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4c2b

Crystal Structure of High-Affinity von Willebrand Factor A1 domain with Disulfide Mutation in Complex with High Affinity GPIb alpha

Method: X-RAY DIFFRACTION Dmax: 138.9 Å Quality: GOOD
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1. Protein Identity and Related Structures Protein Identity & Related Structures

VON WILLEBRAND FACTOR

HOMO SAPIENS

UniProt P04275

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 2 PDB declaration: dimeric（2） Consistent with protein copy count Chain A； UniProt 1264–1471 Fragment：1264-1468 Mutation：YES PLATELET GLYCOPROTEIN IB ALPHA CHAIN × 1 （P07359） SO4 SULFATE ION × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 1 X-RAY DIFFRACTION X-ray crystallization conditions：pH 4.6；CRYSTALS OF A1/SS-GPIB/VWD2 COMPLEX APPEARED IN DROPS WITH 20% PEG 4000, 0.16 M AMMONIUM SULFATE, 0.08M SODIUM ACETATE, PH 4.6, AND 20% GLYCEROL. THESE CRYSTALS WERE CRUSHED AND USED FOR SEEDING CRYSTAL GROWTH IN 8 MG/ML COMPLEX, 15% PEG 4000, 0.16 M AMMONIUM SULFATE, 0.08 M SODIUM ACETATE, PH 4.6, AND 20% GLYCEROL. SINCE THESE CRYSTALS WERE FORMED IN BUFFER CONTAINING 20% GLYCEROL, NO ADDITIONAL CRYOPROTECTION WAS USED. Resolution 2.80 Å R-free 0.286
2 Protein heterocomplex Heteromer Protein × 2 PDB declaration: dimeric（2） Consistent with protein copy count Chain C； UniProt 1264–1471 Fragment：1264-1468 Mutation：YES PLATELET GLYCOPROTEIN IB ALPHA CHAIN × 1 （P07359） SO4 SULFATE ION × 2 X-RAY DIFFRACTION X-ray crystallization conditions：pH 4.6；CRYSTALS OF A1/SS-GPIB/VWD2 COMPLEX APPEARED IN DROPS WITH 20% PEG 4000, 0.16 M AMMONIUM SULFATE, 0.08M SODIUM ACETATE, PH 4.6, AND 20% GLYCEROL. THESE CRYSTALS WERE CRUSHED AND USED FOR SEEDING CRYSTAL GROWTH IN 8 MG/ML COMPLEX, 15% PEG 4000, 0.16 M AMMONIUM SULFATE, 0.08 M SODIUM ACETATE, PH 4.6, AND 20% GLYCEROL. SINCE THESE CRYSTALS WERE FORMED IN BUFFER CONTAINING 20% GLYCEROL, NO ADDITIONAL CRYOPROTECTION WAS USED. Resolution 2.80 Å R-free 0.286
3 Protein heterocomplex Heteromer Protein × 2 PDB declaration: dimeric（2） Consistent with protein copy count Chain E； UniProt 1264–1471 Fragment：1264-1468 Mutation：YES PLATELET GLYCOPROTEIN IB ALPHA CHAIN × 1 （P07359） SO4 SULFATE ION × 2 X-RAY DIFFRACTION X-ray crystallization conditions：pH 4.6；CRYSTALS OF A1/SS-GPIB/VWD2 COMPLEX APPEARED IN DROPS WITH 20% PEG 4000, 0.16 M AMMONIUM SULFATE, 0.08M SODIUM ACETATE, PH 4.6, AND 20% GLYCEROL. THESE CRYSTALS WERE CRUSHED AND USED FOR SEEDING CRYSTAL GROWTH IN 8 MG/ML COMPLEX, 15% PEG 4000, 0.16 M AMMONIUM SULFATE, 0.08 M SODIUM ACETATE, PH 4.6, AND 20% GLYCEROL. SINCE THESE CRYSTALS WERE FORMED IN BUFFER CONTAINING 20% GLYCEROL, NO ADDITIONAL CRYOPROTECTION WAS USED. Resolution 2.80 Å R-free 0.286
4 Protein heterocomplex Heteromer Protein × 2 PDB declaration: dimeric（2） Consistent with protein copy count Chain G； UniProt 1264–1471 Fragment：1264-1468 Mutation：YES PLATELET GLYCOPROTEIN IB ALPHA CHAIN × 1 （P07359） SO4 SULFATE ION × 4 PEG DI(HYDROXYETHYL)ETHER × 1 X-RAY DIFFRACTION X-ray crystallization conditions：pH 4.6；CRYSTALS OF A1/SS-GPIB/VWD2 COMPLEX APPEARED IN DROPS WITH 20% PEG 4000, 0.16 M AMMONIUM SULFATE, 0.08M SODIUM ACETATE, PH 4.6, AND 20% GLYCEROL. THESE CRYSTALS WERE CRUSHED AND USED FOR SEEDING CRYSTAL GROWTH IN 8 MG/ML COMPLEX, 15% PEG 4000, 0.16 M AMMONIUM SULFATE, 0.08 M SODIUM ACETATE, PH 4.6, AND 20% GLYCEROL. SINCE THESE CRYSTALS WERE FORMED IN BUFFER CONTAINING 20% GLYCEROL, NO ADDITIONAL CRYOPROTECTION WAS USED. Resolution 2.80 Å R-free 0.286

