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Protein Structures and Oligomeric States

Enter a protein name, UniProt accession or PDB ID. Name searches ask you to confirm the protein identity before related structures are queried.

Current Protein Identity：C3SR17 New Search
69PDB entries
69Biological assembly
69chain/assembly records
Main Difference Dimensions in This Set
Different construct Different assembly state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics

Difference tags compare only the current result set; every original PDB and assembly record remains separate.

Related-Structure Differences

Each row represents one biological assembly in one PDB entry; multiple monomers of the same protein are listed separately.

PDB Entry Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Experimental Method Experimental Conditions Structure Quality
4V85 Crystal Structure of Release Factor RF3 Trapped in the GTP State on a Rotated Conformation of the Ribosome. Deposited 2011-06-13 Assembly 1 Protein–RNA Heteromer；Protein × 55 PDB declaration: 59-meric（59） Consistent with all polymers
Chain BG 1–177（177 aa）
Not recorded MG MAGNESIUM ION × 493 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；295 K；Tris Ac PH.7.0, 25-35 mM KCL, 6.1% PEG 20000, 1% glycerol, 50mM sucrose, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 3.20 Å R-free 0.250
7YLA Cryo-EM structure of 50S-HflX complex Deposited 2022-07-25 Assembly 1 Protein–RNA Heteromer；Protein × 30 PDB declaration: 32-meric（32） Consistent with all polymers
Chain O 2–177（176 aa）
Not recorded GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 3 NA SODIUM ION × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.52 Å
8AKN Cryo-EM structure of the proline-rich antimicrobial peptide drosocin bound to the terminating ribosome Deposited 2022-07-30 Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 57-meric（57） Consistent with all polymers
Chain g 1–177（177 aa）
Not recorded ZN ZINC ION × 2 A2G 2-acetamido-2-deoxy-alpha-D-galactopyranose × 1 MG MAGNESIUM ION × 305 SPM SPERMINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 2.30 Å
8AM9 Cryo-EM structure of the proline-rich antimicrobial peptide drosocin bound to the elongating ribosome Deposited 2022-08-03 Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric（56） Consistent with all polymers
Chain g 1–177（177 aa）
Not recorded ZN ZINC ION × 2 MG MAGNESIUM ION × 293 SPM SPERMINE × 1 A2G 2-acetamido-2-deoxy-alpha-D-galactopyranose × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 2.80 Å
8ANA Cryo-EM structure of the proline-rich antimicrobial peptide drosocin bound to the 50S ribosomal subunit Deposited 2022-08-05 Assembly 1 Protein–RNA Heteromer；Protein × 30 PDB declaration: 32-meric（32） Consistent with all polymers
Chain g 1–177（177 aa）
Not recorded ZN ZINC ION × 2 A2G 2-acetamido-2-deoxy-alpha-D-galactopyranose × 1 MG MAGNESIUM ION × 216 SPM SPERMINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 2.10 Å
8EKC Escherichia coli 70S ribosome bound to thermorubin, deacylated P-site tRNAfMet and aminoacylated A-site Phe-tRNA Deposited 2022-09-20 Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric（55） Consistent with all polymers
Chain G 1–177（177 aa）
Not recorded MG MAGNESIUM ION × 714 T8B Thermorubin × 1 PHE PHENYLALANINE × 1 ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.70 Å
8FZD Cryo-EM structure of an E. coli non-rotated ribosome termination complex bound with apoRF3, RF1, P- and E-site tRNAPhe (Composite state I-B) Deposited 2023-01-28 Assembly 1 Protein–RNA Heteromer；Protein × 53 PDB declaration: 59-meric（59） Consistent with all polymers
Chain G 1–177（177 aa）
Not recorded MG MAGNESIUM ION × 581 ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；5 mM Tris-HCl, 60 mM NH4Cl, 10 mM MgCl2, 6 mM B-mercaptoethanol
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
8FZE Cryo-EM structure of an E. coli non-rotated ribosome termination complex bound with RF1, P- and E-site tRNAPhe (State I-A) Deposited 2023-01-28 Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 58-meric（58） Consistent with all polymers
