.0 10 mM Lidocaine	Resolution 3.00 Å R-free 0.237
6MVY NavAb voltage-gated sodium channel, residues 1-226, crystallized in the presence of Class 1B Anti-arrhythmic drug Lidocaine Deposited 2018-10-28	Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 2 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric	Chain B 1–226（226 aa）	Mutation：I217C	PX4 1,2-DIMYRISTOYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 16 SO4 SULFATE ION × 4	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；277 K；1.8 M Ammonium Sulfate 100 mM Sodium Acetate, pH 5.0 10 mM Lidocaine	Resolution 3.00 Å R-free 0.237
6MWA NavAb Voltage-gated Sodium Channel, residues 1-239 Deposited 2018-10-29	Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric	Chain B 1–239（239 aa）	Not recorded	PX4 1,2-DIMYRISTOYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 16 SO4 SULFATE ION × 4 CPS 3-[(3-CHOLAMIDOPROPYL)DIMETHYLAMMONIO]-1-PROPANESULFONATE × 8	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 5.8；277 K；1.8 M Ammonium Sulfate 100mM Sodium Acetate pH 5.8	Resolution 2.40 Å R-free 0.249
6MWB NavAb Voltage-gated Sodium Channel, residues 1-239 with mutation T206A Deposited 2018-10-29	Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric	Chain B 1–239（239 aa）	Mutation：T206A	PX4 1,2-DIMYRISTOYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 16 SO4 SULFATE ION × 4 CPS 3-[(3-CHOLAMIDOPROPYL)DIMETHYLAMMONIO]-1-PROPANESULFONATE × 8	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 5.8；277 K；1.8 M Ammonium Sulfate 100 mM Sodium Acetate pH 5.8	Resolution 2.60 Å R-free 0.236
6MWD NavAb Voltage-gated Sodium Channel, residues 1-239 with mutation T206S Deposited 2018-10-29	Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric	Chain B 1–239（239 aa）	Mutation：T206S	PX4 1,2-DIMYRISTOYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 16 ACT ACETATE ION × 4 SO4 SULFATE ION × 4 CPS 3-[(3-CHOLAMIDOPROPYL)DIMETHYLAMMONIO]-1-PROPANESULFONATE × 12	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 5.8；277 K；1.8 Ammonium Sulfate 100 mM Sodium Acetate, pH 5.8	Resolution 2.33 Å R-free 0.250
6MWG NavAb Voltage-gated Sodium Channel, residues 1-239, with mutation T206V Deposited 2018-10-29	Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric	Chain B 1–239（239 aa）	Mutation：T206V	PX4 1,2-DIMYRISTOYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 16 ACT ACETATE ION × 4 CPS 3-[(3-CHOLAMIDOPROPYL)DIMETHYLAMMONIO]-1-PROPANESULFONATE × 8	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 5.8；277 K；1.8 M Ammonium Sulfate 100 mM Sodium Acetate, pH 5.8	Resolution 2.50 Å R-free 0.239
6N4Q CryoEM structure of Nav1.7 VSD2 (actived state) in complex with the gating modifier toxin ProTx2 Deposited 2018-11-20	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Insufficient information Heteromer；Protein × 12 PDB declaration: dodecameric	Chain A 1–25（25 aa） Chain A 58–78（21 aa） Chain A 107–267（161 aa） Chain B 1–25（25 aa） Chain B 58–78（21 aa） Chain B 107–267（161 aa） Chain C 1–25（25 aa） Chain C 58–78（21 aa） Chain C 107–267（161 aa） Chain D 1–25（25 aa） Chain D 58–78（21 aa） Chain D 107–267（161 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 8；10 mM Tris pH 8.0, 100 mM NaCl, 0.06% FA3, 0.1 mg/ml POPC:POPE:POPG mixed at molar ratio 3:1:1 cryo-EM vitrification conditions Cryogen ETHANE；Apply 3 uL, blot 2.5s. Ted Pella 595 filter paper.	Resolution 3.60 Å
