Current Protein Identity：A8EVM5
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Difference tags compare only the current result set; every original PDB and assembly record remains separate.
Related-Structure Differences
Each row represents one biological assembly in one PDB entry; multiple monomers of the same protein are listed separately.
| PDB Entry | Assembly / Oligomeric State | Construct | Mutations and Modifications | Ligands, Ions and Non-polymers | Experimental Method | Experimental Conditions | Structure Quality |
|---|---|---|---|---|---|---|---|
| 24JS Crystal structure of voltage-gated sodium channel NavAb N49K mutant Deposited 2026-03-06 | Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain A
1–267（267 aa）
|
Mutation：N49K | 1N7 CHAPSO × 4 PX4 1,2-DIMYRISTOYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 8 LMT DODECYL-BETA-D-MALTOSIDE × 4 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；9%-11% PEG MME 2000, 100mM sodium chloride, 100mM magnesium nitrate, 25mM cadmium nitrate, 100mM Tris-HCl, pH 8.4
|
Resolution 3.50 Å R-free 0.274 |
| 24JT Crystal structure of voltage-gated sodium channel NavAb N49K mutant Deposited 2026-03-06 | Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain A
1–229（229 aa）
|
Mutation：N49K | LMT DODECYL-BETA-D-MALTOSIDE × 4 1N7 CHAPSO × 4 CA CALCIUM ION × 4 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；7%-9% PEG 6000, 100mM sodium chloride, 100mM magnesium nitrate, 100mM cadmium nitrate, 10mM copper chloride, 100mM Tris-HCl, pH 8.4
|
Resolution 4.10 Å R-free 0.375 |
| 24JU Crystal structure of voltage-gated sodium channel NavAb N49K mutant Deposited 2026-03-06 | Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain A
1–229（229 aa）
|
Mutation：N49K | LMT DODECYL-BETA-D-MALTOSIDE × 4 1N7 CHAPSO × 4 PX4 1,2-DIMYRISTOYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 32 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；7%-9% PEG 6000, 100mM sodium chloride, 100mM magnesium nitrate, 100mM cadmium nitrate, 10mM copper chloride, 100mM Tris-HCl, pH 8.4
|
Resolution 3.70 Å R-free 0.320 |
| 3RVY Crystal structure of the NavAb voltage-gated sodium channel (Ile217Cys, 2.7 A) Deposited 2011-05-06 | Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain A
1–267（267 aa）
Chain B
1–267（267 aa）
|
Mutation：I217C Mutation：I217C | PX4 1,2-DIMYRISTOYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 20 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 4.75；298 K；CHAPSO:DMPC bicelles, 2 M ammonium sulphate, 0.1 M Na-citrate pH 4.75, 28% glucose, 0.01 M YCl3, nicotinic acid (sat.), VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 2.70 Å R-free 0.273 |
| 3RVZ Crystal structure of the NavAb voltage-gated sodium channel (Ile217Cys, 2.8 A) Deposited 2011-05-06 | Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain A
1–267（267 aa）
Chain B
1–267（267 aa）
|
Mutation：I217C Mutation：I217C | PX4 1,2-DIMYRISTOYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 28 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 4.75；298 K；CHAPSO:DMPC bicelles, 2 M ammonium sulphate, 0.1 M Na-citrate pH 4.75, 28% glucose, nicotinic acid (sat.), VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 2.80 Å R-free 0.292 |
| 3RW0 Crystal structure of the NavAb voltage-gated sodium channel (Met221Cys, 2.95 A) Deposited 2011-05-06 | Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain A
1–267（267 aa）
Chain B
1–267（267 aa）
|
Mutation：M221C Non-standard monomer：Yes (specific site not provided by mmCIF) Mutation：M221C Non-standard monomer：Yes (specific site not provided by mmCIF) | PX4 1,2-DIMYRISTOYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 20 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 4.75；298 K；CHAPSO:DMPC bicelles, 2 M ammonium sulphate, 0.1 M Na-citrate pH 4.75, 28% glucose, nicotinic acid (sat.), VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 2.95 Å R-free 0.272 |
| 4EKW Crystal structure of the NavAb voltage-gated sodium channel (wild-type, 3.2 A) Deposited 2012-04-10 | Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain A
1–267（267 aa）
Chain B
1–267（267 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | PX4 1,2-DIMYRISTOYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 6 PO4 PHOSPHATE ION × 4 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 4.75；298 K；CHAPSO:DMPC BICELLES, 2 M AMMONIUM SULPHATE, 0.1 M NA-CITRATE, 28% GLUCOSE, NICOTINIC ACID, pH 4.75, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 3.21 Å R-free 0.322 |
| 4EKW Crystal structure of the NavAb voltage-gated sodium channel (wild-type, 3.2 A) Deposited 2012-04-10 | Assembly 2 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain C
1–267（267 aa）
Chain D
1–267（267 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | PX4 1,2-DIMYRISTOYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 PO4 PHOSPHATE ION × 4 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 4.75；298 K；CHAPSO:DMPC BICELLES, 2 M AMMONIUM SULPHATE, 0.1 M NA-CITRATE, 28% GLUCOSE, NICOTINIC ACID, pH 4.75, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 3.21 Å R-free 0.322 |
| 4MS2 Structural basis of Ca2+ selectivity of a voltage-gated calcium channel Deposited 2013-09-18 | Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain A
1–219（219 aa）
Chain B
1–219（219 aa）
Chain C
1–219（219 aa）
Chain D
1–219（219 aa）
|
Not recorded | PX4 1,2-DIMYRISTOYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 20 CA CALCIUM ION × 5 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 4.75；298 K；0.1M Na-citrate, pH 4.75, 2M ammonium sulfate, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 2.75 Å R-free 0.255 |