Chain G 1–177（177 aa）
Not recorded MG MAGNESIUM ION × 570 ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；5 mM Tris-HCl, 60 mM NH4Cl, 10 mM MgCl2, 6 mM B-mercaptoethanol
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
8FZF Cryo-EM structure of an E. coli rotated ribosome complex bound with RF3-ppGpp and p/E-tRNAPhe (Composite state I-C) Deposited 2023-01-28 Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 56-meric（56） Consistent with all polymers
Chain G 1–177（177 aa）
Not recorded MG MAGNESIUM ION × 384 G4P GUANOSINE-5',3'-TETRAPHOSPHATE × 1 ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；5 mM Tris-HCl, 60 mM NH4Cl, 10 mM MgCl2, 6 mM B-mercaptoethanol
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
8FZG Cryo-EM structure of an E. coli non-rotated ribosome termination complex bound with RF3-GDPCP, RF1, P- and E-site tRNAPhe (Composite state II-A) Deposited 2023-01-28 Assembly 1 Protein–RNA Heteromer；Protein × 53 PDB declaration: 59-meric（59） Consistent with all polymers
Chain G 1–177（177 aa）
Not recorded MG MAGNESIUM ION × 428 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；5 mM Tris-HCl, 60 mM NH4Cl, 10 mM MgCl2, 6 mM B-mercaptoethanol
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
8FZH Cryo-EM structure of an E. coli non-rotated ribosome termination complex bound with RF1, P- and E-site tRNAPhe (State II-D) Deposited 2023-01-28 Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 58-meric（58） Consistent with all polymers
Chain G 1–177（177 aa）
Not recorded MG MAGNESIUM ION × 595 ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；5 mM Tris-HCl, 60 mM NH4Cl, 10 mM MgCl2, 6 mM B-mercaptoethanol
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
8FZI Cryo-EM structure of an E. coli rotated ribosome bound with RF3-GDPCP and p/E-tRNAPhe (Composite state II-B) Deposited 2023-01-28 Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 56-meric（56） Consistent with all polymers
Chain G 1–177（177 aa）
Not recorded MG MAGNESIUM ION × 737 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；5 mM Tris-HCl, 60 mM NH4Cl, 10 mM MgCl2, 6 mM B-mercaptoethanol
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
8FZJ Cryo-EM structure of an E. coli rotated ribosome bound with RF3-GDPCP and p/E-tRNAPhe (Composite state II-C) Deposited 2023-01-28 Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 56-meric（56） Consistent with all polymers
Chain G 1–177（177 aa）
Not recorded MG MAGNESIUM ION × 757 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；5 mM Tris-HCl, 60 mM NH4Cl, 10 mM MgCl2, 6 mM B-mercaptoethanol
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
8G7P Structure of the Escherichia coli 70S ribosome in complex with EF-Tu and Ile-tRNAIle(LAU) bound to the cognate AUA codon (Structure I) Deposited 2023-02-16 Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 58-meric（58） Consistent with all polymers
Chain G 1–177（177 aa）
Not recorded MG MAGNESIUM ION × 876 K POTASSIUM ION × 2 ILE ISOLEUCINE × 2 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
8G7Q Structure of the Escherichia coli 70S ribosome in complex with EF-Tu and Ile-tRNAIle(LAU) bound to the near-cognate AUG codon (Structure II) Deposited 2023-02-16 Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 58-meric（58） Consistent with all polymers
Chain G 1–177（177 aa）
Not recorded PAR PAROMOMYCIN × 1 MG MAGNESIUM ION × 950 ILE ISOLEUCINE × 2 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
8G7R Structure of the Escherichia coli 70S ribosome in complex with A-site tRNAIle(LAU) bound to the cognate AUA codon (Structure III) Deposited 2023-02-16 Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric（56） Consistent with all polymers
Chain G 1–177（177 aa）
Not recorded PAR PAROMOMYCIN × 1 MG MAGNESIUM ION × 734 K POTASSIUM ION × 2 ILE ISOLEUCINE × 1 ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.80 Å
8G7S Structure of the Escherichia coli 70S ribosome in complex with P-site tRNAIle(LAU) bound to the cognate AUA codon (Structure IV) Deposited 2023-02-16 Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric（55） Consistent with all polymers
