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7wpr

VWF D'D3 dimer complexed with D1D2 at 4.39 angstron resolution(VWF tube)

Method: ELECTRON MICROSCOPY Dmax: 302.8 Å Quality: GOOD
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1. Protein Identity and Related Structures Protein Identity & Related Structures

von Willebrand antigen 2

Homo sapiens

UniProt P04275

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein homooligomer Homooligomer Protein × 32 PDB declaration: 32-meric（32） Consistent with protein copy count Chain A； UniProt 23–763 Chain B； UniProt 23–763 Chain C； UniProt 764–1241 Chain D； UniProt 764–1241 Chain E； UniProt 23–763 Chain F； UniProt 23–763 Chain G； UniProt 23–763 Chain H； UniProt 23–763 Chain I； UniProt 23–763 Chain J； UniProt 23–763 Chain K； UniProt 23–763 Chain L； UniProt 23–763 Chain M； UniProt 23–763 Chain N； UniProt 23–763 Chain O； UniProt 23–763 Chain P； UniProt 23–763 Chain Q； UniProt 23–763 Chain R； UniProt 23–763 Chain S； UniProt 764–1241 Chain T； UniProt 764–1241 Chain U； UniProt 764–1241 Chain V； UniProt 764–1241 Chain W； UniProt 764–1241 Chain X； UniProt 764–1241 Chain Y； UniProt 764–1241 Chain Z； UniProt 764–1241 Chain a； UniProt 764–1241 Chain b； UniProt 764–1241 Chain c； UniProt 764–1241 Chain d； UniProt 764–1241 Chain e； UniProt 764–1241 Chain f； UniProt 764–1241 Fragment：D1D2 domain Fragment：;D'D3 domain ; NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 42 CA CALCIUM ION × 56 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.4 cryo-EM vitrification conditions：Cryogen ETHANE Resolution 4.39 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 7WPR Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
1AO3 A3 DOMAIN OF VON WILLEBRAND FACTOR Deposited 1997-07-16 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 1686–1872（187 aa） Fragment：A3 DOMAIN
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions pH 8.5；0.1M TRIS PH 8.5, 200MM MGCL2, 24% PEG 8K
Resolution 2.20 Å R-free 0.215
1AO3 A3 DOMAIN OF VON WILLEBRAND FACTOR Deposited 1997-07-16 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain B 1686–1872（187 aa） Fragment：A3 DOMAIN
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions pH 8.5；0.1M TRIS PH 8.5, 200MM MGCL2, 24% PEG 8K
Resolution 2.20 Å R-free 0.215
1ATZ HUMAN VON WILLEBRAND FACTOR A3 DOMAIN Deposited 1997-08-15 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 1685–1873（189 aa） Fragment：A3-DOMAIN
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions pH 8.5；24% PEG 3000, 200 MM SODIUM ACETATE, 100 MM TRIS/HCL PH 8.5
Resolution 1.80 Å R-free 0.240
1ATZ HUMAN VON WILLEBRAND FACTOR A3 DOMAIN Deposited 1997-08-15 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain B 1685–1873（189 aa） Fragment：A3-DOMAIN
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions pH 8.5；24% PEG 3000, 200 MM SODIUM ACETATE, 100 MM TRIS/HCL PH 8.5
Resolution 1.80 Å R-free 0.240
1AUQ A1 DOMAIN OF VON WILLEBRAND FACTOR Deposited 1997-09-01 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 1261–1468（208 aa） Fragment：A1
Not recorded CD CADMIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions pH 8.5；0.1M TRIS PH 8.5, 5MM CDCL2, 5% PEG 8K
Resolution 2.30 Å R-free 0.248
1FE8 CRYSTAL STRUCTURE OF THE VON WILLEBRAND FACTOR A3 DOMAIN IN COMPLEX WITH A FAB FRAGMENT OF IGG RU5 THAT INHIBITS COLLAGEN BINDING Deposited 2000-07-21 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 3 PDB declaration: trimeric
Chain A 1683–1874（192 aa） Fragment：COLLAGEN BINDING DOMAIN A3
Non-standard monomer：Yes (specific site not provided by mmCIF) NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 1 CAC CACODYLATE ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 5.5；277 K；iso-propanol, MPD, cacodylate, sodium chloride, Tris , pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 2.03 Å R-free 0.264
1FE8 CRYSTAL STRUCTURE OF THE VON WILLEBRAND FACTOR A3 DOMAIN IN COMPLEX WITH A FAB FRAGMENT OF IGG RU5 THAT INHIBITS COLLAGEN BINDING Deposited 2000-07-21 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Other combination Heteromer；Protein × 3 PDB declaration: trimeric
Chain B 1683–1874（192 aa） Fragment：COLLAGEN BINDING DOMAIN A3
Non-standard monomer：Yes (specific site not provided by mmCIF) NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 5.5；277 K；iso-propanol, MPD, cacodylate, sodium chloride, Tris , pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 2.03 Å R-free 0.264
1FE8 CRYSTAL STRUCTURE OF THE VON WILLEBRAND FACTOR A3 DOMAIN IN COMPLEX WITH A FAB FRAGMENT OF IGG RU5 THAT INHIBITS COLLAGEN BINDING Deposited 2000-07-21 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 3 Other combination Heteromer；Protein × 3 PDB declaration: trimeric
Chain C 1683–1874（192 aa） Fragment：COLLAGEN BINDING DOMAIN A3
Non-standard monomer：Yes (specific site not provided by mmCIF) No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 5.5；277 K；iso-propanol, MPD, cacodylate, sodium chloride, Tris , pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 2.03 Å R-free 0.264
1FE8 CRYSTAL STRUCTURE OF THE VON WILLEBRAND FACTOR A3 DOMAIN IN COMPLEX WITH A FAB FRAGMENT OF IGG RU5 THAT INHIBITS COLLAGEN BINDING Deposited 2000-07-21 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 4 Other combination Heteromer；Protein × 18 PDB declaration: octadecameric
Chain A 1683–1874（192 aa） Fragment：COLLAGEN BINDING DOMAIN A3
Chain B 1683–1874（192 aa） Fragment：COLLAGEN BINDING DOMAIN A3
Chain C 1683–1874（192 aa） Fragment：COLLAGEN BINDING DOMAIN A3
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 4 CAC CACODYLATE ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 5.5；277 K；iso-propanol, MPD, cacodylate, sodium chloride, Tris , pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 2.03 Å R-free 0.264
1FNS CRYSTAL STRUCTURE OF THE VON WILLEBRAND FACTOR (VWF) A1 DOMAIN I546V MUTANT IN COMPLEX WITH THE FUNCTION BLOCKING FAB NMC4 Deposited 2000-08-23 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain A 1271–1465（195 aa） Fragment：A1 DOMAIN RESIDUES 507 - 702, OR GLYCOPROTEIN IBA (A\:ALPHA) BINDING DOMAIN
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION mmCIF provides none of the parsed conditions Resolution 2.00 Å R-free 0.207
1IJB The von Willebrand Factor mutant (I546V) A1 domain Deposited 2001-04-25 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 1263–1464（202 aa） Fragment：A1 domain
Mutation：I546V No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 5.6；293 K；PEG 4000, sodium citrate, ammonium acetate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K
Resolution 1.80 Å R-free 0.208
1IJK The von Willebrand Factor mutant (I546V) A1 domain-botrocetin Complex Deposited 2001-04-26 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain A 1263–1464（202 aa） Fragment：A1 domain
Mutation：I546V No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 5.6；293 K；PEG 4000, sodium citrate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K
Resolution 2.60 Å R-free 0.279
