9euf

Cryo-EM structure of Staphylococcus aureus bacteriophage phi812 baseplate in the pre-contraction state - complete

Method: ELECTRON MICROSCOPY

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1. Protein Identity and Related Structures Protein Identity & Related Structures

DUF4815 domain-containing protein

OrganismNot specified

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein heterocomplex	Heteromer	Protein 189	Receptor binding protein × 18 （A0A0U1WIJ9） CBM-cenC domain-containing protein × 18 （A0A0U1UXW5） NlpC/P60 domain-containing protein × 3 （A0A0U1UXW0） GP-PDE domain-containing protein × 3 （A0A0U1WFD7） Baseplate component × 12 （A0A0U1WF63） Peptidase C51 domain-containing protein × 3 （A0A0U1X189） Baseplate component × 6 （A0A0U1UXD6） TmpF × 6 （A0A0U1WGD3） Baseplate wedge subunit × 6 （A0A0U1UXD7） Putative baseplate component × 6 （A0A0U1X2L4） Major tail sheath protein × 18 （A0A0U1WZ79） Capsid protein × 18 （A1YTP2） Baseplate protein × 36 （A0A0U1WGQ5）	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	A0A8E5NSA0_9CAUD
Isoform	—
PDB entities	1
Chains and sequence ranges	Author chain 0； PDBConstruct 1–1152； UniProt 1–1152 Author chain 1； PDBConstruct 1–1152； UniProt 1–1152 Author chain 2； PDBConstruct 1–1152； UniProt 1–1152 Author chain 3； PDBConstruct 1–1152； UniProt 1–1152 Author chain 4； PDBConstruct 1–1152； UniProt 1–1152 Author chain h； PDBConstruct 1–1152； UniProt 1–1152 Author chain i； PDBConstruct 1–1152； UniProt 1–1152 Author chain j； PDBConstruct 1–1152； UniProt 1–1152 Author chain k； PDBConstruct 1–1152； UniProt 1–1152 Author chain l； PDBConstruct 1–1152； UniProt 1–1152 Author chain m； PDBConstruct 1–1152； UniProt 1–1152 Author chain z； PDBConstruct 1–1152； UniProt 1–1152

Receptor binding protein

OrganismNot specified

UniProt A0A0U1WIJ9

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein heterocomplex	Heteromer	Protein 189	DUF4815 domain-containing protein × 36 （A0A8E5NSA0） CBM-cenC domain-containing protein × 18 （A0A0U1UXW5） NlpC/P60 domain-containing protein × 3 （A0A0U1UXW0） GP-PDE domain-containing protein × 3 （A0A0U1WFD7） Baseplate component × 12 （A0A0U1WF63） Peptidase C51 domain-containing protein × 3 （A0A0U1X189） Baseplate component × 6 （A0A0U1UXD6） TmpF × 6 （A0A0U1WGD3） Baseplate wedge subunit × 6 （A0A0U1UXD7） Putative baseplate component × 6 （A0A0U1X2L4） Major tail sheath protein × 18 （A0A0U1WZ79） Capsid protein × 18 （A1YTP2） Baseplate protein × 36 （A0A0U1WGQ5）	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	A0A0U1WIJ9_9CAUD
Isoform	—
PDB entities	2
Chains and sequence ranges	Author chain 5； PDBConstruct 1–458； UniProt 1–458 Author chain 6； PDBConstruct 1–458； UniProt 1–458 Author chain 7； PDBConstruct 1–458； UniProt 1–458 Author chain n； PDBConstruct 1–458； UniProt 1–458 Author chain o； PDBConstruct 1–458； UniProt 1–458 Author chain p； PDBConstruct 1–458； UniProt 1–458

CBM-cenC domain-containing protein

OrganismNot specified

UniProt A0A0U1UXW5

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein heterocomplex	Heteromer	Protein 189	DUF4815 domain-containing protein × 36 （A0A8E5NSA0） Receptor binding protein × 18 （A0A0U1WIJ9） NlpC/P60 domain-containing protein × 3 （A0A0U1UXW0） GP-PDE domain-containing protein × 3 （A0A0U1WFD7） Baseplate component × 12 （A0A0U1WF63） Peptidase C51 domain-containing protein × 3 （A0A0U1X189） Baseplate component × 6 （A0A0U1UXD6） TmpF × 6 （A0A0U1WGD3） Baseplate wedge subunit × 6 （A0A0U1UXD7） Putative baseplate component × 6 （A0A0U1X2L4） Major tail sheath protein × 18 （A0A0U1WZ79） Capsid protein × 18 （A1YTP2） Baseplate protein × 36 （A0A0U1WGQ5）	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	A0A0U1UXW5_9CAUD
Isoform	—
PDB entities	3
Chains and sequence ranges	Author chain 8； PDBConstruct 1–640； UniProt 1–640 Author chain 9； PDBConstruct 1–640； UniProt 1–640 Author chain AA； PDBConstruct 1–640； UniProt 1–640 Author chain q； PDBConstruct 1–640； UniProt 1–640 Author chain r； PDBConstruct 1–640； UniProt 1–640 Author chain s； PDBConstruct 1–640； UniProt 1–640