| 4MTF Structural Basis of Ca2+ Selectivity of a Voltage-gated Calcium Channel Deposited 2013-09-19 | Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain A
1–219（219 aa）
Chain B
1–219（219 aa）
Chain C
1–219（219 aa）
Chain D
1–219（219 aa）
|
Not recorded | CA CALCIUM ION × 2 PX4 1,2-DIMYRISTOYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 16 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 4.75；298 K；0.1M Na-citrate,pH4.75, 2M ammonium sulfate, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 3.20 Å R-free 0.272 |
| 4MTG Structural Basis for Ca2+ Selectivity of a Voltage-gated Calcium Channel Deposited 2013-09-19 | Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain A
1–219（219 aa）
Chain B
1–219（219 aa）
Chain C
1–219（219 aa）
Chain D
1–219（219 aa）
|
Not recorded | CA CALCIUM ION × 2 PX4 1,2-DIMYRISTOYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 16 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 4.75；298 K；0.1M Na-citrate,pH 4.75, 2M ammonium sulfate, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 3.30 Å R-free 0.273 |
| 4MTO Structural Basis for Ca2+ Selectivity of a Voltage-gated Calcium Channel Deposited 2013-09-19 | Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain A
1–219（219 aa）
Chain B
1–219（219 aa）
Chain C
1–219（219 aa）
Chain D
1–219（219 aa）
|
Not recorded | CA CALCIUM ION × 3 PX4 1,2-DIMYRISTOYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 16 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 4.75；298 K；0.1M Na-citrate,pH 4.75, 2M ammonium sulfate, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 3.40 Å R-free 0.297 |
| 4MVM Structural Basis for Ca2+ Selectivity of a Voltage-gated Calcium Channel Deposited 2013-09-24 | Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain A
1–219（219 aa）
Fragment：Voltage-gated Calcium Channel (Residues 1-219)
Chain B
1–219（219 aa）
Fragment：Voltage-gated Calcium Channel (Residues 1-219)
Chain C
1–219（219 aa）
Fragment：Voltage-gated Calcium Channel (Residues 1-219)
Chain D
1–219（219 aa）
Fragment：Voltage-gated Calcium Channel (Residues 1-219)
|
Not recorded | PX4 1,2-DIMYRISTOYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 16 CA CALCIUM ION × 5 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 4.75；298 K；CHAPSO:DMPC BICELLES,0.1M Na-Acetate ,pH4.75, 2M Ammonium Sulfate, 28% Glucose, 10mM Calcium Chloride., VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 3.20 Å R-free 0.275 |
| 4MVO Structural Basis for Ca2+ Selectivity of a Voltage-gated Calcium Channel Deposited 2013-09-24 | Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain A
1–219（219 aa）
Fragment：Voltage-gated Calcium Channel (Residues 1-219)
Chain B
1–219（219 aa）
Fragment：Voltage-gated Calcium Channel (Residues 1-219)
Chain C
1–219（219 aa）
Fragment：Voltage-gated Calcium Channel (Residues 1-219)
Chain D
1–219（219 aa）
Fragment：Voltage-gated Calcium Channel (Residues 1-219)
|
Not recorded | PX4 1,2-DIMYRISTOYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 16 CA CALCIUM ION × 3 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 4.75；298 K；CHAPSO:DMPC BICELLES, 0.1M Na-Acetate, pH4.75, 2M Ammonium Sulfate, 28% Glucose, 15mM Calcium Chloride., VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 3.30 Å R-free 0.279 |
| 4MVQ Structural Basis for Ca2+ Selectivity of a Voltage-gated Calcium Channel Deposited 2013-09-24 | Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain A
1–219（219 aa）
Chain B
1–219（219 aa）
Chain C
1–219（219 aa）
Chain D
1–219（219 aa）
|
Not recorded | CA CALCIUM ION × 2 PX4 1,2-DIMYRISTOYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 16 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 4.75；298 K；CHAPSO:DMPC BICELLES,0.1M Na-Acetate ,pH4.75, 2M Ammonium Sulfate, 28% Glucose, 15mM Calcium Chloride., VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 3.40 Å R-free 0.313 |
| 4MVR Structural Basis for Ca2+ Selectivity of a Voltage-gated Calcium Channel Deposited 2013-09-24 | Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain A
1–219（219 aa）
Chain B
1–219（219 aa）
Chain C
1–219（219 aa）
Chain D
1–219（219 aa）
|
Not recorded | MN MANGANESE (II) ION × 1 PX4 1,2-DIMYRISTOYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 16 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5；298 K；CHAPSO:DMPC BICELLES, 0.1M Na-Acetate, pH5.0, 2M Ammonium Sulfate, 28% Glucose, 10mM Mn2+, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 3.20 Å R-free 0.262 |
| 4MVS Structural Basis for Ca2+ Selectivity of a Voltage-gated Calcium Channel Deposited 2013-09-24 | Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain A
1–219（219 aa）
Chain B
1–219（219 aa）
Chain C
1–219（219 aa）
Chain D
1–219（219 aa）
|
Not recorded | CD CADMIUM ION × 5 PX4 1,2-DIMYRISTOYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 16 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 4.75；298 K；CHAPSO:DMPC bicelles, 0.1M Na-Acetate, pH4.75, 2M Ammonium Sulfate, 28% Glucose, 100mM Mn2+, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 3.30 Å R-free 0.269 |
| 4MVU Structural Basis for Ca2+ Selectivity of a Voltage-gated Calcium Channel Deposited 2013-09-24 | Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain A
1–219（219 aa）
Chain B
1–219（219 aa）
Chain C
1–219（219 aa）
Chain D
1–219（219 aa）
|
Not recorded | PX4 1,2-DIMYRISTOYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 16 CA CALCIUM ION × 3 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 4.75；298 K；CHAPSO:DMPC bicelles, 0.1M Na-Acetate, pH4.75, 2M Ammonium Sulfate, 28% Glucose, 15mM Calcium Chloride, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 3.20 Å R-free 0.263 |