6N4R CryoEM structure of Nav1.7 VSD2 (deactived state) in complex with the gating modifier toxin ProTx2 Deposited 2018-11-20	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Insufficient information Heteromer；Protein × 12 PDB declaration: dodecameric	Chain A 1–25（25 aa） Chain A 58–78（21 aa） Chain A 107–267（161 aa） Chain B 1–25（25 aa） Chain B 58–78（21 aa） Chain B 107–267（161 aa） Chain C 1–25（25 aa） Chain C 58–78（21 aa） Chain C 107–267（161 aa） Chain D 1–25（25 aa） Chain D 58–78（21 aa） Chain D 107–267（161 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 8；10 mM Tris pH 8.0, 100 mM NaCl, 0.06% FA3, 0.1 mg/ml POPC:POPE:POPG mixed at molar ratio 3:1:1 cryo-EM vitrification conditions Cryogen ETHANE；Apply 3 uL, blot 2.5s. Ted Pella 595 filter paper.	Resolution 4.20 Å
6P6W Cryo-EM structure of voltage-gated sodium channel NavAb N49K/L109A/M116V/G94C/Q150C disulfide crosslinked mutant in the resting state Deposited 2019-06-04	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Insufficient information Homooligomer；Protein × 4 PDB declaration: tetrameric	Chain A 1–267（267 aa） Chain B 1–267（267 aa） Chain C 1–267（267 aa） Chain D 1–267（267 aa）	Mutation：R4A, N49K, L109A, M116V, G94C, Q150C,R4A, N49K, L109A, M116V, G94C, Q150C Mutation：R4A, N49K, L109A, M116V, G94C, Q150C,R4A, N49K, L109A, M116V, G94C, Q150C Mutation：R4A, N49K, L109A, M116V, G94C, Q150C,R4A, N49K, L109A, M116V, G94C, Q150C Mutation：R4A, N49K, L109A, M116V, G94C, Q150C,R4A, N49K, L109A, M116V, G94C, Q150C	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 8 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 4.00 Å
6P6X Crystal structure of voltage-gated sodium channel NavAb G94C/Q150C mutant in the activated state Deposited 2019-06-04	Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric	Chain A 1–239（239 aa）	Mutation：G94C, Q150C	PX4 1,2-DIMYRISTOYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 36 CPS 3-[(3-CHOLAMIDOPROPYL)DIMETHYLAMMONIO]-1-PROPANESULFONATE × 8	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 5.6；277 K；1.8 M ammonium sulfate 0.1 M sodium citrate pH 5.6	Resolution 2.75 Å R-free 0.265
6P6Y Crystal structure of voltage-gated sodium channel NavAb V100C/Q150C disulfide crosslinked mutant in the activated state Deposited 2019-06-04	Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric	Chain A 1–239（239 aa）	Mutation：V100C, Q150C	CPS 3-[(3-CHOLAMIDOPROPYL)DIMETHYLAMMONIO]-1-PROPANESULFONATE × 8 PX4 1,2-DIMYRISTOYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 20	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 4.8；277 K；1.8 M ammonium sulfate 0.1 M sodium citrate pH 4.8	Resolution 2.89 Å R-free 0.273
7K48 Structure of NavAb/Nav1.7-VS2A chimera trapped in the resting state by tarantula toxin m3-Huwentoxin-IV Deposited 2020-09-15	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Insufficient information Heteromer；Protein × 8 PDB declaration: octameric	Chain A 1–17（17 aa） Chain A 60–83（24 aa） Chain A 106–239（134 aa） Chain B 1–17（17 aa） Chain B 60–83（24 aa） Chain B 106–239（134 aa） Chain C 1–17（17 aa） Chain C 60–83（24 aa） Chain C 106–239（134 aa） Chain D 1–17（17 aa） Chain D 60–83（24 aa） Chain D 106–239（134 aa）	Mutation：R398A,L506A,M513V Mutation：R398A,L506A,M513V Mutation：R398A,L506A,M513V Mutation：R398A,L506A,M513V Mutation：R398A,L506A,M513V Mutation：R398A,L506A,M513V Mutation：R398A,L506A,M513V Mutation：R398A,L506A,M513V Mutation：R398A,L506A,M513V Mutation：R398A,L506A,M513V Mutation：R398A,L506A,M513V Mutation：R398A,L506A,M513V	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE；Blot for 2.5-4.0 seconds before plunging	Resolution 3.60 Å
8DIV Crystal structure of NavAb I22V as a basis for the human Nav1.7 Inherited Erythromelalgia I136V mutation Deposited 2022-06-29	Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric	Chain A 1–239（239 aa）	Mutation：I22V	BGC beta-D-glucopyranose × 4 PX4 1,2-DIMYRISTOYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 20 CPS 3-[(3-CHOLAMIDOPROPYL)DIMETHYLAMMONIO]-1-PROPANESULFONATE × 8	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 5；277 K；1.7-1.8 M Ammonium sulfate 0.1 M Sodium Citrate pH 5.0	Resolution 2.54 Å R-free 0.237
