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8iyk

Tail tip conformation 1 of phage lambda tail

Method: ELECTRON MICROSCOPY Dmax: 287.9 Å Quality: GOOD
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1. Protein Identity and Related Structures Protein Identity & Related Structures

Tail tube protein

Escherichia phage lambda

UniProt P03733

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 42 PDB declaration: 42-meric（42） Consistent with protein copy count Chain A； UniProt 1–246 Chain B； UniProt 1–246 Chain C； UniProt 1–246 Chain D； UniProt 1–246 Chain G； UniProt 1–246 Chain Q； UniProt 1–246 Chain R； UniProt 1–246 Chain S； UniProt 1–246 Chain T； UniProt 1–246 Chain U； UniProt 1–246 Chain V； UniProt 1–246 Chain W； UniProt 1–246 Chain X； UniProt 1–246 Chain a； UniProt 1–246 Chain b； UniProt 1–246 Chain c； UniProt 1–246 Chain d； UniProt 1–246 Chain i； UniProt 1–246 Chain j； UniProt 1–246 Chain k； UniProt 1–246 Chain l； UniProt 1–246 Chain n； UniProt 1–246 Chain o； UniProt 1–246 Chain v； UniProt 1–246 Not recorded Tail tip protein M × 6 （P03737） Tip attachment protein J × 3 （P03749） Tail tip protein L × 3 （P03738） Tape measure protein × 3 （P03736） Tail tip assembly protein I × 3 （P03730） SF4 IRON/SULFUR CLUSTER × 3 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.5 cryo-EM vitrification conditions：Cryogen NITROGEN Resolution 2.95 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 8IYK Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
2K4Q The Solution Structure of gpV, the Major Tail Protein from Bacteriophage Lambda Deposited 2008-06-16 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 1–153（153 aa）
Not recorded No recorded non-water small molecule SOLUTION NMR
NMR measurement conditions pH 6.8；293 K；Ionic strength (raw mmCIF value) 200；Pressure ambient
NMR sample composition 50 mM sodium phosphate, 0.6 mM DSS, 200 mM sodium chloride, 8-10 % D2O, 0.1% % sodium azide, 1 mM [U-100% 13C; U-100% 15N] gpV protein, 90% H2O/10% D2O | 90% H2O/10% D2O
NMR sample composition 1 mM [U-100% 13C; U-100% 15N] gpV protein, 90-99 % D2O, 0.6 mM DSS, 50 mM sodium phosphate, 200 mM sodium chloride, 0.1 % sodium azide, 90% D2O, 10% H2O | 90% D2O, 10% H2O
Resolution not provided
2L04 The Solution Structure of the C-terminal Ig-like Domain of the Bacteriophage Lambda Tail Tube Protein Deposited 2010-06-30 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 160–246（87 aa） Fragment：C-terminal, UNP residues 160-246
Not recorded No recorded non-water small molecule SOLUTION NMR
NMR measurement conditions pH 6.8；298 K；Ionic strength (raw mmCIF value) .2；Pressure ambient
NMR sample composition 50 mM sodium phosphate-1, 200 mM sodium chloride-2, 90% H2O/10% D2O | 90% H2O/10% D2O
NMR sample composition 50 mM sodium phosphate-3, 200 mM sodium chloride-4, 100% D2O | 100% D2O
Resolution not provided
6P3E Mobile loops and electrostatic interactions maintain the flexible lambda tail tube Deposited 2019-05-23 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 18 PDB declaration: octadecameric
Chain A 1–246（246 aa）
Chain B 1–246（246 aa）
Chain C 1–246（246 aa）
Chain D 1–246（246 aa）
Chain E 1–246（246 aa）
Chain F 1–246（246 aa）
Chain G 1–246（246 aa）
Chain H 1–246（246 aa）
Chain I 1–246（246 aa）
Chain J 1–246（246 aa）
Chain K 1–246（246 aa）
Chain L 1–246（246 aa）
Chain M 1–246（246 aa）
Chain N 1–246（246 aa）
Chain O 1–246（246 aa）
Chain P 1–246（246 aa）
Chain Q 1–246（246 aa）
Chain R 1–246（246 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 5.40 Å
7T2E Structure of bacteriophage lambda tube protein V in C6 Deposited 2021-12-03 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 2–157（156 aa）
Chain B 2–157（156 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.70 Å
7T4F Structure of bacteriophage lambda tube protein V in C3 Deposited 2021-12-09 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 2–246（245 aa）
Chain B 2–246（245 aa）
Chain C 2–246（245 aa）
Chain D 2–246（245 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.70 Å
8IYD Tail cap of phage lambda tail Deposited 2023-04-04 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 30 PDB declaration: 30-meric
Chain A 1–246（246 aa）
Chain B 1–246（246 aa）
Chain C 1–246（246 aa）