NlpC/P60 domain-containing protein

OrganismNot specified

UniProt A0A0U1UXW0

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein heterocomplex	Heteromer	Protein 189	DUF4815 domain-containing protein × 36 （A0A8E5NSA0） Receptor binding protein × 18 （A0A0U1WIJ9） CBM-cenC domain-containing protein × 18 （A0A0U1UXW5） GP-PDE domain-containing protein × 3 （A0A0U1WFD7） Baseplate component × 12 （A0A0U1WF63） Peptidase C51 domain-containing protein × 3 （A0A0U1X189） Baseplate component × 6 （A0A0U1UXD6） TmpF × 6 （A0A0U1WGD3） Baseplate wedge subunit × 6 （A0A0U1UXD7） Putative baseplate component × 6 （A0A0U1X2L4） Major tail sheath protein × 18 （A0A0U1WZ79） Capsid protein × 18 （A1YTP2） Baseplate protein × 36 （A0A0U1WGQ5）	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	A0A0U1UXW0_9CAUD
Isoform	—
PDB entities	4
Chains and sequence ranges	Author chain A； PDBConstruct 1–295； UniProt 1–295

GP-PDE domain-containing protein

OrganismNot specified

UniProt A0A0U1WFD7

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein heterocomplex	Heteromer	Protein 189	DUF4815 domain-containing protein × 36 （A0A8E5NSA0） Receptor binding protein × 18 （A0A0U1WIJ9） CBM-cenC domain-containing protein × 18 （A0A0U1UXW5） NlpC/P60 domain-containing protein × 3 （A0A0U1UXW0） Baseplate component × 12 （A0A0U1WF63） Peptidase C51 domain-containing protein × 3 （A0A0U1X189） Baseplate component × 6 （A0A0U1UXD6） TmpF × 6 （A0A0U1WGD3） Baseplate wedge subunit × 6 （A0A0U1UXD7） Putative baseplate component × 6 （A0A0U1X2L4） Major tail sheath protein × 18 （A0A0U1WZ79） Capsid protein × 18 （A1YTP2） Baseplate protein × 36 （A0A0U1WGQ5）	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	A0A0U1WFD7_9CAUD
Isoform	—
PDB entities	5
Chains and sequence ranges	Author chain B； PDBConstruct 1–848； UniProt 1–848

Baseplate component

OrganismNot specified

UniProt A0A0U1WF63

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein heterocomplex	Heteromer	Protein 189	DUF4815 domain-containing protein × 36 （A0A8E5NSA0） Receptor binding protein × 18 （A0A0U1WIJ9） CBM-cenC domain-containing protein × 18 （A0A0U1UXW5） NlpC/P60 domain-containing protein × 3 （A0A0U1UXW0） GP-PDE domain-containing protein × 3 （A0A0U1WFD7） Peptidase C51 domain-containing protein × 3 （A0A0U1X189） Baseplate component × 6 （A0A0U1UXD6） TmpF × 6 （A0A0U1WGD3） Baseplate wedge subunit × 6 （A0A0U1UXD7） Putative baseplate component × 6 （A0A0U1X2L4） Major tail sheath protein × 18 （A0A0U1WZ79） Capsid protein × 18 （A1YTP2） Baseplate protein × 36 （A0A0U1WGQ5）	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	A0A0U1WF63_9CAUD
Isoform	—
PDB entities	6
Chains and sequence ranges	Author chain C； PDBConstruct 1–348； UniProt 1–348 Author chain D； PDBConstruct 1–348； UniProt 1–348 Author chain I； PDBConstruct 1–348； UniProt 1–348 Author chain K； PDBConstruct 1–348； UniProt 1–348

Peptidase C51 domain-containing protein

OrganismNot specified

UniProt A0A0U1X189

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein heterocomplex	Heteromer	Protein 189	DUF4815 domain-containing protein × 36 （A0A8E5NSA0） Receptor binding protein × 18 （A0A0U1WIJ9） CBM-cenC domain-containing protein × 18 （A0A0U1UXW5） NlpC/P60 domain-containing protein × 3 （A0A0U1UXW0） GP-PDE domain-containing protein × 3 （A0A0U1WFD7） Baseplate component × 12 （A0A0U1WF63） Baseplate component × 6 （A0A0U1UXD6） TmpF × 6 （A0A0U1WGD3） Baseplate wedge subunit × 6 （A0A0U1UXD7） Putative baseplate component × 6 （A0A0U1X2L4） Major tail sheath protein × 18 （A0A0U1WZ79） Capsid protein × 18 （A1YTP2） Baseplate protein × 36 （A0A0U1WGQ5）	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	A0A0U1X189_9CAUD
Isoform	—
PDB entities	7
Chains and sequence ranges	Author chain E； PDBConstruct 1–808； UniProt 1–808

Baseplate component

OrganismNot specified

UniProt A0A0U1UXD6

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein heterocomplex	Heteromer	Protein 189	DUF4815 domain-containing protein × 36 （A0A8E5NSA0） Receptor binding protein × 18 （A0A0U1WIJ9） CBM-cenC domain-containing protein × 18 （A0A0U1UXW5） NlpC/P60 domain-containing protein × 3 （A0A0U1UXW0） GP-PDE domain-containing protein × 3 （A0A0U1WFD7） Baseplate component × 12 （A0A0U1WF63） Peptidase C51 domain-containing protein × 3 （A0A0U1X189） TmpF × 6 （A0A0U1WGD3） Baseplate wedge subunit × 6 （A0A0U1UXD7） Putative baseplate component × 6 （A0A0U1X2L4） Major tail sheath protein × 18 （A0A0U1WZ79） Capsid protein × 18 （A1YTP2） Baseplate protein × 36 （A0A0U1WGQ5）	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	A0A0U1UXD6_9CAUD
Isoform	—
PDB entities	8
Chains and sequence ranges	Author chain F； PDBConstruct 1–174； UniProt 1–174 Author chain G； PDBConstruct 1–174； UniProt 1–174

