8uy3

Fem1B with FNIP1 and Tom20 fragment

Method: X-RAY DIFFRACTION

▼

1. Protein Identity and Related Structures Protein Identity & Related Structures

Protein fem-1 homolog B

Mus musculus

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein heterocomplex	Heteromer	Protein 3	Folliculin-interacting protein 1 × 1 （Q68FD7） Mitochondrial import receptor subunit TOM20 homolog × 1 （Q15388） ZINC ION × 2 4-(2-HYDROXYETHYL)-1-PIPERAZINE ETHANESULFONIC ACID × 1 PHOSPHATE ION × 1	Consistent with protein count
2	Protein heterocomplex	Heteromer	Protein 2	Folliculin-interacting protein 1 × 1 （Q68FD7） ZINC ION × 2 SULFATE ION × 1	Consistent with protein count
3	Protein heterocomplex	Heteromer	Protein 3	Folliculin-interacting protein 1 × 1 （Q68FD7） Mitochondrial import receptor subunit TOM20 homolog × 1 （Q15388） ZINC ION × 2 SULFATE ION × 1	Consistent with protein count
4	Protein heterocomplex	Heteromer	Protein 3	Folliculin-interacting protein 1 × 1 （Q68FD7） Mitochondrial import receptor subunit TOM20 homolog × 1 （Q15388） ZINC ION × 2	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	FEM1B_MOUSE
Isoform	—
PDB entities	1
Chains and sequence ranges	Author chain A； PDBConstruct 5–381； UniProt 1–377 Author chain B； PDBConstruct 5–381； UniProt 1–377 Author chain C； PDBConstruct 5–381； UniProt 1–377 Author chain D； PDBConstruct 5–381； UniProt 1–377

Folliculin-interacting protein 1

Mus musculus

UniProt Q68FD7

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein heterocomplex	Heteromer	Protein 3	Protein fem-1 homolog B × 1 （Q9Z2G0） Mitochondrial import receptor subunit TOM20 homolog × 1 （Q15388） ZINC ION × 2 4-(2-HYDROXYETHYL)-1-PIPERAZINE ETHANESULFONIC ACID × 1 PHOSPHATE ION × 1	Consistent with protein count
2	Protein heterocomplex	Heteromer	Protein 2	Protein fem-1 homolog B × 1 （Q9Z2G0） ZINC ION × 2 SULFATE ION × 1	Consistent with protein count
3	Protein heterocomplex	Heteromer	Protein 3	Protein fem-1 homolog B × 1 （Q9Z2G0） Mitochondrial import receptor subunit TOM20 homolog × 1 （Q15388） ZINC ION × 2 SULFATE ION × 1	Consistent with protein count
4	Protein heterocomplex	Heteromer	Protein 3	Protein fem-1 homolog B × 1 （Q9Z2G0） Mitochondrial import receptor subunit TOM20 homolog × 1 （Q15388） ZINC ION × 2	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	FNIP1_MOUSE
Isoform	—
PDB entities	2
Chains and sequence ranges	Author chain E； PDBConstruct 2–31； UniProt 590–619 Author chain F； PDBConstruct 2–31； UniProt 590–619 Author chain H； PDBConstruct 2–31； UniProt 590–619 Author chain I； PDBConstruct 2–31； UniProt 590–619

Mitochondrial import receptor subunit TOM20 homolog

Homo sapiens

UniProt Q15388

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein heterocomplex	Heteromer	Protein 3	Protein fem-1 homolog B × 1 （Q9Z2G0） Folliculin-interacting protein 1 × 1 （Q68FD7） ZINC ION × 2 4-(2-HYDROXYETHYL)-1-PIPERAZINE ETHANESULFONIC ACID × 1 PHOSPHATE ION × 1	Consistent with protein count
3	Protein heterocomplex	Heteromer	Protein 3	Protein fem-1 homolog B × 1 （Q9Z2G0） Folliculin-interacting protein 1 × 1 （Q68FD7） ZINC ION × 2 SULFATE ION × 1	Consistent with protein count
4	Protein heterocomplex	Heteromer	Protein 3	Protein fem-1 homolog B × 1 （Q9Z2G0） Folliculin-interacting protein 1 × 1 （Q68FD7） ZINC ION × 2	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	TOM20_HUMAN
Isoform	—
PDB entities	3
Chains and sequence ranges	Author chain J； PDBConstruct 1–66； UniProt 62–127 Author chain K； PDBConstruct 1–66； UniProt 62–127 Author chain M； PDBConstruct 1–66； UniProt 62–127