Chain D 1–246（246 aa）
Chain E 1–246（246 aa）
Chain F 1–246（246 aa）
Chain H 1–246（246 aa）
Chain I 1–246（246 aa）
Chain J 1–246（246 aa）
Chain L 1–246（246 aa）
Chain M 1–246（246 aa）
Chain N 1–246（246 aa）
Chain O 1–246（246 aa）
Chain P 1–246（246 aa）
Chain R 1–246（246 aa）
Chain S 1–246（246 aa）
Chain T 1–246（246 aa）
Chain V 1–246（246 aa）
Chain X 1–246（246 aa）
Chain Y 1–246（246 aa）
Chain a 1–246（246 aa）
Chain b 1–246（246 aa）
Chain c 1–246（246 aa）
Chain v 1–246（246 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen NITROGEN
Resolution 3.10 Å
8IYL Tail tip conformation 2 of phage lambda tail Deposited 2023-04-05 Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 42 PDB declaration: 42-meric
Chain A 1–246（246 aa）
Chain B 1–246（246 aa）
Chain C 1–246（246 aa）
Chain O 1–246（246 aa）
Chain P 1–246（246 aa）
Chain Q 1–246（246 aa）
Chain R 1–246（246 aa）
Chain S 1–246（246 aa）
Chain T 1–246（246 aa）
Chain U 1–246（246 aa）
Chain V 1–246（246 aa）
Chain W 1–246（246 aa）
Chain a 1–246（246 aa）
Chain b 1–246（246 aa）
Chain c 1–246（246 aa）
Chain g 1–246（246 aa）
Chain h 1–246（246 aa）
Chain i 1–246（246 aa）
Chain j 1–246（246 aa）
Chain k 1–246（246 aa）
Chain l 1–246（246 aa）
Chain n 1–246（246 aa）
Chain o 1–246（246 aa）
Chain v 1–246（246 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 3 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen NITROGEN
Resolution 3.00 Å
8K35 Structure of the bacteriophage lambda tail tip complex Deposited 2023-07-14 Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 24 PDB declaration: 24-meric
Chain L 1–246（246 aa）
Chain R 1–246（246 aa）
Chain T 1–246（246 aa）
Chain U 1–246（246 aa）
Chain V 1–246（246 aa）
Chain W 1–246（246 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 3 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.44 Å
8K36 Structure of the bacteriophage lambda tail tube Deposited 2023-07-14 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 12 PDB declaration: dodecameric
Chain A 1–246（246 aa）
Chain B 1–246（246 aa）
Chain C 1–246（246 aa）
Chain D 1–246（246 aa）
Chain E 1–246（246 aa）
Chain F 1–246（246 aa）
Chain G 1–246（246 aa）
Chain H 1–246（246 aa）
Chain I 1–246（246 aa）
Chain J 1–246（246 aa）
Chain K 1–246（246 aa）
Chain L 1–246（246 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.48 Å
8K37 Structure of the bacteriophage lambda neck Deposited 2023-07-14 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 18 PDB declaration: octadecameric
Chain G 1–246（246 aa）
Chain H 1–246（246 aa）
Chain I 1–246（246 aa）
Chain J 1–246（246 aa）
Chain K 1–246（246 aa）
Chain L 1–246（246 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.50 Å
8KGE Dimeric tail tube protein gpVs of bacteriophage lambda Deposited 2023-08-18 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain V 1–246（246 aa）
Chain v 1–246（246 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen NITROGEN
Resolution 3.80 Å
8XPM Mature virion portal of phage lambda with DNA Deposited 2024-01-04 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–DNA Heteromer；Protein × 66 PDB declaration: 68-meric
Chain O 1–246（246 aa）
Chain O1 1–246（246 aa）
Chain O2 1–246（246 aa）
Chain P 1–246（246 aa）
Chain P1 1–246（246 aa）
Chain P2 1–246（246 aa）
Chain Q 1–246（246 aa）
Chain Q1 1–246（246 aa）
Chain Q2 1–246（246 aa）
Chain R 1–246（246 aa）
Chain R1 1–246（246 aa）
Chain R2 1–246（246 aa）
Chain V 1–246（246 aa）
Chain V1 1–246（246 aa）
Chain V2 1–246（246 aa）
Chain o 1–246（246 aa）
Chain o1 1–246（246 aa）
Chain o2 1–246（246 aa）
Chain p 1–246（246 aa）
Chain p1 1–246（246 aa）
Chain p2 1–246（246 aa）
Chain q 1–246（246 aa）
Chain q1 1–246（246 aa）
Chain q2 1–246（246 aa）
Chain r 1–246（246 aa）
Chain r1 1–246（246 aa）
Chain r2 1–246（246 aa）
Chain v 1–246（246 aa）
Chain v1 1–246（246 aa）
Chain v2 1–246（246 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen NITROGEN
Resolution 3.90 Å
9E7M In situ cryoEM structure of bacteriophage Ur-lambda tail tip complex Deposited 2024-11-03 Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 36 PDB declaration: 36-meric
Chain Va 3–157（155 aa）
Chain Vb 3–157（155 aa）
Chain Vc 3–157（155 aa）
Chain Vd 3–157（155 aa）
Chain Ve 3–157（155 aa）
Chain Vf 3–157（155 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 3 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.37 Å