1M10 Crystal structure of the complex of Glycoprotein Ib alpha and the von Willebrand Factor A1 Domain Deposited 2002-06-16 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1261–1468（208 aa） Fragment：A1 domain
Mutation：R543Q No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 5.5；277 K；PEG 3000, sodium chloride, MES, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 3.10 Å R-free 0.296
1OAK CRYSTAL STRUCTURE OF THE VON WILLEBRAND FACTOR (VWF) A1 DOMAIN IN COMPLEX WITH THE FUNCTION BLOCKING NMC-4 FAB Deposited 1997-12-18 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain A 1271–1465（195 aa） Fragment：A1 DOMAIN RESIDUES 507 - 702, OR GLYCOPROTEIN IBA (A\:ALPHA) BINDING DOMAIN
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions pH 8；pH 8
Resolution 2.20 Å R-free 0.274
1SQ0 Crystal Structure of the Complex of the Wild-type Von Willebrand Factor A1 domain and Glycoprotein Ib alpha at 2.6 Angstrom Resolution Deposited 2004-03-17 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1259–1472（214 aa） Fragment：A1
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8；291 K；PEG-3350; potassium thiocyanate, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K
Resolution 2.60 Å R-free 0.239
1U0N The ternary von Willebrand Factor A1-glycoprotein Ibalpha-botrocetin complex Deposited 2004-07-13 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric
Chain A 1261–1468（208 aa） Fragment：VWFA 1
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.5；277 K；Sodium citrate, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K
Resolution 2.95 Å R-free 0.276
1UEX Crystal structure of von Willebrand Factor A1 domain complexed with snake venom bitiscetin Deposited 2003-05-22 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain C 1260–1468（209 aa） Fragment：A1 domain
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.8；283 K；10% PEG 6000, 2% PEG-MME 550, 5% MPD, pH 7.8, VAPOR DIFFUSION, SITTING DROP, temperature 283K
Resolution 2.85 Å R-free 0.276
2ADF Crystal Structure and Paratope Determination of 82D6A3, an Antithrombotic Antibody Directed Against the von Willebrand factor A3-Domain Deposited 2005-07-20 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain A 1683–1874（192 aa） Fragment：A3 domain
Not recorded ACY ACETIC ACID × 1 SO4 SULFATE ION × 2 GOL GLYCEROL × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 4.6；293 K；PEG 8000, ammonium sulphate, acetate, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K
Resolution 1.90 Å R-free 0.220
2MHP Solution structure of the major factor VIII binding region on von Willebrand factor Deposited 2013-12-02 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 766–864（99 aa） Fragment：;domains TIL' and E', UNP residues 766-864 ;
Not recorded No recorded non-water small molecule SOLUTION NMR
NMR measurement conditions pH 7.4；298 K；Pressure ambient
NMR sample composition 300 mM [U-13C; U-15N] VWF TIL'E'-1, 20 mM sodium phosphate-2, 100 mM sodium chloride-3, 90% H2O/10% D2O | 90% H2O/10% D2O
NMR sample composition 300 mM [U-10% 13C] VWF TIL'E'-4, 20 mM sodium phosphate-5, 100 mM sodium chloride-6, 90% H2O/10% D2O | 90% H2O/10% D2O
NMR sample composition 300 mM [U-15N] VWF TIL'E'-7, 20 mM sodium phosphate-8, 100 mM sodium chloride-9, 90% H2O/10% D2O | 90% H2O/10% D2O
NMR sample composition 300 mM [U-13C; U-15N] VWF TIL'E'-10, 20 mM sodium phosphate-11, 100 mM sodium chloride-12, 90% H2O/10% D2O | 90% H2O/10% D2O
NMR sample composition 300 mM [U-15N] VWF TIL'E'-13, 20 mM sodium phosphate-14, 100 mM sodium chloride-15, 90% H2O/10% D2O | 90% H2O/10% D2O
NMR sample composition 300 mM [U-13C; U-15N] VWF TIL'E'-16, 20 mM sodium phosphate-17, 100 mM sodium chloride-18, 90% H2O/10% D2O | 90% H2O/10% D2O
NMR sample composition 300 mM [U-13C; U-15N] VWF TIL'E'-19, 20 mM sodium phosphate-20, 100 mM sodium chloride-21, 5 mg/mL Pf1 phage-22, 90% H2O/10% D2O | 90% H2O/10% D2O
NMR sample composition 300 mM [U-13C; U-15N] VWF TIL'E'-23, 20 mM sodium phosphate-24, 100 mM sodium chloride-25, 2 % w/v pentaethylene glycol dodecyl ether-26, 50 mM n-hexanol-27, 90% H2O/10% D2O | 90% H2O/10% D2O
Resolution not provided
2MHQ Solution structure of the major factor VIII binding region on von Willebrand factor Deposited 2013-12-02 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 766–864（99 aa） Fragment：;Domains TIL' and E', UNP residues 766-864 ;
Not recorded No recorded non-water small molecule SOLUTION NMR
NMR measurement conditions pH 7.4；298 K；Pressure ambient
NMR sample composition 300 mM [U-13C; U-15N] VWF TIL'E'-1, 20 mM sodium phosphate-2, 100 mM sodium chloride-3, 90% H2O/10% D2O | 90% H2O/10% D2O
NMR sample composition 300 mM [U-10% 13C] VWF TIL'E'-4, 20 mM sodium phosphate-5, 100 mM sodium chloride-6, 90% H2O/10% D2O | 90% H2O/10% D2O
NMR sample composition 300 mM [U-15N] VWF TIL'E'-7, 20 mM sodium phosphate-8, 100 mM sodium chloride-9, 90% H2O/10% D2O | 90% H2O/10% D2O
NMR sample composition 300 mM [U-13C; U-15N] VWF TIL'E'-10, 20 mM sodium phosphate-11, 100 mM sodium chloride-12, 90% H2O/10% D2O | 90% H2O/10% D2O
NMR sample composition 300 mM [U-15N] VWF TIL'E'-13, 20 mM sodium phosphate-14, 100 mM sodium chloride-15, 90% H2O/10% D2O | 90% H2O/10% D2O
NMR sample composition 300 mM [U-13C; U-15N] VWF TIL'E'-16, 20 mM sodium phosphate-17, 100 mM sodium chloride-18, 90% H2O/10% D2O | 90% H2O/10% D2O
NMR sample composition 300 mM [U-13C; U-15N] VWF TIL'E'-19, 20 mM sodium phosphate-20, 100 mM sodium chloride-21, 5 mg/mL Pf1 phage-22, 90% H2O/10% D2O | 90% H2O/10% D2O
NMR sample composition 300 mM [U-13C; U-15N] VWF TIL'E'-23, 20 mM sodium phosphate-24, 100 mM sodium chloride-25, 2 % w/v pentaethylene glycol dodecyl ether-26, 50 mM n-hexanol-27, 90% H2O/10% D2O | 90% H2O/10% D2O
Resolution not provided
3GXB Crystal structure of VWF A2 domain Deposited 2009-04-02 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Monomer；Protein × 1 PDB declaration: monomeric
Chain A 1495–1671（177 aa） Fragment：ADAMTS13 cleavage domain, residues 1495-1671
Not recorded NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 1 X-RAY DIFFRACTION
X-ray crystallization conditions pH 7.2；277 K；0.1 M Bis-Tris pH 7.2, 0.2 M lithium sulfate, 25 % PEG3350, vapor diffusion, hanging drop, temperature 277K
Resolution 1.90 Å R-free 0.224
3GXB Crystal structure of VWF A2 domain Deposited 2009-04-02 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Other combination Monomer；Protein × 1 PDB declaration: monomeric
Chain B 1495–1671（177 aa） Fragment：ADAMTS13 cleavage domain, residues 1495-1671
Not recorded NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 1 SO4 SULFATE ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions pH 7.2；277 K；0.1 M Bis-Tris pH 7.2, 0.2 M lithium sulfate, 25 % PEG3350, vapor diffusion, hanging drop, temperature 277K
Resolution 1.90 Å R-free 0.224
3HXO Crystal Structure of Von Willebrand Factor (VWF) A1 Domain in Complex with DNA Aptamer ARC1172, an Inhibitor of VWF-Platelet Binding Deposited 2009-06-21 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein–DNA Monomer；Protein × 1 PDB declaration: dimeric