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 4C2B Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
1AO3 A3 DOMAIN OF VON WILLEBRAND FACTOR Deposited 1997-07-16 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 1686–1872（187 aa） Fragment：A3 DOMAIN
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions pH 8.5；0.1M TRIS PH 8.5, 200MM MGCL2, 24% PEG 8K
Resolution 2.20 Å R-free 0.215
1AO3 A3 DOMAIN OF VON WILLEBRAND FACTOR Deposited 1997-07-16 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain B 1686–1872（187 aa） Fragment：A3 DOMAIN
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions pH 8.5；0.1M TRIS PH 8.5, 200MM MGCL2, 24% PEG 8K
Resolution 2.20 Å R-free 0.215
1ATZ HUMAN VON WILLEBRAND FACTOR A3 DOMAIN Deposited 1997-08-15 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 1685–1873（189 aa） Fragment：A3-DOMAIN
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions pH 8.5；24% PEG 3000, 200 MM SODIUM ACETATE, 100 MM TRIS/HCL PH 8.5
Resolution 1.80 Å R-free 0.240
1ATZ HUMAN VON WILLEBRAND FACTOR A3 DOMAIN Deposited 1997-08-15 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain B 1685–1873（189 aa） Fragment：A3-DOMAIN
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions pH 8.5；24% PEG 3000, 200 MM SODIUM ACETATE, 100 MM TRIS/HCL PH 8.5
Resolution 1.80 Å R-free 0.240
1AUQ A1 DOMAIN OF VON WILLEBRAND FACTOR Deposited 1997-09-01 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 1261–1468（208 aa） Fragment：A1
Not recorded CD CADMIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions pH 8.5；0.1M TRIS PH 8.5, 5MM CDCL2, 5% PEG 8K
Resolution 2.30 Å R-free 0.248
1FE8 CRYSTAL STRUCTURE OF THE VON WILLEBRAND FACTOR A3 DOMAIN IN COMPLEX WITH A FAB FRAGMENT OF IGG RU5 THAT INHIBITS COLLAGEN BINDING Deposited 2000-07-21 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 3 PDB declaration: trimeric
Chain A 1683–1874（192 aa） Fragment：COLLAGEN BINDING DOMAIN A3
Non-standard monomer：Yes (specific site not provided by mmCIF) NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 1 CAC CACODYLATE ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 5.5；277 K；iso-propanol, MPD, cacodylate, sodium chloride, Tris , pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 2.03 Å R-free 0.264
1FE8 CRYSTAL STRUCTURE OF THE VON WILLEBRAND FACTOR A3 DOMAIN IN COMPLEX WITH A FAB FRAGMENT OF IGG RU5 THAT INHIBITS COLLAGEN BINDING Deposited 2000-07-21 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Other combination Heteromer；Protein × 3 PDB declaration: trimeric
Chain B 1683–1874（192 aa） Fragment：COLLAGEN BINDING DOMAIN A3
Non-standard monomer：Yes (specific site not provided by mmCIF) NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 5.5；277 K；iso-propanol, MPD, cacodylate, sodium chloride, Tris , pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 2.03 Å R-free 0.264
1FE8 CRYSTAL STRUCTURE OF THE VON WILLEBRAND FACTOR A3 DOMAIN IN COMPLEX WITH A FAB FRAGMENT OF IGG RU5 THAT INHIBITS COLLAGEN BINDING Deposited 2000-07-21 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 3 Other combination Heteromer；Protein × 3 PDB declaration: trimeric
Chain C 1683–1874（192 aa） Fragment：COLLAGEN BINDING DOMAIN A3
Non-standard monomer：Yes (specific site not provided by mmCIF) No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 5.5；277 K；iso-propanol, MPD, cacodylate, sodium chloride, Tris , pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 2.03 Å R-free 0.264
1FE8 CRYSTAL STRUCTURE OF THE VON WILLEBRAND FACTOR A3 DOMAIN IN COMPLEX WITH A FAB FRAGMENT OF IGG RU5 THAT INHIBITS COLLAGEN BINDING Deposited 2000-07-21 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 4 Other combination Heteromer；Protein × 18 PDB declaration: octadecameric
Chain A 1683–1874（192 aa） Fragment：COLLAGEN BINDING DOMAIN A3
Chain B 1683–1874（192 aa） Fragment：COLLAGEN BINDING DOMAIN A3
Chain C 1683–1874（192 aa） Fragment：COLLAGEN BINDING DOMAIN A3
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 4 CAC CACODYLATE ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 5.5；277 K；iso-propanol, MPD, cacodylate, sodium chloride, Tris , pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 2.03 Å R-free 0.264
1FNS CRYSTAL STRUCTURE OF THE VON WILLEBRAND FACTOR (VWF) A1 DOMAIN I546V MUTANT IN COMPLEX WITH THE FUNCTION BLOCKING FAB NMC4 Deposited 2000-08-23 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain A 1271–1465（195 aa） Fragment：A1 DOMAIN RESIDUES 507 - 702, OR GLYCOPROTEIN IBA (A\:ALPHA) BINDING DOMAIN
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION mmCIF provides none of the parsed conditions Resolution 2.00 Å R-free 0.207
1IJB The von Willebrand Factor mutant (I546V) A1 domain Deposited 2001-04-25 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 1263–1464（202 aa） Fragment：A1 domain
Mutation：I546V No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 5.6；293 K；PEG 4000, sodium citrate, ammonium acetate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K
Resolution 1.80 Å R-free 0.208
1IJK The von Willebrand Factor mutant (I546V) A1 domain-botrocetin Complex Deposited 2001-04-26 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain A 1263–1464（202 aa） Fragment：A1 domain
Mutation：I546V No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 5.6；293 K；PEG 4000, sodium citrate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K
Resolution 2.60 Å R-free 0.279
1M10 Crystal structure of the complex of Glycoprotein Ib alpha and the von Willebrand Factor A1 Domain Deposited 2002-06-16 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1261–1468（208 aa） Fragment：A1 domain
Mutation：R543Q No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 5.5；277 K；PEG 3000, sodium chloride, MES, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 3.10 Å R-free 0.296
1OAK CRYSTAL STRUCTURE OF THE VON WILLEBRAND FACTOR (VWF) A1 DOMAIN IN COMPLEX WITH THE FUNCTION BLOCKING NMC-4 FAB Deposited 1997-12-18 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain A 1271–1465（195 aa） Fragment：A1 DOMAIN RESIDUES 507 - 702, OR GLYCOPROTEIN IBA (A\:ALPHA) BINDING DOMAIN
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions pH 8；pH 8
Resolution 2.20 Å R-free 0.274
1SQ0 Crystal Structure of the Complex of the Wild-type Von Willebrand Factor A1 domain and Glycoprotein Ib alpha at 2.6 Angstrom Resolution Deposited 2004-03-17 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1259–1472（214 aa） Fragment：A1
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8；291 K；PEG-3350; potassium thiocyanate, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K
Resolution 2.60 Å R-free 0.239
1U0N The ternary von Willebrand Factor A1-glycoprotein Ibalpha-botrocetin complex Deposited 2004-07-13 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric
Chain A 1261–1468（208 aa） Fragment：VWFA 1
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.5；277 K；Sodium citrate, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K
Resolution 2.95 Å R-free 0.276
1UEX Crystal structure of von Willebrand Factor A1 domain complexed with snake venom bitiscetin Deposited 2003-05-22 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain C 1260–1468（209 aa） Fragment：A1 domain
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.8；283 K；10% PEG 6000, 2% PEG-MME 550, 5% MPD, pH 7.8, VAPOR DIFFUSION, SITTING DROP, temperature 283K
Resolution 2.85 Å R-free 0.276
2ADF Crystal Structure and Paratope Determination of 82D6A3, an Antithrombotic Antibody Directed Against the von Willebrand factor A3-Domain Deposited 2005-07-20 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain A 1683–1874（192 aa） Fragment：A3 domain
Not recorded ACY ACETIC ACID × 1 SO4 SULFATE ION × 2 GOL GLYCEROL × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 4.6；293 K；PEG 8000, ammonium sulphate, acetate, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K
Resolution 1.90 Å R-free 0.220
2MHP Solution structure of the major factor VIII binding region on von Willebrand factor Deposited 2013-12-02 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 766–864（99 aa） Fragment：;domains TIL' and E', UNP residues 766-864 ;
Not recorded No recorded non-water small molecule SOLUTION NMR
NMR measurement conditions pH 7.4；298 K；Pressure ambient
NMR sample composition 300 mM [U-13C; U-15N] VWF TIL'E'-1, 20 mM sodium phosphate-2, 100 mM sodium chloride-3, 90% H2O/10% D2O | 90% H2O/10% D2O
NMR sample composition 300 mM [U-10% 13C] VWF TIL'E'-4, 20 mM sodium phosphate-5, 100 mM sodium chloride-6, 90% H2O/10% D2O | 90% H2O/10% D2O
NMR sample composition 300 mM [U-15N] VWF TIL'E'-7, 20 mM sodium phosphate-8, 100 mM sodium chloride-9, 90% H2O/10% D2O | 90% H2O/10% D2O
NMR sample composition 300 mM [U-13C; U-15N] VWF TIL'E'-10, 20 mM sodium phosphate-11, 100 mM sodium chloride-12, 90% H2O/10% D2O | 90% H2O/10% D2O
NMR sample composition 300 mM [U-15N] VWF TIL'E'-13, 20 mM sodium phosphate-14, 100 mM sodium chloride-15, 90% H2O/10% D2O | 90% H2O/10% D2O
NMR sample composition 300 mM [U-13C; U-15N] VWF TIL'E'-16, 20 mM sodium phosphate-17, 100 mM sodium chloride-18, 90% H2O/10% D2O | 90% H2O/10% D2O
NMR sample composition 300 mM [U-13C; U-15N] VWF TIL'E'-19, 20 mM sodium phosphate-20, 100 mM sodium chloride-21, 5 mg/mL Pf1 phage-22, 90% H2O/10% D2O | 90% H2O/10% D2O
NMR sample composition 300 mM [U-13C; U-15N] VWF TIL'E'-23, 20 mM sodium phosphate-24, 100 mM sodium chloride-25, 2 % w/v pentaethylene glycol dodecyl ether-26, 50 mM n-hexanol-27, 90% H2O/10% D2O | 90% H2O/10% D2O
Resolution not provided
2MHQ Solution structure of the major factor VIII binding region on von Willebrand factor Deposited 2013-12-02 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 766–864（99 aa） Fragment：;Domains TIL' and E', UNP residues 766-864 ;