8DIW Crystal structure of NavAb E96P as a basis for the human Nav1.7 Inherited Erythromelalgia S211P mutation Deposited 2022-06-29	Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric	Chain A 1–239（239 aa）	Mutation：E96P	PX4 1,2-DIMYRISTOYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 20 CPS 3-[(3-CHOLAMIDOPROPYL)DIMETHYLAMMONIO]-1-PROPANESULFONATE × 8	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 5.4；277 K；1.7-1.8 M Ammonium Sulfate 0.1 M Sodium Citrate pH 5.4	Resolution 3.11 Å R-free 0.272
8DIX Structure of NavAb L98R as a basis for the human Nav1.7 Inherited Erythromelalgia L823R mutation Deposited 2022-06-29	Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric	Chain A 1–239（239 aa）	Mutation：L98R	PX4 1,2-DIMYRISTOYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 20	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 4.8；277 K；1.8-1.9 M Ammonium Sulfate 0.1 M Sodium Citrate pH 4.8	Resolution 3.30 Å R-free 0.255
8DIY Crystal structure of NavAb L101S as a basis for the human Nav1.7 Inherited Erythromelalgia F216S mutation Deposited 2022-06-29	Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric	Chain A 1–239（239 aa）	Mutation：L101S	PX4 1,2-DIMYRISTOYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 20 CPS 3-[(3-CHOLAMIDOPROPYL)DIMETHYLAMMONIO]-1-PROPANESULFONATE × 8	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 4.8；277 K；1.7-1.8 M Ammonium Sulfate 0.1 M Sodium Citrate pH 4.8	Resolution 2.85 Å R-free 0.254
8DIZ Crystal structure of NavAb I119T as a basis for the human Nav1.7 Inherited Erythromelalgia I234T mutation Deposited 2022-06-29	Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric	Chain A 1–239（239 aa）	Mutation：I119T	PX4 1,2-DIMYRISTOYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 20	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 4.6；277 K；1.8-1.9 M Ammonium Sulfate 0.1 M Sodium Citrate pH 4.6	Resolution 2.75 Å R-free 0.255
8DJ0 Crystal structure of NavAb L123T as a basis for the human Nav1.7 Inherited Erythromelalgia I848T mutation Deposited 2022-06-29	Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric	Chain A 1–239（239 aa）	Mutation：L123T	PX4 1,2-DIMYRISTOYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 20 CPS 3-[(3-CHOLAMIDOPROPYL)DIMETHYLAMMONIO]-1-PROPANESULFONATE × 8	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 4.6；277 K；1.9 M Ammonium sulfate 0.1 M Sodium Citrate pH 4.6	Resolution 2.70 Å R-free 0.248
8DJ1 Crystal structure of NavAb V126T as a basis for the human Nav1.7 Inherited Erythromelalgia S241T mutation Deposited 2022-06-29	Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric	Chain A 1–239（239 aa）	Mutation：V126T	PX4 1,2-DIMYRISTOYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 20 CPS 3-[(3-CHOLAMIDOPROPYL)DIMETHYLAMMONIO]-1-PROPANESULFONATE × 8	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 5.2；277 K；1.8 M Ammonium Sulfate 0.1 M Sodium Citrate pH 5.2	Resolution 3.10 Å R-free 0.260
8H9O Crystal structure of voltage-gated sodium channel NavAb N49K mutant in sodium ion condition Deposited 2022-10-25	Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric	Chain A 1–267（267 aa）	Mutation：N49K	LMT DODECYL-BETA-D-MALTOSIDE × 8 1N7 CHAPSO × 4 PX4 1,2-DIMYRISTOYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 36 NA SODIUM ION × 4	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；293 K；9%-11% PEG MME 2000, 100 mM sodium chloride, 100 mM magnesium nitrate, 25 mM cadmium nitrate, 100 mM Tris-HCl, pH 8.4	Resolution 3.30 Å R-free 0.272