TmpF

OrganismNot specified

UniProt A0A0U1WGD3

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein heterocomplex	Heteromer	Protein 189	DUF4815 domain-containing protein × 36 （A0A8E5NSA0） Receptor binding protein × 18 （A0A0U1WIJ9） CBM-cenC domain-containing protein × 18 （A0A0U1UXW5） NlpC/P60 domain-containing protein × 3 （A0A0U1UXW0） GP-PDE domain-containing protein × 3 （A0A0U1WFD7） Baseplate component × 12 （A0A0U1WF63） Peptidase C51 domain-containing protein × 3 （A0A0U1X189） Baseplate component × 6 （A0A0U1UXD6） Baseplate wedge subunit × 6 （A0A0U1UXD7） Putative baseplate component × 6 （A0A0U1X2L4） Major tail sheath protein × 18 （A0A0U1WZ79） Capsid protein × 18 （A1YTP2） Baseplate protein × 36 （A0A0U1WGQ5）	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	A0A0U1WGD3_9CAUD
Isoform	—
PDB entities	9
Chains and sequence ranges	Author chain H； PDBConstruct 1–1019； UniProt 1–1019 Author chain J； PDBConstruct 1–1019； UniProt 1–1019

Baseplate wedge subunit

OrganismNot specified

UniProt A0A0U1UXD7

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein heterocomplex	Heteromer	Protein 189	DUF4815 domain-containing protein × 36 （A0A8E5NSA0） Receptor binding protein × 18 （A0A0U1WIJ9） CBM-cenC domain-containing protein × 18 （A0A0U1UXW5） NlpC/P60 domain-containing protein × 3 （A0A0U1UXW0） GP-PDE domain-containing protein × 3 （A0A0U1WFD7） Baseplate component × 12 （A0A0U1WF63） Peptidase C51 domain-containing protein × 3 （A0A0U1X189） Baseplate component × 6 （A0A0U1UXD6） TmpF × 6 （A0A0U1WGD3） Putative baseplate component × 6 （A0A0U1X2L4） Major tail sheath protein × 18 （A0A0U1WZ79） Capsid protein × 18 （A1YTP2） Baseplate protein × 36 （A0A0U1WGQ5）	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	A0A0U1UXD7_9CAUD
Isoform	—
PDB entities	10
Chains and sequence ranges	Author chain L； PDBConstruct 1–234； UniProt 1–234 Author chain O； PDBConstruct 1–234； UniProt 1–234

Putative baseplate component

OrganismNot specified

UniProt A0A0U1X2L4

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein heterocomplex	Heteromer	Protein 189	DUF4815 domain-containing protein × 36 （A0A8E5NSA0） Receptor binding protein × 18 （A0A0U1WIJ9） CBM-cenC domain-containing protein × 18 （A0A0U1UXW5） NlpC/P60 domain-containing protein × 3 （A0A0U1UXW0） GP-PDE domain-containing protein × 3 （A0A0U1WFD7） Baseplate component × 12 （A0A0U1WF63） Peptidase C51 domain-containing protein × 3 （A0A0U1X189） Baseplate component × 6 （A0A0U1UXD6） TmpF × 6 （A0A0U1WGD3） Baseplate wedge subunit × 6 （A0A0U1UXD7） Major tail sheath protein × 18 （A0A0U1WZ79） Capsid protein × 18 （A1YTP2） Baseplate protein × 36 （A0A0U1WGQ5）	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	A0A0U1X2L4_9CAUD
Isoform	—
PDB entities	11
Chains and sequence ranges	Author chain M； PDBConstruct 1–263； UniProt 1–263 Author chain N； PDBConstruct 1–263； UniProt 1–263

Major tail sheath protein

OrganismNot specified

UniProt A0A0U1WZ79

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein heterocomplex	Heteromer	Protein 189	DUF4815 domain-containing protein × 36 （A0A8E5NSA0） Receptor binding protein × 18 （A0A0U1WIJ9） CBM-cenC domain-containing protein × 18 （A0A0U1UXW5） NlpC/P60 domain-containing protein × 3 （A0A0U1UXW0） GP-PDE domain-containing protein × 3 （A0A0U1WFD7） Baseplate component × 12 （A0A0U1WF63） Peptidase C51 domain-containing protein × 3 （A0A0U1X189） Baseplate component × 6 （A0A0U1UXD6） TmpF × 6 （A0A0U1WGD3） Baseplate wedge subunit × 6 （A0A0U1UXD7） Putative baseplate component × 6 （A0A0U1X2L4） Capsid protein × 18 （A1YTP2） Baseplate protein × 36 （A0A0U1WGQ5）	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	A0A0U1WZ79_9CAUD
Isoform	—
PDB entities	12
Chains and sequence ranges	Author chain P； PDBConstruct 1–587； UniProt 1–587 Author chain Q； PDBConstruct 1–587； UniProt 1–587 Author chain T； PDBConstruct 1–587； UniProt 1–587 Author chain U； PDBConstruct 1–587； UniProt 1–587 Author chain X； PDBConstruct 1–587； UniProt 1–587 Author chain Y； PDBConstruct 1–587； UniProt 1–587