Chain A 1260–1468（209 aa） Fragment：von Willebrand factor (VWF) A1 Domain (residues 1260-1468)
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions pH 7.1；293 K；25% PEG3350, 0.16 M sodium fluoride, pH 7.1, EVAPORATION, temperature 293K
Resolution 2.40 Å R-free 0.274
3HXQ Crystal Structure of Von Willebrand Factor (VWF) A1 Domain in Complex with DNA Aptamer ARC1172, an Inhibitor of VWF-Platelet Binding Deposited 2009-06-21 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein–DNA Monomer；Protein × 1 PDB declaration: dimeric
Chain A 1260–1468（209 aa） Fragment：von Willebrand factor (VWF) A1 Domain (residues 1260-1468)
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions pH 4.6；293 K；0.2 M NH4Ac, 0.1 M NaAc, 30% PEG4000, pH 4.6, EVAPORATION, temperature 293K
Resolution 2.69 Å R-free 0.279
3PPV Crystal structure of an engineered VWF A2 domain (N1493C and C1670S) Deposited 2010-11-25 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 1488–1674（187 aa） Fragment：VWFA 2 domain, UNP residues 1488-1674
Mutation：N1493C, C1670S SO4 SULFATE ION × 1 CA CALCIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 5.2；293 K；0.1M sodium citrate, 1.6M (NH4)2SO4, pH 5.2, VAPOR DIFFUSION, HANGING DROP, temperature 293K
Resolution 1.90 Å R-free 0.231
3PPW Crystal structure of the D1596A mutant of an engineered VWF A2 domain (N1493C and C1670S) Deposited 2010-11-25 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 1488–1674（187 aa） Fragment：VWFA 2 domain, UNP residues 1488-1674
Mutation：N1493C, D1596A, C1670S NA SODIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 5.2；293 K；0.1M sodium citrate, 1.6M (NH4)2SO4, pH 5.2, VAPOR DIFFUSION, HANGING DROP, temperature 293K
Resolution 1.90 Å R-free 0.226
3PPX Crystal structure of the N1602A mutant of an engineered VWF A2 domain (N1493C and C1670S) Deposited 2010-11-25 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 1488–1674（187 aa） Fragment：VWF A2 domain, UNP residues 1488-1674
Mutation：N1493C, N1602A, C1670S NA SODIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 5.2；293 K；0.1M sodium citrate, 1.6M (NH4)2SO4, pH 5.2, VAPOR DIFFUSION, HANGING DROP, temperature 293K
Resolution 1.91 Å R-free 0.237
3PPY Crystal structure of the D1596A/N1602A double mutant of an engineered VWF A2 domain (N1493C and C1670S) Deposited 2010-11-25 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 1488–1674（187 aa） Fragment：VWF A2 domain, UNP residues 1488-1674
Mutation：N1493, D1596A, N1602A, C1670S NA SODIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 5.2；293 K；0.1M sodium citrate, 1.6M (NH4)2SO4, pH 5.2, VAPOR DIFFUSION, HANGING DROP, temperature 293K
Resolution 2.00 Å R-free 0.232
3ZQK Von Willebrand Factor A2 domain with calcium Deposited 2011-06-09 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 1478–1674（197 aa） Fragment：A2 DOMAIN, RESIDUES 1478-1674
Not recorded GOL GLYCEROL × 2 CA CALCIUM ION × 1 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 2 X-RAY DIFFRACTION
X-ray crystallization conditions PROTEIN BUFFER: 0.015 M HEPES PH 7.5, 0.02 M NACL, 0.001 M CACL2. RESERVOIR BUFFER: 0.1 M MMT (PH 4.0-5.5), 0.15 M (NH4)2SO4, 18-23% (W/V) PEG2000 MME.
Resolution 1.70 Å R-free 0.200
3ZQK Von Willebrand Factor A2 domain with calcium Deposited 2011-06-09 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain B 1478–1674（197 aa） Fragment：A2 DOMAIN, RESIDUES 1478-1674
Not recorded CA CALCIUM ION × 1 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 2 X-RAY DIFFRACTION
X-ray crystallization conditions PROTEIN BUFFER: 0.015 M HEPES PH 7.5, 0.02 M NACL, 0.001 M CACL2. RESERVOIR BUFFER: 0.1 M MMT (PH 4.0-5.5), 0.15 M (NH4)2SO4, 18-23% (W/V) PEG2000 MME.
Resolution 1.70 Å R-free 0.200
3ZQK Von Willebrand Factor A2 domain with calcium Deposited 2011-06-09 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain C 1478–1674（197 aa） Fragment：A2 DOMAIN, RESIDUES 1478-1674
Not recorded CA CALCIUM ION × 1 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 2 X-RAY DIFFRACTION
X-ray crystallization conditions PROTEIN BUFFER: 0.015 M HEPES PH 7.5, 0.02 M NACL, 0.001 M CACL2. RESERVOIR BUFFER: 0.1 M MMT (PH 4.0-5.5), 0.15 M (NH4)2SO4, 18-23% (W/V) PEG2000 MME.
Resolution 1.70 Å R-free 0.200
4C29 Crystal Structure of High-Affinity von Willebrand Factor A1 domain with Disulfide Mutation Deposited 2013-08-16 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 1264–1471（208 aa） Fragment：RESIDUES 1264-1471
Mutation：YES ACT ACETATE ION × 1 PEG DI(HYDROXYETHYL)ETHER × 2 CA CALCIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions pH 4.6；CRYSTALS OF A1/SS APPEARED IN DROPS CONTAINING 25% PEG3350 AND 0.2 M CALCIUM ACETATE. THESE CRYSTALS WERE CRUSHED AND USED FOR SEEDING. CRYSTALS GROWN IN 8 MG/ML PROTEIN, 15% PEG3350, AND 0.2 M CALCIUM ACETATE WERE HARVESTED, AND SOAKED IN THE SAME BUFFER CONTAINING 20% GLYCEROL FOR CRYOPROTECTION., pH 4.6
Resolution 2.20 Å R-free 0.235
4C29 Crystal Structure of High-Affinity von Willebrand Factor A1 domain with Disulfide Mutation Deposited 2013-08-16 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain B 1264–1471（208 aa） Fragment：RESIDUES 1264-1471
Mutation：YES PEG DI(HYDROXYETHYL)ETHER × 2 CA CALCIUM ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions pH 4.6；CRYSTALS OF A1/SS APPEARED IN DROPS CONTAINING 25% PEG3350 AND 0.2 M CALCIUM ACETATE. THESE CRYSTALS WERE CRUSHED AND USED FOR SEEDING. CRYSTALS GROWN IN 8 MG/ML PROTEIN, 15% PEG3350, AND 0.2 M CALCIUM ACETATE WERE HARVESTED, AND SOAKED IN THE SAME BUFFER CONTAINING 20% GLYCEROL FOR CRYOPROTECTION., pH 4.6
Resolution 2.20 Å R-free 0.235
4C2A Crystal Structure of High-Affinity von Willebrand Factor A1 domain with R1306Q and I1309V Mutations in Complex with High Affinity GPIb alpha Deposited 2013-08-16 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1264–1471（208 aa） Fragment：RESIDUES 1264-1471
Mutation：YES CAC CACODYLATE ION × 1 ACT ACETATE ION × 1 CA CALCIUM ION × 1 PEG DI(HYDROXYETHYL)ETHER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions pH 6.5；CRYSTALS OF A1/VWD2-GPIBALPA/VWD2 COMPLEX APPEARED IN 12% PEG 8000, 0.1 M SODIUM CACODYLATE PH 6.5, 0.1 M CALCIUM ACETATE, AND 0.4 MG/ML RISTOCETIN (SIGMA-ALDRICH). THESE CRYSTALS WERE CRUSHED AND USED FOR SEEDING. CRYSTALS GREW IN 10 MG/ML COMPLEX, 14% PEG8000, 0.2 M CALCIUM ACETATE, 0.1 M SODIUM CACODYLATE, PH 6.5, AND 0.4 MG/ML RISTOCETIN. NEXT WE SOAKED THESE CRYSTALS IN 14% PEG8000, 0.2 M CALCIUM ACETATE, 0.1 SODIUM CACODYLATE, PH 6.5, AND 4 MG/ML RISTOCETIN.
Resolution 2.08 Å R-free 0.189
4C2B Crystal Structure of High-Affinity von Willebrand Factor A1 domain with Disulfide Mutation in Complex with High Affinity GPIb alpha Deposited 2013-08-16 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1264–1471（208 aa） Fragment：1264-1468
Mutation：YES SO4 SULFATE ION × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 1 X-RAY DIFFRACTION