8H9W Crystal structure of voltage-gated sodium channel NavAb N49K mutant in calcium ion condition Deposited 2022-10-25	Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric	Chain A 1–267（267 aa）	Mutation：N49K	LMT DODECYL-BETA-D-MALTOSIDE × 8 1N7 CHAPSO × 4 PX4 1,2-DIMYRISTOYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 32 CA CALCIUM ION × 4	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；9%-11% PEG MME 2000, 100 mM sodium chloride, 100 mM magnesium nitrate, 25 mM cadmium nitrate, 100 mM Tris-HCl, pH 8.4	Resolution 2.70 Å R-free 0.264
8H9X Crystal structure of voltage-gated sodium channel NavAb N49K/L176Q mutant in sodium ion condition Deposited 2022-10-25	Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric	Chain A 1–267（267 aa）	Mutation：N49K, L176Q	LMT DODECYL-BETA-D-MALTOSIDE × 8 1N7 CHAPSO × 4 PX4 1,2-DIMYRISTOYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 32 NA SODIUM ION × 8	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；9%-11% PEG MME 2000, 100 mM sodium chloride, 100 mM magnesium nitrate, 25 mM cadmium nitrate, 100 mM Tris-HCl, pH 8.4	Resolution 3.40 Å R-free 0.280
8H9Y Crystal structure of voltage-gated sodium channel NavAb N49K/L176Q mutant in calcium ion condition Deposited 2022-10-25	Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric	Chain A 1–267（267 aa）	Mutation：N49K, L176Q	LMT DODECYL-BETA-D-MALTOSIDE × 8 1N7 CHAPSO × 4 PX4 1,2-DIMYRISTOYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 32 CA CALCIUM ION × 8	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；9%-11% PEG MME 2000, 100 mM sodium chloride, 100 mM magnesium nitrate, 25 mM cadmium nitrate, 100 mM Tris-HCl, pH 8.4	Resolution 3.40 Å R-free 0.264
8HA1 Crystal structure of voltage-gated sodium channel NavAb N49K/L176G mutant in sodium ion condition Deposited 2022-10-26	Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric	Chain A 1–267（267 aa）	Mutation：N49K, L176G	LMT DODECYL-BETA-D-MALTOSIDE × 8 1N7 CHAPSO × 4 PX4 1,2-DIMYRISTOYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 32 NA SODIUM ION × 8	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；9%-11% PEG MME 2000, 100 mM sodium chloride, 100 mM magnesium nitrate, 25 mM cadmium nitrate, 100 mM Tris-HCl, pH 8.4	Resolution 3.50 Å R-free 0.297
8HA2 Crystal structure of voltage-gated sodium channel NavAb N49K/L176G mutant in calcium ion condition Deposited 2022-10-26	Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric	Chain A 1–267（267 aa）	Mutation：N49K, L176G	LMT DODECYL-BETA-D-MALTOSIDE × 8 1N7 CHAPSO × 4 PX4 1,2-DIMYRISTOYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 32 CA CALCIUM ION × 8	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；9%-11% PEG MME 2000, 100 mM sodium chloride, 100 mM magnesium nitrate, 25mM cadmium nitrate, 100 mM Tris-HCl, pH 8.4	Resolution 3.30 Å R-free 0.281
9UC1 Crystal structure of voltage-gated sodium channel NavAb N49K/S178T mutant Deposited 2025-04-03	Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric	Chain A 1–267（267 aa）	Mutation：N49K, S178T	CA CALCIUM ION × 12 1N7 CHAPSO × 4 LMT DODECYL-BETA-D-MALTOSIDE × 4 PX4 1,2-DIMYRISTOYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 32	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.4；293 K；9%-11% PEG MME 2000, 100mM sodium chloride, 100mM magnesium nitrate, 25mM cadmium nitrate, 100mM Tris-HCl, pH 8.4	Resolution 2.50 Å R-free 0.274
9UC2 Crystal structure of voltage-gated sodium channel NavAb N49K/S178A mutant Deposited 2025-04-03	Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric	Chain A 1–267（267 aa）	Mutation：N49K,S178A	CA CALCIUM ION × 12 1N7 CHAPSO × 4 LMT DODECYL-BETA-D-MALTOSIDE × 4 PX4 1,2-DIMYRISTOYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 32	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.4；293 K；9%-11% PEG MME 2000, 100mM sodium chloride, 100mM magnesium nitrate, 25mM cadmium nitrate, 100mM Tris-HCl, pH 8.4	Resolution 2.80 Å R-free 0.294