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

▼

2. Structure Basics 2. Structure Basics

Entry ID entry_id	8uy3
Deposition date deposition_date	2023-11-12
Last revision last_revision	2025-03-19
Structure title title	Fem1B with FNIP1 and Tom20 fragment
Keywords keywords	Fem1B FNP1 ubiquitin, proteasome, reductive stress response, TOM complex electron transport chain mitochondria, PROTEIN BINDING; PROTEIN BINDING
Experimental Method method	X-RAY DIFFRACTION

▼

3. Official assembly/model SAXS Official SAXS Profiles

This page reads only the latest assembly calculations. Every curve maps to an explicit PDB entry, official biological assembly and coordinate model.

8uy3__assembly_1__model_1

Assembly 1 · Model 1 · CRYSOL 4.1.3-1-20251215 (887e7ef)

Download this curve (.dat)

8uy3__assembly_1__model_1 | I(q)

100 plotted points; both axes use logarithmic scales.

8uy3__assembly_1__model_1 | P(r) · Pending

P(r) will be calculated and displayed separately for the same assembly/model.

Rg（Guinier）	30.64 Å
Rg (electron density)	30.98 Å
Total Rg	31.27 Å
Atom count	3617
Residues	460
Excluded volume	64279 Å³
Maximum q	0.500 Å⁻¹

Assembly	Model	Structure unit	Oligomeric description	Status	CRYSOL	Actions
1	1	`8uy3__assembly_1__model_1`	trimeric （3）	Success	4.1.3-1-20251215 (887e7ef)	View Download
2	1	`8uy3__assembly_2__model_1`	dimeric （2）	Success	4.1.3-1-20251215 (887e7ef)	View Download
3	1	`8uy3__assembly_3__model_1`	trimeric （3）	Success	4.1.3-1-20251215 (887e7ef)	View Download
4	1	`8uy3__assembly_4__model_1`	trimeric （3）	Success	4.1.3-1-20251215 (887e7ef)	View Download

▶

4. Crystallography and Experiment 4. Crystallography & Experiment

▶

5. Entities and Polymers Entities & Polymers (7)

Entity 1 Entity 1 polymer

Description description	Protein fem-1 homolog B
Molecular weight formula_weight	42362.3 kDa
Source method src_method	man
Copy count entity_count	4

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	A,C,B,D
Atom count atom_count	13741

SGSSMEGLAGYVYKAASEGKVLTLAALLLNRSESDIRYLLGYVSQQGGQRSTPLIIAARNGHAKVVRLLLEHYRVQTQQTGTVRFDGYVIDGATALWCAAGAGHFEVVKLLVSHGANVNHTTVTNSTPLRAACFDGRLDIVKYLVENNANISIANKYDNTCLMIAAYKGHTDVVRYLLEQRADPNAKAHCGATALHFAAEAGHIDIVKELIKWRAAIVVNGHGMTPLKVAAESCKADVVELLLSHADCDRRSRIEALELLGASFANDRENYDIMKTYHYLYLAMLERFQDGDNILEKEVLPPIHAYGNRTECRNPQELEAIRQDRDALHMEGLIVRERILGADNIDVSHPIIYRGAVYADNMEFEQCIKLWLHALHLRQKG

Source Source

Source organism organism	Mus musculus
Common name common_name	house mouse
NCBI taxonomy ID ncbi_taxonomy_id	10090
Host organism host_organism	Escherichia coli

Entity 2 Entity 2 polymer

Description description	Folliculin-interacting protein 1
Molecular weight formula_weight	3505.0 kDa
Source method src_method	man
Copy count entity_count	4

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	E,F,H,I

GRNKSSLLFKESEETRTPNCNCKYCSHPVLG

Source Source

Source organism organism	Mus musculus
Common name common_name	house mouse
NCBI taxonomy ID ncbi_taxonomy_id	10090
Host organism host_organism	Escherichia coli

Entity 3 Entity 3 polymer

Description description	Mitochondrial import receptor subunit TOM20 homolog
Molecular weight formula_weight	7364.4 kDa
Source method src_method	man
Copy count entity_count	3