X-ray crystallization conditions pH 4.6；CRYSTALS OF A1/SS-GPIB/VWD2 COMPLEX APPEARED IN DROPS WITH 20% PEG 4000, 0.16 M AMMONIUM SULFATE, 0.08M SODIUM ACETATE, PH 4.6, AND 20% GLYCEROL. THESE CRYSTALS WERE CRUSHED AND USED FOR SEEDING CRYSTAL GROWTH IN 8 MG/ML COMPLEX, 15% PEG 4000, 0.16 M AMMONIUM SULFATE, 0.08 M SODIUM ACETATE, PH 4.6, AND 20% GLYCEROL. SINCE THESE CRYSTALS WERE FORMED IN BUFFER CONTAINING 20% GLYCEROL, NO ADDITIONAL CRYOPROTECTION WAS USED.
Resolution 2.80 Å R-free 0.286
4C2B Crystal Structure of High-Affinity von Willebrand Factor A1 domain with Disulfide Mutation in Complex with High Affinity GPIb alpha Deposited 2013-08-16 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain C 1264–1471（208 aa） Fragment：1264-1468
Mutation：YES SO4 SULFATE ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions pH 4.6；CRYSTALS OF A1/SS-GPIB/VWD2 COMPLEX APPEARED IN DROPS WITH 20% PEG 4000, 0.16 M AMMONIUM SULFATE, 0.08M SODIUM ACETATE, PH 4.6, AND 20% GLYCEROL. THESE CRYSTALS WERE CRUSHED AND USED FOR SEEDING CRYSTAL GROWTH IN 8 MG/ML COMPLEX, 15% PEG 4000, 0.16 M AMMONIUM SULFATE, 0.08 M SODIUM ACETATE, PH 4.6, AND 20% GLYCEROL. SINCE THESE CRYSTALS WERE FORMED IN BUFFER CONTAINING 20% GLYCEROL, NO ADDITIONAL CRYOPROTECTION WAS USED.
Resolution 2.80 Å R-free 0.286
4C2B Crystal Structure of High-Affinity von Willebrand Factor A1 domain with Disulfide Mutation in Complex with High Affinity GPIb alpha Deposited 2013-08-16 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 3 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain E 1264–1471（208 aa） Fragment：1264-1468
Mutation：YES SO4 SULFATE ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions pH 4.6；CRYSTALS OF A1/SS-GPIB/VWD2 COMPLEX APPEARED IN DROPS WITH 20% PEG 4000, 0.16 M AMMONIUM SULFATE, 0.08M SODIUM ACETATE, PH 4.6, AND 20% GLYCEROL. THESE CRYSTALS WERE CRUSHED AND USED FOR SEEDING CRYSTAL GROWTH IN 8 MG/ML COMPLEX, 15% PEG 4000, 0.16 M AMMONIUM SULFATE, 0.08 M SODIUM ACETATE, PH 4.6, AND 20% GLYCEROL. SINCE THESE CRYSTALS WERE FORMED IN BUFFER CONTAINING 20% GLYCEROL, NO ADDITIONAL CRYOPROTECTION WAS USED.
Resolution 2.80 Å R-free 0.286
4C2B Crystal Structure of High-Affinity von Willebrand Factor A1 domain with Disulfide Mutation in Complex with High Affinity GPIb alpha Deposited 2013-08-16 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 4 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain G 1264–1471（208 aa） Fragment：1264-1468
Mutation：YES SO4 SULFATE ION × 4 PEG DI(HYDROXYETHYL)ETHER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions pH 4.6；CRYSTALS OF A1/SS-GPIB/VWD2 COMPLEX APPEARED IN DROPS WITH 20% PEG 4000, 0.16 M AMMONIUM SULFATE, 0.08M SODIUM ACETATE, PH 4.6, AND 20% GLYCEROL. THESE CRYSTALS WERE CRUSHED AND USED FOR SEEDING CRYSTAL GROWTH IN 8 MG/ML COMPLEX, 15% PEG 4000, 0.16 M AMMONIUM SULFATE, 0.08 M SODIUM ACETATE, PH 4.6, AND 20% GLYCEROL. SINCE THESE CRYSTALS WERE FORMED IN BUFFER CONTAINING 20% GLYCEROL, NO ADDITIONAL CRYOPROTECTION WAS USED.
Resolution 2.80 Å R-free 0.286
4DMU Crystal structure of the von Willebrand factor A3 domain in complex with a collagen III derived triple-helical peptide Deposited 2012-02-08 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain B 1683–1874（192 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.5；277 K；100 mM cacodylate, pH 6.5, 1.20 M (NH4)2SO4, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 2.80 Å R-free 0.253
4DMU Crystal structure of the von Willebrand factor A3 domain in complex with a collagen III derived triple-helical peptide Deposited 2012-02-08 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain D 1683–1874（192 aa）
Not recorded SO4 SULFATE ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.5；277 K；100 mM cacodylate, pH 6.5, 1.20 M (NH4)2SO4, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 2.80 Å R-free 0.253
4DMU Crystal structure of the von Willebrand factor A3 domain in complex with a collagen III derived triple-helical peptide Deposited 2012-02-08 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 3 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain F 1683–1874（192 aa）
Not recorded SO4 SULFATE ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.5；277 K；100 mM cacodylate, pH 6.5, 1.20 M (NH4)2SO4, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 2.80 Å R-free 0.253
4DMU Crystal structure of the von Willebrand factor A3 domain in complex with a collagen III derived triple-helical peptide Deposited 2012-02-08 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 4 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain H 1683–1874（192 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.5；277 K；100 mM cacodylate, pH 6.5, 1.20 M (NH4)2SO4, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 2.80 Å R-free 0.253
4DMU Crystal structure of the von Willebrand factor A3 domain in complex with a collagen III derived triple-helical peptide Deposited 2012-02-08 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 5 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain J 1683–1874（192 aa）
Not recorded SO4 SULFATE ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.5；277 K；100 mM cacodylate, pH 6.5, 1.20 M (NH4)2SO4, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 2.80 Å R-free 0.253
4DMU Crystal structure of the von Willebrand factor A3 domain in complex with a collagen III derived triple-helical peptide Deposited 2012-02-08 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 6 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain L 1683–1874（192 aa）
Not recorded SO4 SULFATE ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.5；277 K；100 mM cacodylate, pH 6.5, 1.20 M (NH4)2SO4, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 2.80 Å R-free 0.253
4NT5 Crystal structure of human von Willebrand factor CTCK domain Deposited 2013-12-01 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 2721–2813（93 aa） Fragment：C-terminal cystine knot domain, unp residues 2721-2813
Not recorded NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 2 ZN ZINC ION × 2 SO4 SULFATE ION × 10 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8.3；293 K；1.3 M Lithium Sulfate, 0.1 M Bicine, pH 8.3, VAPOR DIFFUSION, HANGING DROP, temperature 293K
Resolution 3.28 Å R-free 0.248
5BV8 G1324S mutation in von Willebrand Factor A1 domain Deposited 2015-06-04 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 1238–1471（234 aa） Fragment：A1 domain (UNP residues 1238-1471)
Mutation：G1324S EDO 1,2-ETHANEDIOL × 1 CL CHLORIDE ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 10.5；298 K；30% PEG 400, 100 mM CAPS/Sodium hydroxide
Resolution 1.59 Å R-free 0.181
6FWN Structure and dynamics of the platelet integrin-binding C4 domain of von Willebrand factor Deposited 2018-03-06 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 2497–2577（81 aa）
Not recorded No recorded non-water small molecule SOLUTION NMR
NMR measurement conditions pH 6.5；298 K；Ionic strength (raw mmCIF value) 0.04；Pressure 1
NMR sample composition 500 uM [U-13C; U-15N] von Willebrand Factor C4 domain, 20 mM sodium phosphate, 49.5 M H2O, 5.5 M D2O, 0.02 % w/v sodium azide, 90% H2O/10% D2O | 90% H2O/10% D2O