9UC3 Crystal structure of voltage-gated sodium channel NavAb N49K/S178G mutant Deposited 2025-04-03	Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric	Chain A 1–267（267 aa）	Mutation：N49K,S178G	CA CALCIUM ION × 12 1N7 CHAPSO × 4 LMT DODECYL-BETA-D-MALTOSIDE × 4 PX4 1,2-DIMYRISTOYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 32	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.4；293 K；9%-11% PEG MME 2000, 100mM sodium chloride, 100mM magnesium nitrate, 25mM cadmium nitrate, 100mM Tris-HCl, pH 8.4	Resolution 2.90 Å R-free 0.299
9UC4 Crystal structure of voltage-gated sodium channel NavAb N49K/S178T/T206A mutant Deposited 2025-04-03	Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric	Chain A 1–267（267 aa）	Mutation：N49K, S178T, T206A	CA CALCIUM ION × 12 1N7 CHAPSO × 4 LMT DODECYL-BETA-D-MALTOSIDE × 4 PX4 1,2-DIMYRISTOYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 32	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；9%-11% PEG MME 2000, 100mM sodium chloride, 100mM magnesium nitrate, 25mM cadmium nitrate, 100mM Tris-HCl, pH 8.4	Resolution 3.40 Å R-free 0.312
9VDQ Crystal structure of voltage-gated sodium channel NavAb N49K mutant Deposited 2025-06-09	Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric	Chain A 1–267（267 aa）	Mutation：N49K	NA SODIUM ION × 12 1N7 CHAPSO × 4 PX4 1,2-DIMYRISTOYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 32	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；9%-11% PEG MME 2000, 100mM sodium chloride, 100mM magnesium nitrate, 25mM cadmium nitrate, 100mM Tris-HCl, pH 8.4	Resolution 3.10 Å R-free 0.289
9VDR Crystal structure of voltage-gated sodium channel NavAb N49K/L176F mutant Deposited 2025-06-09	Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric	Chain A 1–267（267 aa）	Mutation：N49K/L176F	NA SODIUM ION × 8 1N7 CHAPSO × 4 LMT DODECYL-BETA-D-MALTOSIDE × 4 PX4 1,2-DIMYRISTOYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 12	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；9%-11% PEG MME 2000, 100 mM sodium chloride, 100mM magnesium nitrate, 25mM cadmium nitrate, 100mM Tris-HCl, pH 8.4	Resolution 3.70 Å R-free 0.327
9VDS Crystal structure of voltage-gated sodium channel NavAb N49K/L176W mutant Deposited 2025-06-09	Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric	Chain A 1–267（267 aa）	Mutation：N49K/L176W	NA SODIUM ION × 4 1N7 CHAPSO × 4 LMT DODECYL-BETA-D-MALTOSIDE × 4	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；9%-11% PEG MME 2000, 100mM sodium chloride, 100mM magnesium nitrate, 25mM cadmium nitrate, 100mM Tris-HCl, pH 8.4	Resolution 3.70 Å R-free 0.339
9VDT Crystal structure of voltage-gated sodium channel NavAb N49K DC230 mutant Deposited 2025-06-09	Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric	Chain A 1–267（267 aa）	Mutation：N49K	NA SODIUM ION × 8 1N7 CHAPSO × 4 LMT DODECYL-BETA-D-MALTOSIDE × 4 PX4 1,2-DIMYRISTOYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 32	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；7%-9% PEG 6000, 100mM sodium chloride, 100mM magnesium nitrate, 100mM cadmium chloride, 10mM copper chloride, 100mM Tris-HCl, pH 8.4	Resolution 3.50 Å R-free 0.344
9VDU Crystal structure of voltage-gated sodium channel NavAb N49K/L176F DC230 mutant Deposited 2025-06-09	Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric	Chain A 1–230（230 aa）	Mutation：N49K/L176F	NA SODIUM ION × 8 1N7 CHAPSO × 4 PX4 1,2-DIMYRISTOYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 28	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；7%-9% PEG 6000, 100mM sodium chloride, 100mM magnesium nitrate, 100mM cadmium chloride, 10mM copper chloride, 100mM Tris-HCl, pH 8.4	Resolution 3.40 Å R-free 0.304