| 4MVZ Structural Basis for Ca2+ Selectivity of a Voltage-gated Calcium Channel Deposited 2013-09-24 | Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain A
1–219（219 aa）
Chain B
1–219（219 aa）
Chain C
1–219（219 aa）
Chain D
1–219（219 aa）
|
Not recorded | CA CALCIUM ION × 2 PX4 1,2-DIMYRISTOYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 16 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 4.75；298 K；CHAPSO:DMPC bicelles, 0.1M Na-Acetate, pH 4.75, 2M Ammonium Sulfate, 28% Glucose, 15mM Calcium Chloride, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 3.30 Å R-free 0.280 |
| 4MW3 Structural Basis for Ca2+ Selectivity of a Voltage-gated Calcium Channel Deposited 2013-09-24 | Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain A
1–219（219 aa）
Chain B
1–219（219 aa）
Chain C
1–219（219 aa）
Chain D
1–219（219 aa）
|
Not recorded | CA CALCIUM ION × 3 PX4 1,2-DIMYRISTOYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 16 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 4.75；298 K；CHAPSO:DMPC bicelles, 0.1M Na-Acetate,pH 4.75, 2M Ammonium Sulfate, 28% Glucose, 15mM Calcium Chloride, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 3.30 Å R-free 0.267 |
| 4MW8 Structural Basis for Ca2+ Selectivity of a Voltage-gated Calcium Channel Deposited 2013-09-24 | Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain A
1–219（219 aa）
Chain B
1–219（219 aa）
Chain C
1–219（219 aa）
Chain D
1–219（219 aa）
|
Not recorded | CA CALCIUM ION × 2 PX4 1,2-DIMYRISTOYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 16 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 4.75；298 K；CHAPSO:DMPC bicelles, 0.1M Na-Acetate,pH 4.75, 2M Ammonium Sulfate, 28% Glucose, 15mM Calcium Chloride, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 3.26 Å R-free 0.314 |
| 5EK0 Human Nav1.7-VSD4-NavAb in complex with GX-936. Deposited 2015-11-03 | Assembly 1 Insufficient information Homooligomer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain A
1–23（23 aa）
Chain A
58–78（21 aa）
Chain A
109–267（159 aa）
Chain B
1–23（23 aa）
Chain B
58–78（21 aa）
Chain B
109–267（159 aa）
Chain C
1–23（23 aa）
Chain C
58–78（21 aa）
Chain C
109–267（159 aa）
Chain D
1–23（23 aa）
Chain D
58–78（21 aa）
Chain D
109–267（159 aa）
|
Not recorded | PX4 1,2-DIMYRISTOYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 21 5P2 3-cyano-4-[2-[2-(1-ethylazetidin-3-yl)pyrazol-3-yl]-4-(trifluoromethyl)phenoxy]-~{N}-(1,2,4-thiadiazol-5-yl)benzenesulfonamide × 4 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 5.4；277 K；2.2 ammonium sulfate, 100 mM sodium citrate pH 5.4
|
Resolution 3.53 Å R-free 0.272 |
| 5KLB Crystal structure of the CavAb voltage-gated calcium channel(wild-type, 2.7A) Deposited 2016-06-23 | Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain A
1–267（267 aa）
Chain B
1–267（267 aa）
Chain C
1–267（267 aa）
Chain D
1–267（267 aa）
|
Not recorded | MC3 1,2-DIMYRISTOYL-RAC-GLYCERO-3-PHOSPHOCHOLINE × 20 CA CALCIUM ION × 3 CPS 3-[(3-CHOLAMIDOPROPYL)DIMETHYLAMMONIO]-1-PROPANESULFONATE × 6 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5；298 K；CHAPSO:DMPC BICELLES,0.1M Na-citrate,pH5.0,2M Ammonium Sulfate
|
Resolution 2.70 Å R-free 0.260 |
| 5KLG Structure of CavAb(W195Y) in complex with Br-dihydropyridine derivative UK-59811 Deposited 2016-06-24 | Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain A
1–267（267 aa）
Chain B
1–267（267 aa）
Chain C
1–267（267 aa）
Chain D
1–267（267 aa）
|
Not recorded | CA CALCIUM ION × 2 MC3 1,2-DIMYRISTOYL-RAC-GLYCERO-3-PHOSPHOCHOLINE × 11 6UC O3-ethyl O5-methyl (4R)-4-(2-bromophenyl)-2-[2-(dimethylamino)ethoxymethyl]-6-methyl-1,4-dihydropyridine-3,5-dicarboxylate × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5；298 K；CHAPSO:DMPC BICELLES,0.1M Na-citrate,pH5.0,2M Ammonium Sulfate
|
Resolution 3.30 Å R-free 0.303 |
| 5KLS Structure of CavAb in complex with Br-dihydropyridine derivative UK-59811 Deposited 2016-06-25 | Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain A
1–267（267 aa）
Chain B
1–267（267 aa）
Chain C
1–267（267 aa）
Chain D
1–267（267 aa）
|
Not recorded | MC3 1,2-DIMYRISTOYL-RAC-GLYCERO-3-PHOSPHOCHOLINE × 15 CA CALCIUM ION × 2 6UC O3-ethyl O5-methyl (4R)-4-(2-bromophenyl)-2-[2-(dimethylamino)ethoxymethyl]-6-methyl-1,4-dihydropyridine-3,5-dicarboxylate × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5；298 K；CHAPSO:DMPC BICELLES,0.1M Na-citrate,pH5.0,2M Ammonium Sulfate
100uM UK-59811
|
Resolution 3.30 Å R-free 0.300 |
| 5KMD Structure of CavAb in complex with amlodipine Deposited 2016-06-26 | Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain A
1–267（267 aa）
Chain B
1–267（267 aa）
Chain C
1–267（267 aa）
Chain D
1–267（267 aa）
|
Not recorded | MC3 1,2-DIMYRISTOYL-RAC-GLYCERO-3-PHOSPHOCHOLINE × 14 CA CALCIUM ION × 2 6UB amlodipine × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5；298 K；CHAPSO:DMPC BICELLES,0.1M Na-citrate,pH5.0,2M Ammonium Sulfate
,100uM amlodipine
|
Resolution 3.20 Å R-free 0.277 |
| 5KMF Structure of CavAb in complex with nimodipine Deposited 2016-06-26 | Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain A
1–267（267 aa）
Chain B
1–267（267 aa）
Chain C
1–267（267 aa）
Chain D
1–267（267 aa）
|
Not recorded | 6U9 S-nimodipine × 1 MC3 1,2-DIMYRISTOYL-RAC-GLYCERO-3-PHOSPHOCHOLINE × 14 CA CALCIUM ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5；298 K；CHAPSO:DMPC BICELLES,0.1M Na-citrate,pH5.0,2M Ammonium Sulfate,100uM nimodipine
|
Resolution 3.20 Å R-free 0.256 |
| 5KMH Structure of CavAb in complex with Br-verapamil Deposited 2016-06-27 | Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain A
1–267（267 aa）
Chain B
1–267（267 aa）
Chain C
1–267（267 aa）
Chain D
1–267（267 aa）
|