NMR sample composition 500 uM [U-13C; U-15N] von Willebrand Factor C4 domain, 20 mM sodium phosphate, 55 M D2O, 5 mM H2O, 0.02 % w/v sodium azide, 100% D2O | 100% D2O
Resolution not provided
6N29 Crystal structure of monomeric von Willebrand Factor D`D3 assembly Deposited 2018-11-12 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 764–1244（481 aa）
Mutation：Q852R, C1009A, C1142A NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 3 CA CALCIUM ION × 2 PEG DI(HYDROXYETHYL)ETHER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；297 K；5.5% (w/v) PEG8000, 10% (w/v) PEG1000, 0.2 M calcium acetate and 0.1 M Tris-acetate, pH 8.5
Resolution 2.50 Å R-free 0.241
6N29 Crystal structure of monomeric von Willebrand Factor D`D3 assembly Deposited 2018-11-12 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain B 764–1244（481 aa）
Mutation：Q852R, C1009A, C1142A NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 2 CA CALCIUM ION × 1 PEG DI(HYDROXYETHYL)ETHER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；297 K；5.5% (w/v) PEG8000, 10% (w/v) PEG1000, 0.2 M calcium acetate and 0.1 M Tris-acetate, pH 8.5
Resolution 2.50 Å R-free 0.241
7EOW High-resolution structure of vWF A1 domain in complex with caplacizumab, the first nanobody-based medicine Deposited 2021-04-23 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1261–1468（208 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8；293.15 K；20 mM Tris-HCl pH 8 150 mM NaCl 10% glycerol 40% glycerol ethoxylate
Resolution 1.60 Å R-free 0.198
7F49 von Willebrand factor (VWF) A1 domain with BT-100 aptamer RNA Deposited 2021-06-18 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein–DNA Monomer；Protein × 1 PDB declaration: dimeric
Chain A 1260–1468（209 aa）
Not recorded GOL GLYCEROL × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；291 K；0.1M Sodium Acetate pH 4.6, 18% w/v PEG 4000
Resolution 2.09 Å R-free 0.239
7P4N NMR solution structure of the C6 domain of von Willebrand Factor Deposited 2021-07-12 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 2647–2720（74 aa）
Mutation：G2705R No recorded non-water small molecule SOLUTION NMR
NMR measurement conditions pH 6.5；298 K；Ionic strength (raw mmCIF value) 20；Pressure 1.013
NMR sample composition 300 uM [U-13C; U-15N] von Willebrand Factor C6 domain, 20 mM None sodium phosphate, 90% H2O/10% D2O | 90% H2O/10% D2O
Resolution not provided
7PMV VWF Tubules of D1D2D'D3 domains Deposited 2021-09-02 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 1–1241（1241 aa）
Chain B 1–1241（1241 aa）
Chain D 1–1241（1241 aa）
Chain E 1–1241（1241 aa）
Not recorded NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 16 CA CALCIUM ION × 12 ELECTRON MICROSCOPY
cryo-EM buffer pH 6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.70 Å
7PNF VWF Tubules of D1D2D'D3 domains Deposited 2021-09-06 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Homooligomer；Protein × 36 PDB declaration: 36-meric
Chain B 1–1241（1241 aa）
Chain D 1–1241（1241 aa）
Not recorded NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 144 CA CALCIUM ION × 108 ELECTRON MICROSCOPY
cryo-EM buffer pH 6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.35 Å
7WN3 Cryo-EM structure of VWF D'D3 dimer (2M mutant) complexed with D1D2 at 3.29 angstron resolution (2 units) Deposited 2022-01-17 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 8 PDB declaration: octameric
Chain A 23–763（741 aa） Fragment：D1D2 domain
Chain B 23–763（741 aa） Fragment：D1D2 domain
Chain C 764–1241（478 aa） Fragment：;D'D3 domain ;
Chain D 764–1241（478 aa） Fragment：;D'D3 domain ;
Chain E 23–763（741 aa） Fragment：D1D2 domain
Chain F 764–1241（478 aa） Fragment：;D'D3 domain ;
Chain G 23–763（741 aa） Fragment：D1D2 domain
Chain H 764–1241（478 aa） Fragment：;D'D3 domain ;
Mutation：R1136M,E1143M Mutation：R1136M,E1143M Mutation：R1136M,E1143M Mutation：R1136M,E1143M CA CALCIUM ION × 16 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 20 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.29 Å
7WN4 Cryo-EM structure of VWF D'D3 dimer (wild type) complexed with D1D2 at 3.4 angstron resolution (1 unit) Deposited 2022-01-17 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain E 23–763（741 aa） Fragment：D1D2 domain
Chain F 764–1241（478 aa） Fragment：;D'D3 domain ;
Chain G 23–763（741 aa） Fragment：D1D2 domain
Chain H 764–1241（478 aa） Fragment：;D'D3 domain ;
Not recorded CA CALCIUM ION × 6 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
7WN6 Cryo-EM structure of VWF D'D3 dimer (R1136M/E1143M mutant) complexed with D1D2 at 3.29 angstron resolution (2 units) Deposited 2022-01-17 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 8 PDB declaration: octameric
Chain A 23–763（741 aa） Fragment：D1D2 domain
Chain B 23–763（741 aa） Fragment：D1D2 domain
Chain C 764–1241（478 aa） Fragment：;D'D3 domain ;
Chain D 764–1241（478 aa） Fragment：;D'D3 domain ;
Chain E 23–763（741 aa） Fragment：D1D2 domain
Chain F 764–1241（478 aa） Fragment：;D'D3 domain ;
Chain G 23–763（741 aa） Fragment：D1D2 domain
Chain H 764–1241（478 aa） Fragment：;D'D3 domain ;
Mutation：R1136M/E1143M Mutation：R1136M/E1143M Mutation：R1136M/E1143M Mutation：R1136M/E1143M CA CALCIUM ION × 16 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 20 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.29 Å
7WPP Cryo-EM structure of VWF D'D3 dimer complexed with D1D2 at 2.85 angstron resolution (1 unit) Deposited 2022-01-24 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain E 23–763（741 aa） Fragment：D1D2 domain
Chain F 764–1241（478 aa） Fragment：;D'D3 domain ;
Chain G 23–763（741 aa） Fragment：D1D2 domain
Chain H 764–1241（478 aa） Fragment：;D'D3 domain ;
Not recorded CA CALCIUM ION × 6 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 10 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.85 Å
7WPQ Cryo-EM structure of VWF D'D3 dimer complexed with D1D2 at 3.27 angstron resolution (2 units) Deposited 2022-01-24 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 8 PDB declaration: octameric
Chain A 23–763（741 aa） Fragment：D1D2 domain
Chain B 23–763（741 aa） Fragment：D1D2 domain
Chain C 764–1241（478 aa） Fragment：;D'D3 domain ;
Chain D 764–1241（478 aa） Fragment：;D'D3 domain ;
Chain E 23–763（741 aa） Fragment：D1D2 domain
Chain F 764–1241（478 aa） Fragment：;D'D3 domain ;
Chain G 23–763（741 aa） Fragment：D1D2 domain
Chain H 764–1241（478 aa） Fragment：;D'D3 domain ;
Not recorded NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 20 CA CALCIUM ION × 16 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.27 Å
7WPS Cryo-EM structure of VWF D'D3 dimer complexed with D1D2 at 4.3 angstron resolution (7 units) Deposited 2022-01-24 Different construct Different mutation/modification Different oligomeric state Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 28 PDB declaration: 28-meric
Chain A 23–763（741 aa） Fragment：D1D2 domain
Chain B 23–763（741 aa） Fragment：D1D2 domain
Chain C 764–1241（478 aa） Fragment：;D'D3 domain ;
Chain D 764–1241（478 aa） Fragment：;D'D3 domain ;