Capsid protein

OrganismNot specified

UniProt A1YTP2

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein heterocomplex	Heteromer	Protein 189	DUF4815 domain-containing protein × 36 （A0A8E5NSA0） Receptor binding protein × 18 （A0A0U1WIJ9） CBM-cenC domain-containing protein × 18 （A0A0U1UXW5） NlpC/P60 domain-containing protein × 3 （A0A0U1UXW0） GP-PDE domain-containing protein × 3 （A0A0U1WFD7） Baseplate component × 12 （A0A0U1WF63） Peptidase C51 domain-containing protein × 3 （A0A0U1X189） Baseplate component × 6 （A0A0U1UXD6） TmpF × 6 （A0A0U1WGD3） Baseplate wedge subunit × 6 （A0A0U1UXD7） Putative baseplate component × 6 （A0A0U1X2L4） Major tail sheath protein × 18 （A0A0U1WZ79） Baseplate protein × 36 （A0A0U1WGQ5）	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	A1YTP2_9CAUD
Isoform	—
PDB entities	13
Chains and sequence ranges	Author chain R； PDBConstruct 1–142； UniProt 1–142 Author chain S； PDBConstruct 1–142； UniProt 1–142 Author chain V； PDBConstruct 1–142； UniProt 1–142 Author chain W； PDBConstruct 1–142； UniProt 1–142 Author chain Z； PDBConstruct 1–142； UniProt 1–142 Author chain a； PDBConstruct 1–142； UniProt 1–142

Baseplate protein

Staphylococcus phage 812

UniProt A0A0U1WGQ5

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein heterocomplex	Heteromer	Protein 189	DUF4815 domain-containing protein × 36 （A0A8E5NSA0） Receptor binding protein × 18 （A0A0U1WIJ9） CBM-cenC domain-containing protein × 18 （A0A0U1UXW5） NlpC/P60 domain-containing protein × 3 （A0A0U1UXW0） GP-PDE domain-containing protein × 3 （A0A0U1WFD7） Baseplate component × 12 （A0A0U1WF63） Peptidase C51 domain-containing protein × 3 （A0A0U1X189） Baseplate component × 6 （A0A0U1UXD6） TmpF × 6 （A0A0U1WGD3） Baseplate wedge subunit × 6 （A0A0U1UXD7） Putative baseplate component × 6 （A0A0U1X2L4） Major tail sheath protein × 18 （A0A0U1WZ79） Capsid protein × 18 （A1YTP2）	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	A0A0U1WGQ5_9CAUD
Isoform	—
PDB entities	14
Chains and sequence ranges	Author chain b； PDBConstruct 1–173； UniProt 1–173 Author chain c； PDBConstruct 1–173； UniProt 1–173 Author chain d； PDBConstruct 1–173； UniProt 1–173 Author chain e； PDBConstruct 1–173； UniProt 1–173 Author chain f； PDBConstruct 1–173； UniProt 1–173 Author chain g； PDBConstruct 1–173； UniProt 1–173 Author chain t； PDBConstruct 1–173； UniProt 1–173 Author chain u； PDBConstruct 1–173； UniProt 1–173 Author chain v； PDBConstruct 1–173； UniProt 1–173 Author chain w； PDBConstruct 1–173； UniProt 1–173 Author chain x； PDBConstruct 1–173； UniProt 1–173 Author chain y； PDBConstruct 1–173； UniProt 1–173

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

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2. Structure Basics 2. Structure Basics

Entry ID entry_id	9euf
Deposition date deposition_date	2024-03-27
Structure title title	Cryo-EM structure of Staphylococcus aureus bacteriophage phi812 baseplate in the pre-contraction state - complete
Keywords keywords	bacteriophage, phage, contractile, phi812, baseplate, VIRUS; VIRUS
Experimental Method method	ELECTRON MICROSCOPY

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3. Official assembly/model SAXS Official SAXS Profiles

This page reads only the latest assembly calculations. Every curve maps to an explicit PDB entry, official biological assembly and coordinate model.

9euf__assembly_1__model_1

Assembly 1 · Model 1 · CRYSOL 4.1.3-1-20251215 (887e7ef)

Download this curve (.dat)

9euf__assembly_1__model_1 | I(q)

100 plotted points; both axes use logarithmic scales.

9euf__assembly_1__model_1 | P(r) · Pending

P(r) will be calculated and displayed separately for the same assembly/model.

