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9uzo

Cryo-EM Structure of the Vaccinia Virus Entry/Fusion Complex (EFC) Lacking the F9 Subunit

Method: ELECTRON MICROSCOPY D_max: 199.5 Å Quality: GOOD

SAXS Profile

P(r) Distribution

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1. Structure Basics

entry_id	9uzo
deposition_date	2025-05-16
title	Cryo-EM Structure of the Vaccinia Virus Entry/Fusion Complex (EFC) Lacking the F9 Subunit
keywords	Vaccinia Virus, Entry-Fusion Complex (EFC), cryo-EM, Single-particle reconstruction, VIRAL PROTEIN; VIRAL PROTEIN
method	ELECTRON MICROSCOPY

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Download Data

SAXS curve (.dat) P(r) distribution (.pr.out) Structure (CIF)

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2. SAXS Parameters (CRYSOL)

rg_guinier	50.98 Å
rg_electron	51.89 Å
i0	860880000.00
molecular_weight	249680.0 kDa
excluded_volume	314940 Å³
envelope_volume	453140 Å³
shell_volume	76273 Å³
envelope_diameter	214.0 Å
shell_rg	51.72 Å
envelope_rg	52.11 Å
shape_rg	51.89 Å
total_rg	51.87 Å
total_atoms	17552
n_residues	2171
n_harmonics	20
q_range	— – 0.5000 Å⁻¹
n_points	101
shell_type	directional
solvent_density	0.3340 e/Å³
contrast_shell	0.0300 e/Å³
crysol_version	4.1.3

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3. P(r) Analysis (GNOM)

dmax	199.5 Å
rg_real	51.24 Å
rg_real_error	2.61 Å
i0_real	8.6090e+08
i0_real_error	1.8430e+07
rg_reciprocal	50.76 Å
i0_reciprocal	860300000.0000
total_estimate	0.8065
solution_quality	GOOD a GOOD solution
n_peaks	2
r_peak_primary	57.9 Å
skewness	0.577
kurtosis	0.190
angular_range	— – 0.1550 Å⁻¹
current_alpha	0.0002
highest_alpha	57600000.0000
n_real_points	32
gnom_version	4.1.3
quality_criteria	AN1: 0.000; Oscil: 0.586; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.751; Smooth: 0.973

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4. Crystallography & Experiment

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5. Entities & Polymer Info (9)

Entity 1 polymer

description	Virion membrane protein OPG143
formula_weight	43486.6 kDa
src_method	man
entity_count	1

Polymer

type	polypeptide(L)
strand_id	A
atom_count	17552

MGAAVTLNRIKIAPGIADIRDKYMELGFNYPEYNRAVKFAEESYTYYYETSPGEIKPKFCLIDGMSIDHCSSFIVPEFAKQYVLIHGEPCSSFKFRPGSLIYYQNEVTPEYIKDLKHATDYIASGQRCHFIKKDYLLGDSDSVAKCCSKTNTKHCPKIFNNNYKTEHCDDFMTGFCRNDPGNPNCLEWLRAKRKPAMSTYSDICSKHMDARYCSEFIRIIRPDYFTFGDTALYVFCNDHKGNRNCWCANYPKSNSGDKYLGPRVCWLHECTDESRDRKWLYYNQDVQRTRCKYVGCTINVNSLALKNSQAELTSNCTRTTSAVGDVHPGEPVVKDKIKLPTWLGAAITLVVISVIFYFISIYSRPKIKTNDINVRRR

Source

organism	Orthopoxvirus vaccinia
ncbi_taxonomy_id	10245
host_organism	Homo sapiens

Entity 2 polymer

description	Entry-fusion complex protein OPG094
formula_weight	38840.3 kDa
src_method	man
entity_count	1

Polymer

type	polypeptide(L)
strand_id	B

MGGGVSVELPKRDPPPGVPTDEMLLNVDKMHDVIAPAKLLEYVHIGPLAKDKEDKVKKRYPEFRLVNTGPGGLSALLRQSYNGTAPNCCRTFNRTHYWKKDGKISDKYEEGAVLESCWPDVHDTGKCDVDLFDWCQGDTFDRNICHQWIGSAFNRSNRTVEGQQSLINLYNKMQTLCSKDASVPICESFLHHLRAHNTEDSKEMIDYILRQQSADFKQKYMRCSYPTRDKLEESLKYAEPRECWDPECSNANVNFLLTRNYNNLGLCNIVRCNTSVNNLQMDKTSSLRLSCGLSNSDRFSTVPVNRAKVVQHNIKHSFDLKLHLISLLSLLVIWILIVAI

Source

organism	Orthopoxvirus vaccinia
ncbi_taxonomy_id	10245
host_organism	Homo sapiens

Entity 3 polymer

description	Envelope protein OPG155
formula_weight	16340.5 kDa
src_method	man
entity_count	2

Polymer

type	polypeptide(L)
strand_id	C,c

MNSLSIFFIVVATAAVCLLFIQGYSIYENYGNIKEFNATHAAFEYSKSIGGTPALDRRVQDVNDTISDVKQKWRCVVYPGNGFVSASIFGFQAEVGPNNTRSIRKFNTMQQCIDFTFSDVININIYNPCVVPNINNAECQFLKSVL

