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9tg7

Crystal structure of beta-TrCP bound by monophosphorylated WEE1 degron peptide

Method: X-RAY DIFFRACTION D_max: 70.9 Å Quality: GOOD

SAXS Profile

P(r) Distribution

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1. Structure Basics

entry_id	9tg7
deposition_date	2025-11-28
title	Crystal structure of beta-TrCP bound by monophosphorylated WEE1 degron peptide
keywords	E3 ligase, phosphodegron, beta-TrCP, WEE1, TRANSFERASE; TRANSFERASE
method	X-RAY DIFFRACTION

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Download Data

SAXS curve (.dat) P(r) distribution (.pr.out) Structure (CIF)

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2. SAXS Parameters (CRYSOL)

rg_guinier	21.88 Å
rg_electron	21.08 Å
i0	30258700.00
molecular_weight	41325.0 kDa
excluded_volume	51287 Å³
envelope_volume	59159 Å³
shell_volume	23360 Å³
envelope_diameter	71.9 Å
shell_rg	27.93 Å
envelope_rg	21.30 Å
shape_rg	21.06 Å
total_rg	21.92 Å
total_atoms	2901
n_residues	360
n_harmonics	20
q_range	— – 0.5000 Å⁻¹
n_points	101
shell_type	directional
solvent_density	0.3340 e/Å³
contrast_shell	0.0300 e/Å³
crysol_version	4.1.3

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3. P(r) Analysis (GNOM)

dmax	70.9 Å
rg_real	21.80 Å
rg_real_error	0.38 Å
i0_real	3.0260e+07
i0_real_error	3.9150e+05
rg_reciprocal	21.82 Å
i0_reciprocal	30260000.0000
total_estimate	0.8936
solution_quality	GOOD a GOOD solution
n_peaks	2
r_peak_primary	26.4 Å
skewness	0.281
kurtosis	-0.295
angular_range	— – 0.3650 Å⁻¹
current_alpha	0.0000
highest_alpha	9797000.0000
n_real_points	68
gnom_version	4.1.3
quality_criteria	AN1: 0.000; Oscil: 0.873; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.998; Smooth: 0.997

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4. Crystallography & Experiment

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5. Entities & Polymer Info (3)

Entity 1 polymer

description	F-box/WD repeat-containing protein 1A
formula_weight	41829.7 kDa
mutation	L188E, L192E
src_method	man
entity_count	1

Polymer

type	polypeptide(L)
strand_id	A
atom_count	3095

GSGMEWKKEIERMVRTDSLWRGLAERRGWGQYLFKNKPPDGNAPPNSFYRALYPKIIQDIETIESNWRCGRHSLQRIHCRSETSKGVYCLQYDDQKIVSGLRDNTIKIWDKNTLECKRILTGHTGSVLCLQYDERVIITGSSDSTVRVWDVNTGEMLNTLIHHCEAVLHLRFNNGMMVTCSKDRSIAVWDMASPTDITLRRVLVGHRAAVNVVDFDDKYIVSASGDRTIKVWNTSTCEFVRTLNGHKRGIACLQYRDRLVVSGSSDNTIRLWDIECGACLRVLEGHEELVRCIRFDNKRIVSGAYDGKIKVWDLVAALDPRAPAGTLCLRTLVEHSGRVFRLQFDEFQIVSSSHDDTILIWDFLN

Source

organism	Homo sapiens
common_name	human
ncbi_taxonomy_id	9606
host_organism	Spodoptera frugiperda

Entity 2 polymer

description	Wee1-like protein kinase
formula_weight	1362.2 kDa
ec_number	2.7.10.2
src_method	man
entity_count	1

Polymer

type	polypeptide(L)
strand_id	B

TGEDSAFQEPDS

Source

organism	Homo sapiens
common_name	human
ncbi_taxonomy_id	9606
host_organism	synthetic construct

Entity 3 water

description	water
formula_weight	18.0 kDa
src_method	nat
entity_count	194

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6. Citations (1)

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length_a	45.894 Å
length_b	47.307 Å
length_c	148.272 Å
angle_alpha	90.00 °
angle_beta	90.00 °
angle_gamma	90.00 °
z_pdb	4

space_group	P 21 21 21
int_tables_number	19
crystal_system	—

resolution	1.684 Å
resolution_low	74.140 Å
r_factor	0.2099
r_work	0.2077
r_free	0.2520

fit	/app/data/saxs_computation/tg/9tg7/9tg7.fit
pddf	/app/data/pr_computation/tg/9tg7/9tg7.pr.out
profile	/app/data/saxs_computation/tg/9tg7/9tg7.dat

Crystal structure of beta-TrCP bound by monophosphorylated WEE1 degron peptide

SAXS Profile

P(r) Distribution

1. Structure Basics

Download Data

2. SAXS Parameters (CRYSOL)

3. P(r) Analysis (GNOM)

4. Crystallography & Experiment

Unit Cell

Symmetry

Refinement

Diffraction

5. Entities & Polymer Info (3)

Entity 1 polymer

Polymer

Source

Entity 2 polymer

Polymer

Source

Entity 3 water

6. Citations (1)

7. Files & Curves (10)

Files (7)

Curves (3)