Chain G 1–177（177 aa）
Not recorded MG MAGNESIUM ION × 564 ILE ISOLEUCINE × 1 ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
8SYL Cryo-EM structure of the Escherichia coli 70S ribosome in complex with amikacin, mRNA, and A-, P-, and E-site tRNAs Deposited 2023-05-25 Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric（56） Consistent with all polymers
Chain G 1–177（177 aa）
Not recorded MG MAGNESIUM ION × 474 AKN (2S)-N-[(1R,2S,3S,4R,5S)-4-[(2R,3R,4S,5S,6R)-6-(aminomethyl)-3,4,5-tris(oxidanyl)oxan-2-yl]oxy-5-azanyl-2-[(2S,3R,4S,5S ,6R)-4-azanyl-6-(hydroxymethyl)-3,5-bis(oxidanyl)oxan-2-yl]oxy-3-oxidanyl-cyclohexyl]-4-azanyl-2-oxidanyl-butanamide × 2 PHE PHENYLALANINE × 1 ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
8UPO Escherichia coli transcription-translation coupled complex class A (TTC-A) containing RfaH bound to ops signal, mRNA with a 21 nt long spacer, and fMet-tRNAs in E-site and P-site of the ribosome Deposited 2023-10-23 Assembly 1 Other combination Heteromer；Protein × 58 PDB declaration: 66-meric（66） Consistent with all polymers
Chain p 1–177（177 aa）
Not recorded MG MAGNESIUM ION × 1 ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 5.50 Å
8UPR Escherichia coli transcription-translation coupled complex class A (TTC-A) containing RfaH bound to ops signal, mRNA with a 21 nt long spacer, and fMet-tRNAs in E-site and P-site of the ribosome Deposited 2023-10-23 Assembly 1 Other combination Heteromer；Protein × 59 PDB declaration: 67-meric（67） Consistent with all polymers
Chain p 1–177（177 aa）
Not recorded MG MAGNESIUM ION × 1 ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 5.30 Å
8UQL Escherichia coli transcription-translation coupled complex class B (TTC-B) containing RfaH in loaded state, mRNA with a 24 nt long spacer, and fMet-tRNAs in E-site and P-site of the ribosome Deposited 2023-10-24 Assembly 1 Other combination Heteromer；Protein × 59 PDB declaration: 67-meric（67） Consistent with all polymers
Chain p 1–177（177 aa）
Not recorded MG MAGNESIUM ION × 1 ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
8UQM Escherichia coli transcription-translation coupled complex class B (TTC-B) containing RfaH in loaded state, NusA, mRNA with a 24 nt long spacer, and fMet-tRNAs in E-site and P-site of the ribosome Deposited 2023-10-24 Assembly 1 Other combination Heteromer；Protein × 60 PDB declaration: 68-meric（68） Consistent with all polymers
Chain p 1–177（177 aa）
Not recorded MG MAGNESIUM ION × 1 ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 5.30 Å
8UQP Escherichia coli transcription-translation coupled complex class B (TTC-B) containing RfaH bound to ops signal, mRNA with a 24 nt long spacer, and fMet-tRNAs in E-site and P-site of the ribosome Deposited 2023-10-24 Assembly 1 Other combination Heteromer；Protein × 59 PDB declaration: 67-meric（67） Consistent with all polymers
Chain p 1–177（177 aa）
Not recorded MG MAGNESIUM ION × 1 ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.80 Å
8UR0 Escherichia coli transcription-translation coupled complex class B (TTC-B) containing RfaH bound to ops signal, NusA, mRNA with a 24 nt long spacer, and fMet-tRNAs in E-site and P-site of the ribosome Deposited 2023-10-25 Assembly 1 Other combination Heteromer；Protein × 60 PDB declaration: 68-meric（68） Consistent with all polymers
Chain p 1–177（177 aa）
Not recorded MG MAGNESIUM ION × 1 ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
8URH Escherichia coli transcription-translation coupled complex class B (TTC-B) containing RfaH bound to ops signal, mRNA with a 27 nt long spacer, and fMet-tRNAs in E-site and P-site of the ribosome Deposited 2023-10-26 Assembly 1 Other combination Heteromer；Protein × 59 PDB declaration: 67-meric（67） Consistent with all polymers
Chain p 1–177（177 aa）
Not recorded MG MAGNESIUM ION × 1 ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 5.70 Å