Rg（Guinier）	0.00 Å
Rg (electron density)	161.10 Å
Total Rg	159.90 Å
Atom count	672876
Residues	90690
Excluded volume	7592900 Å³
Maximum q	0.500 Å⁻¹

Assembly	Model	Structure unit	Oligomeric description	Status	CRYSOL	Actions
1	1	`9euf__assembly_1__model_1`	189-meric （189）	Success	4.1.3-1-20251215 (887e7ef)	View Download

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4. Crystallography and Experiment 4. Crystallography & Experiment

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5. Entities and Polymers Entities & Polymers (14)

Entity 1 Entity 1 polymer

Description description	DUF4815 domain-containing protein
Molecular weight formula_weight	129263.0 kDa
Source method src_method	nat
Copy count entity_count	12

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	0,1,2,3,4,h,i,j,k,l,m,z
Atom count atom_count	224293

MAINFKGSPYLDRFDPSKDRTKVLFNPDRPLQQAELNEMQSIDQYYLKNLGDAIFKDGDKQSGLGFTLSEDNVLTVNPGYVYINGKIRYYDNDDSVKITGVGKETIGIKLTERIVTPDEDASLLDQTSGVPSYFSKGADRLEEKMSLTVNDPTSATIYTFMDGDLYIQSTNAEMDKINKVLAERTYDESGSYKVNGFELFSEGNAEDDDHVSVVVDAGKAYVKGFKVDKPVSTRISVPKSYDLGTAENESTIFNKSNNSISLANSPVKEIRRVTGQVLIEKERVTRGAQGDGQDFLSNNTAFEIVKVWTETSPGVTTKEYKQGEDFRLTDGQTIDWSPQGQEPSGGTSYYVSYKYNKRMEAGKDYEVTTQGEGLSKKWYINFTPSNGAKPIDQTVVLVDYTYYLARKDSVFINKYGDIAILPGEPNIMRLVTPPLNTDPENLQLGTVTVLPDSDEAVCISFAITRLSMEDLQKVKTRVDNLEYNQAVNALDDGAMEGQNPLTLRSVFSEGFISLDKADITHPDFGIVFSFEDAEATLAYTEAVNQPKIIPGDTTAHIWGRLISAPFTEERTIYQGQASETLNVNPYNIPNKQGVLKLTPSEDNWIDTENVTITEQKTKKVTMKRFWRHNESYYGETEHYLYSNLQLDAGQKWKGETYAYDREHGRTGTLLESGGQRTLEEMIEFIRIRDVSFEVKGLNPNDNNLYLLFDGVRCAITPATGYRKGSEDGTIMTDAKGTAKGKFTIPAGIRCGNREVTLKNANSTSATTYTAQGRKKTAQDIIIRTRVTVNLVDPLAQSFQYDENRTISSLGLYFASKGDKQSNVVIQIRGMGDQGYPNKTIYAETVMNADDIKVSNNASAETRVYFDDPMMAEGGKEYAIVIITENSDYTMWVGTRTKPKIDKPNEVISGNPYLQGVLFSSSNASTWTPHQNSDLKFGIYTSKFNETATIEFEPIKDVSADRIVLMSTYLTPERTGCTWEMKLILDDMASSTTFDQLKWEPIGNYQDLDVLGLARQVKLRATFESNRYISPLMSSSDLTFTTFLTELTGSYVGRAIDMTEAPYNTVRFSYEAFLPKGTKVVPKYSADDGKTWKTFTKSPTTTRANNEFTRYVIDEKVKSSGTNTKLQVRLDLSTENSFLRPRVRRLMVTTRDE

Entity 2 Entity 2 polymer

Description description	Receptor binding protein
Molecular weight formula_weight	50474.1 kDa
Source method src_method	nat
Copy count entity_count	6

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	5,6,7,n,o,p

MALNFTTITENNVIRDLTTQVNNIGEELTKERNIFDITDDLVYNFNKSQKIKLTDDKGLTKSYGNITALRDIKEPGYYYIGARTLATLLDRPDMESLDVVLHVVPLDTSSKVVQHLYTLSTNNNQIKMLYRFVSGNSSSEWQFIQGLPSNKNAVISGTNILDIASPGVYFVMGMTGGMPSGVSSGFLDLSVDANDNRLARLTDAETGKEYTSIKKPTGTYTAWKKEFEPKDMEKYLLSSIRDDGSASFPLLVYTSDSKTFQQAIIDHIDRTGQTTFTFYVQGGVSGSPMSNSCRGLFMSDTPNTSSLHGVYNAIGTDGRNVTGSVVGSNWTSPKTSPSHKELWTGAQSFLSTGTTKNLSDDISNYSYVEVYTTHKTTEKTKGNDNTGTICHKFYLDGSGTYVCSGTFVSGDRTDTKPPITEFYRVGVSFKGSTWTLVDSAVQNSKTQYVTRIIGINMP

Entity 3 Entity 3 polymer

Description description	CBM-cenC domain-containing protein
Molecular weight formula_weight	72654.7 kDa
Source method src_method	nat
Copy count entity_count	6