13 other PDB entries and 13 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name TUBE_LAMBD
Isoform —
PDB entities 1
Chains and sequence ranges Author chain A； PDBConstruct 1–246； UniProt 1–246 Author chain B； PDBConstruct 1–246； UniProt 1–246 Author chain C； PDBConstruct 1–246； UniProt 1–246 Author chain D； PDBConstruct 1–246； UniProt 1–246 Author chain G； PDBConstruct 1–246； UniProt 1–246 Author chain Q； PDBConstruct 1–246； UniProt 1–246 Author chain R； PDBConstruct 1–246； UniProt 1–246 Author chain S； PDBConstruct 1–246； UniProt 1–246 Author chain T； PDBConstruct 1–246； UniProt 1–246 Author chain U； PDBConstruct 1–246； UniProt 1–246 Author chain V； PDBConstruct 1–246； UniProt 1–246 Author chain W； PDBConstruct 1–246； UniProt 1–246 Author chain X； PDBConstruct 1–246； UniProt 1–246 Author chain a； PDBConstruct 1–246； UniProt 1–246 Author chain b； PDBConstruct 1–246； UniProt 1–246 Author chain c； PDBConstruct 1–246； UniProt 1–246 Author chain d； PDBConstruct 1–246； UniProt 1–246 Author chain i； PDBConstruct 1–246； UniProt 1–246 Author chain j； PDBConstruct 1–246； UniProt 1–246 Author chain k； PDBConstruct 1–246； UniProt 1–246 Author chain l； PDBConstruct 1–246； UniProt 1–246 Author chain n； PDBConstruct 1–246； UniProt 1–246 Author chain o； PDBConstruct 1–246； UniProt 1–246 Author chain v； PDBConstruct 1–246； UniProt 1–246