Not recorded No recorded non-water small molecule SOLUTION NMR
NMR measurement conditions pH 7.4；298 K；Pressure ambient
NMR sample composition 300 mM [U-13C; U-15N] VWF TIL'E'-1, 20 mM sodium phosphate-2, 100 mM sodium chloride-3, 90% H2O/10% D2O | 90% H2O/10% D2O
NMR sample composition 300 mM [U-10% 13C] VWF TIL'E'-4, 20 mM sodium phosphate-5, 100 mM sodium chloride-6, 90% H2O/10% D2O | 90% H2O/10% D2O
NMR sample composition 300 mM [U-15N] VWF TIL'E'-7, 20 mM sodium phosphate-8, 100 mM sodium chloride-9, 90% H2O/10% D2O | 90% H2O/10% D2O
NMR sample composition 300 mM [U-13C; U-15N] VWF TIL'E'-10, 20 mM sodium phosphate-11, 100 mM sodium chloride-12, 90% H2O/10% D2O | 90% H2O/10% D2O
NMR sample composition 300 mM [U-15N] VWF TIL'E'-13, 20 mM sodium phosphate-14, 100 mM sodium chloride-15, 90% H2O/10% D2O | 90% H2O/10% D2O
NMR sample composition 300 mM [U-13C; U-15N] VWF TIL'E'-16, 20 mM sodium phosphate-17, 100 mM sodium chloride-18, 90% H2O/10% D2O | 90% H2O/10% D2O
NMR sample composition 300 mM [U-13C; U-15N] VWF TIL'E'-19, 20 mM sodium phosphate-20, 100 mM sodium chloride-21, 5 mg/mL Pf1 phage-22, 90% H2O/10% D2O | 90% H2O/10% D2O
NMR sample composition 300 mM [U-13C; U-15N] VWF TIL'E'-23, 20 mM sodium phosphate-24, 100 mM sodium chloride-25, 2 % w/v pentaethylene glycol dodecyl ether-26, 50 mM n-hexanol-27, 90% H2O/10% D2O | 90% H2O/10% D2O
Resolution not provided
3GXB Crystal structure of VWF A2 domain Deposited 2009-04-02 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Monomer；Protein × 1 PDB declaration: monomeric
Chain A 1495–1671（177 aa） Fragment：ADAMTS13 cleavage domain, residues 1495-1671
Not recorded NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 1 X-RAY DIFFRACTION
X-ray crystallization conditions pH 7.2；277 K；0.1 M Bis-Tris pH 7.2, 0.2 M lithium sulfate, 25 % PEG3350, vapor diffusion, hanging drop, temperature 277K
Resolution 1.90 Å R-free 0.224
3GXB Crystal structure of VWF A2 domain Deposited 2009-04-02 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Other combination Monomer；Protein × 1 PDB declaration: monomeric
Chain B 1495–1671（177 aa） Fragment：ADAMTS13 cleavage domain, residues 1495-1671
Not recorded NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 1 SO4 SULFATE ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions pH 7.2；277 K；0.1 M Bis-Tris pH 7.2, 0.2 M lithium sulfate, 25 % PEG3350, vapor diffusion, hanging drop, temperature 277K
Resolution 1.90 Å R-free 0.224
3HXO Crystal Structure of Von Willebrand Factor (VWF) A1 Domain in Complex with DNA Aptamer ARC1172, an Inhibitor of VWF-Platelet Binding Deposited 2009-06-21 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–DNA Monomer；Protein × 1 PDB declaration: dimeric
Chain A 1260–1468（209 aa） Fragment：von Willebrand factor (VWF) A1 Domain (residues 1260-1468)
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions pH 7.1；293 K；25% PEG3350, 0.16 M sodium fluoride, pH 7.1, EVAPORATION, temperature 293K
Resolution 2.40 Å R-free 0.274
3HXQ Crystal Structure of Von Willebrand Factor (VWF) A1 Domain in Complex with DNA Aptamer ARC1172, an Inhibitor of VWF-Platelet Binding Deposited 2009-06-21 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–DNA Monomer；Protein × 1 PDB declaration: dimeric
Chain A 1260–1468（209 aa） Fragment：von Willebrand factor (VWF) A1 Domain (residues 1260-1468)
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions pH 4.6；293 K；0.2 M NH4Ac, 0.1 M NaAc, 30% PEG4000, pH 4.6, EVAPORATION, temperature 293K
Resolution 2.69 Å R-free 0.279
3PPV Crystal structure of an engineered VWF A2 domain (N1493C and C1670S) Deposited 2010-11-25 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 1488–1674（187 aa） Fragment：VWFA 2 domain, UNP residues 1488-1674
Mutation：N1493C, C1670S SO4 SULFATE ION × 1 CA CALCIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 5.2；293 K；0.1M sodium citrate, 1.6M (NH4)2SO4, pH 5.2, VAPOR DIFFUSION, HANGING DROP, temperature 293K
Resolution 1.90 Å R-free 0.231
3PPW Crystal structure of the D1596A mutant of an engineered VWF A2 domain (N1493C and C1670S) Deposited 2010-11-25 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 1488–1674（187 aa） Fragment：VWFA 2 domain, UNP residues 1488-1674
Mutation：N1493C, D1596A, C1670S NA SODIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 5.2；293 K；0.1M sodium citrate, 1.6M (NH4)2SO4, pH 5.2, VAPOR DIFFUSION, HANGING DROP, temperature 293K
Resolution 1.90 Å R-free 0.226
3PPX Crystal structure of the N1602A mutant of an engineered VWF A2 domain (N1493C and C1670S) Deposited 2010-11-25 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 1488–1674（187 aa） Fragment：VWF A2 domain, UNP residues 1488-1674
Mutation：N1493C, N1602A, C1670S NA SODIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 5.2；293 K；0.1M sodium citrate, 1.6M (NH4)2SO4, pH 5.2, VAPOR DIFFUSION, HANGING DROP, temperature 293K
Resolution 1.91 Å R-free 0.237
3PPY Crystal structure of the D1596A/N1602A double mutant of an engineered VWF A2 domain (N1493C and C1670S) Deposited 2010-11-25 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 1488–1674（187 aa） Fragment：VWF A2 domain, UNP residues 1488-1674
Mutation：N1493, D1596A, N1602A, C1670S NA SODIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 5.2；293 K；0.1M sodium citrate, 1.6M (NH4)2SO4, pH 5.2, VAPOR DIFFUSION, HANGING DROP, temperature 293K
Resolution 2.00 Å R-free 0.232
3ZQK Von Willebrand Factor A2 domain with calcium Deposited 2011-06-09 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 1478–1674（197 aa） Fragment：A2 DOMAIN, RESIDUES 1478-1674
Not recorded GOL GLYCEROL × 2 CA CALCIUM ION × 1 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 2 X-RAY DIFFRACTION
X-ray crystallization conditions PROTEIN BUFFER: 0.015 M HEPES PH 7.5, 0.02 M NACL, 0.001 M CACL2. RESERVOIR BUFFER: 0.1 M MMT (PH 4.0-5.5), 0.15 M (NH4)2SO4, 18-23% (W/V) PEG2000 MME.
Resolution 1.70 Å R-free 0.200
3ZQK Von Willebrand Factor A2 domain with calcium Deposited 2011-06-09 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain B 1478–1674（197 aa） Fragment：A2 DOMAIN, RESIDUES 1478-1674
Not recorded CA CALCIUM ION × 1 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 2 X-RAY DIFFRACTION
X-ray crystallization conditions PROTEIN BUFFER: 0.015 M HEPES PH 7.5, 0.02 M NACL, 0.001 M CACL2. RESERVOIR BUFFER: 0.1 M MMT (PH 4.0-5.5), 0.15 M (NH4)2SO4, 18-23% (W/V) PEG2000 MME.
Resolution 1.70 Å R-free 0.200
3ZQK Von Willebrand Factor A2 domain with calcium Deposited 2011-06-09 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain C 1478–1674（197 aa） Fragment：A2 DOMAIN, RESIDUES 1478-1674
Not recorded CA CALCIUM ION × 1 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 2 X-RAY DIFFRACTION
X-ray crystallization conditions PROTEIN BUFFER: 0.015 M HEPES PH 7.5, 0.02 M NACL, 0.001 M CACL2. RESERVOIR BUFFER: 0.1 M MMT (PH 4.0-5.5), 0.15 M (NH4)2SO4, 18-23% (W/V) PEG2000 MME.
Resolution 1.70 Å R-free 0.200
4C29 Crystal Structure of High-Affinity von Willebrand Factor A1 domain with Disulfide Mutation Deposited 2013-08-16 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 1264–1471（208 aa） Fragment：RESIDUES 1264-1471
Mutation：YES ACT ACETATE ION × 1 PEG DI(HYDROXYETHYL)ETHER × 2 CA CALCIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions pH 4.6；CRYSTALS OF A1/SS APPEARED IN DROPS CONTAINING 25% PEG3350 AND 0.2 M CALCIUM ACETATE. THESE CRYSTALS WERE CRUSHED AND USED FOR SEEDING. CRYSTALS GROWN IN 8 MG/ML PROTEIN, 15% PEG3350, AND 0.2 M CALCIUM ACETATE WERE HARVESTED, AND SOAKED IN THE SAME BUFFER CONTAINING 20% GLYCEROL FOR CRYOPROTECTION., pH 4.6
Resolution 2.20 Å R-free 0.235
4C29 Crystal Structure of High-Affinity von Willebrand Factor A1 domain with Disulfide Mutation Deposited 2013-08-16 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain B 1264–1471（208 aa） Fragment：RESIDUES 1264-1471
Mutation：YES PEG DI(HYDROXYETHYL)ETHER × 2 CA CALCIUM ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions pH 4.6；CRYSTALS OF A1/SS APPEARED IN DROPS CONTAINING 25% PEG3350 AND 0.2 M CALCIUM ACETATE. THESE CRYSTALS WERE CRUSHED AND USED FOR SEEDING. CRYSTALS GROWN IN 8 MG/ML PROTEIN, 15% PEG3350, AND 0.2 M CALCIUM ACETATE WERE HARVESTED, AND SOAKED IN THE SAME BUFFER CONTAINING 20% GLYCEROL FOR CRYOPROTECTION., pH 4.6
Resolution 2.20 Å R-free 0.235
4C2A Crystal Structure of High-Affinity von Willebrand Factor A1 domain with R1306Q and I1309V Mutations in Complex with High Affinity GPIb alpha Deposited 2013-08-16 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1264–1471（208 aa） Fragment：RESIDUES 1264-1471
Mutation：YES CAC CACODYLATE ION × 1 ACT ACETATE ION × 1 CA CALCIUM ION × 1 PEG DI(HYDROXYETHYL)ETHER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions pH 6.5；CRYSTALS OF A1/VWD2-GPIBALPA/VWD2 COMPLEX APPEARED IN 12% PEG 8000, 0.1 M SODIUM CACODYLATE PH 6.5, 0.1 M CALCIUM ACETATE, AND 0.4 MG/ML RISTOCETIN (SIGMA-ALDRICH). THESE CRYSTALS WERE CRUSHED AND USED FOR SEEDING. CRYSTALS GREW IN 10 MG/ML COMPLEX, 14% PEG8000, 0.2 M CALCIUM ACETATE, 0.1 M SODIUM CACODYLATE, PH 6.5, AND 0.4 MG/ML RISTOCETIN. NEXT WE SOAKED THESE CRYSTALS IN 14% PEG8000, 0.2 M CALCIUM ACETATE, 0.1 SODIUM CACODYLATE, PH 6.5, AND 4 MG/ML RISTOCETIN.
Resolution 2.08 Å R-free 0.189
4DMU Crystal structure of the von Willebrand factor A3 domain in complex with a collagen III derived triple-helical peptide Deposited 2012-02-08 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain B 1683–1874（192 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.5；277 K；100 mM cacodylate, pH 6.5, 1.20 M (NH4)2SO4, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 2.80 Å R-free 0.253