Chain E 23–763（741 aa） Fragment：D1D2 domain
Chain F 764–1241（478 aa） Fragment：;D'D3 domain ;
Chain G 23–763（741 aa） Fragment：D1D2 domain
Chain H 764–1241（478 aa） Fragment：;D'D3 domain ;
Chain I 23–763（741 aa） Fragment：D1D2 domain
Chain J 764–1241（478 aa） Fragment：;D'D3 domain ;
Chain K 23–763（741 aa） Fragment：D1D2 domain
Chain L 764–1241（478 aa） Fragment：;D'D3 domain ;
Chain M 23–763（741 aa） Fragment：D1D2 domain
Chain N 764–1241（478 aa） Fragment：;D'D3 domain ;
Chain O 23–763（741 aa） Fragment：D1D2 domain
Chain P 764–1241（478 aa） Fragment：;D'D3 domain ;
Chain Q 23–763（741 aa） Fragment：D1D2 domain
Chain R 764–1241（478 aa） Fragment：;D'D3 domain ;
Chain S 23–763（741 aa） Fragment：D1D2 domain
Chain T 764–1241（478 aa） Fragment：;D'D3 domain ;
Chain U 23–763（741 aa） Fragment：D1D2 domain
Chain V 764–1241（478 aa） Fragment：;D'D3 domain ;
Chain W 23–763（741 aa） Fragment：D1D2 domain
Chain X 764–1241（478 aa） Fragment：;D'D3 domain ;
Chain Y 23–763（741 aa） Fragment：D1D2 domain
Chain Z 764–1241（478 aa） Fragment：;D'D3 domain ;
Chain a 23–763（741 aa） Fragment：D1D2 domain
Chain b 764–1241（478 aa） Fragment：;D'D3 domain ;
Not recorded NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 42 CA CALCIUM ION × 56 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.32 Å
7WQT Cryo-EM structure of VWF D'D3 dimer complexed with D1D2 at 4.3 angstron resolution (VWF tube) Deposited 2022-01-26 Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 32 PDB declaration: 32-meric
Chain A 23–763（741 aa） Fragment：D1D2 domain
Chain B 23–763（741 aa） Fragment：D1D2 domain
Chain C 764–1241（478 aa） Fragment：;D'D3 domain ;
Chain D 764–1241（478 aa） Fragment：;D'D3 domain ;
Chain E 23–763（741 aa） Fragment：D1D2 domain
Chain F 23–763（741 aa） Fragment：D1D2 domain
Chain G 23–763（741 aa） Fragment：D1D2 domain
Chain H 23–763（741 aa） Fragment：D1D2 domain
Chain I 23–763（741 aa） Fragment：D1D2 domain
Chain J 23–763（741 aa） Fragment：D1D2 domain
Chain K 23–763（741 aa） Fragment：D1D2 domain
Chain L 23–763（741 aa） Fragment：D1D2 domain
Chain M 23–763（741 aa） Fragment：D1D2 domain
Chain N 23–763（741 aa） Fragment：D1D2 domain
Chain O 23–763（741 aa） Fragment：D1D2 domain
Chain P 23–763（741 aa） Fragment：D1D2 domain
Chain Q 23–763（741 aa） Fragment：D1D2 domain
Chain R 23–763（741 aa） Fragment：D1D2 domain
Chain S 764–1241（478 aa） Fragment：;D'D3 domain ;
Chain T 764–1241（478 aa） Fragment：;D'D3 domain ;
Chain U 764–1241（478 aa） Fragment：;D'D3 domain ;
Chain V 764–1241（478 aa） Fragment：;D'D3 domain ;
Chain W 764–1241（478 aa） Fragment：;D'D3 domain ;
Chain X 764–1241（478 aa） Fragment：;D'D3 domain ;
Chain Y 764–1241（478 aa） Fragment：;D'D3 domain ;
Chain Z 764–1241（478 aa） Fragment：;D'D3 domain ;
Chain a 764–1241（478 aa） Fragment：;D'D3 domain ;
Chain b 764–1241（478 aa） Fragment：;D'D3 domain ;
Chain c 764–1241（478 aa） Fragment：;D'D3 domain ;
Chain d 764–1241（478 aa） Fragment：;D'D3 domain ;
Chain e 764–1241（478 aa） Fragment：;D'D3 domain ;
Chain f 764–1241（478 aa） Fragment：;D'D3 domain ;
Not recorded NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 42 CA CALCIUM ION × 56 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.30 Å
7ZWH VWF Tubules of D1D2 and D'D3A1 domains Deposited 2022-05-19 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain D 1–1197（1197 aa）
Chain E 1–1197（1197 aa）
Chain G 1265–1463（199 aa）
Chain H 1265–1463（199 aa）
Not recorded NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 8 CA CALCIUM ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 5.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
8D3C VWF tubule derived from monomeric D1-A1 Deposited 2022-06-01 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 16 PDB declaration: hexadecameric
Chain A 1–1464（1464 aa）
Chain B 1–1464（1464 aa）
Chain C 1–1464（1464 aa）
Chain D 1–1464（1464 aa）
Chain E 1–1464（1464 aa）
Chain F 1–1464（1464 aa）
Chain G 1–1464（1464 aa）
Chain H 1–1464（1464 aa）
Chain I 1–1464（1464 aa）
Chain J 1–1464（1464 aa）
Chain K 1–1464（1464 aa）
Chain L 1–1464（1464 aa）
Chain M 1–1464（1464 aa）
Chain N 1–1464（1464 aa）
Chain O 1–1464（1464 aa）
Chain P 1–1464（1464 aa）
Mutation：WT VWF Furin cleavage site mutated (760RSKR763->760ASA) Mutation：WT VWF Furin cleavage site mutated (760RSKR763->760ASA) Mutation：WT VWF Furin cleavage site mutated (760RSKR763->760ASA) Mutation：WT VWF Furin cleavage site mutated (760RSKR763->760ASA) Mutation：WT VWF Furin cleavage site mutated (760RSKR763->760ASA) Mutation：WT VWF Furin cleavage site mutated (760RSKR763->760ASA) Mutation：WT VWF Furin cleavage site mutated (760RSKR763->760ASA) Mutation：WT VWF Furin cleavage site mutated (760RSKR763->760ASA) Mutation：WT VWF Furin cleavage site mutated (760RSKR763->760ASA) Mutation：WT VWF Furin cleavage site mutated (760RSKR763->760ASA) Mutation：WT VWF Furin cleavage site mutated (760RSKR763->760ASA) Mutation：WT VWF Furin cleavage site mutated (760RSKR763->760ASA) Mutation：WT VWF Furin cleavage site mutated (760RSKR763->760ASA) Mutation：WT VWF Furin cleavage site mutated (760RSKR763->760ASA) Mutation：WT VWF Furin cleavage site mutated (760RSKR763->760ASA) Mutation：WT VWF Furin cleavage site mutated (760RSKR763->760ASA) CA CALCIUM ION × 48 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 80 ELECTRON MICROSCOPY
cryo-EM buffer pH 5.2；100 mM Sodium Cacodylate at pH 5.2, 10 mM CaCl2, and 100 mM NaCl.
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
8D3D VWF tubule derived from dimeric D1-A1 Deposited 2022-06-01 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 16 PDB declaration: hexadecameric
Chain A 1–1464（1464 aa）
Chain B 1–1464（1464 aa）
Chain C 1–1464（1464 aa）
Chain D 1–1464（1464 aa）
Chain E 1–1464（1464 aa）
Chain F 1–1464（1464 aa）
Chain G 1–1464（1464 aa）
Chain H 1–1464（1464 aa）
Chain I 1–1464（1464 aa）
Chain J 1–1464（1464 aa）
Chain K 1–1464（1464 aa）
Chain L 1–1464（1464 aa）
Chain M 1–1464（1464 aa）
Chain N 1–1464（1464 aa）
Chain O 1–1464（1464 aa）
Chain P 1–1464（1464 aa）
Mutation：Furin cleavage site mutated (760RSKR->760ASA) Mutation：Furin cleavage site mutated (760RSKR->760ASA) Mutation：Furin cleavage site mutated (760RSKR->760ASA) Mutation：Furin cleavage site mutated (760RSKR->760ASA) Mutation：Furin cleavage site mutated (760RSKR->760ASA) Mutation：Furin cleavage site mutated (760RSKR->760ASA) Mutation：Furin cleavage site mutated (760RSKR->760ASA) Mutation：Furin cleavage site mutated (760RSKR->760ASA) Mutation：Furin cleavage site mutated (760RSKR->760ASA) Mutation：Furin cleavage site mutated (760RSKR->760ASA) Mutation：Furin cleavage site mutated (760RSKR->760ASA) Mutation：Furin cleavage site mutated (760RSKR->760ASA) Mutation：Furin cleavage site mutated (760RSKR->760ASA) Mutation：Furin cleavage site mutated (760RSKR->760ASA) Mutation：Furin cleavage site mutated (760RSKR->760ASA) Mutation：Furin cleavage site mutated (760RSKR->760ASA) CA CALCIUM ION × 48 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 80 ELECTRON MICROSCOPY
cryo-EM buffer pH 5.2；100 mM Sodium Cacodylate at pH 5.2, 10 mM CaCl2, and 100 mM NaCl
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å