Source

organism	Orthopoxvirus vaccinia
ncbi_taxonomy_id	10245
host_organism	Homo sapiens

Entity 4 polymer

description	Protein OPG107
formula_weight	21568.9 kDa
src_method	man
entity_count	2

Polymer

type	polypeptide(L)
strand_id	D,d

MDKTTLSVNACNLEYVREKAIVGVQAAKTSTLIFFVIILAISALLLWFQTSDNPVFNELTRYMRIKNTVNDWKSLTDSKTKLESDRGRLLAAGKDDIFEFKCVDFGAYFIAMRLDKKTYLPQAIRRGTGDAWMVKKAAKVDPSAQQFCQYLIKHKSNNVITCGNEMLNELGYSGYFMSPHWCSDFSNME

Source

organism	Orthopoxvirus vaccinia
ncbi_taxonomy_id	10245
host_organism	Homo sapiens

Entity 5 polymer

description	Entry-fusion complex protein OPG086
formula_weight	12814.8 kDa
src_method	man
entity_count	2

Polymer

type	polypeptide(L)
strand_id	E,e

MASLLYLILFLLFVCISYYFTYYPTNKLQAAVMETDRENAIIRQRNDEIPTRTLDTAIFTDASTVASAQIHLYYNSNIGKIIMSLNGKKHTFNLYDDNDIRTLLPILLLSK

Source

organism	Orthopoxvirus vaccinia
ncbi_taxonomy_id	10245
host_organism	Homo sapiens

Entity 6 polymer

description	Entry-fusion complex protein OPG094
formula_weight	15062.8 kDa
src_method	man
entity_count	2

Polymer

type	polypeptide(L)
strand_id	F,f

MENVPNVYFNPVFIEPTFKHSLLSVYKHRLIVLFEVFVVFILIYVFFRSELNMFFMPKRKIPDPIDRLRRANLACEDDKLMIYGLPWMTTQTSALSINSKPIVYKDCAKLLRSINGSQPVSLNDVLRR

Source

organism	Orthopoxvirus vaccinia
ncbi_taxonomy_id	10245
host_organism	Homo sapiens

Entity 7 polymer

description	Protein OPG104
formula_weight	15174.7 kDa
src_method	man
entity_count	1

Polymer

type	polypeptide(L)
strand_id	I

MTDEQIYAFCDANKDDIRCKCIYPDKSIVRIGIDTRLPYYCWYEPCKRSDALLPASLKKNITKCNVSDCTISLGNVSITDSKLDVNNVCDSKRVATENIAVRYLNQEIRYPIIDIKWLPIGLLALAILILAFF

Source

organism	Orthopoxvirus vaccinia
ncbi_taxonomy_id	10245
host_organism	Homo sapiens

Entity 8 polymer

description	Virion membrane protein OPG147
formula_weight	13661.8 kDa
src_method	man
entity_count	2

Polymer

type	polypeptide(L)
strand_id	J,j

MITLFLILCYFILIFNIIVPAISEKMRRERAAYVNYKRLNKNFICVDDRLFSYNFTTSGIKAKVAVDNKNVPIPCSKINEVNNNKDVDTLYCDKDRDDIPGFARSCYRAYSDLFFTT

Source

organism	Orthopoxvirus vaccinia
ncbi_taxonomy_id	10245
host_organism	Homo sapiens

Entity 9 polymer

description	Entry-fusion complex protein OPG076
formula_weight	4230.1 kDa
src_method	man
entity_count	2

Polymer

type	polypeptide(L)
strand_id	K,k

MLVVIMFFIAFAFCSWLSYSYLRPYISTKELNKSR

Source

organism	Orthopoxvirus vaccinia
ncbi_taxonomy_id	10245
host_organism	Homo sapiens

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6. Citations (1)

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resolution	3.05 Å
resolution_method	—
num_particles	474787

fit	/app/data/saxs_computation/uz/9uzo/9uzo.fit
pddf	/app/data/pr_computation/uz/9uzo/9uzo.pr.out
profile	/app/data/saxs_computation/uz/9uzo/9uzo.dat

Cryo-EM Structure of the Vaccinia Virus Entry/Fusion Complex (EFC) Lacking the F9 Subunit

SAXS Profile

P(r) Distribution

1. Structure Basics

Download Data

2. SAXS Parameters (CRYSOL)

3. P(r) Analysis (GNOM)

4. Crystallography & Experiment

EM 3D

5. Entities & Polymer Info (9)

Entity 1 polymer

Polymer

Source

Entity 2 polymer

Polymer

Source

Entity 3 polymer

Polymer

Source

Entity 4 polymer

Polymer

Source

Entity 5 polymer

Polymer

Source

Entity 6 polymer

Polymer

Source

Entity 7 polymer

Polymer

Source

Entity 8 polymer

Polymer

Source

Entity 9 polymer

Polymer

Source

6. Citations (1)

7. Files & Curves (10)

Files (7)

Curves (3)