Tail tip protein M

Escherichia phage lambda

UniProt P03737

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 42 PDB declaration: 42-meric（42） Consistent with protein copy count Chain E； UniProt 1–109 Chain K； UniProt 1–109 Chain M； UniProt 1–109 Chain Y； UniProt 1–109 Chain e； UniProt 1–109 Chain m； UniProt 1–109 Not recorded Tail tube protein × 24 （P03733） Tip attachment protein J × 3 （P03749） Tail tip protein L × 3 （P03738） Tape measure protein × 3 （P03736） Tail tip assembly protein I × 3 （P03730） SF4 IRON/SULFUR CLUSTER × 3 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.5 cryo-EM vitrification conditions：Cryogen NITROGEN Resolution 2.95 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 8IYK Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
8IYL Tail tip conformation 2 of phage lambda tail Deposited 2023-04-05 Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 42 PDB declaration: 42-meric
Chain D 1–109（109 aa）
Chain F 1–109（109 aa）
Chain M 1–109（109 aa）
Chain X 1–109（109 aa）
Chain Z 1–109（109 aa）
Chain m 1–109（109 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 3 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen NITROGEN
Resolution 3.00 Å
8K35 Structure of the bacteriophage lambda tail tip complex Deposited 2023-07-14 Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 24 PDB declaration: 24-meric
Chain C 1–109（109 aa）
Chain D 1–109（109 aa）
Chain E 1–109（109 aa）
Chain F 1–109（109 aa）
Chain H 1–109（109 aa）
Chain O 1–109（109 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 3 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.44 Å
8XCG Tail tip complex of bacteriophage lambda in the open state Deposited 2023-12-09 Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 15 PDB declaration: pentadecameric
Chain E 1–109（109 aa）
Chain K 1–109（109 aa）
Chain M 1–109（109 aa）
Chain Y 1–109（109 aa）
Chain e 1–109（109 aa）
Chain m 1–109（109 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 3 ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.46 Å
9E7M In situ cryoEM structure of bacteriophage Ur-lambda tail tip complex Deposited 2024-11-03 Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 36 PDB declaration: 36-meric
Chain Ma 1–109（109 aa）
Chain Mb 1–109（109 aa）
Chain Mc 1–109（109 aa）
Chain Md 1–109（109 aa）
Chain Me 1–109（109 aa）
Chain Mf 1–109（109 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 3 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.37 Å

4 other PDB entries and 4 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name TIPM_LAMBD
Isoform —
PDB entities 2
Chains and sequence ranges Author chain E； PDBConstruct 1–109； UniProt 1–109 Author chain K； PDBConstruct 1–109； UniProt 1–109 Author chain M； PDBConstruct 1–109； UniProt 1–109 Author chain Y； PDBConstruct 1–109； UniProt 1–109 Author chain e； PDBConstruct 1–109； UniProt 1–109 Author chain m； PDBConstruct 1–109； UniProt 1–109