Not recorded | CA CALCIUM ION × 3 MC3 1,2-DIMYRISTOYL-RAC-GLYCERO-3-PHOSPHOCHOLINE × 6 PX6 1,2-DIPALMITOYL-SN-GLYCERO-3-PHOSPHATE × 4 6U8 (2~{R})-2-(2-bromophenyl)-5-[2-(3,4-dimethoxyphenyl)ethyl-methyl-amino]-2-propan-2-yl-pentanenitrile × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5；298 K；CHAPSO:DMPC BICELLES,0.1M Na-citrate,pH5.0,2M Ammonium Sulfate,100uM Br-verapamil
|
Resolution 3.20 Å R-free 0.294 |
| 5VB2 Crystal structure of the NavAb voltage-gated sodium channel in a closed conformation Deposited 2017-03-28 | Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain A
1–267（267 aa）
Chain B
1–267（267 aa）
Chain C
1–267（267 aa）
Chain D
1–267（267 aa）
|
Mutation：T206F, V213Y Mutation：T206F, V213Y Mutation：T206F, V213Y Mutation：T206F, V213Y | CPS 3-[(3-CHOLAMIDOPROPYL)DIMETHYLAMMONIO]-1-PROPANESULFONATE × 8 PX4 1,2-DIMYRISTOYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 13 PO4 PHOSPHATE ION × 4 CL CHLORIDE ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；277.15 K；1.8 M ammonium sulfate and 100 mM sodium acetate, 1,2-dimyristoyl-sn-glycero-3-phosphatidylcholine (DMPC):CHAPSO bicelles
|
Resolution 3.20 Å R-free 0.266 |
| 5VB8 Crystal structure of the NavAb voltage-gated sodium channel in an open state Deposited 2017-03-28 | Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain A
1–226（226 aa）
Fragment：UNP residues 1-226
|
Not recorded | NA SODIUM ION × 4 PX4 1,2-DIMYRISTOYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 16 SO4 SULFATE ION × 4 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 4.8；277.15 K；1.8 M ammonium sulfate and 100 mM sodium acetate (pH 4.8). 1,2-dimyristoyl-sn-glycero-3-phosphatidylcholine (DMPC):CHAPSO bicelles (Anatrace)
|
Resolution 2.85 Å R-free 0.254 |
| 6C1E NavAb NormoPP mutant Deposited 2018-01-04 | Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain A
1–267（267 aa）
Chain B
1–267（267 aa）
|
Mutation：C235I, H123R Mutation：C235I, H123R | PX4 1,2-DIMYRISTOYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 36 BNC 5-BETA-24-NOR-CHOLANE-3(ALPHA),7(ALPHA),12(ALPHA)-TRIOL × 2 UHH (3ALPHA,5ALPHA,7ALPHA,8ALPHA,12ALPHA,14BETA,17ALPHA)-3,7,12-TRIHYDROXYCHOL-1-EN-24-AMIDE × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
EVAPORATION；277 K；ammonium sulphate, Na-citrate
|
Resolution 2.86 Å R-free 0.240 |
| 6C1K HypoPP mutant with ligand1 Deposited 2018-01-04 | Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain A
1–267（267 aa）
Chain B
1–267（267 aa）
|
Mutation：C235I, H123R Mutation：C235I, H123R | GAI GUANIDINE × 4 PX4 1,2-DIMYRISTOYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 34 NA SODIUM ION × 8 SO4 SULFATE ION × 4 | X-RAY DIFFRACTION |
X-ray crystallization conditions
EVAPORATION；277 K；ammonium sulphate, Na-citrate
|
Resolution 2.70 Å R-free 0.246 |
| 6C1M NavAb NormoPP mutant Deposited 2018-01-04 | Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain A
1–267（267 aa）
Chain B
1–267（267 aa）
|
Mutation：C235I, H123R Mutation：C235I, H123R | PX4 1,2-DIMYRISTOYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 30 1N7 CHAPSO × 16 MGX 1-METHYLGUANIDINE × 4 NA SODIUM ION × 8 SO4 SULFATE ION × 4 | X-RAY DIFFRACTION |
X-ray crystallization conditions
EVAPORATION；pH 4.8；277 K；ammonium sulphate, Na-citrate
|
Resolution 2.52 Å R-free 0.227 |
| 6C1P HypoPP mutant Deposited 2018-01-05 | Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain A
1–267（267 aa）
Chain B
1–267（267 aa）
Chain C
1–267（267 aa）
Chain D
1–267（267 aa）
|
Mutation：C235I, H123R Mutation：C235I, H123R Mutation：C235I, H123R Mutation：C235I, H123R | PO4 PHOSPHATE ION × 2 PX4 1,2-DIMYRISTOYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 15 1N7 CHAPSO × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
EVAPORATION；277 K；ammonium sulphate, Na-citrate
|
Resolution 2.90 Å R-free 0.260 |
| 6JUH structure of CavAb in complex with efonidipine Deposited 2019-04-13 | Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain A
1–267（267 aa）
Chain B
1–267（267 aa）
Chain C
1–267（267 aa）
Chain D
1–267（267 aa）
|
Mutation：E1177D, S1178D, M1181N Mutation：E1177D, S1178D, M1181N Mutation：E1177D, S1178D, M1181N Mutation：E1177D, S1178D, M1181N | MC3 1,2-DIMYRISTOYL-RAC-GLYCERO-3-PHOSPHOCHOLINE × 4 PX4 1,2-DIMYRISTOYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 G3P SN-GLYCEROL-3-PHOSPHATE × 4 CA CALCIUM ION × 2 C9X 2-[phenyl-(phenylmethyl)amino]ethyl (4~{R})-5-(5,5-dimethyl-2-oxidanylidene-1,3,2$l^{5}-dioxaphosphinan-2-yl)-2,6-dimethyl-4-(3-nitrophenyl)-1,4-dihydropyridine-3-carboxylate × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；277.15 K；1.0-2.0M Ammonium Sulfate
100 mM Na-citrate pH=5.0
|
Resolution 3.00 Å R-free 0.291 |
| 6KE5 Structure of CavAb in complex with Diltiazem and Amlodipine Deposited 2019-07-03 | Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain A
1–267（267 aa）
Chain B
1–267（267 aa）
Chain C
1–267（267 aa）
Chain D
1–267（267 aa）
|
Not recorded | LPC [1-MYRISTOYL-GLYCEROL-3-YL]PHOSPHONYLCHOLINE × 3 CA CALCIUM ION × 3 G3P SN-GLYCEROL-3-PHOSPHATE × 5 6UB amlodipine × 1 D6C [(2~{S},3~{R})-5-[2-(dimethylamino)ethyl]-2-(4-methoxyphenyl)-4-oxidanylidene-2,3-dihydro-1,5-benzothiazepin-3-yl] ethanoate × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5；298 K；0.1M Na-citrate,pH5.0,2M Ammonium Sulfate
|
Resolution 2.80 Å R-free 0.292 |
| 6KEB Structure basis for Diltiazem block of a voltage-gated calcium channel Deposited 2019-07-04 | Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain A
1–267（267 aa）
Chain B
1–267（267 aa）
Chain C
1–267（267 aa）
Chain D
1–267（267 aa）
|