71 other PDB entries and 73 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence

UniProt name	A8EVM5_ARCB4
Isoform	—
PDB entities	1
Chains and sequence ranges	Author chain A； PDBConstruct 19–244； UniProt 1–226

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

SAXS scattering curve SAXS Profile

P(r) Distance Distribution P(r) Distribution

Download Download SAXS (.dat) P(r) (.pr.out) CIF

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2. Structure Basics 2. Structure Basics

Entry ID entry_id	5vb8
Deposition date deposition_date	2017-03-28
Structure title title	Crystal structure of the NavAb voltage-gated sodium channel in an open state
Keywords keywords	Voltage-gated sodium channel, TRANSPORT PROTEIN; TRANSPORT PROTEIN
Experimental Method method	X-RAY DIFFRACTION

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3. SAXS Parameters (CRYSOL theoretical calculation) 3. SAXS Parameters (CRYSOL)

Radius of gyration Rg (Guinier) rg_guinier	26.38 Å
Radius of gyration Rg (electron density) rg_electron	25.73 Å
Forward intensity I(0) i0	8195250.00
Molecular weight molecular_weight	25259.0 kDa
Excluded volume excluded_volume	33031 Å³
Envelope volume envelope_volume	44012 Å³
Hydration-shell volume shell_volume	15112 Å³
Envelope diameter envelope_diameter	97.0 Å
Shell Rg shell_rg	31.43 Å
Envelope Rg envelope_rg	25.14 Å
Shape Rg shape_rg	25.68 Å
Total Rg total_rg	26.73 Å
Total atoms total_atoms	3542
Residues n_residues	215
Spherical-harmonic order n_harmonics	20
q range q_range	— – 0.5000 Å⁻¹
Data points n_points	101
Shell type shell_type	directional
Solvent electron density solvent_density	0.3340 e/Å³
Shell contrast contrast_shell	0.0300 e/Å³
CRYSOL version crysol_version	4.1.3

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4. P(r) Distance Distribution (GNOM inversion) 4. P(r) Analysis (GNOM)

Maximum dimension Dmax dmax	96.0 Å
Rg (real space) rg_real	26.57 Å
Rg uncertainty (real space) rg_real_error	1.28 Å
I(0) (real space) i0_real	8.1950e+06
I(0) uncertainty (real space) i0_real_error	1.4470e+05
Rg (reciprocal space) rg_reciprocal	26.51 Å
I(0) (reciprocal space) i0_reciprocal	8195000.0000
Solution quality estimate total_estimate	0.7356
Solution quality rating solution_quality	REASONABLE a REASONABLE solution
P(r) peaks n_peaks	3
Primary peak position r_peak_primary	17.6 Å
Skewness Skewness skewness	0.289
Kurtosis Kurtosis kurtosis	-0.632
Angular range angular_range	— – 0.3000 Å⁻¹
Current regularization parameter α current_alpha	0.0000
Highest regularization parameter α highest_alpha	754100.0000
Real-space data points n_real_points	61
GNOM version gnom_version	4.1.3
Quality Criteria quality_criteria	AN1: 0.000; Oscil: 0.415; Stabil: 0.999; Sysdev: 1.000; Positv: 1.000; Valcen: 0.321; Smooth: 0.997