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	8,9,AA,q,r,s

MAFNYTPLTETQKLKDMYPKVNDIGNFLKTEVNLSDVKQISQPDFNNILASIPDSGNYYVTNSKGAPSGEATAGFVRLDKRNVNYYKIYYSPYSSNKMYIKTYANGTVYDWISFKLDEGSLYNEGNTLNVKELTESTTQYATLVNPPKENLNTGWVNYKESKNGVSSLVEFNPVNSTSTFKMIRKLPVQEQKPNLLKDSLFVYPETSYSNIKTDNWDTPPFWGYSSNSGRSGVRFRGENTVQIDDGSDTYPSVVSNRFKMGKELSVGDTVTVSVYAKINDPALLKDNLVYFELAGYDTVDDTSKNPYTGGRREITASEITTEWKKYSFTFTIPENTIGASGVKVNYVSLLLRMNCSSSKGNGAVVYYALPKLEKSSKVTPFITHENDVRKYDEIWSNWQEVISKDELKGHSPVDIEYNDYFKYQWWKSEVNEKSLKDLAMTVPQGYHTFYCQGSIAGTPKGRSIRGTIQVDYDKCDPYRANKFVKLLFTDTEGIPYTLYYGGYNQGWKPLKQSETSTLLWKGTLDFGSTEAVNLNDSLDNYDLIEVTYWTRSAGHFSTKRLDIKNTSNLLYIRDFNISNDSTGSSVDFFEGYCTFPTRTSVQPGMVKSITLDGSTNTTKVASWNEKERIKVYNIMGINRG

Entity 4 Entity 4 polymer

Description description	NlpC/P60 domain-containing protein
Molecular weight formula_weight	34645.9 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	A

MATDKEAKDVIDKFIDNVFNFDVLTKERIKEKDEEIKKITTDDMYEKVVYIRPYVGVIQSLNPQHVQYESFSNNGYDIEAELSFRKVSYLVDKGSIPTDSLSTLTVHLVERNQELLIDYFDEIQDVLYGEYMEEEYVFDEDVPLSTILALDLNDNLKSLSNIKYMFKGAPKENPFGTDKDVYIDTYNLLYWLYLGEDEELAYPMNINYFFTEGRFFTIFGKGHKYKVDVSKFIVGDILFFGRSDTNIGIYVGDGEFISMMGKFPKDETPIGKYKLDDYWNEFNGRVMRFDEEVYI

Entity 5 Entity 5 polymer

Description description	GP-PDE domain-containing protein
Molecular weight formula_weight	96199.5 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	B

MVVRFQSSMGRSLKRVDSDDLNVKGLVLATVSKINYKYQSVEVKVNNLTLGSRIGDDGSLAVPYPKSFIGRTPEGSVFGTKPLITEGSVVLIGFLNDDINSPIILSVYGDNEQNKMINTNPLDGGKFDTESVYKYSSSLYEILPSLNYKYDDGEGTSIRTYNGKSFFSMTSGEEEKPQATDFYTGTEYQDLFTSYYGNKTLIEPRIQKAPNMLFKHQGVFYDDGTPDNHITTLFISERGDIRASVLNTETQKRTTQEMSSDGSYRVIKQDDDLMLDEAQVWIEYGISEDNKFYIKNDKHKFEFTDEGIYIDDKPMLENLDESIAEAMKNLNEIQKELDDINYLLKGVGKDNLEELIESTKESIEASKKATSDVNRLTTQIAEVSGRTEGIITQFQKFRDETFKDFYEDASTVINEVNQNFPTMKTDVKTLKTKVDNLEKTEIPNIKTRLTELENNNNNADKIISDRGEHIGAMIQLEENVTVPMRKYMPIPWSKVTYNNAEFWDSNNPTRLVVPKGITKVRVAGNVLWDSNATGQRMLRILKNGTYSIGLPYTRDVAISTAPQNGTSGVIPVKEGDYFEFEAFQDSEGDRQFRADPYTWFSIEAIELETETMEKDFMLIGHRGATGYTDEHTIKGYQMALDKGADYIELDLQLTKDNKLLCMHDSTIDRTTTGTGKVGDMTLSYIQTNFTSLNGEPIPSLDDVLNHFGTKVKYYIETKRPFDANMDRELLTQLKAKGLIGIGSERFQVIIQSFARESLINIHNQFSNIPLAYLTSTFSESEMDDCLSYGFYAIAPKYTTITKELVDLAHSKGLKVHAWTVNTKEEMQSLIQMGVDGFFTNYLDEYKKI

Entity 6 Entity 6 polymer

Description description	Baseplate component
Molecular weight formula_weight	39248.9 kDa
Source method src_method	nat
Copy count entity_count	4

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	C,D,I,K

MKTRKLTNILSKLIDKTMAGTSKITDFTPGSASRSLLEAVSLEIEQFYILTKENIDWGIQEGIIEAFDFQKRQSKRAYGDVTIQFYQPLDMRMYIPAGTTFTSTRQEYPQQFETLVDYYAEPDSTEIVVEVYCKETGVAGNVPEGTINTIASGSSLIRSVNNEYSFNTGTKEESQEDFKRRFHSFVESRGRATNKSVRYGALQIPDVEGVYVYEETGHITVFAHDRNGNLSDTLKEDIIDALQDYRPSGIMLDVTGVEKEEVNVSATVTISNKSRIGDTLQKHIESVIRSYLNNLKTSDDLIITDLIQAIMNIDDVLIYDVSFDNLDENIIVPPQGIIRAGEIKVELK

Entity 7 Entity 7 polymer

Description description	Peptidase C51 domain-containing protein
Molecular weight formula_weight	91364.3 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	E