8URI Escherichia coli transcription-translation coupled complex class B (TTC-B) containing RfaH bound to ops signal, NusA, mRNA with a 27 nt long spacer, and fMet-tRNAs in E-site and P-site of the ribosome Deposited 2023-10-26 Assembly 1 Other combination Heteromer；Protein × 60 PDB declaration: 68-meric（68） Consistent with all polymers
Chain p 1–177（177 aa）
Not recorded MG MAGNESIUM ION × 1 ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 5.30 Å
8URM E. coli 70S ribosome with unmodified tRNAPro(GGG) bound to slippery P-site CCC-C codon and tRNAVal(UAC) in the A site Deposited 2023-10-26 Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric（55） Consistent with all polymers
Chain F 1–177（177 aa）
Not recorded MG MAGNESIUM ION × 395 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
8URX Escherichia coli transcription-translation coupled complex class B (TTC-B) containing RfaH bound to ops signal, mRNA with a 30 nt long spacer, and fMet-tRNAs in E-site and P-site of the ribosome Deposited 2023-10-27 Assembly 1 Other combination Heteromer；Protein × 59 PDB declaration: 67-meric（67） Consistent with all polymers
Chain p 1–177（177 aa）
Not recorded MG MAGNESIUM ION × 1 ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 6.60 Å
8URY Escherichia coli transcription-translation coupled complex class B (TTC-B) containing RfaH bound to ops signal, NusA, mRNA with a 30 nt long spacer, and fMet-tRNA in E-site and P-site of the ribosome Deposited 2023-10-27 Assembly 1 Other combination Heteromer；Protein × 60 PDB declaration: 68-meric（68） Consistent with all polymers
Chain p 1–177（177 aa）
Not recorded MG MAGNESIUM ION × 1 ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
8UTJ E. coli 70S ribosome with unmodified lys-tRNAPro(GGG) bound to slippery P-site CCC-C codon in the 0 frame Deposited 2023-10-31 Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric（55） Consistent with all polymers
Chain F 1–177（177 aa）
Not recorded MG MAGNESIUM ION × 530 LYS LYSINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.50 Å
8UX8 E. coli 70S ribosome with unmodified Lys-tRNAPro(GGG) bound to slippery P-site CCC-C codon in the +1 mRNA reading frame Deposited 2023-11-09 Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric（55） Consistent with all polymers
Chain F 1–177（177 aa）
Not recorded MG MAGNESIUM ION × 395 LYS LYSINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
8UXB E. coli 70S ribosome with unmodified P/E-tRNAPro(GGG) bound to slippery P-site CCC-C codon Deposited 2023-11-09 Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 53-meric（53） Consistent with all polymers
Chain F 1–177（177 aa）
Not recorded MG MAGNESIUM ION × 287 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
8UZ3 E. coli 70S ribosome with unmodified e*/E-tRNAPro(GGG) bound to slippery P-site CCC-C codon Deposited 2023-11-14 Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 53-meric（53） Consistent with all polymers
Chain F 1–177（177 aa）
Not recorded MG MAGNESIUM ION × 415 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
8UZG E. coli 70S ribosome with unmodified e*/E-tRNAPro(GGG) bound to slippery P-site CCC-C codon Deposited 2023-11-15 Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 53-meric（53） Consistent with all polymers
Chain F 1–177（177 aa）
Not recorded MG MAGNESIUM ION × 398 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.70 Å
8V03 E. coli 70S ribosome with unmodified P/E-tRNAPro(GGG) bound to slippery P-site CCC-C codon Deposited 2023-11-16 Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 53-meric（53） Consistent with all polymers
Chain F 1–177（177 aa）
Not recorded MG MAGNESIUM ION × 281 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.00 Å
8VKV Escherichia coli transcription-translation coupled complex (TTC-B) containing mRNA with a 33 nt long spacer, ops signal, RfaH, NusA, and fMet-tRNAs in E-site and P-site Deposited 2024-01-10 Assembly 1 Other combination Heteromer；Protein × 60 PDB declaration: 68-meric（68） Consistent with all polymers
Chain p 1–177（177 aa）
Not recorded MG MAGNESIUM ION × 1 ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