4DMU Crystal structure of the von Willebrand factor A3 domain in complex with a collagen III derived triple-helical peptide Deposited 2012-02-08 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain D 1683–1874（192 aa）
Not recorded SO4 SULFATE ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.5；277 K；100 mM cacodylate, pH 6.5, 1.20 M (NH4)2SO4, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 2.80 Å R-free 0.253
4DMU Crystal structure of the von Willebrand factor A3 domain in complex with a collagen III derived triple-helical peptide Deposited 2012-02-08 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 3 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain F 1683–1874（192 aa）
Not recorded SO4 SULFATE ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.5；277 K；100 mM cacodylate, pH 6.5, 1.20 M (NH4)2SO4, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 2.80 Å R-free 0.253
4DMU Crystal structure of the von Willebrand factor A3 domain in complex with a collagen III derived triple-helical peptide Deposited 2012-02-08 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 4 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain H 1683–1874（192 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.5；277 K；100 mM cacodylate, pH 6.5, 1.20 M (NH4)2SO4, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 2.80 Å R-free 0.253
4DMU Crystal structure of the von Willebrand factor A3 domain in complex with a collagen III derived triple-helical peptide Deposited 2012-02-08 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 5 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain J 1683–1874（192 aa）
Not recorded SO4 SULFATE ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.5；277 K；100 mM cacodylate, pH 6.5, 1.20 M (NH4)2SO4, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 2.80 Å R-free 0.253
4DMU Crystal structure of the von Willebrand factor A3 domain in complex with a collagen III derived triple-helical peptide Deposited 2012-02-08 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 6 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain L 1683–1874（192 aa）
Not recorded SO4 SULFATE ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.5；277 K；100 mM cacodylate, pH 6.5, 1.20 M (NH4)2SO4, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 2.80 Å R-free 0.253
4NT5 Crystal structure of human von Willebrand factor CTCK domain Deposited 2013-12-01 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 2721–2813（93 aa） Fragment：C-terminal cystine knot domain, unp residues 2721-2813
Not recorded NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 2 ZN ZINC ION × 2 SO4 SULFATE ION × 10 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8.3；293 K；1.3 M Lithium Sulfate, 0.1 M Bicine, pH 8.3, VAPOR DIFFUSION, HANGING DROP, temperature 293K
Resolution 3.28 Å R-free 0.248
5BV8 G1324S mutation in von Willebrand Factor A1 domain Deposited 2015-06-04 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 1238–1471（234 aa） Fragment：A1 domain (UNP residues 1238-1471)
Mutation：G1324S EDO 1,2-ETHANEDIOL × 1 CL CHLORIDE ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 10.5；298 K；30% PEG 400, 100 mM CAPS/Sodium hydroxide
Resolution 1.59 Å R-free 0.181
6FWN Structure and dynamics of the platelet integrin-binding C4 domain of von Willebrand factor Deposited 2018-03-06 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 2497–2577（81 aa）
Not recorded No recorded non-water small molecule SOLUTION NMR
NMR measurement conditions pH 6.5；298 K；Ionic strength (raw mmCIF value) 0.04；Pressure 1
NMR sample composition 500 uM [U-13C; U-15N] von Willebrand Factor C4 domain, 20 mM sodium phosphate, 49.5 M H2O, 5.5 M D2O, 0.02 % w/v sodium azide, 90% H2O/10% D2O | 90% H2O/10% D2O
NMR sample composition 500 uM [U-13C; U-15N] von Willebrand Factor C4 domain, 20 mM sodium phosphate, 55 M D2O, 5 mM H2O, 0.02 % w/v sodium azide, 100% D2O | 100% D2O
Resolution not provided
6N29 Crystal structure of monomeric von Willebrand Factor D`D3 assembly Deposited 2018-11-12 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 764–1244（481 aa）
Mutation：Q852R, C1009A, C1142A NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 3 CA CALCIUM ION × 2 PEG DI(HYDROXYETHYL)ETHER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；297 K；5.5% (w/v) PEG8000, 10% (w/v) PEG1000, 0.2 M calcium acetate and 0.1 M Tris-acetate, pH 8.5
Resolution 2.50 Å R-free 0.241
6N29 Crystal structure of monomeric von Willebrand Factor D`D3 assembly Deposited 2018-11-12 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain B 764–1244（481 aa）
Mutation：Q852R, C1009A, C1142A NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 2 CA CALCIUM ION × 1 PEG DI(HYDROXYETHYL)ETHER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；297 K；5.5% (w/v) PEG8000, 10% (w/v) PEG1000, 0.2 M calcium acetate and 0.1 M Tris-acetate, pH 8.5
Resolution 2.50 Å R-free 0.241
7EOW High-resolution structure of vWF A1 domain in complex with caplacizumab, the first nanobody-based medicine Deposited 2021-04-23 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1261–1468（208 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8；293.15 K；20 mM Tris-HCl pH 8 150 mM NaCl 10% glycerol 40% glycerol ethoxylate
Resolution 1.60 Å R-free 0.198
7F49 von Willebrand factor (VWF) A1 domain with BT-100 aptamer RNA Deposited 2021-06-18 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–DNA Monomer；Protein × 1 PDB declaration: dimeric
Chain A 1260–1468（209 aa）
Not recorded GOL GLYCEROL × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；291 K；0.1M Sodium Acetate pH 4.6, 18% w/v PEG 4000
Resolution 2.09 Å R-free 0.239
7P4N NMR solution structure of the C6 domain of von Willebrand Factor Deposited 2021-07-12 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 2647–2720（74 aa）
Mutation：G2705R No recorded non-water small molecule SOLUTION NMR
NMR measurement conditions pH 6.5；298 K；Ionic strength (raw mmCIF value) 20；Pressure 1.013
NMR sample composition 300 uM [U-13C; U-15N] von Willebrand Factor C6 domain, 20 mM None sodium phosphate, 90% H2O/10% D2O | 90% H2O/10% D2O
Resolution not provided
7PMV VWF Tubules of D1D2D'D3 domains Deposited 2021-09-02 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 1–1241（1241 aa）
Chain B 1–1241（1241 aa）
Chain D 1–1241（1241 aa）
Chain E 1–1241（1241 aa）
Not recorded NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 16 CA CALCIUM ION × 12 ELECTRON MICROSCOPY
cryo-EM buffer pH 6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.70 Å
7PNF VWF Tubules of D1D2D'D3 domains Deposited 2021-09-06 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Homooligomer；Protein × 36 PDB declaration: 36-meric
Chain B 1–1241（1241 aa）
Chain D 1–1241（1241 aa）
Not recorded NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 144 CA CALCIUM ION × 108 ELECTRON MICROSCOPY
cryo-EM buffer pH 6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.35 Å
7WN3 Cryo-EM structure of VWF D'D3 dimer (2M mutant) complexed with D1D2 at 3.29 angstron resolution (2 units) Deposited 2022-01-17 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 8 PDB declaration: octameric
Chain A 23–763（741 aa） Fragment：D1D2 domain
Chain B 23–763（741 aa） Fragment：D1D2 domain
Chain C 764–1241（478 aa） Fragment：;D'D3 domain ;
Chain D 764–1241（478 aa） Fragment：;D'D3 domain ;
Chain E 23–763（741 aa） Fragment：D1D2 domain
Chain F 764–1241（478 aa） Fragment：;D'D3 domain ;
Chain G 23–763（741 aa） Fragment：D1D2 domain
Chain H 764–1241（478 aa） Fragment：;D'D3 domain ;
Mutation：R1136M,E1143M Mutation：R1136M,E1143M Mutation：R1136M,E1143M Mutation：R1136M,E1143M CA CALCIUM ION × 16 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 20 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.29 Å
7WN4 Cryo-EM structure of VWF D'D3 dimer (wild type) complexed with D1D2 at 3.4 angstron resolution (1 unit) Deposited 2022-01-17 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain E 23–763（741 aa） Fragment：D1D2 domain
Chain F 764–1241（478 aa） Fragment：;D'D3 domain ;
Chain G 23–763（741 aa） Fragment：D1D2 domain
Chain H 764–1241（478 aa） Fragment：;D'D3 domain ;
Not recorded CA CALCIUM ION × 6 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
7WN6 Cryo-EM structure of VWF D'D3 dimer (R1136M/E1143M mutant) complexed with D1D2 at 3.29 angstron resolution (2 units) Deposited 2022-01-17 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 8 PDB declaration: octameric
Chain A 23–763（741 aa） Fragment：D1D2 domain
Chain B 23–763（741 aa） Fragment：D1D2 domain
Chain C 764–1241（478 aa） Fragment：;D'D3 domain ;
Chain D 764–1241（478 aa） Fragment：;D'D3 domain ;
Chain E 23–763（741 aa） Fragment：D1D2 domain
Chain F 764–1241（478 aa） Fragment：;D'D3 domain ;
Chain G 23–763（741 aa） Fragment：D1D2 domain
Chain H 764–1241（478 aa） Fragment：;D'D3 domain ;
Mutation：R1136M/E1143M Mutation：R1136M/E1143M Mutation：R1136M/E1143M Mutation：R1136M/E1143M CA CALCIUM ION × 16 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 20 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.29 Å
7WPP Cryo-EM structure of VWF D'D3 dimer complexed with D1D2 at 2.85 angstron resolution (1 unit) Deposited 2022-01-24 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain E 23–763（741 aa） Fragment：D1D2 domain
Chain F 764–1241（478 aa） Fragment：;D'D3 domain ;
Chain G 23–763（741 aa） Fragment：D1D2 domain
Chain H 764–1241（478 aa） Fragment：;D'D3 domain ;
Not recorded CA CALCIUM ION × 6 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 10 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.85 Å
7WPQ Cryo-EM structure of VWF D'D3 dimer complexed with D1D2 at 3.27 angstron resolution (2 units) Deposited 2022-01-24 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 8 PDB declaration: octameric