46 other PDB entries and 64 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name VWF_HUMAN
Isoform —
PDB entities 1, 2
Chains and sequence ranges Author chain A； PDBConstruct 1–741； UniProt 23–763 Author chain B； PDBConstruct 1–741； UniProt 23–763 Author chain E； PDBConstruct 1–741； UniProt 23–763 Author chain F； PDBConstruct 1–741； UniProt 23–763 Author chain G； PDBConstruct 1–741； UniProt 23–763 Author chain H； PDBConstruct 1–741； UniProt 23–763 Author chain I； PDBConstruct 1–741； UniProt 23–763 Author chain J； PDBConstruct 1–741； UniProt 23–763 Author chain K； PDBConstruct 1–741； UniProt 23–763 Author chain L； PDBConstruct 1–741； UniProt 23–763 Author chain M； PDBConstruct 1–741； UniProt 23–763 Author chain N； PDBConstruct 1–741； UniProt 23–763 Author chain O； PDBConstruct 1–741； UniProt 23–763 Author chain P； PDBConstruct 1–741； UniProt 23–763 Author chain Q； PDBConstruct 1–741； UniProt 23–763 Author chain R； PDBConstruct 1–741； UniProt 23–763 Author chain C； PDBConstruct 1–478； UniProt 764–1241 Author chain D； PDBConstruct 1–478； UniProt 764–1241 Author chain S； PDBConstruct 1–478； UniProt 764–1241 Author chain T； PDBConstruct 1–478； UniProt 764–1241 Author chain U； PDBConstruct 1–478； UniProt 764–1241 Author chain V； PDBConstruct 1–478； UniProt 764–1241 Author chain W； PDBConstruct 1–478； UniProt 764–1241 Author chain X； PDBConstruct 1–478； UniProt 764–1241 Author chain Y； PDBConstruct 1–478； UniProt 764–1241 Author chain Z； PDBConstruct 1–478； UniProt 764–1241 Author chain a； PDBConstruct 1–478； UniProt 764–1241 Author chain b； PDBConstruct 1–478； UniProt 764–1241 Author chain c； PDBConstruct 1–478； UniProt 764–1241 Author chain d； PDBConstruct 1–478； UniProt 764–1241 Author chain e； PDBConstruct 1–478； UniProt 764–1241 Author chain f； PDBConstruct 1–478； UniProt 764–1241