Tip attachment protein J

Escherichia phage lambda

UniProt P03749

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 42 PDB declaration: 42-meric（42） Consistent with protein copy count Chain F； UniProt 1–1132 Chain J； UniProt 1–1132 Chain Z； UniProt 1–1132 Not recorded Tail tube protein × 24 （P03733） Tail tip protein M × 6 （P03737） Tail tip protein L × 3 （P03738） Tape measure protein × 3 （P03736） Tail tip assembly protein I × 3 （P03730） SF4 IRON/SULFUR CLUSTER × 3 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.5 cryo-EM vitrification conditions：Cryogen NITROGEN Resolution 2.95 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 8IYK Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
8IYL Tail tip conformation 2 of phage lambda tail Deposited 2023-04-05 Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 42 PDB declaration: 42-meric
Chain E 1–1132（1132 aa）
Chain J 1–1132（1132 aa）
Chain Y 1–1132（1132 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 3 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen NITROGEN
Resolution 3.00 Å
8JVM AHS-CSF domains of phage lambda tail Deposited 2023-06-28 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric
Chain F 830–995（166 aa） Fragment：AHS-CSF domains
Chain J 830–995（166 aa） Fragment：AHS-CSF domains
Chain Z 830–995（166 aa） Fragment：AHS-CSF domains
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen NITROGEN
Resolution 3.86 Å
8K35 Structure of the bacteriophage lambda tail tip complex Deposited 2023-07-14 Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 24 PDB declaration: 24-meric
Chain A 1–1132（1132 aa）
Chain B 1–1132（1132 aa）
Chain I 1–1132（1132 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 3 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.44 Å
8XCG Tail tip complex of bacteriophage lambda in the open state Deposited 2023-12-09 Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 15 PDB declaration: pentadecameric
Chain F 1–1132（1132 aa）
Chain J 1–1132（1132 aa）
Chain Z 1–1132（1132 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 3 ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.46 Å
8XCI Open state of central tail fiber of bacteriophage lambda upon binding to LamB Deposited 2023-12-09 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric
Chain F 1–1132（1132 aa）
Chain J 1–1132（1132 aa）
Chain Z 1–1132（1132 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.57 Å
8XCJ Open State of central tail fiber of bacteriophage lambda upon binding to LamB (gpJ713-LamB complex) Deposited 2023-12-09 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain F 713–1132（420 aa）
Chain J 713–1132（420 aa）
Chain Z 713–1132（420 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.98 Å
8XCK Closed state of central tail fiber of bacteriophage lambda Deposited 2023-12-09 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain F 713–1132（420 aa）
Chain J 713–1132（420 aa）
Chain Z 713–1132（420 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.75 Å
9E7M In situ cryoEM structure of bacteriophage Ur-lambda tail tip complex Deposited 2024-11-03 Different construct Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 36 PDB declaration: 36-meric
Chain Ja 1–835（835 aa）
Chain Jb 1–835（835 aa）
Chain Jc 1–835（835 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 3 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.37 Å
9OIK Structure of S. Typhimurium 14028 Gifsy-1 prophage HepS bound to bacteriophage lambda J Tail Tip Deposited 2025-05-06 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 8 PDB declaration: octameric
Chain C 902–933（32 aa）
Chain D 902–933（32 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；291 K；0.2 M Sodium Acetate, 16% PEG 4000, 0.1 M Tris pH 8.5
Resolution 1.86 Å R-free 0.209

9 other PDB entries and 9 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name TIPJ_LAMBD
Isoform —
PDB entities 3
Chains and sequence ranges Author chain F； PDBConstruct 1–1132； UniProt 1–1132 Author chain J； PDBConstruct 1–1132； UniProt 1–1132 Author chain Z； PDBConstruct 1–1132； UniProt 1–1132

Tail tip protein L

Escherichia phage lambda

UniProt P03738

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 42 PDB declaration: 42-meric（42） Consistent with protein copy count Chain L； UniProt 1–232 Chain N； UniProt 1–232 Chain f； UniProt 1–232 Not recorded Tail tube protein × 24 （P03733） Tail tip protein M × 6 （P03737） Tip attachment protein J × 3 （P03749） Tape measure protein × 3 （P03736） Tail tip assembly protein I × 3 （P03730） SF4 IRON/SULFUR CLUSTER × 3 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.5 cryo-EM vitrification conditions：Cryogen NITROGEN Resolution 2.95 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 8IYK Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
8IYL Tail tip conformation 2 of phage lambda tail Deposited 2023-04-05 Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 42 PDB declaration: 42-meric
Chain G 1–232（232 aa）
Chain L 1–232（232 aa）
Chain d 1–232（232 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 3 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen NITROGEN
Resolution 3.00 Å
8K35 Structure of the bacteriophage lambda tail tip complex Deposited 2023-07-14 Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 24 PDB declaration: 24-meric
Chain G 1–232（232 aa）
Chain J 1–232（232 aa）
Chain N 1–232（232 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 3 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.44 Å
8XCG Tail tip complex of bacteriophage lambda in the open state Deposited 2023-12-09 Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 15 PDB declaration: pentadecameric
Chain L 1–232（232 aa）
Chain N 1–232（232 aa）
Chain f 1–232（232 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 3 ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.46 Å
9E7M In situ cryoEM structure of bacteriophage Ur-lambda tail tip complex Deposited 2024-11-03 Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 36 PDB declaration: 36-meric
Chain La 1–232（232 aa）
Chain Lb 1–232（232 aa）
Chain Lc 1–232（232 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 3 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.37 Å