Not recorded | PX4 1,2-DIMYRISTOYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 12 CA CALCIUM ION × 2 D6C [(2~{S},3~{R})-5-[2-(dimethylamino)ethyl]-2-(4-methoxyphenyl)-4-oxidanylidene-2,3-dihydro-1,5-benzothiazepin-3-yl] ethanoate × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5；298 K；CHAPSO:DMPC BICELLES,0.1M Na-citrate,pH5.0,2M Ammonium Sulfate
|
Resolution 3.20 Å R-free 0.277 |
| 6MVV NavAb voltage-gated sodium channel, I217C/F203A Deposited 2018-10-28 | Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain A
1–267（267 aa）
Chain B
1–267（267 aa）
Chain C
1–267（267 aa）
Chain D
1–267（267 aa）
|
Mutation：F203A, I217C Mutation：F203A, I217C Mutation：F203A, I217C Mutation：F203A, I217C | PX4 1,2-DIMYRISTOYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 6 PO4 PHOSPHATE ION × 5 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；277.15 K；1.8 M Ammonium Sulfate
100mM Sodium Acetate, pH 5.0
|
Resolution 2.90 Å R-free 0.256 |
| 6MVW NavAb voltage-gated sodium channel, I217C/F203W Deposited 2018-10-28 | Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain A
1–267（267 aa）
Chain B
1–267（267 aa）
Chain C
1–267（267 aa）
Chain D
1–267（267 aa）
|
Mutation：I217C, F203W Mutation：I217C, F203W Mutation：I217C, F203W Mutation：I217C, F203W | PX4 1,2-DIMYRISTOYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 10 PO4 PHOSPHATE ION × 8 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；277 K；1.8 M Ammonium Sulfate, 100 mM Sodium Acetate
|
Resolution 3.20 Å R-free 0.246 |
| 6MVX NavAb Voltage-gated Sodium Channel, I217C, in Complex with Class 1C Anti-arrhythmic Flecainide Deposited 2018-10-28 | Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain A
1–267（267 aa）
Chain B
1–267（267 aa）
Chain C
1–267（267 aa）
Chain D
1–267（267 aa）
|
Mutation：I217C Mutation：I217C Mutation：I217C Mutation：I217C | PO4 PHOSPHATE ION × 10 K4D Flecainide × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；277 K；1.8 M Ammonium Sulfate
100 mM Sodium Acetate, pH 5.0
100 uM Flecainide
|
Resolution 3.46 Å R-free 0.263 |
| 6MVY NavAb voltage-gated sodium channel, residues 1-226, crystallized in the presence of Class 1B Anti-arrhythmic drug Lidocaine Deposited 2018-10-28 | Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain A
1–226（226 aa）
|
Mutation：I217C | PX4 1,2-DIMYRISTOYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 16 SO4 SULFATE ION × 4 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；277 K；1.8 M Ammonium Sulfate
100 mM Sodium Acetate, pH 5.0
10 mM Lidocaine
|
Resolution 3.00 Å R-free 0.237 |
| 6MVY NavAb voltage-gated sodium channel, residues 1-226, crystallized in the presence of Class 1B Anti-arrhythmic drug Lidocaine Deposited 2018-10-28 | Assembly 2 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain B
1–226（226 aa）
|
Mutation：I217C | PX4 1,2-DIMYRISTOYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 16 SO4 SULFATE ION × 4 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；277 K；1.8 M Ammonium Sulfate
100 mM Sodium Acetate, pH 5.0
10 mM Lidocaine
|
Resolution 3.00 Å R-free 0.237 |
| 6MWA NavAb Voltage-gated Sodium Channel, residues 1-239 Deposited 2018-10-29 | Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain B
1–239（239 aa）
|
Not recorded | PX4 1,2-DIMYRISTOYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 16 SO4 SULFATE ION × 4 CPS 3-[(3-CHOLAMIDOPROPYL)DIMETHYLAMMONIO]-1-PROPANESULFONATE × 8 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5.8；277 K；1.8 M Ammonium Sulfate
100mM Sodium Acetate pH 5.8
|
Resolution 2.40 Å R-free 0.249 |
| 6MWB NavAb Voltage-gated Sodium Channel, residues 1-239 with mutation T206A Deposited 2018-10-29 | Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain B
1–239（239 aa）
|
Mutation：T206A | PX4 1,2-DIMYRISTOYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 16 SO4 SULFATE ION × 4 CPS 3-[(3-CHOLAMIDOPROPYL)DIMETHYLAMMONIO]-1-PROPANESULFONATE × 8 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5.8；277 K；1.8 M Ammonium Sulfate
100 mM Sodium Acetate pH 5.8
|
Resolution 2.60 Å R-free 0.236 |
| 6MWD NavAb Voltage-gated Sodium Channel, residues 1-239 with mutation T206S Deposited 2018-10-29 | Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain B
1–239（239 aa）
|
Mutation：T206S | PX4 1,2-DIMYRISTOYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 16 ACT ACETATE ION × 4 SO4 SULFATE ION × 4 CPS 3-[(3-CHOLAMIDOPROPYL)DIMETHYLAMMONIO]-1-PROPANESULFONATE × 12 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5.8；277 K；1.8 Ammonium Sulfate
100 mM Sodium Acetate, pH 5.8
|
Resolution 2.33 Å R-free 0.250 |
| 6MWG NavAb Voltage-gated Sodium Channel, residues 1-239, with mutation T206V Deposited 2018-10-29 | Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain B
1–239（239 aa）
|
Mutation：T206V | PX4 1,2-DIMYRISTOYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 16 ACT ACETATE ION × 4 CPS 3-[(3-CHOLAMIDOPROPYL)DIMETHYLAMMONIO]-1-PROPANESULFONATE × 8 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5.8；277 K；1.8 M Ammonium Sulfate
100 mM Sodium Acetate, pH 5.8
|
Resolution 2.50 Å R-free 0.239 |
| 6N4Q CryoEM structure of Nav1.7 VSD2 (actived state) in complex with the gating modifier toxin ProTx2 Deposited 2018-11-20 | Assembly 1 Insufficient information Heteromer；Protein × 12 PDB declaration: dodecameric（12） Consistent with protein count |
Chain A
1–25（25 aa）
Chain A
58–78（21 aa）
Chain A
107–267（161 aa）
Chain B
1–25（25 aa）
Chain B
58–78（21 aa）
Chain B
107–267（161 aa）
Chain C
1–25（25 aa）
Chain C
58–78（21 aa）
Chain C
107–267（161 aa）
Chain D
1–25（25 aa）
Chain D
58–78（21 aa）
Chain D
107–267（161 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 8；10 mM Tris pH 8.0, 100 mM NaCl, 0.06% FA3, 0.1 mg/ml POPC:POPE:POPG mixed at molar ratio 3:1:1
cryo-EM vitrification conditions
Cryogen ETHANE；Apply 3 uL, blot 2.5s. Ted Pella 595 filter paper.
|
Resolution 3.60 Å |
| 6N4R CryoEM structure of Nav1.7 VSD2 (deactived state) in complex with the gating modifier toxin ProTx2 Deposited 2018-11-20 | Assembly 1 Insufficient information Heteromer；Protein × 12 PDB declaration: dodecameric（12） Consistent with protein count |
Chain A
1–25（25 aa）
Chain A
58–78（21 aa）
Chain A
107–267（161 aa）
Chain B
1–25（25 aa）
Chain B
58–78（21 aa）
Chain B
107–267（161 aa）
Chain C
1–25（25 aa）
Chain C
58–78（21 aa）
Chain C
107–267（161 aa）
Chain D
1–25（25 aa）
Chain D
58–78（21 aa）
Chain D
107–267（161 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 8；10 mM Tris pH 8.0, 100 mM NaCl, 0.06% FA3, 0.1 mg/ml POPC:POPE:POPG mixed at molar ratio 3:1:1
cryo-EM vitrification conditions
Cryogen ETHANE；Apply 3 uL, blot 2.5s. Ted Pella 595 filter paper.
|
Resolution 4.20 Å |
| 6P6W Cryo-EM structure of voltage-gated sodium channel NavAb N49K/L109A/M116V/G94C/Q150C disulfide crosslinked mutant in the resting state Deposited 2019-06-04 | Assembly 1 Insufficient information Homooligomer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain A
1–267（267 aa）
Chain B
1–267（267 aa）
Chain C
1–267（267 aa）
Chain D
1–267（267 aa）
|
Mutation：R4A, N49K, L109A, M116V, G94C, Q150C,R4A, N49K, L109A, M116V, G94C, Q150C Mutation：R4A, N49K, L109A, M116V, G94C, Q150C,R4A, N49K, L109A, M116V, G94C, Q150C Mutation：R4A, N49K, L109A, M116V, G94C, Q150C,R4A, N49K, L109A, M116V, G94C, Q150C Mutation：R4A, N49K, L109A, M116V, G94C, Q150C,R4A, N49K, L109A, M116V, G94C, Q150C | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.00 Å |
| 6P6X Crystal structure of voltage-gated sodium channel NavAb G94C/Q150C mutant in the activated state Deposited 2019-06-04 | Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain A
1–239（239 aa）
|
Mutation：G94C, Q150C | PX4 1,2-DIMYRISTOYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 36 CPS 3-[(3-CHOLAMIDOPROPYL)DIMETHYLAMMONIO]-1-PROPANESULFONATE × 8 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5.6；277 K；1.8 M ammonium sulfate
0.1 M sodium citrate pH 5.6
|
Resolution 2.75 Å R-free 0.265 |
| 6P6Y Crystal structure of voltage-gated sodium channel NavAb V100C/Q150C disulfide crosslinked mutant in the activated state Deposited 2019-06-04 | Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain A
1–239（239 aa）
|
Mutation：V100C, Q150C | CPS 3-[(3-CHOLAMIDOPROPYL)DIMETHYLAMMONIO]-1-PROPANESULFONATE × 8 PX4 1,2-DIMYRISTOYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 20 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 4.8；277 K；1.8 M ammonium sulfate
0.1 M sodium citrate pH 4.8
|
Resolution 2.89 Å R-free 0.273 |
| 7K48 Structure of NavAb/Nav1.7-VS2A chimera trapped in the resting state by tarantula toxin m3-Huwentoxin-IV Deposited 2020-09-15 | Assembly 1 Insufficient information Heteromer；Protein × 8 PDB declaration: octameric（8） Consistent with protein count |
Chain A
1–17（17 aa）
Chain A
60–83（24 aa）
Chain A
106–239（134 aa）
Chain B
1–17（17 aa）
Chain B
60–83（24 aa）
Chain B
106–239（134 aa）
Chain C
1–17（17 aa）
Chain C
60–83（24 aa）
Chain C
106–239（134 aa）
Chain D
1–17（17 aa）
Chain D
60–83（24 aa）
Chain D
106–239（134 aa）
|
Mutation：R398A,L506A,M513V Mutation：R398A,L506A,M513V Mutation：R398A,L506A,M513V Mutation：R398A,L506A,M513V Mutation：R398A,L506A,M513V Mutation：R398A,L506A,M513V Mutation：R398A,L506A,M513V Mutation：R398A,L506A,M513V Mutation：R398A,L506A,M513V Mutation：R398A,L506A,M513V Mutation：R398A,L506A,M513V Mutation：R398A,L506A,M513V | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE；Blot for 2.5-4.0 seconds before plunging
|
Resolution 3.60 Å |
| 8DIV Crystal structure of NavAb I22V as a basis for the human Nav1.7 Inherited Erythromelalgia I136V mutation Deposited 2022-06-29 | Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain A
1–239（239 aa）
|
Mutation：I22V | BGC beta-D-glucopyranose × 4 PX4 1,2-DIMYRISTOYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 20 CPS 3-[(3-CHOLAMIDOPROPYL)DIMETHYLAMMONIO]-1-PROPANESULFONATE × 8 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5；277 K；1.7-1.8 M Ammonium sulfate
0.1 M Sodium Citrate pH 5.0
|
Resolution 2.54 Å R-free 0.237 |
| 8DIW Crystal structure of NavAb E96P as a basis for the human Nav1.7 Inherited Erythromelalgia S211P mutation Deposited 2022-06-29 | Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain A
1–239（239 aa）
|
Mutation：E96P | PX4 1,2-DIMYRISTOYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 20 CPS 3-[(3-CHOLAMIDOPROPYL)DIMETHYLAMMONIO]-1-PROPANESULFONATE × 8 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5.4；277 K；1.7-1.8 M Ammonium Sulfate
0.1 M Sodium Citrate pH 5.4
|
Resolution 3.11 Å R-free 0.272 |
| 8DIX Structure of NavAb L98R as a basis for the human Nav1.7 Inherited Erythromelalgia L823R mutation Deposited 2022-06-29 | Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain A
1–239（239 aa）
|
Mutation：L98R | PX4 1,2-DIMYRISTOYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 20 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 4.8；277 K；1.8-1.9 M Ammonium Sulfate
0.1 M Sodium Citrate pH 4.8
|
Resolution 3.30 Å R-free 0.255 |