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

SAXS scattering curve SAXS Profile

SAXS profile for 7wpr

P(r) Distance Distribution P(r) Distribution

P(r) distribution for 7wpr
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2. Structure Basics 2. Structure Basics

Entry ID entry_id7wpr
Deposition date deposition_date2022-01-24
Structure title titleVWF D'D3 dimer complexed with D1D2 at 4.39 angstron resolution(VWF tube)
Keywords keywords;blood, VWF, von Willebrand factor, von Willebrand disease, blood coagulation, blood clotting, multimer assembly, VWF assembly, D'D3 domain, D1D2 domain, D'D3 dimer, D1D2 Dimer, VWF Tube, repeating unit ;; BLOOD CLOTTING
Experimental Method methodELECTRON MICROSCOPY
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3. SAXS Parameters (CRYSOL theoretical calculation) 3. SAXS Parameters (CRYSOL)

Radius of gyration Rg (Guinier) rg_guinier— Å
Radius of gyration Rg (electron density) rg_electron118.50 Å
Forward intensity I(0) i069239200000.00
Molecular weight molecular_weight2098000.0 kDa
Excluded volume excluded_volume2558100 ų
Envelope volume envelope_volume5821200 ų
Hydration-shell volume shell_volume409330 ų
Envelope diameter envelope_diameter396.3 Å
Shell Rg shell_rg125.40 Å
Envelope Rg envelope_rg107.50 Å
Shape Rg shape_rg118.50 Å
Total Rg total_rg118.60 Å
Total atoms total_atoms145668
Residues n_residues19024
Spherical-harmonic order n_harmonics20
q range q_range— – 0.5000 Å−1
Data points n_points101
Shell type shell_typedirectional
Solvent electron density solvent_density0.3340 e/ų
Shell contrast contrast_shell0.0300 e/ų
CRYSOL version crysol_version4.1.3
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4. P(r) Distance Distribution (GNOM inversion) 4. P(r) Analysis (GNOM)

Maximum dimension Dmax dmax302.8 Å
Rg (real space) rg_real116.00 Å
Rg uncertainty (real space) rg_real_error0.58 Å
I(0) (real space) i0_real6.6380e+10
I(0) uncertainty (real space) i0_real_error1.3420e+09
Rg (reciprocal space) rg_reciprocal126.20 Å
I(0) (reciprocal space) i0_reciprocal71050000000.0000
Solution quality estimate total_estimate0.8867
Solution quality rating solution_quality GOOD a GOOD solution
P(r) peaks n_peaks1
Primary peak position r_peak_primary163.1 Å
Skewness Skewness skewness-0.166
Kurtosis Kurtosis kurtosis-0.442
Angular range angular_range— – 0.0650 Å−1
Current regularization parameter α current_alpha1.3290
Highest regularization parameter α highest_alpha2078000000.0000
Real-space data points n_real_points14
GNOM version gnom_version4.1.3
Quality Criteria quality_criteria AN1: 0.000; Oscil: 0.905; Stabil: 0.959; Sysdev: 1.000; Positv: 1.000; Valcen: 0.939; Smooth: 0.000
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5. Crystallography and Experiment 5. Crystallography & Experiment

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6. Entities and Polymers Entities & Polymers (4)

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8. Citations (1)

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9. Files and Curves (10)