Chain A 23–763（741 aa） Fragment：D1D2 domain
Chain B 23–763（741 aa） Fragment：D1D2 domain
Chain C 764–1241（478 aa） Fragment：;D'D3 domain ;
Chain D 764–1241（478 aa） Fragment：;D'D3 domain ;
Chain E 23–763（741 aa） Fragment：D1D2 domain
Chain F 764–1241（478 aa） Fragment：;D'D3 domain ;
Chain G 23–763（741 aa） Fragment：D1D2 domain
Chain H 764–1241（478 aa） Fragment：;D'D3 domain ;
Not recorded NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 20 CA CALCIUM ION × 16 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.27 Å
7WPR VWF D'D3 dimer complexed with D1D2 at 4.39 angstron resolution(VWF tube) Deposited 2022-01-24 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 32 PDB declaration: 32-meric
Chain A 23–763（741 aa） Fragment：D1D2 domain
Chain B 23–763（741 aa） Fragment：D1D2 domain
Chain C 764–1241（478 aa） Fragment：;D'D3 domain ;
Chain D 764–1241（478 aa） Fragment：;D'D3 domain ;
Chain E 23–763（741 aa） Fragment：D1D2 domain
Chain F 23–763（741 aa） Fragment：D1D2 domain
Chain G 23–763（741 aa） Fragment：D1D2 domain
Chain H 23–763（741 aa） Fragment：D1D2 domain
Chain I 23–763（741 aa） Fragment：D1D2 domain
Chain J 23–763（741 aa） Fragment：D1D2 domain
Chain K 23–763（741 aa） Fragment：D1D2 domain
Chain L 23–763（741 aa） Fragment：D1D2 domain
Chain M 23–763（741 aa） Fragment：D1D2 domain
Chain N 23–763（741 aa） Fragment：D1D2 domain
Chain O 23–763（741 aa） Fragment：D1D2 domain
Chain P 23–763（741 aa） Fragment：D1D2 domain
Chain Q 23–763（741 aa） Fragment：D1D2 domain
Chain R 23–763（741 aa） Fragment：D1D2 domain
Chain S 764–1241（478 aa） Fragment：;D'D3 domain ;
Chain T 764–1241（478 aa） Fragment：;D'D3 domain ;
Chain U 764–1241（478 aa） Fragment：;D'D3 domain ;
Chain V 764–1241（478 aa） Fragment：;D'D3 domain ;
Chain W 764–1241（478 aa） Fragment：;D'D3 domain ;
Chain X 764–1241（478 aa） Fragment：;D'D3 domain ;
Chain Y 764–1241（478 aa） Fragment：;D'D3 domain ;
Chain Z 764–1241（478 aa） Fragment：;D'D3 domain ;
Chain a 764–1241（478 aa） Fragment：;D'D3 domain ;
Chain b 764–1241（478 aa） Fragment：;D'D3 domain ;
Chain c 764–1241（478 aa） Fragment：;D'D3 domain ;
Chain d 764–1241（478 aa） Fragment：;D'D3 domain ;
Chain e 764–1241（478 aa） Fragment：;D'D3 domain ;
Chain f 764–1241（478 aa） Fragment：;D'D3 domain ;
Not recorded NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 42 CA CALCIUM ION × 56 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.39 Å
7WPS Cryo-EM structure of VWF D'D3 dimer complexed with D1D2 at 4.3 angstron resolution (7 units) Deposited 2022-01-24 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 28 PDB declaration: 28-meric
Chain A 23–763（741 aa） Fragment：D1D2 domain
Chain B 23–763（741 aa） Fragment：D1D2 domain
Chain C 764–1241（478 aa） Fragment：;D'D3 domain ;
Chain D 764–1241（478 aa） Fragment：;D'D3 domain ;
Chain E 23–763（741 aa） Fragment：D1D2 domain
Chain F 764–1241（478 aa） Fragment：;D'D3 domain ;
Chain G 23–763（741 aa） Fragment：D1D2 domain
Chain H 764–1241（478 aa） Fragment：;D'D3 domain ;
Chain I 23–763（741 aa） Fragment：D1D2 domain
Chain J 764–1241（478 aa） Fragment：;D'D3 domain ;
Chain K 23–763（741 aa） Fragment：D1D2 domain
Chain L 764–1241（478 aa） Fragment：;D'D3 domain ;
Chain M 23–763（741 aa） Fragment：D1D2 domain
Chain N 764–1241（478 aa） Fragment：;D'D3 domain ;
Chain O 23–763（741 aa） Fragment：D1D2 domain
Chain P 764–1241（478 aa） Fragment：;D'D3 domain ;
Chain Q 23–763（741 aa） Fragment：D1D2 domain
Chain R 764–1241（478 aa） Fragment：;D'D3 domain ;
Chain S 23–763（741 aa） Fragment：D1D2 domain
Chain T 764–1241（478 aa） Fragment：;D'D3 domain ;
Chain U 23–763（741 aa） Fragment：D1D2 domain
Chain V 764–1241（478 aa） Fragment：;D'D3 domain ;
Chain W 23–763（741 aa） Fragment：D1D2 domain
Chain X 764–1241（478 aa） Fragment：;D'D3 domain ;
Chain Y 23–763（741 aa） Fragment：D1D2 domain
Chain Z 764–1241（478 aa） Fragment：;D'D3 domain ;
Chain a 23–763（741 aa） Fragment：D1D2 domain
Chain b 764–1241（478 aa） Fragment：;D'D3 domain ;
Not recorded NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 42 CA CALCIUM ION × 56 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.32 Å
7WQT Cryo-EM structure of VWF D'D3 dimer complexed with D1D2 at 4.3 angstron resolution (VWF tube) Deposited 2022-01-26 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 32 PDB declaration: 32-meric
Chain A 23–763（741 aa） Fragment：D1D2 domain
Chain B 23–763（741 aa） Fragment：D1D2 domain
Chain C 764–1241（478 aa） Fragment：;D'D3 domain ;
Chain D 764–1241（478 aa） Fragment：;D'D3 domain ;
Chain E 23–763（741 aa） Fragment：D1D2 domain
Chain F 23–763（741 aa） Fragment：D1D2 domain
Chain G 23–763（741 aa） Fragment：D1D2 domain
Chain H 23–763（741 aa） Fragment：D1D2 domain
Chain I 23–763（741 aa） Fragment：D1D2 domain
Chain J 23–763（741 aa） Fragment：D1D2 domain
Chain K 23–763（741 aa） Fragment：D1D2 domain
Chain L 23–763（741 aa） Fragment：D1D2 domain
Chain M 23–763（741 aa） Fragment：D1D2 domain
Chain N 23–763（741 aa） Fragment：D1D2 domain
Chain O 23–763（741 aa） Fragment：D1D2 domain
Chain P 23–763（741 aa） Fragment：D1D2 domain
Chain Q 23–763（741 aa） Fragment：D1D2 domain
Chain R 23–763（741 aa） Fragment：D1D2 domain
Chain S 764–1241（478 aa） Fragment：;D'D3 domain ;
Chain T 764–1241（478 aa） Fragment：;D'D3 domain ;
Chain U 764–1241（478 aa） Fragment：;D'D3 domain ;
Chain V 764–1241（478 aa） Fragment：;D'D3 domain ;
Chain W 764–1241（478 aa） Fragment：;D'D3 domain ;
Chain X 764–1241（478 aa） Fragment：;D'D3 domain ;
Chain Y 764–1241（478 aa） Fragment：;D'D3 domain ;
Chain Z 764–1241（478 aa） Fragment：;D'D3 domain ;
Chain a 764–1241（478 aa） Fragment：;D'D3 domain ;
Chain b 764–1241（478 aa） Fragment：;D'D3 domain ;
Chain c 764–1241（478 aa） Fragment：;D'D3 domain ;
Chain d 764–1241（478 aa） Fragment：;D'D3 domain ;
Chain e 764–1241（478 aa） Fragment：;D'D3 domain ;
Chain f 764–1241（478 aa） Fragment：;D'D3 domain ;
Not recorded NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 42 CA CALCIUM ION × 56 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.30 Å
7ZWH VWF Tubules of D1D2 and D'D3A1 domains Deposited 2022-05-19 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain D 1–1197（1197 aa）
Chain E 1–1197（1197 aa）
Chain G 1265–1463（199 aa）
Chain H 1265–1463（199 aa）
Not recorded NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 8 CA CALCIUM ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 5.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
8D3C VWF tubule derived from monomeric D1-A1 Deposited 2022-06-01 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 16 PDB declaration: hexadecameric
Chain A 1–1464（1464 aa）
Chain B 1–1464（1464 aa）
Chain C 1–1464（1464 aa）
Chain D 1–1464（1464 aa）
Chain E 1–1464（1464 aa）
Chain F 1–1464（1464 aa）
Chain G 1–1464（1464 aa）
Chain H 1–1464（1464 aa）
Chain I 1–1464（1464 aa）
Chain J 1–1464（1464 aa）
Chain K 1–1464（1464 aa）
Chain L 1–1464（1464 aa）
Chain M 1–1464（1464 aa）
Chain N 1–1464（1464 aa）
Chain O 1–1464（1464 aa）
Chain P 1–1464（1464 aa）
Mutation：WT VWF Furin cleavage site mutated (760RSKR763->760ASA) Mutation：WT VWF Furin cleavage site mutated (760RSKR763->760ASA) Mutation：WT VWF Furin cleavage site mutated (760RSKR763->760ASA) Mutation：WT VWF Furin cleavage site mutated (760RSKR763->760ASA) Mutation：WT VWF Furin cleavage site mutated (760RSKR763->760ASA) Mutation：WT VWF Furin cleavage site mutated (760RSKR763->760ASA) Mutation：WT VWF Furin cleavage site mutated (760RSKR763->760ASA) Mutation：WT VWF Furin cleavage site mutated (760RSKR763->760ASA) Mutation：WT VWF Furin cleavage site mutated (760RSKR763->760ASA) Mutation：WT VWF Furin cleavage site mutated (760RSKR763->760ASA) Mutation：WT VWF Furin cleavage site mutated (760RSKR763->760ASA) Mutation：WT VWF Furin cleavage site mutated (760RSKR763->760ASA) Mutation：WT VWF Furin cleavage site mutated (760RSKR763->760ASA) Mutation：WT VWF Furin cleavage site mutated (760RSKR763->760ASA) Mutation：WT VWF Furin cleavage site mutated (760RSKR763->760ASA) Mutation：WT VWF Furin cleavage site mutated (760RSKR763->760ASA) CA CALCIUM ION × 48 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 80 ELECTRON MICROSCOPY
cryo-EM buffer pH 5.2；100 mM Sodium Cacodylate at pH 5.2, 10 mM CaCl2, and 100 mM NaCl.
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
8D3D VWF tubule derived from dimeric D1-A1 Deposited 2022-06-01 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 16 PDB declaration: hexadecameric
Chain A 1–1464（1464 aa）
Chain B 1–1464（1464 aa）
Chain C 1–1464（1464 aa）
Chain D 1–1464（1464 aa）
Chain E 1–1464（1464 aa）
Chain F 1–1464（1464 aa）
Chain G 1–1464（1464 aa）
Chain H 1–1464（1464 aa）
Chain I 1–1464（1464 aa）
Chain J 1–1464（1464 aa）
Chain K 1–1464（1464 aa）
Chain L 1–1464（1464 aa）
Chain M 1–1464（1464 aa）
Chain N 1–1464（1464 aa）
Chain O 1–1464（1464 aa）
Chain P 1–1464（1464 aa）
Mutation：Furin cleavage site mutated (760RSKR->760ASA) Mutation：Furin cleavage site mutated (760RSKR->760ASA) Mutation：Furin cleavage site mutated (760RSKR->760ASA) Mutation：Furin cleavage site mutated (760RSKR->760ASA) Mutation：Furin cleavage site mutated (760RSKR->760ASA) Mutation：Furin cleavage site mutated (760RSKR->760ASA) Mutation：Furin cleavage site mutated (760RSKR->760ASA) Mutation：Furin cleavage site mutated (760RSKR->760ASA) Mutation：Furin cleavage site mutated (760RSKR->760ASA) Mutation：Furin cleavage site mutated (760RSKR->760ASA) Mutation：Furin cleavage site mutated (760RSKR->760ASA) Mutation：Furin cleavage site mutated (760RSKR->760ASA) Mutation：Furin cleavage site mutated (760RSKR->760ASA) Mutation：Furin cleavage site mutated (760RSKR->760ASA) Mutation：Furin cleavage site mutated (760RSKR->760ASA) Mutation：Furin cleavage site mutated (760RSKR->760ASA) CA CALCIUM ION × 48 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 80 ELECTRON MICROSCOPY
cryo-EM buffer pH 5.2；100 mM Sodium Cacodylate at pH 5.2, 10 mM CaCl2, and 100 mM NaCl
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å