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5. Crystallography and Experiment 5. Crystallography & Experiment

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6. Entities and Polymers Entities & Polymers (5)

Entity 1 Entity 1 polymer

Description description	Ion transport protein
Molecular weight formula_weight	28202.6 kDa
Source method src_method	man
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	A
Atom count atom_count	3554

MDYKDDDDKGSLVPRGSHMYLRITNIVESSFFTKFIIYLIVLNGITMGLETSKTFMQSFGVYTTLFNQIVITIFTIEIILRIYVHRISFFKDPWSLFDFFVVAISLVPTSSGFEILRVLRVLRLFRLVTAVPQMRKIVSALISVIPGMLSVIALMTLFFYIFAIMATQLFGERFPEWFGTLGESFYTLFQVMTLESWSMGIVRPLMEVYPYAWVFFIPFIFVVTFVMINLVVAICVDAMAILNQ

来源 Source

来源生物 organism	Arcobacter butzleri
NCBI Class编号 ncbi_taxonomy_id	28197
宿主生物 host_organism	Trichoplusia ni

Entity 2 Entity 2 non-polymer

Description description	SODIUM ION
Molecular weight formula_weight	23.0 kDa
Source method src_method	syn
Copy count entity_count	1

Entity 3 Entity 3 non-polymer

Description description	1,2-DIMYRISTOYL-SN-GLYCERO-3-PHOSPHOCHOLINE
Molecular weight formula_weight	678.9 kDa
Source method src_method	syn
Copy count entity_count	4

Entity 4 Entity 4 non-polymer

Description description	SULFATE ION
Molecular weight formula_weight	96.1 kDa
Source method src_method	syn
Copy count entity_count	1

Entity 5 Entity 5 water

Description description	water
Molecular weight formula_weight	18.0 kDa
Source method src_method	nat
Copy count entity_count	12

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7. Fold Classification (SCOP + CATH) 4 domains

SCOP 2.08 (2 domains)

Domain ID domain_id	d5vb8a1
Class class	f — Membrane and cell surface proteins and peptides
Fold Fold fold	f.14 — Gated ion channels
Superfamily Superfamily superfamily	f.14.1 — Voltage-gated ion channels
Family Family family	f.14.1.2 — Voltage-gated Na/Ca cation channels

Domain ID domain_id	d5vb8a2
Class class	l — Artifacts
Fold Fold fold	l.1 — Tags
Superfamily Superfamily superfamily	l.1.1 — Tags
Family Family family	l.1.1.1 — Tags

CATH v4.4 (2 domains)

Domain ID domain_id	5vb8A01
Class class	1 — Mainly Alpha
Architecture architecture	20 — Up-down Bundle
Topology topology	120 — Four Helix Bundle (Hemerythrin (Met), subunit A)
Homologous superfamily homologous superfamily	350 — Voltage-gated potassium channels. Chain C

Domain ID domain_id	5vb8A02
Class class	1 — Mainly Alpha
Architecture architecture	10 — Orthogonal Bundle
Topology topology	287 — Helix Hairpins
Homologous superfamily homologous superfamily	70 —

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8. Citations (1)

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Unit-cell length a length_a	125.302 Å
Unit-cell length b length_b	125.302 Å
Unit-cell length c length_c	192.424 Å
Unit-cell angle α angle_alpha	90.00 °
Unit-cell angle β angle_beta	90.00 °
Unit-cell angle γ angle_gamma	90.00 °
Molecules per unit cell Z z_pdb	16

Space group space_group	I 4 2 2
Space-group number int_tables_number	97
Crystal system crystal_system	—

Resolution resolution	2.850 Å
Low-resolution limit resolution_low	29.530 Å
R factor r_factor	0.2295
R_work r_work	0.2282
R_free r_free	0.2536

fit	/app/data/saxs_computation/vb/5vb8/5vb8.fit
pddf	/app/data/pr_computation/vb/5vb8/5vb8.pr.out
profile	/app/data/saxs_computation/vb/5vb8/5vb8.dat