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	J,K,M

DAEAVQKFFLEEIQLGEELLAQGEYEKGVDHLTNAIAVCGQPQQLLQVLQQTLPPPVFQMLLTKLP

Source Source

Source organism organism	Homo sapiens
Common name common_name	human
NCBI taxonomy ID ncbi_taxonomy_id	9606
Host organism host_organism	Escherichia coli

Entity 4 Entity 4 non-polymer

Description description	ZINC ION
Molecular weight formula_weight	65.4 kDa
Source method src_method	syn
Copy count entity_count	8

Entity 5 Entity 5 non-polymer

Description description	4-(2-HYDROXYETHYL)-1-PIPERAZINE ETHANESULFONIC ACID
Molecular weight formula_weight	238.3 kDa
Source method src_method	syn
Copy count entity_count	1

Entity 6 Entity 6 non-polymer

Description description	PHOSPHATE ION
Molecular weight formula_weight	95.0 kDa
Source method src_method	syn
Copy count entity_count	1

Entity 7 Entity 7 non-polymer

Description description	SULFATE ION
Molecular weight formula_weight	96.1 kDa
Source method src_method	syn
Copy count entity_count	2

▶

Unit-cell length a length_a	183.715 Å
Unit-cell length b length_b	183.715 Å
Unit-cell length c length_c	183.715 Å
Unit-cell angle α angle_alpha	90.00 °
Unit-cell angle β angle_beta	90.00 °
Unit-cell angle γ angle_gamma	90.00 °
Molecules per unit cell Z z_pdb	48

Space group space_group	P 21 3
Space-group number int_tables_number	198
Crystal system crystal_system	cubic

Resolution resolution	3.200 Å
Low-resolution limit resolution_low	45.930 Å
R factor r_factor	0.2404
R_work r_work	0.2391
R_free r_free	0.2644

Fem1B with FNIP1 and Tom20 fragment

1. Protein Identity and Related Structures Protein Identity & Related Structures

Protein fem-1 homolog B

State in the Current Structure

Other States of the Same Protein in the Database

Folliculin-interacting protein 1

State in the Current Structure

Other States of the Same Protein in the Database

Mitochondrial import receptor subunit TOM20 homolog

State in the Current Structure

Other States of the Same Protein in the Database

2. Structure Basics 2. Structure Basics

3. Official assembly/model SAXS Official SAXS Profiles

8uy3__assembly_1__model_1

8uy3__assembly_1__model_1 | I(q)

8uy3__assembly_1__model_1 | P(r) · Pending

4. Crystallography and Experiment 4. Crystallography & Experiment

Unit cell Unit Cell

Symmetry Symmetry

Refinement Refinement

Diffraction experiment Diffraction

5. Entities and Polymers Entities & Polymers (7)

Entity 1 Entity 1 polymer

Polymer Polymer

Source Source

Entity 2 Entity 2 polymer

Polymer Polymer

Source Source

Entity 3 Entity 3 polymer

Polymer Polymer

Source Source

Entity 4 Entity 4 non-polymer

Entity 5 Entity 5 non-polymer

Entity 6 Entity 6 non-polymer

Entity 7 Entity 7 non-polymer

7. Citations (1)

Fem1B with FNIP1 and Tom20 fragment

1. Protein Identity and Related Structures Protein Identity & Related Structures

Protein fem-1 homolog B

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

Folliculin-interacting protein 1

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

Mitochondrial import receptor subunit TOM20 homolog

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

2. Structure Basics 2. Structure Basics

3. Official assembly/model SAXS Official SAXS Profiles

8uy3__assembly_1__model_1

8uy3__assembly_1__model_1 | I(q)

8uy3__assembly_1__model_1 | P(r) · Pending

4. Crystallography and Experiment 4. Crystallography & Experiment

Unit cell Unit Cell

Symmetry Symmetry

Refinement Refinement

Diffraction experiment Diffraction

5. Entities and Polymers Entities & Polymers (7)

Entity 1 Entity 1 polymer

Polymer Polymer

Source Source

Entity 2 Entity 2 polymer

Polymer Polymer

Source Source

Entity 3 Entity 3 polymer

Polymer Polymer

Source Source

Entity 4 Entity 4 non-polymer

Entity 5 Entity 5 non-polymer

Entity 6 Entity 6 non-polymer

Entity 7 Entity 7 non-polymer

7. Citations (1)