4 other PDB entries and 4 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name TIPL_LAMBD
Isoform —
PDB entities 4
Chains and sequence ranges Author chain L； PDBConstruct 1–232； UniProt 1–232 Author chain N； PDBConstruct 1–232； UniProt 1–232 Author chain f； PDBConstruct 1–232； UniProt 1–232

Tape measure protein

Escherichia phage lambda

UniProt P03736

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 42 PDB declaration: 42-meric（42） Consistent with protein copy count Chain H； UniProt 1–853 Chain O； UniProt 1–853 Chain g； UniProt 1–853 Not recorded Tail tube protein × 24 （P03733） Tail tip protein M × 6 （P03737） Tip attachment protein J × 3 （P03749） Tail tip protein L × 3 （P03738） Tail tip assembly protein I × 3 （P03730） SF4 IRON/SULFUR CLUSTER × 3 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.5 cryo-EM vitrification conditions：Cryogen NITROGEN Resolution 2.95 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 8IYK Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
8IYL Tail tip conformation 2 of phage lambda tail Deposited 2023-04-05 Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 42 PDB declaration: 42-meric
Chain H 1–853（853 aa）
Chain K 1–853（853 aa）
Chain e 1–853（853 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 3 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen NITROGEN
Resolution 3.00 Å
8K35 Structure of the bacteriophage lambda tail tip complex Deposited 2023-07-14 Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 24 PDB declaration: 24-meric
Chain M 1–853（853 aa）
Chain P 1–853（853 aa）
Chain S 1–853（853 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 3 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.44 Å
9E7M In situ cryoEM structure of bacteriophage Ur-lambda tail tip complex Deposited 2024-11-03 Different construct Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 36 PDB declaration: 36-meric
Chain Ha 818–849（32 aa）
Chain Hb 818–849（32 aa）
Chain Hc 818–849（32 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 3 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.37 Å

3 other PDB entries and 3 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name TMP_LAMBD
Isoform —
PDB entities 5
Chains and sequence ranges Author chain H； PDBConstruct 1–853； UniProt 1–853 Author chain O； PDBConstruct 1–853； UniProt 1–853 Author chain g； PDBConstruct 1–853； UniProt 1–853

Tail tip assembly protein I

Escherichia phage lambda

UniProt P03730

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 42 PDB declaration: 42-meric（42） Consistent with protein copy count Chain I； UniProt 1–223 Chain P； UniProt 1–223 Chain h； UniProt 1–223 Not recorded Tail tube protein × 24 （P03733） Tail tip protein M × 6 （P03737） Tip attachment protein J × 3 （P03749） Tail tip protein L × 3 （P03738） Tape measure protein × 3 （P03736） SF4 IRON/SULFUR CLUSTER × 3 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.5 cryo-EM vitrification conditions：Cryogen NITROGEN Resolution 2.95 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 8IYK Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
8IYL Tail tip conformation 2 of phage lambda tail Deposited 2023-04-05 Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 42 PDB declaration: 42-meric
Chain I 1–223（223 aa）
Chain N 1–223（223 aa）
Chain f 1–223（223 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 3 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen NITROGEN
Resolution 3.00 Å
8K35 Structure of the bacteriophage lambda tail tip complex Deposited 2023-07-14 Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 24 PDB declaration: 24-meric
Chain K 1–223（223 aa）
Chain Q 1–223（223 aa）
Chain x 1–223（223 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 3 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.44 Å
8XCG Tail tip complex of bacteriophage lambda in the open state Deposited 2023-12-09 Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 15 PDB declaration: pentadecameric
Chain I 1–223（223 aa）
Chain P 1–223（223 aa）
Chain h 1–223（223 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 3 ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.46 Å
9E7M In situ cryoEM structure of bacteriophage Ur-lambda tail tip complex Deposited 2024-11-03 Different construct Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 36 PDB declaration: 36-meric
Chain Ia 133–222（90 aa）
Chain Ib 133–222（90 aa）
Chain Ic 133–222（90 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 3 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.37 Å