8VL1 Escherichia coli transcription-translation loosely coupled complex (TTC-LC) containing mRNA with a 36 nt long spacer, ops signal, RfaH, NusA, and fMet-tRNAs in E-site and P-site Deposited 2024-01-11 Assembly 1 Other combination Heteromer；Protein × 60 PDB declaration: 68-meric（68） Consistent with all polymers
Chain p 1–177（177 aa）
Not recorded MG MAGNESIUM ION × 1 ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 5.30 Å
8VOO Escherichia coli transcription-translation loosely coupled complex (TTC-LC) containing mRNA with a 39 nt long spacer, ops signal, RfaH, NusA, and fMet-tRNAs in E-site and P-site Deposited 2024-01-15 Assembly 1 Other combination Heteromer；Protein × 60 PDB declaration: 68-meric（68） Consistent with all polymers
Chain p 1–177（177 aa）
Not recorded MG MAGNESIUM ION × 1 ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
8VOP Escherichia coli transcription-translation coupled complex (TTC-B) containing mRNA with a 36 nt long spacer, NusG, NusA, and fMet-tRNAs in E-site and P-site Deposited 2024-01-15 Assembly 1 Other combination Heteromer；Protein × 60 PDB declaration: 68-meric（68） Consistent with all polymers
Chain p 1–177（177 aa）
Not recorded MG MAGNESIUM ION × 1 ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 6.00 Å
8VOQ Escherichia coli transcription-translation loosely coupled complex (TTC-LC) containing mRNA with a 39 nt long spacer, NusG, NusA, and fMet-tRNAs in E-site and P-site Deposited 2024-01-15 Assembly 1 Other combination Heteromer；Protein × 60 PDB declaration: 68-meric（68） Consistent with all polymers
Chain p 1–177（177 aa）
Not recorded MG MAGNESIUM ION × 1 ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 9.30 Å
8VOR Escherichia coli transcription-translation loosely coupled complex (TTC-LC) containing mRNA with a 51 nt long spacer, NusG, NusA, and fMet-tRNAs in E-site and P-site Deposited 2024-01-15 Assembly 1 Other combination Heteromer；Protein × 60 PDB declaration: 68-meric（68） Consistent with all polymers
Chain p 1–177（177 aa）
Not recorded MG MAGNESIUM ION × 1 ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
8VS9 Endogenous trans-translation complex with tmRNA*SmpB in the P site and alanyl-tRNA in the A site and deacyl-tRNA in the E site of E. coli 70S ribosome Deposited 2024-01-23 Assembly 1 Protein–RNA Heteromer；Protein × 53 PDB declaration: 59-meric（59） Consistent with all polymers
Chain L06 1–177（177 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.90 Å
8VSA Endogenous trans-translation complex with tmRNA*SmpB in the P site and alanyl-tRNA in the A site of E. coli 70S ribosome Deposited 2024-01-23 Assembly 1 Protein–RNA Heteromer；Protein × 53 PDB declaration: 58-meric（58） Consistent with all polymers
Chain L06 1–177（177 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.70 Å
8VUP E. coli 70S ribosome with unmodified tRNAPro(GGG) in the e*/E conformation on a slippery CCC-C codon Deposited 2024-01-29 Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 53-meric（53） Consistent with all polymers
Chain F 2–177（176 aa）
Not recorded MG MAGNESIUM ION × 239 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
8VV4 E. coli 70S ribosome with unmodified Lys-tRNAPro(GGG) in the P/P conformation on a slippery CCC-C codon Deposited 2024-01-30 Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 54-meric（54） Consistent with all polymers
Chain F 1–177（177 aa）
Not recorded MG MAGNESIUM ION × 424 LYS LYSINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
8VWQ E. coli 70S ribosome with unmodified Lys-tRNAPro(GGG) in the P/P conformation on a slippery CCC-C codon and Elongation Factor P bound (uL1 in the closed conformation) Deposited 2024-02-02 Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 56-meric（56） Consistent with all polymers
Chain F 1–177（177 aa）
Not recorded MG MAGNESIUM ION × 586 LYS LYSINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
8W2N E. coli 70S ribosome with unmodified Lys-tRNAPro(GGG) in the P/P conformation on a slippery CCC-C codon and Elongation Factor P bound (uL1 in the open conformation) Deposited 2024-02-20 Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric（55） Consistent with all polymers
Chain F 1–177（177 aa）