46 other PDB entries and 61 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name VWF_HUMAN
Isoform —
PDB entities 1
Chains and sequence ranges Author chain A； PDBConstruct 2–209； UniProt 1264–1471 Author chain C； PDBConstruct 2–209； UniProt 1264–1471 Author chain E； PDBConstruct 2–209； UniProt 1264–1471 Author chain G； PDBConstruct 2–209； UniProt 1264–1471

PLATELET GLYCOPROTEIN IB ALPHA CHAIN

HOMO SAPIENS

UniProt P07359

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 2 PDB declaration: dimeric（2） Consistent with protein copy count Chain B； UniProt 17–306 Mutation：YES VON WILLEBRAND FACTOR × 1 （P04275） SO4 SULFATE ION × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 1 X-RAY DIFFRACTION X-ray crystallization conditions：pH 4.6；CRYSTALS OF A1/SS-GPIB/VWD2 COMPLEX APPEARED IN DROPS WITH 20% PEG 4000, 0.16 M AMMONIUM SULFATE, 0.08M SODIUM ACETATE, PH 4.6, AND 20% GLYCEROL. THESE CRYSTALS WERE CRUSHED AND USED FOR SEEDING CRYSTAL GROWTH IN 8 MG/ML COMPLEX, 15% PEG 4000, 0.16 M AMMONIUM SULFATE, 0.08 M SODIUM ACETATE, PH 4.6, AND 20% GLYCEROL. SINCE THESE CRYSTALS WERE FORMED IN BUFFER CONTAINING 20% GLYCEROL, NO ADDITIONAL CRYOPROTECTION WAS USED. Resolution 2.80 Å R-free 0.286
2 Protein heterocomplex Heteromer Protein × 2 PDB declaration: dimeric（2） Consistent with protein copy count Chain D； UniProt 17–306 Mutation：YES VON WILLEBRAND FACTOR × 1 （P04275） SO4 SULFATE ION × 2 X-RAY DIFFRACTION X-ray crystallization conditions：pH 4.6；CRYSTALS OF A1/SS-GPIB/VWD2 COMPLEX APPEARED IN DROPS WITH 20% PEG 4000, 0.16 M AMMONIUM SULFATE, 0.08M SODIUM ACETATE, PH 4.6, AND 20% GLYCEROL. THESE CRYSTALS WERE CRUSHED AND USED FOR SEEDING CRYSTAL GROWTH IN 8 MG/ML COMPLEX, 15% PEG 4000, 0.16 M AMMONIUM SULFATE, 0.08 M SODIUM ACETATE, PH 4.6, AND 20% GLYCEROL. SINCE THESE CRYSTALS WERE FORMED IN BUFFER CONTAINING 20% GLYCEROL, NO ADDITIONAL CRYOPROTECTION WAS USED. Resolution 2.80 Å R-free 0.286
3 Protein heterocomplex Heteromer Protein × 2 PDB declaration: dimeric（2） Consistent with protein copy count Chain F； UniProt 17–306 Mutation：YES VON WILLEBRAND FACTOR × 1 （P04275） SO4 SULFATE ION × 2 X-RAY DIFFRACTION X-ray crystallization conditions：pH 4.6；CRYSTALS OF A1/SS-GPIB/VWD2 COMPLEX APPEARED IN DROPS WITH 20% PEG 4000, 0.16 M AMMONIUM SULFATE, 0.08M SODIUM ACETATE, PH 4.6, AND 20% GLYCEROL. THESE CRYSTALS WERE CRUSHED AND USED FOR SEEDING CRYSTAL GROWTH IN 8 MG/ML COMPLEX, 15% PEG 4000, 0.16 M AMMONIUM SULFATE, 0.08 M SODIUM ACETATE, PH 4.6, AND 20% GLYCEROL. SINCE THESE CRYSTALS WERE FORMED IN BUFFER CONTAINING 20% GLYCEROL, NO ADDITIONAL CRYOPROTECTION WAS USED. Resolution 2.80 Å R-free 0.286
4 Protein heterocomplex Heteromer Protein × 2 PDB declaration: dimeric（2） Consistent with protein copy count Chain H； UniProt 17–306 Mutation：YES VON WILLEBRAND FACTOR × 1 （P04275） SO4 SULFATE ION × 4 PEG DI(HYDROXYETHYL)ETHER × 1 X-RAY DIFFRACTION X-ray crystallization conditions：pH 4.6；CRYSTALS OF A1/SS-GPIB/VWD2 COMPLEX APPEARED IN DROPS WITH 20% PEG 4000, 0.16 M AMMONIUM SULFATE, 0.08M SODIUM ACETATE, PH 4.6, AND 20% GLYCEROL. THESE CRYSTALS WERE CRUSHED AND USED FOR SEEDING CRYSTAL GROWTH IN 8 MG/ML COMPLEX, 15% PEG 4000, 0.16 M AMMONIUM SULFATE, 0.08 M SODIUM ACETATE, PH 4.6, AND 20% GLYCEROL. SINCE THESE CRYSTALS WERE FORMED IN BUFFER CONTAINING 20% GLYCEROL, NO ADDITIONAL CRYOPROTECTION WAS USED. Resolution 2.80 Å R-free 0.286