MRRIRRPKVRIEIVTDDNTFTLRFEDTRDYNGDEFGAKLLGFQTKNSMEDDSSVFQINMAGDTYWDKLVMANDIIRIFITPNDDPNDKEGKQERLIQVGMVSQVSKVGSYGNDQTQFRITGQSFVKPFMKFGLGVIQEVQAVLPEVGWLIDGDGDNEVKFTGSSAHEVMTGIIRRFIPYMKYNYTEKTYNTIDNYLDYDDLSSWDEFEKLTEVSAFTNFDGSLKQLMDMVTARPFNELFFKNSEKTPGKAQLVLRKTPFNPTEWRALDMIKVPTEDFIEEDVGKSDVETYSIFTATPAGMLKELNGDVFSKPQFHPELTDRYGYTKFEVENIYLSTKSGSATEDSDSSGDDNGTERGTYSKIMKDLSNYGRDNISKGIDKYTSKLSSKYKNLKKAQAKKIIEKFVKEGKVTEKEYEKITGNKVDDELTSDNRPKLTKDKLKSILKEKFKTQDDFNNSKKKKKAKTDALKELTTKYRFGNKTHATTLLDEYIKYKGEPPNDEAFDKYLKAIEGVSNVATDTGSDASDSPLVMFSRMLFNWYHGNPNFYAGDIIVLGDPKYDLGKRLFIEDKQRGDTWEFYIESVEHKFDYKQGYYTTVGVTRGLKDAILEDGKGSPHRFAGLWNQSSDFMGGLMGEDTSKELKEKGVAEKQSSGDKDGGSDSGGAQDGGSLDSLKKYNGKLPKHDPSFVQPGNRHYKYQCTWYAYNRRGQLGIPVPLWGDAADWIGGAKGAGYGVGRTPKQGACVIWQRGVQGGSPQYGHVAFVEKVLDGGKKIFISEHNYATPNGYGTRTIDMSSAIGKNAQFIYDKK

Entity 8 Entity 8 polymer

Description description	Baseplate component
Molecular weight formula_weight	19998.7 kDa
Source method src_method	nat
Copy count entity_count	2

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	F,G

MNNFIPQPQGLLRFLNTLDTDLTSSHMNLLDEEVSFVSKFYTPQLQLSELAKKVLTNIKTDDIPVLEREFNDNTIIHKANDTLLKVQAPRMYMILQSIVLEAYAIVNCFVENPSSLKYLTEEDVSITRENLNYVADYLGNYDDYNSVVLDLRDLDLCFSAIELQLPLIKKEANV

Entity 9 Entity 9 polymer

Description description	TmpF
Molecular weight formula_weight	116389.9 kDa
Source method src_method	nat
Copy count entity_count	2

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	H,J

MANFLKNLHPLLRRDRNKKDNQDPNFALIDALNEEMNQVEKDAIESKLQSSLKTSTSEYLDKFGDWFGVYRKTDEKDDVYRARIIKYLLLKRGTNNAIIDAIKDYLGRDDIDVSVYEPFTNIFYTNKSHLNGEDHLMGYYYRFAVINVSIGDYFPVEIIDVINEFKPAGVTLYVTYDGASTIRGGAIIKWLDGLPKIETYQEFDRFTGYDDTFYGHINMNQSKDTDNSSSDIFKTNHSLINSLDVLTGSSSVGRQYINYGYVTSYVYNPGMTSSVNQISASTEGRGQEVPTDYYMYTSTKNNNTVELSMQTTSGVSYLYNNFNFRDYMSKYRPQVDLQSDEARRIVSDYIKELSIDYYLSAVIPPDESIEIKLQVYDFSINRWLTVSINNLSFYEKNIGSNIGYIKDYLNSELNMFTRLEINAGKRDSVDIKVNYLDLMFYYYERGIYTIKPYKALIENYLDISRETYVEAFKIASLSNGDIITKTGFQPIGYLKLVGNYENTIPSTINIVAKDTDNNPIESNELDVYNTVENRNLLQSYKGVNTIAREITSTKEFTVSGWAKEIYSTNYLSKVLKPGKVYTLSFDMEITGNDPTLKSYSDNHGIYLYSNTKGIVVNGVKSMERTIGNKVSVTQTFTAPTITDHRLLIYTGRYTSDGKASTPPVFFNTVKITELKLTEGSSKLEYSPAPEDKPNVIEKGIKFNNILTNIQTLSINSDTILKNVTLYYSYYGDSWVELKTLGNISTGETTETNNLIDLYGLQTVDYSNINPMSKVSLRSIWNVKLGELNNQEGSLSNMPNDYFNAVWQDIDKLSDIELGSMRMVKDTEGGVFDGATGEIIKATLFNVGAYTDLDMLAYTLTNYTEPLTLGSSRLISELKEELLTSESFNVDNRIKVIDSIYEELPNTSIIKNGFVEREVTGSKYLDYGLYEPIEDGTRYKLIVEGEFKDNIEFISLYNSNPNFNETFIYPSEIINGVAEKEFIAKPSTEDKPRLNTDVRIYIRPYDSTISKVRRVELRKV

Entity 10 Entity 10 polymer

Description description	Baseplate wedge subunit
Molecular weight formula_weight	26611.8 kDa
Source method src_method	nat
Copy count entity_count	2