Not recorded MG MAGNESIUM ION × 481 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
9AX7 70S initiation complex (tRNA-fMet M1 + CUG start codon) Deposited 2024-03-06 Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 54-meric（54） Consistent with all polymers
Chain g 1–177（177 aa）
Not recorded ZN ZINC ION × 2 MG MAGNESIUM ION × 309 IAS BETA-L-ASPARTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.63 Å
9AX8 70S initiation complex (tRNA-fMet M1, initiation factor 2 + CUG start codon) Deposited 2024-03-06 Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 53-meric（53） Consistent with all polymers
Chain H 2–177（176 aa）
Not recorded MG MAGNESIUM ION × 287 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.60 Å
9B4Z E. coli 70S ribosome complex (N1-methylated 16S A1408 + arbekacin) Deposited 2024-03-21 Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric（56） Consistent with all polymers
Chain BG 1–177（177 aa）
Not recorded MG MAGNESIUM ION × 309 84G Arbekacin × 2 ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.20 Å
9B50 E. coli 70S ribosome complex (unmethylated 16S A1408 + arbekacin) Deposited 2024-03-21 Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 52-meric（52） Consistent with all polymers
Chain BG 1–177（177 aa）
Not recorded MG MAGNESIUM ION × 309 84G Arbekacin × 2 ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.70 Å
9B51 E. coli 70S ribosome complex (N1-methylated 16S A1408 + G418) Deposited 2024-03-21 Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric（56） Consistent with all polymers
Chain BG 1–177（177 aa）
Not recorded MG MAGNESIUM ION × 309 GET GENETICIN × 2 ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.40 Å
9CG5 70S initiation complex (tRNA-fMet M1 + UUG start codon) Deposited 2024-06-28 Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 54-meric（54） Consistent with all polymers
Chain g 1–177（177 aa）
Not recorded ZN ZINC ION × 2 MG MAGNESIUM ION × 309 IAS BETA-L-ASPARTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.59 Å
9CG6 70S initiation complex (tRNA-fMet M1 + GUG start codon) Deposited 2024-06-28 Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 54-meric（54） Consistent with all polymers
Chain g 1–177（177 aa）
Not recorded ZN ZINC ION × 2 MG MAGNESIUM ION × 309 IAS BETA-L-ASPARTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.61 Å
9CG7 70S initiation complex (tRNA-fMet M1 + AUG start codon) Deposited 2024-06-28 Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 54-meric（54） Consistent with all polymers
Chain g 1–177（177 aa）
Not recorded ZN ZINC ION × 2 MG MAGNESIUM ION × 309 IAS BETA-L-ASPARTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.75 Å
9D89 E. coli 50S ribosomal subunit in complex with PrAMP rumicidin-2 (focused refinement) Deposited 2024-08-19 Assembly 1 Protein–RNA Heteromer；Protein × 29 PDB declaration: 31-meric（31） Consistent with all polymers
Chain g 2–177（176 aa）
Not recorded ZN ZINC ION × 1 MG MAGNESIUM ION × 204 K POTASSIUM ION × 93 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 1.95 Å
9NJF E. coli pre-elongation complex without an A-site tRNA with EQ2-YbiT in Non-hydrolytic 1/PtIM(a) conformation Deposited 2025-02-27 Assembly 1 Protein–RNA Heteromer；Protein × 53 PDB declaration: 58-meric（58） Consistent with all polymers
Chain 6 2–177（176 aa）
Not recorded MG MAGNESIUM ION × 124 ZN ZINC ION × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 NA SODIUM ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
9NJV E. coli 70S initiation complex (bL33 absent) Deposited 2025-02-28 Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 53-meric（53） Consistent with all polymers
Chain 6 2–177（176 aa）
Not recorded MG MAGNESIUM ION × 152 FME N-FORMYLMETHIONINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
9NKL E. coli 70S initiation complex Deposited 2025-03-01 Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 54-meric（54） Consistent with all polymers
Chain 6 2–177（176 aa）
Not recorded MG MAGNESIUM ION × 161 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