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 4C2B Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
1GWB STRUCTURE OF GLYCOPROTEIN 1B Deposited 2002-03-14 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 16–296（281 aa） Fragment：N-TERMINAL DOMAIN, RESIDUES 16-296
Non-standard monomer：Yes (specific site not provided by mmCIF) PT PLATINUM (II) ION × 2 ACY ACETIC ACID × 1 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 2 X-RAY DIFFRACTION
X-ray crystallization conditions pH 6.5；pH 6.50
Resolution 2.80 Å R-free 0.274
1GWB STRUCTURE OF GLYCOPROTEIN 1B Deposited 2002-03-14 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain B 16–296（281 aa） Fragment：N-TERMINAL DOMAIN, RESIDUES 16-296
Non-standard monomer：Yes (specific site not provided by mmCIF) PT PLATINUM (II) ION × 2 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 1 SO4 SULFATE ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions pH 6.5；pH 6.50
Resolution 2.80 Å R-free 0.274
1M0Z Crystal Structure of the von Willebrand Factor Binding Domain of Glycoprotein Ib alpha Deposited 2002-06-16 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 17–306（290 aa） Fragment：von Willebrand Factor binding domain
Mutation：N21Q N159Q No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8.2；301 K；ammonium sulfate, lithium sulfate, CAPS, pH 8.2, VAPOR DIFFUSION, HANGING DROP, temperature 301K
Resolution 1.85 Å R-free 0.216
1M0Z Crystal Structure of the von Willebrand Factor Binding Domain of Glycoprotein Ib alpha Deposited 2002-06-16 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain B 17–306（290 aa） Fragment：von Willebrand Factor binding domain
Mutation：N21Q N159Q No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8.2；301 K；ammonium sulfate, lithium sulfate, CAPS, pH 8.2, VAPOR DIFFUSION, HANGING DROP, temperature 301K
Resolution 1.85 Å R-free 0.216
1M10 Crystal structure of the complex of Glycoprotein Ib alpha and the von Willebrand Factor A1 Domain Deposited 2002-06-16 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain B 17–306（290 aa） Fragment：von Willebrand Factor binding domain
Mutation：N21Q N159Q M239V No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 5.5；277 K；PEG 3000, sodium chloride, MES, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 3.10 Å R-free 0.296
1OOK Crystal Structure of the Complex of Platelet Receptor GPIb-alpha and Human alpha-Thrombin Deposited 2003-03-03 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 4 PDB declaration: tetrameric
Chain G 17–306（290 aa） Fragment：N-terminal domain
Mutation：C65A Non-standard monomer：Yes (specific site not provided by mmCIF) CL CHLORIDE ION × 1 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；277 K；PEG 6000, Ammonium Phosphate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 277.0K
Resolution 2.30 Å R-free 0.260
1P8V CRYSTAL STRUCTURE OF THE COMPLEX OF PLATELET RECEPTOR GPIB-ALPHA AND ALPHA-THROMBIN AT 2.6A Deposited 2003-05-07 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain A 17–294（278 aa） Fragment：Glycoprotein 1B alpha
Mutation：N21D Non-standard monomer：Yes (specific site not provided by mmCIF) MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 2 DFP DIISOPROPYL PHOSPHONATE × 2 X-RAY DIFFRACTION
X-ray crystallization conditions pH 6；291 K；14% PEG 400, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K, pH 6.00
Resolution 2.60 Å R-free 0.238
1P9A Crystal Structure of N-Terminal Domain of Human Platelet Receptor Glycoprotein Ib-alpha at 1.7 Angstrom Resolution Deposited 2003-05-09 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Monomer；Protein × 1 PDB declaration: monomeric
Chain G 17–306（290 aa） Fragment：N-Terminal Domain
Mutation：C65A No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 5.6；277 K；PEG 6000, Sodium Nitrate, Sodium Acetate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 1.70 Å R-free 0.224
1QYY Crystal Structure of N-Terminal Domain of Human Platelet Receptor Glycoprotein Ib-alpha at 2.8 Angstrom Resolution Deposited 2003-09-12 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Monomer；Protein × 1 PDB declaration: monomeric
Chain A 17–306（290 aa） Fragment：N-Terminal Domain
Mutation：C65A PT PLATINUM (II) ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 5.6；277 K；PEG 6000, SODIUM NITRATE, SODIUM ACETATE, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 2.80 Å R-free 0.282
1QYY Crystal Structure of N-Terminal Domain of Human Platelet Receptor Glycoprotein Ib-alpha at 2.8 Angstrom Resolution Deposited 2003-09-12 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain G 17–306（290 aa） Fragment：N-Terminal Domain
Mutation：C65A PT PLATINUM (II) ION × 1 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 5.6；277 K；PEG 6000, SODIUM NITRATE, SODIUM ACETATE, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 2.80 Å R-free 0.282
1SQ0 Crystal Structure of the Complex of the Wild-type Von Willebrand Factor A1 domain and Glycoprotein Ib alpha at 2.6 Angstrom Resolution Deposited 2004-03-17 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain B 17–304（288 aa） Fragment：Glycoprotein Ib-(alpha)
Mutation：N21D, N159D No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8；291 K；PEG-3350; potassium thiocyanate, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K
Resolution 2.60 Å R-free 0.239
1U0N The ternary von Willebrand Factor A1-glycoprotein Ibalpha-botrocetin complex Deposited 2004-07-13 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric
Chain D 17–281（265 aa） Fragment：Alpha chain
Mutation：N21Q,N159Q No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.5；277 K；Sodium citrate, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K
Resolution 2.95 Å R-free 0.276
2BP3 Crystal structure of Filamin A domain 17 and GPIb alpha cytoplasmic domain complex Deposited 2005-04-18 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain T 572–593（22 aa） Fragment：CYTOPLASMIC DOMAIN, RESIDUES 572-593
Not recorded GOL GLYCEROL × 1 X-RAY DIFFRACTION
X-ray crystallization conditions pH 8.2；1.75M AMMONIUM PHOSPHATE, PH 8.2, AFTER MICROSEEDING: 1.25M AMMONIUM SULFATE PH 8.2
Resolution 2.32 Å R-free 0.256
2BP3 Crystal structure of Filamin A domain 17 and GPIb alpha cytoplasmic domain complex Deposited 2005-04-18 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain S 572–593（22 aa） Fragment：CYTOPLASMIC DOMAIN, RESIDUES 572-593
Not recorded GOL GLYCEROL × 1 X-RAY DIFFRACTION
X-ray crystallization conditions pH 8.2；1.75M AMMONIUM PHOSPHATE, PH 8.2, AFTER MICROSEEDING: 1.25M AMMONIUM SULFATE PH 8.2
Resolution 2.32 Å R-free 0.256
3P72 structure of platelet Glycoprotein 1b alpha with a bound peptide inhibitor Deposited 2010-10-12 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 17–281（265 aa） Fragment：UNP residues 17-281
Not recorded CL CHLORIDE ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.2；298 K；1.9M ammonium sulfate, 80mM lithium sulfate, 100mM N-cyclohexyl-3-aminopropanesulfonic acid buffer. 1:1 mix buffer with 4mg/mL protein. lyophilised peptide added directly to crystallised protein drops., pH 8.2, VAPOR DIFFUSION, SITTING DROP, temperature 298K
Resolution 1.90 Å R-free 0.286
3PMH Mechanism of Sulfotyrosine-Mediated Glycoprotein Ib Interaction with Two Distinct alpha-Thrombin Sites Deposited 2010-11-16 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 3 PDB declaration: trimeric
Chain G 17–306（290 aa） Fragment：unp residues 17-306
Mutation：C65A Non-standard monomer：Yes (specific site not provided by mmCIF) 0G7 D-phenylalanyl-N-[(3S)-6-carbamimidamido-1-chloro-2-oxohexan-3-yl]-L-prolinamide × 1 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 1 X-RAY DIFFRACTION
X-ray crystallization conditions pH 7；277 K；16% PEG 6000, 0.2M ammonium phosphate, pH 7.0, VAPOR DIFFUSION, temperature 277K
Resolution 3.20 Å R-free 0.263
4C2A Crystal Structure of High-Affinity von Willebrand Factor A1 domain with R1306Q and I1309V Mutations in Complex with High Affinity GPIb alpha Deposited 2013-08-16 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain B 17–306（290 aa） Fragment：RESIDUES 17-306
Mutation：YES CAC CACODYLATE ION × 1 ACT ACETATE ION × 1 CA CALCIUM ION × 1 PEG DI(HYDROXYETHYL)ETHER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions pH 6.5；CRYSTALS OF A1/VWD2-GPIBALPA/VWD2 COMPLEX APPEARED IN 12% PEG 8000, 0.1 M SODIUM CACODYLATE PH 6.5, 0.1 M CALCIUM ACETATE, AND 0.4 MG/ML RISTOCETIN (SIGMA-ALDRICH). THESE CRYSTALS WERE CRUSHED AND USED FOR SEEDING. CRYSTALS GREW IN 10 MG/ML COMPLEX, 14% PEG8000, 0.2 M CALCIUM ACETATE, 0.1 M SODIUM CACODYLATE, PH 6.5, AND 0.4 MG/ML RISTOCETIN. NEXT WE SOAKED THESE CRYSTALS IN 14% PEG8000, 0.2 M CALCIUM ACETATE, 0.1 SODIUM CACODYLATE, PH 6.5, AND 4 MG/ML RISTOCETIN.
Resolution 2.08 Å R-free 0.189
4CH2 Low-salt crystal structure of a thrombin-GpIbalpha peptide complex Deposited 2013-11-28 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain P 287–300（14 aa） Fragment：C-TERMINAL FRAGMENT OF GPIBALPHA, 271-284
Non-standard monomer：Yes (specific site not provided by mmCIF) GOL GLYCEROL × 2 0G6 D-phenylalanyl-N-[(2S,3S)-6-{[amino(iminio)methyl]amino}-1-chloro-2-hydroxyhexan-3-yl]-L-prolinamide × 1 NA SODIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions pH 7.4；50 MM SODIUM FORMATE, 16% PEG-3350, pH 7.4
Resolution 1.60 Å R-free 0.187
4CH2 Low-salt crystal structure of a thrombin-GpIbalpha peptide complex Deposited 2013-11-28 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain Q 287–300（14 aa） Fragment：C-TERMINAL FRAGMENT OF GPIBALPHA, 271-284
Non-standard monomer：Yes (specific site not provided by mmCIF) GOL GLYCEROL × 2 0G6 D-phenylalanyl-N-[(2S,3S)-6-{[amino(iminio)methyl]amino}-1-chloro-2-hydroxyhexan-3-yl]-L-prolinamide × 1 NA SODIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions pH 7.4；50 MM SODIUM FORMATE, 16% PEG-3350, pH 7.4
Resolution 1.60 Å R-free 0.187
4CH8 High-salt crystal structure of a thrombin-GpIbalpha peptide complex Deposited 2013-11-29 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain P 287–300（14 aa） Fragment：C-TERMINAL FRAGMENT OF GPIBALPHA, RESIDUES 271-284
Non-standard monomer：Yes (specific site not provided by mmCIF) NA SODIUM ION × 1 0G6 D-phenylalanyl-N-[(2S,3S)-6-{[amino(iminio)methyl]amino}-1-chloro-2-hydroxyhexan-3-yl]-L-prolinamide × 1 GOL GLYCEROL × 1 X-RAY DIFFRACTION
X-ray crystallization conditions pH 7.4；100 MM SODIUM FORMATE, 18% PEG-3350, pH 7.4
Resolution 1.75 Å R-free 0.242
4CH8 High-salt crystal structure of a thrombin-GpIbalpha peptide complex Deposited 2013-11-29 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain Q 287–300（14 aa） Fragment：C-TERMINAL FRAGMENT OF GPIBALPHA, RESIDUES 271-284
Non-standard monomer：Yes (specific site not provided by mmCIF) NA SODIUM ION × 1 0G6 D-phenylalanyl-N-[(2S,3S)-6-{[amino(iminio)methyl]amino}-1-chloro-2-hydroxyhexan-3-yl]-L-prolinamide × 1 X-RAY DIFFRACTION
X-ray crystallization conditions pH 7.4；100 MM SODIUM FORMATE, 18% PEG-3350, pH 7.4
Resolution 1.75 Å R-free 0.242
4CH8 High-salt crystal structure of a thrombin-GpIbalpha peptide complex Deposited 2013-11-29 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 3 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain R 287–300（14 aa） Fragment：C-TERMINAL FRAGMENT OF GPIBALPHA, RESIDUES 271-284
Non-standard monomer：Yes (specific site not provided by mmCIF) NA SODIUM ION × 1 0G6 D-phenylalanyl-N-[(2S,3S)-6-{[amino(iminio)methyl]amino}-1-chloro-2-hydroxyhexan-3-yl]-L-prolinamide × 1 GOL GLYCEROL × 1 X-RAY DIFFRACTION
X-ray crystallization conditions pH 7.4；100 MM SODIUM FORMATE, 18% PEG-3350, pH 7.4
Resolution 1.75 Å R-free 0.242
4CH8 High-salt crystal structure of a thrombin-GpIbalpha peptide complex Deposited 2013-11-29 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 4 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain S 287–300（14 aa） Fragment：C-TERMINAL FRAGMENT OF GPIBALPHA, RESIDUES 271-284
Non-standard monomer：Yes (specific site not provided by mmCIF) NA SODIUM ION × 1 0G6 D-phenylalanyl-N-[(2S,3S)-6-{[amino(iminio)methyl]amino}-1-chloro-2-hydroxyhexan-3-yl]-L-prolinamide × 1 X-RAY DIFFRACTION
X-ray crystallization conditions pH 7.4；100 MM SODIUM FORMATE, 18% PEG-3350, pH 7.4
Resolution 1.75 Å R-free 0.242
4MGX Crystal structure of human filamin C domains 4-5 and GPIB alpha cytoplasmic domain complex Deposited 2013-08-29 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain B 577–585（9 aa） Fragment：cytoplasmic domain (UNP residues 577-585)
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；297 K；0.1 M HEPES, pH 7.5, 20% w/v PEG8000, VAPOR DIFFUSION, HANGING DROP, temperature 297K
Resolution 3.16 Å R-free 0.277
6XFQ Structure of a novel antithrombotic agent Agkisacucetin in complex with the platelet glycoprotein Ib receptor Deposited 2020-06-16 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain G 17–321（305 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；289 K；15% PEG4000, 0.1M HEPES, pH 7.5
Resolution 3.30 Å R-free 0.311
8WE2 14-3-3 zeta complexed with S609 phosphorylated peptide derived from GPIb alpha cytoplasmic domain Deposited 2023-09-16 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric
Chain Q 646–652（7 aa）
Chain R 646–652（7 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；291 K；0.2M Sodium Acetate, 20% PEG 3350
Resolution 2.11 Å R-free 0.238
8WF6 14-3-3 zeta complexed with S559 phosphorylated peptide derived from GPIb alpha cytoplasmic domain Deposited 2023-09-19 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric
Chain Q 596–607（12 aa）
Chain R 596–607（12 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；291 K；2% Tacsimate pH7.0, 0.1M Hepes pH7.5, 20% PEG 3350
Resolution 2.47 Å R-free 0.238
8WF6 14-3-3 zeta complexed with S559 phosphorylated peptide derived from GPIb alpha cytoplasmic domain Deposited 2023-09-19 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric
Chain E 596–607（12 aa）
Chain F 596–607（12 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) SIN SUCCINIC ACID × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；291 K；2% Tacsimate pH7.0, 0.1M Hepes pH7.5, 20% PEG 3350
Resolution 2.47 Å R-free 0.238
8WFS Cryo-EM structure of GPIb-IX Complex Deposited 2023-09-20 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 7 PDB declaration: heptameric
Chain a 523–652（130 aa）
Mutation：S559E, S609E No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen NITROGEN
Resolution 3.36 Å