| 8DIY Crystal structure of NavAb L101S as a basis for the human Nav1.7 Inherited Erythromelalgia F216S mutation Deposited 2022-06-29 | Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain A
1–239（239 aa）
|
Mutation：L101S | PX4 1,2-DIMYRISTOYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 20 CPS 3-[(3-CHOLAMIDOPROPYL)DIMETHYLAMMONIO]-1-PROPANESULFONATE × 8 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 4.8；277 K；1.7-1.8 M Ammonium Sulfate
0.1 M Sodium Citrate pH 4.8
|
Resolution 2.85 Å R-free 0.254 |
| 8DIZ Crystal structure of NavAb I119T as a basis for the human Nav1.7 Inherited Erythromelalgia I234T mutation Deposited 2022-06-29 | Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain A
1–239（239 aa）
|
Mutation：I119T | PX4 1,2-DIMYRISTOYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 20 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 4.6；277 K；1.8-1.9 M Ammonium Sulfate
0.1 M Sodium Citrate pH 4.6
|
Resolution 2.75 Å R-free 0.255 |
| 8DJ0 Crystal structure of NavAb L123T as a basis for the human Nav1.7 Inherited Erythromelalgia I848T mutation Deposited 2022-06-29 | Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain A
1–239（239 aa）
|
Mutation：L123T | PX4 1,2-DIMYRISTOYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 20 CPS 3-[(3-CHOLAMIDOPROPYL)DIMETHYLAMMONIO]-1-PROPANESULFONATE × 8 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 4.6；277 K；1.9 M Ammonium sulfate
0.1 M Sodium Citrate pH 4.6
|
Resolution 2.70 Å R-free 0.248 |
| 8DJ1 Crystal structure of NavAb V126T as a basis for the human Nav1.7 Inherited Erythromelalgia S241T mutation Deposited 2022-06-29 | Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain A
1–239（239 aa）
|
Mutation：V126T | PX4 1,2-DIMYRISTOYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 20 CPS 3-[(3-CHOLAMIDOPROPYL)DIMETHYLAMMONIO]-1-PROPANESULFONATE × 8 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5.2；277 K；1.8 M Ammonium Sulfate
0.1 M Sodium Citrate pH 5.2
|
Resolution 3.10 Å R-free 0.260 |
| 8H9O Crystal structure of voltage-gated sodium channel NavAb N49K mutant in sodium ion condition Deposited 2022-10-25 | Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain A
1–267（267 aa）
|
Mutation：N49K | LMT DODECYL-BETA-D-MALTOSIDE × 8 1N7 CHAPSO × 4 PX4 1,2-DIMYRISTOYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 36 NA SODIUM ION × 4 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；293 K；9%-11% PEG MME 2000, 100 mM sodium chloride, 100 mM magnesium nitrate, 25 mM cadmium nitrate, 100 mM Tris-HCl, pH 8.4
|
Resolution 3.30 Å R-free 0.272 |
| 8H9W Crystal structure of voltage-gated sodium channel NavAb N49K mutant in calcium ion condition Deposited 2022-10-25 | Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain A
1–267（267 aa）
|
Mutation：N49K | LMT DODECYL-BETA-D-MALTOSIDE × 8 1N7 CHAPSO × 4 PX4 1,2-DIMYRISTOYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 32 CA CALCIUM ION × 4 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；9%-11% PEG MME 2000, 100 mM sodium chloride, 100 mM magnesium nitrate, 25 mM cadmium nitrate, 100 mM Tris-HCl, pH 8.4
|
Resolution 2.70 Å R-free 0.264 |
| 8H9X Crystal structure of voltage-gated sodium channel NavAb N49K/L176Q mutant in sodium ion condition Deposited 2022-10-25 | Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain A
1–267（267 aa）
|
Mutation：N49K, L176Q | LMT DODECYL-BETA-D-MALTOSIDE × 8 1N7 CHAPSO × 4 PX4 1,2-DIMYRISTOYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 32 NA SODIUM ION × 8 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；9%-11% PEG MME 2000, 100 mM sodium chloride, 100 mM magnesium nitrate, 25 mM cadmium nitrate, 100 mM Tris-HCl, pH 8.4
|
Resolution 3.40 Å R-free 0.280 |
| 8H9Y Crystal structure of voltage-gated sodium channel NavAb N49K/L176Q mutant in calcium ion condition Deposited 2022-10-25 | Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain A
1–267（267 aa）
|
Mutation：N49K, L176Q | LMT DODECYL-BETA-D-MALTOSIDE × 8 1N7 CHAPSO × 4 PX4 1,2-DIMYRISTOYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 32 CA CALCIUM ION × 8 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；9%-11% PEG MME 2000, 100 mM sodium chloride, 100 mM magnesium nitrate, 25 mM cadmium nitrate, 100 mM Tris-HCl, pH 8.4
|
Resolution 3.40 Å R-free 0.264 |
| 8HA1 Crystal structure of voltage-gated sodium channel NavAb N49K/L176G mutant in sodium ion condition Deposited 2022-10-26 | Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain A
1–267（267 aa）
|
Mutation：N49K, L176G | LMT DODECYL-BETA-D-MALTOSIDE × 8 1N7 CHAPSO × 4 PX4 1,2-DIMYRISTOYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 32 NA SODIUM ION × 8 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；9%-11% PEG MME 2000, 100 mM sodium chloride, 100 mM magnesium nitrate, 25 mM cadmium nitrate, 100 mM Tris-HCl, pH 8.4
|
Resolution 3.50 Å R-free 0.297 |
| 8HA2 Crystal structure of voltage-gated sodium channel NavAb N49K/L176G mutant in calcium ion condition Deposited 2022-10-26 | Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain A
1–267（267 aa）
|
Mutation：N49K, L176G | LMT DODECYL-BETA-D-MALTOSIDE × 8 1N7 CHAPSO × 4 PX4 1,2-DIMYRISTOYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 32 CA CALCIUM ION × 8 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；9%-11% PEG MME 2000, 100 mM sodium chloride, 100 mM magnesium nitrate, 25mM cadmium nitrate, 100 mM Tris-HCl, pH 8.4
|
Resolution 3.30 Å R-free 0.281 |
| 9UC1 Crystal structure of voltage-gated sodium channel NavAb N49K/S178T mutant Deposited 2025-04-03 | Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain A
1–267（267 aa）
|
Mutation：N49K, S178T | CA CALCIUM ION × 12 1N7 CHAPSO × 4 LMT DODECYL-BETA-D-MALTOSIDE × 4 PX4 1,2-DIMYRISTOYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 32 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8.4；293 K；9%-11% PEG MME 2000, 100mM sodium chloride, 100mM magnesium nitrate, 25mM cadmium nitrate, 100mM Tris-HCl, pH 8.4
|
Resolution 2.50 Å R-free 0.274 |
| 9UC2 Crystal structure of voltage-gated sodium channel NavAb N49K/S178A mutant Deposited 2025-04-03 | Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain A
1–267（267 aa）
|
Mutation：N49K,S178A | CA CALCIUM ION × 12 1N7 CHAPSO × 4 LMT DODECYL-BETA-D-MALTOSIDE × 4 PX4 1,2-DIMYRISTOYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 32 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8.4；293 K；9%-11% PEG MME 2000, 100mM sodium chloride, 100mM magnesium nitrate, 25mM cadmium nitrate, 100mM Tris-HCl, pH 8.4
|
Resolution 2.80 Å R-free 0.294 |
| 9UC3 Crystal structure of voltage-gated sodium channel NavAb N49K/S178G mutant Deposited 2025-04-03 | Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain A
1–267（267 aa）
|
Mutation：N49K,S178G | CA CALCIUM ION × 12 1N7 CHAPSO × 4 LMT DODECYL-BETA-D-MALTOSIDE × 4 PX4 1,2-DIMYRISTOYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 32 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8.4；293 K；9%-11% PEG MME 2000, 100mM sodium chloride, 100mM magnesium nitrate, 25mM cadmium nitrate, 100mM Tris-HCl, pH 8.4
|
Resolution 2.90 Å R-free 0.299 |
| 9UC4 Crystal structure of voltage-gated sodium channel NavAb N49K/S178T/T206A mutant Deposited 2025-04-03 | Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain A
1–267（267 aa）
|
Mutation：N49K, S178T, T206A | CA CALCIUM ION × 12 1N7 CHAPSO × 4 LMT DODECYL-BETA-D-MALTOSIDE × 4 PX4 1,2-DIMYRISTOYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 32 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；9%-11% PEG MME 2000, 100mM sodium chloride, 100mM magnesium nitrate, 25mM cadmium nitrate, 100mM Tris-HCl, pH 8.4
|
Resolution 3.40 Å R-free 0.312 |
| 9VDQ Crystal structure of voltage-gated sodium channel NavAb N49K mutant Deposited 2025-06-09 | Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain A
1–267（267 aa）
|
Mutation：N49K | NA SODIUM ION × 12 1N7 CHAPSO × 4 PX4 1,2-DIMYRISTOYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 32 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；9%-11% PEG MME 2000, 100mM sodium chloride, 100mM magnesium nitrate, 25mM cadmium nitrate, 100mM Tris-HCl, pH 8.4
|
Resolution 3.10 Å R-free 0.289 |
| 9VDR Crystal structure of voltage-gated sodium channel NavAb N49K/L176F mutant Deposited 2025-06-09 | Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain A
1–267（267 aa）
|
Mutation：N49K/L176F | NA SODIUM ION × 8 1N7 CHAPSO × 4 LMT DODECYL-BETA-D-MALTOSIDE × 4 PX4 1,2-DIMYRISTOYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 12 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；9%-11% PEG MME 2000, 100 mM sodium chloride, 100mM magnesium nitrate, 25mM cadmium nitrate, 100mM Tris-HCl, pH 8.4
|
Resolution 3.70 Å R-free 0.327 |
| 9VDS Crystal structure of voltage-gated sodium channel NavAb N49K/L176W mutant Deposited 2025-06-09 | Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain A
1–267（267 aa）
|
Mutation：N49K/L176W | NA SODIUM ION × 4 1N7 CHAPSO × 4 LMT DODECYL-BETA-D-MALTOSIDE × 4 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；9%-11% PEG MME 2000, 100mM sodium chloride, 100mM magnesium nitrate, 25mM cadmium nitrate, 100mM Tris-HCl, pH 8.4
|
Resolution 3.70 Å R-free 0.339 |
| 9VDT Crystal structure of voltage-gated sodium channel NavAb N49K DC230 mutant Deposited 2025-06-09 | Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain A
1–267（267 aa）
|
Mutation：N49K | NA SODIUM ION × 8 1N7 CHAPSO × 4 LMT DODECYL-BETA-D-MALTOSIDE × 4 PX4 1,2-DIMYRISTOYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 32 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；7%-9% PEG 6000, 100mM sodium chloride, 100mM magnesium nitrate, 100mM cadmium chloride, 10mM copper chloride, 100mM Tris-HCl, pH 8.4
|
Resolution 3.50 Å R-free 0.344 |
| 9VDU Crystal structure of voltage-gated sodium channel NavAb N49K/L176F DC230 mutant Deposited 2025-06-09 | Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain A
1–230（230 aa）
|
Mutation：N49K/L176F | NA SODIUM ION × 8 1N7 CHAPSO × 4 PX4 1,2-DIMYRISTOYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 28 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；7%-9% PEG 6000, 100mM sodium chloride, 100mM magnesium nitrate, 100mM cadmium chloride, 10mM copper chloride, 100mM Tris-HCl, pH 8.4
|
Resolution 3.40 Å R-free 0.304 |