9NL5 E. coli pre-elongation complex without an A-site tRNA with EQ2-EttA in Hydrolytic 1 conformation Deposited 2025-03-02 Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 56-meric（56） Consistent with all polymers
Chain 6 2–177（176 aa）
Not recorded MG MAGNESIUM ION × 125 ZN ZINC ION × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 NA SODIUM ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
9NL6 E. coli initiation complex with EQ2-YbiT in Non-hydrolytic 1/PtIM(a) conformation Deposited 2025-03-02 Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric（55） Consistent with all polymers
Chain 6 2–177（176 aa）
Not recorded MG MAGNESIUM ION × 252 FME N-FORMYLMETHIONINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 NA SODIUM ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
9NL7 E. coli initiation complex with EQ2-YbiT in Non-hydrolytic 2/PtIM(b) conformation Deposited 2025-03-02 Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 56-meric（56） Consistent with all polymers
Chain 6 2–177（176 aa）
Not recorded MG MAGNESIUM ION × 250 FME N-FORMYLMETHIONINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 NA SODIUM ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
9NLB E. coli initiation complex with EQ2-EttA in Hydrolytic 2 conformation Deposited 2025-03-03 Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 56-meric（56） Consistent with all polymers
Chain 6 2–177（176 aa）
Not recorded MG MAGNESIUM ION × 279 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 NA SODIUM ION × 2 FME N-FORMYLMETHIONINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
9NLE E. coli initiation complex with EQ2-EttA in Hydrolytic 1 conformation Deposited 2025-03-03 Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 56-meric（56） Consistent with all polymers
Chain 6 2–177（176 aa）
Not recorded MG MAGNESIUM ION × 298 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 NA SODIUM ION × 2 FME N-FORMYLMETHIONINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
9NLF E. coli initiation complex with EQ2-YbiT in Hydrolytic 2/PtIM(a) conformation Deposited 2025-03-03 Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 56-meric（56） Consistent with all polymers
Chain 6 2–177（176 aa）
Not recorded MG MAGNESIUM ION × 245 FME N-FORMYLMETHIONINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 NA SODIUM ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
9NLH E.coli Initiation complex with YheS-EQ2 Deposited 2025-03-03 Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric（55） Consistent with all polymers
Chain 6 2–177（176 aa）
Not recorded MG MAGNESIUM ION × 263 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 NA SODIUM ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
9NLJ E.coli Initiation complex with Uup-EQ2 Deposited 2025-03-03 Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 56-meric（56） Consistent with all polymers
Chain 6 2–177（176 aa）
Not recorded MG MAGNESIUM ION × 277 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 NA SODIUM ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
9NLQ E. coli initiation complex with EQ2-YbiT in Hydrolytic 1/PtIM(a) conformation Deposited 2025-03-03 Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 56-meric（56） Consistent with all polymers
Chain 6 2–177（176 aa）
Not recorded MG MAGNESIUM ION × 241 FME N-FORMYLMETHIONINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 NA SODIUM ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
9NLS E. coli initiation complex with EQ2-YbiT in Intermediate/PtIM(b) conformation Deposited 2025-03-03 Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 56-meric（56） Consistent with all polymers
Chain 6 2–177（176 aa）
Not recorded MG MAGNESIUM ION × 248 ZN ZINC ION × 1 FME N-FORMYLMETHIONINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 NA SODIUM ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å

This page presents structural evidence recorded in PDB/mmCIF.“No recorded non-water small molecule” does not prove that the experimental sample was ligand-free; experimental conditions belong to the PDB experiment, while ligands and copy counts are shown for each biological assembly.