20 other PDB entries and 29 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name GP1BA_HUMAN
Isoform —
PDB entities 2
Chains and sequence ranges Author chain B； PDBConstruct 1–290； UniProt 17–306 Author chain D； PDBConstruct 1–290； UniProt 17–306 Author chain F； PDBConstruct 1–290； UniProt 17–306 Author chain H； PDBConstruct 1–290； UniProt 17–306

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

SAXS scattering curve SAXS Profile

SAXS profile for 4c2b

P(r) Distance Distribution P(r) Distribution

P(r) distribution for 4c2b
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2. Structure Basics 2. Structure Basics

Entry ID entry_id4c2b
Deposition date deposition_date2013-08-16
Structure title titleCrystal Structure of High-Affinity von Willebrand Factor A1 domain with Disulfide Mutation in Complex with High Affinity GPIb alpha
Keywords keywordsBLOOD CLOTTING; BLOOD CLOTTING
Experimental Method methodX-RAY DIFFRACTION
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3. SAXS Parameters (CRYSOL theoretical calculation) 3. SAXS Parameters (CRYSOL)

Radius of gyration Rg (Guinier) rg_guinier43.94 Å
Radius of gyration Rg (electron density) rg_electron43.56 Å
Forward intensity I(0) i0603778000.00
Molecular weight molecular_weight209080.0 kDa
Excluded volume excluded_volume264980 ų
Envelope volume envelope_volume360310 ų
Hydration-shell volume shell_volume68533 ų
Envelope diameter envelope_diameter144.6 Å
Shell Rg shell_rg48.50 Å
Envelope Rg envelope_rg42.97 Å
Shape Rg shape_rg43.54 Å
Total Rg total_rg43.85 Å
Total atoms total_atoms14703
Residues n_residues1845
Spherical-harmonic order n_harmonics20
q range q_range— – 0.5000 Å−1
Data points n_points101
Shell type shell_typedirectional
Solvent electron density solvent_density0.3340 e/ų
Shell contrast contrast_shell0.0300 e/ų
CRYSOL version crysol_version4.1.3
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4. P(r) Distance Distribution (GNOM inversion) 4. P(r) Analysis (GNOM)

Maximum dimension Dmax dmax138.9 Å
Rg (real space) rg_real43.82 Å
Rg uncertainty (real space) rg_real_error0.88 Å
I(0) (real space) i0_real6.0380e+08
I(0) uncertainty (real space) i0_real_error1.0630e+07
Rg (reciprocal space) rg_reciprocal43.94 Å
I(0) (reciprocal space) i0_reciprocal603900000.0000
Solution quality estimate total_estimate0.8677
Solution quality rating solution_quality GOOD a GOOD solution
P(r) peaks n_peaks1
Primary peak position r_peak_primary57.9 Å
Skewness Skewness skewness0.204
Kurtosis Kurtosis kurtosis-0.446
Angular range angular_range— – 0.1800 Å−1
Current regularization parameter α current_alpha0.0000
Highest regularization parameter α highest_alpha107400000.0000
Real-space data points n_real_points37
GNOM version gnom_version4.1.3
Quality Criteria quality_criteria AN1: 0.000; Oscil: 0.926; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.998; Smooth: 0.500
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5. Crystallography and Experiment 5. Crystallography & Experiment

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6. Entities and Polymers Entities & Polymers (6)

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7. Fold Classification (SCOP + CATH) 16 domains

SCOP 2.08 (8 domains)

Domain ID domain_idd4c2ba_
Class classc — Alpha and beta proteins (a/b)
Fold Fold foldc.62 — vWA-like
Superfamily Superfamily superfamilyc.62.1 — vWA-like
Family Family familyc.62.1.1 — Integrin A (or I) domain
Domain ID domain_idd4c2bb_
Class classc — Alpha and beta proteins (a/b)
Fold Fold foldc.10 — Leucine-rich repeat, LRR (right-handed beta-alpha superhelix)
Superfamily Superfamily superfamilyc.10.2 — L domain-like
Family Family familyc.10.2.7 — Ngr ectodomain-like
Domain ID domain_idd4c2bc_
Class classc — Alpha and beta proteins (a/b)
Fold Fold foldc.62 — vWA-like
Superfamily Superfamily superfamilyc.62.1 — vWA-like
Family Family familyc.62.1.1 — Integrin A (or I) domain
Domain ID domain_idd4c2bd_
Class classc — Alpha and beta proteins (a/b)
Fold Fold foldc.10 — Leucine-rich repeat, LRR (right-handed beta-alpha superhelix)
Superfamily Superfamily superfamilyc.10.2 — L domain-like
Family Family familyc.10.2.7 — Ngr ectodomain-like
Domain ID domain_idd4c2be_
Class classc — Alpha and beta proteins (a/b)
Fold Fold foldc.62 — vWA-like
Superfamily Superfamily superfamilyc.62.1 — vWA-like
Family Family familyc.62.1.1 — Integrin A (or I) domain
Domain ID domain_idd4c2bf_
Class classc — Alpha and beta proteins (a/b)
Fold Fold foldc.10 — Leucine-rich repeat, LRR (right-handed beta-alpha superhelix)
Superfamily Superfamily superfamilyc.10.2 — L domain-like
Family Family familyc.10.2.7 — Ngr ectodomain-like
Domain ID domain_idd4c2bg_
Class classc — Alpha and beta proteins (a/b)
Fold Fold foldc.62 — vWA-like
Superfamily Superfamily superfamilyc.62.1 — vWA-like
Family Family familyc.62.1.1 — Integrin A (or I) domain
Domain ID domain_idd4c2bh_
Class classc — Alpha and beta proteins (a/b)
Fold Fold foldc.10 — Leucine-rich repeat, LRR (right-handed beta-alpha superhelix)
Superfamily Superfamily superfamilyc.10.2 — L domain-like
Family Family familyc.10.2.7 — Ngr ectodomain-like

CATH v4.4 (8 domains)

Domain ID domain_id4c2bA00
Class class3 — Alpha Beta
Architecture architecture40 — 3-Layer(aba) Sandwich
Topology topology50 — Rossmann fold
Homologous superfamily homologous superfamily410 — von Willebrand factor, type A domain
Domain ID domain_id4c2bB00
Class class3 — Alpha Beta
Architecture architecture80 — Alpha-Beta Horseshoe
Topology topology10 — Leucine-rich repeat, LRR (right-handed beta-alpha superhelix)
Homologous superfamily homologous superfamily10 — Ribonuclease Inhibitor
Domain ID domain_id4c2bC00
Class class3 — Alpha Beta
Architecture architecture40 — 3-Layer(aba) Sandwich
Topology topology50 — Rossmann fold
Homologous superfamily homologous superfamily410 — von Willebrand factor, type A domain
Domain ID domain_id4c2bD00
Class class3 — Alpha Beta
Architecture architecture80 — Alpha-Beta Horseshoe
Topology topology10 — Leucine-rich repeat, LRR (right-handed beta-alpha superhelix)
Homologous superfamily homologous superfamily10 — Ribonuclease Inhibitor
Domain ID domain_id4c2bE00
Class class3 — Alpha Beta
Architecture architecture40 — 3-Layer(aba) Sandwich
Topology topology50 — Rossmann fold
Homologous superfamily homologous superfamily410 — von Willebrand factor, type A domain
Domain ID domain_id4c2bF00
Class class3 — Alpha Beta
Architecture architecture80 — Alpha-Beta Horseshoe
Topology topology10 — Leucine-rich repeat, LRR (right-handed beta-alpha superhelix)
Homologous superfamily homologous superfamily10 — Ribonuclease Inhibitor
Domain ID domain_id4c2bG00
Class class3 — Alpha Beta
Architecture architecture40 — 3-Layer(aba) Sandwich
Topology topology50 — Rossmann fold
Homologous superfamily homologous superfamily410 — von Willebrand factor, type A domain
Domain ID domain_id4c2bH00
Class class3 — Alpha Beta
Architecture architecture80 — Alpha-Beta Horseshoe
Topology topology10 — Leucine-rich repeat, LRR (right-handed beta-alpha superhelix)
Homologous superfamily homologous superfamily10 — Ribonuclease Inhibitor
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8. Citations (1)

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9. Files and Curves (10)