4 other PDB entries and 4 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name TIPI_LAMBD
Isoform —
PDB entities 6
Chains and sequence ranges Author chain I； PDBConstruct 1–223； UniProt 1–223 Author chain P； PDBConstruct 1–223； UniProt 1–223 Author chain h； PDBConstruct 1–223； UniProt 1–223

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

SAXS scattering curve SAXS Profile

SAXS profile for 8iyk

P(r) Distance Distribution P(r) Distribution

P(r) distribution for 8iyk
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2. Structure Basics 2. Structure Basics

Entry ID entry_id8iyk
Deposition date deposition_date2023-04-05
Structure title titleTail tip conformation 1 of phage lambda tail
Keywords keywords;Bacteriophage, caudovirales, siphoviridae, tail complex, delivery device, macromolecular assembly, phage lambda, cryo-EM, VIRAL PROTEIN ;; VIRAL PROTEIN
Experimental Method methodELECTRON MICROSCOPY
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3. SAXS Parameters (CRYSOL theoretical calculation) 3. SAXS Parameters (CRYSOL)

Radius of gyration Rg (Guinier) rg_guinier— Å
Radius of gyration Rg (electron density) rg_electron105.90 Å
Forward intensity I(0) i017298300000.00
Molecular weight molecular_weight1099800.0 kDa
Excluded volume excluded_volume1368200 ų
Envelope volume envelope_volume2328400 ų
Hydration-shell volume shell_volume206650 ų
Envelope diameter envelope_diameter443.4 Å
Shell Rg shell_rg75.89 Å
Envelope Rg envelope_rg105.60 Å
Shape Rg shape_rg105.90 Å
Total Rg total_rg105.60 Å
Total atoms total_atoms77370
Residues n_residues10248
Spherical-harmonic order n_harmonics20
q range q_range— – 0.5000 Å−1
Data points n_points101
Shell type shell_typedirectional
Solvent electron density solvent_density0.3340 e/ų
Shell contrast contrast_shell0.0300 e/ų
CRYSOL version crysol_version4.1.3
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4. P(r) Distance Distribution (GNOM inversion) 4. P(r) Analysis (GNOM)

Maximum dimension Dmax dmax287.9 Å
Rg (real space) rg_real96.06 Å
Rg uncertainty (real space) rg_real_error1.57 Å
I(0) (real space) i0_real1.6520e+10
I(0) uncertainty (real space) i0_real_error3.5250e+08
Rg (reciprocal space) rg_reciprocal92.28 Å
I(0) (reciprocal space) i0_reciprocal16730000000.0000
Solution quality estimate total_estimate0.8932
Solution quality rating solution_quality GOOD a GOOD solution
P(r) peaks n_peaks1
Primary peak position r_peak_primary76.8 Å
Skewness Skewness skewness0.475
Kurtosis Kurtosis kurtosis-0.635
Angular range angular_range— – 0.0750 Å−1
Current regularization parameter α current_alpha0.8932
Highest regularization parameter α highest_alpha1368000000.0000
Real-space data points n_real_points16
GNOM version gnom_version4.1.3
Quality Criteria quality_criteria AN1: 0.003; Oscil: 0.893; Stabil: 0.976; Sysdev: 1.000; Positv: 1.000; Valcen: 1.000; Smooth: 0.005
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5. Crystallography and Experiment 5. Crystallography & Experiment

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6. Entities and Polymers Entities & Polymers (7)

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8. Citations (1)

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9. Files and Curves (10)