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9zbl

Helical Reconstruction of the Human Cardiac F-Actin-Tropomyosin Complex

Method: ELECTRON MICROSCOPY D_max: 199.8 Å Quality: GOOD

SAXS Profile

P(r) Distribution

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1. Structure Basics

entry_id	9zbl
deposition_date	2025-11-20
title	Helical Reconstruction of the Human Cardiac F-Actin-Tropomyosin Complex
keywords	F-actin, tropomyosin, muscle, cryo-EM structure, motor proteins, MOTOR PROTEIN; MOTOR PROTEIN
method	ELECTRON MICROSCOPY

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Download Data

SAXS curve (.dat) P(r) distribution (.pr.out) Structure (CIF)

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2. SAXS Parameters (CRYSOL)

rg_guinier	55.69 Å
rg_electron	56.27 Å
i0	1412430000.00
molecular_weight	307730.0 kDa
excluded_volume	382840 Å³
envelope_volume	542880 Å³
shell_volume	85319 Å³
envelope_diameter	216.0 Å
shell_rg	53.30 Å
envelope_rg	56.04 Å
shape_rg	56.28 Å
total_rg	56.15 Å
total_atoms	21570
n_residues	2722
n_harmonics	20
q_range	— – 0.5000 Å⁻¹
n_points	101
shell_type	directional
solvent_density	0.3340 e/Å³
contrast_shell	0.0300 e/Å³
crysol_version	4.1.3

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3. P(r) Analysis (GNOM)

dmax	199.8 Å
rg_real	56.25 Å
rg_real_error	3.12 Å
i0_real	1.4120e+09
i0_real_error	3.5570e+07
rg_reciprocal	55.22 Å
i0_reciprocal	1410000000.0000
total_estimate	0.7921
solution_quality	GOOD a GOOD solution
n_peaks	2
r_peak_primary	57.1 Å
skewness	0.647
kurtosis	-0.083
angular_range	— – 0.1400 Å⁻¹
current_alpha	0.0000
highest_alpha	135200000.0000
n_real_points	29
gnom_version	4.1.3
quality_criteria	AN1: 0.000; Oscil: 0.594; Stabil: 0.999; Sysdev: 1.000; Positv: 1.000; Valcen: 0.889; Smooth: 0.623

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4. Crystallography & Experiment

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5. Entities & Polymer Info (4)

Entity 1 polymer

description	Actin, alpha cardiac muscle 1
formula_weight	42064.9 kDa
ec_number	3.6.4.-
src_method	man
entity_count	6

Polymer

type	polypeptide(L)
strand_id	A,B,C,D,E,F
atom_count	21570

MCDDEETTALVCDNGSGLVKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPTLLTEAPLNPKANREKMTQIMFETFNVPAMYVAIQAVLSLYASGRTTGIVLDSGDGVTHNVPIYEGYALPHAIMRLDLAGRDLTDYLMKILTERGYSFVTTAEREIVRDIKEKLCYVALDFENEMATAASSSSLEKSYELPDGQVITIGNERFRCPETLFQPSFIGMESAGIHETTYNSIMKCDIDIRKDLYANNVLSGGTTMYPGIADRMQKEITALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKQEYDEAGPSIVHRKCF

Source

organism	Homo sapiens
common_name	human
ncbi_taxonomy_id	9606
host_organism	Spodoptera frugiperda

Entity 2 polymer

description	Tropomyosin alpha-1 chain
formula_weight	32921.8 kDa
src_method	man
entity_count	4

Polymer

type	polypeptide(L)
strand_id	M,N,O,P

ASMDAIKKKMQMLKLDKENALDRAEQAEADKKAAEDRSKQLEDELVSLQKKLKGTEDELDKYSEALKDAQEKLELAEKKATDAEADVASLNRRIQLVEEELDRAQERLATALQKLEEAEKAADESERGMKVIESRAQKDEEKMEIQEIQLKEAKHIAEDADRKYEEVARKLVIIESDLERAEERAELSEGKCAELEEELKTVTNNLKSLEAQAEKYSQKEDRYEEEIKVLSDKLKEAETRAEFAERSVTKLEKSIDDLEDELYAQKLKYKAISEELDHALNDMTSI

Source

organism	Homo sapiens
common_name	human
ncbi_taxonomy_id	9606
host_organism	Escherichia coli BL21(DE3)

Entity 3 non-polymer

description	ADENOSINE-5'-DIPHOSPHATE
formula_weight	427.2 kDa
src_method	syn
entity_count	6

Entity 4 non-polymer

description	MAGNESIUM ION
formula_weight	24.3 kDa
src_method	syn
entity_count	6

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6. Citations (1)

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length_a	1.000 Å
length_b	1.000 Å
length_c	1.000 Å
angle_alpha	90.00 °
angle_beta	90.00 °
angle_gamma	90.00 °
z_pdb	—

space_group	P 1
int_tables_number	1
crystal_system	—

resolution	2.790 Å
resolution_low	— Å
r_factor	—
r_work	—
r_free	—

resolution	2.79 Å
resolution_method	—
num_particles	1954406

fit	/app/data/saxs_computation/zb/9zbl/9zbl.fit
pddf	/app/data/pr_computation/zb/9zbl/9zbl.pr.out
profile	/app/data/saxs_computation/zb/9zbl/9zbl.dat

Helical Reconstruction of the Human Cardiac F-Actin-Tropomyosin Complex

SAXS Profile

P(r) Distribution

1. Structure Basics

Download Data

2. SAXS Parameters (CRYSOL)

3. P(r) Analysis (GNOM)

4. Crystallography & Experiment

Unit Cell

Symmetry

Refinement

EM 3D

5. Entities & Polymer Info (4)

Entity 1 polymer

Polymer

Source

Entity 2 polymer

Polymer

Source

Entity 3 non-polymer

Entity 4 non-polymer

6. Citations (1)

7. Files & Curves (10)

Files (7)

Curves (3)