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	L,O

MRFKKHVVQHEETMQAIAQRYYGDVSYWIDLVEHNNLKYPYLVETDEEKMKDPERLASTGDTLIIPIESDLTDVSAKEINSRDKDVLVELALGRDLNITADEKYFNEHGTSDNILAFSTNGNGDLDTVKGIDNMKQQLQARLLTPRGSLMLHPNYGSDLHNLFGLNIPEQATLIEMEVLRTLTSDNRVKSANLIDWKIQGNVYSGQFSVEIKSVEESINFVLGQDEEGIFALFE

Entity 11 Entity 11 polymer

Description description	Putative baseplate component
Molecular weight formula_weight	29381.6 kDa
Source method src_method	nat
Copy count entity_count	2

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	M,N

MPQSDGISNLHRIALRFPKEGGGYDMYRFKVNPENYTIDSPQRTTAIKTKSDIVIEDYGKDIEVINFTGTTGFRPVREADGLKTGKQKMEELQSRVSEYAMQGGSGNVSGSYLQFFNFTDDSYYKVHLAPQGLKITRSKDEPLLFRYEITLVVIGSLTEADRSAVTTEEFGNVKPNASQRVDEGIKELDKNARKTRDRNNQEISRRENTIPKSTGDNTNEGNRLKQSFPSSSIYNPRQSTNGLKGNIDNMALIIGYGDGGVSS

Entity 12 Entity 12 polymer

Description description	Major tail sheath protein
Molecular weight formula_weight	64559.0 kDa
Source method src_method	nat
Copy count entity_count	6

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	P,Q,T,U,X,Y

MAVEPFPRRPITRPHASIEVDTSGIGGSAGSSEKVFCLIGQAEGGEPNTVYELRNYSQAKRLFRSGELLDAIELAWGSNPNYTAGRILAMRIEDAKPASAEIGGLKITSKIYGNVANNIQVGLEKNTLSDSLRLRVIFQDDRFNEVYDNIGNIFTIKYKGEEANATFSVEHDEETQKASRLVLKVGDQEVKSYDLTGGAYDYTNAIITDINQLPDFEAKLSPFGDKNLESSKLDKIENANIKDKAVYVKAVFGDLEKQTAYNGIVSFEQLNAEGEVPSNVEVEAGEESATVTATSPIKTIEPFELTKLKGGTNGEPPATWADKLDKFAHEGGYYIVPLSSKQSVHAEVASFVKERSDAGEPMRAIVGGGFNESKEQLFGRQASLSNPRVSLVANSGTFVMDDGRKNHVPAYMVAVALGGLASGLEIGESITFKPLRVSSLDQIYESIDLDELNENGIISIEFVRNRTNTFFRIVDDVTTFNDKSDPVKAEMAVGEANDFLVSELKVQLEDQFIGTRTINTSASIIKDFIQSYLGRKKRDNEIQDFPAEDVQVIVEGNEARISMTVYPIRSFKKISVSLVYKQQTLQA

Entity 13 Entity 13 polymer

Description description	Capsid protein
Molecular weight formula_weight	15943.0 kDa
Source method src_method	nat
Copy count entity_count	6

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	R,S,V,W,Z,a

MASEAKQTVHTGNTVLLMIKGKPVGRAQSASGQREYGTTGVYEIGSIMPQEHVYLRYEGTITVERLRMKKENFADLGYASLGEEILKKDIIDILVVDNLTKQVIISYHGCSANNYNETWQTNEIVTEEIEFSYLTASDKART

Entity 14 Entity 14 polymer

Description description	Baseplate protein
Molecular weight formula_weight	21588.1 kDa
Source method src_method	man
Copy count entity_count	12

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	b,c,d,e,f,g,t,u,v,w,x,y

MAIATYNSHVELAKYLVSKADSVYLTIGKSTPWSNETNPPQPDENATVLQEVIGYKKATKVTLVRPSKSPEDDNKNLISYGNKSWVEVTPENAKAEGAKWVYLESSIVGDELPLGTYRQVGFVMDLVAKSGISKFNLVPSEVESTGTLLFFDNKQFQNRSEQTTAKERFIVEVDPNSSSVDKLAAALEHHHHHH

Source Source

Source organism organism	Staphylococcus phage 812
NCBI taxonomy ID ncbi_taxonomy_id	307898
Host organism host_organism	Escherichia coli BL21(DE3)

▶

Unit-cell length a length_a	1.000 Å
Unit-cell length b length_b	1.000 Å
Unit-cell length c length_c	1.000 Å
Unit-cell angle α angle_alpha	90.00 °
Unit-cell angle β angle_beta	90.00 °
Unit-cell angle γ angle_gamma	90.00 °
Molecules per unit cell Z z_pdb	—

Space group space_group	P 1
Space-group number int_tables_number	1
Crystal system crystal_system	—

Resolution resolution	7.30 Å
Resolution测定Method resolution_method	—
颗粒数 num_particles	9705

Cryo-EM structure of Staphylococcus aureus bacteriophage phi812 baseplate in the pre-contraction state - complete

1. Protein Identity and Related Structures Protein Identity & Related Structures

DUF4815 domain-containing protein

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

Receptor binding protein

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

CBM-cenC domain-containing protein

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

NlpC/P60 domain-containing protein

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

GP-PDE domain-containing protein

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

Baseplate component

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

Peptidase C51 domain-containing protein

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

Baseplate component

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

TmpF

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

Baseplate wedge subunit

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer