8ana

Cryo-EM structure of the proline-rich antimicrobial peptide drosocin bound to the 50S ribosomal subunit

Method: ELECTRON MICROSCOPY D_max: 226.5 Å Quality: GOOD

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1. Protein Identity and Related Structures Protein Identity & Related Structures

50S ribosomal protein L33

OrganismNot specified

State in the Current Structure

Assembly	Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Associated Components	Method and Experimental Conditions	Structure Quality
1	Protein–RNA Heteromer Protein × 30 RNA 2 PDB declaration: 32-meric（32） Consistent with all polymer counts	Chain 0； UniProt 1–55	Not recorded	50S ribosomal protein L34 × 1 （A0A8X6EVB8） 50S ribosomal protein L35 × 1 （A0A8S0FT45） 50S ribosomal protein L36 × 1 （A0A2X1PTA3） 50S ribosomal protein L31 × 1 （C3SIV2） Drosocin1 × 1 （B4MNS1） 23S ribosomal RNA × 1 5S ribosomal RNA × 1 50S ribosomal protein L2 × 1 （A0A7L6YWH1） 50S ribosomal protein L3 × 1 （C3SQU2） 50S ribosomal protein L4 × 1 （D8A1N0） 50S ribosomal protein L5 × 1 （D6IEK6） 50S ribosomal protein L6 × 1 （C3SR17） 50S ribosomal protein L9 × 1 （C3SFP2） 50S ribosomal protein L13 × 1 （A0A656YGQ7） 50S ribosomal protein L14 × 1 （C3SQZ2） 50S ribosomal protein L15 × 1 （P02413） 50S ribosomal protein L16 × 1 （A0A0H3EMH6） 50S ribosomal protein L17 × 1 （A0A1X3JBW6） 50S ribosomal protein L18 × 1 （A0A514EKV0） 50S ribosomal protein L19 × 1 （A0A370V6W8） 50S ribosomal protein L20 × 1 （A0A378BCJ4） 50S ribosomal protein L21 × 1 （A0A0E2L2H1） 50S ribosomal protein L22 × 1 （A0A0F6B7N1） 50S ribosomal protein L23 × 1 （D7Y3D8） 50S ribosomal protein L24 × 1 （S1E9P3） 50S ribosomal protein L25 × 1 （P68919） 50S ribosomal protein L27 × 1 （C3SSG7） 50S ribosomal protein L28 × 1 （C3SME2） 50S ribosomal protein L29 × 1 （P0A7M6） 50S ribosomal protein L30 × 1 （C3SR32） 50S ribosomal protein L32 × 1 （A0A657HW13） ZN ZINC ION × 2 A2G 2-acetamido-2-deoxy-alpha-D-galactopyranose × 1 MG MAGNESIUM ION × 216 SPM SPERMINE × 1	ELECTRON MICROSCOPY cryo-EM buffer：pH 7.4 cryo-EM vitrification conditions：Cryogen ETHANE-PROPANE	Resolution 2.10 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB	Difference from Current Entry 8ANA	Assembly / Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Non-polymers	Method and Experimental Conditions	Structure Quality
8AKN Cryo-EM structure of the proline-rich antimicrobial peptide drosocin bound to the terminating ribosome Deposited 2022-07-30	Different oligomeric state Different ligand/ion Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 57-meric	Chain 0 1–55（55 aa）	Not recorded	ZN ZINC ION × 2 A2G 2-acetamido-2-deoxy-alpha-D-galactopyranose × 1 MG MAGNESIUM ION × 305 SPM SPERMINE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE-PROPANE	Resolution 2.30 Å

1 other PDB entries and 1 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence

UniProt name	A0A3S5YPG8_SALER
Isoform	—
PDB entities	1
Chains and sequence ranges	Author chain 0； PDBConstruct 1–55； UniProt 1–55

50S ribosomal protein L34

OrganismNot specified

UniProt A0A8X6EVB8

State in the Current Structure

Assembly	Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Associated Components	Method and Experimental Conditions	Structure Quality
1	Protein–RNA Heteromer Protein × 30 RNA 2 PDB declaration: 32-meric（32） Consistent with all polymer counts	Chain 1； UniProt 1–46	Not recorded	50S ribosomal protein L33 × 1 （A0A3S5YPG8） 50S ribosomal protein L35 × 1 （A0A8S0FT45） 50S ribosomal protein L36 × 1 （A0A2X1PTA3） 50S ribosomal protein L31 × 1 （C3SIV2） Drosocin1 × 1 （B4MNS1） 23S ribosomal RNA × 1 5S ribosomal RNA × 1 50S ribosomal protein L2 × 1 （A0A7L6YWH1） 50S ribosomal protein L3 × 1 （C3SQU2） 50S ribosomal protein L4 × 1 （D8A1N0） 50S ribosomal protein L5 × 1 （D6IEK6） 50S ribosomal protein L6 × 1 （C3SR17） 50S ribosomal protein L9 × 1 （C3SFP2） 50S ribosomal protein L13 × 1 （A0A656YGQ7） 50S ribosomal protein L14 × 1 （C3SQZ2） 50S ribosomal protein L15 × 1 （P02413） 50S ribosomal protein L16 × 1 （A0A0H3EMH6） 50S ribosomal protein L17 × 1 （A0A1X3JBW6） 50S ribosomal protein L18 × 1 （A0A514EKV0） 50S ribosomal protein L19 × 1 （A0A370V6W8） 50S ribosomal protein L20 × 1 （A0A378BCJ4） 50S ribosomal protein L21 × 1 （A0A0E2L2H1） 50S ribosomal protein L22 × 1 （A0A0F6B7N1） 50S ribosomal protein L23 × 1 （D7Y3D8） 50S ribosomal protein L24 × 1 （S1E9P3） 50S ribosomal protein L25 × 1 （P68919） 50S ribosomal protein L27 × 1 （C3SSG7） 50S ribosomal protein L28 × 1 （C3SME2） 50S ribosomal protein L29 × 1 （P0A7M6） 50S ribosomal protein L30 × 1 （C3SR32） 50S ribosomal protein L32 × 1 （A0A657HW13） ZN ZINC ION × 2 A2G 2-acetamido-2-deoxy-alpha-D-galactopyranose × 1 MG MAGNESIUM ION × 216 SPM SPERMINE × 1	ELECTRON MICROSCOPY cryo-EM buffer：pH 7.4 cryo-EM vitrification conditions：Cryogen ETHANE-PROPANE	Resolution 2.10 Å

Other States of the Same Protein in the Database

Other PDB	Difference from Current Entry 8ANA	Assembly / Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Non-polymers	Method and Experimental Conditions	Structure Quality
8AKN Cryo-EM structure of the proline-rich antimicrobial peptide drosocin bound to the terminating ribosome Deposited 2022-07-30	Different oligomeric state Different ligand/ion Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 57-meric	Chain 1 1–46（46 aa）	Not recorded	ZN ZINC ION × 2 A2G 2-acetamido-2-deoxy-alpha-D-galactopyranose × 1 MG MAGNESIUM ION × 305 SPM SPERMINE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE-PROPANE	Resolution 2.30 Å
8AM9 Cryo-EM structure of the proline-rich antimicrobial peptide drosocin bound to the elongating ribosome Deposited 2022-08-03	Different oligomeric state Different ligand/ion Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric	Chain 1 1–46（46 aa）	Not recorded	ZN ZINC ION × 2 MG MAGNESIUM ION × 293 SPM SPERMINE × 1 A2G 2-acetamido-2-deoxy-alpha-D-galactopyranose × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE-PROPANE	Resolution 2.80 Å

2 other PDB entries and 2 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence

UniProt name	A0A8X6EVB8_SALDU
Isoform	—
PDB entities	2
Chains and sequence ranges	Author chain 1； PDBConstruct 1–46； UniProt 1–46

50S ribosomal protein L35

OrganismNot specified

UniProt A0A8S0FT45

State in the Current Structure

Assembly	Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Associated Components	Method and Experimental Conditions	Structure Quality
1	Protein–RNA Heteromer Protein × 30 RNA 2 PDB declaration: 32-meric（32） Consistent with all polymer counts	Chain 2； UniProt 6–70	Not recorded	50S ribosomal protein L33 × 1 （A0A3S5YPG8） 50S ribosomal protein L34 × 1 （A0A8X6EVB8） 50S ribosomal protein L36 × 1 （A0A2X1PTA3） 50S ribosomal protein L31 × 1 （C3SIV2） Drosocin1 × 1 （B4MNS1） 23S ribosomal RNA × 1 5S ribosomal RNA × 1 50S ribosomal protein L2 × 1 （A0A7L6YWH1） 50S ribosomal protein L3 × 1 （C3SQU2） 50S ribosomal protein L4 × 1 （D8A1N0） 50S ribosomal protein L5 × 1 （D6IEK6） 50S ribosomal protein L6 × 1 （C3SR17） 50S ribosomal protein L9 × 1 （C3SFP2） 50S ribosomal protein L13 × 1 （A0A656YGQ7） 50S ribosomal protein L14 × 1 （C3SQZ2） 50S ribosomal protein L15 × 1 （P02413） 50S ribosomal protein L16 × 1 （A0A0H3EMH6） 50S ribosomal protein L17 × 1 （A0A1X3JBW6） 50S ribosomal protein L18 × 1 （A0A514EKV0） 50S ribosomal protein L19 × 1 （A0A370V6W8） 50S ribosomal protein L20 × 1 （A0A378BCJ4） 50S ribosomal protein L21 × 1 （A0A0E2L2H1） 50S ribosomal protein L22 × 1 （A0A0F6B7N1） 50S ribosomal protein L23 × 1 （D7Y3D8） 50S ribosomal protein L24 × 1 （S1E9P3） 50S ribosomal protein L25 × 1 （P68919） 50S ribosomal protein L27 × 1 （C3SSG7） 50S ribosomal protein L28 × 1 （C3SME2） 50S ribosomal protein L29 × 1 （P0A7M6） 50S ribosomal protein L30 × 1 （C3SR32） 50S ribosomal protein L32 × 1 （A0A657HW13） ZN ZINC ION × 2 A2G 2-acetamido-2-deoxy-alpha-D-galactopyranose × 1 MG MAGNESIUM ION × 216 SPM SPERMINE × 1	ELECTRON MICROSCOPY cryo-EM buffer：pH 7.4 cryo-EM vitrification conditions：Cryogen ETHANE-PROPANE	Resolution 2.10 Å

Other States of the Same Protein in the Database

Other PDB	Difference from Current Entry 8ANA	Assembly / Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Non-polymers	Method and Experimental Conditions	Structure Quality
8AKN Cryo-EM structure of the proline-rich antimicrobial peptide drosocin bound to the terminating ribosome Deposited 2022-07-30	Different oligomeric state Different ligand/ion Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 57-meric	Chain 2 6–70（65 aa）	Not recorded	ZN ZINC ION × 2 A2G 2-acetamido-2-deoxy-alpha-D-galactopyranose × 1 MG MAGNESIUM ION × 305 SPM SPERMINE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE-PROPANE	Resolution 2.30 Å
8AM9 Cryo-EM structure of the proline-rich antimicrobial peptide drosocin bound to the elongating ribosome Deposited 2022-08-03	Different oligomeric state Different ligand/ion Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric	Chain 2 6–70（65 aa）	Not recorded	ZN ZINC ION × 2 MG MAGNESIUM ION × 293 SPM SPERMINE × 1 A2G 2-acetamido-2-deoxy-alpha-D-galactopyranose × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE-PROPANE	Resolution 2.80 Å

2 other PDB entries and 2 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence

UniProt name	A0A8S0FT45_ECOLX
Isoform	—
PDB entities	3
Chains and sequence ranges	Author chain 2； PDBConstruct 1–65； UniProt 6–70

50S ribosomal protein L36

OrganismNot specified

UniProt A0A2X1PTA3

State in the Current Structure

Assembly	Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Associated Components	Method and Experimental Conditions	Structure Quality
1	Protein–RNA Heteromer Protein × 30 RNA 2 PDB declaration: 32-meric（32） Consistent with all polymer counts	Chain 3； UniProt 1–38	Not recorded	50S ribosomal protein L33 × 1 （A0A3S5YPG8） 50S ribosomal protein L34 × 1 （A0A8X6EVB8） 50S ribosomal protein L35 × 1 （A0A8S0FT45） 50S ribosomal protein L31 × 1 （C3SIV2） Drosocin1 × 1 （B4MNS1） 23S ribosomal RNA × 1 5S ribosomal RNA × 1 50S ribosomal protein L2 × 1 （A0A7L6YWH1） 50S ribosomal protein L3 × 1 （C3SQU2） 50S ribosomal protein L4 × 1 （D8A1N0） 50S ribosomal protein L5 × 1 （D6IEK6） 50S ribosomal protein L6 × 1 （C3SR17） 50S ribosomal protein L9 × 1 （C3SFP2） 50S ribosomal protein L13 × 1 （A0A656YGQ7） 50S ribosomal protein L14 × 1 （C3SQZ2） 50S ribosomal protein L15 × 1 （P02413） 50S ribosomal protein L16 × 1 （A0A0H3EMH6） 50S ribosomal protein L17 × 1 （A0A1X3JBW6） 50S ribosomal protein L18 × 1 （A0A514EKV0） 50S ribosomal protein L19 × 1 （A0A370V6W8） 50S ribosomal protein L20 × 1 （A0A378BCJ4） 50S ribosomal protein L21 × 1 （A0A0E2L2H1） 50S ribosomal protein L22 × 1 （A0A0F6B7N1） 50S ribosomal protein L23 × 1 （D7Y3D8） 50S ribosomal protein L24 × 1 （S1E9P3） 50S ribosomal protein L25 × 1 （P68919） 50S ribosomal protein L27 × 1 （C3SSG7） 50S ribosomal protein L28 × 1 （C3SME2） 50S ribosomal protein L29 × 1 （P0A7M6） 50S ribosomal protein L30 × 1 （C3SR32） 50S ribosomal protein L32 × 1 （A0A657HW13） ZN ZINC ION × 2 A2G 2-acetamido-2-deoxy-alpha-D-galactopyranose × 1 MG MAGNESIUM ION × 216 SPM SPERMINE × 1	ELECTRON MICROSCOPY cryo-EM buffer：pH 7.4 cryo-EM vitrification conditions：Cryogen ETHANE-PROPANE	Resolution 2.10 Å

Other States of the Same Protein in the Database

Other PDB	Difference from Current Entry 8ANA	Assembly / Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Non-polymers	Method and Experimental Conditions	Structure Quality
6ZTJ E. coli 70S-RNAP expressome complex in NusG-coupled state (38 nt intervening mRNA) Deposited 2020-07-20	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Other combination Heteromer；Protein × 58 PDB declaration: 66-meric	Chain B6 1–50（50 aa）	Not recorded	MG MAGNESIUM ION × 479 PHE PHENYLALANINE × 1 ZN ZINC ION × 4	ELECTRON MICROSCOPY cryo-EM buffer pH 8 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.40 Å
6ZTL E. coli 70S-RNAP expressome complex in collided state bound to NusG Deposited 2020-07-20	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Other combination Heteromer；Protein × 54 PDB declaration: 62-meric	Chain B6 1–50（50 aa）	Not recorded	MG MAGNESIUM ION × 441 PHE PHENYLALANINE × 1 ZN ZINC ION × 3	ELECTRON MICROSCOPY cryo-EM buffer pH 8 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.50 Å
6ZTM E. coli 70S-RNAP expressome complex in collided state without NusG Deposited 2020-07-20	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Other combination Heteromer；Protein × 52 PDB declaration: 60-meric	Chain B6 1–50（50 aa）	Not recorded	MG MAGNESIUM ION × 421 PHE PHENYLALANINE × 1 ZN ZINC ION × 3	ELECTRON MICROSCOPY cryo-EM buffer pH 8 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.30 Å
6ZTN E. coli 70S-RNAP expressome complex in NusG-coupled state (42 nt intervening mRNA) Deposited 2020-07-20	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Other combination Heteromer；Protein × 54 PDB declaration: 63-meric	Chain B6 1–50（50 aa）	Not recorded	MG MAGNESIUM ION × 487 PHE PHENYLALANINE × 1 ZN ZINC ION × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 8 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.90 Å
6ZTO E. coli 70S-RNAP expressome complex in uncoupled state 1 Deposited 2020-07-20	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Other combination Heteromer；Protein × 54 PDB declaration: 62-meric	Chain B6 1–50（50 aa）	Not recorded	MG MAGNESIUM ION × 487 PHE PHENYLALANINE × 1 ZN ZINC ION × 4	ELECTRON MICROSCOPY cryo-EM buffer pH 8 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.00 Å
6ZTP E. coli 70S-RNAP expressome complex in uncoupled state 6 Deposited 2020-07-20	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Other combination Heteromer；Protein × 54 PDB declaration: 62-meric	Chain B6 1–50（50 aa）	Not recorded	MG MAGNESIUM ION × 487 PHE PHENYLALANINE × 1 ZN ZINC ION × 4	ELECTRON MICROSCOPY cryo-EM buffer pH 8 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.00 Å
6ZU1 E. coli 70S-RNAP expressome complex in uncoupled state 2 Deposited 2020-07-21	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Other combination Heteromer；Protein × 54 PDB declaration: 62-meric	Chain B6 1–50（50 aa）	Not recorded	MG MAGNESIUM ION × 487 PHE PHENYLALANINE × 1 ZN ZINC ION × 4	ELECTRON MICROSCOPY cryo-EM buffer pH 8 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.00 Å
7Z20 70S E. coli ribosome with an extended uL23 loop from Candidatus marinimicrobia and a stalled filamin domain 5 nascent chain Deposited 2022-02-25	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 29 PDB declaration: 32-meric	Chain 8 1–50（50 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.29 Å
7ZOD 70S E. coli ribosome with an extended uL23 loop from Candidatus marinimicrobia Deposited 2022-04-25	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 28 PDB declaration: 31-meric	Chain 8 1–50（50 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.56 Å
7ZP8 70S E. coli ribosome with a stalled filamin domain 5 nascent chain Deposited 2022-04-26	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 29 PDB declaration: 32-meric	Chain 8 1–50（50 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.20 Å
7ZQ6 70S E. coli ribosome with truncated uL23 and uL24 loops and a stalled filamin domain 5 nascent chain Deposited 2022-04-29	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 29 PDB declaration: 32-meric	Chain 8 1–50（50 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.75 Å
8AKN Cryo-EM structure of the proline-rich antimicrobial peptide drosocin bound to the terminating ribosome Deposited 2022-07-30	Different oligomeric state Different ligand/ion Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 57-meric	Chain 3 1–38（38 aa）	Not recorded	ZN ZINC ION × 2 A2G 2-acetamido-2-deoxy-alpha-D-galactopyranose × 1 MG MAGNESIUM ION × 305 SPM SPERMINE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE-PROPANE	Resolution 2.30 Å
8AM9 Cryo-EM structure of the proline-rich antimicrobial peptide drosocin bound to the elongating ribosome Deposited 2022-08-03	Different oligomeric state Different ligand/ion Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric	Chain 3 1–38（38 aa）	Not recorded	ZN ZINC ION × 2 MG MAGNESIUM ION × 293 SPM SPERMINE × 1 A2G 2-acetamido-2-deoxy-alpha-D-galactopyranose × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE-PROPANE	Resolution 2.80 Å
9HUS Structure of WT E.coli ribosome with complexed filament nascent chain at length 31, with P-site tRNAs Deposited 2024-12-23	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 29 PDB declaration: 32-meric	Chain 8 1–50（50 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.40 Å
9HY6 Structure of E.coli ribosome with nascent chain at linker length of 31 amino acids, with mRNA, P-site and A-site tRNAs Deposited 2025-01-09	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 29 PDB declaration: 34-meric	Chain 8 1–50（50 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.60 Å
9HYM Structure of WT E.coli ribosome with complexed filament nascent chain at length 34, with mRNA, P-site and A-site tRNAs, and mRNA Deposited 2025-01-10	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 29 PDB declaration: 34-meric	Chain 8 1–50（50 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.20 Å
9I3L Structure of E.coli ribosome with filamin mutant Y719E nascent chain at linker length of 47 amino acids, with tRNA Deposited 2025-01-23	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 29 PDB declaration: 32-meric	Chain 8 1–50（50 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.10 Å

17 other PDB entries and 17 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence

UniProt name	A0A2X1PTA3_ECOLX
Isoform	—
PDB entities	4
Chains and sequence ranges	Author chain 3； PDBConstruct 1–38； UniProt 1–38

50S ribosomal protein L31

OrganismNot specified

UniProt C3SIV2

State in the Current Structure

Assembly	Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Associated Components	Method and Experimental Conditions	Structure Quality
1	Protein–RNA Heteromer Protein × 30 RNA 2 PDB declaration: 32-meric（32） Consistent with all polymer counts	Chain 4； UniProt 1–70	Not recorded	50S ribosomal protein L33 × 1 （A0A3S5YPG8） 50S ribosomal protein L34 × 1 （A0A8X6EVB8） 50S ribosomal protein L35 × 1 （A0A8S0FT45） 50S ribosomal protein L36 × 1 （A0A2X1PTA3） Drosocin1 × 1 （B4MNS1） 23S ribosomal RNA × 1 5S ribosomal RNA × 1 50S ribosomal protein L2 × 1 （A0A7L6YWH1） 50S ribosomal protein L3 × 1 （C3SQU2） 50S ribosomal protein L4 × 1 （D8A1N0） 50S ribosomal protein L5 × 1 （D6IEK6） 50S ribosomal protein L6 × 1 （C3SR17） 50S ribosomal protein L9 × 1 （C3SFP2） 50S ribosomal protein L13 × 1 （A0A656YGQ7） 50S ribosomal protein L14 × 1 （C3SQZ2） 50S ribosomal protein L15 × 1 （P02413） 50S ribosomal protein L16 × 1 （A0A0H3EMH6） 50S ribosomal protein L17 × 1 （A0A1X3JBW6） 50S ribosomal protein L18 × 1 （A0A514EKV0） 50S ribosomal protein L19 × 1 （A0A370V6W8） 50S ribosomal protein L20 × 1 （A0A378BCJ4） 50S ribosomal protein L21 × 1 （A0A0E2L2H1） 50S ribosomal protein L22 × 1 （A0A0F6B7N1） 50S ribosomal protein L23 × 1 （D7Y3D8） 50S ribosomal protein L24 × 1 （S1E9P3） 50S ribosomal protein L25 × 1 （P68919） 50S ribosomal protein L27 × 1 （C3SSG7） 50S ribosomal protein L28 × 1 （C3SME2） 50S ribosomal protein L29 × 1 （P0A7M6） 50S ribosomal protein L30 × 1 （C3SR32） 50S ribosomal protein L32 × 1 （A0A657HW13） ZN ZINC ION × 2 A2G 2-acetamido-2-deoxy-alpha-D-galactopyranose × 1 MG MAGNESIUM ION × 216 SPM SPERMINE × 1	ELECTRON MICROSCOPY cryo-EM buffer：pH 7.4 cryo-EM vitrification conditions：Cryogen ETHANE-PROPANE	Resolution 2.10 Å

Other States of the Same Protein in the Database

Other PDB	Difference from Current Entry 8ANA	Assembly / Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Non-polymers	Method and Experimental Conditions	Structure Quality
8AKN Cryo-EM structure of the proline-rich antimicrobial peptide drosocin bound to the terminating ribosome Deposited 2022-07-30	Different oligomeric state Different ligand/ion Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 57-meric	Chain 4 1–70（70 aa）	Not recorded	ZN ZINC ION × 2 A2G 2-acetamido-2-deoxy-alpha-D-galactopyranose × 1 MG MAGNESIUM ION × 305 SPM SPERMINE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE-PROPANE	Resolution 2.30 Å
8AM9 Cryo-EM structure of the proline-rich antimicrobial peptide drosocin bound to the elongating ribosome Deposited 2022-08-03	Different oligomeric state Different ligand/ion Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric	Chain 4 1–70（70 aa）	Not recorded	ZN ZINC ION × 2 MG MAGNESIUM ION × 293 SPM SPERMINE × 1 A2G 2-acetamido-2-deoxy-alpha-D-galactopyranose × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE-PROPANE	Resolution 2.80 Å
8VS9 Endogenous trans-translation complex with tmRNA*SmpB in the P site and alanyl-tRNA in the A site and deacyl-tRNA in the E site of E. coli 70S ribosome Deposited 2024-01-23	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 53 PDB declaration: 59-meric	Chain L31 1–45（45 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.90 Å
8VSA Endogenous trans-translation complex with tmRNA*SmpB in the P site and alanyl-tRNA in the A site of E. coli 70S ribosome Deposited 2024-01-23	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 53 PDB declaration: 58-meric	Chain L31 1–45（45 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.70 Å
9SX2 30S Doxycycline Bound E. coli Ribosome Deposited 2025-10-08	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 21 PDB declaration: 22-meric	Chain 4 1–67（67 aa）	Not recorded	ZN ZINC ION × 1 DXT (4S,4AR,5S,5AR,6R,12AS)-4-(DIMETHYLAMINO)-3,5,10,12,12A-PENTAHYDROXY-6-METHYL-1,11-DIOXO-1,4,4A,5,5A,6,11,12A-OCTAHYDROTETRACENE-2-CARBOXAMIDE × 1 MG MAGNESIUM ION × 142 K POTASSIUM ION × 4	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5；BS100 ribosomal resuspension buffer cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.08 Å

5 other PDB entries and 5 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence

UniProt name	C3SIV2_ECOLX
Isoform	—
PDB entities	5
Chains and sequence ranges	Author chain 4； PDBConstruct 1–70； UniProt 1–70

Drosocin1

OrganismNot specified

UniProt B4MNS1

State in the Current Structure

Assembly	Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Associated Components	Method and Experimental Conditions	Structure Quality
1	Protein–RNA Heteromer Protein × 30 RNA 2 PDB declaration: 32-meric（32） Consistent with all polymer counts	Chain A； UniProt 23–41	Not recorded	50S ribosomal protein L33 × 1 （A0A3S5YPG8） 50S ribosomal protein L34 × 1 （A0A8X6EVB8） 50S ribosomal protein L35 × 1 （A0A8S0FT45） 50S ribosomal protein L36 × 1 （A0A2X1PTA3） 50S ribosomal protein L31 × 1 （C3SIV2） 23S ribosomal RNA × 1 5S ribosomal RNA × 1 50S ribosomal protein L2 × 1 （A0A7L6YWH1） 50S ribosomal protein L3 × 1 （C3SQU2） 50S ribosomal protein L4 × 1 （D8A1N0） 50S ribosomal protein L5 × 1 （D6IEK6） 50S ribosomal protein L6 × 1 （C3SR17） 50S ribosomal protein L9 × 1 （C3SFP2） 50S ribosomal protein L13 × 1 （A0A656YGQ7） 50S ribosomal protein L14 × 1 （C3SQZ2） 50S ribosomal protein L15 × 1 （P02413） 50S ribosomal protein L16 × 1 （A0A0H3EMH6） 50S ribosomal protein L17 × 1 （A0A1X3JBW6） 50S ribosomal protein L18 × 1 （A0A514EKV0） 50S ribosomal protein L19 × 1 （A0A370V6W8） 50S ribosomal protein L20 × 1 （A0A378BCJ4） 50S ribosomal protein L21 × 1 （A0A0E2L2H1） 50S ribosomal protein L22 × 1 （A0A0F6B7N1） 50S ribosomal protein L23 × 1 （D7Y3D8） 50S ribosomal protein L24 × 1 （S1E9P3） 50S ribosomal protein L25 × 1 （P68919） 50S ribosomal protein L27 × 1 （C3SSG7） 50S ribosomal protein L28 × 1 （C3SME2） 50S ribosomal protein L29 × 1 （P0A7M6） 50S ribosomal protein L30 × 1 （C3SR32） 50S ribosomal protein L32 × 1 （A0A657HW13） ZN ZINC ION × 2 A2G 2-acetamido-2-deoxy-alpha-D-galactopyranose × 1 MG MAGNESIUM ION × 216 SPM SPERMINE × 1	ELECTRON MICROSCOPY cryo-EM buffer：pH 7.4 cryo-EM vitrification conditions：Cryogen ETHANE-PROPANE	Resolution 2.10 Å

Other States of the Same Protein in the Database

Other PDB	Difference from Current Entry 8ANA	Assembly / Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Non-polymers	Method and Experimental Conditions	Structure Quality
8AKN Cryo-EM structure of the proline-rich antimicrobial peptide drosocin bound to the terminating ribosome Deposited 2022-07-30	Different oligomeric state Different ligand/ion Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 57-meric	Chain A 23–41（19 aa）	Not recorded	ZN ZINC ION × 2 A2G 2-acetamido-2-deoxy-alpha-D-galactopyranose × 1 MG MAGNESIUM ION × 305 SPM SPERMINE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE-PROPANE	Resolution 2.30 Å
8AM9 Cryo-EM structure of the proline-rich antimicrobial peptide drosocin bound to the elongating ribosome Deposited 2022-08-03	Different oligomeric state Different ligand/ion Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric	Chain A 23–41（19 aa）	Not recorded	ZN ZINC ION × 2 MG MAGNESIUM ION × 293 SPM SPERMINE × 1 A2G 2-acetamido-2-deoxy-alpha-D-galactopyranose × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE-PROPANE	Resolution 2.80 Å

2 other PDB entries and 2 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence

UniProt name	B4MNS1_DROWI
Isoform	—
PDB entities	6
Chains and sequence ranges	Author chain A； PDBConstruct 1–19； UniProt 23–41

50S ribosomal protein L2

OrganismNot specified

UniProt A0A7L6YWH1

State in the Current Structure

Assembly	Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Associated Components	Method and Experimental Conditions	Structure Quality
1	Protein–RNA Heteromer Protein × 30 RNA 2 PDB declaration: 32-meric（32） Consistent with all polymer counts	Chain c； UniProt 1–273	Not recorded	50S ribosomal protein L33 × 1 （A0A3S5YPG8） 50S ribosomal protein L34 × 1 （A0A8X6EVB8） 50S ribosomal protein L35 × 1 （A0A8S0FT45） 50S ribosomal protein L36 × 1 （A0A2X1PTA3） 50S ribosomal protein L31 × 1 （C3SIV2） Drosocin1 × 1 （B4MNS1） 23S ribosomal RNA × 1 5S ribosomal RNA × 1 50S ribosomal protein L3 × 1 （C3SQU2） 50S ribosomal protein L4 × 1 （D8A1N0） 50S ribosomal protein L5 × 1 （D6IEK6） 50S ribosomal protein L6 × 1 （C3SR17） 50S ribosomal protein L9 × 1 （C3SFP2） 50S ribosomal protein L13 × 1 （A0A656YGQ7） 50S ribosomal protein L14 × 1 （C3SQZ2） 50S ribosomal protein L15 × 1 （P02413） 50S ribosomal protein L16 × 1 （A0A0H3EMH6） 50S ribosomal protein L17 × 1 （A0A1X3JBW6） 50S ribosomal protein L18 × 1 （A0A514EKV0） 50S ribosomal protein L19 × 1 （A0A370V6W8） 50S ribosomal protein L20 × 1 （A0A378BCJ4） 50S ribosomal protein L21 × 1 （A0A0E2L2H1） 50S ribosomal protein L22 × 1 （A0A0F6B7N1） 50S ribosomal protein L23 × 1 （D7Y3D8） 50S ribosomal protein L24 × 1 （S1E9P3） 50S ribosomal protein L25 × 1 （P68919） 50S ribosomal protein L27 × 1 （C3SSG7） 50S ribosomal protein L28 × 1 （C3SME2） 50S ribosomal protein L29 × 1 （P0A7M6） 50S ribosomal protein L30 × 1 （C3SR32） 50S ribosomal protein L32 × 1 （A0A657HW13） ZN ZINC ION × 2 A2G 2-acetamido-2-deoxy-alpha-D-galactopyranose × 1 MG MAGNESIUM ION × 216 SPM SPERMINE × 1	ELECTRON MICROSCOPY cryo-EM buffer：pH 7.4 cryo-EM vitrification conditions：Cryogen ETHANE-PROPANE	Resolution 2.10 Å

Other States of the Same Protein in the Database

Other PDB	Difference from Current Entry 8ANA	Assembly / Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Non-polymers	Method and Experimental Conditions	Structure Quality
8AKN Cryo-EM structure of the proline-rich antimicrobial peptide drosocin bound to the terminating ribosome Deposited 2022-07-30	Different oligomeric state Different ligand/ion Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 57-meric	Chain c 1–273（273 aa）	Not recorded	ZN ZINC ION × 2 A2G 2-acetamido-2-deoxy-alpha-D-galactopyranose × 1 MG MAGNESIUM ION × 305 SPM SPERMINE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE-PROPANE	Resolution 2.30 Å
8AM9 Cryo-EM structure of the proline-rich antimicrobial peptide drosocin bound to the elongating ribosome Deposited 2022-08-03	Different oligomeric state Different ligand/ion Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric	Chain c 1–273（273 aa）	Not recorded	ZN ZINC ION × 2 MG MAGNESIUM ION × 293 SPM SPERMINE × 1 A2G 2-acetamido-2-deoxy-alpha-D-galactopyranose × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE-PROPANE	Resolution 2.80 Å

2 other PDB entries and 2 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence

UniProt name	A0A7L6YWH1_9ENTR
Isoform	—
PDB entities	9
Chains and sequence ranges	Author chain c； PDBConstruct 1–273； UniProt 1–273

50S ribosomal protein L3

OrganismNot specified

UniProt C3SQU2

State in the Current Structure

Assembly	Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Associated Components	Method and Experimental Conditions	Structure Quality
1	Protein–RNA Heteromer Protein × 30 RNA 2 PDB declaration: 32-meric（32） Consistent with all polymer counts	Chain d； UniProt 1–209	Non-standard monomer：Yes (specific site not provided by mmCIF)	50S ribosomal protein L33 × 1 （A0A3S5YPG8） 50S ribosomal protein L34 × 1 （A0A8X6EVB8） 50S ribosomal protein L35 × 1 （A0A8S0FT45） 50S ribosomal protein L36 × 1 （A0A2X1PTA3） 50S ribosomal protein L31 × 1 （C3SIV2） Drosocin1 × 1 （B4MNS1） 23S ribosomal RNA × 1 5S ribosomal RNA × 1 50S ribosomal protein L2 × 1 （A0A7L6YWH1） 50S ribosomal protein L4 × 1 （D8A1N0） 50S ribosomal protein L5 × 1 （D6IEK6） 50S ribosomal protein L6 × 1 （C3SR17） 50S ribosomal protein L9 × 1 （C3SFP2） 50S ribosomal protein L13 × 1 （A0A656YGQ7） 50S ribosomal protein L14 × 1 （C3SQZ2） 50S ribosomal protein L15 × 1 （P02413） 50S ribosomal protein L16 × 1 （A0A0H3EMH6） 50S ribosomal protein L17 × 1 （A0A1X3JBW6） 50S ribosomal protein L18 × 1 （A0A514EKV0） 50S ribosomal protein L19 × 1 （A0A370V6W8） 50S ribosomal protein L20 × 1 （A0A378BCJ4） 50S ribosomal protein L21 × 1 （A0A0E2L2H1） 50S ribosomal protein L22 × 1 （A0A0F6B7N1） 50S ribosomal protein L23 × 1 （D7Y3D8） 50S ribosomal protein L24 × 1 （S1E9P3） 50S ribosomal protein L25 × 1 （P68919） 50S ribosomal protein L27 × 1 （C3SSG7） 50S ribosomal protein L28 × 1 （C3SME2） 50S ribosomal protein L29 × 1 （P0A7M6） 50S ribosomal protein L30 × 1 （C3SR32） 50S ribosomal protein L32 × 1 （A0A657HW13） ZN ZINC ION × 2 A2G 2-acetamido-2-deoxy-alpha-D-galactopyranose × 1 MG MAGNESIUM ION × 216 SPM SPERMINE × 1	ELECTRON MICROSCOPY cryo-EM buffer：pH 7.4 cryo-EM vitrification conditions：Cryogen ETHANE-PROPANE	Resolution 2.10 Å

Other States of the Same Protein in the Database

Other PDB	Difference from Current Entry 8ANA	Assembly / Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Non-polymers	Method and Experimental Conditions	Structure Quality
11BQ Escherichia coli 70S ribosome containing an evolved 16S rRNA (EC-S3.5) Deposited 2026-02-16	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric	Chain d 1–209（209 aa）	Non-standard monomer：Yes (specific site not provided by mmCIF)	ZN ZINC ION × 2 MG MAGNESIUM ION × 307 SPD SPERMIDINE × 14 SPM SPERMINE × 1 C CYTIDINE-5'-MONOPHOSPHATE × 1 G GUANOSINE-5'-MONOPHOSPHATE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.00 Å
11DG Chimeric Escherichia coli 70S ribosome containing an evolved Vibrio cholerae 16S rRNA (VC-S4.4) Deposited 2026-02-18	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric	Chain d 1–209（209 aa）	Non-standard monomer：Yes (specific site not provided by mmCIF)	ZN ZINC ION × 2 MG MAGNESIUM ION × 272 SPD SPERMIDINE × 14 SPM SPERMINE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.00 Å
11EL Chimeric Escherichia coli 70S ribosome containing an evolved 16S rRNA from Vibrio cholerae (VC-ST) Deposited 2026-02-19	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric	Chain d 1–209（209 aa）	Non-standard monomer：Yes (specific site not provided by mmCIF)	ZN ZINC ION × 2 MG MAGNESIUM ION × 308 SPD SPERMIDINE × 16 SPM SPERMINE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.20 Å
4V85 Crystal Structure of Release Factor RF3 Trapped in the GTP State on a Rotated Conformation of the Ribosome. Deposited 2011-06-13	Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 55 PDB declaration: 59-meric	Chain BD 1–209（209 aa）	Not recorded	MG MAGNESIUM ION × 493 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；295 K；Tris Ac PH.7.0, 25-35 mM KCL, 6.1% PEG 20000, 1% glycerol, 50mM sucrose, VAPOR DIFFUSION, SITTING DROP, temperature 295K	Resolution 3.20 Å R-free 0.250
7YLA Cryo-EM structure of 50S-HflX complex Deposited 2022-07-25	Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 30 PDB declaration: 32-meric	Chain L 1–209（209 aa）	Not recorded	GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 3 NA SODIUM ION × 1 ZN ZINC ION × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.52 Å
8AM9 Cryo-EM structure of the proline-rich antimicrobial peptide drosocin bound to the elongating ribosome Deposited 2022-08-03	Different oligomeric state Different ligand/ion Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric	Chain d 1–209（209 aa）	Non-standard monomer：Yes (specific site not provided by mmCIF)	ZN ZINC ION × 2 MG MAGNESIUM ION × 293 SPM SPERMINE × 1 A2G 2-acetamido-2-deoxy-alpha-D-galactopyranose × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE-PROPANE	Resolution 2.80 Å
8EIU E. coli 70S ribosome with A-loop mutations U2554C and U2555C Deposited 2022-09-15	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric	Chain d 1–209（209 aa）	Non-standard monomer：Yes (specific site not provided by mmCIF)	ZN ZINC ION × 2 PAR PAROMOMYCIN × 1 MG MAGNESIUM ION × 268 SPD SPERMIDINE × 16 8AN 3'-amino-3'-deoxyadenosine 5'-(dihydrogen phosphate) × 2 SPM SPERMINE × 1 MET METHIONINE × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.24 Å
8J1Z The global structure of pre50S related to DbpA in state3 Deposited 2023-04-13	Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 25 PDB declaration: 27-meric	Chain d 1–209（209 aa）	Not recorded	MG MAGNESIUM ION × 210	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5；20mM HEPES-KOH,pH7.5;140mM NH4Cl;10mM Mg2+,1mM DTT cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.60 Å
8QBT E. coli ApdP-stalled ribosomal complex Deposited 2023-08-25	Different mutation/modification Different oligomeric state Different ligand/ion Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 45 PDB declaration: 52-meric	Chain D 1–209（209 aa）	Not recorded	MG MAGNESIUM ION × 248 K POTASSIUM ION × 127 PRO PROLINE × 1 ZN ZINC ION × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE-PROPANE	Resolution 2.20 Å
8W51 Structure of YchF(H114A) on E.coli 50S ribosomal subunit Deposited 2023-08-25	Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 30 PDB declaration: 32-meric	Chain D 1–209（209 aa）	Not recorded	MG MAGNESIUM ION × 104 ZN ZINC ION × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.40 Å
8ZEJ Structure of E.coli ribosome in complex with an engineered arrest peptide Deposited 2024-05-06	Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric	Chain d 1–209（209 aa）	Not recorded	ZN ZINC ION × 1 PRO PROLINE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6；20 mM HEPES (pH 7.6), 140 mM NH4Cl, 25 mM MgCl2, 2 mM DTT cryo-EM vitrification conditions Cryogen NITROGEN	Resolution 2.69 Å
8ZFI Structure of E.coli ribosome in complex with an engineered arrest peptide and trigger factor Deposited 2024-05-07	Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 57-meric	Chain d 1–209（209 aa）	Not recorded	ZN ZINC ION × 1 PRO PROLINE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6；20 mM HEPES (pH 7.6), 140 mM NH4Cl, 25 mM MgCl2, 2 mM DTT cryo-EM vitrification conditions Cryogen NITROGEN	Resolution 2.90 Å
9AX7 70S initiation complex (tRNA-fMet M1 + CUG start codon) Deposited 2024-03-06	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 54-meric	Chain d 1–209（209 aa）	Non-standard monomer：Yes (specific site not provided by mmCIF)	ZN ZINC ION × 2 MG MAGNESIUM ION × 309 IAS BETA-L-ASPARTIC ACID × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.63 Å
9CG5 70S initiation complex (tRNA-fMet M1 + UUG start codon) Deposited 2024-06-28	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 54-meric	Chain d 1–209（209 aa）	Non-standard monomer：Yes (specific site not provided by mmCIF)	ZN ZINC ION × 2 MG MAGNESIUM ION × 309 IAS BETA-L-ASPARTIC ACID × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.59 Å
9CG6 70S initiation complex (tRNA-fMet M1 + GUG start codon) Deposited 2024-06-28	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 54-meric	Chain d 1–209（209 aa）	Non-standard monomer：Yes (specific site not provided by mmCIF)	ZN ZINC ION × 2 MG MAGNESIUM ION × 309 IAS BETA-L-ASPARTIC ACID × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.61 Å
9CG7 70S initiation complex (tRNA-fMet M1 + AUG start codon) Deposited 2024-06-28	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 54-meric	Chain d 1–209（209 aa）	Non-standard monomer：Yes (specific site not provided by mmCIF)	ZN ZINC ION × 2 MG MAGNESIUM ION × 309 IAS BETA-L-ASPARTIC ACID × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.75 Å
9D89 E. coli 50S ribosomal subunit in complex with PrAMP rumicidin-2 (focused refinement) Deposited 2024-08-19	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 29 PDB declaration: 31-meric	Chain G 1–209（209 aa）	Non-standard monomer：Yes (specific site not provided by mmCIF)	ZN ZINC ION × 1 MG MAGNESIUM ION × 204 K POTASSIUM ION × 93	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 1.95 Å
9O2X Structure of WT E.coli ribosome 70S subunit with complexed with mRNA, P-site fMet-NH-tRNAfMet and A-site (S)-betahydroxyBocK charged NH-tRNAPyl Deposited 2025-04-04	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric	Chain d 1–209（209 aa）	Non-standard monomer：Yes (specific site not provided by mmCIF)	ZN ZINC ION × 2 MG MAGNESIUM ION × 320 FME N-FORMYLMETHIONINE × 1 K POTASSIUM ION × 10 PAR PAROMOMYCIN × 1 SPD SPERMIDINE × 14 SPM SPERMINE × 1 A1B71 (2S)-6-[(tert-butoxycarbonyl)amino]-2-(hydroxymethyl)hexanoic acid × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.29 Å
9O2Y Structure of WT E.coli ribosome 70S subunit with complexed with mRNA, P-site fMet-NH-tRNAfMet and A-site (R) beta-2-hydroxy-BocLysine acid charged NH-tRNAPyl Deposited 2025-04-04	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric	Chain d 1–209（209 aa）	Non-standard monomer：Yes (specific site not provided by mmCIF)	PAR PAROMOMYCIN × 1 MG MAGNESIUM ION × 320 SPD SPERMIDINE × 13 FME N-FORMYLMETHIONINE × 1 SPM SPERMINE × 1 K POTASSIUM ION × 10 ZN ZINC ION × 2 A1B70 (2R)-6-[(tert-butoxycarbonyl)amino]-2-(hydroxymethyl)hexanoic acid × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.14 Å
9PIH E. coli 70S ribosome bound to Doxycycline Deposited 2025-07-10	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 54-meric	Chain d 1–209（209 aa）	Non-standard monomer：Yes (specific site not provided by mmCIF)	ZN ZINC ION × 1 MG MAGNESIUM ION × 276 K POTASSIUM ION × 92 DXT (4S,4AR,5S,5AR,6R,12AS)-4-(DIMETHYLAMINO)-3,5,10,12,12A-PENTAHYDROXY-6-METHYL-1,11-DIOXO-1,4,4A,5,5A,6,11,12A-OCTAHYDROTETRACENE-2-CARBOXAMIDE × 6	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.40 Å
9PII E. coli 70S ribosome bound to Sarecycline Deposited 2025-07-10	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 54-meric	Chain d 1–209（209 aa）	Non-standard monomer：Yes (specific site not provided by mmCIF)	ZN ZINC ION × 1 MG MAGNESIUM ION × 281 K POTASSIUM ION × 100 V7A Sarecycline × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.40 Å
9PIJ E. coli 70S ribosome bound to Minocycline Deposited 2025-07-10	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 54-meric	Chain d 1–209（209 aa）	Non-standard monomer：Yes (specific site not provided by mmCIF)	ZN ZINC ION × 1 MG MAGNESIUM ION × 340 K POTASSIUM ION × 148 MIY (4S,4AS,5AR,12AS)-4,7-BIS(DIMETHYLAMINO)-3,10,12,12A-TETRAHYDROXY-1,11-DIOXO-1,4,4A,5,5A,6,11,12A-OCTAHYDROTETRACENE-2- CARBOXAMIDE × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.20 Å
9WNQ Structure of E.coli ribosome in complex with an engineered arrest peptide Deposited 2025-09-05	Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric	Chain d 1–209（209 aa）	Not recorded	ZN ZINC ION × 1 PRO PROLINE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6；20 mM HEPES (pH 7.6), 140 mM NH4Cl, 25 mM MgCl2, 2 mM DTT cryo-EM vitrification conditions Cryogen NITROGEN	Resolution 2.59 Å
9WNR Structure of E.coli ribosome in complex with an engineered arrest peptide and trigger factor Deposited 2025-09-05	Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 57-meric	Chain d 1–209（209 aa）	Not recorded	ZN ZINC ION × 1 PRO PROLINE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6；20 mM HEPES (pH 7.6), 140 mM NH4Cl, 25 mM MgCl2, 2 mM DTT cryo-EM vitrification conditions Cryogen NITROGEN	Resolution 2.76 Å

24 other PDB entries and 24 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence

UniProt name	C3SQU2_ECOLX
Isoform	—
PDB entities	10
Chains and sequence ranges	Author chain d； PDBConstruct 1–209； UniProt 1–209

50S ribosomal protein L4

OrganismNot specified

UniProt D8A1N0

State in the Current Structure

Assembly	Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Associated Components	Method and Experimental Conditions	Structure Quality
1	Protein–RNA Heteromer Protein × 30 RNA 2 PDB declaration: 32-meric（32） Consistent with all polymer counts	Chain e； UniProt 1–201	Not recorded	50S ribosomal protein L33 × 1 （A0A3S5YPG8） 50S ribosomal protein L34 × 1 （A0A8X6EVB8） 50S ribosomal protein L35 × 1 （A0A8S0FT45） 50S ribosomal protein L36 × 1 （A0A2X1PTA3） 50S ribosomal protein L31 × 1 （C3SIV2） Drosocin1 × 1 （B4MNS1） 23S ribosomal RNA × 1 5S ribosomal RNA × 1 50S ribosomal protein L2 × 1 （A0A7L6YWH1） 50S ribosomal protein L3 × 1 （C3SQU2） 50S ribosomal protein L5 × 1 （D6IEK6） 50S ribosomal protein L6 × 1 （C3SR17） 50S ribosomal protein L9 × 1 （C3SFP2） 50S ribosomal protein L13 × 1 （A0A656YGQ7） 50S ribosomal protein L14 × 1 （C3SQZ2） 50S ribosomal protein L15 × 1 （P02413） 50S ribosomal protein L16 × 1 （A0A0H3EMH6） 50S ribosomal protein L17 × 1 （A0A1X3JBW6） 50S ribosomal protein L18 × 1 （A0A514EKV0） 50S ribosomal protein L19 × 1 （A0A370V6W8） 50S ribosomal protein L20 × 1 （A0A378BCJ4） 50S ribosomal protein L21 × 1 （A0A0E2L2H1） 50S ribosomal protein L22 × 1 （A0A0F6B7N1） 50S ribosomal protein L23 × 1 （D7Y3D8） 50S ribosomal protein L24 × 1 （S1E9P3） 50S ribosomal protein L25 × 1 （P68919） 50S ribosomal protein L27 × 1 （C3SSG7） 50S ribosomal protein L28 × 1 （C3SME2） 50S ribosomal protein L29 × 1 （P0A7M6） 50S ribosomal protein L30 × 1 （C3SR32） 50S ribosomal protein L32 × 1 （A0A657HW13） ZN ZINC ION × 2 A2G 2-acetamido-2-deoxy-alpha-D-galactopyranose × 1 MG MAGNESIUM ION × 216 SPM SPERMINE × 1	ELECTRON MICROSCOPY cryo-EM buffer：pH 7.4 cryo-EM vitrification conditions：Cryogen ETHANE-PROPANE	Resolution 2.10 Å

Other States of the Same Protein in the Database

Other PDB	Difference from Current Entry 8ANA	Assembly / Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Non-polymers	Method and Experimental Conditions	Structure Quality
8AKN Cryo-EM structure of the proline-rich antimicrobial peptide drosocin bound to the terminating ribosome Deposited 2022-07-30	Different oligomeric state Different ligand/ion Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 57-meric	Chain e 1–201（201 aa）	Not recorded	ZN ZINC ION × 2 A2G 2-acetamido-2-deoxy-alpha-D-galactopyranose × 1 MG MAGNESIUM ION × 305 SPM SPERMINE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE-PROPANE	Resolution 2.30 Å
8AM9 Cryo-EM structure of the proline-rich antimicrobial peptide drosocin bound to the elongating ribosome Deposited 2022-08-03	Different oligomeric state Different ligand/ion Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric	Chain e 1–201（201 aa）	Not recorded	ZN ZINC ION × 2 MG MAGNESIUM ION × 293 SPM SPERMINE × 1 A2G 2-acetamido-2-deoxy-alpha-D-galactopyranose × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE-PROPANE	Resolution 2.80 Å

2 other PDB entries and 2 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence

UniProt name	D8A1N0_ECOMS
Isoform	—
PDB entities	11
Chains and sequence ranges	Author chain e； PDBConstruct 1–201； UniProt 1–201

50S ribosomal protein L5

OrganismNot specified

UniProt D6IEK6

State in the Current Structure

Assembly	Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Associated Components	Method and Experimental Conditions	Structure Quality
1	Protein–RNA Heteromer Protein × 30 RNA 2 PDB declaration: 32-meric（32） Consistent with all polymer counts	Chain f； UniProt 1–179	Not recorded	50S ribosomal protein L33 × 1 （A0A3S5YPG8） 50S ribosomal protein L34 × 1 （A0A8X6EVB8） 50S ribosomal protein L35 × 1 （A0A8S0FT45） 50S ribosomal protein L36 × 1 （A0A2X1PTA3） 50S ribosomal protein L31 × 1 （C3SIV2） Drosocin1 × 1 （B4MNS1） 23S ribosomal RNA × 1 5S ribosomal RNA × 1 50S ribosomal protein L2 × 1 （A0A7L6YWH1） 50S ribosomal protein L3 × 1 （C3SQU2） 50S ribosomal protein L4 × 1 （D8A1N0） 50S ribosomal protein L6 × 1 （C3SR17） 50S ribosomal protein L9 × 1 （C3SFP2） 50S ribosomal protein L13 × 1 （A0A656YGQ7） 50S ribosomal protein L14 × 1 （C3SQZ2） 50S ribosomal protein L15 × 1 （P02413） 50S ribosomal protein L16 × 1 （A0A0H3EMH6） 50S ribosomal protein L17 × 1 （A0A1X3JBW6） 50S ribosomal protein L18 × 1 （A0A514EKV0） 50S ribosomal protein L19 × 1 （A0A370V6W8） 50S ribosomal protein L20 × 1 （A0A378BCJ4） 50S ribosomal protein L21 × 1 （A0A0E2L2H1） 50S ribosomal protein L22 × 1 （A0A0F6B7N1） 50S ribosomal protein L23 × 1 （D7Y3D8） 50S ribosomal protein L24 × 1 （S1E9P3） 50S ribosomal protein L25 × 1 （P68919） 50S ribosomal protein L27 × 1 （C3SSG7） 50S ribosomal protein L28 × 1 （C3SME2） 50S ribosomal protein L29 × 1 （P0A7M6） 50S ribosomal protein L30 × 1 （C3SR32） 50S ribosomal protein L32 × 1 （A0A657HW13） ZN ZINC ION × 2 A2G 2-acetamido-2-deoxy-alpha-D-galactopyranose × 1 MG MAGNESIUM ION × 216 SPM SPERMINE × 1	ELECTRON MICROSCOPY cryo-EM buffer：pH 7.4 cryo-EM vitrification conditions：Cryogen ETHANE-PROPANE	Resolution 2.10 Å

Other States of the Same Protein in the Database

Other PDB	Difference from Current Entry 8ANA	Assembly / Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Non-polymers	Method and Experimental Conditions	Structure Quality
8AKN Cryo-EM structure of the proline-rich antimicrobial peptide drosocin bound to the terminating ribosome Deposited 2022-07-30	Different oligomeric state Different ligand/ion Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 57-meric	Chain f 1–179（179 aa）	Not recorded	ZN ZINC ION × 2 A2G 2-acetamido-2-deoxy-alpha-D-galactopyranose × 1 MG MAGNESIUM ION × 305 SPM SPERMINE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE-PROPANE	Resolution 2.30 Å
8AM9 Cryo-EM structure of the proline-rich antimicrobial peptide drosocin bound to the elongating ribosome Deposited 2022-08-03	Different oligomeric state Different ligand/ion Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric	Chain f 1–179（179 aa）	Not recorded	ZN ZINC ION × 2 MG MAGNESIUM ION × 293 SPM SPERMINE × 1 A2G 2-acetamido-2-deoxy-alpha-D-galactopyranose × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE-PROPANE	Resolution 2.80 Å
8G2U Time-resolved cryo-EM study of the 70S recycling by the HflX:control-apo-70S at 900ms Deposited 2023-02-06	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 51-meric	Chain F 2–179（178 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.00 Å
8G31 Time-resolved cryo-EM study of the 70S recycling by the HflX:2nd Intermediate Deposited 2023-02-06	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 52-meric	Chain F 2–179（178 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.20 Å
8G34 Time-resolved cryo-EM study of the 70S recycling by the HflX:1st intermediate Deposited 2023-02-06	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 52-meric	Chain F 2–179（178 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.20 Å
8G38 Time-resolved cryo-EM study of the 70S recycling by the HflX:3rd Intermediate Deposited 2023-02-07	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 52-meric	Chain F 2–179（178 aa）	Not recorded	GTP GUANOSINE-5'-TRIPHOSPHATE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.20 Å
8T5D Cryo-EM studies of the interplay between uS2 ribosomal protein and leaderless mRNA during bacterial translation initiation Deposited 2023-06-13	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 53-meric	Chain F 2–179（178 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.20 Å
8T5H Cryo-EM studies of the interplay between uS2 ribosomal protein and leaderless mRNA during bacterial translation initiation Deposited 2023-06-13	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 46 PDB declaration: 51-meric	Chain F 2–179（178 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.30 Å

8 other PDB entries and 8 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence

UniProt name	D6IEK6_ECOLX
Isoform	—
PDB entities	12
Chains and sequence ranges	Author chain f； PDBConstruct 1–179； UniProt 1–179

50S ribosomal protein L6

OrganismNot specified

UniProt C3SR17

State in the Current Structure

Assembly	Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Associated Components	Method and Experimental Conditions	Structure Quality
1	Protein–RNA Heteromer Protein × 30 RNA 2 PDB declaration: 32-meric（32） Consistent with all polymer counts	Chain g； UniProt 1–177	Not recorded	50S ribosomal protein L33 × 1 （A0A3S5YPG8） 50S ribosomal protein L34 × 1 （A0A8X6EVB8） 50S ribosomal protein L35 × 1 （A0A8S0FT45） 50S ribosomal protein L36 × 1 （A0A2X1PTA3） 50S ribosomal protein L31 × 1 （C3SIV2） Drosocin1 × 1 （B4MNS1） 23S ribosomal RNA × 1 5S ribosomal RNA × 1 50S ribosomal protein L2 × 1 （A0A7L6YWH1） 50S ribosomal protein L3 × 1 （C3SQU2） 50S ribosomal protein L4 × 1 （D8A1N0） 50S ribosomal protein L5 × 1 （D6IEK6） 50S ribosomal protein L9 × 1 （C3SFP2） 50S ribosomal protein L13 × 1 （A0A656YGQ7） 50S ribosomal protein L14 × 1 （C3SQZ2） 50S ribosomal protein L15 × 1 （P02413） 50S ribosomal protein L16 × 1 （A0A0H3EMH6） 50S ribosomal protein L17 × 1 （A0A1X3JBW6） 50S ribosomal protein L18 × 1 （A0A514EKV0） 50S ribosomal protein L19 × 1 （A0A370V6W8） 50S ribosomal protein L20 × 1 （A0A378BCJ4） 50S ribosomal protein L21 × 1 （A0A0E2L2H1） 50S ribosomal protein L22 × 1 （A0A0F6B7N1） 50S ribosomal protein L23 × 1 （D7Y3D8） 50S ribosomal protein L24 × 1 （S1E9P3） 50S ribosomal protein L25 × 1 （P68919） 50S ribosomal protein L27 × 1 （C3SSG7） 50S ribosomal protein L28 × 1 （C3SME2） 50S ribosomal protein L29 × 1 （P0A7M6） 50S ribosomal protein L30 × 1 （C3SR32） 50S ribosomal protein L32 × 1 （A0A657HW13） ZN ZINC ION × 2 A2G 2-acetamido-2-deoxy-alpha-D-galactopyranose × 1 MG MAGNESIUM ION × 216 SPM SPERMINE × 1	ELECTRON MICROSCOPY cryo-EM buffer：pH 7.4 cryo-EM vitrification conditions：Cryogen ETHANE-PROPANE	Resolution 2.10 Å

Other States of the Same Protein in the Database

Other PDB	Difference from Current Entry 8ANA	Assembly / Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Non-polymers	Method and Experimental Conditions	Structure Quality
4V85 Crystal Structure of Release Factor RF3 Trapped in the GTP State on a Rotated Conformation of the Ribosome. Deposited 2011-06-13	Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 55 PDB declaration: 59-meric	Chain BG 1–177（177 aa）	Not recorded	MG MAGNESIUM ION × 493 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；295 K；Tris Ac PH.7.0, 25-35 mM KCL, 6.1% PEG 20000, 1% glycerol, 50mM sucrose, VAPOR DIFFUSION, SITTING DROP, temperature 295K	Resolution 3.20 Å R-free 0.250
7YLA Cryo-EM structure of 50S-HflX complex Deposited 2022-07-25	Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 30 PDB declaration: 32-meric	Chain O 2–177（176 aa）	Not recorded	GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 3 NA SODIUM ION × 1 ZN ZINC ION × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.52 Å
8AKN Cryo-EM structure of the proline-rich antimicrobial peptide drosocin bound to the terminating ribosome Deposited 2022-07-30	Different oligomeric state Different ligand/ion Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 57-meric	Chain g 1–177（177 aa）	Not recorded	ZN ZINC ION × 2 A2G 2-acetamido-2-deoxy-alpha-D-galactopyranose × 1 MG MAGNESIUM ION × 305 SPM SPERMINE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE-PROPANE	Resolution 2.30 Å
8AM9 Cryo-EM structure of the proline-rich antimicrobial peptide drosocin bound to the elongating ribosome Deposited 2022-08-03	Different oligomeric state Different ligand/ion Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric	Chain g 1–177（177 aa）	Not recorded	ZN ZINC ION × 2 MG MAGNESIUM ION × 293 SPM SPERMINE × 1 A2G 2-acetamido-2-deoxy-alpha-D-galactopyranose × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE-PROPANE	Resolution 2.80 Å
8EKC Escherichia coli 70S ribosome bound to thermorubin, deacylated P-site tRNAfMet and aminoacylated A-site Phe-tRNA Deposited 2022-09-20	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric	Chain G 1–177（177 aa）	Not recorded	MG MAGNESIUM ION × 714 T8B Thermorubin × 1 PHE PHENYLALANINE × 1 ZN ZINC ION × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.70 Å
8FZD Cryo-EM structure of an E. coli non-rotated ribosome termination complex bound with apoRF3, RF1, P- and E-site tRNAPhe (Composite state I-B) Deposited 2023-01-28	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 53 PDB declaration: 59-meric	Chain G 1–177（177 aa）	Not recorded	MG MAGNESIUM ION × 581 ZN ZINC ION × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4；5 mM Tris-HCl, 60 mM NH4Cl, 10 mM MgCl2, 6 mM B-mercaptoethanol cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.10 Å
8FZE Cryo-EM structure of an E. coli non-rotated ribosome termination complex bound with RF1, P- and E-site tRNAPhe (State I-A) Deposited 2023-01-28	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 58-meric	Chain G 1–177（177 aa）	Not recorded	MG MAGNESIUM ION × 570 ZN ZINC ION × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4；5 mM Tris-HCl, 60 mM NH4Cl, 10 mM MgCl2, 6 mM B-mercaptoethanol cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.00 Å
8FZF Cryo-EM structure of an E. coli rotated ribosome complex bound with RF3-ppGpp and p/E-tRNAPhe (Composite state I-C) Deposited 2023-01-28	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 56-meric	Chain G 1–177（177 aa）	Not recorded	MG MAGNESIUM ION × 384 G4P GUANOSINE-5',3'-TETRAPHOSPHATE × 1 ZN ZINC ION × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4；5 mM Tris-HCl, 60 mM NH4Cl, 10 mM MgCl2, 6 mM B-mercaptoethanol cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.20 Å
8FZG Cryo-EM structure of an E. coli non-rotated ribosome termination complex bound with RF3-GDPCP, RF1, P- and E-site tRNAPhe (Composite state II-A) Deposited 2023-01-28	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 53 PDB declaration: 59-meric	Chain G 1–177（177 aa）	Not recorded	MG MAGNESIUM ION × 428 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 ZN ZINC ION × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4；5 mM Tris-HCl, 60 mM NH4Cl, 10 mM MgCl2, 6 mM B-mercaptoethanol cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.10 Å
8FZH Cryo-EM structure of an E. coli non-rotated ribosome termination complex bound with RF1, P- and E-site tRNAPhe (State II-D) Deposited 2023-01-28	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 58-meric	Chain G 1–177（177 aa）	Not recorded	MG MAGNESIUM ION × 595 ZN ZINC ION × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4；5 mM Tris-HCl, 60 mM NH4Cl, 10 mM MgCl2, 6 mM B-mercaptoethanol cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.90 Å
8FZI Cryo-EM structure of an E. coli rotated ribosome bound with RF3-GDPCP and p/E-tRNAPhe (Composite state II-B) Deposited 2023-01-28	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 56-meric	Chain G 1–177（177 aa）	Not recorded	MG MAGNESIUM ION × 737 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 ZN ZINC ION × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4；5 mM Tris-HCl, 60 mM NH4Cl, 10 mM MgCl2, 6 mM B-mercaptoethanol cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.10 Å
8FZJ Cryo-EM structure of an E. coli rotated ribosome bound with RF3-GDPCP and p/E-tRNAPhe (Composite state II-C) Deposited 2023-01-28	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 56-meric	Chain G 1–177（177 aa）	Not recorded	MG MAGNESIUM ION × 757 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 ZN ZINC ION × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4；5 mM Tris-HCl, 60 mM NH4Cl, 10 mM MgCl2, 6 mM B-mercaptoethanol cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.00 Å
8G7P Structure of the Escherichia coli 70S ribosome in complex with EF-Tu and Ile-tRNAIle(LAU) bound to the cognate AUA codon (Structure I) Deposited 2023-02-16	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 58-meric	Chain G 1–177（177 aa）	Not recorded	MG MAGNESIUM ION × 876 K POTASSIUM ION × 2 ILE ISOLEUCINE × 2 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 ZN ZINC ION × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.90 Å
8G7Q Structure of the Escherichia coli 70S ribosome in complex with EF-Tu and Ile-tRNAIle(LAU) bound to the near-cognate AUG codon (Structure II) Deposited 2023-02-16	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 58-meric	Chain G 1–177（177 aa）	Not recorded	PAR PAROMOMYCIN × 1 MG MAGNESIUM ION × 950 ILE ISOLEUCINE × 2 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 ZN ZINC ION × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.10 Å
8G7R Structure of the Escherichia coli 70S ribosome in complex with A-site tRNAIle(LAU) bound to the cognate AUA codon (Structure III) Deposited 2023-02-16	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric	Chain G 1–177（177 aa）	Not recorded	PAR PAROMOMYCIN × 1 MG MAGNESIUM ION × 734 K POTASSIUM ION × 2 ILE ISOLEUCINE × 1 ZN ZINC ION × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.80 Å
8G7S Structure of the Escherichia coli 70S ribosome in complex with P-site tRNAIle(LAU) bound to the cognate AUA codon (Structure IV) Deposited 2023-02-16	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric	Chain G 1–177（177 aa）	Not recorded	MG MAGNESIUM ION × 564 ILE ISOLEUCINE × 1 ZN ZINC ION × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.10 Å
8SYL Cryo-EM structure of the Escherichia coli 70S ribosome in complex with amikacin, mRNA, and A-, P-, and E-site tRNAs Deposited 2023-05-25	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric	Chain G 1–177（177 aa）	Not recorded	MG MAGNESIUM ION × 474 AKN (2S)-N-[(1R,2S,3S,4R,5S)-4-[(2R,3R,4S,5S,6R)-6-(aminomethyl)-3,4,5-tris(oxidanyl)oxan-2-yl]oxy-5-azanyl-2-[(2S,3R,4S,5S ,6R)-4-azanyl-6-(hydroxymethyl)-3,5-bis(oxidanyl)oxan-2-yl]oxy-3-oxidanyl-cyclohexyl]-4-azanyl-2-oxidanyl-butanamide × 2 PHE PHENYLALANINE × 1 ZN ZINC ION × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.90 Å
8UPO Escherichia coli transcription-translation coupled complex class A (TTC-A) containing RfaH bound to ops signal, mRNA with a 21 nt long spacer, and fMet-tRNAs in E-site and P-site of the ribosome Deposited 2023-10-23	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Other combination Heteromer；Protein × 58 PDB declaration: 66-meric	Chain p 1–177（177 aa）	Not recorded	MG MAGNESIUM ION × 1 ZN ZINC ION × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 5.50 Å
8UPR Escherichia coli transcription-translation coupled complex class A (TTC-A) containing RfaH bound to ops signal, mRNA with a 21 nt long spacer, and fMet-tRNAs in E-site and P-site of the ribosome Deposited 2023-10-23	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Other combination Heteromer；Protein × 59 PDB declaration: 67-meric	Chain p 1–177（177 aa）	Not recorded	MG MAGNESIUM ION × 1 ZN ZINC ION × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 5.30 Å
8UQL Escherichia coli transcription-translation coupled complex class B (TTC-B) containing RfaH in loaded state, mRNA with a 24 nt long spacer, and fMet-tRNAs in E-site and P-site of the ribosome Deposited 2023-10-24	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Other combination Heteromer；Protein × 59 PDB declaration: 67-meric	Chain p 1–177（177 aa）	Not recorded	MG MAGNESIUM ION × 1 ZN ZINC ION × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.20 Å
8UQM Escherichia coli transcription-translation coupled complex class B (TTC-B) containing RfaH in loaded state, NusA, mRNA with a 24 nt long spacer, and fMet-tRNAs in E-site and P-site of the ribosome Deposited 2023-10-24	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Other combination Heteromer；Protein × 60 PDB declaration: 68-meric	Chain p 1–177（177 aa）	Not recorded	MG MAGNESIUM ION × 1 ZN ZINC ION × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 5.30 Å
8UQP Escherichia coli transcription-translation coupled complex class B (TTC-B) containing RfaH bound to ops signal, mRNA with a 24 nt long spacer, and fMet-tRNAs in E-site and P-site of the ribosome Deposited 2023-10-24	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Other combination Heteromer；Protein × 59 PDB declaration: 67-meric	Chain p 1–177（177 aa）	Not recorded	MG MAGNESIUM ION × 1 ZN ZINC ION × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.80 Å
8UR0 Escherichia coli transcription-translation coupled complex class B (TTC-B) containing RfaH bound to ops signal, NusA, mRNA with a 24 nt long spacer, and fMet-tRNAs in E-site and P-site of the ribosome Deposited 2023-10-25	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Other combination Heteromer；Protein × 60 PDB declaration: 68-meric	Chain p 1–177（177 aa）	Not recorded	MG MAGNESIUM ION × 1 ZN ZINC ION × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.40 Å
8URH Escherichia coli transcription-translation coupled complex class B (TTC-B) containing RfaH bound to ops signal, mRNA with a 27 nt long spacer, and fMet-tRNAs in E-site and P-site of the ribosome Deposited 2023-10-26	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Other combination Heteromer；Protein × 59 PDB declaration: 67-meric	Chain p 1–177（177 aa）	Not recorded	MG MAGNESIUM ION × 1 ZN ZINC ION × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 5.70 Å
8URI Escherichia coli transcription-translation coupled complex class B (TTC-B) containing RfaH bound to ops signal, NusA, mRNA with a 27 nt long spacer, and fMet-tRNAs in E-site and P-site of the ribosome Deposited 2023-10-26	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Other combination Heteromer；Protein × 60 PDB declaration: 68-meric	Chain p 1–177（177 aa）	Not recorded	MG MAGNESIUM ION × 1 ZN ZINC ION × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 5.30 Å
8URM E. coli 70S ribosome with unmodified tRNAPro(GGG) bound to slippery P-site CCC-C codon and tRNAVal(UAC) in the A site Deposited 2023-10-26	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric	Chain F 1–177（177 aa）	Not recorded	MG MAGNESIUM ION × 395	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.00 Å
8URX Escherichia coli transcription-translation coupled complex class B (TTC-B) containing RfaH bound to ops signal, mRNA with a 30 nt long spacer, and fMet-tRNAs in E-site and P-site of the ribosome Deposited 2023-10-27	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Other combination Heteromer；Protein × 59 PDB declaration: 67-meric	Chain p 1–177（177 aa）	Not recorded	MG MAGNESIUM ION × 1 ZN ZINC ION × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 6.60 Å
8URY Escherichia coli transcription-translation coupled complex class B (TTC-B) containing RfaH bound to ops signal, NusA, mRNA with a 30 nt long spacer, and fMet-tRNA in E-site and P-site of the ribosome Deposited 2023-10-27	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Other combination Heteromer；Protein × 60 PDB declaration: 68-meric	Chain p 1–177（177 aa）	Not recorded	MG MAGNESIUM ION × 1 ZN ZINC ION × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.10 Å
8UTJ E. coli 70S ribosome with unmodified lys-tRNAPro(GGG) bound to slippery P-site CCC-C codon in the 0 frame Deposited 2023-10-31	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric	Chain F 1–177（177 aa）	Not recorded	MG MAGNESIUM ION × 530 LYS LYSINE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.50 Å
8UX8 E. coli 70S ribosome with unmodified Lys-tRNAPro(GGG) bound to slippery P-site CCC-C codon in the +1 mRNA reading frame Deposited 2023-11-09	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric	Chain F 1–177（177 aa）	Not recorded	MG MAGNESIUM ION × 395 LYS LYSINE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.60 Å
8UXB E. coli 70S ribosome with unmodified P/E-tRNAPro(GGG) bound to slippery P-site CCC-C codon Deposited 2023-11-09	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 53-meric	Chain F 1–177（177 aa）	Not recorded	MG MAGNESIUM ION × 287	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.90 Å
8UZ3 E. coli 70S ribosome with unmodified e*/E-tRNAPro(GGG) bound to slippery P-site CCC-C codon Deposited 2023-11-14	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 53-meric	Chain F 1–177（177 aa）	Not recorded	MG MAGNESIUM ION × 415	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.20 Å
8UZG E. coli 70S ribosome with unmodified e*/E-tRNAPro(GGG) bound to slippery P-site CCC-C codon Deposited 2023-11-15	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 53-meric	Chain F 1–177（177 aa）	Not recorded	MG MAGNESIUM ION × 398	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.70 Å
8V03 E. coli 70S ribosome with unmodified P/E-tRNAPro(GGG) bound to slippery P-site CCC-C codon Deposited 2023-11-16	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 53-meric	Chain F 1–177（177 aa）	Not recorded	MG MAGNESIUM ION × 281	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 4.00 Å
8VKV Escherichia coli transcription-translation coupled complex (TTC-B) containing mRNA with a 33 nt long spacer, ops signal, RfaH, NusA, and fMet-tRNAs in E-site and P-site Deposited 2024-01-10	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Other combination Heteromer；Protein × 60 PDB declaration: 68-meric	Chain p 1–177（177 aa）	Not recorded	MG MAGNESIUM ION × 1 ZN ZINC ION × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.60 Å
8VL1 Escherichia coli transcription-translation loosely coupled complex (TTC-LC) containing mRNA with a 36 nt long spacer, ops signal, RfaH, NusA, and fMet-tRNAs in E-site and P-site Deposited 2024-01-11	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Other combination Heteromer；Protein × 60 PDB declaration: 68-meric	Chain p 1–177（177 aa）	Not recorded	MG MAGNESIUM ION × 1 ZN ZINC ION × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 5.30 Å
8VOO Escherichia coli transcription-translation loosely coupled complex (TTC-LC) containing mRNA with a 39 nt long spacer, ops signal, RfaH, NusA, and fMet-tRNAs in E-site and P-site Deposited 2024-01-15	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Other combination Heteromer；Protein × 60 PDB declaration: 68-meric	Chain p 1–177（177 aa）	Not recorded	MG MAGNESIUM ION × 1 ZN ZINC ION × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.40 Å
8VOP Escherichia coli transcription-translation coupled complex (TTC-B) containing mRNA with a 36 nt long spacer, NusG, NusA, and fMet-tRNAs in E-site and P-site Deposited 2024-01-15	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Other combination Heteromer；Protein × 60 PDB declaration: 68-meric	Chain p 1–177（177 aa）	Not recorded	MG MAGNESIUM ION × 1 ZN ZINC ION × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 6.00 Å
8VOQ Escherichia coli transcription-translation loosely coupled complex (TTC-LC) containing mRNA with a 39 nt long spacer, NusG, NusA, and fMet-tRNAs in E-site and P-site Deposited 2024-01-15	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Other combination Heteromer；Protein × 60 PDB declaration: 68-meric	Chain p 1–177（177 aa）	Not recorded	MG MAGNESIUM ION × 1 ZN ZINC ION × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 9.30 Å
8VOR Escherichia coli transcription-translation loosely coupled complex (TTC-LC) containing mRNA with a 51 nt long spacer, NusG, NusA, and fMet-tRNAs in E-site and P-site Deposited 2024-01-15	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Other combination Heteromer；Protein × 60 PDB declaration: 68-meric	Chain p 1–177（177 aa）	Not recorded	MG MAGNESIUM ION × 1 ZN ZINC ION × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.60 Å
8VS9 Endogenous trans-translation complex with tmRNA*SmpB in the P site and alanyl-tRNA in the A site and deacyl-tRNA in the E site of E. coli 70S ribosome Deposited 2024-01-23	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 53 PDB declaration: 59-meric	Chain L06 1–177（177 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.90 Å
8VSA Endogenous trans-translation complex with tmRNA*SmpB in the P site and alanyl-tRNA in the A site of E. coli 70S ribosome Deposited 2024-01-23	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 53 PDB declaration: 58-meric	Chain L06 1–177（177 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.70 Å
8VUP E. coli 70S ribosome with unmodified tRNAPro(GGG) in the e*/E conformation on a slippery CCC-C codon Deposited 2024-01-29	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 53-meric	Chain F 2–177（176 aa）	Not recorded	MG MAGNESIUM ION × 239	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.40 Å
8VV4 E. coli 70S ribosome with unmodified Lys-tRNAPro(GGG) in the P/P conformation on a slippery CCC-C codon Deposited 2024-01-30	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 54-meric	Chain F 1–177（177 aa）	Not recorded	MG MAGNESIUM ION × 424 LYS LYSINE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.40 Å
8VWQ E. coli 70S ribosome with unmodified Lys-tRNAPro(GGG) in the P/P conformation on a slippery CCC-C codon and Elongation Factor P bound (uL1 in the closed conformation) Deposited 2024-02-02	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 56-meric	Chain F 1–177（177 aa）	Not recorded	MG MAGNESIUM ION × 586 LYS LYSINE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.10 Å
8W2N E. coli 70S ribosome with unmodified Lys-tRNAPro(GGG) in the P/P conformation on a slippery CCC-C codon and Elongation Factor P bound (uL1 in the open conformation) Deposited 2024-02-20	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric	Chain F 1–177（177 aa）	Not recorded	MG MAGNESIUM ION × 481	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.20 Å
9AX7 70S initiation complex (tRNA-fMet M1 + CUG start codon) Deposited 2024-03-06	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 54-meric	Chain g 1–177（177 aa）	Not recorded	ZN ZINC ION × 2 MG MAGNESIUM ION × 309 IAS BETA-L-ASPARTIC ACID × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.63 Å
9AX8 70S initiation complex (tRNA-fMet M1, initiation factor 2 + CUG start codon) Deposited 2024-03-06	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 53-meric	Chain H 2–177（176 aa）	Not recorded	MG MAGNESIUM ION × 287 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.60 Å
9B4Z E. coli 70S ribosome complex (N1-methylated 16S A1408 + arbekacin) Deposited 2024-03-21	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric	Chain BG 1–177（177 aa）	Not recorded	MG MAGNESIUM ION × 309 84G Arbekacin × 2 ZN ZINC ION × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.20 Å
9B50 E. coli 70S ribosome complex (unmethylated 16S A1408 + arbekacin) Deposited 2024-03-21	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 52-meric	Chain BG 1–177（177 aa）	Not recorded	MG MAGNESIUM ION × 309 84G Arbekacin × 2 ZN ZINC ION × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.70 Å
9B51 E. coli 70S ribosome complex (N1-methylated 16S A1408 + G418) Deposited 2024-03-21	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric	Chain BG 1–177（177 aa）	Not recorded	MG MAGNESIUM ION × 309 GET GENETICIN × 2 ZN ZINC ION × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.40 Å
9CG5 70S initiation complex (tRNA-fMet M1 + UUG start codon) Deposited 2024-06-28	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 54-meric	Chain g 1–177（177 aa）	Not recorded	ZN ZINC ION × 2 MG MAGNESIUM ION × 309 IAS BETA-L-ASPARTIC ACID × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.59 Å
9CG6 70S initiation complex (tRNA-fMet M1 + GUG start codon) Deposited 2024-06-28	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 54-meric	Chain g 1–177（177 aa）	Not recorded	ZN ZINC ION × 2 MG MAGNESIUM ION × 309 IAS BETA-L-ASPARTIC ACID × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.61 Å
9CG7 70S initiation complex (tRNA-fMet M1 + AUG start codon) Deposited 2024-06-28	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 54-meric	Chain g 1–177（177 aa）	Not recorded	ZN ZINC ION × 2 MG MAGNESIUM ION × 309 IAS BETA-L-ASPARTIC ACID × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.75 Å
9D89 E. coli 50S ribosomal subunit in complex with PrAMP rumicidin-2 (focused refinement) Deposited 2024-08-19	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 29 PDB declaration: 31-meric	Chain g 2–177（176 aa）	Not recorded	ZN ZINC ION × 1 MG MAGNESIUM ION × 204 K POTASSIUM ION × 93	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 1.95 Å
9NJF E. coli pre-elongation complex without an A-site tRNA with EQ2-YbiT in Non-hydrolytic 1/PtIM(a) conformation Deposited 2025-02-27	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 53 PDB declaration: 58-meric	Chain 6 2–177（176 aa）	Not recorded	MG MAGNESIUM ION × 124 ZN ZINC ION × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 NA SODIUM ION × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.40 Å
9NJV E. coli 70S initiation complex (bL33 absent) Deposited 2025-02-28	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 53-meric	Chain 6 2–177（176 aa）	Not recorded	MG MAGNESIUM ION × 152 FME N-FORMYLMETHIONINE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.10 Å
9NKL E. coli 70S initiation complex Deposited 2025-03-01	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 54-meric	Chain 6 2–177（176 aa）	Not recorded	MG MAGNESIUM ION × 161	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.30 Å
9NL5 E. coli pre-elongation complex without an A-site tRNA with EQ2-EttA in Hydrolytic 1 conformation Deposited 2025-03-02	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 56-meric	Chain 6 2–177（176 aa）	Not recorded	MG MAGNESIUM ION × 125 ZN ZINC ION × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 NA SODIUM ION × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.00 Å
9NL6 E. coli initiation complex with EQ2-YbiT in Non-hydrolytic 1/PtIM(a) conformation Deposited 2025-03-02	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric	Chain 6 2–177（176 aa）	Not recorded	MG MAGNESIUM ION × 252 FME N-FORMYLMETHIONINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 NA SODIUM ION × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.20 Å
9NL7 E. coli initiation complex with EQ2-YbiT in Non-hydrolytic 2/PtIM(b) conformation Deposited 2025-03-02	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 56-meric	Chain 6 2–177（176 aa）	Not recorded	MG MAGNESIUM ION × 250 FME N-FORMYLMETHIONINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 NA SODIUM ION × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.30 Å
9NLB E. coli initiation complex with EQ2-EttA in Hydrolytic 2 conformation Deposited 2025-03-03	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 56-meric	Chain 6 2–177（176 aa）	Not recorded	MG MAGNESIUM ION × 279 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 NA SODIUM ION × 2 FME N-FORMYLMETHIONINE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.20 Å
9NLE E. coli initiation complex with EQ2-EttA in Hydrolytic 1 conformation Deposited 2025-03-03	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 56-meric	Chain 6 2–177（176 aa）	Not recorded	MG MAGNESIUM ION × 298 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 NA SODIUM ION × 2 FME N-FORMYLMETHIONINE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.20 Å
9NLF E. coli initiation complex with EQ2-YbiT in Hydrolytic 2/PtIM(a) conformation Deposited 2025-03-03	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 56-meric	Chain 6 2–177（176 aa）	Not recorded	MG MAGNESIUM ION × 245 FME N-FORMYLMETHIONINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 NA SODIUM ION × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.20 Å
9NLH E.coli Initiation complex with YheS-EQ2 Deposited 2025-03-03	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric	Chain 6 2–177（176 aa）	Not recorded	MG MAGNESIUM ION × 263 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 NA SODIUM ION × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.10 Å
9NLJ E.coli Initiation complex with Uup-EQ2 Deposited 2025-03-03	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 56-meric	Chain 6 2–177（176 aa）	Not recorded	MG MAGNESIUM ION × 277 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 NA SODIUM ION × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.10 Å
9NLQ E. coli initiation complex with EQ2-YbiT in Hydrolytic 1/PtIM(a) conformation Deposited 2025-03-03	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 56-meric	Chain 6 2–177（176 aa）	Not recorded	MG MAGNESIUM ION × 241 FME N-FORMYLMETHIONINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 NA SODIUM ION × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.30 Å
9NLS E. coli initiation complex with EQ2-YbiT in Intermediate/PtIM(b) conformation Deposited 2025-03-03	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 56-meric	Chain 6 2–177（176 aa）	Not recorded	MG MAGNESIUM ION × 248 ZN ZINC ION × 1 FME N-FORMYLMETHIONINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 NA SODIUM ION × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.30 Å

68 other PDB entries and 68 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence

UniProt name	C3SR17_ECOLX
Isoform	—
PDB entities	13
Chains and sequence ranges	Author chain g； PDBConstruct 1–177； UniProt 1–177

50S ribosomal protein L9

OrganismNot specified

UniProt C3SFP2

State in the Current Structure

Assembly	Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Associated Components	Method and Experimental Conditions	Structure Quality
1	Protein–RNA Heteromer Protein × 30 RNA 2 PDB declaration: 32-meric（32） Consistent with all polymer counts	Chain h； UniProt 1–149	Not recorded	50S ribosomal protein L33 × 1 （A0A3S5YPG8） 50S ribosomal protein L34 × 1 （A0A8X6EVB8） 50S ribosomal protein L35 × 1 （A0A8S0FT45） 50S ribosomal protein L36 × 1 （A0A2X1PTA3） 50S ribosomal protein L31 × 1 （C3SIV2） Drosocin1 × 1 （B4MNS1） 23S ribosomal RNA × 1 5S ribosomal RNA × 1 50S ribosomal protein L2 × 1 （A0A7L6YWH1） 50S ribosomal protein L3 × 1 （C3SQU2） 50S ribosomal protein L4 × 1 （D8A1N0） 50S ribosomal protein L5 × 1 （D6IEK6） 50S ribosomal protein L6 × 1 （C3SR17） 50S ribosomal protein L13 × 1 （A0A656YGQ7） 50S ribosomal protein L14 × 1 （C3SQZ2） 50S ribosomal protein L15 × 1 （P02413） 50S ribosomal protein L16 × 1 （A0A0H3EMH6） 50S ribosomal protein L17 × 1 （A0A1X3JBW6） 50S ribosomal protein L18 × 1 （A0A514EKV0） 50S ribosomal protein L19 × 1 （A0A370V6W8） 50S ribosomal protein L20 × 1 （A0A378BCJ4） 50S ribosomal protein L21 × 1 （A0A0E2L2H1） 50S ribosomal protein L22 × 1 （A0A0F6B7N1） 50S ribosomal protein L23 × 1 （D7Y3D8） 50S ribosomal protein L24 × 1 （S1E9P3） 50S ribosomal protein L25 × 1 （P68919） 50S ribosomal protein L27 × 1 （C3SSG7） 50S ribosomal protein L28 × 1 （C3SME2） 50S ribosomal protein L29 × 1 （P0A7M6） 50S ribosomal protein L30 × 1 （C3SR32） 50S ribosomal protein L32 × 1 （A0A657HW13） ZN ZINC ION × 2 A2G 2-acetamido-2-deoxy-alpha-D-galactopyranose × 1 MG MAGNESIUM ION × 216 SPM SPERMINE × 1	ELECTRON MICROSCOPY cryo-EM buffer：pH 7.4 cryo-EM vitrification conditions：Cryogen ETHANE-PROPANE	Resolution 2.10 Å

Other States of the Same Protein in the Database

Other PDB	Difference from Current Entry 8ANA	Assembly / Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Non-polymers	Method and Experimental Conditions	Structure Quality
8AKN Cryo-EM structure of the proline-rich antimicrobial peptide drosocin bound to the terminating ribosome Deposited 2022-07-30	Different oligomeric state Different ligand/ion Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 57-meric	Chain h 1–149（149 aa）	Not recorded	ZN ZINC ION × 2 A2G 2-acetamido-2-deoxy-alpha-D-galactopyranose × 1 MG MAGNESIUM ION × 305 SPM SPERMINE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE-PROPANE	Resolution 2.30 Å
8AM9 Cryo-EM structure of the proline-rich antimicrobial peptide drosocin bound to the elongating ribosome Deposited 2022-08-03	Different oligomeric state Different ligand/ion Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric	Chain h 1–149（149 aa）	Not recorded	ZN ZINC ION × 2 MG MAGNESIUM ION × 293 SPM SPERMINE × 1 A2G 2-acetamido-2-deoxy-alpha-D-galactopyranose × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE-PROPANE	Resolution 2.80 Å
8URM E. coli 70S ribosome with unmodified tRNAPro(GGG) bound to slippery P-site CCC-C codon and tRNAVal(UAC) in the A site Deposited 2023-10-26	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric	Chain G 1–149（149 aa）	Not recorded	MG MAGNESIUM ION × 395	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.00 Å
8URY Escherichia coli transcription-translation coupled complex class B (TTC-B) containing RfaH bound to ops signal, NusA, mRNA with a 30 nt long spacer, and fMet-tRNA in E-site and P-site of the ribosome Deposited 2023-10-27	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Other combination Heteromer；Protein × 60 PDB declaration: 68-meric	Chain r 1–149（149 aa）	Not recorded	MG MAGNESIUM ION × 1 ZN ZINC ION × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.10 Å
8UTJ E. coli 70S ribosome with unmodified lys-tRNAPro(GGG) bound to slippery P-site CCC-C codon in the 0 frame Deposited 2023-10-31	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric	Chain G 1–149（149 aa）	Not recorded	MG MAGNESIUM ION × 530 LYS LYSINE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.50 Å
8UX8 E. coli 70S ribosome with unmodified Lys-tRNAPro(GGG) bound to slippery P-site CCC-C codon in the +1 mRNA reading frame Deposited 2023-11-09	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric	Chain G 1–149（149 aa）	Not recorded	MG MAGNESIUM ION × 395 LYS LYSINE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.60 Å
8V03 E. coli 70S ribosome with unmodified P/E-tRNAPro(GGG) bound to slippery P-site CCC-C codon Deposited 2023-11-16	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 53-meric	Chain G 1–149（149 aa）	Not recorded	MG MAGNESIUM ION × 281	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 4.00 Å
8VS9 Endogenous trans-translation complex with tmRNA*SmpB in the P site and alanyl-tRNA in the A site and deacyl-tRNA in the E site of E. coli 70S ribosome Deposited 2024-01-23	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 53 PDB declaration: 59-meric	Chain L09 1–149（149 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.90 Å
8VSA Endogenous trans-translation complex with tmRNA*SmpB in the P site and alanyl-tRNA in the A site of E. coli 70S ribosome Deposited 2024-01-23	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 53 PDB declaration: 58-meric	Chain L09 1–149（149 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.70 Å
8VUP E. coli 70S ribosome with unmodified tRNAPro(GGG) in the e*/E conformation on a slippery CCC-C codon Deposited 2024-01-29	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 53-meric	Chain G 1–149（149 aa）	Not recorded	MG MAGNESIUM ION × 239	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.40 Å
8VV4 E. coli 70S ribosome with unmodified Lys-tRNAPro(GGG) in the P/P conformation on a slippery CCC-C codon Deposited 2024-01-30	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 54-meric	Chain G 1–149（149 aa）	Not recorded	MG MAGNESIUM ION × 424 LYS LYSINE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.40 Å
8VWQ E. coli 70S ribosome with unmodified Lys-tRNAPro(GGG) in the P/P conformation on a slippery CCC-C codon and Elongation Factor P bound (uL1 in the closed conformation) Deposited 2024-02-02	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 56-meric	Chain G 1–149（149 aa）	Not recorded	MG MAGNESIUM ION × 586 LYS LYSINE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.10 Å
8W2N E. coli 70S ribosome with unmodified Lys-tRNAPro(GGG) in the P/P conformation on a slippery CCC-C codon and Elongation Factor P bound (uL1 in the open conformation) Deposited 2024-02-20	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric	Chain G 1–149（149 aa）	Not recorded	MG MAGNESIUM ION × 481	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.20 Å
9AX7 70S initiation complex (tRNA-fMet M1 + CUG start codon) Deposited 2024-03-06	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 54-meric	Chain h 1–149（149 aa）	Not recorded	ZN ZINC ION × 2 MG MAGNESIUM ION × 309 IAS BETA-L-ASPARTIC ACID × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.63 Å
9B4Z E. coli 70S ribosome complex (N1-methylated 16S A1408 + arbekacin) Deposited 2024-03-21	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric	Chain BH 1–149（149 aa）	Not recorded	MG MAGNESIUM ION × 309 84G Arbekacin × 2 ZN ZINC ION × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.20 Å
9B50 E. coli 70S ribosome complex (unmethylated 16S A1408 + arbekacin) Deposited 2024-03-21	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 52-meric	Chain BH 1–149（149 aa）	Not recorded	MG MAGNESIUM ION × 309 84G Arbekacin × 2 ZN ZINC ION × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.70 Å
9B51 E. coli 70S ribosome complex (N1-methylated 16S A1408 + G418) Deposited 2024-03-21	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric	Chain BH 1–149（149 aa）	Not recorded	MG MAGNESIUM ION × 309 GET GENETICIN × 2 ZN ZINC ION × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.40 Å
9CG5 70S initiation complex (tRNA-fMet M1 + UUG start codon) Deposited 2024-06-28	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 54-meric	Chain h 1–149（149 aa）	Not recorded	ZN ZINC ION × 2 MG MAGNESIUM ION × 309 IAS BETA-L-ASPARTIC ACID × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.59 Å
9CG6 70S initiation complex (tRNA-fMet M1 + GUG start codon) Deposited 2024-06-28	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 54-meric	Chain h 1–149（149 aa）	Not recorded	ZN ZINC ION × 2 MG MAGNESIUM ION × 309 IAS BETA-L-ASPARTIC ACID × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.61 Å
9CG7 70S initiation complex (tRNA-fMet M1 + AUG start codon) Deposited 2024-06-28	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 54-meric	Chain h 1–149（149 aa）	Not recorded	ZN ZINC ION × 2 MG MAGNESIUM ION × 309 IAS BETA-L-ASPARTIC ACID × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.75 Å

20 other PDB entries and 20 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence

UniProt name	C3SFP2_ECOLX
Isoform	—
PDB entities	14
Chains and sequence ranges	Author chain h； PDBConstruct 1–149； UniProt 1–149

50S ribosomal protein L13

OrganismNot specified

UniProt A0A656YGQ7

State in the Current Structure

Assembly	Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Associated Components	Method and Experimental Conditions	Structure Quality
1	Protein–RNA Heteromer Protein × 30 RNA 2 PDB declaration: 32-meric（32） Consistent with all polymer counts	Chain i； UniProt 1–142	Not recorded	50S ribosomal protein L33 × 1 （A0A3S5YPG8） 50S ribosomal protein L34 × 1 （A0A8X6EVB8） 50S ribosomal protein L35 × 1 （A0A8S0FT45） 50S ribosomal protein L36 × 1 （A0A2X1PTA3） 50S ribosomal protein L31 × 1 （C3SIV2） Drosocin1 × 1 （B4MNS1） 23S ribosomal RNA × 1 5S ribosomal RNA × 1 50S ribosomal protein L2 × 1 （A0A7L6YWH1） 50S ribosomal protein L3 × 1 （C3SQU2） 50S ribosomal protein L4 × 1 （D8A1N0） 50S ribosomal protein L5 × 1 （D6IEK6） 50S ribosomal protein L6 × 1 （C3SR17） 50S ribosomal protein L9 × 1 （C3SFP2） 50S ribosomal protein L14 × 1 （C3SQZ2） 50S ribosomal protein L15 × 1 （P02413） 50S ribosomal protein L16 × 1 （A0A0H3EMH6） 50S ribosomal protein L17 × 1 （A0A1X3JBW6） 50S ribosomal protein L18 × 1 （A0A514EKV0） 50S ribosomal protein L19 × 1 （A0A370V6W8） 50S ribosomal protein L20 × 1 （A0A378BCJ4） 50S ribosomal protein L21 × 1 （A0A0E2L2H1） 50S ribosomal protein L22 × 1 （A0A0F6B7N1） 50S ribosomal protein L23 × 1 （D7Y3D8） 50S ribosomal protein L24 × 1 （S1E9P3） 50S ribosomal protein L25 × 1 （P68919） 50S ribosomal protein L27 × 1 （C3SSG7） 50S ribosomal protein L28 × 1 （C3SME2） 50S ribosomal protein L29 × 1 （P0A7M6） 50S ribosomal protein L30 × 1 （C3SR32） 50S ribosomal protein L32 × 1 （A0A657HW13） ZN ZINC ION × 2 A2G 2-acetamido-2-deoxy-alpha-D-galactopyranose × 1 MG MAGNESIUM ION × 216 SPM SPERMINE × 1	ELECTRON MICROSCOPY cryo-EM buffer：pH 7.4 cryo-EM vitrification conditions：Cryogen ETHANE-PROPANE	Resolution 2.10 Å

Other States of the Same Protein in the Database

Other PDB	Difference from Current Entry 8ANA	Assembly / Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Non-polymers	Method and Experimental Conditions	Structure Quality
8AKN Cryo-EM structure of the proline-rich antimicrobial peptide drosocin bound to the terminating ribosome Deposited 2022-07-30	Different oligomeric state Different ligand/ion Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 57-meric	Chain i 1–142（142 aa）	Not recorded	ZN ZINC ION × 2 A2G 2-acetamido-2-deoxy-alpha-D-galactopyranose × 1 MG MAGNESIUM ION × 305 SPM SPERMINE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE-PROPANE	Resolution 2.30 Å
8AM9 Cryo-EM structure of the proline-rich antimicrobial peptide drosocin bound to the elongating ribosome Deposited 2022-08-03	Different oligomeric state Different ligand/ion Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric	Chain i 1–142（142 aa）	Not recorded	ZN ZINC ION × 2 MG MAGNESIUM ION × 293 SPM SPERMINE × 1 A2G 2-acetamido-2-deoxy-alpha-D-galactopyranose × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE-PROPANE	Resolution 2.80 Å

2 other PDB entries and 2 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence

UniProt name	A0A656YGQ7_SALNE
Isoform	—
PDB entities	15
Chains and sequence ranges	Author chain i； PDBConstruct 1–142； UniProt 1–142

50S ribosomal protein L14

OrganismNot specified

UniProt C3SQZ2

State in the Current Structure

Assembly	Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Associated Components	Method and Experimental Conditions	Structure Quality
1	Protein–RNA Heteromer Protein × 30 RNA 2 PDB declaration: 32-meric（32） Consistent with all polymer counts	Chain j； UniProt 1–123	Not recorded	50S ribosomal protein L33 × 1 （A0A3S5YPG8） 50S ribosomal protein L34 × 1 （A0A8X6EVB8） 50S ribosomal protein L35 × 1 （A0A8S0FT45） 50S ribosomal protein L36 × 1 （A0A2X1PTA3） 50S ribosomal protein L31 × 1 （C3SIV2） Drosocin1 × 1 （B4MNS1） 23S ribosomal RNA × 1 5S ribosomal RNA × 1 50S ribosomal protein L2 × 1 （A0A7L6YWH1） 50S ribosomal protein L3 × 1 （C3SQU2） 50S ribosomal protein L4 × 1 （D8A1N0） 50S ribosomal protein L5 × 1 （D6IEK6） 50S ribosomal protein L6 × 1 （C3SR17） 50S ribosomal protein L9 × 1 （C3SFP2） 50S ribosomal protein L13 × 1 （A0A656YGQ7） 50S ribosomal protein L15 × 1 （P02413） 50S ribosomal protein L16 × 1 （A0A0H3EMH6） 50S ribosomal protein L17 × 1 （A0A1X3JBW6） 50S ribosomal protein L18 × 1 （A0A514EKV0） 50S ribosomal protein L19 × 1 （A0A370V6W8） 50S ribosomal protein L20 × 1 （A0A378BCJ4） 50S ribosomal protein L21 × 1 （A0A0E2L2H1） 50S ribosomal protein L22 × 1 （A0A0F6B7N1） 50S ribosomal protein L23 × 1 （D7Y3D8） 50S ribosomal protein L24 × 1 （S1E9P3） 50S ribosomal protein L25 × 1 （P68919） 50S ribosomal protein L27 × 1 （C3SSG7） 50S ribosomal protein L28 × 1 （C3SME2） 50S ribosomal protein L29 × 1 （P0A7M6） 50S ribosomal protein L30 × 1 （C3SR32） 50S ribosomal protein L32 × 1 （A0A657HW13） ZN ZINC ION × 2 A2G 2-acetamido-2-deoxy-alpha-D-galactopyranose × 1 MG MAGNESIUM ION × 216 SPM SPERMINE × 1	ELECTRON MICROSCOPY cryo-EM buffer：pH 7.4 cryo-EM vitrification conditions：Cryogen ETHANE-PROPANE	Resolution 2.10 Å

Other States of the Same Protein in the Database

Other PDB	Difference from Current Entry 8ANA	Assembly / Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Non-polymers	Method and Experimental Conditions	Structure Quality
4V85 Crystal Structure of Release Factor RF3 Trapped in the GTP State on a Rotated Conformation of the Ribosome. Deposited 2011-06-13	Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 55 PDB declaration: 59-meric	Chain BO 1–123（123 aa）	Not recorded	MG MAGNESIUM ION × 493 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；295 K；Tris Ac PH.7.0, 25-35 mM KCL, 6.1% PEG 20000, 1% glycerol, 50mM sucrose, VAPOR DIFFUSION, SITTING DROP, temperature 295K	Resolution 3.20 Å R-free 0.250
7YLA Cryo-EM structure of 50S-HflX complex Deposited 2022-07-25	Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 30 PDB declaration: 32-meric	Chain S 1–122（122 aa）	Not recorded	GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 3 NA SODIUM ION × 1 ZN ZINC ION × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.52 Å
8AKN Cryo-EM structure of the proline-rich antimicrobial peptide drosocin bound to the terminating ribosome Deposited 2022-07-30	Different oligomeric state Different ligand/ion Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 57-meric	Chain j 1–123（123 aa）	Not recorded	ZN ZINC ION × 2 A2G 2-acetamido-2-deoxy-alpha-D-galactopyranose × 1 MG MAGNESIUM ION × 305 SPM SPERMINE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE-PROPANE	Resolution 2.30 Å
8AM9 Cryo-EM structure of the proline-rich antimicrobial peptide drosocin bound to the elongating ribosome Deposited 2022-08-03	Different oligomeric state Different ligand/ion Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric	Chain j 1–123（123 aa）	Not recorded	ZN ZINC ION × 2 MG MAGNESIUM ION × 293 SPM SPERMINE × 1 A2G 2-acetamido-2-deoxy-alpha-D-galactopyranose × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE-PROPANE	Resolution 2.80 Å
8G2U Time-resolved cryo-EM study of the 70S recycling by the HflX:control-apo-70S at 900ms Deposited 2023-02-06	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 51-meric	Chain K 2–123（122 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.00 Å
8G31 Time-resolved cryo-EM study of the 70S recycling by the HflX:2nd Intermediate Deposited 2023-02-06	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 52-meric	Chain K 2–123（122 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.20 Å
8G34 Time-resolved cryo-EM study of the 70S recycling by the HflX:1st intermediate Deposited 2023-02-06	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 52-meric	Chain K 2–123（122 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.20 Å
8G38 Time-resolved cryo-EM study of the 70S recycling by the HflX:3rd Intermediate Deposited 2023-02-07	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 52-meric	Chain K 2–123（122 aa）	Not recorded	GTP GUANOSINE-5'-TRIPHOSPHATE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.20 Å
8T5D Cryo-EM studies of the interplay between uS2 ribosomal protein and leaderless mRNA during bacterial translation initiation Deposited 2023-06-13	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 53-meric	Chain K 2–123（122 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.20 Å
8T5H Cryo-EM studies of the interplay between uS2 ribosomal protein and leaderless mRNA during bacterial translation initiation Deposited 2023-06-13	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 46 PDB declaration: 51-meric	Chain K 2–123（122 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.30 Å

10 other PDB entries and 10 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence

UniProt name	C3SQZ2_ECOLX
Isoform	—
PDB entities	16
Chains and sequence ranges	Author chain j； PDBConstruct 1–123； UniProt 1–123

50S ribosomal protein L15

OrganismNot specified

UniProt P02413

State in the Current Structure

Assembly	Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Associated Components	Method and Experimental Conditions	Structure Quality
1	Protein–RNA Heteromer Protein × 30 RNA 2 PDB declaration: 32-meric（32） Consistent with all polymer counts	Chain k； UniProt 1–144	Not recorded	50S ribosomal protein L33 × 1 （A0A3S5YPG8） 50S ribosomal protein L34 × 1 （A0A8X6EVB8） 50S ribosomal protein L35 × 1 （A0A8S0FT45） 50S ribosomal protein L36 × 1 （A0A2X1PTA3） 50S ribosomal protein L31 × 1 （C3SIV2） Drosocin1 × 1 （B4MNS1） 23S ribosomal RNA × 1 5S ribosomal RNA × 1 50S ribosomal protein L2 × 1 （A0A7L6YWH1） 50S ribosomal protein L3 × 1 （C3SQU2） 50S ribosomal protein L4 × 1 （D8A1N0） 50S ribosomal protein L5 × 1 （D6IEK6） 50S ribosomal protein L6 × 1 （C3SR17） 50S ribosomal protein L9 × 1 （C3SFP2） 50S ribosomal protein L13 × 1 （A0A656YGQ7） 50S ribosomal protein L14 × 1 （C3SQZ2） 50S ribosomal protein L16 × 1 （A0A0H3EMH6） 50S ribosomal protein L17 × 1 （A0A1X3JBW6） 50S ribosomal protein L18 × 1 （A0A514EKV0） 50S ribosomal protein L19 × 1 （A0A370V6W8） 50S ribosomal protein L20 × 1 （A0A378BCJ4） 50S ribosomal protein L21 × 1 （A0A0E2L2H1） 50S ribosomal protein L22 × 1 （A0A0F6B7N1） 50S ribosomal protein L23 × 1 （D7Y3D8） 50S ribosomal protein L24 × 1 （S1E9P3） 50S ribosomal protein L25 × 1 （P68919） 50S ribosomal protein L27 × 1 （C3SSG7） 50S ribosomal protein L28 × 1 （C3SME2） 50S ribosomal protein L29 × 1 （P0A7M6） 50S ribosomal protein L30 × 1 （C3SR32） 50S ribosomal protein L32 × 1 （A0A657HW13） ZN ZINC ION × 2 A2G 2-acetamido-2-deoxy-alpha-D-galactopyranose × 1 MG MAGNESIUM ION × 216 SPM SPERMINE × 1	ELECTRON MICROSCOPY cryo-EM buffer：pH 7.4 cryo-EM vitrification conditions：Cryogen ETHANE-PROPANE	Resolution 2.10 Å

Other States of the Same Protein in the Database

Other PDB	Difference from Current Entry 8ANA	Assembly / Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Non-polymers	Method and Experimental Conditions	Structure Quality
10QM E.coli 50S ribosomal subunit bound to compound 48a Deposited 2026-02-01	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 29 PDB declaration: 31-meric	Chain T 1–144（144 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY mmCIF provides none of the parsed conditions	Resolution 2.49 Å
11BQ Escherichia coli 70S ribosome containing an evolved 16S rRNA (EC-S3.5) Deposited 2026-02-16	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric	Chain k 1–144（144 aa）	Not recorded	ZN ZINC ION × 2 MG MAGNESIUM ION × 307 SPD SPERMIDINE × 14 SPM SPERMINE × 1 C CYTIDINE-5'-MONOPHOSPHATE × 1 G GUANOSINE-5'-MONOPHOSPHATE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.00 Å
11DG Chimeric Escherichia coli 70S ribosome containing an evolved Vibrio cholerae 16S rRNA (VC-S4.4) Deposited 2026-02-18	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric	Chain k 1–144（144 aa）	Not recorded	ZN ZINC ION × 2 MG MAGNESIUM ION × 272 SPD SPERMIDINE × 14 SPM SPERMINE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.00 Å
11EL Chimeric Escherichia coli 70S ribosome containing an evolved 16S rRNA from Vibrio cholerae (VC-ST) Deposited 2026-02-19	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric	Chain k 1–144（144 aa）	Not recorded	ZN ZINC ION × 2 MG MAGNESIUM ION × 308 SPD SPERMIDINE × 16 SPM SPERMINE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.20 Å
11FV Chimeric Escherichia coli 70S ribosome containing an evolved 16S rRNA from Pseudomonas aeruginosa (PA-S3.3) Deposited 2026-02-21	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 52-meric	Chain T 1–144（144 aa）	Not recorded	MG MAGNESIUM ION × 307	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.90 Å
11GG Chimeric Escherichia coli 70S ribosome containing an evolved 16S rRNA from Pseudomonas aeruginosa (PA-ST) Deposited 2026-02-22	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 52-meric	Chain T 1–144（144 aa）	Not recorded	MG MAGNESIUM ION × 307	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.02 Å
28UH E. coli 70S ribosome, trapped conformational ground state of SSU-h44 apical loop, with A/P- and P/E-site tRNA Deposited 2026-02-20	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric	Chain k 1–144（144 aa）	Not recorded	ZN ZINC ION × 2 MG MAGNESIUM ION × 274 K POTASSIUM ION × 65 SCM SPECTINOMYCIN × 1 VAL VALINE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.06 Å
28UI E. coli 70S ribosome, trapped conformational excited state of SSU-h44 apical loop, with A- and P-site tRNA Deposited 2026-02-20	Different oligomeric state Different ligand/ion Different experimental conditions	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric	Chain k 1–144（144 aa）	Not recorded	ZN ZINC ION × 2 SCM SPECTINOMYCIN × 1 MG MAGNESIUM ION × 270 VAL VALINE × 1 K POTASSIUM ION × 11	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.10 Å
28UJ E. coli 70S ribosome, trapped conformational excited state of SSU-h44 apical loop, with A/P- and P/E-site tRNA Deposited 2026-02-20	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric	Chain k 1–144（144 aa）	Not recorded	ZN ZINC ION × 2 MG MAGNESIUM ION × 251 SCM SPECTINOMYCIN × 1 K POTASSIUM ION × 47 VAL VALINE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.16 Å
28UK E. coli 70S ribosome, conformational ground state mutation in SSU-h41, with A- and P-site tRNA Deposited 2026-02-20	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric	Chain k 1–144（144 aa）	Not recorded	ZN ZINC ION × 2 MG MAGNESIUM ION × 227 K POTASSIUM ION × 74 SCM SPECTINOMYCIN × 1 VAL VALINE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.24 Å
28UL E. coli 70S ribosome, conformational ground state mutation in SSU-h41, with A/P- and P/E-site tRNA Deposited 2026-02-20	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric	Chain k 1–144（144 aa）	Not recorded	ZN ZINC ION × 2 MG MAGNESIUM ION × 212 K POTASSIUM ION × 77 SCM SPECTINOMYCIN × 1 VAL VALINE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.50 Å
28UM E. coli 70S ribosome, conformational excited state mutation in SSU-h41, with A- and P-site tRNA Deposited 2026-02-20	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric	Chain k 1–144（144 aa）	Not recorded	ZN ZINC ION × 2 MG MAGNESIUM ION × 238 K POTASSIUM ION × 109 SCM SPECTINOMYCIN × 1 VAL VALINE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.15 Å
28UN E. coli 70S ribosome, conformational excited state mutation in SSU-h41, with A/P- and P/E-site tRNA Deposited 2026-02-20	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric	Chain k 1–144（144 aa）	Not recorded	ZN ZINC ION × 2 MG MAGNESIUM ION × 183 K POTASSIUM ION × 65 SCM SPECTINOMYCIN × 1 VAL VALINE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.48 Å
2J28 MODEL OF E. COLI SRP BOUND TO 70S RNCS Deposited 2006-08-16	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 31 PDB declaration: 34-meric	Chain L 1–144（144 aa）	Not recorded	MG MAGNESIUM ION × 111	ELECTRON MICROSCOPY cryo-EM vitrification conditions Cryogen ETHANE	Resolution 9.50 Å
2RDO 50S subunit with EF-G(GDPNP) and RRF bound Deposited 2007-09-24	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 32 PDB declaration: 34-meric	Chain L 1–144（144 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer PolyMix；pH 7.5；PolyMix cryo-EM vitrification conditions Cryogen ETHANE；Rapid-freezing in liquid ethane by Vitrobot	Resolution 9.10 Å
30QZ E. coli 50S ribosome with peptidyl-tRNA and Hsp15 Deposited 2026-05-10	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 30 PDB declaration: 33-meric	Chain k 1–144（144 aa）	Not recorded	MG MAGNESIUM ION × 204 ZN ZINC ION × 1 ASP ASPARTIC ACID × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5；HEPES-polymix buffer (pH-7.5) cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.23 Å
30RA E. coli 70S ribosome with peptidyl-tRNA, mRNA and Hsp15 Deposited 2026-05-10	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric	Chain k 1–144（144 aa）	Not recorded	ZN ZINC ION × 2 MG MAGNESIUM ION × 281 ASP ASPARTIC ACID × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5；HEPES-polymix buffer (pH-7.5) cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.78 Å
30RB E. coli 70S ribosome with peptidyl-tRNA and Hsp15 Deposited 2026-05-10	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric	Chain k 1–144（144 aa）	Not recorded	ZN ZINC ION × 1 MG MAGNESIUM ION × 210 K POTASSIUM ION × 2 ASP ASPARTIC ACID × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5；HEPES-polymix buffer (pH-7.5) cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.92 Å
3BBX The Hsp15 protein fitted into the low resolution Cryo-EM map of the 50S.nc-tRNA.Hsp15 complex Deposited 2007-11-11	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 28 PDB declaration: 30-meric	Chain L 1–144（144 aa）	Not recorded	MG MAGNESIUM ION × 110	ELECTRON MICROSCOPY cryo-EM vitrification conditions Cryogen ETHANE	Resolution 10.00 Å
3IY9 Leishmania Tarentolae Mitochondrial Large Ribosomal Subunit Model Deposited 2009-04-20	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 19 PDB declaration: eicosameric	Chain L 1–144（144 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer 50 mM Tris HCL, pH 7.5, 100mM KCL, 10mM MgCl2, 3mM DTT, 0.1mM EDTA, 0.05% dodecyl maltoside；pH 7.5；50 mM Tris HCL, pH 7.5, 100mM KCL, 10mM MgCl2, 3mM DTT, 0.1mM EDTA, 0.05% dodecyl maltoside cryo-EM vitrification conditions Cryogen ETHANE	Resolution 14.10 Å
3J5L Structure of the E. coli 50S subunit with ErmBL nascent chain Deposited 2013-10-23	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 30 PDB declaration: 34-meric	Chain L 2–144（143 aa）	Not recorded	UNL UNKNOWN LIGAND × 1 ERY ERYTHROMYCIN A × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE；Plunged into liquid ethane (FEI VITROBOT MARK IV)	Resolution 6.60 Å
3J7Z Structure of the E. coli 50S subunit with ErmCL nascent chain Deposited 2014-08-27	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 32 PDB declaration: 35-meric	Chain L 1–144（144 aa）	Not recorded	ERY ERYTHROMYCIN A × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE；Plunged into liquid ethane (FEI VITROBOT MARK IV).	Resolution 3.90 Å
3J8G Electron cryo-microscopy structure of EngA bound with the 50S ribosomal subunit Deposited 2014-10-24	Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 30 PDB declaration: 32-meric	Chain L 1–144（144 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 5.00 Å
3J9Y Cryo-EM structure of tetracycline resistance protein TetM bound to a translating E.coli ribosome Deposited 2015-03-23	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 53 PDB declaration: 58-meric	Chain L 1–144（144 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer 50 mM HEPES-KOH, 100 mM KOAc, 25 mM Mg(OAc)2, 6 mM b-mercaptoethanol；pH 7.4；50 mM HEPES-KOH, 100 mM KOAc, 25 mM Mg(OAc)2, 6 mM b-mercaptoethanol cryo-EM vitrification conditions Cryogen ETHANE；Plunged into liquid ethane (FEI VITROBOT MARK IV)	Resolution 3.90 Å
3J9Z Activation of GTP Hydrolysis in mRNA-tRNA Translocation by Elongation Factor G Deposited 2015-03-27	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 53 PDB declaration: 58-meric	Chain LH 1–144（144 aa）	Not recorded	GTP GUANOSINE-5'-TRIPHOSPHATE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions 93 K；Cryogen ETHANE；Plunged into liquid ethane (FEI VITROBOT MARK IV)	Resolution 3.60 Å
3JA1 Activation of GTP Hydrolysis in mRNA-tRNA Translocation by Elongation Factor G Deposited 2015-03-30	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 53 PDB declaration: 58-meric	Chain LN 1–144（144 aa）	Not recorded	GTP GUANOSINE-5'-TRIPHOSPHATE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions 93 K；Cryogen ETHANE；Plunged into liquid ethane (FEI VITROBOT MARK IV)	Resolution 3.60 Å
3JBU Mechanisms of Ribosome Stalling by SecM at Multiple Elongation Steps Deposited 2015-10-16	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 54-meric	Chain l 1–144（144 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer 20mM HEPES, 50mM KOAc, 6mM Mg(OAc)2, 1mM DTT, 500 ug/ml chloramphenicol, 0.05% Nikkol, 0.5% pill/ml Complete EDTA-free Protease inhibitor cocktail, 0.1U/ml RNasin and 125mM sucrose；pH 7；20mM HEPES, 50mM KOAc, 6mM Mg(OAc)2, 1mM DTT, 500 ug/ml chloramphenicol, 0.05% Nikkol, 0.5% pill/ml Complete EDTA-free Protease inhibitor cocktail, 0.1U/ml RNasin and 125mM sucrose cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.64 Å
3JBV Mechanisms of Ribosome Stalling by SecM at Multiple Elongation Steps Deposited 2015-10-16	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric	Chain l 1–144（144 aa）	Not recorded	CLM CHLORAMPHENICOL × 1	ELECTRON MICROSCOPY cryo-EM buffer 20mM HEPES, 50mM KOAc, 6mM Mg(OAc)2, 1mM DTT, 500 ug/ml chloramphenicol,0.05% Nikkol,0.5% pill/ml Complete EDTA-free Protease inhibitor cocktail,0.1 U/ml RNasin and 125mM sucrose；pH 7；20mM HEPES, 50mM KOAc, 6mM Mg(OAc)2, 1mM DTT, 500 ug/ml chloramphenicol,0.05% Nikkol,0.5% pill/ml Complete EDTA-free Protease inhibitor cocktail,0.1 U/ml RNasin and 125mM sucrose cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.32 Å
3JCD Structure of Escherichia coli EF4 in posttranslocational ribosomes (Post EF4) Deposited 2015-12-01	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 57-meric	Chain L 1–144（144 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.70 Å
3JCE Structure of Escherichia coli EF4 in pretranslocational ribosomes (Pre EF4) Deposited 2015-12-01	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 58-meric	Chain L 1–144（144 aa）	Not recorded	PHE PHENYLALANINE × 1 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1	ELECTRON MICROSCOPY cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.20 Å
3JCJ Structures of ribosome-bound initiation factor 2 reveal the mechanism of subunit association Deposited 2015-12-18	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 56-meric	Chain K 1–144（144 aa）	Not recorded	MG MAGNESIUM ION × 195 ZN ZINC ION × 1 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 FME N-FORMYLMETHIONINE × 1	ELECTRON MICROSCOPY cryo-EM buffer 20 mM HEPES-KOH, pH 7.5, 15 mM magnesium acetate, 150 mM potassium acetate, 4 mM 2-mercapthoethanol, 2 mM spermidine, 0.05 mM spermine；pH 7.5；20 mM HEPES-KOH, pH 7.5, 15 mM magnesium acetate, 150 mM potassium acetate, 4 mM 2-mercapthoethanol, 2 mM spermidine, 0.05 mM spermine cryo-EM vitrification conditions Blot for 2-4 seconds before plunging；Cryogen ETHANE；Blot for 2-4 seconds before plunging into liquid ethane (FEI VITROBOT MARK I).	Resolution 3.70 Å
3JCN Structures of ribosome-bound initiation factor 2 reveal the mechanism of subunit association: Initiation Complex I Deposited 2016-01-04	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric	Chain L 1–144（144 aa）	Not recorded	GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 FME N-FORMYLMETHIONINE × 1	ELECTRON MICROSCOPY cryo-EM buffer 20 mM HEPES-KOH, pH 7.5, 15 mM magnesium acetate, 150 mM potassium acetate, 4 mM 2-mercapthoethanol, 2 mM spermidine, 0.05 mM spermine；pH 7.5；20 mM HEPES-KOH, pH 7.5, 15 mM magnesium acetate, 150 mM potassium acetate, 4 mM 2-mercapthoethanol, 2 mM spermidine, 0.05 mM spermine cryo-EM vitrification conditions Blot for 2-4 seconds before plunging；Cryogen ETHANE；Blot for 2-4 seconds before plunging into liquid ethane (FEI VITROBOT MARK I).	Resolution 4.60 Å
4CSU Cryo-EM structures of the 50S ribosome subunit bound with ObgE Deposited 2014-03-10	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 31 PDB declaration: 33-meric	Chain L 2–144（143 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer 20MM TRIS-HCL, 100MM NH4CL,10MM MGCL2；pH 7.5；20MM TRIS-HCL, 100MM NH4CL,10MM MGCL2 cryo-EM vitrification conditions Cryogen ETHANE；LIQUID ETHANE	Resolution 5.50 Å
4U1U Crystal structure of the E. coli ribosome bound to quinupristin. Deposited 2014-07-16	Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 54-meric	Chain BL 2–144（143 aa）	Not recorded	MG MAGNESIUM ION × 271 ZN ZINC ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions EVAPORATION；pH 6.5；291 K；PEG8k, MPD	Resolution 2.95 Å R-free 0.282
4U1U Crystal structure of the E. coli ribosome bound to quinupristin. Deposited 2014-07-16	Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 53-meric	Chain DL 2–144（143 aa）	Not recorded	MG MAGNESIUM ION × 227 ZN ZINC ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions EVAPORATION；pH 6.5；291 K；PEG8k, MPD	Resolution 2.95 Å R-free 0.282
4U1V Crystal structure of the E. coli ribosome bound to linopristin. Deposited 2014-07-16	Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 54-meric	Chain BL 2–144（143 aa）	Not recorded	MG MAGNESIUM ION × 271 ZN ZINC ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions MICROBATCH；pH 6.5；291 K；PEG8k, MPD	Resolution 3.00 Å R-free 0.274
4U1V Crystal structure of the E. coli ribosome bound to linopristin. Deposited 2014-07-16	Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 53-meric	Chain DL 2–144（143 aa）	Not recorded	MG MAGNESIUM ION × 227 ZN ZINC ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions MICROBATCH；pH 6.5；291 K；PEG8k, MPD	Resolution 3.00 Å R-free 0.274
4U20 Crystal structure of the E. coli ribosome bound to flopristin. Deposited 2014-07-16	Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 53-meric	Chain BL 2–144（143 aa）	Not recorded	MG MAGNESIUM ION × 271 VIF Flopristin × 1 ZN ZINC ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions MICROBATCH；pH 6.5；291 K；PEG8k, MPD	Resolution 2.90 Å R-free 0.279
4U20 Crystal structure of the E. coli ribosome bound to flopristin. Deposited 2014-07-16	Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 52-meric	Chain DL 2–144（143 aa）	Not recorded	MG MAGNESIUM ION × 227 VIF Flopristin × 1 ZN ZINC ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions MICROBATCH；pH 6.5；291 K；PEG8k, MPD	Resolution 2.90 Å R-free 0.279
4U24 Crystal structure of the E. coli ribosome bound to dalfopristin. Deposited 2014-07-16	Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 53-meric	Chain BL 2–144（143 aa）	Not recorded	MG MAGNESIUM ION × 271 DOL 5-(2-DIETHYLAMINO-ETHANESULFONYL)-21-HYDROXY-10-ISOPROPYL-11,19-DIMETHYL-9,26-DIOXA-3,15,28-TRIAZA-TRICYCLO[23.2.1.00,255]OCTACOSA-1(27),12,17,19,25(28)-PENTAENE-2,8,14,23-TETRAONE × 1 ZN ZINC ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions MICROBATCH；pH 6.5；291 K；PEG8k, MPD	Resolution 2.90 Å R-free 0.264
4U24 Crystal structure of the E. coli ribosome bound to dalfopristin. Deposited 2014-07-16	Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 52-meric	Chain DL 2–144（143 aa）	Not recorded	MG MAGNESIUM ION × 226 DOL 5-(2-DIETHYLAMINO-ETHANESULFONYL)-21-HYDROXY-10-ISOPROPYL-11,19-DIMETHYL-9,26-DIOXA-3,15,28-TRIAZA-TRICYCLO[23.2.1.00,255]OCTACOSA-1(27),12,17,19,25(28)-PENTAENE-2,8,14,23-TETRAONE × 1 ZN ZINC ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions MICROBATCH；pH 6.5；291 K；PEG8k, MPD	Resolution 2.90 Å R-free 0.264
4U25 Crystal structure of the E. coli ribosome bound to virginiamycin M1. Deposited 2014-07-16	Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 53-meric	Chain BL 2–144（143 aa）	Not recorded	MG MAGNESIUM ION × 271 VIR VIRGINIAMYCIN M1 × 1 ZN ZINC ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions MICROBATCH；pH 6.5；291 K；PEG8k, MPD	Resolution 2.90 Å R-free 0.265
4U25 Crystal structure of the E. coli ribosome bound to virginiamycin M1. Deposited 2014-07-16	Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 52-meric	Chain DL 2–144（143 aa）	Not recorded	MG MAGNESIUM ION × 227 VIR VIRGINIAMYCIN M1 × 1 ZN ZINC ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions MICROBATCH；pH 6.5；291 K；PEG8k, MPD	Resolution 2.90 Å R-free 0.265
4U26 Crystal structure of the E. coli ribosome bound to dalfopristin and quinupristin. Deposited 2014-07-16	Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 54-meric	Chain BL 2–144（143 aa）	Not recorded	MG MAGNESIUM ION × 271 DOL 5-(2-DIETHYLAMINO-ETHANESULFONYL)-21-HYDROXY-10-ISOPROPYL-11,19-DIMETHYL-9,26-DIOXA-3,15,28-TRIAZA-TRICYCLO[23.2.1.00,255]OCTACOSA-1(27),12,17,19,25(28)-PENTAENE-2,8,14,23-TETRAONE × 1 ZN ZINC ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions MICROBATCH；pH 6.5；291 K；PEG8k, MPD	Resolution 2.80 Å R-free 0.271
4U26 Crystal structure of the E. coli ribosome bound to dalfopristin and quinupristin. Deposited 2014-07-16	Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 53-meric	Chain DL 2–144（143 aa）	Not recorded	MG MAGNESIUM ION × 227 DOL 5-(2-DIETHYLAMINO-ETHANESULFONYL)-21-HYDROXY-10-ISOPROPYL-11,19-DIMETHYL-9,26-DIOXA-3,15,28-TRIAZA-TRICYCLO[23.2.1.00,255]OCTACOSA-1(27),12,17,19,25(28)-PENTAENE-2,8,14,23-TETRAONE × 1 ZN ZINC ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions MICROBATCH；pH 6.5；291 K；PEG8k, MPD	Resolution 2.80 Å R-free 0.271
4U27 Crystal structure of the E. coli ribosome bound to flopristin and linopristin. Deposited 2014-07-16	Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 54-meric	Chain BL 2–144（143 aa）	Not recorded	MG MAGNESIUM ION × 271 VIF Flopristin × 1 ZN ZINC ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions MICROBATCH；pH 6.5；291 K；PEG8k, MPD	Resolution 2.80 Å R-free 0.260
4U27 Crystal structure of the E. coli ribosome bound to flopristin and linopristin. Deposited 2014-07-16	Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 53-meric	Chain DL 2–144（143 aa）	Not recorded	MG MAGNESIUM ION × 227 VIF Flopristin × 1 ZN ZINC ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions MICROBATCH；pH 6.5；291 K；PEG8k, MPD	Resolution 2.80 Å R-free 0.260
4UY8 Molecular basis for the ribosome functioning as a L-tryptophan sensor - Cryo-EM structure of a TnaC stalled E.coli ribosome Deposited 2014-08-29	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 32 PDB declaration: 35-meric	Chain L 2–144（143 aa）	Not recorded	MG MAGNESIUM ION × 143 ZN ZINC ION × 1 TRP TRYPTOPHAN × 2	ELECTRON MICROSCOPY cryo-EM vitrification conditions Cryogen ETHANE；VITRIFICATION 1 --CRYOGEN- ETHANE,INSTRUMENT- FEI VITROBOT MARK IV	Resolution 3.80 Å
4V47 Real space refined coordinates of the 30S and 50S subunits fitted into the low resolution cryo-EM map of the EF-G.GTP state of E. coli 70S ribosome Deposited 2003-05-06	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 43 PDB declaration: 46-meric	Chain AJ 1–144（144 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer Tris；pH 7.5；Tris cryo-EM vitrification conditions Rapid-freezing in liquid ethane	Resolution 12.30 Å
4V48 Real space refined coordinates of the 30S and 50S subunits fitted into the low resolution cryo-EM map of the initiation-like state of E. coli 70S ribosome Deposited 2003-05-06	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 44 PDB declaration: 48-meric	Chain AJ 1–144（144 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer hepes；pH 7.5；hepes cryo-EM vitrification conditions Rapid-freezing in liquid ethane	Resolution 11.50 Å
4V4H Crystal structure of the bacterial ribosome from Escherichia coli in complex with the antibiotic kasugamyin at 3.5A resolution. Deposited 2006-08-04	Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 52-meric	Chain BL 1–144（144 aa）	Not recorded	KSG (1S,2R,3S,4R,5S,6S)-2,3,4,5,6-PENTAHYDROXYCYCLOHEXYL 2-AMINO-4-{[CARBOXY(IMINO)METHYL]AMINO}-2,3,4,6-TETRADEOXY-ALPHA-D-ARABINO-HEXOPYRANOSIDE × 1 MG MAGNESIUM ION × 170	X-RAY DIFFRACTION X-ray crystallization conditions pH 7.5；MPD, PEG 8000, MGCL2, NH4CL, SPERMINE, SPERMIDINE, TRIS-HCL, EDTA, PH 7.5, BATCH, TEMPERATURE 277K, pH 7.50	Resolution 3.46 Å R-free 0.331
4V4H Crystal structure of the bacterial ribosome from Escherichia coli in complex with the antibiotic kasugamyin at 3.5A resolution. Deposited 2006-08-04	Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 52-meric	Chain DL 1–144（144 aa）	Not recorded	KSG (1S,2R,3S,4R,5S,6S)-2,3,4,5,6-PENTAHYDROXYCYCLOHEXYL 2-AMINO-4-{[CARBOXY(IMINO)METHYL]AMINO}-2,3,4,6-TETRADEOXY-ALPHA-D-ARABINO-HEXOPYRANOSIDE × 1 MG MAGNESIUM ION × 173	X-RAY DIFFRACTION X-ray crystallization conditions pH 7.5；MPD, PEG 8000, MGCL2, NH4CL, SPERMINE, SPERMIDINE, TRIS-HCL, EDTA, PH 7.5, BATCH, TEMPERATURE 277K, pH 7.50	Resolution 3.46 Å R-free 0.331
4V4Q Crystal structure of the bacterial ribosome from Escherichia coli at 3.5 A resolution. Deposited 2005-08-30	Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 52-meric	Chain BL 1–144（144 aa）	Not recorded	MG MAGNESIUM ION × 170	X-RAY DIFFRACTION X-ray crystallization conditions batch；pH 7.5；277 K；MPD, PEG 8000, MgCl2, NH4Cl, spermine, spermidine, TRIS, EDTA, pH 7.5, batch, temperature 277K	Resolution 3.46 Å R-free 0.331
4V4Q Crystal structure of the bacterial ribosome from Escherichia coli at 3.5 A resolution. Deposited 2005-08-30	Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 52-meric	Chain DL 1–144（144 aa）	Not recorded	MG MAGNESIUM ION × 173	X-RAY DIFFRACTION X-ray crystallization conditions batch；pH 7.5；277 K；MPD, PEG 8000, MgCl2, NH4Cl, spermine, spermidine, TRIS, EDTA, pH 7.5, batch, temperature 277K	Resolution 3.46 Å R-free 0.331
4V4V Structure of a pre-translocational E. coli ribosome obtained by fitting atomic models for RNA and protein components into cryo-EM map EMD-1056 Deposited 2006-05-09	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 46 PDB declaration: 52-meric	Chain BJ 4–143（140 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE；rapid-freezing in liquid ethane	Resolution 15.00 Å
4V4W Structure of a SecM-stalled E. coli ribosome complex obtained by fitting atomic models for RNA and protein components into cryo-EM map EMD-1143 Deposited 2006-05-09	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 46 PDB declaration: 52-meric	Chain BJ 4–143（140 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE；rapid-freezing in liquid ethane	Resolution 15.00 Å
4V50 Crystal Structure of Ribosome with messenger RNA and the Anticodon stem-loop of P-site tRNA. Deposited 2006-08-16	Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 54-meric	Chain BL 1–144（144 aa）	Not recorded	MG MAGNESIUM ION × 181 ZN ZINC ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions batch；pH 7.5；277 K；MPD, PEG 8000, MGCL2, NH4CL, SPERMINE, SPERMIDINE, TRIS, EDTA, pH 7.5, batch, temperature 277K	Resolution 3.22 Å R-free 0.320
4V50 Crystal Structure of Ribosome with messenger RNA and the Anticodon stem-loop of P-site tRNA. Deposited 2006-08-16	Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 54-meric	Chain DL 1–144（144 aa）	Not recorded	MG MAGNESIUM ION × 177 ZN ZINC ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions batch；pH 7.5；277 K；MPD, PEG 8000, MGCL2, NH4CL, SPERMINE, SPERMIDINE, TRIS, EDTA, pH 7.5, batch, temperature 277K	Resolution 3.22 Å R-free 0.320
4V52 Crystal structure of the bacterial ribosome from Escherichia coli in complex with neomycin. Deposited 2007-06-15	Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 52-meric	Chain BL 1–144（144 aa）	Not recorded	NMY NEOMYCIN × 2 MG MAGNESIUM ION × 170 ZN ZINC ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions batch；pH 7.5；277 K；MPD, PEG 8000, MgCl2, NH4Cl, spermine, spermidine, TRIS, EDTA, pH 7.5, batch, temperature 277K	Resolution 3.21 Å R-free 0.309
4V52 Crystal structure of the bacterial ribosome from Escherichia coli in complex with neomycin. Deposited 2007-06-15	Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 52-meric	Chain DL 1–144（144 aa）	Not recorded	NMY NEOMYCIN × 2 MG MAGNESIUM ION × 173 ZN ZINC ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions batch；pH 7.5；277 K；MPD, PEG 8000, MgCl2, NH4Cl, spermine, spermidine, TRIS, EDTA, pH 7.5, batch, temperature 277K	Resolution 3.21 Å R-free 0.309
4V53 Crystal structure of the bacterial ribosome from Escherichia coli in complex with gentamicin. Deposited 2007-06-16	Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 52-meric	Chain BL 1–144（144 aa）	Not recorded	MG MAGNESIUM ION × 170 LLL (2R,3R,4R,5R)-2-((1S,2S,3R,4S,6R)-4,6-DIAMINO-3-((2R,3R,6S)-3-AMINO-6-(AMINOMETHYL)-TETRAHYDRO-2H-PYRAN-2-YLOXY)-2-HYDR OXYCYCLOHEXYLOXY)-5-METHYL-4-(METHYLAMINO)-TETRAHYDRO-2H-PYRAN-3,5-DIOL × 4 ZN ZINC ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions batch；pH 7.5；277 K；MPD, PEG 8000, MgCl2, NH4Cl, spermine, spermidine, TRIS, EDTA, pH 7.5, batch, temperature 277K	Resolution 3.54 Å R-free 0.320
4V53 Crystal structure of the bacterial ribosome from Escherichia coli in complex with gentamicin. Deposited 2007-06-16	Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 52-meric	Chain DL 1–144（144 aa）	Not recorded	MG MAGNESIUM ION × 173 LLL (2R,3R,4R,5R)-2-((1S,2S,3R,4S,6R)-4,6-DIAMINO-3-((2R,3R,6S)-3-AMINO-6-(AMINOMETHYL)-TETRAHYDRO-2H-PYRAN-2-YLOXY)-2-HYDR OXYCYCLOHEXYLOXY)-5-METHYL-4-(METHYLAMINO)-TETRAHYDRO-2H-PYRAN-3,5-DIOL × 4 ZN ZINC ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions batch；pH 7.5；277 K；MPD, PEG 8000, MgCl2, NH4Cl, spermine, spermidine, TRIS, EDTA, pH 7.5, batch, temperature 277K	Resolution 3.54 Å R-free 0.320
4V54 Crystal structure of the bacterial ribosome from Escherichia coli in complex with ribosome recycling factor (RRF). Deposited 2007-06-16	Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 53-meric	Chain BL 1–144（144 aa）	Not recorded	MG MAGNESIUM ION × 170 ZN ZINC ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions batch；pH 7.5；277 K；MPD, PEG 8000, MgCl2, NH4Cl, spermine, spermidine, TRIS, EDTA, pH 7.5, batch, temperature 277K	Resolution 3.30 Å R-free 0.304
4V54 Crystal structure of the bacterial ribosome from Escherichia coli in complex with ribosome recycling factor (RRF). Deposited 2007-06-16	Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 53-meric	Chain DL 1–144（144 aa）	Not recorded	MG MAGNESIUM ION × 173 ZN ZINC ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions batch；pH 7.5；277 K；MPD, PEG 8000, MgCl2, NH4Cl, spermine, spermidine, TRIS, EDTA, pH 7.5, batch, temperature 277K	Resolution 3.30 Å R-free 0.304
4V55 Crystal structure of the bacterial ribosome from Escherichia coli in complex with gentamicin and ribosome recycling factor (RRF). Deposited 2007-06-17	Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 53-meric	Chain BL 1–144（144 aa）	Not recorded	MG MAGNESIUM ION × 170 LLL (2R,3R,4R,5R)-2-((1S,2S,3R,4S,6R)-4,6-DIAMINO-3-((2R,3R,6S)-3-AMINO-6-(AMINOMETHYL)-TETRAHYDRO-2H-PYRAN-2-YLOXY)-2-HYDR OXYCYCLOHEXYLOXY)-5-METHYL-4-(METHYLAMINO)-TETRAHYDRO-2H-PYRAN-3,5-DIOL × 2 ZN ZINC ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions batch；pH 7.5；277 K；MPD, PEG 8000, MgCl2, NH4Cl, spermine, spermidine, TRIS, EDTA, pH 7.5, batch, temperature 277K	Resolution 4.00 Å R-free 0.305
4V55 Crystal structure of the bacterial ribosome from Escherichia coli in complex with gentamicin and ribosome recycling factor (RRF). Deposited 2007-06-17	Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 53-meric	Chain DL 1–144（144 aa）	Not recorded	MG MAGNESIUM ION × 173 LLL (2R,3R,4R,5R)-2-((1S,2S,3R,4S,6R)-4,6-DIAMINO-3-((2R,3R,6S)-3-AMINO-6-(AMINOMETHYL)-TETRAHYDRO-2H-PYRAN-2-YLOXY)-2-HYDR OXYCYCLOHEXYLOXY)-5-METHYL-4-(METHYLAMINO)-TETRAHYDRO-2H-PYRAN-3,5-DIOL × 2 ZN ZINC ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions batch；pH 7.5；277 K；MPD, PEG 8000, MgCl2, NH4Cl, spermine, spermidine, TRIS, EDTA, pH 7.5, batch, temperature 277K	Resolution 4.00 Å R-free 0.305
4V56 Crystal structure of the bacterial ribosome from Escherichia coli in complex with spectinomycin. Deposited 2007-07-21	Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 52-meric	Chain BL 1–144（144 aa）	Not recorded	MG MAGNESIUM ION × 170 SCM SPECTINOMYCIN × 1 ZN ZINC ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions batch；pH 7.5；277 K；MPD, PEG 8000, MgCl2, NH4Cl, spermine, spermidine, TRIS, EDTA, pH 7.5, batch, temperature 277K	Resolution 3.93 Å R-free 0.311
4V56 Crystal structure of the bacterial ribosome from Escherichia coli in complex with spectinomycin. Deposited 2007-07-21	Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 52-meric	Chain DL 1–144（144 aa）	Not recorded	MG MAGNESIUM ION × 170 SCM SPECTINOMYCIN × 1 ZN ZINC ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions batch；pH 7.5；277 K；MPD, PEG 8000, MgCl2, NH4Cl, spermine, spermidine, TRIS, EDTA, pH 7.5, batch, temperature 277K	Resolution 3.93 Å R-free 0.311
4V57 Crystal structure of the bacterial ribosome from Escherichia coli in complex with spectinomycin and neomycin. Deposited 2007-07-21	Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 52-meric	Chain BL 1–144（144 aa）	Not recorded	NMY NEOMYCIN × 2 MG MAGNESIUM ION × 170 SCM SPECTINOMYCIN × 1 ZN ZINC ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions batch；pH 7.5；277 K；MPD, PEG 8000, MgCl2, NH4Cl, spermine, spermidine, TRIS, EDTA, pH 7.5, batch, temperature 277K	Resolution 3.50 Å R-free 0.306
4V57 Crystal structure of the bacterial ribosome from Escherichia coli in complex with spectinomycin and neomycin. Deposited 2007-07-21	Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 52-meric	Chain DL 1–144（144 aa）	Not recorded	NMY NEOMYCIN × 2 MG MAGNESIUM ION × 170 SCM SPECTINOMYCIN × 1 ZN ZINC ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions batch；pH 7.5；277 K；MPD, PEG 8000, MgCl2, NH4Cl, spermine, spermidine, TRIS, EDTA, pH 7.5, batch, temperature 277K	Resolution 3.50 Å R-free 0.306
4V5B Structure of PDF binding helix in complex with the ribosome. Deposited 2007-11-22	Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Insufficient information Heteromer；Protein × 50 PDB declaration: 53-meric	Chain AL 1–144（144 aa）	Not recorded	MG MAGNESIUM ION × 170	X-RAY DIFFRACTION X-ray crystallization conditions pH 7.5；pH 7.5	Resolution 3.74 Å R-free 0.323
4V5B Structure of PDF binding helix in complex with the ribosome. Deposited 2007-11-22	Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 2 Insufficient information Heteromer；Protein × 49 PDB declaration: 52-meric	Chain CL 1–144（144 aa）	Not recorded	MG MAGNESIUM ION × 173	X-RAY DIFFRACTION X-ray crystallization conditions pH 7.5；pH 7.5	Resolution 3.74 Å R-free 0.323
4V5H E.Coli 70s Ribosome Stalled During Translation Of Tnac Leader Peptide. Deposited 2009-10-26	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 56-meric	Chain BL 2–144（143 aa） Fragment：RESIDUES 2-144	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer 50 MM TRIS-HCL PH 8.0, 10MM MG-ACETATE, 50MM KCL, 10MM NH4CL, 2MM EGTA, 2MM L-TRYPTOPHAN, 10 MM DTT；pH 8；50 MM TRIS-HCL PH 8.0, 10MM MG-ACETATE, 50MM KCL, 10MM NH4CL, 2MM EGTA, 2MM L-TRYPTOPHAN, 10 MM DTT cryo-EM vitrification conditions Cryogen ETHANE；CRYOGEN- ETHANE,	Resolution 5.80 Å
4V5Y Crystal structure of the bacterial ribosome from Escherichia coli in complex with paromomycin and ribosome recycling factor (RRF). Deposited 2007-06-19	Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 53-meric	Chain BL 1–144（144 aa）	Not recorded	MG MAGNESIUM ION × 170 PAR PAROMOMYCIN × 2 ZN ZINC ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions batch；pH 7.5；277 K；MPD, PEG 8000, MgCl2, NH4Cl, spermine, spermidine, TRIS, EDTA, pH 7.5, batch, temperature 277K	Resolution 4.45 Å R-free 0.309
4V5Y Crystal structure of the bacterial ribosome from Escherichia coli in complex with paromomycin and ribosome recycling factor (RRF). Deposited 2007-06-19	Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 53-meric	Chain DL 1–144（144 aa）	Not recorded	MG MAGNESIUM ION × 173 PAR PAROMOMYCIN × 2 ZN ZINC ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions batch；pH 7.5；277 K；MPD, PEG 8000, MgCl2, NH4Cl, spermine, spermidine, TRIS, EDTA, pH 7.5, batch, temperature 277K	Resolution 4.45 Å R-free 0.309
4V64 Crystal structure of the bacterial ribosome from Escherichia coli in complex with hygromycin B. Deposited 2008-06-11	Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 52-meric	Chain BL 1–144（144 aa）	Not recorded	MG MAGNESIUM ION × 170 HYG HYGROMYCIN B × 1 ZN ZINC ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions batch；pH 7.5；277 K；MPD, PEG 8000, MgCl2, NH4Cl, spermine, spermidine, TRIS, EDTA, pH 7.5, batch, temperature 277K	Resolution 3.50 Å R-free 0.318
4V64 Crystal structure of the bacterial ribosome from Escherichia coli in complex with hygromycin B. Deposited 2008-06-11	Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 52-meric	Chain DL 1–144（144 aa）	Not recorded	MG MAGNESIUM ION × 173 HYG HYGROMYCIN B × 1 ZN ZINC ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions batch；pH 7.5；277 K；MPD, PEG 8000, MgCl2, NH4Cl, spermine, spermidine, TRIS, EDTA, pH 7.5, batch, temperature 277K	Resolution 3.50 Å R-free 0.318
4V65 Structure of the E. coli ribosome in the Pre-accommodation state Deposited 2008-08-03	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 57-meric	Chain BE 1–144（144 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 9.00 Å
4V66 Structure of the E. coli ribosome and the tRNAs in Post-accommodation state Deposited 2008-08-03	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 57-meric	Chain BE 1–144（144 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 9.00 Å
4V69 Ternary complex-bound E.coli 70S ribosome. Deposited 2008-12-11	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 58-meric	Chain BL 2–144（143 aa）	Not recorded	GDP GUANOSINE-5'-DIPHOSPHATE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE；Vitrification using FEI Vitrobot; blot 3 seconds before plunging with an offset of -1mm	Resolution 6.70 Å
4V6C Crystal structure of the E. coli 70S ribosome in an intermediate state of ratcheting Deposited 2009-06-27	Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 52-meric	Chain BL 1–144（144 aa）	Not recorded	MG MAGNESIUM ION × 184 ZN ZINC ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions MICROBATCH；291 K；4% PEG 8000, 4% MPD, 3.8 mM MgCl2, 380 mM NH4Cl, 5.5 mM putrescine, 5 mM spermidine, microbatch, temperature 291K	Resolution 3.19 Å R-free 0.252
4V6C Crystal structure of the E. coli 70S ribosome in an intermediate state of ratcheting Deposited 2009-06-27	Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 52-meric	Chain DL 1–144（144 aa）	Not recorded	MG MAGNESIUM ION × 179 ZN ZINC ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions MICROBATCH；291 K；4% PEG 8000, 4% MPD, 3.8 mM MgCl2, 380 mM NH4Cl, 5.5 mM putrescine, 5 mM spermidine, microbatch, temperature 291K	Resolution 3.19 Å R-free 0.252
4V6D Crystal structure of the E. coli 70S ribosome in an intermediate state of ratcheting Deposited 2009-06-27	Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 54-meric	Chain BL 1–144（144 aa）	Not recorded	MG MAGNESIUM ION × 184 ZN ZINC ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions MICROBATCH；pH 7；291 K；4% PEG 8000, 4% MPD, 3.8 mM MgCl2, 380 mM NH4Cl, 5.5 mM putrescine, 5 mM spermidine, pH 7.0, microbatch, temperature 291K	Resolution 3.81 Å R-free 0.253
4V6D Crystal structure of the E. coli 70S ribosome in an intermediate state of ratcheting Deposited 2009-06-27	Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 54-meric	Chain DL 1–144（144 aa）	Not recorded	MG MAGNESIUM ION × 179 ZN ZINC ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions MICROBATCH；pH 7；291 K；4% PEG 8000, 4% MPD, 3.8 mM MgCl2, 380 mM NH4Cl, 5.5 mM putrescine, 5 mM spermidine, pH 7.0, microbatch, temperature 291K	Resolution 3.81 Å R-free 0.253
4V6E Crystal structure of the E. coli 70S ribosome in an intermediate state of ratcheting Deposited 2009-06-28	Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric	Chain BL 1–144（144 aa）	Not recorded	MG MAGNESIUM ION × 184 ZN ZINC ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions MICROBATCH；pH 7；291 K；4% PEG 8000, 4% MPD, 3.8 mM MgCl2, 380 mM NH4Cl, 5.5 mM putrescine, 5 mM spermidine, pH 7.0, microbatch, temperature 291K	Resolution 3.71 Å R-free 0.268
4V6E Crystal structure of the E. coli 70S ribosome in an intermediate state of ratcheting Deposited 2009-06-28	Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric	Chain DL 1–144（144 aa）	Not recorded	MG MAGNESIUM ION × 179 ZN ZINC ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions MICROBATCH；pH 7；291 K；4% PEG 8000, 4% MPD, 3.8 mM MgCl2, 380 mM NH4Cl, 5.5 mM putrescine, 5 mM spermidine, pH 7.0, microbatch, temperature 291K	Resolution 3.71 Å R-free 0.268
4V6K Structural insights into cognate vs. near-cognate discrimination during decoding. Deposited 2011-01-07	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 58-meric	Chain AM 1–144（144 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen NITROGEN	Resolution 8.25 Å
4V6L Structural insights into cognate vs. near-cognate discrimination during decoding. Deposited 2011-01-07	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 58-meric	Chain BM 1–144（144 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen NITROGEN	Resolution 13.20 Å
4V6M Structure of the ribosome-SecYE complex in the membrane environment Deposited 2011-02-08	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 55 PDB declaration: 60-meric	Chain BL 1–144（144 aa）	Not recorded	PEV (1S)-2-{[(2-AMINOETHOXY)(HYDROXY)PHOSPHORYL]OXY}-1-[(PALMITOYLOXY)METHYL]ETHYL STEARATE × 101 PGV (1R)-2-{[{[(2S)-2,3-DIHYDROXYPROPYL]OXY}(HYDROXY)PHOSPHORYL]OXY}-1-[(PALMITOYLOXY)METHYL]ETHYL (11E)-OCTADEC-11-ENOATE × 32	ELECTRON MICROSCOPY cryo-EM buffer 20 mM Hepes (pH 7.2), 100 mM KOAc, 10 mM Mg(OAc)2, 1 mM DTT, 250 microg/ml chloramphenicol；pH 7.2；20 mM Hepes (pH 7.2), 100 mM KOAc, 10 mM Mg(OAc)2, 1 mM DTT, 250 microg/ml chloramphenicol cryo-EM vitrification conditions Cryogen ETHANE；liquid ethane was used as a cryogen	Resolution 7.10 Å
4V6N Structural characterization of mRNA-tRNA translocation intermediates (50S ribosome of class2 of the six classes) Deposited 2011-12-07	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 58-meric	Chain AN 1–144（144 aa）	Not recorded	FME N-FORMYLMETHIONINE × 1 TRP TRYPTOPHAN × 1	ELECTRON MICROSCOPY cryo-EM buffer HiFi buffer (50 mM Tris-HCl, pH 7.5, 70mM NH4Cl, 30 mM KCl, 3.5 mM MgCl2, 0.5 mM spermidine, 8mM putrescine, 2 mM DTT, 3.5 mM MgCl2)；pH 7.5；HiFi buffer (50 mM Tris-HCl, pH 7.5, 70mM NH4Cl, 30 mM KCl, 3.5 mM MgCl2, 0.5 mM spermidine, 8mM putrescine, 2 mM DTT, 3.5 mM MgCl2) cryo-EM vitrification conditions blot for 3 seconds；80 K；Cryogen NITROGEN；Blot for 3 seconds, plunge into liquid nitrogen (Vitrobot)	Resolution 12.10 Å
4V6O Structural characterization of mRNA-tRNA translocation intermediates (class 4a of the six classes) Deposited 2011-12-07	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 58-meric	Chain BN 1–144（144 aa）	Not recorded	TRP TRYPTOPHAN × 1 FME N-FORMYLMETHIONINE × 1	ELECTRON MICROSCOPY cryo-EM buffer HiFi buffer (50 mM Tris-HCl, pH 7.5, 70mM NH4Cl, 30 mM KCl, 3.5 mM MgCl2, 0.5 mM spermidine, 8mM putrescine, 2 mM DTT, 3.5 mM MgCl2)；pH 7.5；HiFi buffer (50 mM Tris-HCl, pH 7.5, 70mM NH4Cl, 30 mM KCl, 3.5 mM MgCl2, 0.5 mM spermidine, 8mM putrescine, 2 mM DTT, 3.5 mM MgCl2) cryo-EM vitrification conditions blot for 3 seconds；80 K；Cryogen NITROGEN；Blot for 3 seconds, plunge into liquid nitrogen (Vitrobot)	Resolution 14.70 Å
4V6P Structural characterization of mRNA-tRNA translocation intermediates (class 4b of the six classes) Deposited 2011-12-08	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 58-meric	Chain BN 1–144（144 aa）	Not recorded	TRP TRYPTOPHAN × 1 FME N-FORMYLMETHIONINE × 1	ELECTRON MICROSCOPY cryo-EM buffer HiFi buffer (50 mM Tris-HCl, pH 7.5, 70mM NH4Cl, 30 mM KCl, 3.5 mM MgCl2, 0.5 mM spermidine, 8mM putrescine, 2 mM DTT, 3.5 mM MgCl2)；pH 7.5；HiFi buffer (50 mM Tris-HCl, pH 7.5, 70mM NH4Cl, 30 mM KCl, 3.5 mM MgCl2, 0.5 mM spermidine, 8mM putrescine, 2 mM DTT, 3.5 mM MgCl2) cryo-EM vitrification conditions blot for 6 seconds；80 K；Cryogen NITROGEN；Blot for 6 seconds, plunge into liquid nitrogen (Vitrobot)	Resolution 13.50 Å
4V6Q Structural characterization of mRNA-tRNA translocation intermediates (class 5 of the six classes) Deposited 2011-12-08	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 58-meric	Chain BN 1–144（144 aa）	Not recorded	TRP TRYPTOPHAN × 1 FME N-FORMYLMETHIONINE × 1	ELECTRON MICROSCOPY cryo-EM buffer HiFi buffer (50 mM Tris-HCl, pH 7.5, 70mM NH4Cl, 30 mM KCl, 3.5 mM MgCl2, 0.5 mM spermidine, 8mM putrescine, 2 mM DTT, 3.5 mM MgCl2)；pH 7.5；HiFi buffer (50 mM Tris-HCl, pH 7.5, 70mM NH4Cl, 30 mM KCl, 3.5 mM MgCl2, 0.5 mM spermidine, 8mM putrescine, 2 mM DTT, 3.5 mM MgCl2) cryo-EM vitrification conditions blot for 3 seconds；80 K；Cryogen NITROGEN；Blot for 3 seconds, plunge into liquid nitrogen (Vitrobot)	Resolution 11.50 Å
4V6R Structural characterization of mRNA-tRNA translocation intermediates (class 6 of the six classes) Deposited 2011-12-08	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 58-meric	Chain BN 1–144（144 aa）	Not recorded	TRP TRYPTOPHAN × 1 FME N-FORMYLMETHIONINE × 1	ELECTRON MICROSCOPY cryo-EM buffer HiFi buffer (50 mM Tris-HCl, pH 7.5, 70mM NH4Cl, 30 mM KCl, 3.5 mM MgCl2, 0.5 mM spermidine, 8mM putrescine, 2 mM DTT, 3.5 mM MgCl2)；pH 7.5；HiFi buffer (50 mM Tris-HCl, pH 7.5, 70mM NH4Cl, 30 mM KCl, 3.5 mM MgCl2, 0.5 mM spermidine, 8mM putrescine, 2 mM DTT, 3.5 mM MgCl2) cryo-EM vitrification conditions blot for 3 seconds；80 K；Cryogen NITROGEN；Blot for 3 seconds, plunge into liquid nitrogen (Vitrobot)	Resolution 11.50 Å
4V6S Structural characterization of mRNA-tRNA translocation intermediates (class 3 of the six classes) Deposited 2011-12-09	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 57-meric	Chain AN 1–144（144 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer HiFi buffer (50 mM Tris-HCl, pH 7.5, 70mM NH4Cl, 30 mM KCl, 3.5 mM MgCl2, 0.5 mM spermidine, 8mM putrescine, 2 mM DTT, 3.5 mM MgCl2)；pH 7.5；HiFi buffer (50 mM Tris-HCl, pH 7.5, 70mM NH4Cl, 30 mM KCl, 3.5 mM MgCl2, 0.5 mM spermidine, 8mM putrescine, 2 mM DTT, 3.5 mM MgCl2) cryo-EM vitrification conditions blot for 3 seconds；80 K；Cryogen NITROGEN；Blot for 3 seconds, plunge into liquid nitrogen (Vitrobot)	Resolution 13.10 Å
4V6T Structure of the bacterial ribosome complexed by tmRNA-SmpB and EF-G during translocation and MLD-loading Deposited 2012-01-27	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 56-meric	Chain BL 2–144（143 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6 cryo-EM vitrification conditions blot for 5-10 seconds before plunging；96.15 K；Cryogen ETHANE；blot for 5-10 seconds before plunging into liquid ethane (FEI Vitrobot)	Resolution 8.30 Å
4V6V Tetracycline resistance protein Tet(O) bound to the ribosome Deposited 2013-02-25	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 53 PDB declaration: 58-meric	Chain BP 1–144（144 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM vitrification conditions Cryogen ETHANE	Resolution 9.80 Å
4V6Y E. coli 70S-fMetVal-tRNAVal-tRNAfMet complex in classic pre-translocation state (pre1a) Deposited 2013-10-14	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric	Chain BL 1–144（144 aa）	Not recorded	VAL VALINE × 1 FME N-FORMYLMETHIONINE × 1	ELECTRON MICROSCOPY cryo-EM buffer 50 mM Tris-HCl, 70 mM NH4Cl, 30 mM KCl, 7 mM MgCl2, 0.6 mM spermine, 0.4 mM spermidine；pH 7.5；50 mM Tris-HCl, 70 mM NH4Cl, 30 mM KCl, 7 mM MgCl2, 0.6 mM spermine, 0.4 mM spermidine cryo-EM vitrification conditions Manual blotting for about 2 seconds；Cryogen ETHANE；Manual blotting for about 2 seconds prior to plunging into liquid ethane (custom-built CEVS vitrification instrument with dew point temperature adjusted to 18 degrees C)	Resolution 12.00 Å
4V6Z E. coli 70S-fMetVal-tRNAVal-tRNAfMet complex in classic pre-translocation state (pre1b) Deposited 2013-10-14	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric	Chain BL 1–144（144 aa）	Not recorded	VAL VALINE × 1 FME N-FORMYLMETHIONINE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 12.00 Å
4V70 E. coli 70S-fMetVal-tRNAVal-tRNAfMet complex in intermediate pre-translocation state (pre3) Deposited 2013-10-14	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric	Chain BL 1–144（144 aa）	Not recorded	VAL VALINE × 1 FME N-FORMYLMETHIONINE × 1	ELECTRON MICROSCOPY cryo-EM buffer 50 mM Tris-HCl, 70 mM NH4Cl, 30 mM KCl, 7 mM MgCl2, 0.6 mM spermine, 0.4 mM spermidine；pH 7.5；50 mM Tris-HCl, 70 mM NH4Cl, 30 mM KCl, 7 mM MgCl2, 0.6 mM spermine, 0.4 mM spermidine cryo-EM vitrification conditions Manual blotting for about 2 seconds；Cryogen ETHANE；Manual blotting for about 2 seconds prior to plunging into liquid ethane (custom-built CEVS vitrification instrument with dew point temperature adjusted to 18 degrees C)	Resolution 17.00 Å
4V71 E. coli 70S-fMetVal-tRNAVal-tRNAfMet complex in intermediate pre-translocation state (pre2) Deposited 2013-10-14	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric	Chain BL 1–144（144 aa）	Not recorded	VAL VALINE × 1 FME N-FORMYLMETHIONINE × 1	ELECTRON MICROSCOPY cryo-EM buffer 50 mM Tris-HCl, 70 mM NH4Cl, 30 mM KCl, 7 mM MgCl2, 0.6 mM spermine, 0.4 mM spermidine；pH 7.5；50 mM Tris-HCl, 70 mM NH4Cl, 30 mM KCl, 7 mM MgCl2, 0.6 mM spermine, 0.4 mM spermidine cryo-EM vitrification conditions Manual blotting for about 2 seconds；Cryogen ETHANE；Manual blotting for about 2 seconds prior to plunging into liquid ethane (custom-built CEVS vitrification instrument with dew point temperature adjusted to 18 degrees C)	Resolution 20.00 Å
4V72 E. coli 70S-fMetVal-tRNAVal-tRNAfMet complex in hybrid pre-translocation state (pre4) Deposited 2013-10-14	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric	Chain BL 1–144（144 aa）	Not recorded	VAL VALINE × 1 FME N-FORMYLMETHIONINE × 1	ELECTRON MICROSCOPY cryo-EM buffer 50 mM Tris-HCl, 70 mM NH4Cl, 30 mM KCl, 7 mM MgCl2, 0.6 mM spermine, 0.4 mM spermidine；pH 7.5；50 mM Tris-HCl, 70 mM NH4Cl, 30 mM KCl, 7 mM MgCl2, 0.6 mM spermine, 0.4 mM spermidine cryo-EM vitrification conditions Manual blotting for about 2 seconds；Cryogen ETHANE；Manual blotting for about 2 seconds prior to plunging into liquid ethane (custom-built CEVS vitrification instrument with dew point temperature adjusted to 18 degrees C)	Resolution 13.00 Å
4V73 E. coli 70S-fMetVal-tRNAVal-tRNAfMet complex in hybrid pre-translocation state (pre5a) Deposited 2013-10-14	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric	Chain BL 1–144（144 aa）	Not recorded	VAL VALINE × 1 FME N-FORMYLMETHIONINE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 15.00 Å
4V74 70S-fMetVal-tRNAVal-tRNAfMet complex in hybrid pre-translocation state (pre5b) Deposited 2013-10-14	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric	Chain BL 1–144（144 aa）	Not recorded	VAL VALINE × 1 FME N-FORMYLMETHIONINE × 1	ELECTRON MICROSCOPY cryo-EM buffer 50 mM Tris-HCl, 70 mM NH4Cl, 30 mM KCl, 7 mM MgCl2, 0.6 mM spermine, 0.4 mM spermidine；pH 7.5；50 mM Tris-HCl, 70 mM NH4Cl, 30 mM KCl, 7 mM MgCl2, 0.6 mM spermine, 0.4 mM spermidine cryo-EM vitrification conditions Manual blotting for about 2 seconds；Cryogen ETHANE；Manual blotting for about 2 seconds prior to plunging into liquid ethane (custom-built CEVS vitrification instrument with dew point temperature adjusted to 18 degrees C)	Resolution 17.00 Å
4V75 E. coli 70S-fMetVal-tRNAVal-tRNAfMet complex in classic post-translocation state (post1) Deposited 2013-10-14	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric	Chain BL 1–144（144 aa）	Not recorded	VAL VALINE × 1 FME N-FORMYLMETHIONINE × 1	ELECTRON MICROSCOPY cryo-EM buffer 50 mM Tris-HCl, 70 mM NH4Cl, 30 mM KCl, 7 mM MgCl2, 0.6 mM spermine, 0.4 mM spermidine；pH 7.5；50 mM Tris-HCl, 70 mM NH4Cl, 30 mM KCl, 7 mM MgCl2, 0.6 mM spermine, 0.4 mM spermidine cryo-EM vitrification conditions Manual blotting for about 2 seconds；Cryogen ETHANE；Manual blotting for about 2 seconds prior to plunging into liquid ethane (custom-built CEVS vitrification instrument with dew point temperature adjusted to 18 degrees C)	Resolution 12.00 Å
4V76 E. coli 70S-fMetVal-tRNAVal-tRNAfMet complex in intermediate post-translocation state (post2a) Deposited 2013-10-14	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric	Chain BL 1–144（144 aa）	Not recorded	VAL VALINE × 1 FME N-FORMYLMETHIONINE × 1	ELECTRON MICROSCOPY cryo-EM buffer 50 mM Tris-HCl, 70 mM NH4Cl, 30 mM KCl, 7 mM MgCl2, 0.6 mM spermine, 0.4 mM spermidine；pH 7.5；50 mM Tris-HCl, 70 mM NH4Cl, 30 mM KCl, 7 mM MgCl2, 0.6 mM spermine, 0.4 mM spermidine cryo-EM vitrification conditions Manual blotting for about 2 seconds；Cryogen ETHANE；Manual blotting for about 2 seconds prior to plunging into liquid ethane (custom-built CEVS vitrification instrument with dew point temperature adjusted to 18 degrees C)	Resolution 17.00 Å
4V77 E. coli 70S-fMetVal-tRNAVal-tRNAfMet complex in intermediate post-translocation state (post2b) Deposited 2013-10-14	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric	Chain BL 1–144（144 aa）	Not recorded	VAL VALINE × 1 FME N-FORMYLMETHIONINE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 17.00 Å
4V78 E. coli 70S-fMetVal-tRNAVal-tRNAfMet complex in intermediate post-translocation state (post3a) Deposited 2013-10-14	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric	Chain BL 1–144（144 aa）	Not recorded	VAL VALINE × 1 FME N-FORMYLMETHIONINE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 20.00 Å
4V79 E. coli 70S-fMetVal-tRNAVal-tRNAfMet complex in intermediate post-translocation state (post3b) Deposited 2013-10-14	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric	Chain BL 1–144（144 aa）	Not recorded	VAL VALINE × 1 FME N-FORMYLMETHIONINE × 1	ELECTRON MICROSCOPY cryo-EM buffer 50 mM Tris-HCl, 70 mM NH4Cl, 30 mM KCl, 7 mM MgCl2, 0.6 mM spermine, 0.4 mM spermidine；pH 7.5；50 mM Tris-HCl, 70 mM NH4Cl, 30 mM KCl, 7 mM MgCl2, 0.6 mM spermine, 0.4 mM spermidine cryo-EM vitrification conditions Manual blotting for about 2 seconds；Cryogen ETHANE；Manual blotting for about 2 seconds prior to plunging into liquid ethane (custom-built CEVS vitrification instrument with dew point temperature adjusted to 18 degrees C)	Resolution 15.00 Å
4V7A E. coli 70S-fMetVal-tRNAVal post-translocation complex (post4) Deposited 2013-10-14	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric	Chain BL 1–144（144 aa）	Not recorded	VAL VALINE × 1 FME N-FORMYLMETHIONINE × 1	ELECTRON MICROSCOPY cryo-EM buffer 50 mM Tris-HCl, 70 mM NH4Cl, 30 mM KCl, 7 mM MgCl2, 0.6 mM spermine, 0.4 mM spermidine；pH 7.5；50 mM Tris-HCl, 70 mM NH4Cl, 30 mM KCl, 7 mM MgCl2, 0.6 mM spermine, 0.4 mM spermidine cryo-EM vitrification conditions Manual blotting for about 2 seconds；Cryogen ETHANE；Manual blotting for about 2 seconds prior to plunging into liquid ethane (custom-built CEVS vitrification instrument with dew point temperature adjusted to 18 degrees C)	Resolution 9.00 Å
4V7B Visualization of two tRNAs trapped in transit during EF-G-mediated translocation Deposited 2013-10-27	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 58-meric	Chain BL 1–144（144 aa）	Not recorded	FUA FUSIDIC ACID × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1	ELECTRON MICROSCOPY cryo-EM buffer 80 mM HEPES potassium, 75 mM NH4Cl, 10 mM MgCl2, 6 mM BME；pH 7.6；80 mM HEPES potassium, 75 mM NH4Cl, 10 mM MgCl2, 6 mM BME cryo-EM vitrification conditions blot for 5-10 seconds before plunging；96 K；Cryogen ETHANE；Blot for 5-10 seconds before plunging into liquid ethane (FEI VITROBOT MARK I)	Resolution 6.80 Å
4V7C Structure of the Ribosome with Elongation Factor G Trapped in the Pre-Translocation State (pre-translocation 70S*tRNA structure) Deposited 2013-11-20	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 58-meric	Chain BN 1–144（144 aa）	Not recorded	ZN ZINC ION × 1	ELECTRON MICROSCOPY cryo-EM buffer Polymix buffer；pH 7.6；10 mM HEPES-KOH, 5 mM MgCl2, 90 mM NH4Cl, 2 mM spermidine, 0.1 mM spermine, 6 mM BME, 0.5 mM viomycin, 0.5 mM GTP, 0.5 mM fusidic acid cryo-EM vitrification conditions Freshly glow-disharged grids were loaded into an FEI Mark II Vitrobot and equilibrated to 95% relative humidity at 22 degrees Celsius. 2 microliters of sample was applied through the side port, blotted for 7 seconds with a positional offset of 2, and plunged into liquid ethane.；Cryogen ETHANE；Freshly glow-disharged grids were loaded into an FEI Mark II Vitrobot and equilibrated to 95% relative humidity at 22 degrees Celsius. 2 microliters of sample was applied through the side port, blotted for 7 seconds with a positional offset of 2, and plunged into liquid ethane.	Resolution 7.60 Å
4V7D Structure of the Ribosome with Elongation Factor G Trapped in the Pre-Translocation State (pre-translocation 70StRNAEF-G structure) Deposited 2013-11-21	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 54 PDB declaration: 60-meric	Chain AO 1–144（144 aa）	Not recorded	ZN ZINC ION × 1	ELECTRON MICROSCOPY cryo-EM buffer Polymix buffer；pH 7.6；10 mM HEPES-KOH, 5 mM MgCl2, 90 mM NH4Cl, 2 mM spermidine, 0.1 mM spermine, 6 mM BME, 0.5 mM viomycin, 0.5 mM GTP, 0.5 mM fusidic acid cryo-EM vitrification conditions Freshly glow-disharged grids were loaded into an FEI Mark II Vitrobot and equilibrated to 95% relative humidity at 22 degrees Celsius. 2 microliters of sample was applied through the side port, blotted for 7 seconds with a positional offset of 2, and plunged into liquid ethane.；Cryogen ETHANE；Freshly glow-disharged grids were loaded into an FEI Mark II Vitrobot and equilibrated to 95% relative humidity at 22 degrees Celsius. 2 microliters of sample was applied through the side port, blotted for 7 seconds with a positional offset of 2, and plunged into liquid ethane.	Resolution 7.60 Å
4V7I Ribosome-SecY complex. Deposited 2009-10-21	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 53 PDB declaration: 56-meric	Chain AL 1–144（144 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer 50 MM HEPES- KOH PH 7.5, 100 MM KOAC, 10 MM MG(OAC)2, 0.05% DDM；pH 7.5；50 MM HEPES- KOH PH 7.5, 100 MM KOAC, 10 MM MG(OAC)2, 0.05% DDM cryo-EM vitrification conditions Cryogen ETHANE；THE SPECIMENS WERE PLUNGE FROZEN IN LIQUID ETHANE AT 4 DEGREES C AT AN RH OF ~90-95%.	Resolution 9.60 Å
4V7S Crystal structure of the E. coli ribosome bound to telithromycin. Deposited 2010-08-05	Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 52-meric	Chain BL 2–144（143 aa）	Not recorded	MG MAGNESIUM ION × 181 TEL TELITHROMYCIN × 1 ZN ZINC ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions MICROBATCH；pH 6.5；291 K；PEG8k, MPD, pH 6.5, microbatch, temperature 291K	Resolution 3.25 Å R-free 0.245
4V7S Crystal structure of the E. coli ribosome bound to telithromycin. Deposited 2010-08-05	Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 52-meric	Chain DL 2–144（143 aa）	Not recorded	MG MAGNESIUM ION × 179 ZN ZINC ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions MICROBATCH；pH 6.5；291 K；PEG8k, MPD, pH 6.5, microbatch, temperature 291K	Resolution 3.25 Å R-free 0.245
4V7T Crystal structure of the E. coli ribosome bound to chloramphenicol. Deposited 2010-08-14	Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 52-meric	Chain BL 2–144（143 aa）	Not recorded	MG MAGNESIUM ION × 183 CLM CHLORAMPHENICOL × 1 ZN ZINC ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions MICROBATCH；pH 6.5；291 K；PEG8k, MPD, pH 6.5, microbatch, temperature 291K	Resolution 3.19 Å R-free 0.252
4V7T Crystal structure of the E. coli ribosome bound to chloramphenicol. Deposited 2010-08-14	Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 52-meric	Chain DL 2–144（143 aa）	Not recorded	MG MAGNESIUM ION × 179 ZN ZINC ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions MICROBATCH；pH 6.5；291 K；PEG8k, MPD, pH 6.5, microbatch, temperature 291K	Resolution 3.19 Å R-free 0.252
4V7U Crystal structure of the E. coli ribosome bound to erythromycin. Deposited 2010-08-15	Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 52-meric	Chain BL 2–144（143 aa）	Not recorded	MG MAGNESIUM ION × 182 ERY ERYTHROMYCIN A × 1 ZN ZINC ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions MICROBATCH；pH 6.5；291 K；4.3% PEG 8000, 4.9% MPD, 3.8 mM MgCl2, 380 mM NH4Cl, 5.5 mM putrescine, 5 mM spermidine, 10 mM Tris, 20 mM MES pH 6.5. 0.25 mM EDTA, microbatch, temperature 291K	Resolution 3.10 Å R-free 0.254
4V7U Crystal structure of the E. coli ribosome bound to erythromycin. Deposited 2010-08-15	Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 52-meric	Chain DL 2–144（143 aa）	Not recorded	MG MAGNESIUM ION × 179 ZN ZINC ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions MICROBATCH；pH 6.5；291 K；4.3% PEG 8000, 4.9% MPD, 3.8 mM MgCl2, 380 mM NH4Cl, 5.5 mM putrescine, 5 mM spermidine, 10 mM Tris, 20 mM MES pH 6.5. 0.25 mM EDTA, microbatch, temperature 291K	Resolution 3.10 Å R-free 0.254
4V7V Crystal structure of the E. coli ribosome bound to clindamycin. Deposited 2010-08-16	Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 52-meric	Chain BL 2–144（143 aa）	Not recorded	MG MAGNESIUM ION × 182 CLY CLINDAMYCIN × 1 ZN ZINC ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions MICROBATCH；291 K；4.3% PEG 8000, 4.9% MPD, 10 mM Tris, 20 mM MES, pH 6.5-7.0, 3.8 mM MgCl2, 380 mM NH4Cl, 5.5 mM putrescine, 5 mM spermidine, 0.25 mM EDTA, microbatch, temperature 291K	Resolution 3.29 Å R-free 0.241
4V7V Crystal structure of the E. coli ribosome bound to clindamycin. Deposited 2010-08-16	Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 52-meric	Chain DL 2–144（143 aa）	Not recorded	MG MAGNESIUM ION × 179 ZN ZINC ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions MICROBATCH；291 K；4.3% PEG 8000, 4.9% MPD, 10 mM Tris, 20 mM MES, pH 6.5-7.0, 3.8 mM MgCl2, 380 mM NH4Cl, 5.5 mM putrescine, 5 mM spermidine, 0.25 mM EDTA, microbatch, temperature 291K	Resolution 3.29 Å R-free 0.241
4V89 Crystal Structure of Release Factor RF3 Trapped in the GTP State on a Rotated Conformation of the Ribosome (without viomycin) Deposited 2011-11-17	Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 54 PDB declaration: 58-meric	Chain BP 1–144（144 aa）	Not recorded	GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；295 K；Tris Ac PH.7.0 25-35 mM KCL 6.1% PEG 20000 %1 glycerol 50mM sucrose , VAPOR DIFFUSION, SITTING DROP, temperature 295K	Resolution 3.70 Å R-free 0.290
4V9C Allosteric control of the ribosome by small-molecule antibiotics Deposited 2012-07-25	Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 54-meric	Chain BL 1–144（144 aa）	Not recorded	MG MAGNESIUM ION × 224 NMY NEOMYCIN × 10 ZN ZINC ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions pH 6.5；291 K；PEG800, MPD, pH 6.5, microbatch, temperature 291K	Resolution 3.30 Å R-free 0.255
4V9C Allosteric control of the ribosome by small-molecule antibiotics Deposited 2012-07-25	Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric	Chain DL 1–144（144 aa）	Not recorded	MG MAGNESIUM ION × 266 NMY NEOMYCIN × 8 ZN ZINC ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions pH 6.5；291 K；PEG800, MPD, pH 6.5, microbatch, temperature 291K	Resolution 3.30 Å R-free 0.255
4V9D Structures of the bacterial ribosome in classical and hybrid states of tRNA binding Deposited 2012-07-31	Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric	Chain CL 2–144（143 aa）	Not recorded	MG MAGNESIUM ION × 271 ZN ZINC ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions MICROBATCH；pH 6.5；291 K；PEG8000, MPD, pH 6.5, microbatch, temperature 291K X-ray crystallization conditions MICROBATCH；pH 6.5；291 K；PEG8000, MPD, pH 6.5, microbatch, temperature 291K X-ray crystallization conditions MICROBATCH；pH 6.5；291 K；PEG8000, MPD, pH 6.5, microbatch, temperature 291K X-ray crystallization conditions MICROBATCH；pH 6.5；291 K；PEG8000, MPD, pH 6.5, microbatch, temperature 291K X-ray crystallization conditions MICROBATCH；pH 6.5；291 K；PEG8000, MPD, pH 6.5, microbatch, temperature 291K	Resolution 3.00 Å R-free 0.260
4V9D Structures of the bacterial ribosome in classical and hybrid states of tRNA binding Deposited 2012-07-31	Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 54-meric	Chain DL 2–144（143 aa）	Not recorded	MG MAGNESIUM ION × 227 ZN ZINC ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions MICROBATCH；pH 6.5；291 K；PEG8000, MPD, pH 6.5, microbatch, temperature 291K X-ray crystallization conditions MICROBATCH；pH 6.5；291 K；PEG8000, MPD, pH 6.5, microbatch, temperature 291K X-ray crystallization conditions MICROBATCH；pH 6.5；291 K；PEG8000, MPD, pH 6.5, microbatch, temperature 291K X-ray crystallization conditions MICROBATCH；pH 6.5；291 K；PEG8000, MPD, pH 6.5, microbatch, temperature 291K X-ray crystallization conditions MICROBATCH；pH 6.5；291 K；PEG8000, MPD, pH 6.5, microbatch, temperature 291K	Resolution 3.00 Å R-free 0.260
4V9O Control of ribosomal subunit rotation by elongation factor G Deposited 2013-05-03	Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 53 PDB declaration: 56-meric	Chain AL 1–144（144 aa）	Not recorded	MG MAGNESIUM ION × 187 ZN ZINC ION × 1 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1	X-RAY DIFFRACTION X-ray crystallization conditions MICROBATCH；pH 6.5；291 K；PEG8k, MPD, KSCN, pH 6.5, microbatch, temperature 291K X-ray crystallization conditions MICROBATCH；pH 6.5；291 K；PEG8k, MPD, KSCN, pH 6.5, microbatch, temperature 291K X-ray crystallization conditions MICROBATCH；pH 6.5；291 K；PEG8k, MPD, KSCN, pH 6.5, microbatch, temperature 291K X-ray crystallization conditions MICROBATCH；pH 6.5；291 K；PEG8k, KSCN, pH 6.5, microbatch, temperature 291K X-ray crystallization conditions MICROBATCH；pH 6.5；291 K；PEG8k, KSCN, pH 6.5, microbatch, temperature 291K	Resolution 2.90 Å R-free 0.272
4V9O Control of ribosomal subunit rotation by elongation factor G Deposited 2013-05-03	Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 2 Protein–RNA Heteromer；Protein × 52 PDB declaration: 55-meric	Chain CL 1–144（144 aa）	Not recorded	MG MAGNESIUM ION × 187 ZN ZINC ION × 1 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1	X-RAY DIFFRACTION X-ray crystallization conditions MICROBATCH；pH 6.5；291 K；PEG8k, MPD, KSCN, pH 6.5, microbatch, temperature 291K X-ray crystallization conditions MICROBATCH；pH 6.5；291 K；PEG8k, MPD, KSCN, pH 6.5, microbatch, temperature 291K X-ray crystallization conditions MICROBATCH；pH 6.5；291 K；PEG8k, MPD, KSCN, pH 6.5, microbatch, temperature 291K X-ray crystallization conditions MICROBATCH；pH 6.5；291 K；PEG8k, KSCN, pH 6.5, microbatch, temperature 291K X-ray crystallization conditions MICROBATCH；pH 6.5；291 K；PEG8k, KSCN, pH 6.5, microbatch, temperature 291K	Resolution 2.90 Å R-free 0.272
4V9O Control of ribosomal subunit rotation by elongation factor G Deposited 2013-05-03	Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 3 Protein–RNA Heteromer；Protein × 52 PDB declaration: 55-meric	Chain EL 1–144（144 aa）	Not recorded	MG MAGNESIUM ION × 187 ZN ZINC ION × 1 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1	X-RAY DIFFRACTION X-ray crystallization conditions MICROBATCH；pH 6.5；291 K；PEG8k, MPD, KSCN, pH 6.5, microbatch, temperature 291K X-ray crystallization conditions MICROBATCH；pH 6.5；291 K；PEG8k, MPD, KSCN, pH 6.5, microbatch, temperature 291K X-ray crystallization conditions MICROBATCH；pH 6.5；291 K；PEG8k, MPD, KSCN, pH 6.5, microbatch, temperature 291K X-ray crystallization conditions MICROBATCH；pH 6.5；291 K；PEG8k, KSCN, pH 6.5, microbatch, temperature 291K X-ray crystallization conditions MICROBATCH；pH 6.5；291 K；PEG8k, KSCN, pH 6.5, microbatch, temperature 291K	Resolution 2.90 Å R-free 0.272
4V9O Control of ribosomal subunit rotation by elongation factor G Deposited 2013-05-03	Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 4 Protein–RNA Heteromer；Protein × 51 PDB declaration: 54-meric	Chain GL 1–144（144 aa）	Not recorded	MG MAGNESIUM ION × 187 ZN ZINC ION × 1 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1	X-RAY DIFFRACTION X-ray crystallization conditions MICROBATCH；pH 6.5；291 K；PEG8k, MPD, KSCN, pH 6.5, microbatch, temperature 291K X-ray crystallization conditions MICROBATCH；pH 6.5；291 K；PEG8k, MPD, KSCN, pH 6.5, microbatch, temperature 291K X-ray crystallization conditions MICROBATCH；pH 6.5；291 K；PEG8k, MPD, KSCN, pH 6.5, microbatch, temperature 291K X-ray crystallization conditions MICROBATCH；pH 6.5；291 K；PEG8k, KSCN, pH 6.5, microbatch, temperature 291K X-ray crystallization conditions MICROBATCH；pH 6.5；291 K；PEG8k, KSCN, pH 6.5, microbatch, temperature 291K	Resolution 2.90 Å R-free 0.272
4V9P Control of ribosomal subunit rotation by elongation factor G Deposited 2013-05-03	Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 55-meric	Chain AL 1–144（144 aa）	Not recorded	MG MAGNESIUM ION × 185 ZN ZINC ION × 1 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1	X-RAY DIFFRACTION X-ray crystallization conditions MICROBATCH；pH 6.5；291 K；PEG8k, MPD, KSCN, pH 6.5, microbatch, temperature 291K X-ray crystallization conditions MICROBATCH；pH 6.5；291 K；PEG8k, MPD, KSCN, pH 6.5, microbatch, temperature 291K X-ray crystallization conditions MICROBATCH；pH 6.5；291 K；PEG8k, MPD, KSCN, pH 6.5, microbatch, temperature 291K X-ray crystallization conditions MICROBATCH；pH 6.5；291 K；PEG8k, KSCN, pH 6.5, microbatch, temperature 291K X-ray crystallization conditions MICROBATCH；pH 6.5；291 K；PEG8k, KSCN, pH 6.5, microbatch, temperature 291K	Resolution 2.90 Å R-free 0.267
4V9P Control of ribosomal subunit rotation by elongation factor G Deposited 2013-05-03	Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 2 Protein–RNA Heteromer；Protein × 51 PDB declaration: 54-meric	Chain CL 1–144（144 aa）	Not recorded	MG MAGNESIUM ION × 185 ZN ZINC ION × 1 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1	X-RAY DIFFRACTION X-ray crystallization conditions MICROBATCH；pH 6.5；291 K；PEG8k, MPD, KSCN, pH 6.5, microbatch, temperature 291K X-ray crystallization conditions MICROBATCH；pH 6.5；291 K；PEG8k, MPD, KSCN, pH 6.5, microbatch, temperature 291K X-ray crystallization conditions MICROBATCH；pH 6.5；291 K；PEG8k, MPD, KSCN, pH 6.5, microbatch, temperature 291K X-ray crystallization conditions MICROBATCH；pH 6.5；291 K；PEG8k, KSCN, pH 6.5, microbatch, temperature 291K X-ray crystallization conditions MICROBATCH；pH 6.5；291 K；PEG8k, KSCN, pH 6.5, microbatch, temperature 291K	Resolution 2.90 Å R-free 0.267
4V9P Control of ribosomal subunit rotation by elongation factor G Deposited 2013-05-03	Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 3 Protein–RNA Heteromer；Protein × 52 PDB declaration: 55-meric	Chain EL 1–144（144 aa）	Not recorded	MG MAGNESIUM ION × 185 ZN ZINC ION × 1 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1	X-RAY DIFFRACTION X-ray crystallization conditions MICROBATCH；pH 6.5；291 K；PEG8k, MPD, KSCN, pH 6.5, microbatch, temperature 291K X-ray crystallization conditions MICROBATCH；pH 6.5；291 K；PEG8k, MPD, KSCN, pH 6.5, microbatch, temperature 291K X-ray crystallization conditions MICROBATCH；pH 6.5；291 K；PEG8k, MPD, KSCN, pH 6.5, microbatch, temperature 291K X-ray crystallization conditions MICROBATCH；pH 6.5；291 K；PEG8k, KSCN, pH 6.5, microbatch, temperature 291K X-ray crystallization conditions MICROBATCH；pH 6.5；291 K；PEG8k, KSCN, pH 6.5, microbatch, temperature 291K	Resolution 2.90 Å R-free 0.267
4V9P Control of ribosomal subunit rotation by elongation factor G Deposited 2013-05-03	Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 4 Protein–RNA Heteromer；Protein × 50 PDB declaration: 53-meric	Chain GL 1–144（144 aa）	Not recorded	MG MAGNESIUM ION × 185 ZN ZINC ION × 1 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1	X-RAY DIFFRACTION X-ray crystallization conditions MICROBATCH；pH 6.5；291 K；PEG8k, MPD, KSCN, pH 6.5, microbatch, temperature 291K X-ray crystallization conditions MICROBATCH；pH 6.5；291 K；PEG8k, MPD, KSCN, pH 6.5, microbatch, temperature 291K X-ray crystallization conditions MICROBATCH；pH 6.5；291 K；PEG8k, MPD, KSCN, pH 6.5, microbatch, temperature 291K X-ray crystallization conditions MICROBATCH；pH 6.5；291 K；PEG8k, KSCN, pH 6.5, microbatch, temperature 291K X-ray crystallization conditions MICROBATCH；pH 6.5；291 K；PEG8k, KSCN, pH 6.5, microbatch, temperature 291K	Resolution 2.90 Å R-free 0.267
4WF1 Crystal structure of the E. coli ribosome bound to negamycin. Deposited 2014-09-11	Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 53-meric	Chain BL 2–144（143 aa）	Not recorded	MG MAGNESIUM ION × 271 ZN ZINC ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions MICROBATCH；pH 6.5；291 K；PEG8k, MPD	Resolution 3.09 Å R-free 0.244
4WF1 Crystal structure of the E. coli ribosome bound to negamycin. Deposited 2014-09-11	Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 52-meric	Chain DL 2–144（143 aa）	Not recorded	MG MAGNESIUM ION × 228 ZN ZINC ION × 1 NEG NEGAMYCIN × 1	X-RAY DIFFRACTION X-ray crystallization conditions MICROBATCH；pH 6.5；291 K；PEG8k, MPD	Resolution 3.09 Å R-free 0.244
4WOI 4,5-linked aminoglycoside antibiotics regulate the bacterial ribosome by targeting dynamic conformational processes within intersubunit bridge B2 Deposited 2014-10-15	Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 56-meric	Chain BL 1–144（144 aa）	Not recorded	MG MAGNESIUM ION × 269 PAR PAROMOMYCIN × 6 ZN ZINC ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions MICROBATCH；pH 6.5；291 K；PEG8k, MPD, KCl, KSCN	Resolution 3.00 Å R-free 0.253
4WOI 4,5-linked aminoglycoside antibiotics regulate the bacterial ribosome by targeting dynamic conformational processes within intersubunit bridge B2 Deposited 2014-10-15	Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 54-meric	Chain CL 1–144（144 aa）	Not recorded	MG MAGNESIUM ION × 226 PAR PAROMOMYCIN × 7 ZN ZINC ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions MICROBATCH；pH 6.5；291 K；PEG8k, MPD, KCl, KSCN	Resolution 3.00 Å R-free 0.253
4WWW Crystal structure of the E. coli ribosome bound to CEM-101 Deposited 2014-11-12	Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 52-meric	Chain RL 2–144（143 aa）	Not recorded	MG MAGNESIUM ION × 182 EM1 (3aS,4R,7S,9R,10R,11R,13R,15R,15aR)-1-{4-[4-(3-aminophenyl)-1H-1,2,3-triazol-1-yl]butyl}-4-ethyl-7-fluoro-11-methoxy-3a ,7,9,11,13,15-hexamethyl-2,6,8,14-tetraoxotetradecahydro-2H-oxacyclotetradecino[4,3-d][1,3]oxazol-10-yl 3,4,6-trideoxy-3-(dimethylamino)-beta-D-xylo-hexopyranoside × 1 ZN ZINC ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions MICROBATCH；pH 6.5；291 K；PEG8k, MPD	Resolution 3.10 Å R-free 0.252
4WWW Crystal structure of the E. coli ribosome bound to CEM-101 Deposited 2014-11-12	Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 52-meric	Chain YL 2–144（143 aa）	Not recorded	MG MAGNESIUM ION × 179 ZN ZINC ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions MICROBATCH；pH 6.5；291 K；PEG8k, MPD	Resolution 3.10 Å R-free 0.252
4YBB High-resolution structure of the Escherichia coli ribosome Deposited 2015-02-18	Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 53-meric	Chain DM 1–144（144 aa）	Not recorded	MG MAGNESIUM ION × 263 PG4 TETRAETHYLENE GLYCOL × 6 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 14 PUT 1,4-DIAMINOBUTANE × 16 ZN ZINC ION × 2 PEG DI(HYDROXYETHYL)ETHER × 11 SPD SPERMIDINE × 4 1PE PENTAETHYLENE GLYCOL × 2 PGE TRIETHYLENE GLYCOL × 9 ACY ACETIC ACID × 3 EDO 1,2-ETHANEDIOL × 12 GUN GUANINE × 1 TRS 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL × 1	X-RAY DIFFRACTION X-ray crystallization conditions MICROBATCH；pH 6.5；291 K；PEG8k, MPD	Resolution 2.10 Å R-free 0.234
4YBB High-resolution structure of the Escherichia coli ribosome Deposited 2015-02-18	Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 52-meric	Chain CM 1–144（144 aa）	Not recorded	MG MAGNESIUM ION × 200 PG4 TETRAETHYLENE GLYCOL × 1 ZN ZINC ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions MICROBATCH；pH 6.5；291 K；PEG8k, MPD	Resolution 2.10 Å R-free 0.234
5ADY Cryo-EM structures of the 50S ribosome subunit bound with HflX Deposited 2015-08-25	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 32 PDB declaration: 34-meric	Chain L 1–144（144 aa）	Not recorded	GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1	ELECTRON MICROSCOPY cryo-EM buffer 20MM TRIS-HCL, 100MM NH4CL, 10MM MGCL2；pH 7.5；20MM TRIS-HCL, 100MM NH4CL, 10MM MGCL2 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 4.50 Å
5AFI 2.9A Structure of E. coli ribosome-EF-TU complex by cs-corrected cryo-EM Deposited 2015-01-22	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 59-meric	Chain L 1–144（144 aa）	Not recorded	MG MAGNESIUM ION × 333 CL CHLORIDE ION × 2 FME N-FORMYLMETHIONINE × 1 KIR KIRROMYCIN × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 NA SODIUM ION × 2 ZN ZINC ION × 2	ELECTRON MICROSCOPY cryo-EM buffer 50MM HEPES-KOH, 70MMNH4CL, 30MM KCL, 20MM MGCL2, 1MM DTT, 0.6MM SPERMINE, 0.4MM SPERMIDINE, 0.15MM KIRROMYCIN；pH 7.5；50MM HEPES-KOH, 70MMNH4CL, 30MM KCL, 20MM MGCL2, 1MM DTT, 0.6MM SPERMINE, 0.4MM SPERMIDINE, 0.15MM KIRROMYCIN cryo-EM vitrification conditions Cryogen ETHANE；LIQUIDE ETHANE	Resolution 2.90 Å
5AKA EM structure of ribosome-SRP-FtsY complex in closed state Deposited 2015-03-03	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 31 PDB declaration: 34-meric	Chain L 1–144（144 aa）	Not recorded	MG MAGNESIUM ION × 110	ELECTRON MICROSCOPY cryo-EM buffer 50MM HEPES-KOH, 100MM KOAC, 8MM MG(OAC)2, 500UG/ML CHLORAMPHENICO；pH 7.5；50MM HEPES-KOH, 100MM KOAC, 8MM MG(OAC)2, 500UG/ML CHLORAMPHENICO cryo-EM vitrification conditions Cryogen ETHANE；LIQUID ETHAN	Resolution 5.70 Å
5GAD RNC-SRP-SR complex early state Deposited 2015-11-24	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 33 PDB declaration: 37-meric	Chain M 1–144（144 aa）	Not recorded	MG MAGNESIUM ION × 433 ZN ZINC ION × 1 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.70 Å
5GAE RNC in complex with a translocating SecYEG Deposited 2015-11-25	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 33 PDB declaration: 36-meric	Chain M 1–144（144 aa）	Not recorded	MG MAGNESIUM ION × 323 ZN ZINC ION × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.33 Å
5GAF RNC in complex with SRP Deposited 2015-11-25	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 32 PDB declaration: 36-meric	Chain M 1–144（144 aa）	Not recorded	MG MAGNESIUM ION × 431 ZN ZINC ION × 1 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 4.30 Å
5GAG RNC in complex with SRP-SR in the closed state Deposited 2015-11-26	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 32 PDB declaration: 36-meric	Chain M 1–144（144 aa）	Not recorded	MG MAGNESIUM ION × 431 ZN ZINC ION × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.80 Å
5GAH RNC in complex with SRP with detached NG domain Deposited 2015-11-26	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 32 PDB declaration: 36-meric	Chain M 1–144（144 aa）	Not recorded	MG MAGNESIUM ION × 431 ZN ZINC ION × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.80 Å
5H5U Mechanistic insights into the alternative translation termination by ArfA and RF2 Deposited 2016-11-09	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 57-meric	Chain M 1–144（144 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.01 Å
5IQR Structure of RelA bound to the 70S ribosome Deposited 2016-03-11	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 59-meric	Chain L 1–144（144 aa）	Not recorded	MG MAGNESIUM ION × 299 ZN ZINC ION × 3 PAR PAROMOMYCIN × 1 MET METHIONINE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE；Grids were blotted for 5 s	Resolution 3.00 Å
5IT8 High-resolution structure of the Escherichia coli ribosome Deposited 2016-03-16	Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 53-meric	Chain DM 1–144（144 aa）	Not recorded	MG MAGNESIUM ION × 266 PG4 TETRAETHYLENE GLYCOL × 6 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 14 PUT 1,4-DIAMINOBUTANE × 16 ZN ZINC ION × 2 PEG DI(HYDROXYETHYL)ETHER × 11 EDO 1,2-ETHANEDIOL × 12 PGE TRIETHYLENE GLYCOL × 9 SPD SPERMIDINE × 4 1PE PENTAETHYLENE GLYCOL × 2 ACY ACETIC ACID × 3 GUN GUANINE × 1 TRS 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL × 1	X-RAY DIFFRACTION X-ray crystallization conditions MICROBATCH；pH 6.5；291 K；PEG8k, MPD	Resolution 3.12 Å R-free 0.220
5IT8 High-resolution structure of the Escherichia coli ribosome Deposited 2016-03-16	Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 52-meric	Chain CM 1–144（144 aa）	Not recorded	MG MAGNESIUM ION × 200 PG4 TETRAETHYLENE GLYCOL × 1 ZN ZINC ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions MICROBATCH；pH 6.5；291 K；PEG8k, MPD	Resolution 3.12 Å R-free 0.220
5J5B Structure of the WT E coli ribosome bound to tetracycline Deposited 2016-04-01	Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 53-meric	Chain DM 1–144（144 aa）	Not recorded	MG MAGNESIUM ION × 267 PG4 TETRAETHYLENE GLYCOL × 6 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 14 PUT 1,4-DIAMINOBUTANE × 16 TAC TETRACYCLINE × 1 ZN ZINC ION × 2 PEG DI(HYDROXYETHYL)ETHER × 11 EDO 1,2-ETHANEDIOL × 12 PGE TRIETHYLENE GLYCOL × 9 SPD SPERMIDINE × 4 1PE PENTAETHYLENE GLYCOL × 2 ACY ACETIC ACID × 3 GUN GUANINE × 1 TRS 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL × 1	X-RAY DIFFRACTION X-ray crystallization conditions MICROBATCH；291 K；PEG 8000, Spermidine, Putrescine, Ammonium Chloride, Magnesium Chloride, MES pH 6.5	Resolution 2.80 Å R-free 0.219
5J5B Structure of the WT E coli ribosome bound to tetracycline Deposited 2016-04-01	Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 52-meric	Chain CM 1–144（144 aa）	Not recorded	MG MAGNESIUM ION × 202 PG4 TETRAETHYLENE GLYCOL × 1 TAC TETRACYCLINE × 1 ZN ZINC ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions MICROBATCH；291 K；PEG 8000, Spermidine, Putrescine, Ammonium Chloride, Magnesium Chloride, MES pH 6.5	Resolution 2.80 Å R-free 0.219
5J7L Structure of the 70S E coli ribosome with the U1052G mutation in the 16S rRNA bound to tetracycline Deposited 2016-04-06	Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 53-meric	Chain DM 1–144（144 aa）	Not recorded	MG MAGNESIUM ION × 268 PG4 TETRAETHYLENE GLYCOL × 6 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 14 PUT 1,4-DIAMINOBUTANE × 16 TAC TETRACYCLINE × 2 ZN ZINC ION × 2 PEG DI(HYDROXYETHYL)ETHER × 11 EDO 1,2-ETHANEDIOL × 12 PGE TRIETHYLENE GLYCOL × 9 SPD SPERMIDINE × 4 1PE PENTAETHYLENE GLYCOL × 2 ACY ACETIC ACID × 3 GUN GUANINE × 1 TRS 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL × 1	X-RAY DIFFRACTION X-ray crystallization conditions MICROBATCH；291 K；PEG 8k, Spermidine, Putrescine, Ammonium Chloride, Magnesium Chloride, MES pH 6.5	Resolution 3.00 Å R-free 0.193
5J7L Structure of the 70S E coli ribosome with the U1052G mutation in the 16S rRNA bound to tetracycline Deposited 2016-04-06	Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 52-meric	Chain CM 1–144（144 aa）	Not recorded	MG MAGNESIUM ION × 204 PG4 TETRAETHYLENE GLYCOL × 1 TAC TETRACYCLINE × 2 ZN ZINC ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions MICROBATCH；291 K；PEG 8k, Spermidine, Putrescine, Ammonium Chloride, Magnesium Chloride, MES pH 6.5	Resolution 3.00 Å R-free 0.193
5J88 Structure of the E coli 70S ribosome with the U1060A mutation in 16S rRNA Deposited 2016-04-07	Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 53-meric	Chain DM 1–144（144 aa）	Not recorded	MG MAGNESIUM ION × 266 PG4 TETRAETHYLENE GLYCOL × 6 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 14 PUT 1,4-DIAMINOBUTANE × 16 ZN ZINC ION × 2 PEG DI(HYDROXYETHYL)ETHER × 11 EDO 1,2-ETHANEDIOL × 12 PGE TRIETHYLENE GLYCOL × 9 SPD SPERMIDINE × 4 1PE PENTAETHYLENE GLYCOL × 2 ACY ACETIC ACID × 3 GUN GUANINE × 1 TRS 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL × 1	X-RAY DIFFRACTION X-ray crystallization conditions MICROBATCH；291 K；PEG 8k, Spermidine, Putrescine, Ammonium Chloride, Magnesium Chloride, MES pH 6.5	Resolution 3.32 Å R-free 0.219
5J88 Structure of the E coli 70S ribosome with the U1060A mutation in 16S rRNA Deposited 2016-04-07	Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 52-meric	Chain CM 1–144（144 aa）	Not recorded	MG MAGNESIUM ION × 200 PG4 TETRAETHYLENE GLYCOL × 1 ZN ZINC ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions MICROBATCH；291 K；PEG 8k, Spermidine, Putrescine, Ammonium Chloride, Magnesium Chloride, MES pH 6.5	Resolution 3.32 Å R-free 0.219
5J8A Structure of the E coli 70S ribosome with the U1052G mutation in 16S rRNA bound to tigecycline Deposited 2016-04-07	Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 53-meric	Chain DM 1–144（144 aa）	Not recorded	MG MAGNESIUM ION × 268 PG4 TETRAETHYLENE GLYCOL × 6 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 14 PUT 1,4-DIAMINOBUTANE × 16 T1C TIGECYCLINE × 1 ZN ZINC ION × 2 PEG DI(HYDROXYETHYL)ETHER × 11 EDO 1,2-ETHANEDIOL × 12 PGE TRIETHYLENE GLYCOL × 9 SPD SPERMIDINE × 4 1PE PENTAETHYLENE GLYCOL × 2 ACY ACETIC ACID × 3 GUN GUANINE × 1 TRS 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL × 1	X-RAY DIFFRACTION X-ray crystallization conditions MICROBATCH；291 K；PEG 8k, Spermidine, Putrescine, Ammonium Chloride, MAgnesium Chloride, MPD, MES pH 6.5	Resolution 3.10 Å R-free 0.192
5J8A Structure of the E coli 70S ribosome with the U1052G mutation in 16S rRNA bound to tigecycline Deposited 2016-04-07	Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 52-meric	Chain CM 1–144（144 aa）	Not recorded	MG MAGNESIUM ION × 204 PG4 TETRAETHYLENE GLYCOL × 1 T1C TIGECYCLINE × 1 ZN ZINC ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions MICROBATCH；291 K；PEG 8k, Spermidine, Putrescine, Ammonium Chloride, MAgnesium Chloride, MPD, MES pH 6.5	Resolution 3.10 Å R-free 0.192
5J91 Structure of the Wild-type 70S E coli ribosome bound to Tigecycline Deposited 2016-04-08	Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 53-meric	Chain DM 1–144（144 aa）	Not recorded	MG MAGNESIUM ION × 267 PG4 TETRAETHYLENE GLYCOL × 6 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 14 PUT 1,4-DIAMINOBUTANE × 16 T1C TIGECYCLINE × 1 ZN ZINC ION × 2 PEG DI(HYDROXYETHYL)ETHER × 11 EDO 1,2-ETHANEDIOL × 12 PGE TRIETHYLENE GLYCOL × 9 SPD SPERMIDINE × 4 1PE PENTAETHYLENE GLYCOL × 2 ACY ACETIC ACID × 3 GUN GUANINE × 1 TRS 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL × 1	X-RAY DIFFRACTION X-ray crystallization conditions MICROBATCH；pH 6.5；291 K；PEG 8k, Spermidine, Putrescine, Ammonium Chloride, MAgnesium Chloride, MPD, MES pH 6.5	Resolution 2.96 Å R-free 0.221
5J91 Structure of the Wild-type 70S E coli ribosome bound to Tigecycline Deposited 2016-04-08	Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 52-meric	Chain CM 1–144（144 aa）	Not recorded	MG MAGNESIUM ION × 202 PG4 TETRAETHYLENE GLYCOL × 1 T1C TIGECYCLINE × 1 ZN ZINC ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions MICROBATCH；pH 6.5；291 K；PEG 8k, Spermidine, Putrescine, Ammonium Chloride, MAgnesium Chloride, MPD, MES pH 6.5	Resolution 2.96 Å R-free 0.221
5JC9 Structure of the Escherichia coli ribosome with the U1052G mutation in the 16S rRNA Deposited 2016-04-14	Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 53-meric	Chain DM 1–144（144 aa）	Not recorded	MG MAGNESIUM ION × 267 PG4 TETRAETHYLENE GLYCOL × 6 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 14 PUT 1,4-DIAMINOBUTANE × 16 ZN ZINC ION × 2 PEG DI(HYDROXYETHYL)ETHER × 11 EDO 1,2-ETHANEDIOL × 12 PGE TRIETHYLENE GLYCOL × 9 SPD SPERMIDINE × 4 1PE PENTAETHYLENE GLYCOL × 2 ACY ACETIC ACID × 3 GUN GUANINE × 1 TRS 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL × 1	X-RAY DIFFRACTION X-ray crystallization conditions MICROBATCH；pH 6.5；291 K；PEG 8k, Spermidine, Putrescine, Ammonium Chloride, MAgnesium Chloride, MPD, MES	Resolution 3.03 Å R-free 0.200
5JC9 Structure of the Escherichia coli ribosome with the U1052G mutation in the 16S rRNA Deposited 2016-04-14	Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 52-meric	Chain CM 1–144（144 aa）	Not recorded	MG MAGNESIUM ION × 202 PG4 TETRAETHYLENE GLYCOL × 1 ZN ZINC ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions MICROBATCH；pH 6.5；291 K；PEG 8k, Spermidine, Putrescine, Ammonium Chloride, MAgnesium Chloride, MPD, MES	Resolution 3.03 Å R-free 0.200
5JTE Cryo-EM structure of an ErmBL-stalled ribosome in complex with A-, P-, and E-tRNA Deposited 2016-05-09	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 58-meric	Chain BL 1–144（144 aa）	Not recorded	LYS LYSINE × 1 ERY ERYTHROMYCIN A × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.60 Å
5JU8 Cryo-EM structure of an ErmBL-stalled ribosome in complex with P-, and E-tRNA Deposited 2016-05-10	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric	Chain BL 1–144（144 aa）	Not recorded	ERY ERYTHROMYCIN A × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.60 Å
5KCR Cryo-EM structure of the Escherichia coli 70S ribosome in complex with antibiotic Avilamycin C, mRNA and P-site tRNA at 3.6A resolution Deposited 2016-06-06	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric	Chain 1P 1–144（144 aa）	Not recorded	6UQ (2R,3S,4R,6S)-4-hydroxy-6-{[(2R,3aR,4R,4'R,5'S,6S,6'R,7aR)-4'-hydroxy-6-{[(2S,3R,4R,5S,6R)-3-hydroxy-2-{[(2R,3S,4S,5S,6S)-4-hydroxy-6-({(2R,3aS,3a'R,6S,6'R,7R,7'R,7aR,7a'R)-7'-hydroxy-7'-[(1S)-1-hydroxyethyl]-6'-methyl-7-[(2-methylpropanoyl)oxy]octahydro-4H-2,4'-spirobi[[1,3]dioxolo[4,5-c]pyran]-6-yl}oxy)-5-methoxy-2-(methoxymethyl)tetrahydro-2H-pyran-3-yl]oxy}-5-methoxy-6-methyltetrahydro-2H-pyran-4-yl]oxy}-4,6',7a-trimethyloctahydro-4H-spiro[1,3-dioxolo[4,5-c]pyran-2,2'-pyran]-5'-yl]oxy}-2-methyltetrahydro-2H-pyran-3-yl 3,5-dichloro-4-hydroxy-2-methoxy-6-methylbenzoate (non-preferred name) × 1 ZN ZINC ION × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE X-ray crystallization conditions pH 7.4	Resolution 3.60 Å
5KCS Cryo-EM structure of the Escherichia coli 70S ribosome in complex with antibiotic Evernimycin, mRNA, TetM and P-site tRNA at 3.9A resolution Deposited 2016-06-06	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 53 PDB declaration: 58-meric	Chain 1P 1–144（144 aa）	Not recorded	EVN (2R,3R,4R,6S)-6-{[(2R,3aR,4R,4'R,5'S,6S,6'R,7S,7aR)-6-{[(2S,3R,4R,5S,6R)-2-{[(2R,3S,4S,5S,6S)-6-({(2R,3aS,3a'R,6S,7R,7' R,7aS,7a'S)-7'-[(2,4-dihydroxy-6-methylbenzoyl)oxy]-7-hydroxyoctahydro-4H-2,4'-spirobi[[1,3]dioxolo[4,5-c]pyran]-6-yl}ox y)-4-hydroxy-5-methoxy-2-(methoxymethyl)tetrahydro-2H-pyran-3-yl]oxy}-3-hydroxy-5-methoxy-6-methyltetrahydro-2H-pyran-4- yl]oxy}-4',7-dihydroxy-4,6',7a-trimethyloctahydro-4H-spiro[1,3-dioxolo[4,5-c]pyran-2,2'-pyran]-5'-yl]oxy}-4-{[(2R,4S,5R, 6S)-5-methoxy-4,6-dimethyl-4-nitrotetrahydro-2H-pyran-2-yl]oxy}-2-methyltetrahydro-2H-pyran-3-yl 3,5-dichloro-4-hydroxy-2-methoxy-6-methylbenzoate (non-preferred name) × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.90 Å
5KPS Structure of RelA bound to ribosome in absence of A/R tRNA (Structure I) Deposited 2016-07-05	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 58-meric	Chain L 1–144（144 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE；Apply 2 uL sample and blot 4 seconds before plunging into liquid ethane (GATAN CRYOPLUNGE 3).	Resolution 3.90 Å
5KPV Structure of RelA bound to ribosome in presence of A/R tRNA (Structure II) Deposited 2016-07-05	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 59-meric	Chain K 1–144（144 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE；Apply 2 uL sample and blot 4 seconds before plunging into liquid ethane (GATAN CRYOPLUNGE 3).	Resolution 4.10 Å
5KPW Structure of RelA bound to ribosome in presence of A/R tRNA (Structure III) Deposited 2016-07-05	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 59-meric	Chain K 1–144（144 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE；Apply 2 uL sample and blot 4 seconds before plunging into liquid ethane (GATAN CRYOPLUNGE 3).	Resolution 3.90 Å
5KPX Structure of RelA bound to ribosome in presence of A/R tRNA (Structure IV) Deposited 2016-07-05	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 59-meric	Chain K 1–144（144 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE；Apply 2 uL sample and blot 4 seconds before plunging into liquid ethane (GATAN CRYOPLUNGE 3).	Resolution 3.90 Å
5L3P Cryo-EM structure of stringent response factor RelA bound to ErmCL-stalled ribosome complex Deposited 2016-05-24	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 58-meric	Chain P 1–144（144 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.70 Å
5LZA Structure of the 70S ribosome with SECIS-mRNA and P-site tRNA (Initial complex, IC) Deposited 2016-09-29	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric	Chain L 2–144（143 aa）	Not recorded	ZN ZINC ION × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5；50 mM Hepes-KOH, pH 7.5, 70 mM NH4Cl, 30 mM KCl, 7 mM MgCl2, 0.6mM spermine, 0.4mM spermidine, 2 mM DTT cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.60 Å
5LZB Structure of SelB-Sec-tRNASec bound to the 70S ribosome in the initial binding state (IB) Deposited 2016-09-29	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 58-meric	Chain L 2–144（143 aa）	Not recorded	FME N-FORMYLMETHIONINE × 1 SEC SELENOCYSTEINE × 1 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 ZN ZINC ION × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5；50 mM Hepes-KOH, pH 7.5, 70 mM NH4Cl, 30 mM KCl, 7 mM MgCl2, 0.6mM spermine, 0.4mM spermidine, 2 mM DTT cryo-EM vitrification conditions Cryogen ETHANE	Resolution 5.30 Å
5LZC Structure of SelB-Sec-tRNASec bound to the 70S ribosome in the codon reading state (CR) Deposited 2016-09-29	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 58-meric	Chain L 2–144（143 aa）	Not recorded	FME N-FORMYLMETHIONINE × 1 SEC SELENOCYSTEINE × 1 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 ZN ZINC ION × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5；50 mM Hepes-KOH, pH 7.5, 70 mM NH4Cl, 30 mM KCl, 7 mM MgCl2, 0.6mM spermine, 0.4mM spermidine, 2 mM DTT cryo-EM vitrification conditions Cryogen ETHANE	Resolution 4.80 Å
5LZD Structure of SelB-Sec-tRNASec bound to the 70S ribosome in the GTPase activated state (GA) Deposited 2016-09-29	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 58-meric	Chain L 2–144（143 aa）	Not recorded	CL CHLORIDE ION × 1 MG MAGNESIUM ION × 147 FME N-FORMYLMETHIONINE × 1 SEC SELENOCYSTEINE × 1 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 ZN ZINC ION × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5；50 mM Hepes-KOH, pH 7.5, 70 mM NH4Cl, 30 mM KCl, 7 mM MgCl2, 0.6mM spermine, 0.4mM spermidine, 2 mM DTT cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.40 Å
5LZE Structure of the 70S ribosome with Sec-tRNASec in the classical pre-translocation state (C) Deposited 2016-09-29	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 57-meric	Chain L 2–144（143 aa）	Not recorded	ZN ZINC ION × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5；50 mM Hepes-KOH, pH 7.5, 70 mM NH4Cl, 30 mM KCl, 7 mM MgCl2, 0.6 mM spermine, 0.4 mM spermidine, 2 mM DTT cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.50 Å
5LZF Structure of the 70S ribosome with fMetSec-tRNASec in the hybrid pre-translocation state (H) Deposited 2016-09-29	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric	Chain L 2–144（143 aa）	Not recorded	ZN ZINC ION × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5；50 mM Hepes-KOH, pH 7.5, 70 mM NH4Cl, 30 mM KCl, 7 mM MgCl2, 0.6mM spermine, 0.4mM spermidine, 2 mM DTT cryo-EM vitrification conditions Cryogen ETHANE	Resolution 4.60 Å
5MDV Structure of ArfA and RF2 bound to the 70S ribosome (accommodated state) Deposited 2016-11-13	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 53 PDB declaration: 58-meric	Chain L 1–144（144 aa）	Not recorded	MG MAGNESIUM ION × 440 FME N-FORMYLMETHIONINE × 1 ZN ZINC ION × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.97 Å
5MDW Structure of ArfA(A18T) and RF2 bound to the 70S ribosome (pre-accommodated state) Deposited 2016-11-13	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 53 PDB declaration: 58-meric	Chain L 1–144（144 aa）	Not recorded	MG MAGNESIUM ION × 438 FME N-FORMYLMETHIONINE × 1 ZN ZINC ION × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.06 Å
5MDY Structure of ArfA and TtRF2 bound to the 70S ribosome (pre-accommodated state) Deposited 2016-11-13	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 53 PDB declaration: 62-meric	Chain L 1–144（144 aa）	Not recorded	MG MAGNESIUM ION × 450 FME N-FORMYLMETHIONINE × 1 ZN ZINC ION × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.35 Å
5MDZ Structure of the 70S ribosome (empty A site) Deposited 2016-11-13	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 56-meric	Chain L 1–144（144 aa）	Not recorded	MG MAGNESIUM ION × 437 FME N-FORMYLMETHIONINE × 1 ZN ZINC ION × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.10 Å
5MGP Structural basis for ArfA-RF2 mediated translation termination on stop-codon lacking mRNAs Deposited 2016-11-21	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 56-meric	Chain L 2–144（143 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.2 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.10 Å
5NCO Quaternary complex between SRP, SR, and SecYEG bound to the translating ribosome Deposited 2017-03-06	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 36 PDB declaration: 40-meric	Chain M 1–144（144 aa）	Not recorded	ZN ZINC ION × 1 ALF TETRAFLUOROALUMINATE ION × 2 MG MAGNESIUM ION × 2 GDP GUANOSINE-5'-DIPHOSPHATE × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 4.80 Å
5NP6 70S structure prior to bypassing Deposited 2017-04-13	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 57-meric	Chain j 2–144（143 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.60 Å
5NWY 2.9 A cryo-EM structure of VemP-stalled ribosome-nascent chain complex Deposited 2017-05-08	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 56-meric	Chain Y 1–144（144 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.2 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.90 Å
5O2R Cryo-EM structure of the proline-rich antimicrobial peptide Api137 bound to the terminating ribosome Deposited 2017-05-22	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 53 PDB declaration: 58-meric	Chain L 2–144（143 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.40 Å
5U4I Structural Basis of Co-translational Quality Control by ArfA and RF2 Bound to Ribosome Deposited 2016-12-04	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 57-meric	Chain M 1–144（144 aa）	Not recorded	MG MAGNESIUM ION × 124 ZN ZINC ION × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.50 Å
5UYK 70S ribosome bound with cognate ternary complex not base-paired to A site codon (Structure I) Deposited 2017-02-24	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 53 PDB declaration: 60-meric	Chain 14 2–144（143 aa）	Not recorded	GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE；2 uL of complex was applied to each grid. After a 10-second incubation, the grids were blotted for 2 to 4 seconds.	Resolution 3.90 Å
5UYL 70S ribosome bound with cognate ternary complex base-paired to A site codon (Structure II) Deposited 2017-02-24	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 53 PDB declaration: 60-meric	Chain 14 2–144（143 aa）	Not recorded	FME N-FORMYLMETHIONINE × 1 PHE PHENYLALANINE × 1 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE；2 uL of complex was applied to each grid. After a 10-second incubation, the grids were blotted for 2 to 4 seconds.	Resolution 3.60 Å
5UYM 70S ribosome bound with cognate ternary complex base-paired to A site codon, closed 30S (Structure III) Deposited 2017-02-24	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 53 PDB declaration: 60-meric	Chain 14 2–144（143 aa）	Not recorded	MG MAGNESIUM ION × 383 ZN ZINC ION × 2 FME N-FORMYLMETHIONINE × 1 PHE PHENYLALANINE × 1 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE；2 uL of complex was applied to each grid. After a 10-second incubation, the grids were blotted for 2 to 4 seconds.	Resolution 3.20 Å
5UYN 70S ribosome bound with near-cognate ternary complex not base-paired to A site codon (Structure I-nc) Deposited 2017-02-24	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 53 PDB declaration: 60-meric	Chain 14 2–144（143 aa）	Not recorded	GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE；2 uL of complex was applied to each grid. After a 10-second incubation, the grids were blotted for 2 to 4 seconds.	Resolution 4.00 Å
5UYP 70S ribosome bound with near-cognate ternary complex base-paired to A site codon, open 30S (Structure II-nc) Deposited 2017-02-24	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 53 PDB declaration: 60-meric	Chain 14 2–144（143 aa）	Not recorded	FME N-FORMYLMETHIONINE × 1 LYS LYSINE × 1 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE；2 uL of complex was applied to each grid. After a 10-second incubation, the grids were blotted for 2 to 4 seconds.	Resolution 3.90 Å
5UYQ 70S ribosome bound with near-cognate ternary complex base-paired to A site codon, closed 30S (Structure III-nc) Deposited 2017-02-24	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 53 PDB declaration: 60-meric	Chain 14 2–144（143 aa）	Not recorded	FME N-FORMYLMETHIONINE × 1 LYS LYSINE × 1 MG MAGNESIUM ION × 1 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE；2 uL of complex was applied to each grid. After a 10-second incubation, the grids were blotted for 2 to 4 seconds.	Resolution 3.80 Å
5WDT 70S ribosome-EF-Tu H84A complex with GppNHp Deposited 2017-07-06	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 59-meric	Chain L 1–143（143 aa）	Not recorded	FME N-FORMYLMETHIONINE × 1 MG MAGNESIUM ION × 1904 K POTASSIUM ION × 5 PHE PHENYLALANINE × 1 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE；5 second blot time, no wait time	Resolution 3.00 Å
5WE4 70S ribosome-EF-Tu wt complex with GppNHp Deposited 2017-07-07	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 59-meric	Chain L 1–143（143 aa）	Not recorded	MG MAGNESIUM ION × 1618 K POTASSIUM ION × 1 FME N-FORMYLMETHIONINE × 1 PHE PHENYLALANINE × 1 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE；5 second blot time, no wait time	Resolution 3.10 Å
5WE6 70S ribosome-EF-Tu H84A complex with GTP and cognate tRNA Deposited 2017-07-07	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 59-meric	Chain L 2–143（142 aa）	Not recorded	FME N-FORMYLMETHIONINE × 1 MG MAGNESIUM ION × 662 K POTASSIUM ION × 8 PHE PHENYLALANINE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE；5 second blot time, no wait time	Resolution 3.40 Å
5WFK 70S ribosome-EF-Tu H84A complex with GTP and near-cognate tRNA (Complex C3) Deposited 2017-07-12	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 59-meric	Chain L 1–143（143 aa）	Not recorded	FME N-FORMYLMETHIONINE × 1 MG MAGNESIUM ION × 1413 K POTASSIUM ION × 1 PHE PHENYLALANINE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE；5 second blot time, no wait time	Resolution 3.40 Å
6BU8 70S ribosome with S1 domains 1 and 2 (Class 1) Deposited 2017-12-08	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 53 PDB declaration: 60-meric	Chain 14 2–144（143 aa）	Not recorded	FME N-FORMYLMETHIONINE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE；2 uL of complex was applied to each grid. After a 10-second incubation, the grids were blotted for 2 to 4 seconds.	Resolution 3.50 Å
6BY1 E. coli pH03H9 complex Deposited 2017-12-19	Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 57-meric	Chain CL 2–144（143 aa）	Not recorded	MG MAGNESIUM ION × 225 ZN ZINC ION × 2	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；289 K；PEG 2000, PEP629, potassium thiocyanate, tris acetate	Resolution 3.94 Å R-free 0.269
6BY1 E. coli pH03H9 complex Deposited 2017-12-19	Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric	Chain DL 2–144（143 aa）	Not recorded	MG MAGNESIUM ION × 220 ZN ZINC ION × 2	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；289 K；PEG 2000, PEP629, potassium thiocyanate, tris acetate	Resolution 3.94 Å R-free 0.269
6C4I Conformation of methylated GGQ in the peptidyl transferase center during translation termination Deposited 2018-01-12	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 53 PDB declaration: 59-meric	Chain M 1–144（144 aa）	Not recorded	MG MAGNESIUM ION × 317 ZN ZINC ION × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE；Grids were blotted for 3.5 seconds.	Resolution 3.24 Å
6DNC E.coli RF1 bound to E.coli 70S ribosome in response to UAU sense A-site codon Deposited 2018-06-06	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 53 PDB declaration: 59-meric	Chain P 1–144（144 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7；20 mM Tris-HCl, 100 mM NH4Cl, 20.5 mM MgCl2, 0.5 mM EDTA, 6 mM beta-mercaptoethanol cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.70 Å
6ENF Cryo-EM structure of a polyproline-stalled ribosome in the absence of EF-P Deposited 2017-10-04	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 53-meric	Chain L 2–144（143 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.20 Å
6ENJ Polyproline-stalled ribosome in the presence of A+P site tRNA and elongation-factor P (EF-P) Deposited 2017-10-05	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric	Chain L 2–144（143 aa）	Not recorded	PRO PROLINE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.70 Å
6ENU Polyproline-stalled ribosome in the presence of elongation-factor P (EF-P) Deposited 2017-10-06	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 56-meric	Chain L 2–144（143 aa）	Not recorded	PRO PROLINE × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.10 Å
6GBZ 50S ribosomal subunit assembly intermediate state 5 Deposited 2018-04-16	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 27 PDB declaration: 29-meric	Chain L 2–144（143 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.80 Å
6GC0 50S ribosomal subunit assembly intermediate state 4 Deposited 2018-04-16	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 26 PDB declaration: 28-meric	Chain L 2–144（143 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.80 Å
6GC4 50S ribosomal subunit assembly intermediate state 3 Deposited 2018-04-17	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 24 PDB declaration: 26-meric	Chain L 2–144（143 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 4.30 Å
6GC6 50S ribosomal subunit assembly intermediate state 2 Deposited 2018-04-17	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 18 PDB declaration: nonadecameric	Chain L 2–144（143 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 4.30 Å
6GC7 50S ribosomal subunit assembly intermediate state 1 Deposited 2018-04-17	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 16 PDB declaration: heptadecameric	Chain L 2–144（143 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 4.30 Å
6GC8 50S ribosomal subunit assembly intermediate - 50S rec* Deposited 2018-04-17	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 27 PDB declaration: 29-meric	Chain L 2–144（143 aa）	Not recorded	ZN ZINC ION × 1 MG MAGNESIUM ION × 5	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.80 Å
6GWT Cryo-EM structure of an E. coli 70S ribosome in complex with RF3-GDPCP, RF1(GAQ) and Pint-tRNA (State I) Deposited 2018-06-25	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 53 PDB declaration: 58-meric	Chain L 2–144（143 aa）	Not recorded	GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4；Solutions were preapred fresh and filtered previous to usage. cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.80 Å
6GXM Cryo-EM structure of an E. coli 70S ribosome in complex with RF3-GDPCP, RF1(GAQ) and Pint-tRNA (State II) Deposited 2018-06-27	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 53 PDB declaration: 58-meric	Chain L 2–144（143 aa）	Not recorded	GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4；Solutions were preapred fresh and filtered previous to usage. cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.80 Å
6GXN Cryo-EM structure of an E. coli 70S ribosome in complex with RF3-GDPCP, RF1(GAQ) and Pint-tRNA (State III) Deposited 2018-06-27	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 53 PDB declaration: 58-meric	Chain L 2–144（143 aa）	Not recorded	GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4；Solutions were preapred fresh and filtered previous to usage. cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.90 Å
6GXO Cryo-EM structure of a rotated E. coli 70S ribosome in complex with RF3-GDPCP, RF1(GAQ) and P/E-tRNA (State IV) Deposited 2018-06-27	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 53 PDB declaration: 58-meric	Chain L 2–144（143 aa）	Not recorded	GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4；Solutions were preapred fresh and filtered previous to usage. cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.90 Å
6GXP Cryo-EM structure of a rotated E. coli 70S ribosome in complex with RF3-GDPCP(RF3-only) Deposited 2018-06-27	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 55-meric	Chain L 2–144（143 aa）	Not recorded	GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4；Solutions were preapred fresh and filtered previous to usage. cryo-EM vitrification conditions Cryogen ETHANE	Resolution 4.40 Å
6H4N Structure of a hibernating 100S ribosome reveals an inactive conformation of the ribosomal protein S1 - 70S Hibernating E. coli Ribosome Deposited 2018-07-22	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 57-meric	Chain L 2–144（143 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5；B100S buffer (25 mM Hepes pH 7.5, 100 mM KOAc, 15 mM Mg(OAc)2, 1 mM dithiothreitol) cryo-EM vitrification conditions Cryogen ETHANE-PROPANE	Resolution 3.00 Å
6H58 Structure of a hibernating 100S ribosome reveals an inactive conformation of the ribosomal protein S1 - Full 100S Hibernating E. coli Ribosome Deposited 2018-07-24	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 104 PDB declaration: 112-meric	Chain L 2–144（143 aa） Chain LL 2–144（143 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE-PROPANE	Resolution 7.90 Å
6HRM E. coli 70S d2d8 stapled ribosome Deposited 2018-09-27	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 53-meric	Chain L 1–144（144 aa）	Not recorded	MG MAGNESIUM ION × 436 ZN ZINC ION × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.96 Å
6I0Y TnaC-stalled ribosome complex with the titin I27 domain folding close to the ribosomal exit tunnel Deposited 2018-10-26	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 33 PDB declaration: 36-meric	Chain L 2–144（143 aa）	Not recorded	MG MAGNESIUM ION × 143 ZN ZINC ION × 1 TRP TRYPTOPHAN × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.20 Å
6I7V Ribosomal protein paralogs bL31 and bL36 Deposited 2018-11-19	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 99 PDB declaration: 105-meric	Chain CM 1–144（144 aa） Chain DM 1–144（144 aa）	Not recorded	MG MAGNESIUM ION × 453 PGE TRIETHYLENE GLYCOL × 8 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 13 PG4 TETRAETHYLENE GLYCOL × 5 SPD SPERMIDINE × 3 PUT 1,4-DIAMINOBUTANE × 7 1PE PENTAETHYLENE GLYCOL × 2 ACY ACETIC ACID × 3 PEG DI(HYDROXYETHYL)ETHER × 9 EDO 1,2-ETHANEDIOL × 12 GUN GUANINE × 1	X-RAY DIFFRACTION X-ray crystallization conditions MICROBATCH；pH 6.5；291 K；PEG8k, MPD, KSCN	Resolution 2.90 Å R-free 0.255
6OFX Non-rotated ribosome (Structure I) Deposited 2019-04-01	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 54-meric	Chain l 2–144（143 aa）	Not recorded	FME N-FORMYLMETHIONINE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.30 Å
6OG7 70S termination complex with RF2 bound to the UGA codon. Non-rotated ribosome with RF2 bound (Structure II) Deposited 2019-04-01	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric	Chain l 2–144（143 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.30 Å
6Q97 Structure of tmRNA SmpB bound in A site of E. coli 70S ribosome Deposited 2018-12-17	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 53 PDB declaration: 60-meric	Chain L 1–144（144 aa）	Not recorded	MG MAGNESIUM ION × 439 ZN ZINC ION × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.90 Å
6Q98 Structure of tmRNA SmpB bound in P site of E. coli 70S ribosome Deposited 2018-12-17	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 53 PDB declaration: 57-meric	Chain L 1–144（144 aa）	Not recorded	MG MAGNESIUM ION × 429 ZN ZINC ION × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 4.30 Å
6Q9A Structure of tmRNA SmpB bound past E site of E. coli 70S ribosome Deposited 2018-12-17	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 53 PDB declaration: 58-meric	Chain L 1–143（143 aa）	Not recorded	MG MAGNESIUM ION × 437 ZN ZINC ION × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.70 Å
6QUL Structure of a bacterial 50S ribosomal subunit in complex with the novel quinoxolidinone antibiotic cadazolid Deposited 2019-02-27	Different oligomeric state Different ligand/ion Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 28 PDB declaration: 31-meric	Chain M 1–144（144 aa）	Not recorded	FME N-FORMYLMETHIONINE × 1 MG MAGNESIUM ION × 382 AMP ADENOSINE MONOPHOSPHATE × 1 JJH cadazolid × 1 ZN ZINC ION × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE-PROPANE	Resolution 3.00 Å
6S0K Ribosome nascent chain in complex with SecA Deposited 2019-06-17	Different oligomeric state Different ligand/ion Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 32 PDB declaration: 35-meric	Chain M 1–144（144 aa）	Not recorded	MG MAGNESIUM ION × 68 ZN ZINC ION × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE-PROPANE	Resolution 3.10 Å
6SZS Release factor-dependent ribosome rescue by BrfA in the Gram-positive bacterium Bacillus subtilis Deposited 2019-10-02	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 56-meric	Chain L 1–144（144 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.2 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.06 Å
6TBV Cryo-EM structure of an Escherichia coli ribosome-SpeFL complex stalled in response to L-ornithine (Replicate 2) Deposited 2019-11-04	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric	Chain L151 1–144（144 aa）	Not recorded	MG MAGNESIUM ION × 339 K POTASSIUM ION × 147 UNX UNKNOWN LIGAND × 1201 ZN ZINC ION × 3 ORN L-ornithine × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.70 Å
6TC3 Cryo-EM structure of an Escherichia coli ribosome-SpeFL complex stalled in response to L-ornithine (Replicate 1) Deposited 2019-11-05	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric	Chain L151 1–144（144 aa）	Not recorded	MG MAGNESIUM ION × 339 K POTASSIUM ION × 147 UNX UNKNOWN LIGAND × 1201 ZN ZINC ION × 3 ORN L-ornithine × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.70 Å
6VWL 70S ribosome bound to HIV frameshifting stem-loop (FSS) and P/E tRNA (rotated conformation) Deposited 2020-02-20	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 53-meric	Chain J 1–144（144 aa）	Not recorded	MG MAGNESIUM ION × 34	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.10 Å
6VWM 70S ribosome bound to HIV frameshifting stem-loop (FSS) and P-site tRNA (non-rotated conformation, Structure I) Deposited 2020-02-20	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 53-meric	Chain J 1–144（144 aa）	Not recorded	MG MAGNESIUM ION × 53	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.40 Å
6VWN 70S ribosome bound to HIV frameshifting stem-loop (FSS) and P-site tRNA (non-rotated conformation, Structure II) Deposited 2020-02-20	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 53-meric	Chain J 1–144（144 aa）	Not recorded	MG MAGNESIUM ION × 55	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.40 Å
6WD6 Cryo-EM of elongating ribosome with EF-Tu*GTP elucidates tRNA proofreading (Cognate Structure II-C2) Deposited 2020-03-31	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 59-meric	Chain l 2–144（143 aa）	Not recorded	FME N-FORMYLMETHIONINE × 1 PHE PHENYLALANINE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.70 Å
6WDB Cryo-EM of elongating ribosome with EF-Tu*GTP elucidates tRNA proofreading (Cognate Structure IV-A) Deposited 2020-03-31	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 58-meric	Chain l 2–144（143 aa）	Not recorded	FME N-FORMYLMETHIONINE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 4.00 Å
6WDC Cryo-EM of elongating ribosome with EF-Tu*GTP elucidates tRNA proofreading (Cognate Structure IV-B) Deposited 2020-03-31	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 58-meric	Chain l 2–144（143 aa）	Not recorded	FME N-FORMYLMETHIONINE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 4.20 Å
6WDD Cryo-EM of elongating ribosome with EF-Tu*GTP elucidates tRNA proofreading (Cognate Structure V-A) Deposited 2020-03-31	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 58-meric	Chain l 2–144（143 aa）	Not recorded	FME N-FORMYLMETHIONINE × 1 PHE PHENYLALANINE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.20 Å
6WDE Cryo-EM of elongating ribosome with EF-Tu*GTP elucidates tRNA proofreading (Cognate Structure V-B) Deposited 2020-03-31	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 58-meric	Chain l 2–144（143 aa）	Not recorded	PHE PHENYLALANINE × 1 MET METHIONINE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.00 Å
6WDF Cryo-EM of elongating ribosome with EF-Tu*GTP elucidates tRNA proofreading (Cognate Structure VI-A) Deposited 2020-03-31	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 57-meric	Chain l 2–144（143 aa）	Not recorded	PHE PHENYLALANINE × 1 FME N-FORMYLMETHIONINE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.30 Å
6WDG Cryo-EM of elongating ribosome with EF-Tu*GTP elucidates tRNA proofreading (Cognate Structure VI-B) Deposited 2020-03-31	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 57-meric	Chain l 2–144（143 aa）	Not recorded	PHE PHENYLALANINE × 1 FME N-FORMYLMETHIONINE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.30 Å
6WDH Cryo-EM of elongating ribosome with EF-Tu*GTP elucidates tRNA proofreading (Non-cognate Structure IV-B1) Deposited 2020-03-31	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 58-meric	Chain l 2–144（143 aa）	Not recorded	FME N-FORMYLMETHIONINE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 4.30 Å
6WDI Cryo-EM of elongating ribosome with EF-Tu*GTP elucidates tRNA proofreading (Non-cognate Structure IV-B2) Deposited 2020-03-31	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 58-meric	Chain l 2–144（143 aa）	Not recorded	FME N-FORMYLMETHIONINE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 4.00 Å
6WDJ Cryo-EM of elongating ribosome with EF-Tu*GTP elucidates tRNA proofreading (Non-cognate Structure V-A1) Deposited 2020-03-31	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 58-meric	Chain l 2–144（143 aa）	Not recorded	FME N-FORMYLMETHIONINE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.70 Å
6WDK Cryo-EM of elongating ribosome with EF-Tu*GTP elucidates tRNA proofreading (Non-cognate Structure V-A2) Deposited 2020-03-31	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 58-meric	Chain l 2–144（143 aa）	Not recorded	FME N-FORMYLMETHIONINE × 1 PHE PHENYLALANINE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.60 Å
6WDL Cryo-EM of elongating ribosome with EF-Tu*GTP elucidates tRNA proofreading (Non-cognate Structure V-B1) Deposited 2020-03-31	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 58-meric	Chain l 2–144（143 aa）	Not recorded	FME N-FORMYLMETHIONINE × 1 PHE PHENYLALANINE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.70 Å
6WDM Cryo-EM of elongating ribosome with EF-Tu*GTP elucidates tRNA proofreading (Non-cognate Structure V-B2) Deposited 2020-03-31	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 58-meric	Chain l 2–144（143 aa）	Not recorded	PHE PHENYLALANINE × 1 FME N-FORMYLMETHIONINE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.60 Å
6WNT 50S ribosomal subunit without free 5S rRNA and perturbed PTC Deposited 2020-04-23	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 30 PDB declaration: 31-meric	Chain l 2–144（143 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.10 Å
6WNV 70S ribosome without free 5S rRNA and with a perturbed PTC Deposited 2020-04-23	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 52-meric	Chain l 2–144（143 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.50 Å
6WNW Active 70S ribosome without free 5S rRNA and bound with A- and P- tRNA Deposited 2020-04-23	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 57-meric	Chain l 2–144（143 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.20 Å
6XZ7 E. coli 50S ribosomal subunit in complex with dirithromycin, fMet-Phe-tRNA(Phe) and deacylated tRNA(iMet). Deposited 2020-02-03	Different oligomeric state Different ligand/ion Different experimental conditions	Assembly 1 Protein–RNA Heteromer；Protein × 29 PDB declaration: 33-meric	Chain L 1–144（144 aa）	Not recorded	MG MAGNESIUM ION × 178 DI0 Dirithromycin × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.10 Å
6XZA E. coli 70S ribosome in complex with dirithromycin, and deacylated tRNA(iMet) (focused classification). Deposited 2020-02-03	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric	Chain L2 1–144（144 aa）	Not recorded	DI0 Dirithromycin × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.66 Å
6XZB E. coli 70S ribosome in complex with dirithromycin, fMet-Phe-tRNA(Phe) and deacylated tRNA(iMet) (focused classification). Deposited 2020-02-03	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 54-meric	Chain L2 1–144（144 aa）	Not recorded	DI0 Dirithromycin × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.54 Å
6Y69 Cryo-EM structure of an Escherichia coli 70S ribosome in complex with antibiotic TetracenomycinX Deposited 2020-02-26	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 54-meric	Chain L 2–144（143 aa）	Not recorded	OCW Tetracenomycin X × 1 MG MAGNESIUM ION × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.86 Å
6YSR Structure of the P+9 stalled ribosome complex Deposited 2020-04-23	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 57-meric	Chain L 1–144（144 aa）	Not recorded	MG MAGNESIUM ION × 332 ZN ZINC ION × 2 CL CHLORIDE ION × 1 NA SODIUM ION × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4；50 mM HEPES, 30 mM KCl, 7 mM MgCl2 cryo-EM vitrification conditions Cryogen ETHANE；Manual blotting & plunge-freezing	Resolution 3.10 Å
6YSS Structure of the P+9 ArfB-ribosome complex in the post-hydrolysis state Deposited 2020-04-23	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 53 PDB declaration: 58-meric	Chain L 1–144（144 aa）	Not recorded	MG MAGNESIUM ION × 387 ZN ZINC ION × 2 NA SODIUM ION × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4；50 mM HEPES, 30 mM KCl, 7 mM MgCl2 cryo-EM vitrification conditions Cryogen ETHANE；Manual blotting & plunge-freezing	Resolution 2.60 Å
6YST Structure of the P+9 ArfB-ribosome complex with P/E hybrid tRNA in the post-hydrolysis state Deposited 2020-04-23	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 53 PDB declaration: 58-meric	Chain L 1–144（144 aa）	Not recorded	MG MAGNESIUM ION × 253 ZN ZINC ION × 2 NA SODIUM ION × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4；50 mM HEPES, 30 mM KCl, 7 mM MgCl2 cryo-EM vitrification conditions Cryogen ETHANE；Manual blotting & plunge-freezing	Resolution 3.20 Å
6YSU Structure of the P+0 ArfB-ribosome complex in the post-hydrolysis state Deposited 2020-04-23	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 57-meric	Chain L 1–144（144 aa）	Not recorded	MG MAGNESIUM ION × 356 ZN ZINC ION × 1 NA SODIUM ION × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4；50 mM HEPES, 30 mM KCl, 7 mM MgCl2, 0.1 % w/v DDM cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.70 Å
6ZTJ E. coli 70S-RNAP expressome complex in NusG-coupled state (38 nt intervening mRNA) Deposited 2020-07-20	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Other combination Heteromer；Protein × 58 PDB declaration: 66-meric	Chain BM 1–144（144 aa）	Not recorded	MG MAGNESIUM ION × 479 PHE PHENYLALANINE × 1 ZN ZINC ION × 4	ELECTRON MICROSCOPY cryo-EM buffer pH 8 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.40 Å
6ZTM E. coli 70S-RNAP expressome complex in collided state without NusG Deposited 2020-07-20	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Other combination Heteromer；Protein × 52 PDB declaration: 60-meric	Chain BM 1–144（144 aa）	Not recorded	MG MAGNESIUM ION × 421 PHE PHENYLALANINE × 1 ZN ZINC ION × 3	ELECTRON MICROSCOPY cryo-EM buffer pH 8 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.30 Å
6ZTN E. coli 70S-RNAP expressome complex in NusG-coupled state (42 nt intervening mRNA) Deposited 2020-07-20	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Other combination Heteromer；Protein × 54 PDB declaration: 63-meric	Chain BM 1–144（144 aa）	Not recorded	MG MAGNESIUM ION × 487 PHE PHENYLALANINE × 1 ZN ZINC ION × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 8 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.90 Å
6ZTO E. coli 70S-RNAP expressome complex in uncoupled state 1 Deposited 2020-07-20	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Other combination Heteromer；Protein × 54 PDB declaration: 62-meric	Chain BM 1–144（144 aa）	Not recorded	MG MAGNESIUM ION × 487 PHE PHENYLALANINE × 1 ZN ZINC ION × 4	ELECTRON MICROSCOPY cryo-EM buffer pH 8 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.00 Å
6ZTP E. coli 70S-RNAP expressome complex in uncoupled state 6 Deposited 2020-07-20	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Other combination Heteromer；Protein × 54 PDB declaration: 62-meric	Chain BM 1–144（144 aa）	Not recorded	MG MAGNESIUM ION × 487 PHE PHENYLALANINE × 1 ZN ZINC ION × 4	ELECTRON MICROSCOPY cryo-EM buffer pH 8 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.00 Å
6ZU1 E. coli 70S-RNAP expressome complex in uncoupled state 2 Deposited 2020-07-21	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Other combination Heteromer；Protein × 54 PDB declaration: 62-meric	Chain BM 1–144（144 aa）	Not recorded	MG MAGNESIUM ION × 487 PHE PHENYLALANINE × 1 ZN ZINC ION × 4	ELECTRON MICROSCOPY cryo-EM buffer pH 8 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.00 Å
7ABZ Structure of pre-accomodated trans-translation complex on E. coli stalled ribosome. Deposited 2020-09-09	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 58-meric	Chain L 1–144（144 aa）	Not recorded	MG MAGNESIUM ION × 192 KIR KIRROMYCIN × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ZN ZINC ION × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE；blot for 3.5 seconds before plunging	Resolution 3.21 Å
7AC7 Structure of accomodated trans-translation complex on E. Coli stalled ribosome. Deposited 2020-09-10	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 58-meric	Chain L 1–144（144 aa）	Not recorded	MG MAGNESIUM ION × 441 ZN ZINC ION × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE；blot for 3.5 seconds before plunging	Resolution 3.08 Å
7ACJ Structure of translocated trans-translation complex on E. coli stalled ribosome. Deposited 2020-09-11	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 55-meric	Chain L 1–144（144 aa）	Not recorded	MG MAGNESIUM ION × 308 ZN ZINC ION × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE；blot for 3.5 seconds before plunging	Resolution 3.20 Å
7ACR Structure of post-translocated trans-translation complex on E. coli stalled ribosome. Deposited 2020-09-11	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 55-meric	Chain L 1–144（144 aa）	Not recorded	MG MAGNESIUM ION × 212 ZN ZINC ION × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE；blot for 3.5 seconds before plunging	Resolution 3.44 Å
7B5K E. coli 70S containing suppressor tRNA in the A-site stabilized by a Negamycin analogue and P-site tRNA-nascent chain. Deposited 2020-12-03	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 57-meric	Chain L 1–144（144 aa）	Not recorded	MG MAGNESIUM ION × 359 ERY ERYTHROMYCIN A × 1 SY5 2-[[[(3~{R},5~{R})-3-azanyl-6-(3-azanylpropylamino)-5-oxidanyl-hexanoyl]amino]-methyl-amino]ethanoic acid × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE-PROPANE	Resolution 2.90 Å
7BL2 pre-50S-ObgE particle state 1 Deposited 2021-01-18	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 29 PDB declaration: 31-meric	Chain L 1–144（144 aa）	Not recorded	MG MAGNESIUM ION × 1 ZN ZINC ION × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.70 Å
7BL3 pre-50S-ObgE particle state 2 Deposited 2021-01-18	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 29 PDB declaration: 31-meric	Chain L 1–144（144 aa）	Not recorded	ZN ZINC ION × 1 MG MAGNESIUM ION × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.50 Å
7BL4 in vitro reconstituted 50S-ObgE-GMPPNP-RsfS particle Deposited 2021-01-18	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 31 PDB declaration: 33-meric	Chain L 1–144（144 aa）	Not recorded	ZN ZINC ION × 2 MG MAGNESIUM ION × 6 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.40 Å
7BL5 pre-50S-ObgE particle Deposited 2021-01-18	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 33 PDB declaration: 35-meric	Chain L 1–144（144 aa）	Not recorded	GDP GUANOSINE-5'-DIPHOSPHATE × 1 MG MAGNESIUM ION × 2 ZN ZINC ION × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.30 Å
7BL6 50S-ObgE-GMPPNP particle Deposited 2021-01-18	Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 30 PDB declaration: 32-meric	Chain L 1–144（144 aa）	Not recorded	ZN ZINC ION × 2 MG MAGNESIUM ION × 2 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 4.00 Å
7BV8 Mature 50S ribosomal subunit from RrmJ knock out E.coli strain Deposited 2020-04-09	Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 30 PDB declaration: 32-meric	Chain M 1–144（144 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5；Solutions were made fresh form concentrated to avoid microbial contamination. cryo-EM vitrification conditions Cryogen ETHANE；blot for 2 seconds before plunging	Resolution 3.14 Å
7D6Z Molecular model of the cryo-EM structure of 70S ribosome in complex with peptide deformylase and trigger factor Deposited 2020-10-02	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 58-meric	Chain L 1–144（144 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.40 Å
7D80 Molecular model of the cryo-EM structure of 70S ribosome in complex with peptide deformylase, trigger factor, and methionine aminopeptidase Deposited 2020-10-06	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 57-meric	Chain k 1–144（144 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 4.10 Å
7JSS ArfB Rescue of a 70S Ribosome stalled on truncated mRNA with a partial A-site codon (+2-II) Deposited 2020-08-15	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 54-meric	Chain l 2–144（143 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.70 Å
7JSW ArfB Rescue of a 70S Ribosome stalled on truncated mRNA with a partial A-site codon (+2-III) Deposited 2020-08-16	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 54-meric	Chain l 2–144（143 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.80 Å
7JSZ ArfB Rescue of a 70S Ribosome stalled on truncated mRNA with a partial A-site codon (+2-IV) Deposited 2020-08-16	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 57-meric	Chain l 2–144（143 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.70 Å
7JT1 70S ribosome stalled on long mRNA with ArfB-1 and ArfB-2 bound (+9-III) Deposited 2020-08-16	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric	Chain l 2–144（143 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.30 Å
7JT2 70S ribosome stalled on long mRNA with ArfB bound in the A site Deposited 2020-08-16	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric	Chain l 2–144（143 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.50 Å
7JT3 Rotated 70S ribosome stalled on long mRNA with ArfB-1 and ArfB-2 bound in the A site (+9-IV) Deposited 2020-08-17	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 53 PDB declaration: 58-meric	Chain l 2–144（143 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.70 Å
7K50 Pre-translocation non-frameshifting(CCA-A) complex (Structure I) Deposited 2020-09-16	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 59-meric	Chain l 2–144（143 aa）	Not recorded	FME N-FORMYLMETHIONINE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE；Blotting force 9, blotted for 4 seconds	Resolution 3.40 Å
7K51 Mid-translocated non-frameshifting(CCA-A) complex with EF-G and GDPCP (Structure II) Deposited 2020-09-16	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 53 PDB declaration: 59-meric	Chain l 2–144（143 aa）	Not recorded	FME N-FORMYLMETHIONINE × 1 PRO PROLINE × 1 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE；Blotting force 9, blotted for 4 seconds	Resolution 3.50 Å
7K52 Near post-translocated non-frameshifting(CCA-A) complex with EF-G and GDPCP (Structure III) Deposited 2020-09-16	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 53 PDB declaration: 59-meric	Chain l 2–144（143 aa）	Not recorded	FME N-FORMYLMETHIONINE × 1 PRO PROLINE × 1 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE；blotting force 9, blotted for 4 seconds	Resolution 3.40 Å
7K53 Pre-translocation +1-frameshifting(CCC-A) complex (Structure I-FS) Deposited 2020-09-16	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 59-meric	Chain l 2–144（143 aa）	Not recorded	FME N-FORMYLMETHIONINE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE；Blotting force 9, blotted for 4 seconds	Resolution 3.20 Å
7K54 Mid-translocated +1-frameshifting(CCC-A) complex with EF-G and GDPCP (Structure II-FS) Deposited 2020-09-16	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 53 PDB declaration: 59-meric	Chain l 2–144（143 aa）	Not recorded	FME N-FORMYLMETHIONINE × 1 PRO PROLINE × 1 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE；Blotting force 9, blotted for 4 seconds	Resolution 3.20 Å
7K55 Near post-translocated +1-frameshifting(CCC-A) complex with EF-G and GDPCP (Structure III-FS) Deposited 2020-09-16	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 53 PDB declaration: 59-meric	Chain l 2–144（143 aa）	Not recorded	FME N-FORMYLMETHIONINE × 1 PRO PROLINE × 1 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE；blotting force 9, blotted for 4 seconds	Resolution 3.30 Å
7LV0 Pre-translocation rotated ribosome +1-frameshifting(CCC-A) complex (Structure Irot-FS) Deposited 2021-02-23	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 58-meric	Chain l 2–144（143 aa）	Not recorded	FME N-FORMYLMETHIONINE × 1 PRO PROLINE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE；Blotting force 9, blotted for 4 seconds	Resolution 3.20 Å
7N1P Elongating 70S ribosome complex in a classical pre-translocation (PRE-C) conformation Deposited 2021-05-28	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 58-meric	Chain LO 1–144（144 aa）	Not recorded	PUT 1,4-DIAMINOBUTANE × 18 MG MAGNESIUM ION × 405 ZN ZINC ION × 3 ATP ADENOSINE-5'-TRIPHOSPHATE × 3 SPD SPERMIDINE × 5	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.33 Å
7N2C Elongating 70S ribosome complex in a fusidic acid-stalled intermediate state of translocation bound to EF-G(GDP) (INT2) Deposited 2021-05-28	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 55 PDB declaration: 61-meric	Chain LO 1–144（144 aa）	Not recorded	PUT 1,4-DIAMINOBUTANE × 21 MG MAGNESIUM ION × 197 ZN ZINC ION × 3 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 SPD SPERMIDINE × 3 FUA FUSIDIC ACID × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.72 Å
7N2U Elongating 70S ribosome complex in a hybrid-H1 pre-translocation (PRE-H1) conformation Deposited 2021-05-29	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric	Chain LO 1–144（144 aa）	Not recorded	PUT 1,4-DIAMINOBUTANE × 16 MG MAGNESIUM ION × 368 ZN ZINC ION × 3 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 SPD SPERMIDINE × 5	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.53 Å
7N2V Elongating 70S ribosome complex in a spectinomycin-stalled intermediate state of translocation bound to EF-G in an active, GTP conformation (INT1) Deposited 2021-05-29	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 53 PDB declaration: 59-meric	Chain LO 1–144（144 aa）	Not recorded	SCM SPECTINOMYCIN × 3 PUT 1,4-DIAMINOBUTANE × 17 MG MAGNESIUM ION × 344 ZN ZINC ION × 3 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 SPD SPERMIDINE × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.54 Å
7N30 Elongating 70S ribosome complex in a hybrid-H2* pre-translocation (PRE-H2*) conformation Deposited 2021-05-30	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric	Chain LO 1–144（144 aa）	Not recorded	PUT 1,4-DIAMINOBUTANE × 11 MG MAGNESIUM ION × 296 ZN ZINC ION × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 SPD SPERMIDINE × 4	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.66 Å
7N31 Elongating 70S ribosome complex in a post-translocation (POST) conformation Deposited 2021-05-31	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric	Chain LO 1–144（144 aa）	Not recorded	MG MAGNESIUM ION × 209 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 PUT 1,4-DIAMINOBUTANE × 9 SPD SPERMIDINE × 4 ZN ZINC ION × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.69 Å
7NBU Structure of the HigB1 toxin mutant K95A from Mycobacterium tuberculosis (Rv1955) and its target, the cspA mRNA, on the E. coli Ribosome. Deposited 2021-01-27	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Insufficient information Heteromer；Protein × 50 PDB declaration: 56-meric	Chain k 1–144（144 aa）	Not recorded	MG MAGNESIUM ION × 313 FME N-FORMYLMETHIONINE × 1 ZN ZINC ION × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE；blot for 2s before plunging	Resolution 3.11 Å
7NSO Structure of ErmDL-Erythromycin-stalled 70S E. coli ribosomal complex with P-tRNA Deposited 2021-03-08	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric	Chain L 1–144（144 aa）	Not recorded	ERY ERYTHROMYCIN A × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE-PROPANE	Resolution 2.90 Å
7NSP Structure of ErmDL-Erythromycin-stalled 70S E. coli ribosomal complex with A and P-tRNA Deposited 2021-03-08	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric	Chain L 1–144（144 aa）	Not recorded	ERY ERYTHROMYCIN A × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE-PROPANE	Resolution 3.50 Å
7NSQ Structure of ErmDL-Telithromycin-stalled 70S E. coli ribosomal complex with A and P-tRNA Deposited 2021-03-08	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric	Chain L 1–144（144 aa）	Not recorded	TEL TELITHROMYCIN × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE-PROPANE	Resolution 3.10 Å
7NWT Initiated 70S ribosome in complex with 2A protein from encephalomyocarditis virus (EMCV) Deposited 2021-03-17	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 54 PDB declaration: 59-meric	Chain L 1–144（144 aa）	Not recorded	MG MAGNESIUM ION × 437 FME N-FORMYLMETHIONINE × 1 ZN ZINC ION × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5；Initiated 70S ribosomes in 50 mM Tris-HCl pH 7.5, 70 mM NH4Cl, 30 mM KCl, 7 mM MgCl2 were diluted tenfold into 20 mM HEPES pH 7.5, 100 mM potassium acetate, 1.5 mM MgCl2, 2.0 mM DTT. 2A protein was dialysed (3K MWCO, 277K, 16 h) into the same buffer. Crosslinking reactions of 50 microliters comprising 75 nM ribosomes, 3.0 micromolar 2A and 2.0 mM bis(sulfosuccinimidyl)suberate (BS3) were performed on ice (30 min) immediately prior to grid preparation. cryo-EM vitrification conditions Cryogen ETHANE；Quantifoil R 2/2 400-mesh copper supports were coated with an additional ~ 60 angstrom layer of amorphous, evaporated carbon by flotation and thoroughly dried before use. Grids were made hydrophilic by glow-discharge in air for 30 s. Three microliters of crosslinking reaction was applied to grids which were then blotted for 4.5 s and vitrified by plunging into liquid ethane using a Vitrobot MK IV (FEI) at 277K, 100% relative humidity.	Resolution 2.66 Å
7NWW CspA-27 cotranslational folding intermediate 1 Deposited 2021-03-17	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric	Chain K 1–144（144 aa）	Not recorded	MG MAGNESIUM ION × 413 ZN ZINC ION × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5；50 mM Tris-HCl, 70 mM NH4Cl, 30 mM KCl, 3.5 mM MgCl2, 8 mM putrescine, 0.5 mM spermidine, 1 mM DTT, 1 mM GTP, pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.05 Å
7O19 Cryo-EM structure of an Escherichia coli TnaC-ribosome complex stalled in response to L-tryptophan Deposited 2021-03-29	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric	Chain BL 1–144（144 aa）	Not recorded	MG MAGNESIUM ION × 170 ZN ZINC ION × 3 TRP TRYPTOPHAN × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5；All buffers used during the preparation contained 2 mM L-tryptophan. cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.90 Å
7O1A Cryo-EM structure of an Escherichia coli TnaC(R23F)-ribosome complex stalled in response to L-tryptophan Deposited 2021-03-29	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric	Chain BL 1–144（144 aa）	Not recorded	MG MAGNESIUM ION × 326 K POTASSIUM ION × 147 ZN ZINC ION × 3 TRP TRYPTOPHAN × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5；All buffers used during the preparation contained 2 mM L-tryptophan. cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.40 Å
7O1C Cryo-EM structure of an Escherichia coli TnaC(R23F)-ribosome-RF2 complex stalled in response to L-tryptophan Deposited 2021-03-29	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 56-meric	Chain BL 1–144（144 aa）	Not recorded	MG MAGNESIUM ION × 339 K POTASSIUM ION × 147 ZN ZINC ION × 3 TRP TRYPTOPHAN × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5；All buffers used during the preparation contained 2 mM L-tryptophan. cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.60 Å
7OIF CspA-27 cotranslational folding intermediate 2 Deposited 2021-05-11	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric	Chain K 1–144（144 aa）	Not recorded	MG MAGNESIUM ION × 416 ZN ZINC ION × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5；50 mM Tris-HCl, 70 mM NH4Cl, 30 mM KCl, 3.5 mM MgCl2, 8 mM putrescine, 0.5 mM spermidine, 1 mM DTT, 1 mM GTP, pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.00 Å
7OIG CspA-27 cotranslational folding intermediate 3 Deposited 2021-05-11	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric	Chain K 1–144（144 aa）	Not recorded	MG MAGNESIUM ION × 400 ZN ZINC ION × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5；50 mM Tris-HCl, 70 mM NH4Cl, 30 mM KCl, 3.5 mM MgCl2, 8 mM putrescine, 0.5 mM spermidine, 1 mM DTT, 1 mM GTP, pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.20 Å
7OII CspA-70 cotranslational folding intermediate 2 Deposited 2021-05-11	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric	Chain K 1–144（144 aa）	Not recorded	MG MAGNESIUM ION × 422 ZN ZINC ION × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5；50 mM Tris-HCl, 70 mM NH4Cl, 30 mM KCl, 3.5 mM MgCl2, 8 mM putrescine, 0.5 mM spermidine, 1 mM DTT, 1 mM GTP, pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.00 Å
7OIZ Cryo-EM structure of 70S ribosome stalled with TnaC peptide Deposited 2021-05-13	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric	Chain k 1–144（144 aa）	Not recorded	PAR PAROMOMYCIN × 1 MG MAGNESIUM ION × 308 TRP TRYPTOPHAN × 1 ZN ZINC ION × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.90 Å
7OJ0 Cryo-EM structure of 70S ribosome stalled with TnaC peptide and RF2 Deposited 2021-05-13	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 54 PDB declaration: 59-meric	Chain k 1–144（144 aa）	Not recorded	MG MAGNESIUM ION × 308 TRP TRYPTOPHAN × 1 ZN ZINC ION × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.50 Å
7OT5 CspA-70 cotranslational folding intermediate 1 Deposited 2021-06-09	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric	Chain K 1–144（144 aa）	Not recorded	MG MAGNESIUM ION × 394 ZN ZINC ION × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5；50 mM Tris-HCl, 70 mM NH4Cl, 30 mM KCl, 3.5 mM MgCl2, 8 mM putrescine, 0.5 mM spermidine, 1 mM DTT, 1 mM GTP, pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.90 Å
7P3K Cryo-EM structure of 70S ribosome stalled with TnaC peptide (control) Deposited 2021-07-08	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 54-meric	Chain k 1–144（144 aa）	Not recorded	MG MAGNESIUM ION × 308 CLM CHLORAMPHENICOL × 1 ZN ZINC ION × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.90 Å
7PJS Structure of the 70S ribosome with tRNAs in the classical pre-translocation state and apramycin (C) Deposited 2021-08-24	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 58-meric	Chain L 1–144（144 aa）	Not recorded	MG MAGNESIUM ION × 363 ZN ZINC ION × 2 NA SODIUM ION × 2 AM2 APRAMYCIN × 4	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5；50 mM HEPES, 70 mM NH4Cl, 30 mM KCl, 3.5 mM MgCl2, 0.6 mM spermine, 0.4 mM spermidine cryo-EM vitrification conditions Cryogen ETHANE；Manual blotting & plunge-freezing	Resolution 2.35 Å
7PJT Structure of the 70S ribosome with tRNAs in hybrid state 1 (H1) Deposited 2021-08-24	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 58-meric	Chain L 1–144（144 aa）	Not recorded	ZN ZINC ION × 2 AM2 APRAMYCIN × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE；Manual blotting & plunge-freezing	Resolution 6.00 Å
7PJV Structure of the 70S-EF-G-GDP-Pi ribosome complex with tRNAs in hybrid state 1 (H1-EF-G-GDP-Pi) Deposited 2021-08-24	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 53 PDB declaration: 59-meric	Chain L 1–144（144 aa）	Not recorded	MG MAGNESIUM ION × 369 ZN ZINC ION × 2 NA SODIUM ION × 2 AM2 APRAMYCIN × 3 GDP GUANOSINE-5'-DIPHOSPHATE × 1 PO4 PHOSPHATE ION × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5；50 mM HEPES, 70 mM NH4Cl, 30 mM KCl, 3.5 mM MgCl2, 0.6 mM spermine, 0.4 mM spermidine cryo-EM vitrification conditions Cryogen ETHANE；Manual blotting & plunge-freezing	Resolution 3.10 Å
7PJW Structure of the 70S-EF-G-GDP-Pi ribosome complex with tRNAs in hybrid state 2 (H2-EF-G-GDP-Pi) Deposited 2021-08-24	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 53 PDB declaration: 59-meric	Chain L 1–144（144 aa）	Not recorded	AM2 APRAMYCIN × 1 PO4 PHOSPHATE ION × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5；50 mM HEPES, 70 mM NH4Cl, 30 mM KCl, 3.5 mM MgCl2, 0.6 mM spermine, 0.4 mM spermidine cryo-EM vitrification conditions Cryogen ETHANE；Manual blotting & plunge-freezing	Resolution 4.00 Å
7PJX Structure of the 70S-EF-G-GDP ribosome complex with tRNAs in hybrid state 1 (H1-EF-G-GDP) Deposited 2021-08-24	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 53 PDB declaration: 59-meric	Chain L 1–144（144 aa）	Not recorded	AM2 APRAMYCIN × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5；50 mM HEPES, 70 mM NH4Cl, 30 mM KCl, 3.5 mM MgCl2, 0.6 mM spermine, 0.4 mM spermidine cryo-EM vitrification conditions Cryogen ETHANE；Manual blotting & plunge-freezing	Resolution 6.50 Å
7PJY Structure of the 70S-EF-G-GDP ribosome complex with tRNAs in chimeric state 1 (CHI1-EF-G-GDP) Deposited 2021-08-24	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 53 PDB declaration: 59-meric	Chain L 1–144（144 aa）	Not recorded	MG MAGNESIUM ION × 318 ZN ZINC ION × 2 NA SODIUM ION × 1 AM2 APRAMYCIN × 4 GDP GUANOSINE-5'-DIPHOSPHATE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5；50 mM HEPES, 70 mM NH4Cl, 30 mM KCl, 3.5 mM MgCl2, 0.6 mM spermine, 0.4 mM spermidine cryo-EM vitrification conditions Cryogen ETHANE；Manual blotting & plunge-freezing	Resolution 3.10 Å
7PJZ Structure of the 70S-EF-G-GDP ribosome complex with tRNAs in chimeric state 2 (CHI2-EF-G-GDP) Deposited 2021-08-24	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 53 PDB declaration: 59-meric	Chain L 1–144（144 aa）	Not recorded	ZN ZINC ION × 1 AM2 APRAMYCIN × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5；50 mM HEPES, 70 mM NH4Cl, 30 mM KCl, 3.5 mM MgCl2, 0.6 mM spermine, 0.4 mM spermidine cryo-EM vitrification conditions Cryogen ETHANE；Manual blotting & plunge-freezing	Resolution 6.00 Å
7Q4K Erythromycin-stalled Escherichia coli 70S ribosome with streptococcal MsrDL nascent chain Deposited 2021-10-31	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric	Chain BL 1–144（144 aa）	Not recorded	MG MAGNESIUM ION × 172 ERY ERYTHROMYCIN A × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.00 Å
7QG8 Structure of the collided E. coli disome - VemP-stalled 70S ribosome Deposited 2021-12-07	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 57-meric	Chain Y 1–144（144 aa）	Not recorded	MG MAGNESIUM ION × 2 K POTASSIUM ION × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.97 Å
7QGH Structure of the E. coli disome - collided 70S ribosome Deposited 2021-12-08	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 58-meric	Chain Y 1–144（144 aa）	Not recorded	MG MAGNESIUM ION × 2 K POTASSIUM ION × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 4.48 Å
7QGN Structure of the SmrB-bound E. coli disome - stalled 70S ribosome Deposited 2021-12-09	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 53 PDB declaration: 58-meric	Chain Y 1–144（144 aa）	Not recorded	MG MAGNESIUM ION × 2 K POTASSIUM ION × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.37 Å
7QGR Structure of the SmrB-bound E. coli disome - collided 70S ribosome Deposited 2021-12-09	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 53 PDB declaration: 59-meric	Chain Y 1–144（144 aa）	Not recorded	MG MAGNESIUM ION × 2 K POTASSIUM ION × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 5.70 Å
7QQ3 Cryo-EM structure of the E.coli 50S ribosomal subunit in complex with the antibiotic Myxovalargin A. Deposited 2022-01-06	Different construct Different oligomeric state Different ligand/ion Different experimental conditions	Assembly 1 Protein–RNA Heteromer；Protein × 27 PDB declaration: 29-meric	Chain T 1–143（143 aa）	Not recorded	MG MAGNESIUM ION × 145 ZN ZINC ION × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5；Solutions were made fresh, pH was adjusted with potassium hydroxide at 4 degrees celcius and sterile filtered. cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；2 seconds blotting before plunging.	Resolution 2.10 Å
7SS9 Late translocation intermediate with EF-G partially dissociated (Structure V) Deposited 2021-11-10	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 57-meric	Chain l 2–144（143 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.90 Å
7SSD Mid translocation intermediate with EF-G bound with GDP (Structure IV) Deposited 2021-11-10	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 58-meric	Chain l 2–144（143 aa）	Not recorded	GDP GUANOSINE-5'-DIPHOSPHATE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.30 Å
7SSL Pre translocation intermediate with EF-G bound to GDP and Pi (Structure III) Deposited 2021-11-11	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 58-meric	Chain l 2–144（143 aa）	Not recorded	GDP GUANOSINE-5'-DIPHOSPHATE × 1 PO4 PHOSPHATE ION × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.80 Å
7SSN Pre translocation 70S ribosome with A/P* and P/E tRNA (Structure II-B) Deposited 2021-11-11	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 54-meric	Chain l 2–144（143 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.20 Å
7SSO Pre translocation 70S ribosome with A/A and P/E tRNA (Structure II-A) Deposited 2021-11-11	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 58-meric	Chain l 2–144（143 aa）	Not recorded	FME N-FORMYLMETHIONINE × 1 PRO PROLINE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.20 Å
7SSW Late translocation intermediate with EF-G dissociated (Structure VI) Deposited 2021-11-11	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric	Chain l 2–144（143 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.80 Å
7ST2 Post translocation, non-rotated 70S ribosome with EF-G dissociated (Structure VII) Deposited 2021-11-11	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 58-meric	Chain l 2–144（143 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.90 Å
7ST6 Pre translocation, non-rotated 70S ribosome (Structure I) Deposited 2021-11-12	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 57-meric	Chain l 2–144（143 aa）	Not recorded	PRO PROLINE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.00 Å
7ST7 Pre translocation intermediate stalled with viomycin and bound with EF-G in a GDP and Pi state (Structure III-vio) Deposited 2021-11-12	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 53 PDB declaration: 59-meric	Chain l 2–144（143 aa）	Not recorded	GDP GUANOSINE-5'-DIPHOSPHATE × 1 PO4 PHOSPHATE ION × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.20 Å
7Y7C Structure of the Bacterial Ribosome with human tRNA Asp(G34) and mRNA(GAU) Deposited 2022-06-22	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric	Chain k 1–144（144 aa）	Not recorded	MG MAGNESIUM ION × 283	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6；The Buffer pH was adjusted to 7.6 using KOH. cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.51 Å
7Y7D Structure of the Bacterial Ribosome with human tRNA Asp(Q34) and mRNA(GAU) Deposited 2022-06-22	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric	Chain k 1–144（144 aa）	Not recorded	MG MAGNESIUM ION × 258	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6；The Buffer pH was adjusted to 7.6 using KOH. cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.58 Å
7Y7E Structure of the Bacterial Ribosome with human tRNA Asp(ManQ34) and mRNA(GAU) Deposited 2022-06-22	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric	Chain k 1–144（144 aa）	Not recorded	MG MAGNESIUM ION × 248 MAN alpha-D-mannopyranose × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6；The Buffer pH was adjusted to 7.6 using KOH. cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.41 Å
7Y7F Structure of the Bacterial Ribosome with human tRNA Asp(ManQ34) and mRNA(GAC) Deposited 2022-06-22	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric	Chain k 1–144（144 aa）	Not recorded	MG MAGNESIUM ION × 259 MAN alpha-D-mannopyranose × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6；The Buffer pH was adjusted to 7.6 using KOH. cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.43 Å
7Y7G Structure of the Bacterial Ribosome with human tRNA Tyr(GalQ34) and mRNA(UAU) Deposited 2022-06-22	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric	Chain k 1–144（144 aa）	Not recorded	MG MAGNESIUM ION × 256 GAL beta-D-galactopyranose × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6；The Buffer pH was adjusted to 7.6 using KOH. cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.34 Å
7Y7H Structure of the Bacterial Ribosome with human tRNA Tyr(GalQ34) and mRNA(UAC) Deposited 2022-06-22	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric	Chain k 1–144（144 aa）	Not recorded	MG MAGNESIUM ION × 263 GAL beta-D-galactopyranose × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6；The Buffer pH was adjusted to 7.6 using KOH. cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.51 Å
7YLA Cryo-EM structure of 50S-HflX complex Deposited 2022-07-25	Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 30 PDB declaration: 32-meric	Chain T 1–144（144 aa）	Not recorded	GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 3 NA SODIUM ION × 1 ZN ZINC ION × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.52 Å
7Z20 70S E. coli ribosome with an extended uL23 loop from Candidatus marinimicrobia and a stalled filamin domain 5 nascent chain Deposited 2022-02-25	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 29 PDB declaration: 32-meric	Chain l 1–144（144 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.29 Å
7ZOD 70S E. coli ribosome with an extended uL23 loop from Candidatus marinimicrobia Deposited 2022-04-25	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 28 PDB declaration: 31-meric	Chain l 1–144（144 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.56 Å
7ZP8 70S E. coli ribosome with a stalled filamin domain 5 nascent chain Deposited 2022-04-26	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 29 PDB declaration: 32-meric	Chain l 1–144（144 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.20 Å
7ZQ5 70S E. coli ribosome with truncated uL23 and uL24 loops Deposited 2022-04-29	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 28 PDB declaration: 30-meric	Chain l 1–144（144 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.70 Å
7ZQ6 70S E. coli ribosome with truncated uL23 and uL24 loops and a stalled filamin domain 5 nascent chain Deposited 2022-04-29	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 29 PDB declaration: 32-meric	Chain l 1–144（144 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.75 Å
7ZTA Structure of an Escherichia coli 70S ribosome stalled by Tetracenomycin X during translation of an MAAAPQK(C) peptide Deposited 2022-05-09	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 57-meric	Chain L151 1–144（144 aa）	Not recorded	MG MAGNESIUM ION × 289 K POTASSIUM ION × 115 ZN ZINC ION × 3 OCW Tetracenomycin X × 1 CYS CYSTEINE × 1 GLN GLUTAMINE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE；Waiting 30 sec Blotting 2.5 sec Blot force 5	Resolution 2.70 Å
8A3L Structural insights into the binding of bS1 to the ribosome Deposited 2022-06-08	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric	Chain k 1–144（144 aa）	Not recorded	MG MAGNESIUM ION × 313 ZN ZINC ION × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.42 Å
8AKN Cryo-EM structure of the proline-rich antimicrobial peptide drosocin bound to the terminating ribosome Deposited 2022-07-30	Different oligomeric state Different ligand/ion Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 57-meric	Chain k 1–144（144 aa）	Not recorded	ZN ZINC ION × 2 A2G 2-acetamido-2-deoxy-alpha-D-galactopyranose × 1 MG MAGNESIUM ION × 305 SPM SPERMINE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE-PROPANE	Resolution 2.30 Å
8AM9 Cryo-EM structure of the proline-rich antimicrobial peptide drosocin bound to the elongating ribosome Deposited 2022-08-03	Different oligomeric state Different ligand/ion Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric	Chain k 1–144（144 aa）	Not recorded	ZN ZINC ION × 2 MG MAGNESIUM ION × 293 SPM SPERMINE × 1 A2G 2-acetamido-2-deoxy-alpha-D-galactopyranose × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE-PROPANE	Resolution 2.80 Å
8AP4 Structure of Escherischia coli heat shock protein Hsp15 in complex with ribosomal 50S subunits bearing peptidyl-tRNA Deposited 2022-08-09	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 30 PDB declaration: 33-meric	Chain k 1–144（144 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.46 cryo-EM vitrification conditions Cryogen ETHANE-PROPANE	Resolution 3.00 Å
8AYE E. coli 70S ribosome bound to thermorubin and fMet-tRNA Deposited 2022-09-02	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric	Chain k 1–144（144 aa）	Not recorded	ZN ZINC ION × 2 MG MAGNESIUM ION × 308 T8B Thermorubin × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 1.96 Å
8B0X Translating 70S ribosome in the unrotated state (P and E, tRNAs) Deposited 2022-09-08	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 53-meric	Chain k 1–144（144 aa）	Not recorded	ZN ZINC ION × 1 K POTASSIUM ION × 168 MG MAGNESIUM ION × 361	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 1.55 Å
8B7Y Cryo-EM structure of the E.coli 70S ribosome in complex with the antibiotic Myxovalargin B. Deposited 2022-10-03	Different oligomeric state Different ligand/ion Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 47 PDB declaration: 52-meric	Chain T 1–144（144 aa）	Not recorded	MG MAGNESIUM ION × 237 ZN ZINC ION × 1 FME N-FORMYLMETHIONINE × 1 SPD SPERMIDINE × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE-PROPANE	Resolution 3.00 Å
8BF7 Elongating E. coli 70S ribosome containing deacylated tRNA(iMet) in the P-site and AAA mRNA codon with cognate dipeptidyl-tRNA(Lys) in the A-site Deposited 2022-10-24	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric	Chain I 1–144（144 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5；1x TAKM7 buffer cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.33 Å
8BGE Elongating E. coli 70S ribosome containing acylated tRNA(iMet) in the P-site and AAm6A mRNA codon in the A-site after uncompleted di-peptide formation Deposited 2022-10-27	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 53-meric	Chain I 1–144（144 aa）	Not recorded	FME N-FORMYLMETHIONINE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5；1x TAKM7 buffer cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.11 Å
8BGH Elongating E. coli 70S ribosome containing acylated tRNA(iMet) in the P-site and AAA mRNA codon in the A-site after uncompleted di-peptide formation Deposited 2022-10-27	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 54-meric	Chain I 1–144（144 aa）	Not recorded	FME N-FORMYLMETHIONINE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5；1x TAKM7 buffer cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.88 Å
8BH4 Elongating E. coli 70S ribosome containing deacylated tRNA(iMet) in the P-site and AAm6A mRNA codon with cognate dipeptidyl-tRNA(Lys) in the A-site Deposited 2022-10-29	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric	Chain I 1–144（144 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5；1x TAKM7 buffer cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.62 Å
8BHJ Elongating E. coli 70S ribosome containing deacylated tRNA(iMet) in the P-site and Am6AA mRNA codon with cognate dipeptidyl-tRNA(Lys) in the A-site Deposited 2022-10-31	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric	Chain I 1–144（144 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5；1x TAKM7 buffer cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.81 Å
8BHL Elongating E. coli 70S ribosome containing acylated tRNA(iMet) in the P-site and Am6AA mRNA codon in the A-site after uncompleted di-peptide formation Deposited 2022-10-31	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 53-meric	Chain I 1–144（144 aa）	Not recorded	FME N-FORMYLMETHIONINE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5；1x TAKM7 buffer cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.21 Å
8BHN Elongating E. coli 70S ribosome containing deacylated tRNA(iMet) in the P-site and m6AAA mRNA codon with cognate dipeptidyl-tRNA(Lys) in the A-site Deposited 2022-10-31	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric	Chain I 1–144（144 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5；1x TAKM7 buffer cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.85 Å
8BHP Elongating E. coli 70S ribosome containing acylated tRNA(iMet) in the P-site and m6AAA mRNA codon in the A-site after uncompleted di-peptide formation Deposited 2022-10-31	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 54-meric	Chain I 1–144（144 aa）	Not recorded	FME N-FORMYLMETHIONINE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5；1x TAKM7 buffer cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.37 Å
8BIL Initiation complex of the E. coli 70S ribosome with mRNA containing AAA codon in the A-site. Deposited 2022-11-02	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 54-meric	Chain I 1–144（144 aa）	Not recorded	FME N-FORMYLMETHIONINE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5；1x TAKM7 buffer cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.04 Å
8BIM Initiation complex of the E. coli 70S ribosome with mRNA containing AAm6A codon in the A-site Deposited 2022-11-02	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 54-meric	Chain I 1–144（144 aa）	Not recorded	FME N-FORMYLMETHIONINE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5；1x TAKM7 buffer cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.04 Å
8C8X Cryo-EM captures early ribosome assembly in action Deposited 2023-01-21	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 26 PDB declaration: 28-meric	Chain L 1–144（144 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.93 Å
8C8Y Cryo-EM captures early ribosome assembly in action Deposited 2023-01-21	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 23 PDB declaration: 25-meric	Chain L 1–144（144 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.03 Å
8C8Z Cryo-EM captures early ribosome assembly in action Deposited 2023-01-21	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 22 PDB declaration: 24-meric	Chain L 1–144（144 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.12 Å
8C90 Cryo-EM captures early ribosome assembly in action Deposited 2023-01-21	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 20 PDB declaration: 22-meric	Chain L 1–144（144 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.15 Å
8C91 Cryo-EM captures early ribosome assembly in action Deposited 2023-01-21	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 17 PDB declaration: octadecameric	Chain L 1–144（144 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 4.19 Å
8C92 Cryo-EM captures early ribosome assembly in action Deposited 2023-01-21	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 16 PDB declaration: heptadecameric	Chain L 1–144（144 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.79 Å
8C93 Cryo-EM captures early ribosome assembly in action Deposited 2023-01-21	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 16 PDB declaration: heptadecameric	Chain L 1–144（144 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 4.17 Å
8C94 Cryo-EM captures early ribosome assembly in action Deposited 2023-01-21	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 19 PDB declaration: 21-meric	Chain L 1–144（144 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.80 Å
8C95 Cryo-EM captures early ribosome assembly in action Deposited 2023-01-21	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 14 PDB declaration: hexadecameric	Chain L 1–144（144 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 4.92 Å
8C96 Cryo-EM captures early ribosome assembly in action Deposited 2023-01-21	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 15 PDB declaration: hexadecameric	Chain L 1–144（144 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 4.43 Å
8C98 Cryo-EM captures early ribosome assembly in action Deposited 2023-01-21	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 11 PDB declaration: dodecameric	Chain L 1–144（144 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.66 Å
8C99 Cryo-EM captures early ribosome assembly in action Deposited 2023-01-21	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 10 PDB declaration: undecameric	Chain L 1–144（144 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.29 Å
8CAM Evernimicin bound to the 50S subunit Deposited 2023-01-24	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 26 PDB declaration: 28-meric	Chain k 1–144（144 aa）	Not recorded	ZN ZINC ION × 1 ACT ACETATE ION × 1 MG MAGNESIUM ION × 246 K POTASSIUM ION × 72 6O1 Evernimicin × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 1.86 Å
8CEU Retapamulin and Capreomycin bound to the 50S subunit Deposited 2023-02-02	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 36 PDB declaration: 38-meric	Chain k 1–144（144 aa）	Not recorded	ZN ZINC ION × 1 G34 Retapamulin × 1 MG MAGNESIUM ION × 220 K POTASSIUM ION × 81	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 1.83 Å
8CGD Clindamycin bound to the 50S subunit Deposited 2023-02-03	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 28 PDB declaration: 30-meric	Chain k 1–144（144 aa）	Not recorded	ZN ZINC ION × 2 ACT ACETATE ION × 1 CLY CLINDAMYCIN × 1 AM2 APRAMYCIN × 1 MG MAGNESIUM ION × 254 K POTASSIUM ION × 85	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 1.98 Å
8CGK Lincomycin and Avilamycin bound to the 50S subunit Deposited 2023-02-05	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 26 PDB declaration: 28-meric	Chain k 1–144（144 aa）	Not recorded	ZN ZINC ION × 1 ACT ACETATE ION × 1 3QB LINCOMYCIN × 1 6UQ (2R,3S,4R,6S)-4-hydroxy-6-{[(2R,3aR,4R,4'R,5'S,6S,6'R,7aR)-4'-hydroxy-6-{[(2S,3R,4R,5S,6R)-3-hydroxy-2-{[(2R,3S,4S,5S,6S)-4-hydroxy-6-({(2R,3aS,3a'R,6S,6'R,7R,7'R,7aR,7a'R)-7'-hydroxy-7'-[(1S)-1-hydroxyethyl]-6'-methyl-7-[(2-methylpropanoyl)oxy]octahydro-4H-2,4'-spirobi[[1,3]dioxolo[4,5-c]pyran]-6-yl}oxy)-5-methoxy-2-(methoxymethyl)tetrahydro-2H-pyran-3-yl]oxy}-5-methoxy-6-methyltetrahydro-2H-pyran-4-yl]oxy}-4,6',7a-trimethyloctahydro-4H-spiro[1,3-dioxolo[4,5-c]pyran-2,2'-pyran]-5'-yl]oxy}-2-methyltetrahydro-2H-pyran-3-yl 3,5-dichloro-4-hydroxy-2-methoxy-6-methylbenzoate (non-preferred name) × 1 MG MAGNESIUM ION × 260 K POTASSIUM ION × 81	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 1.64 Å
8CGV Tiamulin bound to the 50S subunit Deposited 2023-02-06	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 27 PDB declaration: 29-meric	Chain k 1–144（144 aa）	Not recorded	ZN ZINC ION × 1 P8F Pentacycline × 2 MUL TIAMULIN × 1 MG MAGNESIUM ION × 219 K POTASSIUM ION × 83	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 1.66 Å
8HSP E. coli 70S ribosome complexed with tRNA_Ile2 bearing L34 and t6A37 in classical state Deposited 2022-12-20	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric	Chain k 1–144（144 aa）	Not recorded	MG MAGNESIUM ION × 315 K POTASSIUM ION × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6；The Buffer pH was adjusted to 7.6 using KOH. cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.32 Å
8HTZ E. coli 70S ribosome complexed with H. marismortui tRNA_Ile2 bearing agm2C34 in classical state Deposited 2022-12-22	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric	Chain k 1–144（144 aa）	Not recorded	MG MAGNESIUM ION × 317 K POTASSIUM ION × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6；The Buffer pH was adjusted to 7.6 using KOH. cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.40 Å
8HU1 E. coli 70S ribosome complexed with tRNA_Ile2 bearing L34 and ct6A37 in classical state Deposited 2022-12-22	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric	Chain k 1–144（144 aa）	Not recorded	MG MAGNESIUM ION × 314 K POTASSIUM ION × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6；The Buffer pH was adjusted to 7.6 using KOH. cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.69 Å
8IFB Dibekacin-bound E.coli 70S ribosome in the PURE system Deposited 2023-02-17	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric	Chain k 1–144（144 aa）	Not recorded	MG MAGNESIUM ION × 406 SPD SPERMIDINE × 14 84D Dibekacin × 13 SPM SPERMINE × 1 ZN ZINC ION × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.43 Å
8IFC Arbekacin-bound E.coli 70S ribosome in the PURE system Deposited 2023-02-17	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric	Chain k 1–144（144 aa）	Not recorded	MG MAGNESIUM ION × 460 SPD SPERMIDINE × 16 84G Arbekacin × 10 SPM SPERMINE × 1 ZN ZINC ION × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.90 Å
8J1Z The global structure of pre50S related to DbpA in state3 Deposited 2023-04-13	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 25 PDB declaration: 27-meric	Chain k 1–144（144 aa）	Not recorded	MG MAGNESIUM ION × 210	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5；20mM HEPES-KOH,pH7.5;140mM NH4Cl;10mM Mg2+,1mM DTT cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.60 Å
8P16 E167K RF2 on E. coli 70S release complex with UGG (Structure I) Deposited 2023-05-11	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 56-meric	Chain L 1–144（144 aa）	Not recorded	MG MAGNESIUM ION × 330 SPD SPERMIDINE × 13 K POTASSIUM ION × 128 PUT 1,4-DIAMINOBUTANE × 13 SPM SPERMINE × 1 ZN ZINC ION × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5；HEPES-polymix buffer (pH-7.5) cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.77 Å
8P17 E167K RF2 on E. coli 70S release complex with UGG (Structure II) Deposited 2023-05-11	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 56-meric	Chain L 1–144（144 aa）	Not recorded	MG MAGNESIUM ION × 325 SPD SPERMIDINE × 13 K POTASSIUM ION × 128 PUT 1,4-DIAMINOBUTANE × 13 SPM SPERMINE × 1 ZN ZINC ION × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5；HEPES-polymix buffer (pH-7.5) cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.78 Å
8P18 E167K RF2 on E. coli 70S release complex with UGG (Structure III) Deposited 2023-05-11	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 56-meric	Chain L 1–144（144 aa）	Not recorded	MG MAGNESIUM ION × 321 SPD SPERMIDINE × 13 K POTASSIUM ION × 127 PUT 1,4-DIAMINOBUTANE × 13 SPM SPERMINE × 1 ZN ZINC ION × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5；HEPES-polymix buffer (pH-7.5) cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.77 Å
8PEG Escherichia coli paused disome complex (queueing 70S non-rotated closed PRE state) Deposited 2023-06-13	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 54 PDB declaration: 61-meric	Chain o 1–144（144 aa）	Not recorded	ZN ZINC ION × 2 MG MAGNESIUM ION × 260 ATP ADENOSINE-5'-TRIPHOSPHATE × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6 cryo-EM vitrification conditions Cryogen ETHANE；Withdrawn samples were spotted directly onto freshly glow-discharged holey carbon grids, blotted for 1-2 s, and flash frozen in liquid ethane using a Vitrobot Mark IV plunger (ThermoFisher Scientific) after a wait time of 40 s at 4 degrees Celcius.	Resolution 3.30 Å
8PHJ cA4-bound Cami1 in complex with 70S ribosome Deposited 2023-06-20	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 54 PDB declaration: 61-meric	Chain k 1–144（144 aa）	Not recorded	ZN ZINC ION × 2 MG MAGNESIUM ION × 241	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.67 Å
8PKL Escherichia coli paused disome complex (leading 70S non-rotated closed PRE state) Deposited 2023-06-26	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 53 PDB declaration: 60-meric	Chain o 1–144（144 aa）	Not recorded	ZN ZINC ION × 2 PUT 1,4-DIAMINOBUTANE × 2 SPD SPERMIDINE × 1 MG MAGNESIUM ION × 287 ALA ALANINE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6 cryo-EM vitrification conditions Cryogen ETHANE；Withdrawn samples were spotted directly onto freshly glow-discharged holey carbon grids, blotted for 1-2 s, and flash frozen in liquid ethane using a Vitrobot Mark IV plunger (ThermoFisher Scientific) after a wait time of 40 s at 4 degrees Celcius.	Resolution 3.09 Å
8PVA Structure of bacterial ribosome determined by cryoEM at 100 keV Deposited 2023-07-17	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric	Chain k 1–144（144 aa）	Not recorded	ZN ZINC ION × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 4.50 Å
8QBT E. coli ApdP-stalled ribosomal complex Deposited 2023-08-25	Different oligomeric state Different ligand/ion Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 45 PDB declaration: 52-meric	Chain L 1–144（144 aa）	Not recorded	MG MAGNESIUM ION × 248 K POTASSIUM ION × 127 PRO PROLINE × 1 ZN ZINC ION × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE-PROPANE	Resolution 2.20 Å
8QK7 E167K RF2 on E. coli 70S release complex with UAA Deposited 2023-09-14	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 56-meric	Chain L 1–144（144 aa）	Not recorded	MG MAGNESIUM ION × 332 PUT 1,4-DIAMINOBUTANE × 21 K POTASSIUM ION × 126 SPD SPERMIDINE × 10 SPM SPERMINE × 2 ZN ZINC ION × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5；HEPES-polymix buffer (pH-7.5) cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.77 Å
8QOA Structure of SecM-stalled Escherichia coli 70S ribosome Deposited 2023-09-28	Different oligomeric state Different ligand/ion Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 57-meric	Chain k 1–144（144 aa）	Not recorded	ZN ZINC ION × 2 K POTASSIUM ION × 132 MG MAGNESIUM ION × 288 PRO PROLINE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE-PROPANE	Resolution 2.00 Å
8R3V Escherichia coli paused disome complex (non-rotated disome interface) Deposited 2023-11-10	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 54 PDB declaration: 65-meric	Chain o2 1–144（144 aa）	Not recorded	ZN ZINC ION × 2 PUT 1,4-DIAMINOBUTANE × 2 SPD SPERMIDINE × 1 MG MAGNESIUM ION × 297 ALA ALANINE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6 cryo-EM vitrification conditions Cryogen ETHANE；Withdrawn samples were spotted directly onto freshly glow-discharged holey carbon grids, blotted for 1-2 s, and flash frozen in liquid ethane using a Vitrobot Mark IV plunger (ThermoFisher Scientific) after a wait time of 40 s at 4 degrees Celsius.	Resolution 3.28 Å
8R6C 70S Escherichia coli ribosome with Paenilamicin B2 bound with A- and P-site tRNA. Deposited 2023-11-22	Different oligomeric state Different ligand/ion Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 57-meric	Chain k 1–144（144 aa）	Not recorded	ZN ZINC ION × 2 MG MAGNESIUM ION × 308 K POTASSIUM ION × 3	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE-PROPANE	Resolution 2.20 Å
8R8M 70S Escherichia coli ribosome with Paenilamicin B2 bound with hybrid A/P- and hybrid P/E-tRNA. Deposited 2023-11-29	Different oligomeric state Different ligand/ion Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric	Chain k 1–144（144 aa）	Not recorded	ZN ZINC ION × 2 MG MAGNESIUM ION × 307 K POTASSIUM ION × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE-PROPANE	Resolution 2.40 Å
8RCL Escherichia coli paused disome complex (Non-rotated disome interface class 1) Deposited 2023-12-06	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 54 PDB declaration: 65-meric	Chain o2 1–144（144 aa）	Not recorded	ZN ZINC ION × 2 PUT 1,4-DIAMINOBUTANE × 2 SPD SPERMIDINE × 1 MG MAGNESIUM ION × 296 ALA ALANINE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6 cryo-EM vitrification conditions Cryogen ETHANE；Withdrawn samples were spotted directly onto freshly glow-discharged holey carbon grids, blotted for 1-2 s, and flash frozen in liquid ethane using a Vitrobot Mark IV plunger (ThermoFisher Scientific) after a wait time of 40 s at 4 degrees Celcius.	Resolution 3.49 Å
8RCM Escherichia coli paused disome complex (Non-rotated disome interface class 2) Deposited 2023-12-06	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 54 PDB declaration: 65-meric	Chain o2 1–144（144 aa）	Not recorded	ZN ZINC ION × 2 PUT 1,4-DIAMINOBUTANE × 2 MG MAGNESIUM ION × 296 ALA ALANINE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6 cryo-EM vitrification conditions Cryogen ETHANE；Withdrawn samples were spotted directly onto freshly glow-discharged holey carbon grids, blotted for 1-2 s, and flash frozen in liquid ethane using a Vitrobot Mark IV plunger (ThermoFisher Scientific) after a wait time of 40 s at 4 degrees Celcius.	Resolution 3.59 Å
8RCS Escherichia coli paused disome complex (Rotated disome interface class 1) Deposited 2023-12-07	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 55 PDB declaration: 65-meric	Chain o2 1–144（144 aa）	Not recorded	ZN ZINC ION × 2 PUT 1,4-DIAMINOBUTANE × 2 SPD SPERMIDINE × 1 MG MAGNESIUM ION × 269	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6 cryo-EM vitrification conditions Cryogen ETHANE；Withdrawn samples were spotted directly onto freshly glow-discharged holey carbon grids, blotted for 1-2 s, and flash frozen in liquid ethane using a Vitrobot Mark IV plunger (ThermoFisher Scientific) after a wait time of 40 s at 4 degrees Celcius.	Resolution 4.46 Å
8RCT Escherichia coli paused disome complex (Rotated disome interface class 2) Deposited 2023-12-07	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 55 PDB declaration: 65-meric	Chain o2 1–144（144 aa）	Not recorded	ZN ZINC ION × 2 PUT 1,4-DIAMINOBUTANE × 2 SPD SPERMIDINE × 1 MG MAGNESIUM ION × 268	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6 cryo-EM vitrification conditions Cryogen ETHANE；Withdrawn samples were spotted directly onto freshly glow-discharged holey carbon grids, blotted for 1-2 s, and flash frozen in liquid ethane using a Vitrobot Mark IV plunger (ThermoFisher Scientific) after a wait time of 40 s at 4 degrees Celcius.	Resolution 5.32 Å
8RPY Escherichia coli 50S subunit in complex with the antimicrobial peptide Api137 Deposited 2024-01-17	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 31 PDB declaration: 33-meric	Chain L 2–144（143 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.64 Å
8RPZ Escherichia coli 50S subunit in complex with the antimicrobial peptide Api88 - conformation I Deposited 2024-01-17	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 32 PDB declaration: 34-meric	Chain L 2–144（143 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.44 Å
8RQ0 Escherichia coli 50S subunit in complex with the antimicrobial peptide Api88 - conformation II Deposited 2024-01-17	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 32 PDB declaration: 34-meric	Chain L 2–144（143 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.44 Å
8RQ2 Escherichia coli 50S subunit in complex with the antimicrobial peptide Api88 - conformation III Deposited 2024-01-17	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 32 PDB declaration: 34-meric	Chain L 2–144（143 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.44 Å
8VOS Escherichia coli transcription-translation loosely coupled complex (TTC-LC) containing mRNA with a 60 nt long spacer, NusG, NusA, and fMet-tRNAs in E-site and P-site Deposited 2024-01-15	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Other combination Heteromer；Protein × 60 PDB declaration: 68-meric	Chain u 1–144（144 aa）	Not recorded	MG MAGNESIUM ION × 1 ZN ZINC ION × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 4.60 Å
8W51 Structure of YchF(H114A) on E.coli 50S ribosomal subunit Deposited 2023-08-25	Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 30 PDB declaration: 32-meric	Chain M 1–144（144 aa）	Not recorded	MG MAGNESIUM ION × 104 ZN ZINC ION × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.40 Å
8Y5K E.coli transcription translation coupling complex in TTC-A state 2 containing mRNA with 27-mer spacer, NusG, NusA, fMet-tRNA(iMet), Phe-tRNA(Phe), and viomycin Deposited 2024-01-31	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Other combination Heteromer；Protein × 59 PDB declaration: 67-meric	Chain l 1–144（144 aa）	Not recorded	MG MAGNESIUM ION × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 PO4 PHOSPHATE ION × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 6.90 Å
8Y5L E.coli transcription translation coupling complex in TTC-B state 1 (subclass1) containing mRNA with 30-mer spacer, NusG, NusA, fMet-tRNA(iMet), Phe-tRNA(Phe), and viomycin Deposited 2024-01-31	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Other combination Heteromer；Protein × 58 PDB declaration: 67-meric	Chain l 1–144（144 aa）	Not recorded	MG MAGNESIUM ION × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.70 Å
8Y5M E.coli transcription translation coupling complex in TTC-B state 2 containing mRNA with 27-mer spacer, NusG, NusA, fMet-tRNA(iMet), Phe-tRNA(Phe), and viomycin Deposited 2024-01-31	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Other combination Heteromer；Protein × 60 PDB declaration: 68-meric	Chain l 1–144（144 aa）	Not recorded	MG MAGNESIUM ION × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 PO4 PHOSPHATE ION × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 4.50 Å
8Y5N E.coli transcription translation coupling complex in TTC-A state 3 containing mRNA with 21-mer spacer, NusG, NusA, fMet-tRNA(iMet), Phe-tRNA(Phe), and viomycin Deposited 2024-01-31	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Other combination Heteromer；Protein × 59 PDB declaration: 67-meric	Chain l 1–144（144 aa）	Not recorded	GDP GUANOSINE-5'-DIPHOSPHATE × 1 PO4 PHOSPHATE ION × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 4.50 Å
8Y5O E.coli transcription translation coupling complex in TTC-B state 3 (subclass1) containing mRNA with 30-mer spacer, NusG, NusA, fMet-tRNA(iMet), Phe-tRNA(Phe), and viomycin Deposited 2024-01-31	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Other combination Heteromer；Protein × 60 PDB declaration: 68-meric	Chain l 1–144（144 aa）	Not recorded	MG MAGNESIUM ION × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 PO4 PHOSPHATE ION × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 4.20 Å
8Y5P E.coli transcription translation coupling complex in TTC-B state 4 (subclass 1) containing mRNA with 24-mer spacer, NusG, NusA, fMet-tRNA(iMet), Phe-tRNA(Phe), and viomycin Deposited 2024-01-31	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Other combination Heteromer；Protein × 60 PDB declaration: 67-meric	Chain l 1–144（144 aa）	Not recorded	MG MAGNESIUM ION × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 PO4 PHOSPHATE ION × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 6.50 Å
8Y5Q E.coli transcription translation coupling complex in TTC-B state 4 (subclass 2) containing mRNA with 27-mer spacer, NusG, NusA, fMet-tRNA(iMet), Phe-tRNA(Phe), and GDPCP Deposited 2024-01-31	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Other combination Heteromer；Protein × 59 PDB declaration: 66-meric	Chain l 1–144（144 aa）	Not recorded	MG MAGNESIUM ION × 1 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.90 Å
8Y5R E.coli Transcription translation coupling complex in TTC-B state 5 (subclass 1) containing mRNA with 27-mer spacer, NusG, NusA, fMet-tRNA(iMet), Phe-tRNA(Phe), and fusidic acid Deposited 2024-01-31	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Other combination Heteromer；Protein × 58 PDB declaration: 66-meric	Chain l 1–144（144 aa）	Not recorded	MG MAGNESIUM ION × 1 FUA FUSIDIC ACID × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 4.50 Å
8Y5S E.coli Transcription translation coupling complex in TTC-B state 5 (subclass 2) containing mRNA with 27-mer spacer, NusG, NusA, fMet-tRNA(iMet), Phe-tRNA(Phe), and GDPCP Deposited 2024-01-31	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Other combination Heteromer；Protein × 58 PDB declaration: 66-meric	Chain l 1–144（144 aa）	Not recorded	MG MAGNESIUM ION × 1 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 4.50 Å
8Y5T E.coli Transcription translation coupling complex in TTC-B state 5 (subclass 3) containing mRNA with 27-mer spacer, NusG, NusA, fMet-tRNA(iMet), Phe-tRNA(Phe), and fusidic acid Deposited 2024-01-31	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Other combination Heteromer；Protein × 58 PDB declaration: 66-meric	Chain l 1–144（144 aa）	Not recorded	MG MAGNESIUM ION × 1 FUA FUSIDIC ACID × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 4.40 Å
8YDE E.coli transcription translation coupling complex in TTC-B state 1 (subclass 3) containing mRNA with 39-mer spacer, NusG, NusA, fMet-tRNA(iMet), Phe-tRNA(Phe), and viomycin Deposited 2024-02-20	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Other combination Heteromer；Protein × 58 PDB declaration: 67-meric	Chain l 1–144（144 aa）	Not recorded	MG MAGNESIUM ION × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 5.00 Å
8YDF E.coli transcription translation coupling complex in TTC-B state 1 (subclass 2) containing mRNA with 39-mer spacer, NusG, NusA, fMet-tRNA(iMet), Phe-tRNA(Phe), and viomycin Deposited 2024-02-20	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Other combination Heteromer；Protein × 58 PDB declaration: 67-meric	Chain l 1–144（144 aa）	Not recorded	MG MAGNESIUM ION × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 4.60 Å
8YDG E.coli transcription translation coupling complex in TTC-B state 3 (subclass2) containing mRNA with 39-mer spacer, NusG, NusA, fMet-tRNA(iMet), Phe-tRNA(Phe), and viomycin Deposited 2024-02-20	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Other combination Heteromer；Protein × 60 PDB declaration: 68-meric	Chain l 1–144（144 aa）	Not recorded	MG MAGNESIUM ION × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 PO4 PHOSPHATE ION × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 5.10 Å
8YDH E.coli transcription translation coupling complex in TTC-P state 1 (subclass1) containing mRNA with 39-mer spacer, NusG, NusA, fMet-tRNA(iMet), Phe-tRNA(Phe), and viomycin Deposited 2024-02-20	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Other combination Heteromer；Protein × 58 PDB declaration: 67-meric	Chain l 1–144（144 aa）	Not recorded	MG MAGNESIUM ION × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 4.55 Å
8YDI E.coli transcription translation coupling complex in TTC-P state 1 (subclass 2) containing mRNA with 39-mer spacer, NusG, NusA, fMet-tRNA(iMet), Phe-tRNA(Phe), and viomycin Deposited 2024-02-20	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Other combination Heteromer；Protein × 58 PDB declaration: 67-meric	Chain l 1–144（144 aa）	Not recorded	MG MAGNESIUM ION × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 4.60 Å
8YDJ E.coli transcription translation coupling complex in TTC-P containing mRNA with 39-mer spacer, NusG, NusA, fMet-tRNA(iMet), Phe-tRNA(Phe), and viomycin Deposited 2024-02-20	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Other combination Heteromer；Protein × 60 PDB declaration: 68-meric	Chain l 1–144（144 aa）	Not recorded	MG MAGNESIUM ION × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 PO4 PHOSPHATE ION × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 5.10 Å
8YUO E. coli 70S ribosome complexed with P. putida tRNAIle2 at the A-site and P-site Deposited 2024-03-27	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric	Chain k 1–144（144 aa）	Not recorded	MG MAGNESIUM ION × 309	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6；The Buffer pH was adjusted to 7.6 using KOH. cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.25 Å
8YUP E. coli 70S ribosome complexed with P. putida tRNAIle2 and A4 mRNA Deposited 2024-03-28	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric	Chain k 1–144（144 aa）	Not recorded	MG MAGNESIUM ION × 309	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6；The Buffer pH was adjusted to 7.6 using KOH. cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.39 Å
8YUQ E. coli 70S ribosome complexed with P. putida tRNAIle2 and dA4 mRNA Deposited 2024-03-27	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric	Chain k 1–144（144 aa）	Not recorded	MG MAGNESIUM ION × 309	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6；The Buffer pH was adjusted to 7.6 using KOH. cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.41 Å
8YUR E. coli 70S ribosome complexed with P. putida tRNAIle2 and Am4 mRNA Deposited 2024-03-27	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric	Chain k 1–144（144 aa）	Not recorded	MG MAGNESIUM ION × 309	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6；The Buffer pH was adjusted to 7.6 using KOH. cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.47 Å
8YUS E. coli 70S ribosome complexed with P.putida tRNAIle2 and A(F)4 mRNA Deposited 2024-03-27	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric	Chain k 1–144（144 aa）	Not recorded	MG MAGNESIUM ION × 309	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6；The Buffer pH was adjusted to 7.6 using KOH. cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.43 Å
8ZEJ Structure of E.coli ribosome in complex with an engineered arrest peptide Deposited 2024-05-06	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric	Chain m 1–144（144 aa）	Not recorded	ZN ZINC ION × 1 PRO PROLINE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6；20 mM HEPES (pH 7.6), 140 mM NH4Cl, 25 mM MgCl2, 2 mM DTT cryo-EM vitrification conditions Cryogen NITROGEN	Resolution 2.69 Å
8ZFF Structure of the Bacterial Ribosome without hypoxia-induced rRNA modifications Deposited 2024-05-07	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 54-meric	Chain k 1–144（144 aa）	Not recorded	MG MAGNESIUM ION × 457	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6；The Buffer pH was adjusted to 7.6 using KOH. cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.59 Å
8ZFG Structure of the Bacterial Ribosome with hypoxia-induced rRNA modifications Deposited 2024-05-07	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 54-meric	Chain k 1–144（144 aa）	Not recorded	MG MAGNESIUM ION × 471	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6；The Buffer pH was adjusted to 7.6 using KOH. cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.68 Å
8ZFI Structure of E.coli ribosome in complex with an engineered arrest peptide and trigger factor Deposited 2024-05-07	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 57-meric	Chain m 1–144（144 aa）	Not recorded	ZN ZINC ION × 1 PRO PROLINE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6；20 mM HEPES (pH 7.6), 140 mM NH4Cl, 25 mM MgCl2, 2 mM DTT cryo-EM vitrification conditions Cryogen NITROGEN	Resolution 2.90 Å
8ZTU 70S ribosome arrested by PepNL Deposited 2024-06-07	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric	Chain k 1–144（144 aa）	Not recorded	MG MAGNESIUM ION × 300 K POTASSIUM ION × 47 SPD SPERMIDINE × 7 PUT 1,4-DIAMINOBUTANE × 1 ZN ZINC ION × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.90 Å
8ZTV 70S ribosome arrested by PepNL with RF2 Deposited 2024-06-07	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 57-meric	Chain k 1–144（144 aa）	Not recorded	MG MAGNESIUM ION × 301 K POTASSIUM ION × 47 SPD SPERMIDINE × 7 PUT 1,4-DIAMINOBUTANE × 1 ZN ZINC ION × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.90 Å
9FBV 70S Escherichia coli ribosome with P-site initiatior tRNA. Deposited 2024-05-14	Different oligomeric state Different ligand/ion Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 54-meric	Chain k 1–144（144 aa）	Not recorded	ZN ZINC ION × 2 MG MAGNESIUM ION × 306 K POTASSIUM ION × 3	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE-PROPANE	Resolution 2.40 Å
9GFT Structure of the HrpA-bound E. coli disome, Class I Deposited 2024-08-12	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 105 PDB declaration: 116-meric	Chain Ag 1–144（144 aa） Chain Y 1–144（144 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.10 Å
9GGR Structure of the HrpA-bound E. coli disome, Class II Deposited 2024-08-14	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 106 PDB declaration: 117-meric	Chain Ag 1–144（144 aa） Chain Y 1–144（144 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.20 Å
9GHA Fusidic acid-locked Escherichia coli 70S ribosome with Staphylococcus aureus EF-G and a tRNA in pe/E chimeric state (CHI) Deposited 2024-08-15	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric	Chain k 1–144（144 aa）	Not recorded	ZN ZINC ION × 2 K POTASSIUM ION × 94 MG MAGNESIUM ION × 271 GDP GUANOSINE-5'-DIPHOSPHATE × 1 FUA FUSIDIC ACID × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.24 Å
9GHB Fusidic acid-locked Escherichia coli 70S ribosome with Staphylococcus aureus EF-G in post-translocational state (POST) Deposited 2024-08-15	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric	Chain k 1–144（144 aa）	Not recorded	ZN ZINC ION × 2 K POTASSIUM ION × 111 MG MAGNESIUM ION × 303 GDP GUANOSINE-5'-DIPHOSPHATE × 1 FUA FUSIDIC ACID × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.21 Å
9GHC Pre-release fusidic acid-locked Escherichia coli 70S ribosome with Staphylococus aureus EF-G and FusB (FusB-EF-G-70S) Deposited 2024-08-15	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 56-meric	Chain k 1–144（144 aa）	Not recorded	ZN ZINC ION × 3 K POTASSIUM ION × 109 MG MAGNESIUM ION × 301 GDP GUANOSINE-5'-DIPHOSPHATE × 1 FUA FUSIDIC ACID × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.79 Å
9GHD Escherichia coli 70S ribosome in complex with Staphylococcus aureus FusB-EF-G (FusB-EF-G-70S*) Deposited 2024-08-15	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 56-meric	Chain k 1–144（144 aa）	Not recorded	ZN ZINC ION × 2 K POTASSIUM ION × 112 MG MAGNESIUM ION × 303	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.41 Å
9GHE Staphylococcus aureus FusB bound to the small subunit of the Escherichia coli 70S ribosome (FusB-70S:SSU) Deposited 2024-08-15	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric	Chain k 1–144（144 aa）	Not recorded	ZN ZINC ION × 3 K POTASSIUM ION × 112 MG MAGNESIUM ION × 303	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 1.87 Å
9GHF Staphylococcus aureus FusB bound to the large subunit of the Escherichia coli 70S ribosome (FusB-70S:LSU) Deposited 2024-08-15	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric	Chain k 1–144（144 aa）	Not recorded	ZN ZINC ION × 3 K POTASSIUM ION × 112 MG MAGNESIUM ION × 303	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.40 Å
9GXX 70S ribosome with doublet-decoding tRNASer3 bound to A-site GCA codon Deposited 2024-10-01	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric	Chain k 1–144（144 aa）	Not recorded	MG MAGNESIUM ION × 288 SPD SPERMIDINE × 7 PUT 1,4-DIAMINOBUTANE × 7 SPM SPERMINE × 1 ZN ZINC ION × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5；HEPES-polymix buffer (pH-7.5) cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.61 Å
9H0L 70S ribosome with cognate tRNASer3 bound to A-site AGC codon Deposited 2024-10-08	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric	Chain k 1–144（144 aa）	Not recorded	MG MAGNESIUM ION × 314 SPD SPERMIDINE × 6 SPM SPERMINE × 1 PUT 1,4-DIAMINOBUTANE × 6 ZN ZINC ION × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5；HEPES-polymix buffer (pH-7.5) cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.49 Å
9H3K 50S subunit precursor d126_(L29)-/(L22)- Deposited 2024-10-17	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 8 PDB declaration: nonameric	Chain L 2–144（143 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 6.62 Å
9H3L 50S subunit precursor C_(L29)-/(L22)- Deposited 2024-10-17	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 12 PDB declaration: 13-meric	Chain L 2–144（143 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 5.84 Å
9H3M 50S subunit precursor C_(L22)- Deposited 2024-10-17	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 13 PDB declaration: 14-meric	Chain L 2–144（143 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 4.41 Å
9H3N 50S subunit precursor C_(L22)-~H61 Deposited 2024-10-17	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 12 PDB declaration: 13-meric	Chain L 2–144（143 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.69 Å
9H3O 50S subunit precursor C_(L22)-_GAC Deposited 2024-10-17	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 16 PDB declaration: 17-meric	Chain L 2–144（143 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 4.54 Å
9H3P 50S subunit precursor C-CP_(L22)- Deposited 2024-10-17	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 20 PDB declaration: 22-meric	Chain L 2–144（143 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 7.06 Å
9H3Q 50S subunit precursor C-CP_YjgA_(L22)-~H61 Deposited 2024-10-17	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 21 PDB declaration: 23-meric	Chain L 2–144（143 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 4.02 Å
9H3R 50S subunit precursor C-CP_YjgA_(L22)- Deposited 2024-10-17	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 23 PDB declaration: 25-meric	Chain L 2–144（143 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 4.12 Å
9H3S 50S subunit precursor C-CP_YjgA_L22 Deposited 2024-10-17	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 25 PDB declaration: 27-meric	Chain L 2–144（143 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 4.16 Å
9H3T 50S subunit precursor C_L2 Deposited 2024-10-17	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 19 PDB declaration: 20-meric	Chain L 2–144（143 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.85 Å
9H3U 50S subunit precursor C_H68 Deposited 2024-10-17	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 19 PDB declaration: 20-meric	Chain L 2–144（143 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.47 Å
9H3V 50S subunit precursor C-CP_L2-L28 Deposited 2024-10-17	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 23 PDB declaration: 25-meric	Chain L 2–144（143 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.55 Å
9H3W 50S subunit precursor C-CP_H68 Deposited 2024-10-17	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 23 PDB declaration: 25-meric	Chain L 2–144（143 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 5.38 Å
9H3X 50S subunit precursor C-CP_H68_L35 Deposited 2024-10-17	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 25 PDB declaration: 27-meric	Chain L 2–144（143 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 4.12 Å
9H3Y 50S subunit precursor 50S_(L16)- Deposited 2024-10-17	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 26 PDB declaration: 28-meric	Chain L 2–144（143 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.09 Å
9H3Z mature 50S subunit Deposited 2024-10-17	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 28 PDB declaration: 30-meric	Chain L 2–144（143 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.98 Å
9HA1 Pooled 50S subunit C_(L22)- precursor states supplemented with Api137 - Canonical PET exit Api137 Deposited 2024-11-01	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 14 PDB declaration: 15-meric	Chain L 2–144（143 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 4.17 Å
9HA2 Pooled 50S subunit C_(L22)- precursor states supplemented with Api137 - Alternative PET exit Api137 Deposited 2024-11-01	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 14 PDB declaration: 15-meric	Chain L 2–144（143 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 4.17 Å
9HA3 Pooled 50S subunit C_(L22)-~H61 precursor states supplemented with Api137 Deposited 2024-11-01	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 13 PDB declaration: 14-meric	Chain L 2–144（143 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.62 Å
9HA4 Pooled 50S subunit C-CP_(L22)- precursor states supplemented with Api137 Deposited 2024-11-01	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 19 PDB declaration: 21-meric	Chain L 2–144（143 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 4.26 Å
9HA5 Pooled 50S subunit C_L2 precursor states supplemented with Api137 Deposited 2024-11-01	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 18 PDB declaration: 19-meric	Chain L 2–144（143 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.30 Å
9HA6 mature 50S subunit supplemented with Api137 Deposited 2024-11-01	Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 30 PDB declaration: 32-meric	Chain L 2–144（143 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.08 Å
9HA7 Pooled 50S subunit C-CP_(L22)-~H61 precursor states supplemented with Api137 Deposited 2024-11-01	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 17 PDB declaration: 19-meric	Chain L 2–144（143 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 4.37 Å
9HAI Pooled 50S subunit C-CP_L2-L28 precursor states supplemented with Api137 Deposited 2024-11-04	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 17 PDB declaration: 18-meric	Chain L 2–144（143 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.01 Å
9HAL Pooled 50S subunit d126_(L29)-/(L22)- precursor states supplemented with Api137 Deposited 2024-11-04	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 8 PDB declaration: nonameric	Chain L 2–144（143 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 4.49 Å
9HAM C_(L29)-/(L22)- precursor supplemented with Api137 Deposited 2024-11-04	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 12 PDB declaration: 13-meric	Chain L 2–144（143 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 5.06 Å
9HUQ Structure of WT E.coli ribosome with complexed filament nascent chain at length 47, with P-site tRNA Deposited 2024-12-23	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 29 PDB declaration: 32-meric	Chain l 1–144（144 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.49 Å
9HUS Structure of WT E.coli ribosome with complexed filament nascent chain at length 31, with P-site tRNAs Deposited 2024-12-23	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 29 PDB declaration: 32-meric	Chain l 1–144（144 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.40 Å
9HY6 Structure of E.coli ribosome with nascent chain at linker length of 31 amino acids, with mRNA, P-site and A-site tRNAs Deposited 2025-01-09	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 29 PDB declaration: 34-meric	Chain l 1–144（144 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.60 Å
9HYM Structure of WT E.coli ribosome with complexed filament nascent chain at length 34, with mRNA, P-site and A-site tRNAs, and mRNA Deposited 2025-01-10	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 29 PDB declaration: 34-meric	Chain l 1–144（144 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.20 Å
9I3L Structure of E.coli ribosome with filamin mutant Y719E nascent chain at linker length of 47 amino acids, with tRNA Deposited 2025-01-23	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 29 PDB declaration: 32-meric	Chain l 1–144（144 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.10 Å
9JMK 50S Ribosomal Subunit precursor state III Deposited 2024-09-20	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 26 PDB declaration: 28-meric	Chain L 1–144（144 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 4.00 Å
9JNS 50S precursor - Erm complex (C-II) Deposited 2024-09-23	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 17 PDB declaration: octadecameric	Chain L 1–144（144 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 4.70 Å
9JPM Structure of the Bacterial Ribosome with human tRNA Lys(mcm5s2U34) and mRNA(AAA) Deposited 2024-09-26	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric	Chain k 1–144（144 aa）	Not recorded	MG MAGNESIUM ION × 391 ZN ZINC ION × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6；The Buffer pH was adjusted to 7.6 using KOH. cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.43 Å
9JPO Structure of the Bacterial Ribosome with human tRNA Lys(mcm5h2U34) and mRNA(AAA) Deposited 2024-09-26	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric	Chain k 1–144（144 aa）	Not recorded	MG MAGNESIUM ION × 448 ZN ZINC ION × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6；The Buffer pH was adjusted to 7.6 using KOH. cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.18 Å
9JPP Structure of the Bacterial Ribosome with human tRNA Lys(mcm5s2U34) and mRNA(AAG) Deposited 2024-09-26	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric	Chain k 1–144（144 aa）	Not recorded	MG MAGNESIUM ION × 443 ZN ZINC ION × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6；The Buffer pH was adjusted to 7.6 using KOH. cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.26 Å
9JQ2 Structure of the Bacterial Ribosome with human tRNA Lys(mcm5h2U34) and mRNA(AAG) Deposited 2024-09-27	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric	Chain k 1–144（144 aa）	Not recorded	MG MAGNESIUM ION × 438 ZN ZINC ION × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6；The Buffer pH was adjusted to 7.6 using KOH. cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.61 Å
9JSR 50S precursor - Erm complex (C-I) Deposited 2024-10-01	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 17 PDB declaration: octadecameric	Chain L 1–144（144 aa）	Not recorded	AN6 5'-{[(3S)-3-amino-3-carboxypropyl](ethyl)amino}-5'-deoxyadenosine × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 4.00 Å
9MKK Structure of arbekacin bound Escherichia coli 70S ribosome Deposited 2024-12-17	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 47 PDB declaration: 52-meric	Chain k 1–144（144 aa）	Not recorded	84G Arbekacin × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.20 Å
9MOR Damaged 70S ribosome with PrfH bound Deposited 2024-12-27	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 58-meric	Chain L 1–144（144 aa）	Not recorded	MG MAGNESIUM ION × 439 ZN ZINC ION × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.65 Å
9MQ4 Damaged 70S ribosome with PrfH bound Deposited 2025-01-01	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 58-meric	Chain L 1–144（144 aa）	Not recorded	MG MAGNESIUM ION × 439 ZN ZINC ION × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.78 Å
9N2B Impacts of ribosomal RNA sequence variation on gene expression and phenotype: Cryo-EM structure of the rrsB ribosome (BBB-70S) Deposited 2025-01-28	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 54-meric	Chain LO 1–144（144 aa）	Not recorded	PUT 1,4-DIAMINOBUTANE × 9 SPD SPERMIDINE × 5 MG MAGNESIUM ION × 276 K POTASSIUM ION × 56 ZN ZINC ION × 3 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 FME N-FORMYLMETHIONINE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.20 Å
9N2C Impacts of ribosomal RNA sequence variation on gene expression and phenotype: Cryo-EM structure of the rrsH ribosome (HBB-70S) Deposited 2025-01-28	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 54-meric	Chain LO 1–144（144 aa）	Not recorded	PUT 1,4-DIAMINOBUTANE × 10 SPD SPERMIDINE × 5 MG MAGNESIUM ION × 283 K POTASSIUM ION × 49 ZN ZINC ION × 3 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 FME N-FORMYLMETHIONINE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.40 Å
9NJV E. coli 70S initiation complex (bL33 absent) Deposited 2025-02-28	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 53-meric	Chain 15 1–144（144 aa）	Not recorded	MG MAGNESIUM ION × 152 FME N-FORMYLMETHIONINE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.10 Å
9NKL E. coli 70S initiation complex Deposited 2025-03-01	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 54-meric	Chain 15 1–144（144 aa）	Not recorded	MG MAGNESIUM ION × 161	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.30 Å
9NL6 E. coli initiation complex with EQ2-YbiT in Non-hydrolytic 1/PtIM(a) conformation Deposited 2025-03-02	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric	Chain 15 1–144（144 aa）	Not recorded	MG MAGNESIUM ION × 252 FME N-FORMYLMETHIONINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 NA SODIUM ION × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.20 Å
9NL7 E. coli initiation complex with EQ2-YbiT in Non-hydrolytic 2/PtIM(b) conformation Deposited 2025-03-02	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 56-meric	Chain 15 1–144（144 aa）	Not recorded	MG MAGNESIUM ION × 250 FME N-FORMYLMETHIONINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 NA SODIUM ION × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.30 Å
9NLF E. coli initiation complex with EQ2-YbiT in Hydrolytic 2/PtIM(a) conformation Deposited 2025-03-03	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 56-meric	Chain 15 1–144（144 aa）	Not recorded	MG MAGNESIUM ION × 245 FME N-FORMYLMETHIONINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 NA SODIUM ION × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.20 Å
9NLJ E.coli Initiation complex with Uup-EQ2 Deposited 2025-03-03	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 56-meric	Chain 15 1–144（144 aa）	Not recorded	MG MAGNESIUM ION × 277 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 NA SODIUM ION × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.10 Å
9NLQ E. coli initiation complex with EQ2-YbiT in Hydrolytic 1/PtIM(a) conformation Deposited 2025-03-03	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 56-meric	Chain 15 1–144（144 aa）	Not recorded	MG MAGNESIUM ION × 241 FME N-FORMYLMETHIONINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 NA SODIUM ION × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.30 Å
9NLS E. coli initiation complex with EQ2-YbiT in Intermediate/PtIM(b) conformation Deposited 2025-03-03	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 56-meric	Chain 15 1–144（144 aa）	Not recorded	MG MAGNESIUM ION × 248 ZN ZINC ION × 1 FME N-FORMYLMETHIONINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 NA SODIUM ION × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.30 Å
9O2X Structure of WT E.coli ribosome 70S subunit with complexed with mRNA, P-site fMet-NH-tRNAfMet and A-site (S)-betahydroxyBocK charged NH-tRNAPyl Deposited 2025-04-04	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric	Chain k 1–144（144 aa）	Not recorded	ZN ZINC ION × 2 MG MAGNESIUM ION × 320 FME N-FORMYLMETHIONINE × 1 K POTASSIUM ION × 10 PAR PAROMOMYCIN × 1 SPD SPERMIDINE × 14 SPM SPERMINE × 1 A1B71 (2S)-6-[(tert-butoxycarbonyl)amino]-2-(hydroxymethyl)hexanoic acid × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.29 Å
9O2Y Structure of WT E.coli ribosome 70S subunit with complexed with mRNA, P-site fMet-NH-tRNAfMet and A-site (R) beta-2-hydroxy-BocLysine acid charged NH-tRNAPyl Deposited 2025-04-04	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric	Chain k 1–144（144 aa）	Not recorded	PAR PAROMOMYCIN × 1 MG MAGNESIUM ION × 320 SPD SPERMIDINE × 13 FME N-FORMYLMETHIONINE × 1 SPM SPERMINE × 1 K POTASSIUM ION × 10 ZN ZINC ION × 2 A1B70 (2R)-6-[(tert-butoxycarbonyl)amino]-2-(hydroxymethyl)hexanoic acid × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.14 Å
9PIH E. coli 70S ribosome bound to Doxycycline Deposited 2025-07-10	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 54-meric	Chain k 1–144（144 aa）	Not recorded	ZN ZINC ION × 1 MG MAGNESIUM ION × 276 K POTASSIUM ION × 92 DXT (4S,4AR,5S,5AR,6R,12AS)-4-(DIMETHYLAMINO)-3,5,10,12,12A-PENTAHYDROXY-6-METHYL-1,11-DIOXO-1,4,4A,5,5A,6,11,12A-OCTAHYDROTETRACENE-2-CARBOXAMIDE × 6	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.40 Å
9PII E. coli 70S ribosome bound to Sarecycline Deposited 2025-07-10	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 54-meric	Chain k 1–144（144 aa）	Not recorded	ZN ZINC ION × 1 MG MAGNESIUM ION × 281 K POTASSIUM ION × 100 V7A Sarecycline × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.40 Å
9PIJ E. coli 70S ribosome bound to Minocycline Deposited 2025-07-10	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 54-meric	Chain k 1–144（144 aa）	Not recorded	ZN ZINC ION × 1 MG MAGNESIUM ION × 340 K POTASSIUM ION × 148 MIY (4S,4AS,5AR,12AS)-4,7-BIS(DIMETHYLAMINO)-3,10,12,12A-TETRAHYDROXY-1,11-DIOXO-1,4,4A,5,5A,6,11,12A-OCTAHYDROTETRACENE-2- CARBOXAMIDE × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.20 Å
9Q87 Principles of ion binding to RNA inferred from the analysis of a 1.55 Angstrom resolution bacterial ribosome structure - Part I: Mg2+ Deposited 2025-02-23	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric	Chain k 1–144（144 aa）	Not recorded	K POTASSIUM ION × 235 MG MAGNESIUM ION × 415 CU1 COPPER (I) ION × 5 ZN ZINC ION × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 1.55 Å
9QQQ Cryo-EM structure of SKM-70S ribosomal stalled complex in the major state (vacant A-site, canon) Deposited 2025-04-01	Different oligomeric state Different ligand/ion Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric	Chain k 1–144（144 aa）	Not recorded	ZN ZINC ION × 2 A1JA7 [(2~{S},3~{S},4~{R},5~{R},6~{S})-4-[(2~{S},3~{R},4~{S},5~{R},6~{S})-5-acetamido-6-(hydroxymethyl)-4-[(2~{R},3~{R},4~{S},5~{S},6~{R})-6-(hydroxymethyl)-4-[(2~{S},3~{R},4~{R},5~{R},6~{R})-6-(hydroxymethyl)-5-methoxy-3,4-bis(oxidanyl)oxan-2-yl]oxy-3,5-bis(oxidanyl)oxan-2-yl]oxy-3-oxidanyl-oxan-2-yl]oxy-6-carbamimidamido-2-(hydroxymethyl)-5-oxidanyl-oxan-3-yl] carbamate × 1 MG MAGNESIUM ION × 312	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE-PROPANE	Resolution 2.26 Å
9QSJ Cryo-EM structure of SKM-70S ribosomal stalled complex in the A-tRNA positioned (Body open) state. Deposited 2025-04-05	Different oligomeric state Different ligand/ion Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric	Chain k 1–144（144 aa）	Not recorded	ZN ZINC ION × 2 A1JAI [(2~{S},3~{S},4~{R},5~{R},6~{S})-4-[(2~{S},3~{R},4~{S},5~{R},6~{S})-5-acetamido-6-(hydroxymethyl)-4-[(2~{R},3~{R},4~{S},5~{S},6~{R})-6-(hydroxymethyl)-4-[(2~{S},3~{R},4~{R},5~{R},6~{R})-6-(hydroxymethyl)-5-methoxy-3,4-bis(oxidanyl)oxan-2-yl]oxy-3,5-bis(oxidanyl)oxan-2-yl]oxy-3-oxidanyl-oxan-2-yl]oxy-2-(hydroxymethyl)-6-[[~{N}-[4-[(~{N}-methylcarbamimidoyl)amino]butyl]carbamimidoyl]amino]-5-oxidanyl-oxan-3-yl] carbamate × 1 MG MAGNESIUM ION × 311	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE-PROPANE	Resolution 2.62 Å
9R3A Structure of Stalled Beta-Galactosidase 70S Ribosome Nascent Chain Deposited 2025-05-03	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric	Chain k 1–144（144 aa）	Not recorded	ZN ZINC ION × 2 K POTASSIUM ION × 131 MG MAGNESIUM ION × 287 PRO PROLINE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.2 cryo-EM vitrification conditions Cryogen ETHANE-PROPANE	Resolution 2.86 Å
9RBF Structure of a stalled E. coli 70S RNC-NuoK-86 in complex with the membrane protein insertase SecYEG-YidC Deposited 2025-05-22	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 54 PDB declaration: 61-meric	Chain k 1–144（144 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY mmCIF provides none of the parsed conditions	Resolution 2.44 Å
9RFW Manikomycin bound to the Escherichia coli 50S ribosomal subunit Deposited 2025-06-05	Different oligomeric state Different ligand/ion Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 31 PDB declaration: 35-meric	Chain k 1–144（144 aa）	Not recorded	ZN ZINC ION × 2 MG MAGNESIUM ION × 213 K POTASSIUM ION × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE-PROPANE	Resolution 2.40 Å
9RJA Manikomycin bound to the Escherichia coli 70S ribosome Deposited 2025-06-12	Different oligomeric state Different ligand/ion Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 57-meric	Chain k 1–144（144 aa）	Not recorded	ZN ZINC ION × 2 MG MAGNESIUM ION × 309 K POTASSIUM ION × 2 PAR PAROMOMYCIN × 1 SPD SPERMIDINE × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE-PROPANE	Resolution 2.45 Å
9RTU Structure of the 70S-EF-G(P610L)-GDP-Pi ribosome complex with tRNAs in hybrid state 1 (H1-EF-G(P610L)-GDP-Pi) Deposited 2025-07-03	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 53 PDB declaration: 59-meric	Chain L 1–144（144 aa）	Not recorded	MG MAGNESIUM ION × 369 ZN ZINC ION × 2 NA SODIUM ION × 2 AM2 APRAMYCIN × 3 PO4 PHOSPHATE ION × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5；50 mM HEPES, 70 mM NH4Cl, 30 mM KCl, 3.5 mM MgCl2, 0.6 mM spermine, 0.4 mM spermidine cryo-EM vitrification conditions Cryogen ETHANE；Manual blotting & plunge-freezing	Resolution 3.00 Å
9RTV Structure of the 70S-EF-G(P610L)-GDP-Pi ribosome complex with tRNAs in hybrid state 2 (H2-EF-G(P610L)-GDP-Pi) Deposited 2025-07-03	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 53 PDB declaration: 59-meric	Chain L 1–144（144 aa）	Not recorded	MG MAGNESIUM ION × 369 ZN ZINC ION × 2 NA SODIUM ION × 2 AM2 APRAMYCIN × 3 GDP GUANOSINE-5'-DIPHOSPHATE × 1 PO4 PHOSPHATE ION × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5；50 mM HEPES, 70 mM NH4Cl, 30 mM KCl, 3.5 mM MgCl2, 0.6 mM spermine, 0.4 mM spermidine cryo-EM vitrification conditions Cryogen ETHANE；Manual blotting & plunge-freezing	Resolution 3.60 Å
9SLG Doxycycline Bound E. coli Ribosome with Rearranged Peptidyl Transferase Centre Deposited 2025-09-03	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 54-meric	Chain k 1–144（144 aa）	Not recorded	ZN ZINC ION × 2 MG MAGNESIUM ION × 447 K POTASSIUM ION × 77 DXT (4S,4AR,5S,5AR,6R,12AS)-4-(DIMETHYLAMINO)-3,5,10,12,12A-PENTAHYDROXY-6-METHYL-1,11-DIOXO-1,4,4A,5,5A,6,11,12A-OCTAHYDROTETRACENE-2-CARBOXAMIDE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5；BS100 ribosomal resuspension buffer cryo-EM vitrification conditions Cryogen ETHANE；Blot time 4 s, blot force 1, blot total 1	Resolution 2.16 Å
9SRO Cryo-EM structure of SKM-70S ribosomal stalled complex in the rotated state with hybrid tRNAs Deposited 2025-09-24	Different oligomeric state Different ligand/ion Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 54-meric	Chain k 1–144（144 aa）	Not recorded	ZN ZINC ION × 2 MG MAGNESIUM ION × 309 A1JAI [(2~{S},3~{S},4~{R},5~{R},6~{S})-4-[(2~{S},3~{R},4~{S},5~{R},6~{S})-5-acetamido-6-(hydroxymethyl)-4-[(2~{R},3~{R},4~{S},5~{S},6~{R})-6-(hydroxymethyl)-4-[(2~{S},3~{R},4~{R},5~{R},6~{R})-6-(hydroxymethyl)-5-methoxy-3,4-bis(oxidanyl)oxan-2-yl]oxy-3,5-bis(oxidanyl)oxan-2-yl]oxy-3-oxidanyl-oxan-2-yl]oxy-2-(hydroxymethyl)-6-[[~{N}-[4-[(~{N}-methylcarbamimidoyl)amino]butyl]carbamimidoyl]amino]-5-oxidanyl-oxan-3-yl] carbamate × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE-PROPANE	Resolution 2.60 Å
9SS4 pre-50S ribosomal subunit in rimM-KO with RsfS (-H68) Deposited 2025-09-25	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 33 PDB declaration: 35-meric	Chain BO 1–144（144 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.70 Å
9SS5 50S ribosomal subunit in rimM-KO with RsfS (+H68) Deposited 2025-09-25	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 32 PDB declaration: 34-meric	Chain BO 1–144（144 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.70 Å
9SS6 70S ribosome from RimM-KO Deposited 2025-09-25	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 55-meric	Chain BO 1–144（144 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.60 Å
9SYH E. coli 70S ribosome from deltaRlmE strain Deposited 2025-10-12	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric	Chain k 1–144（144 aa）	Not recorded	K POTASSIUM ION × 148 MG MAGNESIUM ION × 351 SR0 N~1~-(3-azaniumylpropyl)butane-1,4-diaminium × 6 PUT 1,4-DIAMINOBUTANE × 9 ZN ZINC ION × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5；HEPES-polymix buffer (pH-7.5) cryo-EM vitrification conditions Cryogen ETHANE	Resolution 1.89 Å
9T0Y E. coli 70S ribosome from delta-9 strain Deposited 2025-10-20	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric	Chain k 1–144（144 aa）	Not recorded	K POTASSIUM ION × 143 MG MAGNESIUM ION × 337 SR0 N~1~-(3-azaniumylpropyl)butane-1,4-diaminium × 6 PUT 1,4-DIAMINOBUTANE × 9 ZN ZINC ION × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5；HEPES-polymix buffer (pH-7.5) cryo-EM vitrification conditions Cryogen ETHANE	Resolution 1.87 Å
9T19 E. coli 70S ribosome from delta-RlmE strain, PTC class 0 Deposited 2025-10-21	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 10 PDB declaration: 14-meric	Chain k 1–144（144 aa）	Not recorded	SR0 N~1~-(3-azaniumylpropyl)butane-1,4-diaminium × 2 PUT 1,4-DIAMINOBUTANE × 1 MG MAGNESIUM ION × 99 K POTASSIUM ION × 42	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5；HEPES-polymix buffer (pH-7.5) cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.41 Å
9T1A E. coli 70S ribosome from delta-RlmE strain, PTC class 1 Deposited 2025-10-21	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 10 PDB declaration: 14-meric	Chain k 1–144（144 aa）	Not recorded	SR0 N~1~-(3-azaniumylpropyl)butane-1,4-diaminium × 2 PUT 1,4-DIAMINOBUTANE × 1 MG MAGNESIUM ION × 102 K POTASSIUM ION × 43	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5；HEPES-polymix buffer (pH-7.5) cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.39 Å
9T1B E. coli 70S ribosome from delta-RlmE strain, PTC class 2 Deposited 2025-10-21	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 10 PDB declaration: 14-meric	Chain k 1–144（144 aa）	Not recorded	SR0 N~1~-(3-azaniumylpropyl)butane-1,4-diaminium × 2 PUT 1,4-DIAMINOBUTANE × 1 MG MAGNESIUM ION × 102 K POTASSIUM ION × 43	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5；HEPES-polymix buffer (pH-7.5) cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.32 Å
9T1C E. coli 70S ribosome from delta-RlmE strain, PTC class 3 Deposited 2025-10-21	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 10 PDB declaration: 13-meric	Chain k 1–144（144 aa）	Not recorded	MG MAGNESIUM ION × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5；HEPES-polymix buffer (pH-7.5) cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.07 Å
9T1E E. coli 70S ribosome from delta-RlmE strain, PTC class 4 Deposited 2025-10-21	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 10 PDB declaration: 14-meric	Chain k 1–144（144 aa）	Not recorded	SR0 N~1~-(3-azaniumylpropyl)butane-1,4-diaminium × 2 PUT 1,4-DIAMINOBUTANE × 1 MG MAGNESIUM ION × 101 K POTASSIUM ION × 43	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5；HEPES-polymix buffer (pH-7.5) cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.28 Å
9T1F E. coli 70S ribosome from delta-RlmE strain, PTC class 5 Deposited 2025-10-21	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 10 PDB declaration: 14-meric	Chain k 1–144（144 aa）	Not recorded	SR0 N~1~-(3-azaniumylpropyl)butane-1,4-diaminium × 2 PUT 1,4-DIAMINOBUTANE × 1 MG MAGNESIUM ION × 102 K POTASSIUM ION × 40	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5；HEPES-polymix buffer (pH-7.5) cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.32 Å
9T1G E. coli 70S ribosome from delta-RlmE strain, PTC class 6 Deposited 2025-10-21	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 10 PDB declaration: 13-meric	Chain k 1–144（144 aa）	Not recorded	SR0 N~1~-(3-azaniumylpropyl)butane-1,4-diaminium × 2 PUT 1,4-DIAMINOBUTANE × 1 MG MAGNESIUM ION × 100 K POTASSIUM ION × 42	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5；HEPES-polymix buffer (pH-7.5) cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.29 Å
9T1H E. coli 70S ribosome from delta-RlmE strain, PTC class 7 Deposited 2025-10-21	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 10 PDB declaration: 14-meric	Chain k 1–144（144 aa）	Not recorded	SR0 N~1~-(3-azaniumylpropyl)butane-1,4-diaminium × 2 PUT 1,4-DIAMINOBUTANE × 1 MG MAGNESIUM ION × 102 K POTASSIUM ION × 43	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5；HEPES-polymix buffer (pH-7.5) cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.33 Å
9T1I E. coli 70S ribosome from delta-RlmE strain, PTC class 8 Deposited 2025-10-21	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 10 PDB declaration: 13-meric	Chain k 1–144（144 aa）	Not recorded	SR0 N~1~-(3-azaniumylpropyl)butane-1,4-diaminium × 2 PUT 1,4-DIAMINOBUTANE × 1 MG MAGNESIUM ION × 102 K POTASSIUM ION × 43	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5；HEPES-polymix buffer (pH-7.5) cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.30 Å
9T1J E. coli 70S ribosome from delta-RlmE strain, PTC class 9 Deposited 2025-10-21	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 10 PDB declaration: 14-meric	Chain k 1–144（144 aa）	Not recorded	SR0 N~1~-(3-azaniumylpropyl)butane-1,4-diaminium × 2 PUT 1,4-DIAMINOBUTANE × 1 MG MAGNESIUM ION × 102 K POTASSIUM ION × 43	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5；HEPES-polymix buffer (pH-7.5) cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.23 Å
9T2J E. coli 70S ribosome from delta-9 strain, PTC class 0 Deposited 2025-10-23	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 10 PDB declaration: 12-meric	Chain k 1–144（144 aa）	Not recorded	SR0 N~1~-(3-azaniumylpropyl)butane-1,4-diaminium × 2 PUT 1,4-DIAMINOBUTANE × 1 MG MAGNESIUM ION × 101 K POTASSIUM ION × 39	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5；HEPES-polymix buffer (pH-7.5) cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.02 Å
9T2K E. coli 70S ribosome from delta-9 strain, PTC class 1 Deposited 2025-10-23	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 10 PDB declaration: 14-meric	Chain k 1–144（144 aa）	Not recorded	SR0 N~1~-(3-azaniumylpropyl)butane-1,4-diaminium × 2 PUT 1,4-DIAMINOBUTANE × 1 MG MAGNESIUM ION × 101 K POTASSIUM ION × 40	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5；HEPES-polymix buffer (pH-7.5) cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.06 Å
9T2L E. coli 70S ribosome from delta-9 strain, PTC class 2 Deposited 2025-10-23	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 10 PDB declaration: 14-meric	Chain k 1–144（144 aa）	Not recorded	SR0 N~1~-(3-azaniumylpropyl)butane-1,4-diaminium × 2 PUT 1,4-DIAMINOBUTANE × 1 MG MAGNESIUM ION × 101 K POTASSIUM ION × 40	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5；HEPES-polymix buffer (pH-7.5) cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.07 Å
9T2M E. coli 70S ribosome from delta-9 strain, PTC class 3 Deposited 2025-10-23	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 10 PDB declaration: 13-meric	Chain k 1–144（144 aa）	Not recorded	SR0 N~1~-(3-azaniumylpropyl)butane-1,4-diaminium × 2 PUT 1,4-DIAMINOBUTANE × 1 MG MAGNESIUM ION × 101 K POTASSIUM ION × 39	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5；HEPES-polymix buffer (pH-7.5) cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.06 Å
9T2N E. coli 70S ribosome from delta-9 strain, PTC class 4 Deposited 2025-10-23	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 10 PDB declaration: 14-meric	Chain k 1–144（144 aa）	Not recorded	SR0 N~1~-(3-azaniumylpropyl)butane-1,4-diaminium × 2 PUT 1,4-DIAMINOBUTANE × 1 MG MAGNESIUM ION × 101 K POTASSIUM ION × 36	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5；HEPES-polymix buffer (pH-7.5) cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.06 Å
9T2O E. coli 70S ribosome from delta-9 strain, PTC class 5 Deposited 2025-10-23	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 10 PDB declaration: 13-meric	Chain k 1–144（144 aa）	Not recorded	SR0 N~1~-(3-azaniumylpropyl)butane-1,4-diaminium × 2 PUT 1,4-DIAMINOBUTANE × 1 MG MAGNESIUM ION × 101 K POTASSIUM ION × 39	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5；HEPES-polymix buffer (pH-7.5) cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.07 Å
9T2P E. coli 70S ribosome from delta-9 strain, PTC class 6 Deposited 2025-10-23	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 10 PDB declaration: 14-meric	Chain k 1–144（144 aa）	Not recorded	SR0 N~1~-(3-azaniumylpropyl)butane-1,4-diaminium × 2 PUT 1,4-DIAMINOBUTANE × 1 MG MAGNESIUM ION × 101 K POTASSIUM ION × 36	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5；HEPES-polymix buffer (pH-7.5) cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.08 Å
9T2Q E. coli 70S ribosome from delta-9 strain, PTC class 7 Deposited 2025-10-23	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 10 PDB declaration: 14-meric	Chain k 1–144（144 aa）	Not recorded	SR0 N~1~-(3-azaniumylpropyl)butane-1,4-diaminium × 2 PUT 1,4-DIAMINOBUTANE × 1 MG MAGNESIUM ION × 101 K POTASSIUM ION × 40	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5；HEPES-polymix buffer (pH-7.5) cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.08 Å
9T2R E. coli 70S ribosome from delta-9 strain, PTC class 8 Deposited 2025-10-23	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 10 PDB declaration: 13-meric	Chain k 1–144（144 aa）	Not recorded	SR0 N~1~-(3-azaniumylpropyl)butane-1,4-diaminium × 2 PUT 1,4-DIAMINOBUTANE × 1 MG MAGNESIUM ION × 101 K POTASSIUM ION × 40	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5；HEPES-polymix buffer (pH-7.5) cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.22 Å
9T2S E. coli 70S ribosome from delta-9 strain, PTC class 9 Deposited 2025-10-23	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 10 PDB declaration: 14-meric	Chain k 1–144（144 aa）	Not recorded	SR0 N~1~-(3-azaniumylpropyl)butane-1,4-diaminium × 2 PUT 1,4-DIAMINOBUTANE × 1 MG MAGNESIUM ION × 100 K POTASSIUM ION × 39	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5；HEPES-polymix buffer (pH-7.5) cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.07 Å
9T6M E. coli 70S ribosome from delta-10 strain Deposited 2025-11-07	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric	Chain k 1–144（144 aa）	Not recorded	K POTASSIUM ION × 132 MG MAGNESIUM ION × 316 SR0 N~1~-(3-azaniumylpropyl)butane-1,4-diaminium × 5 PUT 1,4-DIAMINOBUTANE × 8 ZN ZINC ION × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5；HEPES-polymix buffer (pH-7.5) cryo-EM vitrification conditions Cryogen ETHANE	Resolution 1.87 Å
9T8K E. coli 70S ribosome from delta-10 strain, PTC class 0 Deposited 2025-11-13	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 9 PDB declaration: 11-meric	Chain k 1–144（144 aa）	Not recorded	SR0 N~1~-(3-azaniumylpropyl)butane-1,4-diaminium × 2 MG MAGNESIUM ION × 34 K POTASSIUM ION × 21	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5；HEPES-polymix buffer (pH-7.5) cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.18 Å
9T8L E. coli 70S ribosome from delta-10 strain, PTC class 1 Deposited 2025-11-13	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 9 PDB declaration: 11-meric	Chain k 1–144（144 aa）	Not recorded	SR0 N~1~-(3-azaniumylpropyl)butane-1,4-diaminium × 2 MG MAGNESIUM ION × 34 K POTASSIUM ION × 21	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5；HEPES-polymix buffer (pH-7.5) cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.21 Å
9T8M E. coli 70S ribosome from delta-10 strain, PTC class 2 Deposited 2025-11-13	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 9 PDB declaration: 11-meric	Chain k 1–144（144 aa）	Not recorded	SR0 N~1~-(3-azaniumylpropyl)butane-1,4-diaminium × 2 MG MAGNESIUM ION × 34 K POTASSIUM ION × 21	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5；HEPES-polymix buffer (pH-7.5) cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.20 Å
9T8N E. coli 70S ribosome from delta-10 strain, PTC class 3 Deposited 2025-11-13	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 9 PDB declaration: 13-meric	Chain k 1–144（144 aa）	Not recorded	SR0 N~1~-(3-azaniumylpropyl)butane-1,4-diaminium × 2 MG MAGNESIUM ION × 33 K POTASSIUM ION × 21	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5；HEPES-polymix buffer (pH-7.5) cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.22 Å
9T8O E. coli 70S ribosome from delta-10 strain, PTC class 4 Deposited 2025-11-13	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 9 PDB declaration: 11-meric	Chain k 1–144（144 aa）	Not recorded	SR0 N~1~-(3-azaniumylpropyl)butane-1,4-diaminium × 2 MG MAGNESIUM ION × 34 K POTASSIUM ION × 21	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5；HEPES-polymix buffer (pH-7.5) cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.19 Å
9T8P E. coli 70S ribosome from delta-10 strain, PTC class 5 Deposited 2025-11-13	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 9 PDB declaration: 12-meric	Chain k 1–144（144 aa）	Not recorded	SR0 N~1~-(3-azaniumylpropyl)butane-1,4-diaminium × 2 MG MAGNESIUM ION × 34 K POTASSIUM ION × 21	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5；HEPES-polymix buffer (pH-7.5) cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.22 Å
9T8Q E. coli 70S ribosome from delta-10 strain, PTC class 6 Deposited 2025-11-13	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 9 PDB declaration: 12-meric	Chain k 1–144（144 aa）	Not recorded	SR0 N~1~-(3-azaniumylpropyl)butane-1,4-diaminium × 2 MG MAGNESIUM ION × 34 K POTASSIUM ION × 21	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5；HEPES-polymix buffer (pH-7.5) cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.23 Å
9T8R E. coli 70S ribosome from delta-10 strain, PTC class 7 Deposited 2025-11-13	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 9 PDB declaration: 12-meric	Chain k 1–144（144 aa）	Not recorded	SR0 N~1~-(3-azaniumylpropyl)butane-1,4-diaminium × 2 MG MAGNESIUM ION × 34 K POTASSIUM ION × 21	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5；HEPES-polymix buffer (pH-7.5) cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.22 Å
9T8S E. coli 70S ribosome from delta-10 strain, PTC class 8 Deposited 2025-11-13	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 9 PDB declaration: 12-meric	Chain k 1–144（144 aa）	Not recorded	SR0 N~1~-(3-azaniumylpropyl)butane-1,4-diaminium × 2 MG MAGNESIUM ION × 34 K POTASSIUM ION × 21	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5；HEPES-polymix buffer (pH-7.5) cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.22 Å
9T8T E. coli 70S ribosome from delta-10 strain, PTC class 9 Deposited 2025-11-13	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 9 PDB declaration: 13-meric	Chain k 1–144（144 aa）	Not recorded	SR0 N~1~-(3-azaniumylpropyl)butane-1,4-diaminium × 1 MG MAGNESIUM ION × 34 K POTASSIUM ION × 21	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5；HEPES-polymix buffer (pH-7.5) cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.23 Å
9TEW DalDro bound to the initiating Escherichia coli 70S ribosome Deposited 2025-11-26	Different oligomeric state Different ligand/ion Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric	Chain k 1–144（144 aa）	Not recorded	ZN ZINC ION × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE-PROPANE	Resolution 2.90 Å
9TEX Dal2 bound to the Escherichia coli 70S ribosome Deposited 2025-11-26	Different oligomeric state Different ligand/ion Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric	Chain k 1–144（144 aa）	Not recorded	ZN ZINC ION × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE-PROPANE	Resolution 2.54 Å
9TEZ DalDroS bound to the Escherichia coli 50S ribosomal subunit Deposited 2025-11-26	Different oligomeric state Different ligand/ion Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 31 PDB declaration: 34-meric	Chain k 1–144（144 aa）	Not recorded	ZN ZINC ION × 2 SPM SPERMINE × 1 MG MAGNESIUM ION × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE-PROPANE	Resolution 2.88 Å
9TMI E. coli 70S ribosome with A- and P-site tRNA Deposited 2025-12-13	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric	Chain k 1–144（144 aa）	Not recorded	ZN ZINC ION × 2 MG MAGNESIUM ION × 258 SCM SPECTINOMYCIN × 1 VAL VALINE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.08 Å
9TNL E. coli 70S ribosome with A/P- and P/E-site tRNA Deposited 2025-12-16	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric	Chain k 1–144（144 aa）	Not recorded	ZN ZINC ION × 2 SCM SPECTINOMYCIN × 1 MG MAGNESIUM ION × 266 K POTASSIUM ION × 52 VAL VALINE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.11 Å
9TQA E. coli 70S ribosome, trapped conformational ground state of SSU-h44 apical loop, with A- and P-site tRNA Deposited 2025-12-19	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric	Chain k 1–144（144 aa）	Not recorded	ZN ZINC ION × 2 SCM SPECTINOMYCIN × 1 MG MAGNESIUM ION × 238 VAL VALINE × 1 K POTASSIUM ION × 6	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.06 Å
9WNQ Structure of E.coli ribosome in complex with an engineered arrest peptide Deposited 2025-09-05	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric	Chain m 1–144（144 aa）	Not recorded	ZN ZINC ION × 1 PRO PROLINE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6；20 mM HEPES (pH 7.6), 140 mM NH4Cl, 25 mM MgCl2, 2 mM DTT cryo-EM vitrification conditions Cryogen NITROGEN	Resolution 2.59 Å
9WNR Structure of E.coli ribosome in complex with an engineered arrest peptide and trigger factor Deposited 2025-09-05	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 57-meric	Chain m 1–144（144 aa）	Not recorded	ZN ZINC ION × 1 PRO PROLINE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6；20 mM HEPES (pH 7.6), 140 mM NH4Cl, 25 mM MgCl2, 2 mM DTT cryo-EM vitrification conditions Cryogen NITROGEN	Resolution 2.76 Å
9XFK In situ structure of bacterial 50S ribosomes Deposited 2025-10-29	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 29 PDB declaration: 31-meric	Chain T 1–144（144 aa）	Not recorded	MG MAGNESIUM ION × 166 NA SODIUM ION × 1 ZN ZINC ION × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.98 Å
9XFL In vitro structure of bacterial 50S ribosomes Deposited 2025-10-29	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 29 PDB declaration: 31-meric	Chain T 1–144（144 aa）	Not recorded	MG MAGNESIUM ION × 176 NA SODIUM ION × 1 ZN ZINC ION × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.7 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.16 Å
9Y79 Escherichia coli transcription-translation loosely coupled complex (TTC-LC^walked) containing mRNA with a 39 nt long spacer, NusG, NusA, and fMet-tRNAs in E-site and P-site Deposited 2025-09-09	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Other combination Heteromer；Protein × 60 PDB declaration: 68-meric	Chain u 1–144（144 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY mmCIF provides none of the parsed conditions	Resolution 3.20 Å
9ZTL Single Particle Cryo EM Analysis of a Ribosome Nascent Globin Complex Deposited 2025-12-23	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric	Chain k 1–144（144 aa）	Not recorded	ZN ZINC ION × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.91 Å

522 other PDB entries and 568 assemblies. Open the comparison page and filter oligomeric states

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UniProt name	RL15_ECOLI
Isoform	—
PDB entities	17
Chains and sequence ranges	Author chain k； PDBConstruct 1–144； UniProt 1–144

50S ribosomal protein L16

OrganismNot specified

UniProt A0A0H3EMH6

State in the Current Structure

Assembly	Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Associated Components	Method and Experimental Conditions	Structure Quality
1	Protein–RNA Heteromer Protein × 30 RNA 2 PDB declaration: 32-meric（32） Consistent with all polymer counts	Chain l； UniProt 1–136	Not recorded	50S ribosomal protein L33 × 1 （A0A3S5YPG8） 50S ribosomal protein L34 × 1 （A0A8X6EVB8） 50S ribosomal protein L35 × 1 （A0A8S0FT45） 50S ribosomal protein L36 × 1 （A0A2X1PTA3） 50S ribosomal protein L31 × 1 （C3SIV2） Drosocin1 × 1 （B4MNS1） 23S ribosomal RNA × 1 5S ribosomal RNA × 1 50S ribosomal protein L2 × 1 （A0A7L6YWH1） 50S ribosomal protein L3 × 1 （C3SQU2） 50S ribosomal protein L4 × 1 （D8A1N0） 50S ribosomal protein L5 × 1 （D6IEK6） 50S ribosomal protein L6 × 1 （C3SR17） 50S ribosomal protein L9 × 1 （C3SFP2） 50S ribosomal protein L13 × 1 （A0A656YGQ7） 50S ribosomal protein L14 × 1 （C3SQZ2） 50S ribosomal protein L15 × 1 （P02413） 50S ribosomal protein L17 × 1 （A0A1X3JBW6） 50S ribosomal protein L18 × 1 （A0A514EKV0） 50S ribosomal protein L19 × 1 （A0A370V6W8） 50S ribosomal protein L20 × 1 （A0A378BCJ4） 50S ribosomal protein L21 × 1 （A0A0E2L2H1） 50S ribosomal protein L22 × 1 （A0A0F6B7N1） 50S ribosomal protein L23 × 1 （D7Y3D8） 50S ribosomal protein L24 × 1 （S1E9P3） 50S ribosomal protein L25 × 1 （P68919） 50S ribosomal protein L27 × 1 （C3SSG7） 50S ribosomal protein L28 × 1 （C3SME2） 50S ribosomal protein L29 × 1 （P0A7M6） 50S ribosomal protein L30 × 1 （C3SR32） 50S ribosomal protein L32 × 1 （A0A657HW13） ZN ZINC ION × 2 A2G 2-acetamido-2-deoxy-alpha-D-galactopyranose × 1 MG MAGNESIUM ION × 216 SPM SPERMINE × 1	ELECTRON MICROSCOPY cryo-EM buffer：pH 7.4 cryo-EM vitrification conditions：Cryogen ETHANE-PROPANE	Resolution 2.10 Å

Other States of the Same Protein in the Database

Other PDB	Difference from Current Entry 8ANA	Assembly / Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Non-polymers	Method and Experimental Conditions	Structure Quality
8AKN Cryo-EM structure of the proline-rich antimicrobial peptide drosocin bound to the terminating ribosome Deposited 2022-07-30	Different oligomeric state Different ligand/ion Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 57-meric	Chain l 1–136（136 aa）	Not recorded	ZN ZINC ION × 2 A2G 2-acetamido-2-deoxy-alpha-D-galactopyranose × 1 MG MAGNESIUM ION × 305 SPM SPERMINE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE-PROPANE	Resolution 2.30 Å
8AM9 Cryo-EM structure of the proline-rich antimicrobial peptide drosocin bound to the elongating ribosome Deposited 2022-08-03	Different oligomeric state Different ligand/ion Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric	Chain l 1–136（136 aa）	Not recorded	ZN ZINC ION × 2 MG MAGNESIUM ION × 293 SPM SPERMINE × 1 A2G 2-acetamido-2-deoxy-alpha-D-galactopyranose × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE-PROPANE	Resolution 2.80 Å
8FTO E. coli 70S ribosome with an improved MS2 tag inserted in H98 Deposited 2023-01-12	Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 54-meric	Chain l 1–136（136 aa）	Non-standard monomer：Yes (specific site not provided by mmCIF)	MG MAGNESIUM ION × 284 FME N-FORMYLMETHIONINE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 1.85 Å
8QBT E. coli ApdP-stalled ribosomal complex Deposited 2023-08-25	Different oligomeric state Different ligand/ion Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 45 PDB declaration: 52-meric	Chain M 1–136（136 aa）	Not recorded	MG MAGNESIUM ION × 248 K POTASSIUM ION × 127 PRO PROLINE × 1 ZN ZINC ION × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE-PROPANE	Resolution 2.20 Å

4 other PDB entries and 4 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence

UniProt name	A0A0H3EMH6_ECO8N
Isoform	—
PDB entities	18
Chains and sequence ranges	Author chain l； PDBConstruct 1–136； UniProt 1–136

50S ribosomal protein L17

OrganismNot specified

UniProt A0A1X3JBW6

State in the Current Structure

Assembly	Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Associated Components	Method and Experimental Conditions	Structure Quality
1	Protein–RNA Heteromer Protein × 30 RNA 2 PDB declaration: 32-meric（32） Consistent with all polymer counts	Chain m； UniProt 1–127	Not recorded	50S ribosomal protein L33 × 1 （A0A3S5YPG8） 50S ribosomal protein L34 × 1 （A0A8X6EVB8） 50S ribosomal protein L35 × 1 （A0A8S0FT45） 50S ribosomal protein L36 × 1 （A0A2X1PTA3） 50S ribosomal protein L31 × 1 （C3SIV2） Drosocin1 × 1 （B4MNS1） 23S ribosomal RNA × 1 5S ribosomal RNA × 1 50S ribosomal protein L2 × 1 （A0A7L6YWH1） 50S ribosomal protein L3 × 1 （C3SQU2） 50S ribosomal protein L4 × 1 （D8A1N0） 50S ribosomal protein L5 × 1 （D6IEK6） 50S ribosomal protein L6 × 1 （C3SR17） 50S ribosomal protein L9 × 1 （C3SFP2） 50S ribosomal protein L13 × 1 （A0A656YGQ7） 50S ribosomal protein L14 × 1 （C3SQZ2） 50S ribosomal protein L15 × 1 （P02413） 50S ribosomal protein L16 × 1 （A0A0H3EMH6） 50S ribosomal protein L18 × 1 （A0A514EKV0） 50S ribosomal protein L19 × 1 （A0A370V6W8） 50S ribosomal protein L20 × 1 （A0A378BCJ4） 50S ribosomal protein L21 × 1 （A0A0E2L2H1） 50S ribosomal protein L22 × 1 （A0A0F6B7N1） 50S ribosomal protein L23 × 1 （D7Y3D8） 50S ribosomal protein L24 × 1 （S1E9P3） 50S ribosomal protein L25 × 1 （P68919） 50S ribosomal protein L27 × 1 （C3SSG7） 50S ribosomal protein L28 × 1 （C3SME2） 50S ribosomal protein L29 × 1 （P0A7M6） 50S ribosomal protein L30 × 1 （C3SR32） 50S ribosomal protein L32 × 1 （A0A657HW13） ZN ZINC ION × 2 A2G 2-acetamido-2-deoxy-alpha-D-galactopyranose × 1 MG MAGNESIUM ION × 216 SPM SPERMINE × 1	ELECTRON MICROSCOPY cryo-EM buffer：pH 7.4 cryo-EM vitrification conditions：Cryogen ETHANE-PROPANE	Resolution 2.10 Å

Other States of the Same Protein in the Database

Other PDB	Difference from Current Entry 8ANA	Assembly / Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Non-polymers	Method and Experimental Conditions	Structure Quality
8AKN Cryo-EM structure of the proline-rich antimicrobial peptide drosocin bound to the terminating ribosome Deposited 2022-07-30	Different oligomeric state Different ligand/ion Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 57-meric	Chain m 1–127（127 aa）	Not recorded	ZN ZINC ION × 2 A2G 2-acetamido-2-deoxy-alpha-D-galactopyranose × 1 MG MAGNESIUM ION × 305 SPM SPERMINE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE-PROPANE	Resolution 2.30 Å
8AM9 Cryo-EM structure of the proline-rich antimicrobial peptide drosocin bound to the elongating ribosome Deposited 2022-08-03	Different oligomeric state Different ligand/ion Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric	Chain m 1–127（127 aa）	Not recorded	ZN ZINC ION × 2 MG MAGNESIUM ION × 293 SPM SPERMINE × 1 A2G 2-acetamido-2-deoxy-alpha-D-galactopyranose × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE-PROPANE	Resolution 2.80 Å

2 other PDB entries and 2 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence

UniProt name	A0A1X3JBW6_ECOLX
Isoform	—
PDB entities	19
Chains and sequence ranges	Author chain m； PDBConstruct 1–127； UniProt 1–127

50S ribosomal protein L18

OrganismNot specified

UniProt A0A514EKV0

State in the Current Structure

Assembly	Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Associated Components	Method and Experimental Conditions	Structure Quality
1	Protein–RNA Heteromer Protein × 30 RNA 2 PDB declaration: 32-meric（32） Consistent with all polymer counts	Chain n； UniProt 1–117	Not recorded	50S ribosomal protein L33 × 1 （A0A3S5YPG8） 50S ribosomal protein L34 × 1 （A0A8X6EVB8） 50S ribosomal protein L35 × 1 （A0A8S0FT45） 50S ribosomal protein L36 × 1 （A0A2X1PTA3） 50S ribosomal protein L31 × 1 （C3SIV2） Drosocin1 × 1 （B4MNS1） 23S ribosomal RNA × 1 5S ribosomal RNA × 1 50S ribosomal protein L2 × 1 （A0A7L6YWH1） 50S ribosomal protein L3 × 1 （C3SQU2） 50S ribosomal protein L4 × 1 （D8A1N0） 50S ribosomal protein L5 × 1 （D6IEK6） 50S ribosomal protein L6 × 1 （C3SR17） 50S ribosomal protein L9 × 1 （C3SFP2） 50S ribosomal protein L13 × 1 （A0A656YGQ7） 50S ribosomal protein L14 × 1 （C3SQZ2） 50S ribosomal protein L15 × 1 （P02413） 50S ribosomal protein L16 × 1 （A0A0H3EMH6） 50S ribosomal protein L17 × 1 （A0A1X3JBW6） 50S ribosomal protein L19 × 1 （A0A370V6W8） 50S ribosomal protein L20 × 1 （A0A378BCJ4） 50S ribosomal protein L21 × 1 （A0A0E2L2H1） 50S ribosomal protein L22 × 1 （A0A0F6B7N1） 50S ribosomal protein L23 × 1 （D7Y3D8） 50S ribosomal protein L24 × 1 （S1E9P3） 50S ribosomal protein L25 × 1 （P68919） 50S ribosomal protein L27 × 1 （C3SSG7） 50S ribosomal protein L28 × 1 （C3SME2） 50S ribosomal protein L29 × 1 （P0A7M6） 50S ribosomal protein L30 × 1 （C3SR32） 50S ribosomal protein L32 × 1 （A0A657HW13） ZN ZINC ION × 2 A2G 2-acetamido-2-deoxy-alpha-D-galactopyranose × 1 MG MAGNESIUM ION × 216 SPM SPERMINE × 1	ELECTRON MICROSCOPY cryo-EM buffer：pH 7.4 cryo-EM vitrification conditions：Cryogen ETHANE-PROPANE	Resolution 2.10 Å

Other States of the Same Protein in the Database

Other PDB	Difference from Current Entry 8ANA	Assembly / Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Non-polymers	Method and Experimental Conditions	Structure Quality
8AM9 Cryo-EM structure of the proline-rich antimicrobial peptide drosocin bound to the elongating ribosome Deposited 2022-08-03	Different oligomeric state Different ligand/ion Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric	Chain n 1–117（117 aa）	Not recorded	ZN ZINC ION × 2 MG MAGNESIUM ION × 293 SPM SPERMINE × 1 A2G 2-acetamido-2-deoxy-alpha-D-galactopyranose × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE-PROPANE	Resolution 2.80 Å

1 other PDB entries and 1 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence

UniProt name	A0A514EKV0_9ENTR
Isoform	—
PDB entities	20
Chains and sequence ranges	Author chain n； PDBConstruct 1–117； UniProt 1–117

50S ribosomal protein L19

OrganismNot specified

UniProt A0A370V6W8

State in the Current Structure

Assembly	Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Associated Components	Method and Experimental Conditions	Structure Quality
1	Protein–RNA Heteromer Protein × 30 RNA 2 PDB declaration: 32-meric（32） Consistent with all polymer counts	Chain o； UniProt 1–115	Not recorded	50S ribosomal protein L33 × 1 （A0A3S5YPG8） 50S ribosomal protein L34 × 1 （A0A8X6EVB8） 50S ribosomal protein L35 × 1 （A0A8S0FT45） 50S ribosomal protein L36 × 1 （A0A2X1PTA3） 50S ribosomal protein L31 × 1 （C3SIV2） Drosocin1 × 1 （B4MNS1） 23S ribosomal RNA × 1 5S ribosomal RNA × 1 50S ribosomal protein L2 × 1 （A0A7L6YWH1） 50S ribosomal protein L3 × 1 （C3SQU2） 50S ribosomal protein L4 × 1 （D8A1N0） 50S ribosomal protein L5 × 1 （D6IEK6） 50S ribosomal protein L6 × 1 （C3SR17） 50S ribosomal protein L9 × 1 （C3SFP2） 50S ribosomal protein L13 × 1 （A0A656YGQ7） 50S ribosomal protein L14 × 1 （C3SQZ2） 50S ribosomal protein L15 × 1 （P02413） 50S ribosomal protein L16 × 1 （A0A0H3EMH6） 50S ribosomal protein L17 × 1 （A0A1X3JBW6） 50S ribosomal protein L18 × 1 （A0A514EKV0） 50S ribosomal protein L20 × 1 （A0A378BCJ4） 50S ribosomal protein L21 × 1 （A0A0E2L2H1） 50S ribosomal protein L22 × 1 （A0A0F6B7N1） 50S ribosomal protein L23 × 1 （D7Y3D8） 50S ribosomal protein L24 × 1 （S1E9P3） 50S ribosomal protein L25 × 1 （P68919） 50S ribosomal protein L27 × 1 （C3SSG7） 50S ribosomal protein L28 × 1 （C3SME2） 50S ribosomal protein L29 × 1 （P0A7M6） 50S ribosomal protein L30 × 1 （C3SR32） 50S ribosomal protein L32 × 1 （A0A657HW13） ZN ZINC ION × 2 A2G 2-acetamido-2-deoxy-alpha-D-galactopyranose × 1 MG MAGNESIUM ION × 216 SPM SPERMINE × 1	ELECTRON MICROSCOPY cryo-EM buffer：pH 7.4 cryo-EM vitrification conditions：Cryogen ETHANE-PROPANE	Resolution 2.10 Å

Other States of the Same Protein in the Database

Other PDB	Difference from Current Entry 8ANA	Assembly / Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Non-polymers	Method and Experimental Conditions	Structure Quality
8AKN Cryo-EM structure of the proline-rich antimicrobial peptide drosocin bound to the terminating ribosome Deposited 2022-07-30	Different oligomeric state Different ligand/ion Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 57-meric	Chain o 1–115（115 aa）	Not recorded	ZN ZINC ION × 2 A2G 2-acetamido-2-deoxy-alpha-D-galactopyranose × 1 MG MAGNESIUM ION × 305 SPM SPERMINE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE-PROPANE	Resolution 2.30 Å
8AM9 Cryo-EM structure of the proline-rich antimicrobial peptide drosocin bound to the elongating ribosome Deposited 2022-08-03	Different oligomeric state Different ligand/ion Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric	Chain o 1–115（115 aa）	Not recorded	ZN ZINC ION × 2 MG MAGNESIUM ION × 293 SPM SPERMINE × 1 A2G 2-acetamido-2-deoxy-alpha-D-galactopyranose × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE-PROPANE	Resolution 2.80 Å

2 other PDB entries and 2 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence

UniProt name	A0A370V6W8_9ESCH
Isoform	—
PDB entities	21
Chains and sequence ranges	Author chain o； PDBConstruct 1–115； UniProt 1–115

50S ribosomal protein L20

OrganismNot specified

UniProt A0A378BCJ4

State in the Current Structure

Assembly	Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Associated Components	Method and Experimental Conditions	Structure Quality
1	Protein–RNA Heteromer Protein × 30 RNA 2 PDB declaration: 32-meric（32） Consistent with all polymer counts	Chain p； UniProt 1–118	Not recorded	50S ribosomal protein L33 × 1 （A0A3S5YPG8） 50S ribosomal protein L34 × 1 （A0A8X6EVB8） 50S ribosomal protein L35 × 1 （A0A8S0FT45） 50S ribosomal protein L36 × 1 （A0A2X1PTA3） 50S ribosomal protein L31 × 1 （C3SIV2） Drosocin1 × 1 （B4MNS1） 23S ribosomal RNA × 1 5S ribosomal RNA × 1 50S ribosomal protein L2 × 1 （A0A7L6YWH1） 50S ribosomal protein L3 × 1 （C3SQU2） 50S ribosomal protein L4 × 1 （D8A1N0） 50S ribosomal protein L5 × 1 （D6IEK6） 50S ribosomal protein L6 × 1 （C3SR17） 50S ribosomal protein L9 × 1 （C3SFP2） 50S ribosomal protein L13 × 1 （A0A656YGQ7） 50S ribosomal protein L14 × 1 （C3SQZ2） 50S ribosomal protein L15 × 1 （P02413） 50S ribosomal protein L16 × 1 （A0A0H3EMH6） 50S ribosomal protein L17 × 1 （A0A1X3JBW6） 50S ribosomal protein L18 × 1 （A0A514EKV0） 50S ribosomal protein L19 × 1 （A0A370V6W8） 50S ribosomal protein L21 × 1 （A0A0E2L2H1） 50S ribosomal protein L22 × 1 （A0A0F6B7N1） 50S ribosomal protein L23 × 1 （D7Y3D8） 50S ribosomal protein L24 × 1 （S1E9P3） 50S ribosomal protein L25 × 1 （P68919） 50S ribosomal protein L27 × 1 （C3SSG7） 50S ribosomal protein L28 × 1 （C3SME2） 50S ribosomal protein L29 × 1 （P0A7M6） 50S ribosomal protein L30 × 1 （C3SR32） 50S ribosomal protein L32 × 1 （A0A657HW13） ZN ZINC ION × 2 A2G 2-acetamido-2-deoxy-alpha-D-galactopyranose × 1 MG MAGNESIUM ION × 216 SPM SPERMINE × 1	ELECTRON MICROSCOPY cryo-EM buffer：pH 7.4 cryo-EM vitrification conditions：Cryogen ETHANE-PROPANE	Resolution 2.10 Å

Other States of the Same Protein in the Database

Other PDB	Difference from Current Entry 8ANA	Assembly / Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Non-polymers	Method and Experimental Conditions	Structure Quality
8AKN Cryo-EM structure of the proline-rich antimicrobial peptide drosocin bound to the terminating ribosome Deposited 2022-07-30	Different oligomeric state Different ligand/ion Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 57-meric	Chain p 1–118（118 aa）	Not recorded	ZN ZINC ION × 2 A2G 2-acetamido-2-deoxy-alpha-D-galactopyranose × 1 MG MAGNESIUM ION × 305 SPM SPERMINE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE-PROPANE	Resolution 2.30 Å
8AM9 Cryo-EM structure of the proline-rich antimicrobial peptide drosocin bound to the elongating ribosome Deposited 2022-08-03	Different oligomeric state Different ligand/ion Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric	Chain p 1–118（118 aa）	Not recorded	ZN ZINC ION × 2 MG MAGNESIUM ION × 293 SPM SPERMINE × 1 A2G 2-acetamido-2-deoxy-alpha-D-galactopyranose × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE-PROPANE	Resolution 2.80 Å

2 other PDB entries and 2 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence

UniProt name	A0A378BCJ4_KLEPO
Isoform	—
PDB entities	22
Chains and sequence ranges	Author chain p； PDBConstruct 1–118； UniProt 1–118

50S ribosomal protein L21

OrganismNot specified

UniProt A0A0E2L2H1

State in the Current Structure

Assembly	Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Associated Components	Method and Experimental Conditions	Structure Quality
1	Protein–RNA Heteromer Protein × 30 RNA 2 PDB declaration: 32-meric（32） Consistent with all polymer counts	Chain q； UniProt 1–103	Not recorded	50S ribosomal protein L33 × 1 （A0A3S5YPG8） 50S ribosomal protein L34 × 1 （A0A8X6EVB8） 50S ribosomal protein L35 × 1 （A0A8S0FT45） 50S ribosomal protein L36 × 1 （A0A2X1PTA3） 50S ribosomal protein L31 × 1 （C3SIV2） Drosocin1 × 1 （B4MNS1） 23S ribosomal RNA × 1 5S ribosomal RNA × 1 50S ribosomal protein L2 × 1 （A0A7L6YWH1） 50S ribosomal protein L3 × 1 （C3SQU2） 50S ribosomal protein L4 × 1 （D8A1N0） 50S ribosomal protein L5 × 1 （D6IEK6） 50S ribosomal protein L6 × 1 （C3SR17） 50S ribosomal protein L9 × 1 （C3SFP2） 50S ribosomal protein L13 × 1 （A0A656YGQ7） 50S ribosomal protein L14 × 1 （C3SQZ2） 50S ribosomal protein L15 × 1 （P02413） 50S ribosomal protein L16 × 1 （A0A0H3EMH6） 50S ribosomal protein L17 × 1 （A0A1X3JBW6） 50S ribosomal protein L18 × 1 （A0A514EKV0） 50S ribosomal protein L19 × 1 （A0A370V6W8） 50S ribosomal protein L20 × 1 （A0A378BCJ4） 50S ribosomal protein L22 × 1 （A0A0F6B7N1） 50S ribosomal protein L23 × 1 （D7Y3D8） 50S ribosomal protein L24 × 1 （S1E9P3） 50S ribosomal protein L25 × 1 （P68919） 50S ribosomal protein L27 × 1 （C3SSG7） 50S ribosomal protein L28 × 1 （C3SME2） 50S ribosomal protein L29 × 1 （P0A7M6） 50S ribosomal protein L30 × 1 （C3SR32） 50S ribosomal protein L32 × 1 （A0A657HW13） ZN ZINC ION × 2 A2G 2-acetamido-2-deoxy-alpha-D-galactopyranose × 1 MG MAGNESIUM ION × 216 SPM SPERMINE × 1	ELECTRON MICROSCOPY cryo-EM buffer：pH 7.4 cryo-EM vitrification conditions：Cryogen ETHANE-PROPANE	Resolution 2.10 Å

Other States of the Same Protein in the Database

Other PDB	Difference from Current Entry 8ANA	Assembly / Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Non-polymers	Method and Experimental Conditions	Structure Quality
8AKN Cryo-EM structure of the proline-rich antimicrobial peptide drosocin bound to the terminating ribosome Deposited 2022-07-30	Different oligomeric state Different ligand/ion Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 57-meric	Chain q 1–103（103 aa）	Not recorded	ZN ZINC ION × 2 A2G 2-acetamido-2-deoxy-alpha-D-galactopyranose × 1 MG MAGNESIUM ION × 305 SPM SPERMINE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE-PROPANE	Resolution 2.30 Å
8AM9 Cryo-EM structure of the proline-rich antimicrobial peptide drosocin bound to the elongating ribosome Deposited 2022-08-03	Different oligomeric state Different ligand/ion Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric	Chain q 1–103（103 aa）	Not recorded	ZN ZINC ION × 2 MG MAGNESIUM ION × 293 SPM SPERMINE × 1 A2G 2-acetamido-2-deoxy-alpha-D-galactopyranose × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE-PROPANE	Resolution 2.80 Å

2 other PDB entries and 2 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence

UniProt name	A0A0E2L2H1_ECOU3
Isoform	—
PDB entities	23
Chains and sequence ranges	Author chain q； PDBConstruct 1–103； UniProt 1–103

50S ribosomal protein L22

OrganismNot specified

UniProt A0A0F6B7N1

State in the Current Structure

Assembly	Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Associated Components	Method and Experimental Conditions	Structure Quality
1	Protein–RNA Heteromer Protein × 30 RNA 2 PDB declaration: 32-meric（32） Consistent with all polymer counts	Chain r； UniProt 1–110	Not recorded	50S ribosomal protein L33 × 1 （A0A3S5YPG8） 50S ribosomal protein L34 × 1 （A0A8X6EVB8） 50S ribosomal protein L35 × 1 （A0A8S0FT45） 50S ribosomal protein L36 × 1 （A0A2X1PTA3） 50S ribosomal protein L31 × 1 （C3SIV2） Drosocin1 × 1 （B4MNS1） 23S ribosomal RNA × 1 5S ribosomal RNA × 1 50S ribosomal protein L2 × 1 （A0A7L6YWH1） 50S ribosomal protein L3 × 1 （C3SQU2） 50S ribosomal protein L4 × 1 （D8A1N0） 50S ribosomal protein L5 × 1 （D6IEK6） 50S ribosomal protein L6 × 1 （C3SR17） 50S ribosomal protein L9 × 1 （C3SFP2） 50S ribosomal protein L13 × 1 （A0A656YGQ7） 50S ribosomal protein L14 × 1 （C3SQZ2） 50S ribosomal protein L15 × 1 （P02413） 50S ribosomal protein L16 × 1 （A0A0H3EMH6） 50S ribosomal protein L17 × 1 （A0A1X3JBW6） 50S ribosomal protein L18 × 1 （A0A514EKV0） 50S ribosomal protein L19 × 1 （A0A370V6W8） 50S ribosomal protein L20 × 1 （A0A378BCJ4） 50S ribosomal protein L21 × 1 （A0A0E2L2H1） 50S ribosomal protein L23 × 1 （D7Y3D8） 50S ribosomal protein L24 × 1 （S1E9P3） 50S ribosomal protein L25 × 1 （P68919） 50S ribosomal protein L27 × 1 （C3SSG7） 50S ribosomal protein L28 × 1 （C3SME2） 50S ribosomal protein L29 × 1 （P0A7M6） 50S ribosomal protein L30 × 1 （C3SR32） 50S ribosomal protein L32 × 1 （A0A657HW13） ZN ZINC ION × 2 A2G 2-acetamido-2-deoxy-alpha-D-galactopyranose × 1 MG MAGNESIUM ION × 216 SPM SPERMINE × 1	ELECTRON MICROSCOPY cryo-EM buffer：pH 7.4 cryo-EM vitrification conditions：Cryogen ETHANE-PROPANE	Resolution 2.10 Å

Other States of the Same Protein in the Database

Other PDB	Difference from Current Entry 8ANA	Assembly / Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Non-polymers	Method and Experimental Conditions	Structure Quality
8AKN Cryo-EM structure of the proline-rich antimicrobial peptide drosocin bound to the terminating ribosome Deposited 2022-07-30	Different oligomeric state Different ligand/ion Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 57-meric	Chain r 1–110（110 aa）	Not recorded	ZN ZINC ION × 2 A2G 2-acetamido-2-deoxy-alpha-D-galactopyranose × 1 MG MAGNESIUM ION × 305 SPM SPERMINE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE-PROPANE	Resolution 2.30 Å
8AM9 Cryo-EM structure of the proline-rich antimicrobial peptide drosocin bound to the elongating ribosome Deposited 2022-08-03	Different oligomeric state Different ligand/ion Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric	Chain r 1–110（110 aa）	Not recorded	ZN ZINC ION × 2 MG MAGNESIUM ION × 293 SPM SPERMINE × 1 A2G 2-acetamido-2-deoxy-alpha-D-galactopyranose × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE-PROPANE	Resolution 2.80 Å

2 other PDB entries and 2 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence

UniProt name	A0A0F6B7N1_SALT1
Isoform	—
PDB entities	24
Chains and sequence ranges	Author chain r； PDBConstruct 1–110； UniProt 1–110

50S ribosomal protein L23

OrganismNot specified

UniProt D7Y3D8

State in the Current Structure

Assembly	Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Associated Components	Method and Experimental Conditions	Structure Quality
1	Protein–RNA Heteromer Protein × 30 RNA 2 PDB declaration: 32-meric（32） Consistent with all polymer counts	Chain s； UniProt 1–100	Not recorded	50S ribosomal protein L33 × 1 （A0A3S5YPG8） 50S ribosomal protein L34 × 1 （A0A8X6EVB8） 50S ribosomal protein L35 × 1 （A0A8S0FT45） 50S ribosomal protein L36 × 1 （A0A2X1PTA3） 50S ribosomal protein L31 × 1 （C3SIV2） Drosocin1 × 1 （B4MNS1） 23S ribosomal RNA × 1 5S ribosomal RNA × 1 50S ribosomal protein L2 × 1 （A0A7L6YWH1） 50S ribosomal protein L3 × 1 （C3SQU2） 50S ribosomal protein L4 × 1 （D8A1N0） 50S ribosomal protein L5 × 1 （D6IEK6） 50S ribosomal protein L6 × 1 （C3SR17） 50S ribosomal protein L9 × 1 （C3SFP2） 50S ribosomal protein L13 × 1 （A0A656YGQ7） 50S ribosomal protein L14 × 1 （C3SQZ2） 50S ribosomal protein L15 × 1 （P02413） 50S ribosomal protein L16 × 1 （A0A0H3EMH6） 50S ribosomal protein L17 × 1 （A0A1X3JBW6） 50S ribosomal protein L18 × 1 （A0A514EKV0） 50S ribosomal protein L19 × 1 （A0A370V6W8） 50S ribosomal protein L20 × 1 （A0A378BCJ4） 50S ribosomal protein L21 × 1 （A0A0E2L2H1） 50S ribosomal protein L22 × 1 （A0A0F6B7N1） 50S ribosomal protein L24 × 1 （S1E9P3） 50S ribosomal protein L25 × 1 （P68919） 50S ribosomal protein L27 × 1 （C3SSG7） 50S ribosomal protein L28 × 1 （C3SME2） 50S ribosomal protein L29 × 1 （P0A7M6） 50S ribosomal protein L30 × 1 （C3SR32） 50S ribosomal protein L32 × 1 （A0A657HW13） ZN ZINC ION × 2 A2G 2-acetamido-2-deoxy-alpha-D-galactopyranose × 1 MG MAGNESIUM ION × 216 SPM SPERMINE × 1	ELECTRON MICROSCOPY cryo-EM buffer：pH 7.4 cryo-EM vitrification conditions：Cryogen ETHANE-PROPANE	Resolution 2.10 Å

Other States of the Same Protein in the Database

Other PDB	Difference from Current Entry 8ANA	Assembly / Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Non-polymers	Method and Experimental Conditions	Structure Quality
8AKN Cryo-EM structure of the proline-rich antimicrobial peptide drosocin bound to the terminating ribosome Deposited 2022-07-30	Different oligomeric state Different ligand/ion Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 57-meric	Chain s 1–100（100 aa）	Not recorded	ZN ZINC ION × 2 A2G 2-acetamido-2-deoxy-alpha-D-galactopyranose × 1 MG MAGNESIUM ION × 305 SPM SPERMINE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE-PROPANE	Resolution 2.30 Å
8AM9 Cryo-EM structure of the proline-rich antimicrobial peptide drosocin bound to the elongating ribosome Deposited 2022-08-03	Different oligomeric state Different ligand/ion Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric	Chain s 1–100（100 aa）	Not recorded	ZN ZINC ION × 2 MG MAGNESIUM ION × 293 SPM SPERMINE × 1 A2G 2-acetamido-2-deoxy-alpha-D-galactopyranose × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE-PROPANE	Resolution 2.80 Å

2 other PDB entries and 2 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence

UniProt name	D7Y3D8_ECOM1
Isoform	—
PDB entities	25
Chains and sequence ranges	Author chain s； PDBConstruct 1–100； UniProt 1–100

50S ribosomal protein L24

OrganismNot specified

UniProt S1E9P3

State in the Current Structure

Assembly	Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Associated Components	Method and Experimental Conditions	Structure Quality
1	Protein–RNA Heteromer Protein × 30 RNA 2 PDB declaration: 32-meric（32） Consistent with all polymer counts	Chain t； UniProt 1–104	Not recorded	50S ribosomal protein L33 × 1 （A0A3S5YPG8） 50S ribosomal protein L34 × 1 （A0A8X6EVB8） 50S ribosomal protein L35 × 1 （A0A8S0FT45） 50S ribosomal protein L36 × 1 （A0A2X1PTA3） 50S ribosomal protein L31 × 1 （C3SIV2） Drosocin1 × 1 （B4MNS1） 23S ribosomal RNA × 1 5S ribosomal RNA × 1 50S ribosomal protein L2 × 1 （A0A7L6YWH1） 50S ribosomal protein L3 × 1 （C3SQU2） 50S ribosomal protein L4 × 1 （D8A1N0） 50S ribosomal protein L5 × 1 （D6IEK6） 50S ribosomal protein L6 × 1 （C3SR17） 50S ribosomal protein L9 × 1 （C3SFP2） 50S ribosomal protein L13 × 1 （A0A656YGQ7） 50S ribosomal protein L14 × 1 （C3SQZ2） 50S ribosomal protein L15 × 1 （P02413） 50S ribosomal protein L16 × 1 （A0A0H3EMH6） 50S ribosomal protein L17 × 1 （A0A1X3JBW6） 50S ribosomal protein L18 × 1 （A0A514EKV0） 50S ribosomal protein L19 × 1 （A0A370V6W8） 50S ribosomal protein L20 × 1 （A0A378BCJ4） 50S ribosomal protein L21 × 1 （A0A0E2L2H1） 50S ribosomal protein L22 × 1 （A0A0F6B7N1） 50S ribosomal protein L23 × 1 （D7Y3D8） 50S ribosomal protein L25 × 1 （P68919） 50S ribosomal protein L27 × 1 （C3SSG7） 50S ribosomal protein L28 × 1 （C3SME2） 50S ribosomal protein L29 × 1 （P0A7M6） 50S ribosomal protein L30 × 1 （C3SR32） 50S ribosomal protein L32 × 1 （A0A657HW13） ZN ZINC ION × 2 A2G 2-acetamido-2-deoxy-alpha-D-galactopyranose × 1 MG MAGNESIUM ION × 216 SPM SPERMINE × 1	ELECTRON MICROSCOPY cryo-EM buffer：pH 7.4 cryo-EM vitrification conditions：Cryogen ETHANE-PROPANE	Resolution 2.10 Å

Other States of the Same Protein in the Database

Other PDB	Difference from Current Entry 8ANA	Assembly / Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Non-polymers	Method and Experimental Conditions	Structure Quality
8AKN Cryo-EM structure of the proline-rich antimicrobial peptide drosocin bound to the terminating ribosome Deposited 2022-07-30	Different oligomeric state Different ligand/ion Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 57-meric	Chain t 1–104（104 aa）	Not recorded	ZN ZINC ION × 2 A2G 2-acetamido-2-deoxy-alpha-D-galactopyranose × 1 MG MAGNESIUM ION × 305 SPM SPERMINE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE-PROPANE	Resolution 2.30 Å
8AM9 Cryo-EM structure of the proline-rich antimicrobial peptide drosocin bound to the elongating ribosome Deposited 2022-08-03	Different oligomeric state Different ligand/ion Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric	Chain t 1–104（104 aa）	Not recorded	ZN ZINC ION × 2 MG MAGNESIUM ION × 293 SPM SPERMINE × 1 A2G 2-acetamido-2-deoxy-alpha-D-galactopyranose × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE-PROPANE	Resolution 2.80 Å
8G2U Time-resolved cryo-EM study of the 70S recycling by the HflX:control-apo-70S at 900ms Deposited 2023-02-06	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 51-meric	Chain U 2–104（103 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.00 Å
8G31 Time-resolved cryo-EM study of the 70S recycling by the HflX:2nd Intermediate Deposited 2023-02-06	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 52-meric	Chain U 2–104（103 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.20 Å
8G34 Time-resolved cryo-EM study of the 70S recycling by the HflX:1st intermediate Deposited 2023-02-06	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 52-meric	Chain U 2–104（103 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.20 Å
8G38 Time-resolved cryo-EM study of the 70S recycling by the HflX:3rd Intermediate Deposited 2023-02-07	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 52-meric	Chain U 2–104（103 aa）	Not recorded	GTP GUANOSINE-5'-TRIPHOSPHATE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.20 Å
8T5D Cryo-EM studies of the interplay between uS2 ribosomal protein and leaderless mRNA during bacterial translation initiation Deposited 2023-06-13	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 53-meric	Chain U 2–104（103 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.20 Å
8T5H Cryo-EM studies of the interplay between uS2 ribosomal protein and leaderless mRNA during bacterial translation initiation Deposited 2023-06-13	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 46 PDB declaration: 51-meric	Chain U 2–104（103 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.30 Å

8 other PDB entries and 8 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence

UniProt name	S1E9P3_ECOLX
Isoform	—
PDB entities	26
Chains and sequence ranges	Author chain t； PDBConstruct 1–104； UniProt 1–104

50S ribosomal protein L25

OrganismNot specified

UniProt P68919

State in the Current Structure

Assembly	Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Associated Components	Method and Experimental Conditions	Structure Quality
1	Protein–RNA Heteromer Protein × 30 RNA 2 PDB declaration: 32-meric（32） Consistent with all polymer counts	Chain u； UniProt 1–94	Not recorded	50S ribosomal protein L33 × 1 （A0A3S5YPG8） 50S ribosomal protein L34 × 1 （A0A8X6EVB8） 50S ribosomal protein L35 × 1 （A0A8S0FT45） 50S ribosomal protein L36 × 1 （A0A2X1PTA3） 50S ribosomal protein L31 × 1 （C3SIV2） Drosocin1 × 1 （B4MNS1） 23S ribosomal RNA × 1 5S ribosomal RNA × 1 50S ribosomal protein L2 × 1 （A0A7L6YWH1） 50S ribosomal protein L3 × 1 （C3SQU2） 50S ribosomal protein L4 × 1 （D8A1N0） 50S ribosomal protein L5 × 1 （D6IEK6） 50S ribosomal protein L6 × 1 （C3SR17） 50S ribosomal protein L9 × 1 （C3SFP2） 50S ribosomal protein L13 × 1 （A0A656YGQ7） 50S ribosomal protein L14 × 1 （C3SQZ2） 50S ribosomal protein L15 × 1 （P02413） 50S ribosomal protein L16 × 1 （A0A0H3EMH6） 50S ribosomal protein L17 × 1 （A0A1X3JBW6） 50S ribosomal protein L18 × 1 （A0A514EKV0） 50S ribosomal protein L19 × 1 （A0A370V6W8） 50S ribosomal protein L20 × 1 （A0A378BCJ4） 50S ribosomal protein L21 × 1 （A0A0E2L2H1） 50S ribosomal protein L22 × 1 （A0A0F6B7N1） 50S ribosomal protein L23 × 1 （D7Y3D8） 50S ribosomal protein L24 × 1 （S1E9P3） 50S ribosomal protein L27 × 1 （C3SSG7） 50S ribosomal protein L28 × 1 （C3SME2） 50S ribosomal protein L29 × 1 （P0A7M6） 50S ribosomal protein L30 × 1 （C3SR32） 50S ribosomal protein L32 × 1 （A0A657HW13） ZN ZINC ION × 2 A2G 2-acetamido-2-deoxy-alpha-D-galactopyranose × 1 MG MAGNESIUM ION × 216 SPM SPERMINE × 1	ELECTRON MICROSCOPY cryo-EM buffer：pH 7.4 cryo-EM vitrification conditions：Cryogen ETHANE-PROPANE	Resolution 2.10 Å

Other States of the Same Protein in the Database

Other PDB	Difference from Current Entry 8ANA	Assembly / Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Non-polymers	Method and Experimental Conditions	Structure Quality
10QM E.coli 50S ribosomal subunit bound to compound 48a Deposited 2026-02-01	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 29 PDB declaration: 31-meric	Chain d 1–94（94 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY mmCIF provides none of the parsed conditions	Resolution 2.49 Å
11BQ Escherichia coli 70S ribosome containing an evolved 16S rRNA (EC-S3.5) Deposited 2026-02-16	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric	Chain u 1–94（94 aa）	Not recorded	ZN ZINC ION × 2 MG MAGNESIUM ION × 307 SPD SPERMIDINE × 14 SPM SPERMINE × 1 C CYTIDINE-5'-MONOPHOSPHATE × 1 G GUANOSINE-5'-MONOPHOSPHATE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.00 Å
11DG Chimeric Escherichia coli 70S ribosome containing an evolved Vibrio cholerae 16S rRNA (VC-S4.4) Deposited 2026-02-18	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric	Chain u 1–94（94 aa）	Not recorded	ZN ZINC ION × 2 MG MAGNESIUM ION × 272 SPD SPERMIDINE × 14 SPM SPERMINE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.00 Å
11EL Chimeric Escherichia coli 70S ribosome containing an evolved 16S rRNA from Vibrio cholerae (VC-ST) Deposited 2026-02-19	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric	Chain u 1–94（94 aa）	Not recorded	ZN ZINC ION × 2 MG MAGNESIUM ION × 308 SPD SPERMIDINE × 16 SPM SPERMINE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.20 Å
11FV Chimeric Escherichia coli 70S ribosome containing an evolved 16S rRNA from Pseudomonas aeruginosa (PA-S3.3) Deposited 2026-02-21	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 52-meric	Chain d 1–94（94 aa）	Not recorded	MG MAGNESIUM ION × 307	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.90 Å
11GG Chimeric Escherichia coli 70S ribosome containing an evolved 16S rRNA from Pseudomonas aeruginosa (PA-ST) Deposited 2026-02-22	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 52-meric	Chain d 1–94（94 aa）	Not recorded	MG MAGNESIUM ION × 307	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.02 Å
1B75 SOLUTION STRUCTURE OF RIBOSOMAL PROTEIN L25 FROM ESCHERICHIA COLI Deposited 1999-01-27	Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric	Chain A 1–94（94 aa）	Not recorded	No recorded non-water small molecule	SOLUTION NMR NMR measurement conditions pH 7；298 K；Ionic strength (raw mmCIF value) 100 mM KCL；Pressure 1	Resolution not provided
1D6K NMR SOLUTION STRUCTURE OF THE 5S RRNA E-LOOP/L25 COMPLEX Deposited 1999-10-14	Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Monomer；Protein × 1 PDB declaration: dimeric	Chain A 1–94（94 aa）	Not recorded	No recorded non-water small molecule	SOLUTION NMR NMR measurement conditions pH 7.2；298 K；Ionic strength (raw mmCIF value) 100mM KCL；Pressure AMBIENT NMR measurement conditions pH 7.2；298 K；Ionic strength (raw mmCIF value) 100mM KCL；Pressure AMBIENT NMR measurement conditions pH 7.2；298 K；Ionic strength (raw mmCIF value) 100mM KCL；Pressure AMBIENT NMR measurement conditions pH 7.2；298 K；Ionic strength (raw mmCIF value) 100mM KCL；Pressure AMBIENT NMR measurement conditions pH 7.2；298 K；Ionic strength (raw mmCIF value) 100mM KCL；Pressure AMBIENT NMR measurement conditions pH 7.2；298 K；Ionic strength (raw mmCIF value) 100mM KCL；Pressure AMBIENT NMR sample composition 2MM L25 U-15N,13C/5SE NA; 20MM PHOSPHATE BUFFER; 100MM KCL NMR sample composition 2.2MM L25 U-15N,13C/5SE NA; 20MM PHOSPHATE BUFFER; 100MM KCL NMR sample composition 1.8MM L25 NA/5SE U-15N; 20MM PHOSPHATE BUFFER; 100MM KCL NMR sample composition 1MM L25 U-15N/5SE U-15N; 20MM PHOSPHATE BUFFER; 100MM KCL NMR sample composition 1.8MM L25 NA/5SE U-15N,13C; 20MM PHOSPHATE BUFFER; 100MM KCL NMR sample composition 1.7MM L25 NA/5SE U-15N,13C; 20MM PHOSPHATE BUFFER; 100MM KCL	Resolution not provided
1DFU CRYSTAL STRUCTURE OF E.COLI RIBOSOMAL PROTEIN L25 COMPLEXED WITH A 5S RRNA FRAGMENT AT 1.8 A RESOLUTION Deposited 1999-11-21	Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Monomer；Protein × 1 PDB declaration: trimeric	Chain P 1–94（94 aa）	Not recorded	MG MAGNESIUM ION × 5	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6；292 K；MPD, POTASSIUM CHLORIDE, MAGNESIUM CHLORIDE, CACODYLATE, pH 6.0, VAPOR DIFFUSION, SITTING DROP at 292K	Resolution 1.80 Å R-free 0.225
28UH E. coli 70S ribosome, trapped conformational ground state of SSU-h44 apical loop, with A/P- and P/E-site tRNA Deposited 2026-02-20	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric	Chain u 1–94（94 aa）	Not recorded	ZN ZINC ION × 2 MG MAGNESIUM ION × 274 K POTASSIUM ION × 65 SCM SPECTINOMYCIN × 1 VAL VALINE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.06 Å
28UI E. coli 70S ribosome, trapped conformational excited state of SSU-h44 apical loop, with A- and P-site tRNA Deposited 2026-02-20	Different oligomeric state Different ligand/ion Different experimental conditions	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric	Chain u 1–94（94 aa）	Not recorded	ZN ZINC ION × 2 SCM SPECTINOMYCIN × 1 MG MAGNESIUM ION × 270 VAL VALINE × 1 K POTASSIUM ION × 11	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.10 Å
28UJ E. coli 70S ribosome, trapped conformational excited state of SSU-h44 apical loop, with A/P- and P/E-site tRNA Deposited 2026-02-20	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric	Chain u 1–94（94 aa）	Not recorded	ZN ZINC ION × 2 MG MAGNESIUM ION × 251 SCM SPECTINOMYCIN × 1 K POTASSIUM ION × 47 VAL VALINE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.16 Å
28UK E. coli 70S ribosome, conformational ground state mutation in SSU-h41, with A- and P-site tRNA Deposited 2026-02-20	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric	Chain u 1–94（94 aa）	Not recorded	ZN ZINC ION × 2 MG MAGNESIUM ION × 227 K POTASSIUM ION × 74 SCM SPECTINOMYCIN × 1 VAL VALINE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.24 Å
28UL E. coli 70S ribosome, conformational ground state mutation in SSU-h41, with A/P- and P/E-site tRNA Deposited 2026-02-20	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric	Chain u 1–94（94 aa）	Not recorded	ZN ZINC ION × 2 MG MAGNESIUM ION × 212 K POTASSIUM ION × 77 SCM SPECTINOMYCIN × 1 VAL VALINE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.50 Å
28UM E. coli 70S ribosome, conformational excited state mutation in SSU-h41, with A- and P-site tRNA Deposited 2026-02-20	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric	Chain u 1–94（94 aa）	Not recorded	ZN ZINC ION × 2 MG MAGNESIUM ION × 238 K POTASSIUM ION × 109 SCM SPECTINOMYCIN × 1 VAL VALINE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.15 Å
28UN E. coli 70S ribosome, conformational excited state mutation in SSU-h41, with A/P- and P/E-site tRNA Deposited 2026-02-20	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric	Chain u 1–94（94 aa）	Not recorded	ZN ZINC ION × 2 MG MAGNESIUM ION × 183 K POTASSIUM ION × 65 SCM SPECTINOMYCIN × 1 VAL VALINE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.48 Å
2J28 MODEL OF E. COLI SRP BOUND TO 70S RNCS Deposited 2006-08-16	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 31 PDB declaration: 34-meric	Chain V 1–94（94 aa）	Not recorded	MG MAGNESIUM ION × 111	ELECTRON MICROSCOPY cryo-EM vitrification conditions Cryogen ETHANE	Resolution 9.50 Å
2RDO 50S subunit with EF-G(GDPNP) and RRF bound Deposited 2007-09-24	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 32 PDB declaration: 34-meric	Chain V 1–94（94 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer PolyMix；pH 7.5；PolyMix cryo-EM vitrification conditions Cryogen ETHANE；Rapid-freezing in liquid ethane by Vitrobot	Resolution 9.10 Å
30QZ E. coli 50S ribosome with peptidyl-tRNA and Hsp15 Deposited 2026-05-10	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 30 PDB declaration: 33-meric	Chain u 1–94（94 aa）	Not recorded	MG MAGNESIUM ION × 204 ZN ZINC ION × 1 ASP ASPARTIC ACID × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5；HEPES-polymix buffer (pH-7.5) cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.23 Å
30RA E. coli 70S ribosome with peptidyl-tRNA, mRNA and Hsp15 Deposited 2026-05-10	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric	Chain u 1–94（94 aa）	Not recorded	ZN ZINC ION × 2 MG MAGNESIUM ION × 281 ASP ASPARTIC ACID × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5；HEPES-polymix buffer (pH-7.5) cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.78 Å
30RB E. coli 70S ribosome with peptidyl-tRNA and Hsp15 Deposited 2026-05-10	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric	Chain u 1–94（94 aa）	Not recorded	ZN ZINC ION × 1 MG MAGNESIUM ION × 210 K POTASSIUM ION × 2 ASP ASPARTIC ACID × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5；HEPES-polymix buffer (pH-7.5) cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.92 Å
3BBX The Hsp15 protein fitted into the low resolution Cryo-EM map of the 50S.nc-tRNA.Hsp15 complex Deposited 2007-11-11	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 28 PDB declaration: 30-meric	Chain V 1–94（94 aa）	Not recorded	MG MAGNESIUM ION × 110	ELECTRON MICROSCOPY cryo-EM vitrification conditions Cryogen ETHANE	Resolution 10.00 Å
3J5L Structure of the E. coli 50S subunit with ErmBL nascent chain Deposited 2013-10-23	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 30 PDB declaration: 34-meric	Chain V 1–94（94 aa）	Not recorded	UNL UNKNOWN LIGAND × 1 ERY ERYTHROMYCIN A × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE；Plunged into liquid ethane (FEI VITROBOT MARK IV)	Resolution 6.60 Å
3J7Z Structure of the E. coli 50S subunit with ErmCL nascent chain Deposited 2014-08-27	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 32 PDB declaration: 35-meric	Chain V 1–94（94 aa）	Not recorded	ERY ERYTHROMYCIN A × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE；Plunged into liquid ethane (FEI VITROBOT MARK IV).	Resolution 3.90 Å
3J9Y Cryo-EM structure of tetracycline resistance protein TetM bound to a translating E.coli ribosome Deposited 2015-03-23	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 53 PDB declaration: 58-meric	Chain V 1–94（94 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer 50 mM HEPES-KOH, 100 mM KOAc, 25 mM Mg(OAc)2, 6 mM b-mercaptoethanol；pH 7.4；50 mM HEPES-KOH, 100 mM KOAc, 25 mM Mg(OAc)2, 6 mM b-mercaptoethanol cryo-EM vitrification conditions Cryogen ETHANE；Plunged into liquid ethane (FEI VITROBOT MARK IV)	Resolution 3.90 Å
3J9Z Activation of GTP Hydrolysis in mRNA-tRNA Translocation by Elongation Factor G Deposited 2015-03-27	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 53 PDB declaration: 58-meric	Chain LT 1–94（94 aa）	Not recorded	GTP GUANOSINE-5'-TRIPHOSPHATE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions 93 K；Cryogen ETHANE；Plunged into liquid ethane (FEI VITROBOT MARK IV)	Resolution 3.60 Å
3JA1 Activation of GTP Hydrolysis in mRNA-tRNA Translocation by Elongation Factor G Deposited 2015-03-30	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 53 PDB declaration: 58-meric	Chain LX 1–94（94 aa）	Not recorded	GTP GUANOSINE-5'-TRIPHOSPHATE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions 93 K；Cryogen ETHANE；Plunged into liquid ethane (FEI VITROBOT MARK IV)	Resolution 3.60 Å
3JBV Mechanisms of Ribosome Stalling by SecM at Multiple Elongation Steps Deposited 2015-10-16	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric	Chain w 1–94（94 aa）	Not recorded	CLM CHLORAMPHENICOL × 1	ELECTRON MICROSCOPY cryo-EM buffer 20mM HEPES, 50mM KOAc, 6mM Mg(OAc)2, 1mM DTT, 500 ug/ml chloramphenicol,0.05% Nikkol,0.5% pill/ml Complete EDTA-free Protease inhibitor cocktail,0.1 U/ml RNasin and 125mM sucrose；pH 7；20mM HEPES, 50mM KOAc, 6mM Mg(OAc)2, 1mM DTT, 500 ug/ml chloramphenicol,0.05% Nikkol,0.5% pill/ml Complete EDTA-free Protease inhibitor cocktail,0.1 U/ml RNasin and 125mM sucrose cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.32 Å
3JCD Structure of Escherichia coli EF4 in posttranslocational ribosomes (Post EF4) Deposited 2015-12-01	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 57-meric	Chain V 1–94（94 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.70 Å
3JCE Structure of Escherichia coli EF4 in pretranslocational ribosomes (Pre EF4) Deposited 2015-12-01	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 58-meric	Chain V 1–94（94 aa）	Not recorded	PHE PHENYLALANINE × 1 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1	ELECTRON MICROSCOPY cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.20 Å
3JCJ Structures of ribosome-bound initiation factor 2 reveal the mechanism of subunit association Deposited 2015-12-18	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 56-meric	Chain U 1–94（94 aa）	Not recorded	MG MAGNESIUM ION × 195 ZN ZINC ION × 1 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 FME N-FORMYLMETHIONINE × 1	ELECTRON MICROSCOPY cryo-EM buffer 20 mM HEPES-KOH, pH 7.5, 15 mM magnesium acetate, 150 mM potassium acetate, 4 mM 2-mercapthoethanol, 2 mM spermidine, 0.05 mM spermine；pH 7.5；20 mM HEPES-KOH, pH 7.5, 15 mM magnesium acetate, 150 mM potassium acetate, 4 mM 2-mercapthoethanol, 2 mM spermidine, 0.05 mM spermine cryo-EM vitrification conditions Blot for 2-4 seconds before plunging；Cryogen ETHANE；Blot for 2-4 seconds before plunging into liquid ethane (FEI VITROBOT MARK I).	Resolution 3.70 Å
3JCN Structures of ribosome-bound initiation factor 2 reveal the mechanism of subunit association: Initiation Complex I Deposited 2016-01-04	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric	Chain V 1–94（94 aa）	Not recorded	GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 FME N-FORMYLMETHIONINE × 1	ELECTRON MICROSCOPY cryo-EM buffer 20 mM HEPES-KOH, pH 7.5, 15 mM magnesium acetate, 150 mM potassium acetate, 4 mM 2-mercapthoethanol, 2 mM spermidine, 0.05 mM spermine；pH 7.5；20 mM HEPES-KOH, pH 7.5, 15 mM magnesium acetate, 150 mM potassium acetate, 4 mM 2-mercapthoethanol, 2 mM spermidine, 0.05 mM spermine cryo-EM vitrification conditions Blot for 2-4 seconds before plunging；Cryogen ETHANE；Blot for 2-4 seconds before plunging into liquid ethane (FEI VITROBOT MARK I).	Resolution 4.60 Å
4CSU Cryo-EM structures of the 50S ribosome subunit bound with ObgE Deposited 2014-03-10	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 31 PDB declaration: 33-meric	Chain W 1–94（94 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer 20MM TRIS-HCL, 100MM NH4CL,10MM MGCL2；pH 7.5；20MM TRIS-HCL, 100MM NH4CL,10MM MGCL2 cryo-EM vitrification conditions Cryogen ETHANE；LIQUID ETHANE	Resolution 5.50 Å
4U1U Crystal structure of the E. coli ribosome bound to quinupristin. Deposited 2014-07-16	Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 54-meric	Chain BV 1–94（94 aa）	Not recorded	MG MAGNESIUM ION × 271 ZN ZINC ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions EVAPORATION；pH 6.5；291 K；PEG8k, MPD	Resolution 2.95 Å R-free 0.282
4U1U Crystal structure of the E. coli ribosome bound to quinupristin. Deposited 2014-07-16	Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 53-meric	Chain DV 1–94（94 aa）	Not recorded	MG MAGNESIUM ION × 227 ZN ZINC ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions EVAPORATION；pH 6.5；291 K；PEG8k, MPD	Resolution 2.95 Å R-free 0.282
4U1V Crystal structure of the E. coli ribosome bound to linopristin. Deposited 2014-07-16	Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 54-meric	Chain BV 1–94（94 aa）	Not recorded	MG MAGNESIUM ION × 271 ZN ZINC ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions MICROBATCH；pH 6.5；291 K；PEG8k, MPD	Resolution 3.00 Å R-free 0.274
4U1V Crystal structure of the E. coli ribosome bound to linopristin. Deposited 2014-07-16	Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 53-meric	Chain DV 1–94（94 aa）	Not recorded	MG MAGNESIUM ION × 227 ZN ZINC ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions MICROBATCH；pH 6.5；291 K；PEG8k, MPD	Resolution 3.00 Å R-free 0.274
4U20 Crystal structure of the E. coli ribosome bound to flopristin. Deposited 2014-07-16	Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 53-meric	Chain BV 1–94（94 aa）	Not recorded	MG MAGNESIUM ION × 271 VIF Flopristin × 1 ZN ZINC ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions MICROBATCH；pH 6.5；291 K；PEG8k, MPD	Resolution 2.90 Å R-free 0.279
4U20 Crystal structure of the E. coli ribosome bound to flopristin. Deposited 2014-07-16	Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 52-meric	Chain DV 1–94（94 aa）	Not recorded	MG MAGNESIUM ION × 227 VIF Flopristin × 1 ZN ZINC ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions MICROBATCH；pH 6.5；291 K；PEG8k, MPD	Resolution 2.90 Å R-free 0.279
4U24 Crystal structure of the E. coli ribosome bound to dalfopristin. Deposited 2014-07-16	Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 53-meric	Chain BV 1–94（94 aa）	Not recorded	MG MAGNESIUM ION × 271 DOL 5-(2-DIETHYLAMINO-ETHANESULFONYL)-21-HYDROXY-10-ISOPROPYL-11,19-DIMETHYL-9,26-DIOXA-3,15,28-TRIAZA-TRICYCLO[23.2.1.00,255]OCTACOSA-1(27),12,17,19,25(28)-PENTAENE-2,8,14,23-TETRAONE × 1 ZN ZINC ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions MICROBATCH；pH 6.5；291 K；PEG8k, MPD	Resolution 2.90 Å R-free 0.264
4U24 Crystal structure of the E. coli ribosome bound to dalfopristin. Deposited 2014-07-16	Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 52-meric	Chain DV 1–94（94 aa）	Not recorded	MG MAGNESIUM ION × 226 DOL 5-(2-DIETHYLAMINO-ETHANESULFONYL)-21-HYDROXY-10-ISOPROPYL-11,19-DIMETHYL-9,26-DIOXA-3,15,28-TRIAZA-TRICYCLO[23.2.1.00,255]OCTACOSA-1(27),12,17,19,25(28)-PENTAENE-2,8,14,23-TETRAONE × 1 ZN ZINC ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions MICROBATCH；pH 6.5；291 K；PEG8k, MPD	Resolution 2.90 Å R-free 0.264
4U25 Crystal structure of the E. coli ribosome bound to virginiamycin M1. Deposited 2014-07-16	Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 53-meric	Chain BV 1–94（94 aa）	Not recorded	MG MAGNESIUM ION × 271 VIR VIRGINIAMYCIN M1 × 1 ZN ZINC ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions MICROBATCH；pH 6.5；291 K；PEG8k, MPD	Resolution 2.90 Å R-free 0.265
4U25 Crystal structure of the E. coli ribosome bound to virginiamycin M1. Deposited 2014-07-16	Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 52-meric	Chain DV 1–94（94 aa）	Not recorded	MG MAGNESIUM ION × 227 VIR VIRGINIAMYCIN M1 × 1 ZN ZINC ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions MICROBATCH；pH 6.5；291 K；PEG8k, MPD	Resolution 2.90 Å R-free 0.265
4U26 Crystal structure of the E. coli ribosome bound to dalfopristin and quinupristin. Deposited 2014-07-16	Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 54-meric	Chain BV 1–94（94 aa）	Not recorded	MG MAGNESIUM ION × 271 DOL 5-(2-DIETHYLAMINO-ETHANESULFONYL)-21-HYDROXY-10-ISOPROPYL-11,19-DIMETHYL-9,26-DIOXA-3,15,28-TRIAZA-TRICYCLO[23.2.1.00,255]OCTACOSA-1(27),12,17,19,25(28)-PENTAENE-2,8,14,23-TETRAONE × 1 ZN ZINC ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions MICROBATCH；pH 6.5；291 K；PEG8k, MPD	Resolution 2.80 Å R-free 0.271
4U26 Crystal structure of the E. coli ribosome bound to dalfopristin and quinupristin. Deposited 2014-07-16	Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 53-meric	Chain DV 1–94（94 aa）	Not recorded	MG MAGNESIUM ION × 227 DOL 5-(2-DIETHYLAMINO-ETHANESULFONYL)-21-HYDROXY-10-ISOPROPYL-11,19-DIMETHYL-9,26-DIOXA-3,15,28-TRIAZA-TRICYCLO[23.2.1.00,255]OCTACOSA-1(27),12,17,19,25(28)-PENTAENE-2,8,14,23-TETRAONE × 1 ZN ZINC ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions MICROBATCH；pH 6.5；291 K；PEG8k, MPD	Resolution 2.80 Å R-free 0.271
4U27 Crystal structure of the E. coli ribosome bound to flopristin and linopristin. Deposited 2014-07-16	Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 54-meric	Chain BV 1–94（94 aa）	Not recorded	MG MAGNESIUM ION × 271 VIF Flopristin × 1 ZN ZINC ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions MICROBATCH；pH 6.5；291 K；PEG8k, MPD	Resolution 2.80 Å R-free 0.260
4U27 Crystal structure of the E. coli ribosome bound to flopristin and linopristin. Deposited 2014-07-16	Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 53-meric	Chain DV 1–94（94 aa）	Not recorded	MG MAGNESIUM ION × 227 VIF Flopristin × 1 ZN ZINC ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions MICROBATCH；pH 6.5；291 K；PEG8k, MPD	Resolution 2.80 Å R-free 0.260
4V47 Real space refined coordinates of the 30S and 50S subunits fitted into the low resolution cryo-EM map of the EF-G.GTP state of E. coli 70S ribosome Deposited 2003-05-06	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 43 PDB declaration: 46-meric	Chain AT 1–94（94 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer Tris；pH 7.5；Tris cryo-EM vitrification conditions Rapid-freezing in liquid ethane	Resolution 12.30 Å
4V48 Real space refined coordinates of the 30S and 50S subunits fitted into the low resolution cryo-EM map of the initiation-like state of E. coli 70S ribosome Deposited 2003-05-06	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 44 PDB declaration: 48-meric	Chain AT 1–94（94 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer hepes；pH 7.5；hepes cryo-EM vitrification conditions Rapid-freezing in liquid ethane	Resolution 11.50 Å
4V4H Crystal structure of the bacterial ribosome from Escherichia coli in complex with the antibiotic kasugamyin at 3.5A resolution. Deposited 2006-08-04	Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 52-meric	Chain BV 1–94（94 aa）	Not recorded	KSG (1S,2R,3S,4R,5S,6S)-2,3,4,5,6-PENTAHYDROXYCYCLOHEXYL 2-AMINO-4-{[CARBOXY(IMINO)METHYL]AMINO}-2,3,4,6-TETRADEOXY-ALPHA-D-ARABINO-HEXOPYRANOSIDE × 1 MG MAGNESIUM ION × 170	X-RAY DIFFRACTION X-ray crystallization conditions pH 7.5；MPD, PEG 8000, MGCL2, NH4CL, SPERMINE, SPERMIDINE, TRIS-HCL, EDTA, PH 7.5, BATCH, TEMPERATURE 277K, pH 7.50	Resolution 3.46 Å R-free 0.331
4V4H Crystal structure of the bacterial ribosome from Escherichia coli in complex with the antibiotic kasugamyin at 3.5A resolution. Deposited 2006-08-04	Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 52-meric	Chain DV 1–94（94 aa）	Not recorded	KSG (1S,2R,3S,4R,5S,6S)-2,3,4,5,6-PENTAHYDROXYCYCLOHEXYL 2-AMINO-4-{[CARBOXY(IMINO)METHYL]AMINO}-2,3,4,6-TETRADEOXY-ALPHA-D-ARABINO-HEXOPYRANOSIDE × 1 MG MAGNESIUM ION × 173	X-RAY DIFFRACTION X-ray crystallization conditions pH 7.5；MPD, PEG 8000, MGCL2, NH4CL, SPERMINE, SPERMIDINE, TRIS-HCL, EDTA, PH 7.5, BATCH, TEMPERATURE 277K, pH 7.50	Resolution 3.46 Å R-free 0.331
4V4Q Crystal structure of the bacterial ribosome from Escherichia coli at 3.5 A resolution. Deposited 2005-08-30	Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 52-meric	Chain BV 1–94（94 aa）	Not recorded	MG MAGNESIUM ION × 170	X-RAY DIFFRACTION X-ray crystallization conditions batch；pH 7.5；277 K；MPD, PEG 8000, MgCl2, NH4Cl, spermine, spermidine, TRIS, EDTA, pH 7.5, batch, temperature 277K	Resolution 3.46 Å R-free 0.331
4V4Q Crystal structure of the bacterial ribosome from Escherichia coli at 3.5 A resolution. Deposited 2005-08-30	Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 52-meric	Chain DV 1–94（94 aa）	Not recorded	MG MAGNESIUM ION × 173	X-RAY DIFFRACTION X-ray crystallization conditions batch；pH 7.5；277 K；MPD, PEG 8000, MgCl2, NH4Cl, spermine, spermidine, TRIS, EDTA, pH 7.5, batch, temperature 277K	Resolution 3.46 Å R-free 0.331
4V4V Structure of a pre-translocational E. coli ribosome obtained by fitting atomic models for RNA and protein components into cryo-EM map EMD-1056 Deposited 2006-05-09	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 46 PDB declaration: 52-meric	Chain BT 1–94（94 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE；rapid-freezing in liquid ethane	Resolution 15.00 Å
4V4W Structure of a SecM-stalled E. coli ribosome complex obtained by fitting atomic models for RNA and protein components into cryo-EM map EMD-1143 Deposited 2006-05-09	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 46 PDB declaration: 52-meric	Chain BT 1–94（94 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE；rapid-freezing in liquid ethane	Resolution 15.00 Å
4V50 Crystal Structure of Ribosome with messenger RNA and the Anticodon stem-loop of P-site tRNA. Deposited 2006-08-16	Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 54-meric	Chain BV 1–94（94 aa）	Not recorded	MG MAGNESIUM ION × 181 ZN ZINC ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions batch；pH 7.5；277 K；MPD, PEG 8000, MGCL2, NH4CL, SPERMINE, SPERMIDINE, TRIS, EDTA, pH 7.5, batch, temperature 277K	Resolution 3.22 Å R-free 0.320
4V50 Crystal Structure of Ribosome with messenger RNA and the Anticodon stem-loop of P-site tRNA. Deposited 2006-08-16	Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 54-meric	Chain DV 1–94（94 aa）	Not recorded	MG MAGNESIUM ION × 177 ZN ZINC ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions batch；pH 7.5；277 K；MPD, PEG 8000, MGCL2, NH4CL, SPERMINE, SPERMIDINE, TRIS, EDTA, pH 7.5, batch, temperature 277K	Resolution 3.22 Å R-free 0.320
4V52 Crystal structure of the bacterial ribosome from Escherichia coli in complex with neomycin. Deposited 2007-06-15	Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 52-meric	Chain BV 1–94（94 aa）	Not recorded	NMY NEOMYCIN × 2 MG MAGNESIUM ION × 170 ZN ZINC ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions batch；pH 7.5；277 K；MPD, PEG 8000, MgCl2, NH4Cl, spermine, spermidine, TRIS, EDTA, pH 7.5, batch, temperature 277K	Resolution 3.21 Å R-free 0.309
4V52 Crystal structure of the bacterial ribosome from Escherichia coli in complex with neomycin. Deposited 2007-06-15	Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 52-meric	Chain DV 1–94（94 aa）	Not recorded	NMY NEOMYCIN × 2 MG MAGNESIUM ION × 173 ZN ZINC ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions batch；pH 7.5；277 K；MPD, PEG 8000, MgCl2, NH4Cl, spermine, spermidine, TRIS, EDTA, pH 7.5, batch, temperature 277K	Resolution 3.21 Å R-free 0.309
4V53 Crystal structure of the bacterial ribosome from Escherichia coli in complex with gentamicin. Deposited 2007-06-16	Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 52-meric	Chain BV 1–94（94 aa）	Not recorded	MG MAGNESIUM ION × 170 LLL (2R,3R,4R,5R)-2-((1S,2S,3R,4S,6R)-4,6-DIAMINO-3-((2R,3R,6S)-3-AMINO-6-(AMINOMETHYL)-TETRAHYDRO-2H-PYRAN-2-YLOXY)-2-HYDR OXYCYCLOHEXYLOXY)-5-METHYL-4-(METHYLAMINO)-TETRAHYDRO-2H-PYRAN-3,5-DIOL × 4 ZN ZINC ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions batch；pH 7.5；277 K；MPD, PEG 8000, MgCl2, NH4Cl, spermine, spermidine, TRIS, EDTA, pH 7.5, batch, temperature 277K	Resolution 3.54 Å R-free 0.320
4V53 Crystal structure of the bacterial ribosome from Escherichia coli in complex with gentamicin. Deposited 2007-06-16	Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 52-meric	Chain DV 1–94（94 aa）	Not recorded	MG MAGNESIUM ION × 173 LLL (2R,3R,4R,5R)-2-((1S,2S,3R,4S,6R)-4,6-DIAMINO-3-((2R,3R,6S)-3-AMINO-6-(AMINOMETHYL)-TETRAHYDRO-2H-PYRAN-2-YLOXY)-2-HYDR OXYCYCLOHEXYLOXY)-5-METHYL-4-(METHYLAMINO)-TETRAHYDRO-2H-PYRAN-3,5-DIOL × 4 ZN ZINC ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions batch；pH 7.5；277 K；MPD, PEG 8000, MgCl2, NH4Cl, spermine, spermidine, TRIS, EDTA, pH 7.5, batch, temperature 277K	Resolution 3.54 Å R-free 0.320
4V54 Crystal structure of the bacterial ribosome from Escherichia coli in complex with ribosome recycling factor (RRF). Deposited 2007-06-16	Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 53-meric	Chain BV 1–94（94 aa）	Not recorded	MG MAGNESIUM ION × 170 ZN ZINC ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions batch；pH 7.5；277 K；MPD, PEG 8000, MgCl2, NH4Cl, spermine, spermidine, TRIS, EDTA, pH 7.5, batch, temperature 277K	Resolution 3.30 Å R-free 0.304
4V54 Crystal structure of the bacterial ribosome from Escherichia coli in complex with ribosome recycling factor (RRF). Deposited 2007-06-16	Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 53-meric	Chain DV 1–94（94 aa）	Not recorded	MG MAGNESIUM ION × 173 ZN ZINC ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions batch；pH 7.5；277 K；MPD, PEG 8000, MgCl2, NH4Cl, spermine, spermidine, TRIS, EDTA, pH 7.5, batch, temperature 277K	Resolution 3.30 Å R-free 0.304
4V55 Crystal structure of the bacterial ribosome from Escherichia coli in complex with gentamicin and ribosome recycling factor (RRF). Deposited 2007-06-17	Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 53-meric	Chain BV 1–94（94 aa）	Not recorded	MG MAGNESIUM ION × 170 LLL (2R,3R,4R,5R)-2-((1S,2S,3R,4S,6R)-4,6-DIAMINO-3-((2R,3R,6S)-3-AMINO-6-(AMINOMETHYL)-TETRAHYDRO-2H-PYRAN-2-YLOXY)-2-HYDR OXYCYCLOHEXYLOXY)-5-METHYL-4-(METHYLAMINO)-TETRAHYDRO-2H-PYRAN-3,5-DIOL × 2 ZN ZINC ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions batch；pH 7.5；277 K；MPD, PEG 8000, MgCl2, NH4Cl, spermine, spermidine, TRIS, EDTA, pH 7.5, batch, temperature 277K	Resolution 4.00 Å R-free 0.305
4V55 Crystal structure of the bacterial ribosome from Escherichia coli in complex with gentamicin and ribosome recycling factor (RRF). Deposited 2007-06-17	Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 53-meric	Chain DV 1–94（94 aa）	Not recorded	MG MAGNESIUM ION × 173 LLL (2R,3R,4R,5R)-2-((1S,2S,3R,4S,6R)-4,6-DIAMINO-3-((2R,3R,6S)-3-AMINO-6-(AMINOMETHYL)-TETRAHYDRO-2H-PYRAN-2-YLOXY)-2-HYDR OXYCYCLOHEXYLOXY)-5-METHYL-4-(METHYLAMINO)-TETRAHYDRO-2H-PYRAN-3,5-DIOL × 2 ZN ZINC ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions batch；pH 7.5；277 K；MPD, PEG 8000, MgCl2, NH4Cl, spermine, spermidine, TRIS, EDTA, pH 7.5, batch, temperature 277K	Resolution 4.00 Å R-free 0.305
4V56 Crystal structure of the bacterial ribosome from Escherichia coli in complex with spectinomycin. Deposited 2007-07-21	Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 52-meric	Chain BV 1–94（94 aa）	Not recorded	MG MAGNESIUM ION × 170 SCM SPECTINOMYCIN × 1 ZN ZINC ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions batch；pH 7.5；277 K；MPD, PEG 8000, MgCl2, NH4Cl, spermine, spermidine, TRIS, EDTA, pH 7.5, batch, temperature 277K	Resolution 3.93 Å R-free 0.311
4V56 Crystal structure of the bacterial ribosome from Escherichia coli in complex with spectinomycin. Deposited 2007-07-21	Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 52-meric	Chain DV 1–94（94 aa）	Not recorded	MG MAGNESIUM ION × 170 SCM SPECTINOMYCIN × 1 ZN ZINC ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions batch；pH 7.5；277 K；MPD, PEG 8000, MgCl2, NH4Cl, spermine, spermidine, TRIS, EDTA, pH 7.5, batch, temperature 277K	Resolution 3.93 Å R-free 0.311
4V57 Crystal structure of the bacterial ribosome from Escherichia coli in complex with spectinomycin and neomycin. Deposited 2007-07-21	Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 52-meric	Chain BV 1–94（94 aa）	Not recorded	NMY NEOMYCIN × 2 MG MAGNESIUM ION × 170 SCM SPECTINOMYCIN × 1 ZN ZINC ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions batch；pH 7.5；277 K；MPD, PEG 8000, MgCl2, NH4Cl, spermine, spermidine, TRIS, EDTA, pH 7.5, batch, temperature 277K	Resolution 3.50 Å R-free 0.306
4V57 Crystal structure of the bacterial ribosome from Escherichia coli in complex with spectinomycin and neomycin. Deposited 2007-07-21	Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 52-meric	Chain DV 1–94（94 aa）	Not recorded	NMY NEOMYCIN × 2 MG MAGNESIUM ION × 170 SCM SPECTINOMYCIN × 1 ZN ZINC ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions batch；pH 7.5；277 K；MPD, PEG 8000, MgCl2, NH4Cl, spermine, spermidine, TRIS, EDTA, pH 7.5, batch, temperature 277K	Resolution 3.50 Å R-free 0.306
4V5B Structure of PDF binding helix in complex with the ribosome. Deposited 2007-11-22	Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Insufficient information Heteromer；Protein × 50 PDB declaration: 53-meric	Chain AV 1–94（94 aa）	Not recorded	MG MAGNESIUM ION × 170	X-RAY DIFFRACTION X-ray crystallization conditions pH 7.5；pH 7.5	Resolution 3.74 Å R-free 0.323
4V5B Structure of PDF binding helix in complex with the ribosome. Deposited 2007-11-22	Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 2 Insufficient information Heteromer；Protein × 49 PDB declaration: 52-meric	Chain CV 1–94（94 aa）	Not recorded	MG MAGNESIUM ION × 173	X-RAY DIFFRACTION X-ray crystallization conditions pH 7.5；pH 7.5	Resolution 3.74 Å R-free 0.323
4V5H E.Coli 70s Ribosome Stalled During Translation Of Tnac Leader Peptide. Deposited 2009-10-26	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 56-meric	Chain BW 1–94（94 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer 50 MM TRIS-HCL PH 8.0, 10MM MG-ACETATE, 50MM KCL, 10MM NH4CL, 2MM EGTA, 2MM L-TRYPTOPHAN, 10 MM DTT；pH 8；50 MM TRIS-HCL PH 8.0, 10MM MG-ACETATE, 50MM KCL, 10MM NH4CL, 2MM EGTA, 2MM L-TRYPTOPHAN, 10 MM DTT cryo-EM vitrification conditions Cryogen ETHANE；CRYOGEN- ETHANE,	Resolution 5.80 Å
4V5Y Crystal structure of the bacterial ribosome from Escherichia coli in complex with paromomycin and ribosome recycling factor (RRF). Deposited 2007-06-19	Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 53-meric	Chain BV 1–94（94 aa）	Not recorded	MG MAGNESIUM ION × 170 PAR PAROMOMYCIN × 2 ZN ZINC ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions batch；pH 7.5；277 K；MPD, PEG 8000, MgCl2, NH4Cl, spermine, spermidine, TRIS, EDTA, pH 7.5, batch, temperature 277K	Resolution 4.45 Å R-free 0.309
4V5Y Crystal structure of the bacterial ribosome from Escherichia coli in complex with paromomycin and ribosome recycling factor (RRF). Deposited 2007-06-19	Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 53-meric	Chain DV 1–94（94 aa）	Not recorded	MG MAGNESIUM ION × 173 PAR PAROMOMYCIN × 2 ZN ZINC ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions batch；pH 7.5；277 K；MPD, PEG 8000, MgCl2, NH4Cl, spermine, spermidine, TRIS, EDTA, pH 7.5, batch, temperature 277K	Resolution 4.45 Å R-free 0.309
4V64 Crystal structure of the bacterial ribosome from Escherichia coli in complex with hygromycin B. Deposited 2008-06-11	Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 52-meric	Chain BV 1–94（94 aa）	Not recorded	MG MAGNESIUM ION × 170 HYG HYGROMYCIN B × 1 ZN ZINC ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions batch；pH 7.5；277 K；MPD, PEG 8000, MgCl2, NH4Cl, spermine, spermidine, TRIS, EDTA, pH 7.5, batch, temperature 277K	Resolution 3.50 Å R-free 0.318
4V64 Crystal structure of the bacterial ribosome from Escherichia coli in complex with hygromycin B. Deposited 2008-06-11	Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 52-meric	Chain DV 1–94（94 aa）	Not recorded	MG MAGNESIUM ION × 173 HYG HYGROMYCIN B × 1 ZN ZINC ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions batch；pH 7.5；277 K；MPD, PEG 8000, MgCl2, NH4Cl, spermine, spermidine, TRIS, EDTA, pH 7.5, batch, temperature 277K	Resolution 3.50 Å R-free 0.318
4V65 Structure of the E. coli ribosome in the Pre-accommodation state Deposited 2008-08-03	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 57-meric	Chain BC 1–94（94 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 9.00 Å
4V66 Structure of the E. coli ribosome and the tRNAs in Post-accommodation state Deposited 2008-08-03	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 57-meric	Chain BC 1–94（94 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 9.00 Å
4V69 Ternary complex-bound E.coli 70S ribosome. Deposited 2008-12-11	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 58-meric	Chain BV 1–94（94 aa）	Not recorded	GDP GUANOSINE-5'-DIPHOSPHATE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE；Vitrification using FEI Vitrobot; blot 3 seconds before plunging with an offset of -1mm	Resolution 6.70 Å
4V6C Crystal structure of the E. coli 70S ribosome in an intermediate state of ratcheting Deposited 2009-06-27	Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 52-meric	Chain BV 1–94（94 aa）	Not recorded	MG MAGNESIUM ION × 184 ZN ZINC ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions MICROBATCH；291 K；4% PEG 8000, 4% MPD, 3.8 mM MgCl2, 380 mM NH4Cl, 5.5 mM putrescine, 5 mM spermidine, microbatch, temperature 291K	Resolution 3.19 Å R-free 0.252
4V6C Crystal structure of the E. coli 70S ribosome in an intermediate state of ratcheting Deposited 2009-06-27	Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 52-meric	Chain DV 1–94（94 aa）	Not recorded	MG MAGNESIUM ION × 179 ZN ZINC ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions MICROBATCH；291 K；4% PEG 8000, 4% MPD, 3.8 mM MgCl2, 380 mM NH4Cl, 5.5 mM putrescine, 5 mM spermidine, microbatch, temperature 291K	Resolution 3.19 Å R-free 0.252
4V6D Crystal structure of the E. coli 70S ribosome in an intermediate state of ratcheting Deposited 2009-06-27	Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 54-meric	Chain BV 1–94（94 aa）	Not recorded	MG MAGNESIUM ION × 184 ZN ZINC ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions MICROBATCH；pH 7；291 K；4% PEG 8000, 4% MPD, 3.8 mM MgCl2, 380 mM NH4Cl, 5.5 mM putrescine, 5 mM spermidine, pH 7.0, microbatch, temperature 291K	Resolution 3.81 Å R-free 0.253
4V6D Crystal structure of the E. coli 70S ribosome in an intermediate state of ratcheting Deposited 2009-06-27	Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 54-meric	Chain DV 1–94（94 aa）	Not recorded	MG MAGNESIUM ION × 179 ZN ZINC ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions MICROBATCH；pH 7；291 K；4% PEG 8000, 4% MPD, 3.8 mM MgCl2, 380 mM NH4Cl, 5.5 mM putrescine, 5 mM spermidine, pH 7.0, microbatch, temperature 291K	Resolution 3.81 Å R-free 0.253
4V6E Crystal structure of the E. coli 70S ribosome in an intermediate state of ratcheting Deposited 2009-06-28	Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric	Chain BV 1–94（94 aa）	Not recorded	MG MAGNESIUM ION × 184 ZN ZINC ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions MICROBATCH；pH 7；291 K；4% PEG 8000, 4% MPD, 3.8 mM MgCl2, 380 mM NH4Cl, 5.5 mM putrescine, 5 mM spermidine, pH 7.0, microbatch, temperature 291K	Resolution 3.71 Å R-free 0.268
4V6E Crystal structure of the E. coli 70S ribosome in an intermediate state of ratcheting Deposited 2009-06-28	Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric	Chain DV 1–94（94 aa）	Not recorded	MG MAGNESIUM ION × 179 ZN ZINC ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions MICROBATCH；pH 7；291 K；4% PEG 8000, 4% MPD, 3.8 mM MgCl2, 380 mM NH4Cl, 5.5 mM putrescine, 5 mM spermidine, pH 7.0, microbatch, temperature 291K	Resolution 3.71 Å R-free 0.268
4V6K Structural insights into cognate vs. near-cognate discrimination during decoding. Deposited 2011-01-07	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 58-meric	Chain AW 1–94（94 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen NITROGEN	Resolution 8.25 Å
4V6L Structural insights into cognate vs. near-cognate discrimination during decoding. Deposited 2011-01-07	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 58-meric	Chain BW 1–94（94 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen NITROGEN	Resolution 13.20 Å
4V6M Structure of the ribosome-SecYE complex in the membrane environment Deposited 2011-02-08	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 55 PDB declaration: 60-meric	Chain BV 1–94（94 aa）	Not recorded	PEV (1S)-2-{[(2-AMINOETHOXY)(HYDROXY)PHOSPHORYL]OXY}-1-[(PALMITOYLOXY)METHYL]ETHYL STEARATE × 101 PGV (1R)-2-{[{[(2S)-2,3-DIHYDROXYPROPYL]OXY}(HYDROXY)PHOSPHORYL]OXY}-1-[(PALMITOYLOXY)METHYL]ETHYL (11E)-OCTADEC-11-ENOATE × 32	ELECTRON MICROSCOPY cryo-EM buffer 20 mM Hepes (pH 7.2), 100 mM KOAc, 10 mM Mg(OAc)2, 1 mM DTT, 250 microg/ml chloramphenicol；pH 7.2；20 mM Hepes (pH 7.2), 100 mM KOAc, 10 mM Mg(OAc)2, 1 mM DTT, 250 microg/ml chloramphenicol cryo-EM vitrification conditions Cryogen ETHANE；liquid ethane was used as a cryogen	Resolution 7.10 Å
4V6N Structural characterization of mRNA-tRNA translocation intermediates (50S ribosome of class2 of the six classes) Deposited 2011-12-07	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 58-meric	Chain AX 1–94（94 aa）	Not recorded	FME N-FORMYLMETHIONINE × 1 TRP TRYPTOPHAN × 1	ELECTRON MICROSCOPY cryo-EM buffer HiFi buffer (50 mM Tris-HCl, pH 7.5, 70mM NH4Cl, 30 mM KCl, 3.5 mM MgCl2, 0.5 mM spermidine, 8mM putrescine, 2 mM DTT, 3.5 mM MgCl2)；pH 7.5；HiFi buffer (50 mM Tris-HCl, pH 7.5, 70mM NH4Cl, 30 mM KCl, 3.5 mM MgCl2, 0.5 mM spermidine, 8mM putrescine, 2 mM DTT, 3.5 mM MgCl2) cryo-EM vitrification conditions blot for 3 seconds；80 K；Cryogen NITROGEN；Blot for 3 seconds, plunge into liquid nitrogen (Vitrobot)	Resolution 12.10 Å
4V6O Structural characterization of mRNA-tRNA translocation intermediates (class 4a of the six classes) Deposited 2011-12-07	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 58-meric	Chain BX 1–94（94 aa）	Not recorded	TRP TRYPTOPHAN × 1 FME N-FORMYLMETHIONINE × 1	ELECTRON MICROSCOPY cryo-EM buffer HiFi buffer (50 mM Tris-HCl, pH 7.5, 70mM NH4Cl, 30 mM KCl, 3.5 mM MgCl2, 0.5 mM spermidine, 8mM putrescine, 2 mM DTT, 3.5 mM MgCl2)；pH 7.5；HiFi buffer (50 mM Tris-HCl, pH 7.5, 70mM NH4Cl, 30 mM KCl, 3.5 mM MgCl2, 0.5 mM spermidine, 8mM putrescine, 2 mM DTT, 3.5 mM MgCl2) cryo-EM vitrification conditions blot for 3 seconds；80 K；Cryogen NITROGEN；Blot for 3 seconds, plunge into liquid nitrogen (Vitrobot)	Resolution 14.70 Å
4V6P Structural characterization of mRNA-tRNA translocation intermediates (class 4b of the six classes) Deposited 2011-12-08	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 58-meric	Chain BX 1–94（94 aa）	Not recorded	TRP TRYPTOPHAN × 1 FME N-FORMYLMETHIONINE × 1	ELECTRON MICROSCOPY cryo-EM buffer HiFi buffer (50 mM Tris-HCl, pH 7.5, 70mM NH4Cl, 30 mM KCl, 3.5 mM MgCl2, 0.5 mM spermidine, 8mM putrescine, 2 mM DTT, 3.5 mM MgCl2)；pH 7.5；HiFi buffer (50 mM Tris-HCl, pH 7.5, 70mM NH4Cl, 30 mM KCl, 3.5 mM MgCl2, 0.5 mM spermidine, 8mM putrescine, 2 mM DTT, 3.5 mM MgCl2) cryo-EM vitrification conditions blot for 6 seconds；80 K；Cryogen NITROGEN；Blot for 6 seconds, plunge into liquid nitrogen (Vitrobot)	Resolution 13.50 Å
4V6Q Structural characterization of mRNA-tRNA translocation intermediates (class 5 of the six classes) Deposited 2011-12-08	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 58-meric	Chain BX 1–94（94 aa）	Not recorded	TRP TRYPTOPHAN × 1 FME N-FORMYLMETHIONINE × 1	ELECTRON MICROSCOPY cryo-EM buffer HiFi buffer (50 mM Tris-HCl, pH 7.5, 70mM NH4Cl, 30 mM KCl, 3.5 mM MgCl2, 0.5 mM spermidine, 8mM putrescine, 2 mM DTT, 3.5 mM MgCl2)；pH 7.5；HiFi buffer (50 mM Tris-HCl, pH 7.5, 70mM NH4Cl, 30 mM KCl, 3.5 mM MgCl2, 0.5 mM spermidine, 8mM putrescine, 2 mM DTT, 3.5 mM MgCl2) cryo-EM vitrification conditions blot for 3 seconds；80 K；Cryogen NITROGEN；Blot for 3 seconds, plunge into liquid nitrogen (Vitrobot)	Resolution 11.50 Å
4V6R Structural characterization of mRNA-tRNA translocation intermediates (class 6 of the six classes) Deposited 2011-12-08	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 58-meric	Chain BX 1–94（94 aa）	Not recorded	TRP TRYPTOPHAN × 1 FME N-FORMYLMETHIONINE × 1	ELECTRON MICROSCOPY cryo-EM buffer HiFi buffer (50 mM Tris-HCl, pH 7.5, 70mM NH4Cl, 30 mM KCl, 3.5 mM MgCl2, 0.5 mM spermidine, 8mM putrescine, 2 mM DTT, 3.5 mM MgCl2)；pH 7.5；HiFi buffer (50 mM Tris-HCl, pH 7.5, 70mM NH4Cl, 30 mM KCl, 3.5 mM MgCl2, 0.5 mM spermidine, 8mM putrescine, 2 mM DTT, 3.5 mM MgCl2) cryo-EM vitrification conditions blot for 3 seconds；80 K；Cryogen NITROGEN；Blot for 3 seconds, plunge into liquid nitrogen (Vitrobot)	Resolution 11.50 Å
4V6S Structural characterization of mRNA-tRNA translocation intermediates (class 3 of the six classes) Deposited 2011-12-09	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 57-meric	Chain AX 1–94（94 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer HiFi buffer (50 mM Tris-HCl, pH 7.5, 70mM NH4Cl, 30 mM KCl, 3.5 mM MgCl2, 0.5 mM spermidine, 8mM putrescine, 2 mM DTT, 3.5 mM MgCl2)；pH 7.5；HiFi buffer (50 mM Tris-HCl, pH 7.5, 70mM NH4Cl, 30 mM KCl, 3.5 mM MgCl2, 0.5 mM spermidine, 8mM putrescine, 2 mM DTT, 3.5 mM MgCl2) cryo-EM vitrification conditions blot for 3 seconds；80 K；Cryogen NITROGEN；Blot for 3 seconds, plunge into liquid nitrogen (Vitrobot)	Resolution 13.10 Å
4V6T Structure of the bacterial ribosome complexed by tmRNA-SmpB and EF-G during translocation and MLD-loading Deposited 2012-01-27	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 56-meric	Chain BV 1–94（94 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6 cryo-EM vitrification conditions blot for 5-10 seconds before plunging；96.15 K；Cryogen ETHANE；blot for 5-10 seconds before plunging into liquid ethane (FEI Vitrobot)	Resolution 8.30 Å
4V6V Tetracycline resistance protein Tet(O) bound to the ribosome Deposited 2013-02-25	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 53 PDB declaration: 58-meric	Chain BZ 1–94（94 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM vitrification conditions Cryogen ETHANE	Resolution 9.80 Å
4V6Y E. coli 70S-fMetVal-tRNAVal-tRNAfMet complex in classic pre-translocation state (pre1a) Deposited 2013-10-14	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric	Chain BV 1–94（94 aa）	Not recorded	VAL VALINE × 1 FME N-FORMYLMETHIONINE × 1	ELECTRON MICROSCOPY cryo-EM buffer 50 mM Tris-HCl, 70 mM NH4Cl, 30 mM KCl, 7 mM MgCl2, 0.6 mM spermine, 0.4 mM spermidine；pH 7.5；50 mM Tris-HCl, 70 mM NH4Cl, 30 mM KCl, 7 mM MgCl2, 0.6 mM spermine, 0.4 mM spermidine cryo-EM vitrification conditions Manual blotting for about 2 seconds；Cryogen ETHANE；Manual blotting for about 2 seconds prior to plunging into liquid ethane (custom-built CEVS vitrification instrument with dew point temperature adjusted to 18 degrees C)	Resolution 12.00 Å
4V6Z E. coli 70S-fMetVal-tRNAVal-tRNAfMet complex in classic pre-translocation state (pre1b) Deposited 2013-10-14	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric	Chain BV 1–94（94 aa）	Not recorded	VAL VALINE × 1 FME N-FORMYLMETHIONINE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 12.00 Å
4V70 E. coli 70S-fMetVal-tRNAVal-tRNAfMet complex in intermediate pre-translocation state (pre3) Deposited 2013-10-14	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric	Chain BV 1–94（94 aa）	Not recorded	VAL VALINE × 1 FME N-FORMYLMETHIONINE × 1	ELECTRON MICROSCOPY cryo-EM buffer 50 mM Tris-HCl, 70 mM NH4Cl, 30 mM KCl, 7 mM MgCl2, 0.6 mM spermine, 0.4 mM spermidine；pH 7.5；50 mM Tris-HCl, 70 mM NH4Cl, 30 mM KCl, 7 mM MgCl2, 0.6 mM spermine, 0.4 mM spermidine cryo-EM vitrification conditions Manual blotting for about 2 seconds；Cryogen ETHANE；Manual blotting for about 2 seconds prior to plunging into liquid ethane (custom-built CEVS vitrification instrument with dew point temperature adjusted to 18 degrees C)	Resolution 17.00 Å
4V71 E. coli 70S-fMetVal-tRNAVal-tRNAfMet complex in intermediate pre-translocation state (pre2) Deposited 2013-10-14	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric	Chain BV 1–94（94 aa）	Not recorded	VAL VALINE × 1 FME N-FORMYLMETHIONINE × 1	ELECTRON MICROSCOPY cryo-EM buffer 50 mM Tris-HCl, 70 mM NH4Cl, 30 mM KCl, 7 mM MgCl2, 0.6 mM spermine, 0.4 mM spermidine；pH 7.5；50 mM Tris-HCl, 70 mM NH4Cl, 30 mM KCl, 7 mM MgCl2, 0.6 mM spermine, 0.4 mM spermidine cryo-EM vitrification conditions Manual blotting for about 2 seconds；Cryogen ETHANE；Manual blotting for about 2 seconds prior to plunging into liquid ethane (custom-built CEVS vitrification instrument with dew point temperature adjusted to 18 degrees C)	Resolution 20.00 Å
4V72 E. coli 70S-fMetVal-tRNAVal-tRNAfMet complex in hybrid pre-translocation state (pre4) Deposited 2013-10-14	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric	Chain BV 1–94（94 aa）	Not recorded	VAL VALINE × 1 FME N-FORMYLMETHIONINE × 1	ELECTRON MICROSCOPY cryo-EM buffer 50 mM Tris-HCl, 70 mM NH4Cl, 30 mM KCl, 7 mM MgCl2, 0.6 mM spermine, 0.4 mM spermidine；pH 7.5；50 mM Tris-HCl, 70 mM NH4Cl, 30 mM KCl, 7 mM MgCl2, 0.6 mM spermine, 0.4 mM spermidine cryo-EM vitrification conditions Manual blotting for about 2 seconds；Cryogen ETHANE；Manual blotting for about 2 seconds prior to plunging into liquid ethane (custom-built CEVS vitrification instrument with dew point temperature adjusted to 18 degrees C)	Resolution 13.00 Å
4V73 E. coli 70S-fMetVal-tRNAVal-tRNAfMet complex in hybrid pre-translocation state (pre5a) Deposited 2013-10-14	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric	Chain BV 1–94（94 aa）	Not recorded	VAL VALINE × 1 FME N-FORMYLMETHIONINE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 15.00 Å
4V74 70S-fMetVal-tRNAVal-tRNAfMet complex in hybrid pre-translocation state (pre5b) Deposited 2013-10-14	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric	Chain BV 1–94（94 aa）	Not recorded	VAL VALINE × 1 FME N-FORMYLMETHIONINE × 1	ELECTRON MICROSCOPY cryo-EM buffer 50 mM Tris-HCl, 70 mM NH4Cl, 30 mM KCl, 7 mM MgCl2, 0.6 mM spermine, 0.4 mM spermidine；pH 7.5；50 mM Tris-HCl, 70 mM NH4Cl, 30 mM KCl, 7 mM MgCl2, 0.6 mM spermine, 0.4 mM spermidine cryo-EM vitrification conditions Manual blotting for about 2 seconds；Cryogen ETHANE；Manual blotting for about 2 seconds prior to plunging into liquid ethane (custom-built CEVS vitrification instrument with dew point temperature adjusted to 18 degrees C)	Resolution 17.00 Å
4V75 E. coli 70S-fMetVal-tRNAVal-tRNAfMet complex in classic post-translocation state (post1) Deposited 2013-10-14	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric	Chain BV 1–94（94 aa）	Not recorded	VAL VALINE × 1 FME N-FORMYLMETHIONINE × 1	ELECTRON MICROSCOPY cryo-EM buffer 50 mM Tris-HCl, 70 mM NH4Cl, 30 mM KCl, 7 mM MgCl2, 0.6 mM spermine, 0.4 mM spermidine；pH 7.5；50 mM Tris-HCl, 70 mM NH4Cl, 30 mM KCl, 7 mM MgCl2, 0.6 mM spermine, 0.4 mM spermidine cryo-EM vitrification conditions Manual blotting for about 2 seconds；Cryogen ETHANE；Manual blotting for about 2 seconds prior to plunging into liquid ethane (custom-built CEVS vitrification instrument with dew point temperature adjusted to 18 degrees C)	Resolution 12.00 Å
4V76 E. coli 70S-fMetVal-tRNAVal-tRNAfMet complex in intermediate post-translocation state (post2a) Deposited 2013-10-14	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric	Chain BV 1–94（94 aa）	Not recorded	VAL VALINE × 1 FME N-FORMYLMETHIONINE × 1	ELECTRON MICROSCOPY cryo-EM buffer 50 mM Tris-HCl, 70 mM NH4Cl, 30 mM KCl, 7 mM MgCl2, 0.6 mM spermine, 0.4 mM spermidine；pH 7.5；50 mM Tris-HCl, 70 mM NH4Cl, 30 mM KCl, 7 mM MgCl2, 0.6 mM spermine, 0.4 mM spermidine cryo-EM vitrification conditions Manual blotting for about 2 seconds；Cryogen ETHANE；Manual blotting for about 2 seconds prior to plunging into liquid ethane (custom-built CEVS vitrification instrument with dew point temperature adjusted to 18 degrees C)	Resolution 17.00 Å
4V77 E. coli 70S-fMetVal-tRNAVal-tRNAfMet complex in intermediate post-translocation state (post2b) Deposited 2013-10-14	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric	Chain BV 1–94（94 aa）	Not recorded	VAL VALINE × 1 FME N-FORMYLMETHIONINE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 17.00 Å
4V78 E. coli 70S-fMetVal-tRNAVal-tRNAfMet complex in intermediate post-translocation state (post3a) Deposited 2013-10-14	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric	Chain BV 1–94（94 aa）	Not recorded	VAL VALINE × 1 FME N-FORMYLMETHIONINE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 20.00 Å
4V79 E. coli 70S-fMetVal-tRNAVal-tRNAfMet complex in intermediate post-translocation state (post3b) Deposited 2013-10-14	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric	Chain BV 1–94（94 aa）	Not recorded	VAL VALINE × 1 FME N-FORMYLMETHIONINE × 1	ELECTRON MICROSCOPY cryo-EM buffer 50 mM Tris-HCl, 70 mM NH4Cl, 30 mM KCl, 7 mM MgCl2, 0.6 mM spermine, 0.4 mM spermidine；pH 7.5；50 mM Tris-HCl, 70 mM NH4Cl, 30 mM KCl, 7 mM MgCl2, 0.6 mM spermine, 0.4 mM spermidine cryo-EM vitrification conditions Manual blotting for about 2 seconds；Cryogen ETHANE；Manual blotting for about 2 seconds prior to plunging into liquid ethane (custom-built CEVS vitrification instrument with dew point temperature adjusted to 18 degrees C)	Resolution 15.00 Å
4V7A E. coli 70S-fMetVal-tRNAVal post-translocation complex (post4) Deposited 2013-10-14	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric	Chain BV 1–94（94 aa）	Not recorded	VAL VALINE × 1 FME N-FORMYLMETHIONINE × 1	ELECTRON MICROSCOPY cryo-EM buffer 50 mM Tris-HCl, 70 mM NH4Cl, 30 mM KCl, 7 mM MgCl2, 0.6 mM spermine, 0.4 mM spermidine；pH 7.5；50 mM Tris-HCl, 70 mM NH4Cl, 30 mM KCl, 7 mM MgCl2, 0.6 mM spermine, 0.4 mM spermidine cryo-EM vitrification conditions Manual blotting for about 2 seconds；Cryogen ETHANE；Manual blotting for about 2 seconds prior to plunging into liquid ethane (custom-built CEVS vitrification instrument with dew point temperature adjusted to 18 degrees C)	Resolution 9.00 Å
4V7B Visualization of two tRNAs trapped in transit during EF-G-mediated translocation Deposited 2013-10-27	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 58-meric	Chain BV 1–94（94 aa）	Not recorded	FUA FUSIDIC ACID × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1	ELECTRON MICROSCOPY cryo-EM buffer 80 mM HEPES potassium, 75 mM NH4Cl, 10 mM MgCl2, 6 mM BME；pH 7.6；80 mM HEPES potassium, 75 mM NH4Cl, 10 mM MgCl2, 6 mM BME cryo-EM vitrification conditions blot for 5-10 seconds before plunging；96 K；Cryogen ETHANE；Blot for 5-10 seconds before plunging into liquid ethane (FEI VITROBOT MARK I)	Resolution 6.80 Å
4V7C Structure of the Ribosome with Elongation Factor G Trapped in the Pre-Translocation State (pre-translocation 70S*tRNA structure) Deposited 2013-11-20	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 58-meric	Chain BX 1–94（94 aa）	Not recorded	ZN ZINC ION × 1	ELECTRON MICROSCOPY cryo-EM buffer Polymix buffer；pH 7.6；10 mM HEPES-KOH, 5 mM MgCl2, 90 mM NH4Cl, 2 mM spermidine, 0.1 mM spermine, 6 mM BME, 0.5 mM viomycin, 0.5 mM GTP, 0.5 mM fusidic acid cryo-EM vitrification conditions Freshly glow-disharged grids were loaded into an FEI Mark II Vitrobot and equilibrated to 95% relative humidity at 22 degrees Celsius. 2 microliters of sample was applied through the side port, blotted for 7 seconds with a positional offset of 2, and plunged into liquid ethane.；Cryogen ETHANE；Freshly glow-disharged grids were loaded into an FEI Mark II Vitrobot and equilibrated to 95% relative humidity at 22 degrees Celsius. 2 microliters of sample was applied through the side port, blotted for 7 seconds with a positional offset of 2, and plunged into liquid ethane.	Resolution 7.60 Å
4V7D Structure of the Ribosome with Elongation Factor G Trapped in the Pre-Translocation State (pre-translocation 70StRNAEF-G structure) Deposited 2013-11-21	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 54 PDB declaration: 60-meric	Chain AY 1–94（94 aa）	Not recorded	ZN ZINC ION × 1	ELECTRON MICROSCOPY cryo-EM buffer Polymix buffer；pH 7.6；10 mM HEPES-KOH, 5 mM MgCl2, 90 mM NH4Cl, 2 mM spermidine, 0.1 mM spermine, 6 mM BME, 0.5 mM viomycin, 0.5 mM GTP, 0.5 mM fusidic acid cryo-EM vitrification conditions Freshly glow-disharged grids were loaded into an FEI Mark II Vitrobot and equilibrated to 95% relative humidity at 22 degrees Celsius. 2 microliters of sample was applied through the side port, blotted for 7 seconds with a positional offset of 2, and plunged into liquid ethane.；Cryogen ETHANE；Freshly glow-disharged grids were loaded into an FEI Mark II Vitrobot and equilibrated to 95% relative humidity at 22 degrees Celsius. 2 microliters of sample was applied through the side port, blotted for 7 seconds with a positional offset of 2, and plunged into liquid ethane.	Resolution 7.60 Å
4V7I Ribosome-SecY complex. Deposited 2009-10-21	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 53 PDB declaration: 56-meric	Chain AV 1–94（94 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer 50 MM HEPES- KOH PH 7.5, 100 MM KOAC, 10 MM MG(OAC)2, 0.05% DDM；pH 7.5；50 MM HEPES- KOH PH 7.5, 100 MM KOAC, 10 MM MG(OAC)2, 0.05% DDM cryo-EM vitrification conditions Cryogen ETHANE；THE SPECIMENS WERE PLUNGE FROZEN IN LIQUID ETHANE AT 4 DEGREES C AT AN RH OF ~90-95%.	Resolution 9.60 Å
4V7S Crystal structure of the E. coli ribosome bound to telithromycin. Deposited 2010-08-05	Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 52-meric	Chain BV 1–94（94 aa）	Not recorded	MG MAGNESIUM ION × 181 TEL TELITHROMYCIN × 1 ZN ZINC ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions MICROBATCH；pH 6.5；291 K；PEG8k, MPD, pH 6.5, microbatch, temperature 291K	Resolution 3.25 Å R-free 0.245
4V7S Crystal structure of the E. coli ribosome bound to telithromycin. Deposited 2010-08-05	Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 52-meric	Chain DV 1–94（94 aa）	Not recorded	MG MAGNESIUM ION × 179 ZN ZINC ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions MICROBATCH；pH 6.5；291 K；PEG8k, MPD, pH 6.5, microbatch, temperature 291K	Resolution 3.25 Å R-free 0.245
4V7T Crystal structure of the E. coli ribosome bound to chloramphenicol. Deposited 2010-08-14	Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 52-meric	Chain BV 1–94（94 aa）	Not recorded	MG MAGNESIUM ION × 183 CLM CHLORAMPHENICOL × 1 ZN ZINC ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions MICROBATCH；pH 6.5；291 K；PEG8k, MPD, pH 6.5, microbatch, temperature 291K	Resolution 3.19 Å R-free 0.252
4V7T Crystal structure of the E. coli ribosome bound to chloramphenicol. Deposited 2010-08-14	Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 52-meric	Chain DV 1–94（94 aa）	Not recorded	MG MAGNESIUM ION × 179 ZN ZINC ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions MICROBATCH；pH 6.5；291 K；PEG8k, MPD, pH 6.5, microbatch, temperature 291K	Resolution 3.19 Å R-free 0.252
4V7U Crystal structure of the E. coli ribosome bound to erythromycin. Deposited 2010-08-15	Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 52-meric	Chain BV 1–94（94 aa）	Not recorded	MG MAGNESIUM ION × 182 ERY ERYTHROMYCIN A × 1 ZN ZINC ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions MICROBATCH；pH 6.5；291 K；4.3% PEG 8000, 4.9% MPD, 3.8 mM MgCl2, 380 mM NH4Cl, 5.5 mM putrescine, 5 mM spermidine, 10 mM Tris, 20 mM MES pH 6.5. 0.25 mM EDTA, microbatch, temperature 291K	Resolution 3.10 Å R-free 0.254
4V7U Crystal structure of the E. coli ribosome bound to erythromycin. Deposited 2010-08-15	Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 52-meric	Chain DV 1–94（94 aa）	Not recorded	MG MAGNESIUM ION × 179 ZN ZINC ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions MICROBATCH；pH 6.5；291 K；4.3% PEG 8000, 4.9% MPD, 3.8 mM MgCl2, 380 mM NH4Cl, 5.5 mM putrescine, 5 mM spermidine, 10 mM Tris, 20 mM MES pH 6.5. 0.25 mM EDTA, microbatch, temperature 291K	Resolution 3.10 Å R-free 0.254
4V7V Crystal structure of the E. coli ribosome bound to clindamycin. Deposited 2010-08-16	Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 52-meric	Chain BV 1–94（94 aa）	Not recorded	MG MAGNESIUM ION × 182 CLY CLINDAMYCIN × 1 ZN ZINC ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions MICROBATCH；291 K；4.3% PEG 8000, 4.9% MPD, 10 mM Tris, 20 mM MES, pH 6.5-7.0, 3.8 mM MgCl2, 380 mM NH4Cl, 5.5 mM putrescine, 5 mM spermidine, 0.25 mM EDTA, microbatch, temperature 291K	Resolution 3.29 Å R-free 0.241
4V7V Crystal structure of the E. coli ribosome bound to clindamycin. Deposited 2010-08-16	Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 52-meric	Chain DV 1–94（94 aa）	Not recorded	MG MAGNESIUM ION × 179 ZN ZINC ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions MICROBATCH；291 K；4.3% PEG 8000, 4.9% MPD, 10 mM Tris, 20 mM MES, pH 6.5-7.0, 3.8 mM MgCl2, 380 mM NH4Cl, 5.5 mM putrescine, 5 mM spermidine, 0.25 mM EDTA, microbatch, temperature 291K	Resolution 3.29 Å R-free 0.241
4V89 Crystal Structure of Release Factor RF3 Trapped in the GTP State on a Rotated Conformation of the Ribosome (without viomycin) Deposited 2011-11-17	Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 54 PDB declaration: 58-meric	Chain BZ 1–94（94 aa）	Not recorded	GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；295 K；Tris Ac PH.7.0 25-35 mM KCL 6.1% PEG 20000 %1 glycerol 50mM sucrose , VAPOR DIFFUSION, SITTING DROP, temperature 295K	Resolution 3.70 Å R-free 0.290
4V9C Allosteric control of the ribosome by small-molecule antibiotics Deposited 2012-07-25	Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 54-meric	Chain BV 1–94（94 aa）	Not recorded	MG MAGNESIUM ION × 224 NMY NEOMYCIN × 10 ZN ZINC ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions pH 6.5；291 K；PEG800, MPD, pH 6.5, microbatch, temperature 291K	Resolution 3.30 Å R-free 0.255
4V9C Allosteric control of the ribosome by small-molecule antibiotics Deposited 2012-07-25	Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric	Chain DV 1–94（94 aa）	Not recorded	MG MAGNESIUM ION × 266 NMY NEOMYCIN × 8 ZN ZINC ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions pH 6.5；291 K；PEG800, MPD, pH 6.5, microbatch, temperature 291K	Resolution 3.30 Å R-free 0.255
4V9D Structures of the bacterial ribosome in classical and hybrid states of tRNA binding Deposited 2012-07-31	Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric	Chain CV 1–94（94 aa）	Not recorded	MG MAGNESIUM ION × 271 ZN ZINC ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions MICROBATCH；pH 6.5；291 K；PEG8000, MPD, pH 6.5, microbatch, temperature 291K X-ray crystallization conditions MICROBATCH；pH 6.5；291 K；PEG8000, MPD, pH 6.5, microbatch, temperature 291K X-ray crystallization conditions MICROBATCH；pH 6.5；291 K；PEG8000, MPD, pH 6.5, microbatch, temperature 291K X-ray crystallization conditions MICROBATCH；pH 6.5；291 K；PEG8000, MPD, pH 6.5, microbatch, temperature 291K X-ray crystallization conditions MICROBATCH；pH 6.5；291 K；PEG8000, MPD, pH 6.5, microbatch, temperature 291K	Resolution 3.00 Å R-free 0.260
4V9D Structures of the bacterial ribosome in classical and hybrid states of tRNA binding Deposited 2012-07-31	Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 54-meric	Chain DV 1–94（94 aa）	Not recorded	MG MAGNESIUM ION × 227 ZN ZINC ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions MICROBATCH；pH 6.5；291 K；PEG8000, MPD, pH 6.5, microbatch, temperature 291K X-ray crystallization conditions MICROBATCH；pH 6.5；291 K；PEG8000, MPD, pH 6.5, microbatch, temperature 291K X-ray crystallization conditions MICROBATCH；pH 6.5；291 K；PEG8000, MPD, pH 6.5, microbatch, temperature 291K X-ray crystallization conditions MICROBATCH；pH 6.5；291 K；PEG8000, MPD, pH 6.5, microbatch, temperature 291K X-ray crystallization conditions MICROBATCH；pH 6.5；291 K；PEG8000, MPD, pH 6.5, microbatch, temperature 291K	Resolution 3.00 Å R-free 0.260
4V9O Control of ribosomal subunit rotation by elongation factor G Deposited 2013-05-03	Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 53 PDB declaration: 56-meric	Chain AV 1–94（94 aa）	Not recorded	MG MAGNESIUM ION × 187 ZN ZINC ION × 1 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1	X-RAY DIFFRACTION X-ray crystallization conditions MICROBATCH；pH 6.5；291 K；PEG8k, MPD, KSCN, pH 6.5, microbatch, temperature 291K X-ray crystallization conditions MICROBATCH；pH 6.5；291 K；PEG8k, MPD, KSCN, pH 6.5, microbatch, temperature 291K X-ray crystallization conditions MICROBATCH；pH 6.5；291 K；PEG8k, MPD, KSCN, pH 6.5, microbatch, temperature 291K X-ray crystallization conditions MICROBATCH；pH 6.5；291 K；PEG8k, KSCN, pH 6.5, microbatch, temperature 291K X-ray crystallization conditions MICROBATCH；pH 6.5；291 K；PEG8k, KSCN, pH 6.5, microbatch, temperature 291K	Resolution 2.90 Å R-free 0.272
4V9O Control of ribosomal subunit rotation by elongation factor G Deposited 2013-05-03	Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 2 Protein–RNA Heteromer；Protein × 52 PDB declaration: 55-meric	Chain CV 1–94（94 aa）	Not recorded	MG MAGNESIUM ION × 187 ZN ZINC ION × 1 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1	X-RAY DIFFRACTION X-ray crystallization conditions MICROBATCH；pH 6.5；291 K；PEG8k, MPD, KSCN, pH 6.5, microbatch, temperature 291K X-ray crystallization conditions MICROBATCH；pH 6.5；291 K；PEG8k, MPD, KSCN, pH 6.5, microbatch, temperature 291K X-ray crystallization conditions MICROBATCH；pH 6.5；291 K；PEG8k, MPD, KSCN, pH 6.5, microbatch, temperature 291K X-ray crystallization conditions MICROBATCH；pH 6.5；291 K；PEG8k, KSCN, pH 6.5, microbatch, temperature 291K X-ray crystallization conditions MICROBATCH；pH 6.5；291 K；PEG8k, KSCN, pH 6.5, microbatch, temperature 291K	Resolution 2.90 Å R-free 0.272
4V9O Control of ribosomal subunit rotation by elongation factor G Deposited 2013-05-03	Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 3 Protein–RNA Heteromer；Protein × 52 PDB declaration: 55-meric	Chain EV 1–94（94 aa）	Not recorded	MG MAGNESIUM ION × 187 ZN ZINC ION × 1 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1	X-RAY DIFFRACTION X-ray crystallization conditions MICROBATCH；pH 6.5；291 K；PEG8k, MPD, KSCN, pH 6.5, microbatch, temperature 291K X-ray crystallization conditions MICROBATCH；pH 6.5；291 K；PEG8k, MPD, KSCN, pH 6.5, microbatch, temperature 291K X-ray crystallization conditions MICROBATCH；pH 6.5；291 K；PEG8k, MPD, KSCN, pH 6.5, microbatch, temperature 291K X-ray crystallization conditions MICROBATCH；pH 6.5；291 K；PEG8k, KSCN, pH 6.5, microbatch, temperature 291K X-ray crystallization conditions MICROBATCH；pH 6.5；291 K；PEG8k, KSCN, pH 6.5, microbatch, temperature 291K	Resolution 2.90 Å R-free 0.272
4V9O Control of ribosomal subunit rotation by elongation factor G Deposited 2013-05-03	Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 4 Protein–RNA Heteromer；Protein × 51 PDB declaration: 54-meric	Chain GV 1–94（94 aa）	Not recorded	MG MAGNESIUM ION × 187 ZN ZINC ION × 1 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1	X-RAY DIFFRACTION X-ray crystallization conditions MICROBATCH；pH 6.5；291 K；PEG8k, MPD, KSCN, pH 6.5, microbatch, temperature 291K X-ray crystallization conditions MICROBATCH；pH 6.5；291 K；PEG8k, MPD, KSCN, pH 6.5, microbatch, temperature 291K X-ray crystallization conditions MICROBATCH；pH 6.5；291 K；PEG8k, MPD, KSCN, pH 6.5, microbatch, temperature 291K X-ray crystallization conditions MICROBATCH；pH 6.5；291 K；PEG8k, KSCN, pH 6.5, microbatch, temperature 291K X-ray crystallization conditions MICROBATCH；pH 6.5；291 K；PEG8k, KSCN, pH 6.5, microbatch, temperature 291K	Resolution 2.90 Å R-free 0.272
4V9P Control of ribosomal subunit rotation by elongation factor G Deposited 2013-05-03	Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 55-meric	Chain AV 1–94（94 aa）	Not recorded	MG MAGNESIUM ION × 185 ZN ZINC ION × 1 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1	X-RAY DIFFRACTION X-ray crystallization conditions MICROBATCH；pH 6.5；291 K；PEG8k, MPD, KSCN, pH 6.5, microbatch, temperature 291K X-ray crystallization conditions MICROBATCH；pH 6.5；291 K；PEG8k, MPD, KSCN, pH 6.5, microbatch, temperature 291K X-ray crystallization conditions MICROBATCH；pH 6.5；291 K；PEG8k, MPD, KSCN, pH 6.5, microbatch, temperature 291K X-ray crystallization conditions MICROBATCH；pH 6.5；291 K；PEG8k, KSCN, pH 6.5, microbatch, temperature 291K X-ray crystallization conditions MICROBATCH；pH 6.5；291 K；PEG8k, KSCN, pH 6.5, microbatch, temperature 291K	Resolution 2.90 Å R-free 0.267
4V9P Control of ribosomal subunit rotation by elongation factor G Deposited 2013-05-03	Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 2 Protein–RNA Heteromer；Protein × 51 PDB declaration: 54-meric	Chain CV 1–94（94 aa）	Not recorded	MG MAGNESIUM ION × 185 ZN ZINC ION × 1 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1	X-RAY DIFFRACTION X-ray crystallization conditions MICROBATCH；pH 6.5；291 K；PEG8k, MPD, KSCN, pH 6.5, microbatch, temperature 291K X-ray crystallization conditions MICROBATCH；pH 6.5；291 K；PEG8k, MPD, KSCN, pH 6.5, microbatch, temperature 291K X-ray crystallization conditions MICROBATCH；pH 6.5；291 K；PEG8k, MPD, KSCN, pH 6.5, microbatch, temperature 291K X-ray crystallization conditions MICROBATCH；pH 6.5；291 K；PEG8k, KSCN, pH 6.5, microbatch, temperature 291K X-ray crystallization conditions MICROBATCH；pH 6.5；291 K；PEG8k, KSCN, pH 6.5, microbatch, temperature 291K	Resolution 2.90 Å R-free 0.267
4V9P Control of ribosomal subunit rotation by elongation factor G Deposited 2013-05-03	Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 3 Protein–RNA Heteromer；Protein × 52 PDB declaration: 55-meric	Chain EV 1–94（94 aa）	Not recorded	MG MAGNESIUM ION × 185 ZN ZINC ION × 1 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1	X-RAY DIFFRACTION X-ray crystallization conditions MICROBATCH；pH 6.5；291 K；PEG8k, MPD, KSCN, pH 6.5, microbatch, temperature 291K X-ray crystallization conditions MICROBATCH；pH 6.5；291 K；PEG8k, MPD, KSCN, pH 6.5, microbatch, temperature 291K X-ray crystallization conditions MICROBATCH；pH 6.5；291 K；PEG8k, MPD, KSCN, pH 6.5, microbatch, temperature 291K X-ray crystallization conditions MICROBATCH；pH 6.5；291 K；PEG8k, KSCN, pH 6.5, microbatch, temperature 291K X-ray crystallization conditions MICROBATCH；pH 6.5；291 K；PEG8k, KSCN, pH 6.5, microbatch, temperature 291K	Resolution 2.90 Å R-free 0.267
4V9P Control of ribosomal subunit rotation by elongation factor G Deposited 2013-05-03	Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 4 Protein–RNA Heteromer；Protein × 50 PDB declaration: 53-meric	Chain GV 1–94（94 aa）	Not recorded	MG MAGNESIUM ION × 185 ZN ZINC ION × 1 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1	X-RAY DIFFRACTION X-ray crystallization conditions MICROBATCH；pH 6.5；291 K；PEG8k, MPD, KSCN, pH 6.5, microbatch, temperature 291K X-ray crystallization conditions MICROBATCH；pH 6.5；291 K；PEG8k, MPD, KSCN, pH 6.5, microbatch, temperature 291K X-ray crystallization conditions MICROBATCH；pH 6.5；291 K；PEG8k, MPD, KSCN, pH 6.5, microbatch, temperature 291K X-ray crystallization conditions MICROBATCH；pH 6.5；291 K；PEG8k, KSCN, pH 6.5, microbatch, temperature 291K X-ray crystallization conditions MICROBATCH；pH 6.5；291 K；PEG8k, KSCN, pH 6.5, microbatch, temperature 291K	Resolution 2.90 Å R-free 0.267
4WF1 Crystal structure of the E. coli ribosome bound to negamycin. Deposited 2014-09-11	Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 53-meric	Chain BV 1–94（94 aa）	Not recorded	MG MAGNESIUM ION × 271 ZN ZINC ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions MICROBATCH；pH 6.5；291 K；PEG8k, MPD	Resolution 3.09 Å R-free 0.244
4WF1 Crystal structure of the E. coli ribosome bound to negamycin. Deposited 2014-09-11	Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 52-meric	Chain DV 1–94（94 aa）	Not recorded	MG MAGNESIUM ION × 228 ZN ZINC ION × 1 NEG NEGAMYCIN × 1	X-RAY DIFFRACTION X-ray crystallization conditions MICROBATCH；pH 6.5；291 K；PEG8k, MPD	Resolution 3.09 Å R-free 0.244
4WOI 4,5-linked aminoglycoside antibiotics regulate the bacterial ribosome by targeting dynamic conformational processes within intersubunit bridge B2 Deposited 2014-10-15	Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 56-meric	Chain BV 1–94（94 aa）	Not recorded	MG MAGNESIUM ION × 269 PAR PAROMOMYCIN × 6 ZN ZINC ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions MICROBATCH；pH 6.5；291 K；PEG8k, MPD, KCl, KSCN	Resolution 3.00 Å R-free 0.253
4WOI 4,5-linked aminoglycoside antibiotics regulate the bacterial ribosome by targeting dynamic conformational processes within intersubunit bridge B2 Deposited 2014-10-15	Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 54-meric	Chain CV 1–94（94 aa）	Not recorded	MG MAGNESIUM ION × 226 PAR PAROMOMYCIN × 7 ZN ZINC ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions MICROBATCH；pH 6.5；291 K；PEG8k, MPD, KCl, KSCN	Resolution 3.00 Å R-free 0.253
4WWW Crystal structure of the E. coli ribosome bound to CEM-101 Deposited 2014-11-12	Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 52-meric	Chain RV 1–94（94 aa）	Not recorded	MG MAGNESIUM ION × 182 EM1 (3aS,4R,7S,9R,10R,11R,13R,15R,15aR)-1-{4-[4-(3-aminophenyl)-1H-1,2,3-triazol-1-yl]butyl}-4-ethyl-7-fluoro-11-methoxy-3a ,7,9,11,13,15-hexamethyl-2,6,8,14-tetraoxotetradecahydro-2H-oxacyclotetradecino[4,3-d][1,3]oxazol-10-yl 3,4,6-trideoxy-3-(dimethylamino)-beta-D-xylo-hexopyranoside × 1 ZN ZINC ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions MICROBATCH；pH 6.5；291 K；PEG8k, MPD	Resolution 3.10 Å R-free 0.252
4WWW Crystal structure of the E. coli ribosome bound to CEM-101 Deposited 2014-11-12	Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 52-meric	Chain YV 1–94（94 aa）	Not recorded	MG MAGNESIUM ION × 179 ZN ZINC ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions MICROBATCH；pH 6.5；291 K；PEG8k, MPD	Resolution 3.10 Å R-free 0.252
4YBB High-resolution structure of the Escherichia coli ribosome Deposited 2015-02-18	Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 53-meric	Chain DW 1–94（94 aa）	Not recorded	MG MAGNESIUM ION × 263 PG4 TETRAETHYLENE GLYCOL × 6 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 14 PUT 1,4-DIAMINOBUTANE × 16 ZN ZINC ION × 2 PEG DI(HYDROXYETHYL)ETHER × 11 SPD SPERMIDINE × 4 1PE PENTAETHYLENE GLYCOL × 2 PGE TRIETHYLENE GLYCOL × 9 ACY ACETIC ACID × 3 EDO 1,2-ETHANEDIOL × 12 GUN GUANINE × 1 TRS 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL × 1	X-RAY DIFFRACTION X-ray crystallization conditions MICROBATCH；pH 6.5；291 K；PEG8k, MPD	Resolution 2.10 Å R-free 0.234
4YBB High-resolution structure of the Escherichia coli ribosome Deposited 2015-02-18	Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 52-meric	Chain CW 1–94（94 aa）	Not recorded	MG MAGNESIUM ION × 200 PG4 TETRAETHYLENE GLYCOL × 1 ZN ZINC ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions MICROBATCH；pH 6.5；291 K；PEG8k, MPD	Resolution 2.10 Å R-free 0.234
5ADY Cryo-EM structures of the 50S ribosome subunit bound with HflX Deposited 2015-08-25	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 32 PDB declaration: 34-meric	Chain V 1–94（94 aa）	Not recorded	GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1	ELECTRON MICROSCOPY cryo-EM buffer 20MM TRIS-HCL, 100MM NH4CL, 10MM MGCL2；pH 7.5；20MM TRIS-HCL, 100MM NH4CL, 10MM MGCL2 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 4.50 Å
5AFI 2.9A Structure of E. coli ribosome-EF-TU complex by cs-corrected cryo-EM Deposited 2015-01-22	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 59-meric	Chain V 1–94（94 aa）	Not recorded	MG MAGNESIUM ION × 333 CL CHLORIDE ION × 2 FME N-FORMYLMETHIONINE × 1 KIR KIRROMYCIN × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 NA SODIUM ION × 2 ZN ZINC ION × 2	ELECTRON MICROSCOPY cryo-EM buffer 50MM HEPES-KOH, 70MMNH4CL, 30MM KCL, 20MM MGCL2, 1MM DTT, 0.6MM SPERMINE, 0.4MM SPERMIDINE, 0.15MM KIRROMYCIN；pH 7.5；50MM HEPES-KOH, 70MMNH4CL, 30MM KCL, 20MM MGCL2, 1MM DTT, 0.6MM SPERMINE, 0.4MM SPERMIDINE, 0.15MM KIRROMYCIN cryo-EM vitrification conditions Cryogen ETHANE；LIQUIDE ETHANE	Resolution 2.90 Å
5AKA EM structure of ribosome-SRP-FtsY complex in closed state Deposited 2015-03-03	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 31 PDB declaration: 34-meric	Chain V 1–94（94 aa）	Not recorded	MG MAGNESIUM ION × 110	ELECTRON MICROSCOPY cryo-EM buffer 50MM HEPES-KOH, 100MM KOAC, 8MM MG(OAC)2, 500UG/ML CHLORAMPHENICO；pH 7.5；50MM HEPES-KOH, 100MM KOAC, 8MM MG(OAC)2, 500UG/ML CHLORAMPHENICO cryo-EM vitrification conditions Cryogen ETHANE；LIQUID ETHAN	Resolution 5.70 Å
5GAD RNC-SRP-SR complex early state Deposited 2015-11-24	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 33 PDB declaration: 37-meric	Chain W 1–94（94 aa）	Not recorded	MG MAGNESIUM ION × 433 ZN ZINC ION × 1 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.70 Å
5GAE RNC in complex with a translocating SecYEG Deposited 2015-11-25	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 33 PDB declaration: 36-meric	Chain W 1–94（94 aa）	Not recorded	MG MAGNESIUM ION × 323 ZN ZINC ION × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.33 Å
5GAF RNC in complex with SRP Deposited 2015-11-25	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 32 PDB declaration: 36-meric	Chain W 1–94（94 aa）	Not recorded	MG MAGNESIUM ION × 431 ZN ZINC ION × 1 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 4.30 Å
5GAG RNC in complex with SRP-SR in the closed state Deposited 2015-11-26	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 32 PDB declaration: 36-meric	Chain W 1–94（94 aa）	Not recorded	MG MAGNESIUM ION × 431 ZN ZINC ION × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.80 Å
5GAH RNC in complex with SRP with detached NG domain Deposited 2015-11-26	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 32 PDB declaration: 36-meric	Chain W 1–94（94 aa）	Not recorded	MG MAGNESIUM ION × 431 ZN ZINC ION × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.80 Å
5H5U Mechanistic insights into the alternative translation termination by ArfA and RF2 Deposited 2016-11-09	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 57-meric	Chain W 1–94（94 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.01 Å
5IQR Structure of RelA bound to the 70S ribosome Deposited 2016-03-11	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 59-meric	Chain V 1–94（94 aa）	Not recorded	MG MAGNESIUM ION × 299 ZN ZINC ION × 3 PAR PAROMOMYCIN × 1 MET METHIONINE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE；Grids were blotted for 5 s	Resolution 3.00 Å
5IT8 High-resolution structure of the Escherichia coli ribosome Deposited 2016-03-16	Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 53-meric	Chain DW 1–94（94 aa）	Not recorded	MG MAGNESIUM ION × 266 PG4 TETRAETHYLENE GLYCOL × 6 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 14 PUT 1,4-DIAMINOBUTANE × 16 ZN ZINC ION × 2 PEG DI(HYDROXYETHYL)ETHER × 11 EDO 1,2-ETHANEDIOL × 12 PGE TRIETHYLENE GLYCOL × 9 SPD SPERMIDINE × 4 1PE PENTAETHYLENE GLYCOL × 2 ACY ACETIC ACID × 3 GUN GUANINE × 1 TRS 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL × 1	X-RAY DIFFRACTION X-ray crystallization conditions MICROBATCH；pH 6.5；291 K；PEG8k, MPD	Resolution 3.12 Å R-free 0.220
5IT8 High-resolution structure of the Escherichia coli ribosome Deposited 2016-03-16	Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 52-meric	Chain CW 1–94（94 aa）	Not recorded	MG MAGNESIUM ION × 200 PG4 TETRAETHYLENE GLYCOL × 1 ZN ZINC ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions MICROBATCH；pH 6.5；291 K；PEG8k, MPD	Resolution 3.12 Å R-free 0.220
5J5B Structure of the WT E coli ribosome bound to tetracycline Deposited 2016-04-01	Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 53-meric	Chain DW 1–94（94 aa）	Not recorded	MG MAGNESIUM ION × 267 PG4 TETRAETHYLENE GLYCOL × 6 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 14 PUT 1,4-DIAMINOBUTANE × 16 TAC TETRACYCLINE × 1 ZN ZINC ION × 2 PEG DI(HYDROXYETHYL)ETHER × 11 EDO 1,2-ETHANEDIOL × 12 PGE TRIETHYLENE GLYCOL × 9 SPD SPERMIDINE × 4 1PE PENTAETHYLENE GLYCOL × 2 ACY ACETIC ACID × 3 GUN GUANINE × 1 TRS 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL × 1	X-RAY DIFFRACTION X-ray crystallization conditions MICROBATCH；291 K；PEG 8000, Spermidine, Putrescine, Ammonium Chloride, Magnesium Chloride, MES pH 6.5	Resolution 2.80 Å R-free 0.219
5J5B Structure of the WT E coli ribosome bound to tetracycline Deposited 2016-04-01	Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 52-meric	Chain CW 1–94（94 aa）	Not recorded	MG MAGNESIUM ION × 202 PG4 TETRAETHYLENE GLYCOL × 1 TAC TETRACYCLINE × 1 ZN ZINC ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions MICROBATCH；291 K；PEG 8000, Spermidine, Putrescine, Ammonium Chloride, Magnesium Chloride, MES pH 6.5	Resolution 2.80 Å R-free 0.219
5J7L Structure of the 70S E coli ribosome with the U1052G mutation in the 16S rRNA bound to tetracycline Deposited 2016-04-06	Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 53-meric	Chain DW 1–94（94 aa）	Not recorded	MG MAGNESIUM ION × 268 PG4 TETRAETHYLENE GLYCOL × 6 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 14 PUT 1,4-DIAMINOBUTANE × 16 TAC TETRACYCLINE × 2 ZN ZINC ION × 2 PEG DI(HYDROXYETHYL)ETHER × 11 EDO 1,2-ETHANEDIOL × 12 PGE TRIETHYLENE GLYCOL × 9 SPD SPERMIDINE × 4 1PE PENTAETHYLENE GLYCOL × 2 ACY ACETIC ACID × 3 GUN GUANINE × 1 TRS 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL × 1	X-RAY DIFFRACTION X-ray crystallization conditions MICROBATCH；291 K；PEG 8k, Spermidine, Putrescine, Ammonium Chloride, Magnesium Chloride, MES pH 6.5	Resolution 3.00 Å R-free 0.193
5J7L Structure of the 70S E coli ribosome with the U1052G mutation in the 16S rRNA bound to tetracycline Deposited 2016-04-06	Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 52-meric	Chain CW 1–94（94 aa）	Not recorded	MG MAGNESIUM ION × 204 PG4 TETRAETHYLENE GLYCOL × 1 TAC TETRACYCLINE × 2 ZN ZINC ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions MICROBATCH；291 K；PEG 8k, Spermidine, Putrescine, Ammonium Chloride, Magnesium Chloride, MES pH 6.5	Resolution 3.00 Å R-free 0.193
5J88 Structure of the E coli 70S ribosome with the U1060A mutation in 16S rRNA Deposited 2016-04-07	Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 53-meric	Chain DW 1–94（94 aa）	Not recorded	MG MAGNESIUM ION × 266 PG4 TETRAETHYLENE GLYCOL × 6 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 14 PUT 1,4-DIAMINOBUTANE × 16 ZN ZINC ION × 2 PEG DI(HYDROXYETHYL)ETHER × 11 EDO 1,2-ETHANEDIOL × 12 PGE TRIETHYLENE GLYCOL × 9 SPD SPERMIDINE × 4 1PE PENTAETHYLENE GLYCOL × 2 ACY ACETIC ACID × 3 GUN GUANINE × 1 TRS 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL × 1	X-RAY DIFFRACTION X-ray crystallization conditions MICROBATCH；291 K；PEG 8k, Spermidine, Putrescine, Ammonium Chloride, Magnesium Chloride, MES pH 6.5	Resolution 3.32 Å R-free 0.219
5J88 Structure of the E coli 70S ribosome with the U1060A mutation in 16S rRNA Deposited 2016-04-07	Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 52-meric	Chain CW 1–94（94 aa）	Not recorded	MG MAGNESIUM ION × 200 PG4 TETRAETHYLENE GLYCOL × 1 ZN ZINC ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions MICROBATCH；291 K；PEG 8k, Spermidine, Putrescine, Ammonium Chloride, Magnesium Chloride, MES pH 6.5	Resolution 3.32 Å R-free 0.219
5J91 Structure of the Wild-type 70S E coli ribosome bound to Tigecycline Deposited 2016-04-08	Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 53-meric	Chain DW 1–94（94 aa）	Not recorded	MG MAGNESIUM ION × 267 PG4 TETRAETHYLENE GLYCOL × 6 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 14 PUT 1,4-DIAMINOBUTANE × 16 T1C TIGECYCLINE × 1 ZN ZINC ION × 2 PEG DI(HYDROXYETHYL)ETHER × 11 EDO 1,2-ETHANEDIOL × 12 PGE TRIETHYLENE GLYCOL × 9 SPD SPERMIDINE × 4 1PE PENTAETHYLENE GLYCOL × 2 ACY ACETIC ACID × 3 GUN GUANINE × 1 TRS 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL × 1	X-RAY DIFFRACTION X-ray crystallization conditions MICROBATCH；pH 6.5；291 K；PEG 8k, Spermidine, Putrescine, Ammonium Chloride, MAgnesium Chloride, MPD, MES pH 6.5	Resolution 2.96 Å R-free 0.221
5J91 Structure of the Wild-type 70S E coli ribosome bound to Tigecycline Deposited 2016-04-08	Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 52-meric	Chain CW 1–94（94 aa）	Not recorded	MG MAGNESIUM ION × 202 PG4 TETRAETHYLENE GLYCOL × 1 T1C TIGECYCLINE × 1 ZN ZINC ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions MICROBATCH；pH 6.5；291 K；PEG 8k, Spermidine, Putrescine, Ammonium Chloride, MAgnesium Chloride, MPD, MES pH 6.5	Resolution 2.96 Å R-free 0.221
5JC9 Structure of the Escherichia coli ribosome with the U1052G mutation in the 16S rRNA Deposited 2016-04-14	Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 53-meric	Chain DW 1–94（94 aa）	Not recorded	MG MAGNESIUM ION × 267 PG4 TETRAETHYLENE GLYCOL × 6 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 14 PUT 1,4-DIAMINOBUTANE × 16 ZN ZINC ION × 2 PEG DI(HYDROXYETHYL)ETHER × 11 EDO 1,2-ETHANEDIOL × 12 PGE TRIETHYLENE GLYCOL × 9 SPD SPERMIDINE × 4 1PE PENTAETHYLENE GLYCOL × 2 ACY ACETIC ACID × 3 GUN GUANINE × 1 TRS 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL × 1	X-RAY DIFFRACTION X-ray crystallization conditions MICROBATCH；pH 6.5；291 K；PEG 8k, Spermidine, Putrescine, Ammonium Chloride, MAgnesium Chloride, MPD, MES	Resolution 3.03 Å R-free 0.200
5JC9 Structure of the Escherichia coli ribosome with the U1052G mutation in the 16S rRNA Deposited 2016-04-14	Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 52-meric	Chain CW 1–94（94 aa）	Not recorded	MG MAGNESIUM ION × 202 PG4 TETRAETHYLENE GLYCOL × 1 ZN ZINC ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions MICROBATCH；pH 6.5；291 K；PEG 8k, Spermidine, Putrescine, Ammonium Chloride, MAgnesium Chloride, MPD, MES	Resolution 3.03 Å R-free 0.200
5JTE Cryo-EM structure of an ErmBL-stalled ribosome in complex with A-, P-, and E-tRNA Deposited 2016-05-09	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 58-meric	Chain BV 1–94（94 aa）	Not recorded	LYS LYSINE × 1 ERY ERYTHROMYCIN A × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.60 Å
5JU8 Cryo-EM structure of an ErmBL-stalled ribosome in complex with P-, and E-tRNA Deposited 2016-05-10	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric	Chain BV 1–94（94 aa）	Not recorded	ERY ERYTHROMYCIN A × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.60 Å
5KCR Cryo-EM structure of the Escherichia coli 70S ribosome in complex with antibiotic Avilamycin C, mRNA and P-site tRNA at 3.6A resolution Deposited 2016-06-06	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric	Chain 1Z 1–94（94 aa）	Not recorded	6UQ (2R,3S,4R,6S)-4-hydroxy-6-{[(2R,3aR,4R,4'R,5'S,6S,6'R,7aR)-4'-hydroxy-6-{[(2S,3R,4R,5S,6R)-3-hydroxy-2-{[(2R,3S,4S,5S,6S)-4-hydroxy-6-({(2R,3aS,3a'R,6S,6'R,7R,7'R,7aR,7a'R)-7'-hydroxy-7'-[(1S)-1-hydroxyethyl]-6'-methyl-7-[(2-methylpropanoyl)oxy]octahydro-4H-2,4'-spirobi[[1,3]dioxolo[4,5-c]pyran]-6-yl}oxy)-5-methoxy-2-(methoxymethyl)tetrahydro-2H-pyran-3-yl]oxy}-5-methoxy-6-methyltetrahydro-2H-pyran-4-yl]oxy}-4,6',7a-trimethyloctahydro-4H-spiro[1,3-dioxolo[4,5-c]pyran-2,2'-pyran]-5'-yl]oxy}-2-methyltetrahydro-2H-pyran-3-yl 3,5-dichloro-4-hydroxy-2-methoxy-6-methylbenzoate (non-preferred name) × 1 ZN ZINC ION × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE X-ray crystallization conditions pH 7.4	Resolution 3.60 Å
5KCS Cryo-EM structure of the Escherichia coli 70S ribosome in complex with antibiotic Evernimycin, mRNA, TetM and P-site tRNA at 3.9A resolution Deposited 2016-06-06	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 53 PDB declaration: 58-meric	Chain 1Z 1–94（94 aa）	Not recorded	EVN (2R,3R,4R,6S)-6-{[(2R,3aR,4R,4'R,5'S,6S,6'R,7S,7aR)-6-{[(2S,3R,4R,5S,6R)-2-{[(2R,3S,4S,5S,6S)-6-({(2R,3aS,3a'R,6S,7R,7' R,7aS,7a'S)-7'-[(2,4-dihydroxy-6-methylbenzoyl)oxy]-7-hydroxyoctahydro-4H-2,4'-spirobi[[1,3]dioxolo[4,5-c]pyran]-6-yl}ox y)-4-hydroxy-5-methoxy-2-(methoxymethyl)tetrahydro-2H-pyran-3-yl]oxy}-3-hydroxy-5-methoxy-6-methyltetrahydro-2H-pyran-4- yl]oxy}-4',7-dihydroxy-4,6',7a-trimethyloctahydro-4H-spiro[1,3-dioxolo[4,5-c]pyran-2,2'-pyran]-5'-yl]oxy}-4-{[(2R,4S,5R, 6S)-5-methoxy-4,6-dimethyl-4-nitrotetrahydro-2H-pyran-2-yl]oxy}-2-methyltetrahydro-2H-pyran-3-yl 3,5-dichloro-4-hydroxy-2-methoxy-6-methylbenzoate (non-preferred name) × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.90 Å
5KPS Structure of RelA bound to ribosome in absence of A/R tRNA (Structure I) Deposited 2016-07-05	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 58-meric	Chain V 1–94（94 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE；Apply 2 uL sample and blot 4 seconds before plunging into liquid ethane (GATAN CRYOPLUNGE 3).	Resolution 3.90 Å
5KPV Structure of RelA bound to ribosome in presence of A/R tRNA (Structure II) Deposited 2016-07-05	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 59-meric	Chain U 1–94（94 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE；Apply 2 uL sample and blot 4 seconds before plunging into liquid ethane (GATAN CRYOPLUNGE 3).	Resolution 4.10 Å
5KPW Structure of RelA bound to ribosome in presence of A/R tRNA (Structure III) Deposited 2016-07-05	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 59-meric	Chain U 1–94（94 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE；Apply 2 uL sample and blot 4 seconds before plunging into liquid ethane (GATAN CRYOPLUNGE 3).	Resolution 3.90 Å
5KPX Structure of RelA bound to ribosome in presence of A/R tRNA (Structure IV) Deposited 2016-07-05	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 59-meric	Chain U 1–94（94 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE；Apply 2 uL sample and blot 4 seconds before plunging into liquid ethane (GATAN CRYOPLUNGE 3).	Resolution 3.90 Å
5L3P Cryo-EM structure of stringent response factor RelA bound to ErmCL-stalled ribosome complex Deposited 2016-05-24	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 58-meric	Chain Z 1–94（94 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.70 Å
5LZA Structure of the 70S ribosome with SECIS-mRNA and P-site tRNA (Initial complex, IC) Deposited 2016-09-29	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric	Chain V 1–94（94 aa）	Not recorded	ZN ZINC ION × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5；50 mM Hepes-KOH, pH 7.5, 70 mM NH4Cl, 30 mM KCl, 7 mM MgCl2, 0.6mM spermine, 0.4mM spermidine, 2 mM DTT cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.60 Å
5LZB Structure of SelB-Sec-tRNASec bound to the 70S ribosome in the initial binding state (IB) Deposited 2016-09-29	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 58-meric	Chain V 1–94（94 aa）	Not recorded	FME N-FORMYLMETHIONINE × 1 SEC SELENOCYSTEINE × 1 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 ZN ZINC ION × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5；50 mM Hepes-KOH, pH 7.5, 70 mM NH4Cl, 30 mM KCl, 7 mM MgCl2, 0.6mM spermine, 0.4mM spermidine, 2 mM DTT cryo-EM vitrification conditions Cryogen ETHANE	Resolution 5.30 Å
5LZC Structure of SelB-Sec-tRNASec bound to the 70S ribosome in the codon reading state (CR) Deposited 2016-09-29	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 58-meric	Chain V 1–94（94 aa）	Not recorded	FME N-FORMYLMETHIONINE × 1 SEC SELENOCYSTEINE × 1 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 ZN ZINC ION × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5；50 mM Hepes-KOH, pH 7.5, 70 mM NH4Cl, 30 mM KCl, 7 mM MgCl2, 0.6mM spermine, 0.4mM spermidine, 2 mM DTT cryo-EM vitrification conditions Cryogen ETHANE	Resolution 4.80 Å
5LZD Structure of SelB-Sec-tRNASec bound to the 70S ribosome in the GTPase activated state (GA) Deposited 2016-09-29	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 58-meric	Chain V 1–94（94 aa）	Not recorded	CL CHLORIDE ION × 1 MG MAGNESIUM ION × 147 FME N-FORMYLMETHIONINE × 1 SEC SELENOCYSTEINE × 1 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 ZN ZINC ION × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5；50 mM Hepes-KOH, pH 7.5, 70 mM NH4Cl, 30 mM KCl, 7 mM MgCl2, 0.6mM spermine, 0.4mM spermidine, 2 mM DTT cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.40 Å
5LZE Structure of the 70S ribosome with Sec-tRNASec in the classical pre-translocation state (C) Deposited 2016-09-29	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 57-meric	Chain V 1–94（94 aa）	Not recorded	ZN ZINC ION × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5；50 mM Hepes-KOH, pH 7.5, 70 mM NH4Cl, 30 mM KCl, 7 mM MgCl2, 0.6 mM spermine, 0.4 mM spermidine, 2 mM DTT cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.50 Å
5LZF Structure of the 70S ribosome with fMetSec-tRNASec in the hybrid pre-translocation state (H) Deposited 2016-09-29	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric	Chain V 1–94（94 aa）	Not recorded	ZN ZINC ION × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5；50 mM Hepes-KOH, pH 7.5, 70 mM NH4Cl, 30 mM KCl, 7 mM MgCl2, 0.6mM spermine, 0.4mM spermidine, 2 mM DTT cryo-EM vitrification conditions Cryogen ETHANE	Resolution 4.60 Å
5MDV Structure of ArfA and RF2 bound to the 70S ribosome (accommodated state) Deposited 2016-11-13	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 53 PDB declaration: 58-meric	Chain V 1–94（94 aa）	Not recorded	MG MAGNESIUM ION × 440 FME N-FORMYLMETHIONINE × 1 ZN ZINC ION × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.97 Å
5MDW Structure of ArfA(A18T) and RF2 bound to the 70S ribosome (pre-accommodated state) Deposited 2016-11-13	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 53 PDB declaration: 58-meric	Chain V 1–94（94 aa）	Not recorded	MG MAGNESIUM ION × 438 FME N-FORMYLMETHIONINE × 1 ZN ZINC ION × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.06 Å
5MDY Structure of ArfA and TtRF2 bound to the 70S ribosome (pre-accommodated state) Deposited 2016-11-13	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 53 PDB declaration: 62-meric	Chain V 1–94（94 aa）	Not recorded	MG MAGNESIUM ION × 450 FME N-FORMYLMETHIONINE × 1 ZN ZINC ION × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.35 Å
5MDZ Structure of the 70S ribosome (empty A site) Deposited 2016-11-13	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 56-meric	Chain V 1–94（94 aa）	Not recorded	MG MAGNESIUM ION × 437 FME N-FORMYLMETHIONINE × 1 ZN ZINC ION × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.10 Å
5MGP Structural basis for ArfA-RF2 mediated translation termination on stop-codon lacking mRNAs Deposited 2016-11-21	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 56-meric	Chain V 1–94（94 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.2 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.10 Å
5NCO Quaternary complex between SRP, SR, and SecYEG bound to the translating ribosome Deposited 2017-03-06	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 36 PDB declaration: 40-meric	Chain W 1–94（94 aa）	Not recorded	ZN ZINC ION × 1 ALF TETRAFLUOROALUMINATE ION × 2 MG MAGNESIUM ION × 2 GDP GUANOSINE-5'-DIPHOSPHATE × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 4.80 Å
5NP6 70S structure prior to bypassing Deposited 2017-04-13	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 57-meric	Chain t 1–94（94 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.60 Å
5NWY 2.9 A cryo-EM structure of VemP-stalled ribosome-nascent chain complex Deposited 2017-05-08	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 56-meric	Chain i 1–94（94 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.2 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.90 Å
5O2R Cryo-EM structure of the proline-rich antimicrobial peptide Api137 bound to the terminating ribosome Deposited 2017-05-22	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 53 PDB declaration: 58-meric	Chain V 1–94（94 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.40 Å
5U4I Structural Basis of Co-translational Quality Control by ArfA and RF2 Bound to Ribosome Deposited 2016-12-04	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 57-meric	Chain W 1–94（94 aa）	Not recorded	MG MAGNESIUM ION × 124 ZN ZINC ION × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.50 Å
5UYK 70S ribosome bound with cognate ternary complex not base-paired to A site codon (Structure I) Deposited 2017-02-24	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 53 PDB declaration: 60-meric	Chain 24 1–94（94 aa）	Not recorded	GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE；2 uL of complex was applied to each grid. After a 10-second incubation, the grids were blotted for 2 to 4 seconds.	Resolution 3.90 Å
5UYL 70S ribosome bound with cognate ternary complex base-paired to A site codon (Structure II) Deposited 2017-02-24	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 53 PDB declaration: 60-meric	Chain 24 1–94（94 aa）	Not recorded	FME N-FORMYLMETHIONINE × 1 PHE PHENYLALANINE × 1 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE；2 uL of complex was applied to each grid. After a 10-second incubation, the grids were blotted for 2 to 4 seconds.	Resolution 3.60 Å
5UYM 70S ribosome bound with cognate ternary complex base-paired to A site codon, closed 30S (Structure III) Deposited 2017-02-24	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 53 PDB declaration: 60-meric	Chain 24 1–94（94 aa）	Not recorded	MG MAGNESIUM ION × 383 ZN ZINC ION × 2 FME N-FORMYLMETHIONINE × 1 PHE PHENYLALANINE × 1 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE；2 uL of complex was applied to each grid. After a 10-second incubation, the grids were blotted for 2 to 4 seconds.	Resolution 3.20 Å
5UYN 70S ribosome bound with near-cognate ternary complex not base-paired to A site codon (Structure I-nc) Deposited 2017-02-24	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 53 PDB declaration: 60-meric	Chain 24 1–94（94 aa）	Not recorded	GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE；2 uL of complex was applied to each grid. After a 10-second incubation, the grids were blotted for 2 to 4 seconds.	Resolution 4.00 Å
5UYP 70S ribosome bound with near-cognate ternary complex base-paired to A site codon, open 30S (Structure II-nc) Deposited 2017-02-24	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 53 PDB declaration: 60-meric	Chain 24 1–94（94 aa）	Not recorded	FME N-FORMYLMETHIONINE × 1 LYS LYSINE × 1 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE；2 uL of complex was applied to each grid. After a 10-second incubation, the grids were blotted for 2 to 4 seconds.	Resolution 3.90 Å
5UYQ 70S ribosome bound with near-cognate ternary complex base-paired to A site codon, closed 30S (Structure III-nc) Deposited 2017-02-24	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 53 PDB declaration: 60-meric	Chain 24 1–94（94 aa）	Not recorded	FME N-FORMYLMETHIONINE × 1 LYS LYSINE × 1 MG MAGNESIUM ION × 1 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE；2 uL of complex was applied to each grid. After a 10-second incubation, the grids were blotted for 2 to 4 seconds.	Resolution 3.80 Å
5WDT 70S ribosome-EF-Tu H84A complex with GppNHp Deposited 2017-07-06	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 59-meric	Chain V 2–93（92 aa）	Not recorded	FME N-FORMYLMETHIONINE × 1 MG MAGNESIUM ION × 1904 K POTASSIUM ION × 5 PHE PHENYLALANINE × 1 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE；5 second blot time, no wait time	Resolution 3.00 Å
5WE4 70S ribosome-EF-Tu wt complex with GppNHp Deposited 2017-07-07	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 59-meric	Chain V 2–93（92 aa）	Not recorded	MG MAGNESIUM ION × 1618 K POTASSIUM ION × 1 FME N-FORMYLMETHIONINE × 1 PHE PHENYLALANINE × 1 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE；5 second blot time, no wait time	Resolution 3.10 Å
5WE6 70S ribosome-EF-Tu H84A complex with GTP and cognate tRNA Deposited 2017-07-07	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 59-meric	Chain V 2–93（92 aa）	Not recorded	FME N-FORMYLMETHIONINE × 1 MG MAGNESIUM ION × 662 K POTASSIUM ION × 8 PHE PHENYLALANINE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE；5 second blot time, no wait time	Resolution 3.40 Å
5WFK 70S ribosome-EF-Tu H84A complex with GTP and near-cognate tRNA (Complex C3) Deposited 2017-07-12	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 59-meric	Chain V 2–93（92 aa）	Not recorded	FME N-FORMYLMETHIONINE × 1 MG MAGNESIUM ION × 1413 K POTASSIUM ION × 1 PHE PHENYLALANINE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE；5 second blot time, no wait time	Resolution 3.40 Å
6BU8 70S ribosome with S1 domains 1 and 2 (Class 1) Deposited 2017-12-08	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 53 PDB declaration: 60-meric	Chain 24 1–94（94 aa）	Not recorded	FME N-FORMYLMETHIONINE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE；2 uL of complex was applied to each grid. After a 10-second incubation, the grids were blotted for 2 to 4 seconds.	Resolution 3.50 Å
6BY1 E. coli pH03H9 complex Deposited 2017-12-19	Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 57-meric	Chain CV 1–94（94 aa）	Not recorded	MG MAGNESIUM ION × 225 ZN ZINC ION × 2	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；289 K；PEG 2000, PEP629, potassium thiocyanate, tris acetate	Resolution 3.94 Å R-free 0.269
6BY1 E. coli pH03H9 complex Deposited 2017-12-19	Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric	Chain DV 1–94（94 aa）	Not recorded	MG MAGNESIUM ION × 220 ZN ZINC ION × 2	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；289 K；PEG 2000, PEP629, potassium thiocyanate, tris acetate	Resolution 3.94 Å R-free 0.269
6C4I Conformation of methylated GGQ in the peptidyl transferase center during translation termination Deposited 2018-01-12	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 53 PDB declaration: 59-meric	Chain W 1–94（94 aa）	Not recorded	MG MAGNESIUM ION × 317 ZN ZINC ION × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE；Grids were blotted for 3.5 seconds.	Resolution 3.24 Å
6DNC E.coli RF1 bound to E.coli 70S ribosome in response to UAU sense A-site codon Deposited 2018-06-06	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 53 PDB declaration: 59-meric	Chain Z 1–94（94 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7；20 mM Tris-HCl, 100 mM NH4Cl, 20.5 mM MgCl2, 0.5 mM EDTA, 6 mM beta-mercaptoethanol cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.70 Å
6ENF Cryo-EM structure of a polyproline-stalled ribosome in the absence of EF-P Deposited 2017-10-04	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 53-meric	Chain V 1–94（94 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.20 Å
6ENJ Polyproline-stalled ribosome in the presence of A+P site tRNA and elongation-factor P (EF-P) Deposited 2017-10-05	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric	Chain V 1–94（94 aa）	Not recorded	PRO PROLINE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.70 Å
6ENU Polyproline-stalled ribosome in the presence of elongation-factor P (EF-P) Deposited 2017-10-06	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 56-meric	Chain V 1–94（94 aa）	Not recorded	PRO PROLINE × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.10 Å
6GBZ 50S ribosomal subunit assembly intermediate state 5 Deposited 2018-04-16	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 27 PDB declaration: 29-meric	Chain V 1–94（94 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.80 Å
6GC0 50S ribosomal subunit assembly intermediate state 4 Deposited 2018-04-16	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 26 PDB declaration: 28-meric	Chain V 1–94（94 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.80 Å
6GC4 50S ribosomal subunit assembly intermediate state 3 Deposited 2018-04-17	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 24 PDB declaration: 26-meric	Chain V 1–94（94 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 4.30 Å
6GC8 50S ribosomal subunit assembly intermediate - 50S rec* Deposited 2018-04-17	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 27 PDB declaration: 29-meric	Chain V 1–94（94 aa）	Not recorded	ZN ZINC ION × 1 MG MAGNESIUM ION × 5	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.80 Å
6GWT Cryo-EM structure of an E. coli 70S ribosome in complex with RF3-GDPCP, RF1(GAQ) and Pint-tRNA (State I) Deposited 2018-06-25	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 53 PDB declaration: 58-meric	Chain V 1–94（94 aa）	Not recorded	GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4；Solutions were preapred fresh and filtered previous to usage. cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.80 Å
6GXM Cryo-EM structure of an E. coli 70S ribosome in complex with RF3-GDPCP, RF1(GAQ) and Pint-tRNA (State II) Deposited 2018-06-27	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 53 PDB declaration: 58-meric	Chain V 1–94（94 aa）	Not recorded	GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4；Solutions were preapred fresh and filtered previous to usage. cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.80 Å
6GXN Cryo-EM structure of an E. coli 70S ribosome in complex with RF3-GDPCP, RF1(GAQ) and Pint-tRNA (State III) Deposited 2018-06-27	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 53 PDB declaration: 58-meric	Chain V 1–94（94 aa）	Not recorded	GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4；Solutions were preapred fresh and filtered previous to usage. cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.90 Å
6GXO Cryo-EM structure of a rotated E. coli 70S ribosome in complex with RF3-GDPCP, RF1(GAQ) and P/E-tRNA (State IV) Deposited 2018-06-27	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 53 PDB declaration: 58-meric	Chain V 1–94（94 aa）	Not recorded	GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4；Solutions were preapred fresh and filtered previous to usage. cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.90 Å
6GXP Cryo-EM structure of a rotated E. coli 70S ribosome in complex with RF3-GDPCP(RF3-only) Deposited 2018-06-27	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 55-meric	Chain V 1–94（94 aa）	Not recorded	GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4；Solutions were preapred fresh and filtered previous to usage. cryo-EM vitrification conditions Cryogen ETHANE	Resolution 4.40 Å
6H4N Structure of a hibernating 100S ribosome reveals an inactive conformation of the ribosomal protein S1 - 70S Hibernating E. coli Ribosome Deposited 2018-07-22	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 57-meric	Chain V 1–94（94 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5；B100S buffer (25 mM Hepes pH 7.5, 100 mM KOAc, 15 mM Mg(OAc)2, 1 mM dithiothreitol) cryo-EM vitrification conditions Cryogen ETHANE-PROPANE	Resolution 3.00 Å
6H58 Structure of a hibernating 100S ribosome reveals an inactive conformation of the ribosomal protein S1 - Full 100S Hibernating E. coli Ribosome Deposited 2018-07-24	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 104 PDB declaration: 112-meric	Chain V 1–94（94 aa） Chain VV 1–94（94 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE-PROPANE	Resolution 7.90 Å
6HRM E. coli 70S d2d8 stapled ribosome Deposited 2018-09-27	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 53-meric	Chain V 1–94（94 aa）	Not recorded	MG MAGNESIUM ION × 436 ZN ZINC ION × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.96 Å
6I0Y TnaC-stalled ribosome complex with the titin I27 domain folding close to the ribosomal exit tunnel Deposited 2018-10-26	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 33 PDB declaration: 36-meric	Chain 8 1–94（94 aa）	Not recorded	MG MAGNESIUM ION × 143 ZN ZINC ION × 1 TRP TRYPTOPHAN × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.20 Å
6I7V Ribosomal protein paralogs bL31 and bL36 Deposited 2018-11-19	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 99 PDB declaration: 105-meric	Chain CW 1–94（94 aa） Chain DW 1–94（94 aa）	Not recorded	MG MAGNESIUM ION × 453 PGE TRIETHYLENE GLYCOL × 8 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 13 PG4 TETRAETHYLENE GLYCOL × 5 SPD SPERMIDINE × 3 PUT 1,4-DIAMINOBUTANE × 7 1PE PENTAETHYLENE GLYCOL × 2 ACY ACETIC ACID × 3 PEG DI(HYDROXYETHYL)ETHER × 9 EDO 1,2-ETHANEDIOL × 12 GUN GUANINE × 1	X-RAY DIFFRACTION X-ray crystallization conditions MICROBATCH；pH 6.5；291 K；PEG8k, MPD, KSCN	Resolution 2.90 Å R-free 0.255
6OFX Non-rotated ribosome (Structure I) Deposited 2019-04-01	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 54-meric	Chain v 1–94（94 aa）	Not recorded	FME N-FORMYLMETHIONINE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.30 Å
6OG7 70S termination complex with RF2 bound to the UGA codon. Non-rotated ribosome with RF2 bound (Structure II) Deposited 2019-04-01	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric	Chain v 1–94（94 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.30 Å
6Q97 Structure of tmRNA SmpB bound in A site of E. coli 70S ribosome Deposited 2018-12-17	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 53 PDB declaration: 60-meric	Chain V 1–94（94 aa）	Not recorded	MG MAGNESIUM ION × 439 ZN ZINC ION × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.90 Å
6Q98 Structure of tmRNA SmpB bound in P site of E. coli 70S ribosome Deposited 2018-12-17	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 53 PDB declaration: 57-meric	Chain V 1–94（94 aa）	Not recorded	MG MAGNESIUM ION × 429 ZN ZINC ION × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 4.30 Å
6Q9A Structure of tmRNA SmpB bound past E site of E. coli 70S ribosome Deposited 2018-12-17	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 53 PDB declaration: 58-meric	Chain V 1–93（93 aa）	Not recorded	MG MAGNESIUM ION × 437 ZN ZINC ION × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.70 Å
6QUL Structure of a bacterial 50S ribosomal subunit in complex with the novel quinoxolidinone antibiotic cadazolid Deposited 2019-02-27	Different oligomeric state Different ligand/ion Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 28 PDB declaration: 31-meric	Chain W 1–94（94 aa）	Not recorded	FME N-FORMYLMETHIONINE × 1 MG MAGNESIUM ION × 382 AMP ADENOSINE MONOPHOSPHATE × 1 JJH cadazolid × 1 ZN ZINC ION × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE-PROPANE	Resolution 3.00 Å
6S0K Ribosome nascent chain in complex with SecA Deposited 2019-06-17	Different oligomeric state Different ligand/ion Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 32 PDB declaration: 35-meric	Chain W 1–94（94 aa）	Not recorded	MG MAGNESIUM ION × 68 ZN ZINC ION × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE-PROPANE	Resolution 3.10 Å
6SZS Release factor-dependent ribosome rescue by BrfA in the Gram-positive bacterium Bacillus subtilis Deposited 2019-10-02	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 56-meric	Chain V 1–94（94 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.2 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.06 Å
6TBV Cryo-EM structure of an Escherichia coli ribosome-SpeFL complex stalled in response to L-ornithine (Replicate 2) Deposited 2019-11-04	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric	Chain L251 1–94（94 aa）	Not recorded	MG MAGNESIUM ION × 339 K POTASSIUM ION × 147 UNX UNKNOWN LIGAND × 1201 ZN ZINC ION × 3 ORN L-ornithine × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.70 Å
6TC3 Cryo-EM structure of an Escherichia coli ribosome-SpeFL complex stalled in response to L-ornithine (Replicate 1) Deposited 2019-11-05	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric	Chain L251 1–94（94 aa）	Not recorded	MG MAGNESIUM ION × 339 K POTASSIUM ION × 147 UNX UNKNOWN LIGAND × 1201 ZN ZINC ION × 3 ORN L-ornithine × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.70 Å
6VWL 70S ribosome bound to HIV frameshifting stem-loop (FSS) and P/E tRNA (rotated conformation) Deposited 2020-02-20	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 53-meric	Chain T 1–94（94 aa）	Not recorded	MG MAGNESIUM ION × 34	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.10 Å
6VWM 70S ribosome bound to HIV frameshifting stem-loop (FSS) and P-site tRNA (non-rotated conformation, Structure I) Deposited 2020-02-20	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 53-meric	Chain T 1–94（94 aa）	Not recorded	MG MAGNESIUM ION × 53	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.40 Å
6VWN 70S ribosome bound to HIV frameshifting stem-loop (FSS) and P-site tRNA (non-rotated conformation, Structure II) Deposited 2020-02-20	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 53-meric	Chain T 1–94（94 aa）	Not recorded	MG MAGNESIUM ION × 55	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.40 Å
6WD6 Cryo-EM of elongating ribosome with EF-Tu*GTP elucidates tRNA proofreading (Cognate Structure II-C2) Deposited 2020-03-31	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 59-meric	Chain v 1–94（94 aa）	Not recorded	FME N-FORMYLMETHIONINE × 1 PHE PHENYLALANINE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.70 Å
6WDB Cryo-EM of elongating ribosome with EF-Tu*GTP elucidates tRNA proofreading (Cognate Structure IV-A) Deposited 2020-03-31	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 58-meric	Chain v 1–94（94 aa）	Not recorded	FME N-FORMYLMETHIONINE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 4.00 Å
6WDC Cryo-EM of elongating ribosome with EF-Tu*GTP elucidates tRNA proofreading (Cognate Structure IV-B) Deposited 2020-03-31	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 58-meric	Chain v 1–94（94 aa）	Not recorded	FME N-FORMYLMETHIONINE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 4.20 Å
6WDD Cryo-EM of elongating ribosome with EF-Tu*GTP elucidates tRNA proofreading (Cognate Structure V-A) Deposited 2020-03-31	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 58-meric	Chain v 1–94（94 aa）	Not recorded	FME N-FORMYLMETHIONINE × 1 PHE PHENYLALANINE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.20 Å
6WDE Cryo-EM of elongating ribosome with EF-Tu*GTP elucidates tRNA proofreading (Cognate Structure V-B) Deposited 2020-03-31	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 58-meric	Chain v 1–94（94 aa）	Not recorded	PHE PHENYLALANINE × 1 MET METHIONINE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.00 Å
6WDF Cryo-EM of elongating ribosome with EF-Tu*GTP elucidates tRNA proofreading (Cognate Structure VI-A) Deposited 2020-03-31	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 57-meric	Chain v 1–94（94 aa）	Not recorded	PHE PHENYLALANINE × 1 FME N-FORMYLMETHIONINE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.30 Å
6WDG Cryo-EM of elongating ribosome with EF-Tu*GTP elucidates tRNA proofreading (Cognate Structure VI-B) Deposited 2020-03-31	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 57-meric	Chain v 1–94（94 aa）	Not recorded	PHE PHENYLALANINE × 1 FME N-FORMYLMETHIONINE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.30 Å
6WDH Cryo-EM of elongating ribosome with EF-Tu*GTP elucidates tRNA proofreading (Non-cognate Structure IV-B1) Deposited 2020-03-31	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 58-meric	Chain v 1–94（94 aa）	Not recorded	FME N-FORMYLMETHIONINE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 4.30 Å
6WDI Cryo-EM of elongating ribosome with EF-Tu*GTP elucidates tRNA proofreading (Non-cognate Structure IV-B2) Deposited 2020-03-31	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 58-meric	Chain v 1–94（94 aa）	Not recorded	FME N-FORMYLMETHIONINE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 4.00 Å
6WDJ Cryo-EM of elongating ribosome with EF-Tu*GTP elucidates tRNA proofreading (Non-cognate Structure V-A1) Deposited 2020-03-31	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 58-meric	Chain v 1–94（94 aa）	Not recorded	FME N-FORMYLMETHIONINE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.70 Å
6WDK Cryo-EM of elongating ribosome with EF-Tu*GTP elucidates tRNA proofreading (Non-cognate Structure V-A2) Deposited 2020-03-31	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 58-meric	Chain v 1–94（94 aa）	Not recorded	FME N-FORMYLMETHIONINE × 1 PHE PHENYLALANINE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.60 Å
6WDL Cryo-EM of elongating ribosome with EF-Tu*GTP elucidates tRNA proofreading (Non-cognate Structure V-B1) Deposited 2020-03-31	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 58-meric	Chain v 1–94（94 aa）	Not recorded	FME N-FORMYLMETHIONINE × 1 PHE PHENYLALANINE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.70 Å
6WDM Cryo-EM of elongating ribosome with EF-Tu*GTP elucidates tRNA proofreading (Non-cognate Structure V-B2) Deposited 2020-03-31	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 58-meric	Chain v 1–94（94 aa）	Not recorded	PHE PHENYLALANINE × 1 FME N-FORMYLMETHIONINE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.60 Å
6WNT 50S ribosomal subunit without free 5S rRNA and perturbed PTC Deposited 2020-04-23	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 30 PDB declaration: 31-meric	Chain v 1–94（94 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.10 Å
6WNV 70S ribosome without free 5S rRNA and with a perturbed PTC Deposited 2020-04-23	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 52-meric	Chain v 1–94（94 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.50 Å
6WNW Active 70S ribosome without free 5S rRNA and bound with A- and P- tRNA Deposited 2020-04-23	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 57-meric	Chain v 1–94（94 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.20 Å
6XZ7 E. coli 50S ribosomal subunit in complex with dirithromycin, fMet-Phe-tRNA(Phe) and deacylated tRNA(iMet). Deposited 2020-02-03	Different oligomeric state Different ligand/ion Different experimental conditions	Assembly 1 Protein–RNA Heteromer；Protein × 29 PDB declaration: 33-meric	Chain V 1–94（94 aa）	Not recorded	MG MAGNESIUM ION × 178 DI0 Dirithromycin × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.10 Å
6XZA E. coli 70S ribosome in complex with dirithromycin, and deacylated tRNA(iMet) (focused classification). Deposited 2020-02-03	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric	Chain V2 1–94（94 aa）	Not recorded	DI0 Dirithromycin × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.66 Å
6XZB E. coli 70S ribosome in complex with dirithromycin, fMet-Phe-tRNA(Phe) and deacylated tRNA(iMet) (focused classification). Deposited 2020-02-03	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 54-meric	Chain V2 1–94（94 aa）	Not recorded	DI0 Dirithromycin × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.54 Å
6Y69 Cryo-EM structure of an Escherichia coli 70S ribosome in complex with antibiotic TetracenomycinX Deposited 2020-02-26	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 54-meric	Chain V 1–94（94 aa）	Not recorded	OCW Tetracenomycin X × 1 MG MAGNESIUM ION × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.86 Å
6YSR Structure of the P+9 stalled ribosome complex Deposited 2020-04-23	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 57-meric	Chain V 1–94（94 aa）	Not recorded	MG MAGNESIUM ION × 332 ZN ZINC ION × 2 CL CHLORIDE ION × 1 NA SODIUM ION × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4；50 mM HEPES, 30 mM KCl, 7 mM MgCl2 cryo-EM vitrification conditions Cryogen ETHANE；Manual blotting & plunge-freezing	Resolution 3.10 Å
6YSS Structure of the P+9 ArfB-ribosome complex in the post-hydrolysis state Deposited 2020-04-23	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 53 PDB declaration: 58-meric	Chain V 1–94（94 aa）	Not recorded	MG MAGNESIUM ION × 387 ZN ZINC ION × 2 NA SODIUM ION × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4；50 mM HEPES, 30 mM KCl, 7 mM MgCl2 cryo-EM vitrification conditions Cryogen ETHANE；Manual blotting & plunge-freezing	Resolution 2.60 Å
6YST Structure of the P+9 ArfB-ribosome complex with P/E hybrid tRNA in the post-hydrolysis state Deposited 2020-04-23	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 53 PDB declaration: 58-meric	Chain V 1–94（94 aa）	Not recorded	MG MAGNESIUM ION × 253 ZN ZINC ION × 2 NA SODIUM ION × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4；50 mM HEPES, 30 mM KCl, 7 mM MgCl2 cryo-EM vitrification conditions Cryogen ETHANE；Manual blotting & plunge-freezing	Resolution 3.20 Å
6YSU Structure of the P+0 ArfB-ribosome complex in the post-hydrolysis state Deposited 2020-04-23	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 57-meric	Chain V 1–94（94 aa）	Not recorded	MG MAGNESIUM ION × 356 ZN ZINC ION × 1 NA SODIUM ION × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4；50 mM HEPES, 30 mM KCl, 7 mM MgCl2, 0.1 % w/v DDM cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.70 Å
6ZTJ E. coli 70S-RNAP expressome complex in NusG-coupled state (38 nt intervening mRNA) Deposited 2020-07-20	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Other combination Heteromer；Protein × 58 PDB declaration: 66-meric	Chain BW 1–94（94 aa）	Not recorded	MG MAGNESIUM ION × 479 PHE PHENYLALANINE × 1 ZN ZINC ION × 4	ELECTRON MICROSCOPY cryo-EM buffer pH 8 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.40 Å
6ZTL E. coli 70S-RNAP expressome complex in collided state bound to NusG Deposited 2020-07-20	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Other combination Heteromer；Protein × 54 PDB declaration: 62-meric	Chain BW 1–94（94 aa）	Not recorded	MG MAGNESIUM ION × 441 PHE PHENYLALANINE × 1 ZN ZINC ION × 3	ELECTRON MICROSCOPY cryo-EM buffer pH 8 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.50 Å
6ZTM E. coli 70S-RNAP expressome complex in collided state without NusG Deposited 2020-07-20	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Other combination Heteromer；Protein × 52 PDB declaration: 60-meric	Chain BW 1–94（94 aa）	Not recorded	MG MAGNESIUM ION × 421 PHE PHENYLALANINE × 1 ZN ZINC ION × 3	ELECTRON MICROSCOPY cryo-EM buffer pH 8 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.30 Å
6ZTN E. coli 70S-RNAP expressome complex in NusG-coupled state (42 nt intervening mRNA) Deposited 2020-07-20	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Other combination Heteromer；Protein × 54 PDB declaration: 63-meric	Chain BW 1–94（94 aa）	Not recorded	MG MAGNESIUM ION × 487 PHE PHENYLALANINE × 1 ZN ZINC ION × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 8 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.90 Å
6ZTO E. coli 70S-RNAP expressome complex in uncoupled state 1 Deposited 2020-07-20	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Other combination Heteromer；Protein × 54 PDB declaration: 62-meric	Chain BW 1–94（94 aa）	Not recorded	MG MAGNESIUM ION × 487 PHE PHENYLALANINE × 1 ZN ZINC ION × 4	ELECTRON MICROSCOPY cryo-EM buffer pH 8 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.00 Å
6ZTP E. coli 70S-RNAP expressome complex in uncoupled state 6 Deposited 2020-07-20	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Other combination Heteromer；Protein × 54 PDB declaration: 62-meric	Chain BW 1–94（94 aa）	Not recorded	MG MAGNESIUM ION × 487 PHE PHENYLALANINE × 1 ZN ZINC ION × 4	ELECTRON MICROSCOPY cryo-EM buffer pH 8 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.00 Å
6ZU1 E. coli 70S-RNAP expressome complex in uncoupled state 2 Deposited 2020-07-21	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Other combination Heteromer；Protein × 54 PDB declaration: 62-meric	Chain BW 1–94（94 aa）	Not recorded	MG MAGNESIUM ION × 487 PHE PHENYLALANINE × 1 ZN ZINC ION × 4	ELECTRON MICROSCOPY cryo-EM buffer pH 8 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.00 Å
7ABZ Structure of pre-accomodated trans-translation complex on E. coli stalled ribosome. Deposited 2020-09-09	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 58-meric	Chain V 1–94（94 aa）	Not recorded	MG MAGNESIUM ION × 192 KIR KIRROMYCIN × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ZN ZINC ION × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE；blot for 3.5 seconds before plunging	Resolution 3.21 Å
7AC7 Structure of accomodated trans-translation complex on E. Coli stalled ribosome. Deposited 2020-09-10	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 58-meric	Chain V 1–94（94 aa）	Not recorded	MG MAGNESIUM ION × 441 ZN ZINC ION × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE；blot for 3.5 seconds before plunging	Resolution 3.08 Å
7ACJ Structure of translocated trans-translation complex on E. coli stalled ribosome. Deposited 2020-09-11	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 55-meric	Chain V 1–94（94 aa）	Not recorded	MG MAGNESIUM ION × 308 ZN ZINC ION × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE；blot for 3.5 seconds before plunging	Resolution 3.20 Å
7ACR Structure of post-translocated trans-translation complex on E. coli stalled ribosome. Deposited 2020-09-11	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 55-meric	Chain V 1–94（94 aa）	Not recorded	MG MAGNESIUM ION × 212 ZN ZINC ION × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE；blot for 3.5 seconds before plunging	Resolution 3.44 Å
7B5K E. coli 70S containing suppressor tRNA in the A-site stabilized by a Negamycin analogue and P-site tRNA-nascent chain. Deposited 2020-12-03	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 57-meric	Chain V 1–94（94 aa）	Not recorded	MG MAGNESIUM ION × 359 ERY ERYTHROMYCIN A × 1 SY5 2-[[[(3~{R},5~{R})-3-azanyl-6-(3-azanylpropylamino)-5-oxidanyl-hexanoyl]amino]-methyl-amino]ethanoic acid × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE-PROPANE	Resolution 2.90 Å
7BL2 pre-50S-ObgE particle state 1 Deposited 2021-01-18	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 29 PDB declaration: 31-meric	Chain V 1–94（94 aa）	Not recorded	MG MAGNESIUM ION × 1 ZN ZINC ION × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.70 Å
7BL3 pre-50S-ObgE particle state 2 Deposited 2021-01-18	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 29 PDB declaration: 31-meric	Chain V 1–94（94 aa）	Not recorded	ZN ZINC ION × 1 MG MAGNESIUM ION × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.50 Å
7BL4 in vitro reconstituted 50S-ObgE-GMPPNP-RsfS particle Deposited 2021-01-18	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 31 PDB declaration: 33-meric	Chain V 1–94（94 aa）	Not recorded	ZN ZINC ION × 2 MG MAGNESIUM ION × 6 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.40 Å
7BL5 pre-50S-ObgE particle Deposited 2021-01-18	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 33 PDB declaration: 35-meric	Chain V 1–94（94 aa）	Not recorded	GDP GUANOSINE-5'-DIPHOSPHATE × 1 MG MAGNESIUM ION × 2 ZN ZINC ION × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.30 Å
7BL6 50S-ObgE-GMPPNP particle Deposited 2021-01-18	Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 30 PDB declaration: 32-meric	Chain V 1–94（94 aa）	Not recorded	ZN ZINC ION × 2 MG MAGNESIUM ION × 2 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 4.00 Å
7BV8 Mature 50S ribosomal subunit from RrmJ knock out E.coli strain Deposited 2020-04-09	Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 30 PDB declaration: 32-meric	Chain W 1–94（94 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5；Solutions were made fresh form concentrated to avoid microbial contamination. cryo-EM vitrification conditions Cryogen ETHANE；blot for 2 seconds before plunging	Resolution 3.14 Å
7D6Z Molecular model of the cryo-EM structure of 70S ribosome in complex with peptide deformylase and trigger factor Deposited 2020-10-02	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 58-meric	Chain V 1–94（94 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.40 Å
7D80 Molecular model of the cryo-EM structure of 70S ribosome in complex with peptide deformylase, trigger factor, and methionine aminopeptidase Deposited 2020-10-06	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 57-meric	Chain t 1–94（94 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 4.10 Å
7JSS ArfB Rescue of a 70S Ribosome stalled on truncated mRNA with a partial A-site codon (+2-II) Deposited 2020-08-15	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 54-meric	Chain v 1–94（94 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.70 Å
7JSW ArfB Rescue of a 70S Ribosome stalled on truncated mRNA with a partial A-site codon (+2-III) Deposited 2020-08-16	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 54-meric	Chain v 1–94（94 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.80 Å
7JSZ ArfB Rescue of a 70S Ribosome stalled on truncated mRNA with a partial A-site codon (+2-IV) Deposited 2020-08-16	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 57-meric	Chain v 1–94（94 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.70 Å
7JT1 70S ribosome stalled on long mRNA with ArfB-1 and ArfB-2 bound (+9-III) Deposited 2020-08-16	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric	Chain v 1–94（94 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.30 Å
7JT2 70S ribosome stalled on long mRNA with ArfB bound in the A site Deposited 2020-08-16	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric	Chain v 1–94（94 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.50 Å
7JT3 Rotated 70S ribosome stalled on long mRNA with ArfB-1 and ArfB-2 bound in the A site (+9-IV) Deposited 2020-08-17	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 53 PDB declaration: 58-meric	Chain v 1–94（94 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.70 Å
7K50 Pre-translocation non-frameshifting(CCA-A) complex (Structure I) Deposited 2020-09-16	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 59-meric	Chain v 1–94（94 aa）	Not recorded	FME N-FORMYLMETHIONINE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE；Blotting force 9, blotted for 4 seconds	Resolution 3.40 Å
7K51 Mid-translocated non-frameshifting(CCA-A) complex with EF-G and GDPCP (Structure II) Deposited 2020-09-16	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 53 PDB declaration: 59-meric	Chain v 1–94（94 aa）	Not recorded	FME N-FORMYLMETHIONINE × 1 PRO PROLINE × 1 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE；Blotting force 9, blotted for 4 seconds	Resolution 3.50 Å
7K52 Near post-translocated non-frameshifting(CCA-A) complex with EF-G and GDPCP (Structure III) Deposited 2020-09-16	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 53 PDB declaration: 59-meric	Chain v 1–94（94 aa）	Not recorded	FME N-FORMYLMETHIONINE × 1 PRO PROLINE × 1 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE；blotting force 9, blotted for 4 seconds	Resolution 3.40 Å
7K53 Pre-translocation +1-frameshifting(CCC-A) complex (Structure I-FS) Deposited 2020-09-16	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 59-meric	Chain v 1–94（94 aa）	Not recorded	FME N-FORMYLMETHIONINE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE；Blotting force 9, blotted for 4 seconds	Resolution 3.20 Å
7K54 Mid-translocated +1-frameshifting(CCC-A) complex with EF-G and GDPCP (Structure II-FS) Deposited 2020-09-16	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 53 PDB declaration: 59-meric	Chain v 1–94（94 aa）	Not recorded	FME N-FORMYLMETHIONINE × 1 PRO PROLINE × 1 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE；Blotting force 9, blotted for 4 seconds	Resolution 3.20 Å
7K55 Near post-translocated +1-frameshifting(CCC-A) complex with EF-G and GDPCP (Structure III-FS) Deposited 2020-09-16	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 53 PDB declaration: 59-meric	Chain v 1–94（94 aa）	Not recorded	FME N-FORMYLMETHIONINE × 1 PRO PROLINE × 1 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE；blotting force 9, blotted for 4 seconds	Resolution 3.30 Å
7LV0 Pre-translocation rotated ribosome +1-frameshifting(CCC-A) complex (Structure Irot-FS) Deposited 2021-02-23	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 58-meric	Chain v 1–94（94 aa）	Not recorded	FME N-FORMYLMETHIONINE × 1 PRO PROLINE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE；Blotting force 9, blotted for 4 seconds	Resolution 3.20 Å
7N1P Elongating 70S ribosome complex in a classical pre-translocation (PRE-C) conformation Deposited 2021-05-28	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 58-meric	Chain LY 1–94（94 aa）	Not recorded	PUT 1,4-DIAMINOBUTANE × 18 MG MAGNESIUM ION × 405 ZN ZINC ION × 3 ATP ADENOSINE-5'-TRIPHOSPHATE × 3 SPD SPERMIDINE × 5	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.33 Å
7N2C Elongating 70S ribosome complex in a fusidic acid-stalled intermediate state of translocation bound to EF-G(GDP) (INT2) Deposited 2021-05-28	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 55 PDB declaration: 61-meric	Chain LY 1–94（94 aa）	Not recorded	PUT 1,4-DIAMINOBUTANE × 21 MG MAGNESIUM ION × 197 ZN ZINC ION × 3 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 SPD SPERMIDINE × 3 FUA FUSIDIC ACID × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.72 Å
7N2U Elongating 70S ribosome complex in a hybrid-H1 pre-translocation (PRE-H1) conformation Deposited 2021-05-29	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric	Chain LY 1–94（94 aa）	Not recorded	PUT 1,4-DIAMINOBUTANE × 16 MG MAGNESIUM ION × 368 ZN ZINC ION × 3 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 SPD SPERMIDINE × 5	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.53 Å
7N2V Elongating 70S ribosome complex in a spectinomycin-stalled intermediate state of translocation bound to EF-G in an active, GTP conformation (INT1) Deposited 2021-05-29	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 53 PDB declaration: 59-meric	Chain LY 1–94（94 aa）	Not recorded	SCM SPECTINOMYCIN × 3 PUT 1,4-DIAMINOBUTANE × 17 MG MAGNESIUM ION × 344 ZN ZINC ION × 3 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 SPD SPERMIDINE × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.54 Å
7N30 Elongating 70S ribosome complex in a hybrid-H2* pre-translocation (PRE-H2*) conformation Deposited 2021-05-30	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric	Chain LY 1–94（94 aa）	Not recorded	PUT 1,4-DIAMINOBUTANE × 11 MG MAGNESIUM ION × 296 ZN ZINC ION × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 SPD SPERMIDINE × 4	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.66 Å
7N31 Elongating 70S ribosome complex in a post-translocation (POST) conformation Deposited 2021-05-31	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric	Chain LY 1–94（94 aa）	Not recorded	MG MAGNESIUM ION × 209 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 PUT 1,4-DIAMINOBUTANE × 9 SPD SPERMIDINE × 4 ZN ZINC ION × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.69 Å
7NBU Structure of the HigB1 toxin mutant K95A from Mycobacterium tuberculosis (Rv1955) and its target, the cspA mRNA, on the E. coli Ribosome. Deposited 2021-01-27	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Insufficient information Heteromer；Protein × 50 PDB declaration: 56-meric	Chain u 1–94（94 aa）	Not recorded	MG MAGNESIUM ION × 313 FME N-FORMYLMETHIONINE × 1 ZN ZINC ION × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE；blot for 2s before plunging	Resolution 3.11 Å
7NSO Structure of ErmDL-Erythromycin-stalled 70S E. coli ribosomal complex with P-tRNA Deposited 2021-03-08	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric	Chain V 1–94（94 aa）	Not recorded	ERY ERYTHROMYCIN A × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE-PROPANE	Resolution 2.90 Å
7NSP Structure of ErmDL-Erythromycin-stalled 70S E. coli ribosomal complex with A and P-tRNA Deposited 2021-03-08	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric	Chain V 1–94（94 aa）	Not recorded	ERY ERYTHROMYCIN A × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE-PROPANE	Resolution 3.50 Å
7NSQ Structure of ErmDL-Telithromycin-stalled 70S E. coli ribosomal complex with A and P-tRNA Deposited 2021-03-08	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric	Chain V 1–94（94 aa）	Not recorded	TEL TELITHROMYCIN × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE-PROPANE	Resolution 3.10 Å
7NWT Initiated 70S ribosome in complex with 2A protein from encephalomyocarditis virus (EMCV) Deposited 2021-03-17	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 54 PDB declaration: 59-meric	Chain V 1–94（94 aa）	Not recorded	MG MAGNESIUM ION × 437 FME N-FORMYLMETHIONINE × 1 ZN ZINC ION × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5；Initiated 70S ribosomes in 50 mM Tris-HCl pH 7.5, 70 mM NH4Cl, 30 mM KCl, 7 mM MgCl2 were diluted tenfold into 20 mM HEPES pH 7.5, 100 mM potassium acetate, 1.5 mM MgCl2, 2.0 mM DTT. 2A protein was dialysed (3K MWCO, 277K, 16 h) into the same buffer. Crosslinking reactions of 50 microliters comprising 75 nM ribosomes, 3.0 micromolar 2A and 2.0 mM bis(sulfosuccinimidyl)suberate (BS3) were performed on ice (30 min) immediately prior to grid preparation. cryo-EM vitrification conditions Cryogen ETHANE；Quantifoil R 2/2 400-mesh copper supports were coated with an additional ~ 60 angstrom layer of amorphous, evaporated carbon by flotation and thoroughly dried before use. Grids were made hydrophilic by glow-discharge in air for 30 s. Three microliters of crosslinking reaction was applied to grids which were then blotted for 4.5 s and vitrified by plunging into liquid ethane using a Vitrobot MK IV (FEI) at 277K, 100% relative humidity.	Resolution 2.66 Å
7NWW CspA-27 cotranslational folding intermediate 1 Deposited 2021-03-17	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric	Chain U 1–94（94 aa）	Not recorded	MG MAGNESIUM ION × 413 ZN ZINC ION × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5；50 mM Tris-HCl, 70 mM NH4Cl, 30 mM KCl, 3.5 mM MgCl2, 8 mM putrescine, 0.5 mM spermidine, 1 mM DTT, 1 mM GTP, pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.05 Å
7O19 Cryo-EM structure of an Escherichia coli TnaC-ribosome complex stalled in response to L-tryptophan Deposited 2021-03-29	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric	Chain BV 1–94（94 aa）	Not recorded	MG MAGNESIUM ION × 170 ZN ZINC ION × 3 TRP TRYPTOPHAN × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5；All buffers used during the preparation contained 2 mM L-tryptophan. cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.90 Å
7O1A Cryo-EM structure of an Escherichia coli TnaC(R23F)-ribosome complex stalled in response to L-tryptophan Deposited 2021-03-29	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric	Chain BV 1–94（94 aa）	Not recorded	MG MAGNESIUM ION × 326 K POTASSIUM ION × 147 ZN ZINC ION × 3 TRP TRYPTOPHAN × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5；All buffers used during the preparation contained 2 mM L-tryptophan. cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.40 Å
7O1C Cryo-EM structure of an Escherichia coli TnaC(R23F)-ribosome-RF2 complex stalled in response to L-tryptophan Deposited 2021-03-29	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 56-meric	Chain BV 1–94（94 aa）	Not recorded	MG MAGNESIUM ION × 339 K POTASSIUM ION × 147 ZN ZINC ION × 3 TRP TRYPTOPHAN × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5；All buffers used during the preparation contained 2 mM L-tryptophan. cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.60 Å
7OIF CspA-27 cotranslational folding intermediate 2 Deposited 2021-05-11	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric	Chain U 1–94（94 aa）	Not recorded	MG MAGNESIUM ION × 416 ZN ZINC ION × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5；50 mM Tris-HCl, 70 mM NH4Cl, 30 mM KCl, 3.5 mM MgCl2, 8 mM putrescine, 0.5 mM spermidine, 1 mM DTT, 1 mM GTP, pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.00 Å
7OIG CspA-27 cotranslational folding intermediate 3 Deposited 2021-05-11	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric	Chain U 1–94（94 aa）	Not recorded	MG MAGNESIUM ION × 400 ZN ZINC ION × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5；50 mM Tris-HCl, 70 mM NH4Cl, 30 mM KCl, 3.5 mM MgCl2, 8 mM putrescine, 0.5 mM spermidine, 1 mM DTT, 1 mM GTP, pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.20 Å
7OII CspA-70 cotranslational folding intermediate 2 Deposited 2021-05-11	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric	Chain U 1–94（94 aa）	Not recorded	MG MAGNESIUM ION × 422 ZN ZINC ION × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5；50 mM Tris-HCl, 70 mM NH4Cl, 30 mM KCl, 3.5 mM MgCl2, 8 mM putrescine, 0.5 mM spermidine, 1 mM DTT, 1 mM GTP, pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.00 Å
7OIZ Cryo-EM structure of 70S ribosome stalled with TnaC peptide Deposited 2021-05-13	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric	Chain u 1–94（94 aa）	Not recorded	PAR PAROMOMYCIN × 1 MG MAGNESIUM ION × 308 TRP TRYPTOPHAN × 1 ZN ZINC ION × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.90 Å
7OJ0 Cryo-EM structure of 70S ribosome stalled with TnaC peptide and RF2 Deposited 2021-05-13	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 54 PDB declaration: 59-meric	Chain u 1–94（94 aa）	Not recorded	MG MAGNESIUM ION × 308 TRP TRYPTOPHAN × 1 ZN ZINC ION × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.50 Å
7OT5 CspA-70 cotranslational folding intermediate 1 Deposited 2021-06-09	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric	Chain U 1–94（94 aa）	Not recorded	MG MAGNESIUM ION × 394 ZN ZINC ION × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5；50 mM Tris-HCl, 70 mM NH4Cl, 30 mM KCl, 3.5 mM MgCl2, 8 mM putrescine, 0.5 mM spermidine, 1 mM DTT, 1 mM GTP, pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.90 Å
7P3K Cryo-EM structure of 70S ribosome stalled with TnaC peptide (control) Deposited 2021-07-08	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 54-meric	Chain u 1–94（94 aa）	Not recorded	MG MAGNESIUM ION × 308 CLM CHLORAMPHENICOL × 1 ZN ZINC ION × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.90 Å
7PJS Structure of the 70S ribosome with tRNAs in the classical pre-translocation state and apramycin (C) Deposited 2021-08-24	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 58-meric	Chain V 1–94（94 aa）	Not recorded	MG MAGNESIUM ION × 363 ZN ZINC ION × 2 NA SODIUM ION × 2 AM2 APRAMYCIN × 4	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5；50 mM HEPES, 70 mM NH4Cl, 30 mM KCl, 3.5 mM MgCl2, 0.6 mM spermine, 0.4 mM spermidine cryo-EM vitrification conditions Cryogen ETHANE；Manual blotting & plunge-freezing	Resolution 2.35 Å
7PJT Structure of the 70S ribosome with tRNAs in hybrid state 1 (H1) Deposited 2021-08-24	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 58-meric	Chain V 1–94（94 aa）	Not recorded	ZN ZINC ION × 2 AM2 APRAMYCIN × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE；Manual blotting & plunge-freezing	Resolution 6.00 Å
7PJV Structure of the 70S-EF-G-GDP-Pi ribosome complex with tRNAs in hybrid state 1 (H1-EF-G-GDP-Pi) Deposited 2021-08-24	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 53 PDB declaration: 59-meric	Chain V 1–94（94 aa）	Not recorded	MG MAGNESIUM ION × 369 ZN ZINC ION × 2 NA SODIUM ION × 2 AM2 APRAMYCIN × 3 GDP GUANOSINE-5'-DIPHOSPHATE × 1 PO4 PHOSPHATE ION × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5；50 mM HEPES, 70 mM NH4Cl, 30 mM KCl, 3.5 mM MgCl2, 0.6 mM spermine, 0.4 mM spermidine cryo-EM vitrification conditions Cryogen ETHANE；Manual blotting & plunge-freezing	Resolution 3.10 Å
7PJW Structure of the 70S-EF-G-GDP-Pi ribosome complex with tRNAs in hybrid state 2 (H2-EF-G-GDP-Pi) Deposited 2021-08-24	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 53 PDB declaration: 59-meric	Chain V 1–94（94 aa）	Not recorded	AM2 APRAMYCIN × 1 PO4 PHOSPHATE ION × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5；50 mM HEPES, 70 mM NH4Cl, 30 mM KCl, 3.5 mM MgCl2, 0.6 mM spermine, 0.4 mM spermidine cryo-EM vitrification conditions Cryogen ETHANE；Manual blotting & plunge-freezing	Resolution 4.00 Å
7PJX Structure of the 70S-EF-G-GDP ribosome complex with tRNAs in hybrid state 1 (H1-EF-G-GDP) Deposited 2021-08-24	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 53 PDB declaration: 59-meric	Chain V 1–94（94 aa）	Not recorded	AM2 APRAMYCIN × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5；50 mM HEPES, 70 mM NH4Cl, 30 mM KCl, 3.5 mM MgCl2, 0.6 mM spermine, 0.4 mM spermidine cryo-EM vitrification conditions Cryogen ETHANE；Manual blotting & plunge-freezing	Resolution 6.50 Å
7PJY Structure of the 70S-EF-G-GDP ribosome complex with tRNAs in chimeric state 1 (CHI1-EF-G-GDP) Deposited 2021-08-24	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 53 PDB declaration: 59-meric	Chain V 1–94（94 aa）	Not recorded	MG MAGNESIUM ION × 318 ZN ZINC ION × 2 NA SODIUM ION × 1 AM2 APRAMYCIN × 4 GDP GUANOSINE-5'-DIPHOSPHATE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5；50 mM HEPES, 70 mM NH4Cl, 30 mM KCl, 3.5 mM MgCl2, 0.6 mM spermine, 0.4 mM spermidine cryo-EM vitrification conditions Cryogen ETHANE；Manual blotting & plunge-freezing	Resolution 3.10 Å
7PJZ Structure of the 70S-EF-G-GDP ribosome complex with tRNAs in chimeric state 2 (CHI2-EF-G-GDP) Deposited 2021-08-24	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 53 PDB declaration: 59-meric	Chain V 1–94（94 aa）	Not recorded	ZN ZINC ION × 1 AM2 APRAMYCIN × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5；50 mM HEPES, 70 mM NH4Cl, 30 mM KCl, 3.5 mM MgCl2, 0.6 mM spermine, 0.4 mM spermidine cryo-EM vitrification conditions Cryogen ETHANE；Manual blotting & plunge-freezing	Resolution 6.00 Å
7Q4K Erythromycin-stalled Escherichia coli 70S ribosome with streptococcal MsrDL nascent chain Deposited 2021-10-31	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric	Chain BV 1–94（94 aa）	Not recorded	MG MAGNESIUM ION × 172 ERY ERYTHROMYCIN A × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.00 Å
7QG8 Structure of the collided E. coli disome - VemP-stalled 70S ribosome Deposited 2021-12-07	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 57-meric	Chain i 1–94（94 aa）	Not recorded	MG MAGNESIUM ION × 2 K POTASSIUM ION × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.97 Å
7QGH Structure of the E. coli disome - collided 70S ribosome Deposited 2021-12-08	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 58-meric	Chain i 1–94（94 aa）	Not recorded	MG MAGNESIUM ION × 2 K POTASSIUM ION × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 4.48 Å
7QGN Structure of the SmrB-bound E. coli disome - stalled 70S ribosome Deposited 2021-12-09	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 53 PDB declaration: 58-meric	Chain i 1–94（94 aa）	Not recorded	MG MAGNESIUM ION × 2 K POTASSIUM ION × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.37 Å
7QGR Structure of the SmrB-bound E. coli disome - collided 70S ribosome Deposited 2021-12-09	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 53 PDB declaration: 59-meric	Chain i 1–94（94 aa）	Not recorded	MG MAGNESIUM ION × 2 K POTASSIUM ION × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 5.70 Å
7QQ3 Cryo-EM structure of the E.coli 50S ribosomal subunit in complex with the antibiotic Myxovalargin A. Deposited 2022-01-06	Different oligomeric state Different ligand/ion Different experimental conditions	Assembly 1 Protein–RNA Heteromer；Protein × 27 PDB declaration: 29-meric	Chain d 1–94（94 aa）	Not recorded	MG MAGNESIUM ION × 145 ZN ZINC ION × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5；Solutions were made fresh, pH was adjusted with potassium hydroxide at 4 degrees celcius and sterile filtered. cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；2 seconds blotting before plunging.	Resolution 2.10 Å
7SS9 Late translocation intermediate with EF-G partially dissociated (Structure V) Deposited 2021-11-10	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 57-meric	Chain v 1–94（94 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.90 Å
7SSD Mid translocation intermediate with EF-G bound with GDP (Structure IV) Deposited 2021-11-10	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 58-meric	Chain v 1–94（94 aa）	Not recorded	GDP GUANOSINE-5'-DIPHOSPHATE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.30 Å
7SSL Pre translocation intermediate with EF-G bound to GDP and Pi (Structure III) Deposited 2021-11-11	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 58-meric	Chain v 1–94（94 aa）	Not recorded	GDP GUANOSINE-5'-DIPHOSPHATE × 1 PO4 PHOSPHATE ION × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.80 Å
7SSN Pre translocation 70S ribosome with A/P* and P/E tRNA (Structure II-B) Deposited 2021-11-11	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 54-meric	Chain v 1–94（94 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.20 Å
7SSO Pre translocation 70S ribosome with A/A and P/E tRNA (Structure II-A) Deposited 2021-11-11	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 58-meric	Chain v 1–94（94 aa）	Not recorded	FME N-FORMYLMETHIONINE × 1 PRO PROLINE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.20 Å
7SSW Late translocation intermediate with EF-G dissociated (Structure VI) Deposited 2021-11-11	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric	Chain v 1–94（94 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.80 Å
7ST2 Post translocation, non-rotated 70S ribosome with EF-G dissociated (Structure VII) Deposited 2021-11-11	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 58-meric	Chain v 1–94（94 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.90 Å
7ST6 Pre translocation, non-rotated 70S ribosome (Structure I) Deposited 2021-11-12	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 57-meric	Chain v 1–94（94 aa）	Not recorded	PRO PROLINE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.00 Å
7ST7 Pre translocation intermediate stalled with viomycin and bound with EF-G in a GDP and Pi state (Structure III-vio) Deposited 2021-11-12	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 53 PDB declaration: 59-meric	Chain v 1–94（94 aa）	Not recorded	GDP GUANOSINE-5'-DIPHOSPHATE × 1 PO4 PHOSPHATE ION × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.20 Å
7Y7C Structure of the Bacterial Ribosome with human tRNA Asp(G34) and mRNA(GAU) Deposited 2022-06-22	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric	Chain u 1–94（94 aa）	Not recorded	MG MAGNESIUM ION × 283	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6；The Buffer pH was adjusted to 7.6 using KOH. cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.51 Å
7Y7D Structure of the Bacterial Ribosome with human tRNA Asp(Q34) and mRNA(GAU) Deposited 2022-06-22	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric	Chain u 1–94（94 aa）	Not recorded	MG MAGNESIUM ION × 258	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6；The Buffer pH was adjusted to 7.6 using KOH. cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.58 Å
7Y7E Structure of the Bacterial Ribosome with human tRNA Asp(ManQ34) and mRNA(GAU) Deposited 2022-06-22	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric	Chain u 1–94（94 aa）	Not recorded	MG MAGNESIUM ION × 248 MAN alpha-D-mannopyranose × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6；The Buffer pH was adjusted to 7.6 using KOH. cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.41 Å
7Y7F Structure of the Bacterial Ribosome with human tRNA Asp(ManQ34) and mRNA(GAC) Deposited 2022-06-22	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric	Chain u 1–94（94 aa）	Not recorded	MG MAGNESIUM ION × 259 MAN alpha-D-mannopyranose × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6；The Buffer pH was adjusted to 7.6 using KOH. cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.43 Å
7Y7G Structure of the Bacterial Ribosome with human tRNA Tyr(GalQ34) and mRNA(UAU) Deposited 2022-06-22	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric	Chain u 1–94（94 aa）	Not recorded	MG MAGNESIUM ION × 256 GAL beta-D-galactopyranose × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6；The Buffer pH was adjusted to 7.6 using KOH. cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.34 Å
7Y7H Structure of the Bacterial Ribosome with human tRNA Tyr(GalQ34) and mRNA(UAC) Deposited 2022-06-22	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric	Chain u 1–94（94 aa）	Not recorded	MG MAGNESIUM ION × 263 GAL beta-D-galactopyranose × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6；The Buffer pH was adjusted to 7.6 using KOH. cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.51 Å
7YLA Cryo-EM structure of 50S-HflX complex Deposited 2022-07-25	Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 30 PDB declaration: 32-meric	Chain d 1–94（94 aa）	Not recorded	GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 3 NA SODIUM ION × 1 ZN ZINC ION × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.52 Å
7Z20 70S E. coli ribosome with an extended uL23 loop from Candidatus marinimicrobia and a stalled filamin domain 5 nascent chain Deposited 2022-02-25	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 29 PDB declaration: 32-meric	Chain w 1–94（94 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.29 Å
7ZOD 70S E. coli ribosome with an extended uL23 loop from Candidatus marinimicrobia Deposited 2022-04-25	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 28 PDB declaration: 31-meric	Chain w 1–94（94 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.56 Å
7ZP8 70S E. coli ribosome with a stalled filamin domain 5 nascent chain Deposited 2022-04-26	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 29 PDB declaration: 32-meric	Chain w 1–94（94 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.20 Å
7ZQ5 70S E. coli ribosome with truncated uL23 and uL24 loops Deposited 2022-04-29	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 28 PDB declaration: 30-meric	Chain w 1–94（94 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.70 Å
7ZQ6 70S E. coli ribosome with truncated uL23 and uL24 loops and a stalled filamin domain 5 nascent chain Deposited 2022-04-29	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 29 PDB declaration: 32-meric	Chain w 1–94（94 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.75 Å
7ZTA Structure of an Escherichia coli 70S ribosome stalled by Tetracenomycin X during translation of an MAAAPQK(C) peptide Deposited 2022-05-09	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 57-meric	Chain L251 1–94（94 aa）	Not recorded	MG MAGNESIUM ION × 289 K POTASSIUM ION × 115 ZN ZINC ION × 3 OCW Tetracenomycin X × 1 CYS CYSTEINE × 1 GLN GLUTAMINE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE；Waiting 30 sec Blotting 2.5 sec Blot force 5	Resolution 2.70 Å
8A3L Structural insights into the binding of bS1 to the ribosome Deposited 2022-06-08	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric	Chain u 1–94（94 aa）	Not recorded	MG MAGNESIUM ION × 313 ZN ZINC ION × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.42 Å
8AKN Cryo-EM structure of the proline-rich antimicrobial peptide drosocin bound to the terminating ribosome Deposited 2022-07-30	Different oligomeric state Different ligand/ion Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 57-meric	Chain u 1–94（94 aa）	Not recorded	ZN ZINC ION × 2 A2G 2-acetamido-2-deoxy-alpha-D-galactopyranose × 1 MG MAGNESIUM ION × 305 SPM SPERMINE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE-PROPANE	Resolution 2.30 Å
8AM9 Cryo-EM structure of the proline-rich antimicrobial peptide drosocin bound to the elongating ribosome Deposited 2022-08-03	Different oligomeric state Different ligand/ion Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric	Chain u 1–94（94 aa）	Not recorded	ZN ZINC ION × 2 MG MAGNESIUM ION × 293 SPM SPERMINE × 1 A2G 2-acetamido-2-deoxy-alpha-D-galactopyranose × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE-PROPANE	Resolution 2.80 Å
8AP4 Structure of Escherischia coli heat shock protein Hsp15 in complex with ribosomal 50S subunits bearing peptidyl-tRNA Deposited 2022-08-09	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 30 PDB declaration: 33-meric	Chain u 1–94（94 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.46 cryo-EM vitrification conditions Cryogen ETHANE-PROPANE	Resolution 3.00 Å
8AYE E. coli 70S ribosome bound to thermorubin and fMet-tRNA Deposited 2022-09-02	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric	Chain u 1–94（94 aa）	Not recorded	ZN ZINC ION × 2 MG MAGNESIUM ION × 308 T8B Thermorubin × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 1.96 Å
8B0X Translating 70S ribosome in the unrotated state (P and E, tRNAs) Deposited 2022-09-08	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 53-meric	Chain u 1–94（94 aa）	Not recorded	ZN ZINC ION × 1 K POTASSIUM ION × 168 MG MAGNESIUM ION × 361	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 1.55 Å
8B7Y Cryo-EM structure of the E.coli 70S ribosome in complex with the antibiotic Myxovalargin B. Deposited 2022-10-03	Different oligomeric state Different ligand/ion Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 47 PDB declaration: 52-meric	Chain d 1–94（94 aa）	Not recorded	MG MAGNESIUM ION × 237 ZN ZINC ION × 1 FME N-FORMYLMETHIONINE × 1 SPD SPERMIDINE × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE-PROPANE	Resolution 3.00 Å
8BF7 Elongating E. coli 70S ribosome containing deacylated tRNA(iMet) in the P-site and AAA mRNA codon with cognate dipeptidyl-tRNA(Lys) in the A-site Deposited 2022-10-24	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric	Chain S 1–94（94 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5；1x TAKM7 buffer cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.33 Å
8BGE Elongating E. coli 70S ribosome containing acylated tRNA(iMet) in the P-site and AAm6A mRNA codon in the A-site after uncompleted di-peptide formation Deposited 2022-10-27	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 53-meric	Chain S 1–94（94 aa）	Not recorded	FME N-FORMYLMETHIONINE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5；1x TAKM7 buffer cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.11 Å
8BGH Elongating E. coli 70S ribosome containing acylated tRNA(iMet) in the P-site and AAA mRNA codon in the A-site after uncompleted di-peptide formation Deposited 2022-10-27	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 54-meric	Chain S 1–94（94 aa）	Not recorded	FME N-FORMYLMETHIONINE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5；1x TAKM7 buffer cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.88 Å
8BH4 Elongating E. coli 70S ribosome containing deacylated tRNA(iMet) in the P-site and AAm6A mRNA codon with cognate dipeptidyl-tRNA(Lys) in the A-site Deposited 2022-10-29	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric	Chain S 1–94（94 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5；1x TAKM7 buffer cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.62 Å
8BHJ Elongating E. coli 70S ribosome containing deacylated tRNA(iMet) in the P-site and Am6AA mRNA codon with cognate dipeptidyl-tRNA(Lys) in the A-site Deposited 2022-10-31	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric	Chain S 1–94（94 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5；1x TAKM7 buffer cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.81 Å
8BHL Elongating E. coli 70S ribosome containing acylated tRNA(iMet) in the P-site and Am6AA mRNA codon in the A-site after uncompleted di-peptide formation Deposited 2022-10-31	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 53-meric	Chain S 1–94（94 aa）	Not recorded	FME N-FORMYLMETHIONINE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5；1x TAKM7 buffer cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.21 Å
8BHN Elongating E. coli 70S ribosome containing deacylated tRNA(iMet) in the P-site and m6AAA mRNA codon with cognate dipeptidyl-tRNA(Lys) in the A-site Deposited 2022-10-31	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric	Chain S 1–94（94 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5；1x TAKM7 buffer cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.85 Å
8BHP Elongating E. coli 70S ribosome containing acylated tRNA(iMet) in the P-site and m6AAA mRNA codon in the A-site after uncompleted di-peptide formation Deposited 2022-10-31	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 54-meric	Chain S 1–94（94 aa）	Not recorded	FME N-FORMYLMETHIONINE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5；1x TAKM7 buffer cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.37 Å
8BIL Initiation complex of the E. coli 70S ribosome with mRNA containing AAA codon in the A-site. Deposited 2022-11-02	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 54-meric	Chain S 1–94（94 aa）	Not recorded	FME N-FORMYLMETHIONINE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5；1x TAKM7 buffer cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.04 Å
8BIM Initiation complex of the E. coli 70S ribosome with mRNA containing AAm6A codon in the A-site Deposited 2022-11-02	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 54-meric	Chain S 1–94（94 aa）	Not recorded	FME N-FORMYLMETHIONINE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5；1x TAKM7 buffer cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.04 Å
8C8X Cryo-EM captures early ribosome assembly in action Deposited 2023-01-21	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 26 PDB declaration: 28-meric	Chain V 1–94（94 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.93 Å
8C8Y Cryo-EM captures early ribosome assembly in action Deposited 2023-01-21	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 23 PDB declaration: 25-meric	Chain V 1–94（94 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.03 Å
8C8Z Cryo-EM captures early ribosome assembly in action Deposited 2023-01-21	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 22 PDB declaration: 24-meric	Chain V 1–94（94 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.12 Å
8C90 Cryo-EM captures early ribosome assembly in action Deposited 2023-01-21	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 20 PDB declaration: 22-meric	Chain V 1–94（94 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.15 Å
8C94 Cryo-EM captures early ribosome assembly in action Deposited 2023-01-21	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 19 PDB declaration: 21-meric	Chain V 1–94（94 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.80 Å
8C95 Cryo-EM captures early ribosome assembly in action Deposited 2023-01-21	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 14 PDB declaration: hexadecameric	Chain V 1–94（94 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 4.92 Å
8CAM Evernimicin bound to the 50S subunit Deposited 2023-01-24	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 26 PDB declaration: 28-meric	Chain u 1–94（94 aa）	Not recorded	ZN ZINC ION × 1 ACT ACETATE ION × 1 MG MAGNESIUM ION × 246 K POTASSIUM ION × 72 6O1 Evernimicin × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 1.86 Å
8CEU Retapamulin and Capreomycin bound to the 50S subunit Deposited 2023-02-02	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 36 PDB declaration: 38-meric	Chain u 1–94（94 aa）	Not recorded	ZN ZINC ION × 1 G34 Retapamulin × 1 MG MAGNESIUM ION × 220 K POTASSIUM ION × 81	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 1.83 Å
8CGD Clindamycin bound to the 50S subunit Deposited 2023-02-03	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 28 PDB declaration: 30-meric	Chain u 1–94（94 aa）	Not recorded	ZN ZINC ION × 2 ACT ACETATE ION × 1 CLY CLINDAMYCIN × 1 AM2 APRAMYCIN × 1 MG MAGNESIUM ION × 254 K POTASSIUM ION × 85	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 1.98 Å
8CGK Lincomycin and Avilamycin bound to the 50S subunit Deposited 2023-02-05	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 26 PDB declaration: 28-meric	Chain u 1–94（94 aa）	Not recorded	ZN ZINC ION × 1 ACT ACETATE ION × 1 3QB LINCOMYCIN × 1 6UQ (2R,3S,4R,6S)-4-hydroxy-6-{[(2R,3aR,4R,4'R,5'S,6S,6'R,7aR)-4'-hydroxy-6-{[(2S,3R,4R,5S,6R)-3-hydroxy-2-{[(2R,3S,4S,5S,6S)-4-hydroxy-6-({(2R,3aS,3a'R,6S,6'R,7R,7'R,7aR,7a'R)-7'-hydroxy-7'-[(1S)-1-hydroxyethyl]-6'-methyl-7-[(2-methylpropanoyl)oxy]octahydro-4H-2,4'-spirobi[[1,3]dioxolo[4,5-c]pyran]-6-yl}oxy)-5-methoxy-2-(methoxymethyl)tetrahydro-2H-pyran-3-yl]oxy}-5-methoxy-6-methyltetrahydro-2H-pyran-4-yl]oxy}-4,6',7a-trimethyloctahydro-4H-spiro[1,3-dioxolo[4,5-c]pyran-2,2'-pyran]-5'-yl]oxy}-2-methyltetrahydro-2H-pyran-3-yl 3,5-dichloro-4-hydroxy-2-methoxy-6-methylbenzoate (non-preferred name) × 1 MG MAGNESIUM ION × 260 K POTASSIUM ION × 81	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 1.64 Å
8CGV Tiamulin bound to the 50S subunit Deposited 2023-02-06	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 27 PDB declaration: 29-meric	Chain u 1–94（94 aa）	Not recorded	ZN ZINC ION × 1 P8F Pentacycline × 2 MUL TIAMULIN × 1 MG MAGNESIUM ION × 219 K POTASSIUM ION × 83	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 1.66 Å
8HSP E. coli 70S ribosome complexed with tRNA_Ile2 bearing L34 and t6A37 in classical state Deposited 2022-12-20	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric	Chain u 1–94（94 aa）	Not recorded	MG MAGNESIUM ION × 315 K POTASSIUM ION × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6；The Buffer pH was adjusted to 7.6 using KOH. cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.32 Å
8HTZ E. coli 70S ribosome complexed with H. marismortui tRNA_Ile2 bearing agm2C34 in classical state Deposited 2022-12-22	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric	Chain u 1–94（94 aa）	Not recorded	MG MAGNESIUM ION × 317 K POTASSIUM ION × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6；The Buffer pH was adjusted to 7.6 using KOH. cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.40 Å
8HU1 E. coli 70S ribosome complexed with tRNA_Ile2 bearing L34 and ct6A37 in classical state Deposited 2022-12-22	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric	Chain u 1–94（94 aa）	Not recorded	MG MAGNESIUM ION × 314 K POTASSIUM ION × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6；The Buffer pH was adjusted to 7.6 using KOH. cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.69 Å
8IFB Dibekacin-bound E.coli 70S ribosome in the PURE system Deposited 2023-02-17	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric	Chain u 1–94（94 aa）	Not recorded	MG MAGNESIUM ION × 406 SPD SPERMIDINE × 14 84D Dibekacin × 13 SPM SPERMINE × 1 ZN ZINC ION × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.43 Å
8IFC Arbekacin-bound E.coli 70S ribosome in the PURE system Deposited 2023-02-17	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric	Chain u 1–94（94 aa）	Not recorded	MG MAGNESIUM ION × 460 SPD SPERMIDINE × 16 84G Arbekacin × 10 SPM SPERMINE × 1 ZN ZINC ION × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.90 Å
8J1Z The global structure of pre50S related to DbpA in state3 Deposited 2023-04-13	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 25 PDB declaration: 27-meric	Chain u 1–94（94 aa）	Not recorded	MG MAGNESIUM ION × 210	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5；20mM HEPES-KOH,pH7.5;140mM NH4Cl;10mM Mg2+,1mM DTT cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.60 Å
8P16 E167K RF2 on E. coli 70S release complex with UGG (Structure I) Deposited 2023-05-11	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 56-meric	Chain V 1–94（94 aa）	Not recorded	MG MAGNESIUM ION × 330 SPD SPERMIDINE × 13 K POTASSIUM ION × 128 PUT 1,4-DIAMINOBUTANE × 13 SPM SPERMINE × 1 ZN ZINC ION × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5；HEPES-polymix buffer (pH-7.5) cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.77 Å
8P17 E167K RF2 on E. coli 70S release complex with UGG (Structure II) Deposited 2023-05-11	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 56-meric	Chain V 1–94（94 aa）	Not recorded	MG MAGNESIUM ION × 325 SPD SPERMIDINE × 13 K POTASSIUM ION × 128 PUT 1,4-DIAMINOBUTANE × 13 SPM SPERMINE × 1 ZN ZINC ION × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5；HEPES-polymix buffer (pH-7.5) cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.78 Å
8P18 E167K RF2 on E. coli 70S release complex with UGG (Structure III) Deposited 2023-05-11	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 56-meric	Chain V 1–94（94 aa）	Not recorded	MG MAGNESIUM ION × 321 SPD SPERMIDINE × 13 K POTASSIUM ION × 127 PUT 1,4-DIAMINOBUTANE × 13 SPM SPERMINE × 1 ZN ZINC ION × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5；HEPES-polymix buffer (pH-7.5) cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.77 Å
8PEG Escherichia coli paused disome complex (queueing 70S non-rotated closed PRE state) Deposited 2023-06-13	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 54 PDB declaration: 61-meric	Chain y 1–94（94 aa）	Not recorded	ZN ZINC ION × 2 MG MAGNESIUM ION × 260 ATP ADENOSINE-5'-TRIPHOSPHATE × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6 cryo-EM vitrification conditions Cryogen ETHANE；Withdrawn samples were spotted directly onto freshly glow-discharged holey carbon grids, blotted for 1-2 s, and flash frozen in liquid ethane using a Vitrobot Mark IV plunger (ThermoFisher Scientific) after a wait time of 40 s at 4 degrees Celcius.	Resolution 3.30 Å
8PHJ cA4-bound Cami1 in complex with 70S ribosome Deposited 2023-06-20	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 54 PDB declaration: 61-meric	Chain u 1–94（94 aa）	Not recorded	ZN ZINC ION × 2 MG MAGNESIUM ION × 241	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.67 Å
8PKL Escherichia coli paused disome complex (leading 70S non-rotated closed PRE state) Deposited 2023-06-26	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 53 PDB declaration: 60-meric	Chain y 1–94（94 aa）	Not recorded	ZN ZINC ION × 2 PUT 1,4-DIAMINOBUTANE × 2 SPD SPERMIDINE × 1 MG MAGNESIUM ION × 287 ALA ALANINE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6 cryo-EM vitrification conditions Cryogen ETHANE；Withdrawn samples were spotted directly onto freshly glow-discharged holey carbon grids, blotted for 1-2 s, and flash frozen in liquid ethane using a Vitrobot Mark IV plunger (ThermoFisher Scientific) after a wait time of 40 s at 4 degrees Celcius.	Resolution 3.09 Å
8PVA Structure of bacterial ribosome determined by cryoEM at 100 keV Deposited 2023-07-17	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric	Chain u 1–94（94 aa）	Not recorded	ZN ZINC ION × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 4.50 Å
8QBT E. coli ApdP-stalled ribosomal complex Deposited 2023-08-25	Different oligomeric state Different ligand/ion Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 45 PDB declaration: 52-meric	Chain V 1–94（94 aa）	Not recorded	MG MAGNESIUM ION × 248 K POTASSIUM ION × 127 PRO PROLINE × 1 ZN ZINC ION × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE-PROPANE	Resolution 2.20 Å
8QK7 E167K RF2 on E. coli 70S release complex with UAA Deposited 2023-09-14	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 56-meric	Chain V 1–94（94 aa）	Not recorded	MG MAGNESIUM ION × 332 PUT 1,4-DIAMINOBUTANE × 21 K POTASSIUM ION × 126 SPD SPERMIDINE × 10 SPM SPERMINE × 2 ZN ZINC ION × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5；HEPES-polymix buffer (pH-7.5) cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.77 Å
8QOA Structure of SecM-stalled Escherichia coli 70S ribosome Deposited 2023-09-28	Different oligomeric state Different ligand/ion Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 57-meric	Chain u 1–94（94 aa）	Not recorded	ZN ZINC ION × 2 K POTASSIUM ION × 132 MG MAGNESIUM ION × 288 PRO PROLINE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE-PROPANE	Resolution 2.00 Å
8R6C 70S Escherichia coli ribosome with Paenilamicin B2 bound with A- and P-site tRNA. Deposited 2023-11-22	Different oligomeric state Different ligand/ion Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 57-meric	Chain u 1–94（94 aa）	Not recorded	ZN ZINC ION × 2 MG MAGNESIUM ION × 308 K POTASSIUM ION × 3	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE-PROPANE	Resolution 2.20 Å
8R8M 70S Escherichia coli ribosome with Paenilamicin B2 bound with hybrid A/P- and hybrid P/E-tRNA. Deposited 2023-11-29	Different oligomeric state Different ligand/ion Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric	Chain u 1–94（94 aa）	Not recorded	ZN ZINC ION × 2 MG MAGNESIUM ION × 307 K POTASSIUM ION × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE-PROPANE	Resolution 2.40 Å
8RPY Escherichia coli 50S subunit in complex with the antimicrobial peptide Api137 Deposited 2024-01-17	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 31 PDB declaration: 33-meric	Chain V 1–94（94 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.64 Å
8RPZ Escherichia coli 50S subunit in complex with the antimicrobial peptide Api88 - conformation I Deposited 2024-01-17	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 32 PDB declaration: 34-meric	Chain V 1–94（94 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.44 Å
8RQ0 Escherichia coli 50S subunit in complex with the antimicrobial peptide Api88 - conformation II Deposited 2024-01-17	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 32 PDB declaration: 34-meric	Chain V 1–94（94 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.44 Å
8RQ2 Escherichia coli 50S subunit in complex with the antimicrobial peptide Api88 - conformation III Deposited 2024-01-17	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 32 PDB declaration: 34-meric	Chain V 1–94（94 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.44 Å
8URM E. coli 70S ribosome with unmodified tRNAPro(GGG) bound to slippery P-site CCC-C codon and tRNAVal(UAC) in the A site Deposited 2023-10-26	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric	Chain V 1–94（94 aa）	Not recorded	MG MAGNESIUM ION × 395	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.00 Å
8URY Escherichia coli transcription-translation coupled complex class B (TTC-B) containing RfaH bound to ops signal, NusA, mRNA with a 30 nt long spacer, and fMet-tRNA in E-site and P-site of the ribosome Deposited 2023-10-27	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Other combination Heteromer；Protein × 60 PDB declaration: 68-meric	Chain 4 1–94（94 aa）	Not recorded	MG MAGNESIUM ION × 1 ZN ZINC ION × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.10 Å
8UTJ E. coli 70S ribosome with unmodified lys-tRNAPro(GGG) bound to slippery P-site CCC-C codon in the 0 frame Deposited 2023-10-31	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric	Chain V 1–94（94 aa）	Not recorded	MG MAGNESIUM ION × 530 LYS LYSINE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.50 Å
8UX8 E. coli 70S ribosome with unmodified Lys-tRNAPro(GGG) bound to slippery P-site CCC-C codon in the +1 mRNA reading frame Deposited 2023-11-09	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric	Chain V 1–94（94 aa）	Not recorded	MG MAGNESIUM ION × 395 LYS LYSINE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.60 Å
8V03 E. coli 70S ribosome with unmodified P/E-tRNAPro(GGG) bound to slippery P-site CCC-C codon Deposited 2023-11-16	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 53-meric	Chain V 1–94（94 aa）	Not recorded	MG MAGNESIUM ION × 281	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 4.00 Å
8VOS Escherichia coli transcription-translation loosely coupled complex (TTC-LC) containing mRNA with a 60 nt long spacer, NusG, NusA, and fMet-tRNAs in E-site and P-site Deposited 2024-01-15	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Other combination Heteromer；Protein × 60 PDB declaration: 68-meric	Chain 4 1–94（94 aa）	Not recorded	MG MAGNESIUM ION × 1 ZN ZINC ION × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 4.60 Å
8W51 Structure of YchF(H114A) on E.coli 50S ribosomal subunit Deposited 2023-08-25	Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 30 PDB declaration: 32-meric	Chain W 1–94（94 aa）	Not recorded	MG MAGNESIUM ION × 104 ZN ZINC ION × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.40 Å
8Y5K E.coli transcription translation coupling complex in TTC-A state 2 containing mRNA with 27-mer spacer, NusG, NusA, fMet-tRNA(iMet), Phe-tRNA(Phe), and viomycin Deposited 2024-01-31	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Other combination Heteromer；Protein × 59 PDB declaration: 67-meric	Chain v 1–94（94 aa）	Not recorded	MG MAGNESIUM ION × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 PO4 PHOSPHATE ION × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 6.90 Å
8Y5L E.coli transcription translation coupling complex in TTC-B state 1 (subclass1) containing mRNA with 30-mer spacer, NusG, NusA, fMet-tRNA(iMet), Phe-tRNA(Phe), and viomycin Deposited 2024-01-31	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Other combination Heteromer；Protein × 58 PDB declaration: 67-meric	Chain v 1–94（94 aa）	Not recorded	MG MAGNESIUM ION × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.70 Å
8Y5M E.coli transcription translation coupling complex in TTC-B state 2 containing mRNA with 27-mer spacer, NusG, NusA, fMet-tRNA(iMet), Phe-tRNA(Phe), and viomycin Deposited 2024-01-31	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Other combination Heteromer；Protein × 60 PDB declaration: 68-meric	Chain v 1–94（94 aa）	Not recorded	MG MAGNESIUM ION × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 PO4 PHOSPHATE ION × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 4.50 Å
8Y5N E.coli transcription translation coupling complex in TTC-A state 3 containing mRNA with 21-mer spacer, NusG, NusA, fMet-tRNA(iMet), Phe-tRNA(Phe), and viomycin Deposited 2024-01-31	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Other combination Heteromer；Protein × 59 PDB declaration: 67-meric	Chain v 1–94（94 aa）	Not recorded	GDP GUANOSINE-5'-DIPHOSPHATE × 1 PO4 PHOSPHATE ION × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 4.50 Å
8Y5O E.coli transcription translation coupling complex in TTC-B state 3 (subclass1) containing mRNA with 30-mer spacer, NusG, NusA, fMet-tRNA(iMet), Phe-tRNA(Phe), and viomycin Deposited 2024-01-31	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Other combination Heteromer；Protein × 60 PDB declaration: 68-meric	Chain v 1–94（94 aa）	Not recorded	MG MAGNESIUM ION × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 PO4 PHOSPHATE ION × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 4.20 Å
8Y5P E.coli transcription translation coupling complex in TTC-B state 4 (subclass 1) containing mRNA with 24-mer spacer, NusG, NusA, fMet-tRNA(iMet), Phe-tRNA(Phe), and viomycin Deposited 2024-01-31	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Other combination Heteromer；Protein × 60 PDB declaration: 67-meric	Chain v 1–94（94 aa）	Not recorded	MG MAGNESIUM ION × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 PO4 PHOSPHATE ION × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 6.50 Å
8Y5Q E.coli transcription translation coupling complex in TTC-B state 4 (subclass 2) containing mRNA with 27-mer spacer, NusG, NusA, fMet-tRNA(iMet), Phe-tRNA(Phe), and GDPCP Deposited 2024-01-31	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Other combination Heteromer；Protein × 59 PDB declaration: 66-meric	Chain v 1–94（94 aa）	Not recorded	MG MAGNESIUM ION × 1 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.90 Å
8Y5R E.coli Transcription translation coupling complex in TTC-B state 5 (subclass 1) containing mRNA with 27-mer spacer, NusG, NusA, fMet-tRNA(iMet), Phe-tRNA(Phe), and fusidic acid Deposited 2024-01-31	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Other combination Heteromer；Protein × 58 PDB declaration: 66-meric	Chain v 1–94（94 aa）	Not recorded	MG MAGNESIUM ION × 1 FUA FUSIDIC ACID × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 4.50 Å
8Y5S E.coli Transcription translation coupling complex in TTC-B state 5 (subclass 2) containing mRNA with 27-mer spacer, NusG, NusA, fMet-tRNA(iMet), Phe-tRNA(Phe), and GDPCP Deposited 2024-01-31	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Other combination Heteromer；Protein × 58 PDB declaration: 66-meric	Chain v 1–94（94 aa）	Not recorded	MG MAGNESIUM ION × 1 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 4.50 Å
8Y5T E.coli Transcription translation coupling complex in TTC-B state 5 (subclass 3) containing mRNA with 27-mer spacer, NusG, NusA, fMet-tRNA(iMet), Phe-tRNA(Phe), and fusidic acid Deposited 2024-01-31	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Other combination Heteromer；Protein × 58 PDB declaration: 66-meric	Chain v 1–94（94 aa）	Not recorded	MG MAGNESIUM ION × 1 FUA FUSIDIC ACID × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 4.40 Å
8YDE E.coli transcription translation coupling complex in TTC-B state 1 (subclass 3) containing mRNA with 39-mer spacer, NusG, NusA, fMet-tRNA(iMet), Phe-tRNA(Phe), and viomycin Deposited 2024-02-20	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Other combination Heteromer；Protein × 58 PDB declaration: 67-meric	Chain v 1–94（94 aa）	Not recorded	MG MAGNESIUM ION × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 5.00 Å
8YDF E.coli transcription translation coupling complex in TTC-B state 1 (subclass 2) containing mRNA with 39-mer spacer, NusG, NusA, fMet-tRNA(iMet), Phe-tRNA(Phe), and viomycin Deposited 2024-02-20	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Other combination Heteromer；Protein × 58 PDB declaration: 67-meric	Chain v 1–94（94 aa）	Not recorded	MG MAGNESIUM ION × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 4.60 Å
8YDG E.coli transcription translation coupling complex in TTC-B state 3 (subclass2) containing mRNA with 39-mer spacer, NusG, NusA, fMet-tRNA(iMet), Phe-tRNA(Phe), and viomycin Deposited 2024-02-20	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Other combination Heteromer；Protein × 60 PDB declaration: 68-meric	Chain v 1–94（94 aa）	Not recorded	MG MAGNESIUM ION × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 PO4 PHOSPHATE ION × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 5.10 Å
8YDH E.coli transcription translation coupling complex in TTC-P state 1 (subclass1) containing mRNA with 39-mer spacer, NusG, NusA, fMet-tRNA(iMet), Phe-tRNA(Phe), and viomycin Deposited 2024-02-20	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Other combination Heteromer；Protein × 58 PDB declaration: 67-meric	Chain v 1–94（94 aa）	Not recorded	MG MAGNESIUM ION × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 4.55 Å
8YDI E.coli transcription translation coupling complex in TTC-P state 1 (subclass 2) containing mRNA with 39-mer spacer, NusG, NusA, fMet-tRNA(iMet), Phe-tRNA(Phe), and viomycin Deposited 2024-02-20	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Other combination Heteromer；Protein × 58 PDB declaration: 67-meric	Chain v 1–94（94 aa）	Not recorded	MG MAGNESIUM ION × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 4.60 Å
8YDJ E.coli transcription translation coupling complex in TTC-P containing mRNA with 39-mer spacer, NusG, NusA, fMet-tRNA(iMet), Phe-tRNA(Phe), and viomycin Deposited 2024-02-20	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Other combination Heteromer；Protein × 60 PDB declaration: 68-meric	Chain v 1–94（94 aa）	Not recorded	MG MAGNESIUM ION × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 PO4 PHOSPHATE ION × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 5.10 Å
8YUO E. coli 70S ribosome complexed with P. putida tRNAIle2 at the A-site and P-site Deposited 2024-03-27	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric	Chain u 1–94（94 aa）	Not recorded	MG MAGNESIUM ION × 309	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6；The Buffer pH was adjusted to 7.6 using KOH. cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.25 Å
8YUP E. coli 70S ribosome complexed with P. putida tRNAIle2 and A4 mRNA Deposited 2024-03-28	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric	Chain u 1–94（94 aa）	Not recorded	MG MAGNESIUM ION × 309	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6；The Buffer pH was adjusted to 7.6 using KOH. cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.39 Å
8YUQ E. coli 70S ribosome complexed with P. putida tRNAIle2 and dA4 mRNA Deposited 2024-03-27	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric	Chain u 1–94（94 aa）	Not recorded	MG MAGNESIUM ION × 309	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6；The Buffer pH was adjusted to 7.6 using KOH. cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.41 Å
8YUR E. coli 70S ribosome complexed with P. putida tRNAIle2 and Am4 mRNA Deposited 2024-03-27	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric	Chain u 1–94（94 aa）	Not recorded	MG MAGNESIUM ION × 309	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6；The Buffer pH was adjusted to 7.6 using KOH. cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.47 Å
8YUS E. coli 70S ribosome complexed with P.putida tRNAIle2 and A(F)4 mRNA Deposited 2024-03-27	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric	Chain u 1–94（94 aa）	Not recorded	MG MAGNESIUM ION × 309	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6；The Buffer pH was adjusted to 7.6 using KOH. cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.43 Å
8ZEJ Structure of E.coli ribosome in complex with an engineered arrest peptide Deposited 2024-05-06	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric	Chain w 1–94（94 aa）	Not recorded	ZN ZINC ION × 1 PRO PROLINE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6；20 mM HEPES (pH 7.6), 140 mM NH4Cl, 25 mM MgCl2, 2 mM DTT cryo-EM vitrification conditions Cryogen NITROGEN	Resolution 2.69 Å
8ZFF Structure of the Bacterial Ribosome without hypoxia-induced rRNA modifications Deposited 2024-05-07	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 54-meric	Chain u 1–94（94 aa）	Not recorded	MG MAGNESIUM ION × 457	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6；The Buffer pH was adjusted to 7.6 using KOH. cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.59 Å
8ZFG Structure of the Bacterial Ribosome with hypoxia-induced rRNA modifications Deposited 2024-05-07	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 54-meric	Chain u 1–94（94 aa）	Not recorded	MG MAGNESIUM ION × 471	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6；The Buffer pH was adjusted to 7.6 using KOH. cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.68 Å
8ZFI Structure of E.coli ribosome in complex with an engineered arrest peptide and trigger factor Deposited 2024-05-07	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 57-meric	Chain w 1–94（94 aa）	Not recorded	ZN ZINC ION × 1 PRO PROLINE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6；20 mM HEPES (pH 7.6), 140 mM NH4Cl, 25 mM MgCl2, 2 mM DTT cryo-EM vitrification conditions Cryogen NITROGEN	Resolution 2.90 Å
8ZTU 70S ribosome arrested by PepNL Deposited 2024-06-07	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric	Chain u 1–94（94 aa）	Not recorded	MG MAGNESIUM ION × 300 K POTASSIUM ION × 47 SPD SPERMIDINE × 7 PUT 1,4-DIAMINOBUTANE × 1 ZN ZINC ION × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.90 Å
8ZTV 70S ribosome arrested by PepNL with RF2 Deposited 2024-06-07	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 57-meric	Chain u 1–94（94 aa）	Not recorded	MG MAGNESIUM ION × 301 K POTASSIUM ION × 47 SPD SPERMIDINE × 7 PUT 1,4-DIAMINOBUTANE × 1 ZN ZINC ION × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.90 Å
9AX7 70S initiation complex (tRNA-fMet M1 + CUG start codon) Deposited 2024-03-06	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 54-meric	Chain u 1–94（94 aa）	Not recorded	ZN ZINC ION × 2 MG MAGNESIUM ION × 309 IAS BETA-L-ASPARTIC ACID × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.63 Å
9CG5 70S initiation complex (tRNA-fMet M1 + UUG start codon) Deposited 2024-06-28	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 54-meric	Chain u 1–94（94 aa）	Not recorded	ZN ZINC ION × 2 MG MAGNESIUM ION × 309 IAS BETA-L-ASPARTIC ACID × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.59 Å
9CG6 70S initiation complex (tRNA-fMet M1 + GUG start codon) Deposited 2024-06-28	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 54-meric	Chain u 1–94（94 aa）	Not recorded	ZN ZINC ION × 2 MG MAGNESIUM ION × 309 IAS BETA-L-ASPARTIC ACID × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.61 Å
9CG7 70S initiation complex (tRNA-fMet M1 + AUG start codon) Deposited 2024-06-28	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 54-meric	Chain u 1–94（94 aa）	Not recorded	ZN ZINC ION × 2 MG MAGNESIUM ION × 309 IAS BETA-L-ASPARTIC ACID × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.75 Å
9D89 E. coli 50S ribosomal subunit in complex with PrAMP rumicidin-2 (focused refinement) Deposited 2024-08-19	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 29 PDB declaration: 31-meric	Chain u 1–94（94 aa）	Not recorded	ZN ZINC ION × 1 MG MAGNESIUM ION × 204 K POTASSIUM ION × 93	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 1.95 Å
9FBV 70S Escherichia coli ribosome with P-site initiatior tRNA. Deposited 2024-05-14	Different oligomeric state Different ligand/ion Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 54-meric	Chain u 1–94（94 aa）	Not recorded	ZN ZINC ION × 2 MG MAGNESIUM ION × 306 K POTASSIUM ION × 3	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE-PROPANE	Resolution 2.40 Å
9GFT Structure of the HrpA-bound E. coli disome, Class I Deposited 2024-08-12	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 105 PDB declaration: 116-meric	Chain Aq 1–94（94 aa） Chain i 1–94（94 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.10 Å
9GGR Structure of the HrpA-bound E. coli disome, Class II Deposited 2024-08-14	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 106 PDB declaration: 117-meric	Chain Aq 1–94（94 aa） Chain i 1–94（94 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.20 Å
9GHA Fusidic acid-locked Escherichia coli 70S ribosome with Staphylococcus aureus EF-G and a tRNA in pe/E chimeric state (CHI) Deposited 2024-08-15	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric	Chain u 1–94（94 aa）	Not recorded	ZN ZINC ION × 2 K POTASSIUM ION × 94 MG MAGNESIUM ION × 271 GDP GUANOSINE-5'-DIPHOSPHATE × 1 FUA FUSIDIC ACID × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.24 Å
9GHB Fusidic acid-locked Escherichia coli 70S ribosome with Staphylococcus aureus EF-G in post-translocational state (POST) Deposited 2024-08-15	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric	Chain u 1–94（94 aa）	Not recorded	ZN ZINC ION × 2 K POTASSIUM ION × 111 MG MAGNESIUM ION × 303 GDP GUANOSINE-5'-DIPHOSPHATE × 1 FUA FUSIDIC ACID × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.21 Å
9GHC Pre-release fusidic acid-locked Escherichia coli 70S ribosome with Staphylococus aureus EF-G and FusB (FusB-EF-G-70S) Deposited 2024-08-15	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 56-meric	Chain u 1–94（94 aa）	Not recorded	ZN ZINC ION × 3 K POTASSIUM ION × 109 MG MAGNESIUM ION × 301 GDP GUANOSINE-5'-DIPHOSPHATE × 1 FUA FUSIDIC ACID × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.79 Å
9GHD Escherichia coli 70S ribosome in complex with Staphylococcus aureus FusB-EF-G (FusB-EF-G-70S*) Deposited 2024-08-15	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 56-meric	Chain u 1–94（94 aa）	Not recorded	ZN ZINC ION × 2 K POTASSIUM ION × 112 MG MAGNESIUM ION × 303	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.41 Å
9GHE Staphylococcus aureus FusB bound to the small subunit of the Escherichia coli 70S ribosome (FusB-70S:SSU) Deposited 2024-08-15	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric	Chain u 1–94（94 aa）	Not recorded	ZN ZINC ION × 3 K POTASSIUM ION × 112 MG MAGNESIUM ION × 303	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 1.87 Å
9GHF Staphylococcus aureus FusB bound to the large subunit of the Escherichia coli 70S ribosome (FusB-70S:LSU) Deposited 2024-08-15	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric	Chain u 1–94（94 aa）	Not recorded	ZN ZINC ION × 3 K POTASSIUM ION × 112 MG MAGNESIUM ION × 303	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.40 Å
9GXX 70S ribosome with doublet-decoding tRNASer3 bound to A-site GCA codon Deposited 2024-10-01	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric	Chain u 1–94（94 aa）	Not recorded	MG MAGNESIUM ION × 288 SPD SPERMIDINE × 7 PUT 1,4-DIAMINOBUTANE × 7 SPM SPERMINE × 1 ZN ZINC ION × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5；HEPES-polymix buffer (pH-7.5) cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.61 Å
9H0L 70S ribosome with cognate tRNASer3 bound to A-site AGC codon Deposited 2024-10-08	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric	Chain u 1–94（94 aa）	Not recorded	MG MAGNESIUM ION × 314 SPD SPERMIDINE × 6 SPM SPERMINE × 1 PUT 1,4-DIAMINOBUTANE × 6 ZN ZINC ION × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5；HEPES-polymix buffer (pH-7.5) cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.49 Å
9H3P 50S subunit precursor C-CP_(L22)- Deposited 2024-10-17	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 20 PDB declaration: 22-meric	Chain V 1–94（94 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 7.06 Å
9H3Q 50S subunit precursor C-CP_YjgA_(L22)-~H61 Deposited 2024-10-17	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 21 PDB declaration: 23-meric	Chain V 1–94（94 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 4.02 Å
9H3R 50S subunit precursor C-CP_YjgA_(L22)- Deposited 2024-10-17	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 23 PDB declaration: 25-meric	Chain V 1–94（94 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 4.12 Å
9H3S 50S subunit precursor C-CP_YjgA_L22 Deposited 2024-10-17	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 25 PDB declaration: 27-meric	Chain V 1–94（94 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 4.16 Å
9H3V 50S subunit precursor C-CP_L2-L28 Deposited 2024-10-17	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 23 PDB declaration: 25-meric	Chain V 1–94（94 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.55 Å
9H3W 50S subunit precursor C-CP_H68 Deposited 2024-10-17	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 23 PDB declaration: 25-meric	Chain V 1–94（94 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 5.38 Å
9H3X 50S subunit precursor C-CP_H68_L35 Deposited 2024-10-17	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 25 PDB declaration: 27-meric	Chain V 1–94（94 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 4.12 Å
9H3Y 50S subunit precursor 50S_(L16)- Deposited 2024-10-17	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 26 PDB declaration: 28-meric	Chain V 1–94（94 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.09 Å
9H3Z mature 50S subunit Deposited 2024-10-17	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 28 PDB declaration: 30-meric	Chain V 1–94（94 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.98 Å
9HA4 Pooled 50S subunit C-CP_(L22)- precursor states supplemented with Api137 Deposited 2024-11-01	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 19 PDB declaration: 21-meric	Chain V 1–94（94 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 4.26 Å
9HA6 mature 50S subunit supplemented with Api137 Deposited 2024-11-01	Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 30 PDB declaration: 32-meric	Chain V 1–94（94 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.08 Å
9HA7 Pooled 50S subunit C-CP_(L22)-~H61 precursor states supplemented with Api137 Deposited 2024-11-01	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 17 PDB declaration: 19-meric	Chain V 1–94（94 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 4.37 Å
9HUQ Structure of WT E.coli ribosome with complexed filament nascent chain at length 47, with P-site tRNA Deposited 2024-12-23	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 29 PDB declaration: 32-meric	Chain w 1–94（94 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.49 Å
9HUS Structure of WT E.coli ribosome with complexed filament nascent chain at length 31, with P-site tRNAs Deposited 2024-12-23	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 29 PDB declaration: 32-meric	Chain w 1–94（94 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.40 Å
9HY6 Structure of E.coli ribosome with nascent chain at linker length of 31 amino acids, with mRNA, P-site and A-site tRNAs Deposited 2025-01-09	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 29 PDB declaration: 34-meric	Chain w 1–94（94 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.60 Å
9HYM Structure of WT E.coli ribosome with complexed filament nascent chain at length 34, with mRNA, P-site and A-site tRNAs, and mRNA Deposited 2025-01-10	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 29 PDB declaration: 34-meric	Chain w 1–94（94 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.20 Å
9I3L Structure of E.coli ribosome with filamin mutant Y719E nascent chain at linker length of 47 amino acids, with tRNA Deposited 2025-01-23	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 29 PDB declaration: 32-meric	Chain w 1–94（94 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.10 Å
9IOT Cryo-EM structure of Escherichia coli hibernating ribosome with RNase I mutant Deposited 2024-07-09	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 53 PDB declaration: 56-meric	Chain X 1–94（94 aa）	Not recorded	MG MAGNESIUM ION × 475 CA CALCIUM ION × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6；20 mM HEPES-KOH (pH 7.6), 10 mM Mg(OAc)2, 30 mM NH4Cl, and 6 mM 2-mercaptoethanol cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.70 Å
9JMK 50S Ribosomal Subunit precursor state III Deposited 2024-09-20	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 26 PDB declaration: 28-meric	Chain V 1–94（94 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 4.00 Å
9JPM Structure of the Bacterial Ribosome with human tRNA Lys(mcm5s2U34) and mRNA(AAA) Deposited 2024-09-26	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric	Chain u 1–94（94 aa）	Not recorded	MG MAGNESIUM ION × 391 ZN ZINC ION × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6；The Buffer pH was adjusted to 7.6 using KOH. cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.43 Å
9JPO Structure of the Bacterial Ribosome with human tRNA Lys(mcm5h2U34) and mRNA(AAA) Deposited 2024-09-26	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric	Chain u 1–94（94 aa）	Not recorded	MG MAGNESIUM ION × 448 ZN ZINC ION × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6；The Buffer pH was adjusted to 7.6 using KOH. cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.18 Å
9JPP Structure of the Bacterial Ribosome with human tRNA Lys(mcm5s2U34) and mRNA(AAG) Deposited 2024-09-26	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric	Chain u 1–94（94 aa）	Not recorded	MG MAGNESIUM ION × 443 ZN ZINC ION × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6；The Buffer pH was adjusted to 7.6 using KOH. cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.26 Å
9JQ2 Structure of the Bacterial Ribosome with human tRNA Lys(mcm5h2U34) and mRNA(AAG) Deposited 2024-09-27	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric	Chain u 1–94（94 aa）	Not recorded	MG MAGNESIUM ION × 438 ZN ZINC ION × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6；The Buffer pH was adjusted to 7.6 using KOH. cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.61 Å
9MKK Structure of arbekacin bound Escherichia coli 70S ribosome Deposited 2024-12-17	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 47 PDB declaration: 52-meric	Chain u 1–94（94 aa）	Not recorded	84G Arbekacin × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.20 Å
9MOR Damaged 70S ribosome with PrfH bound Deposited 2024-12-27	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 58-meric	Chain V 1–94（94 aa）	Not recorded	MG MAGNESIUM ION × 439 ZN ZINC ION × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.65 Å
9MQ4 Damaged 70S ribosome with PrfH bound Deposited 2025-01-01	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 58-meric	Chain V 1–94（94 aa）	Not recorded	MG MAGNESIUM ION × 439 ZN ZINC ION × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.78 Å
9N2B Impacts of ribosomal RNA sequence variation on gene expression and phenotype: Cryo-EM structure of the rrsB ribosome (BBB-70S) Deposited 2025-01-28	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 54-meric	Chain LY 1–94（94 aa）	Not recorded	PUT 1,4-DIAMINOBUTANE × 9 SPD SPERMIDINE × 5 MG MAGNESIUM ION × 276 K POTASSIUM ION × 56 ZN ZINC ION × 3 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 FME N-FORMYLMETHIONINE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.20 Å
9N2C Impacts of ribosomal RNA sequence variation on gene expression and phenotype: Cryo-EM structure of the rrsH ribosome (HBB-70S) Deposited 2025-01-28	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 54-meric	Chain LY 1–94（94 aa）	Not recorded	PUT 1,4-DIAMINOBUTANE × 10 SPD SPERMIDINE × 5 MG MAGNESIUM ION × 283 K POTASSIUM ION × 49 ZN ZINC ION × 3 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 FME N-FORMYLMETHIONINE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.40 Å
9NJF E. coli pre-elongation complex without an A-site tRNA with EQ2-YbiT in Non-hydrolytic 1/PtIM(a) conformation Deposited 2025-02-27	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 53 PDB declaration: 58-meric	Chain 25 1–94（94 aa）	Not recorded	MG MAGNESIUM ION × 124 ZN ZINC ION × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 NA SODIUM ION × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.40 Å
9NJV E. coli 70S initiation complex (bL33 absent) Deposited 2025-02-28	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 53-meric	Chain 25 1–94（94 aa）	Not recorded	MG MAGNESIUM ION × 152 FME N-FORMYLMETHIONINE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.10 Å
9NKL E. coli 70S initiation complex Deposited 2025-03-01	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 54-meric	Chain 25 1–94（94 aa）	Not recorded	MG MAGNESIUM ION × 161	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.30 Å
9NL5 E. coli pre-elongation complex without an A-site tRNA with EQ2-EttA in Hydrolytic 1 conformation Deposited 2025-03-02	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 56-meric	Chain 25 1–94（94 aa）	Not recorded	MG MAGNESIUM ION × 125 ZN ZINC ION × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 NA SODIUM ION × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.00 Å
9NL6 E. coli initiation complex with EQ2-YbiT in Non-hydrolytic 1/PtIM(a) conformation Deposited 2025-03-02	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric	Chain 25 1–94（94 aa）	Not recorded	MG MAGNESIUM ION × 252 FME N-FORMYLMETHIONINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 NA SODIUM ION × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.20 Å
9NL7 E. coli initiation complex with EQ2-YbiT in Non-hydrolytic 2/PtIM(b) conformation Deposited 2025-03-02	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 56-meric	Chain 25 1–94（94 aa）	Not recorded	MG MAGNESIUM ION × 250 FME N-FORMYLMETHIONINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 NA SODIUM ION × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.30 Å
9NLB E. coli initiation complex with EQ2-EttA in Hydrolytic 2 conformation Deposited 2025-03-03	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 56-meric	Chain 25 1–94（94 aa）	Not recorded	MG MAGNESIUM ION × 279 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 NA SODIUM ION × 2 FME N-FORMYLMETHIONINE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.20 Å
9NLE E. coli initiation complex with EQ2-EttA in Hydrolytic 1 conformation Deposited 2025-03-03	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 56-meric	Chain 25 1–94（94 aa）	Not recorded	MG MAGNESIUM ION × 298 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 NA SODIUM ION × 2 FME N-FORMYLMETHIONINE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.20 Å
9NLF E. coli initiation complex with EQ2-YbiT in Hydrolytic 2/PtIM(a) conformation Deposited 2025-03-03	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 56-meric	Chain 25 1–94（94 aa）	Not recorded	MG MAGNESIUM ION × 245 FME N-FORMYLMETHIONINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 NA SODIUM ION × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.20 Å
9NLH E.coli Initiation complex with YheS-EQ2 Deposited 2025-03-03	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric	Chain 25 1–94（94 aa）	Not recorded	MG MAGNESIUM ION × 263 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 NA SODIUM ION × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.10 Å
9NLJ E.coli Initiation complex with Uup-EQ2 Deposited 2025-03-03	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 56-meric	Chain 25 1–94（94 aa）	Not recorded	MG MAGNESIUM ION × 277 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 NA SODIUM ION × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.10 Å
9NLQ E. coli initiation complex with EQ2-YbiT in Hydrolytic 1/PtIM(a) conformation Deposited 2025-03-03	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 56-meric	Chain 25 1–94（94 aa）	Not recorded	MG MAGNESIUM ION × 241 FME N-FORMYLMETHIONINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 NA SODIUM ION × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.30 Å
9NLS E. coli initiation complex with EQ2-YbiT in Intermediate/PtIM(b) conformation Deposited 2025-03-03	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 56-meric	Chain 25 1–94（94 aa）	Not recorded	MG MAGNESIUM ION × 248 ZN ZINC ION × 1 FME N-FORMYLMETHIONINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 NA SODIUM ION × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.30 Å
9O2X Structure of WT E.coli ribosome 70S subunit with complexed with mRNA, P-site fMet-NH-tRNAfMet and A-site (S)-betahydroxyBocK charged NH-tRNAPyl Deposited 2025-04-04	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric	Chain u 1–94（94 aa）	Not recorded	ZN ZINC ION × 2 MG MAGNESIUM ION × 320 FME N-FORMYLMETHIONINE × 1 K POTASSIUM ION × 10 PAR PAROMOMYCIN × 1 SPD SPERMIDINE × 14 SPM SPERMINE × 1 A1B71 (2S)-6-[(tert-butoxycarbonyl)amino]-2-(hydroxymethyl)hexanoic acid × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.29 Å
9O2Y Structure of WT E.coli ribosome 70S subunit with complexed with mRNA, P-site fMet-NH-tRNAfMet and A-site (R) beta-2-hydroxy-BocLysine acid charged NH-tRNAPyl Deposited 2025-04-04	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric	Chain u 1–94（94 aa）	Not recorded	PAR PAROMOMYCIN × 1 MG MAGNESIUM ION × 320 SPD SPERMIDINE × 13 FME N-FORMYLMETHIONINE × 1 SPM SPERMINE × 1 K POTASSIUM ION × 10 ZN ZINC ION × 2 A1B70 (2R)-6-[(tert-butoxycarbonyl)amino]-2-(hydroxymethyl)hexanoic acid × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.14 Å
9PIH E. coli 70S ribosome bound to Doxycycline Deposited 2025-07-10	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 54-meric	Chain u 1–94（94 aa）	Not recorded	ZN ZINC ION × 1 MG MAGNESIUM ION × 276 K POTASSIUM ION × 92 DXT (4S,4AR,5S,5AR,6R,12AS)-4-(DIMETHYLAMINO)-3,5,10,12,12A-PENTAHYDROXY-6-METHYL-1,11-DIOXO-1,4,4A,5,5A,6,11,12A-OCTAHYDROTETRACENE-2-CARBOXAMIDE × 6	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.40 Å
9PII E. coli 70S ribosome bound to Sarecycline Deposited 2025-07-10	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 54-meric	Chain u 1–94（94 aa）	Not recorded	ZN ZINC ION × 1 MG MAGNESIUM ION × 281 K POTASSIUM ION × 100 V7A Sarecycline × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.40 Å
9PIJ E. coli 70S ribosome bound to Minocycline Deposited 2025-07-10	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 54-meric	Chain u 1–94（94 aa）	Not recorded	ZN ZINC ION × 1 MG MAGNESIUM ION × 340 K POTASSIUM ION × 148 MIY (4S,4AS,5AR,12AS)-4,7-BIS(DIMETHYLAMINO)-3,10,12,12A-TETRAHYDROXY-1,11-DIOXO-1,4,4A,5,5A,6,11,12A-OCTAHYDROTETRACENE-2- CARBOXAMIDE × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.20 Å
9Q87 Principles of ion binding to RNA inferred from the analysis of a 1.55 Angstrom resolution bacterial ribosome structure - Part I: Mg2+ Deposited 2025-02-23	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric	Chain u 1–94（94 aa）	Not recorded	K POTASSIUM ION × 235 MG MAGNESIUM ION × 415 CU1 COPPER (I) ION × 5 ZN ZINC ION × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 1.55 Å
9QQQ Cryo-EM structure of SKM-70S ribosomal stalled complex in the major state (vacant A-site, canon) Deposited 2025-04-01	Different oligomeric state Different ligand/ion Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric	Chain u 1–94（94 aa）	Not recorded	ZN ZINC ION × 2 A1JA7 [(2~{S},3~{S},4~{R},5~{R},6~{S})-4-[(2~{S},3~{R},4~{S},5~{R},6~{S})-5-acetamido-6-(hydroxymethyl)-4-[(2~{R},3~{R},4~{S},5~{S},6~{R})-6-(hydroxymethyl)-4-[(2~{S},3~{R},4~{R},5~{R},6~{R})-6-(hydroxymethyl)-5-methoxy-3,4-bis(oxidanyl)oxan-2-yl]oxy-3,5-bis(oxidanyl)oxan-2-yl]oxy-3-oxidanyl-oxan-2-yl]oxy-6-carbamimidamido-2-(hydroxymethyl)-5-oxidanyl-oxan-3-yl] carbamate × 1 MG MAGNESIUM ION × 312	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE-PROPANE	Resolution 2.26 Å
9QSJ Cryo-EM structure of SKM-70S ribosomal stalled complex in the A-tRNA positioned (Body open) state. Deposited 2025-04-05	Different oligomeric state Different ligand/ion Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric	Chain u 1–94（94 aa）	Not recorded	ZN ZINC ION × 2 A1JAI [(2~{S},3~{S},4~{R},5~{R},6~{S})-4-[(2~{S},3~{R},4~{S},5~{R},6~{S})-5-acetamido-6-(hydroxymethyl)-4-[(2~{R},3~{R},4~{S},5~{S},6~{R})-6-(hydroxymethyl)-4-[(2~{S},3~{R},4~{R},5~{R},6~{R})-6-(hydroxymethyl)-5-methoxy-3,4-bis(oxidanyl)oxan-2-yl]oxy-3,5-bis(oxidanyl)oxan-2-yl]oxy-3-oxidanyl-oxan-2-yl]oxy-2-(hydroxymethyl)-6-[[~{N}-[4-[(~{N}-methylcarbamimidoyl)amino]butyl]carbamimidoyl]amino]-5-oxidanyl-oxan-3-yl] carbamate × 1 MG MAGNESIUM ION × 311	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE-PROPANE	Resolution 2.62 Å
9R3A Structure of Stalled Beta-Galactosidase 70S Ribosome Nascent Chain Deposited 2025-05-03	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric	Chain u 1–94（94 aa）	Not recorded	ZN ZINC ION × 2 K POTASSIUM ION × 131 MG MAGNESIUM ION × 287 PRO PROLINE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.2 cryo-EM vitrification conditions Cryogen ETHANE-PROPANE	Resolution 2.86 Å
9RBF Structure of a stalled E. coli 70S RNC-NuoK-86 in complex with the membrane protein insertase SecYEG-YidC Deposited 2025-05-22	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 54 PDB declaration: 61-meric	Chain u 1–94（94 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY mmCIF provides none of the parsed conditions	Resolution 2.44 Å
9RFW Manikomycin bound to the Escherichia coli 50S ribosomal subunit Deposited 2025-06-05	Different oligomeric state Different ligand/ion Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 31 PDB declaration: 35-meric	Chain u 1–94（94 aa）	Not recorded	ZN ZINC ION × 2 MG MAGNESIUM ION × 213 K POTASSIUM ION × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE-PROPANE	Resolution 2.40 Å
9RJA Manikomycin bound to the Escherichia coli 70S ribosome Deposited 2025-06-12	Different oligomeric state Different ligand/ion Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 57-meric	Chain u 1–94（94 aa）	Not recorded	ZN ZINC ION × 2 MG MAGNESIUM ION × 309 K POTASSIUM ION × 2 PAR PAROMOMYCIN × 1 SPD SPERMIDINE × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE-PROPANE	Resolution 2.45 Å
9RTU Structure of the 70S-EF-G(P610L)-GDP-Pi ribosome complex with tRNAs in hybrid state 1 (H1-EF-G(P610L)-GDP-Pi) Deposited 2025-07-03	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 53 PDB declaration: 59-meric	Chain V 1–94（94 aa）	Not recorded	MG MAGNESIUM ION × 369 ZN ZINC ION × 2 NA SODIUM ION × 2 AM2 APRAMYCIN × 3 PO4 PHOSPHATE ION × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5；50 mM HEPES, 70 mM NH4Cl, 30 mM KCl, 3.5 mM MgCl2, 0.6 mM spermine, 0.4 mM spermidine cryo-EM vitrification conditions Cryogen ETHANE；Manual blotting & plunge-freezing	Resolution 3.00 Å
9RTV Structure of the 70S-EF-G(P610L)-GDP-Pi ribosome complex with tRNAs in hybrid state 2 (H2-EF-G(P610L)-GDP-Pi) Deposited 2025-07-03	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 53 PDB declaration: 59-meric	Chain V 1–94（94 aa）	Not recorded	MG MAGNESIUM ION × 369 ZN ZINC ION × 2 NA SODIUM ION × 2 AM2 APRAMYCIN × 3 GDP GUANOSINE-5'-DIPHOSPHATE × 1 PO4 PHOSPHATE ION × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5；50 mM HEPES, 70 mM NH4Cl, 30 mM KCl, 3.5 mM MgCl2, 0.6 mM spermine, 0.4 mM spermidine cryo-EM vitrification conditions Cryogen ETHANE；Manual blotting & plunge-freezing	Resolution 3.60 Å
9SLG Doxycycline Bound E. coli Ribosome with Rearranged Peptidyl Transferase Centre Deposited 2025-09-03	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 54-meric	Chain u 1–94（94 aa）	Not recorded	ZN ZINC ION × 2 MG MAGNESIUM ION × 447 K POTASSIUM ION × 77 DXT (4S,4AR,5S,5AR,6R,12AS)-4-(DIMETHYLAMINO)-3,5,10,12,12A-PENTAHYDROXY-6-METHYL-1,11-DIOXO-1,4,4A,5,5A,6,11,12A-OCTAHYDROTETRACENE-2-CARBOXAMIDE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5；BS100 ribosomal resuspension buffer cryo-EM vitrification conditions Cryogen ETHANE；Blot time 4 s, blot force 1, blot total 1	Resolution 2.16 Å
9SRO Cryo-EM structure of SKM-70S ribosomal stalled complex in the rotated state with hybrid tRNAs Deposited 2025-09-24	Different oligomeric state Different ligand/ion Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 54-meric	Chain u 1–94（94 aa）	Not recorded	ZN ZINC ION × 2 MG MAGNESIUM ION × 309 A1JAI [(2~{S},3~{S},4~{R},5~{R},6~{S})-4-[(2~{S},3~{R},4~{S},5~{R},6~{S})-5-acetamido-6-(hydroxymethyl)-4-[(2~{R},3~{R},4~{S},5~{S},6~{R})-6-(hydroxymethyl)-4-[(2~{S},3~{R},4~{R},5~{R},6~{R})-6-(hydroxymethyl)-5-methoxy-3,4-bis(oxidanyl)oxan-2-yl]oxy-3,5-bis(oxidanyl)oxan-2-yl]oxy-3-oxidanyl-oxan-2-yl]oxy-2-(hydroxymethyl)-6-[[~{N}-[4-[(~{N}-methylcarbamimidoyl)amino]butyl]carbamimidoyl]amino]-5-oxidanyl-oxan-3-yl] carbamate × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE-PROPANE	Resolution 2.60 Å
9SS4 pre-50S ribosomal subunit in rimM-KO with RsfS (-H68) Deposited 2025-09-25	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 33 PDB declaration: 35-meric	Chain BY 1–94（94 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.70 Å
9SS5 50S ribosomal subunit in rimM-KO with RsfS (+H68) Deposited 2025-09-25	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 32 PDB declaration: 34-meric	Chain BY 1–94（94 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.70 Å
9SS6 70S ribosome from RimM-KO Deposited 2025-09-25	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 55-meric	Chain BY 1–94（94 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.60 Å
9SYH E. coli 70S ribosome from deltaRlmE strain Deposited 2025-10-12	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric	Chain u 1–94（94 aa）	Not recorded	K POTASSIUM ION × 148 MG MAGNESIUM ION × 351 SR0 N~1~-(3-azaniumylpropyl)butane-1,4-diaminium × 6 PUT 1,4-DIAMINOBUTANE × 9 ZN ZINC ION × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5；HEPES-polymix buffer (pH-7.5) cryo-EM vitrification conditions Cryogen ETHANE	Resolution 1.89 Å
9T0Y E. coli 70S ribosome from delta-9 strain Deposited 2025-10-20	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric	Chain u 1–94（94 aa）	Not recorded	K POTASSIUM ION × 143 MG MAGNESIUM ION × 337 SR0 N~1~-(3-azaniumylpropyl)butane-1,4-diaminium × 6 PUT 1,4-DIAMINOBUTANE × 9 ZN ZINC ION × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5；HEPES-polymix buffer (pH-7.5) cryo-EM vitrification conditions Cryogen ETHANE	Resolution 1.87 Å
9T6M E. coli 70S ribosome from delta-10 strain Deposited 2025-11-07	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric	Chain u 1–94（94 aa）	Not recorded	K POTASSIUM ION × 132 MG MAGNESIUM ION × 316 SR0 N~1~-(3-azaniumylpropyl)butane-1,4-diaminium × 5 PUT 1,4-DIAMINOBUTANE × 8 ZN ZINC ION × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5；HEPES-polymix buffer (pH-7.5) cryo-EM vitrification conditions Cryogen ETHANE	Resolution 1.87 Å
9TEW DalDro bound to the initiating Escherichia coli 70S ribosome Deposited 2025-11-26	Different oligomeric state Different ligand/ion Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric	Chain u 1–94（94 aa）	Not recorded	ZN ZINC ION × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE-PROPANE	Resolution 2.90 Å
9TEX Dal2 bound to the Escherichia coli 70S ribosome Deposited 2025-11-26	Different oligomeric state Different ligand/ion Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric	Chain u 1–94（94 aa）	Not recorded	ZN ZINC ION × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE-PROPANE	Resolution 2.54 Å
9TEZ DalDroS bound to the Escherichia coli 50S ribosomal subunit Deposited 2025-11-26	Different oligomeric state Different ligand/ion Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 31 PDB declaration: 34-meric	Chain u 1–94（94 aa）	Not recorded	ZN ZINC ION × 2 SPM SPERMINE × 1 MG MAGNESIUM ION × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE-PROPANE	Resolution 2.88 Å
9TMI E. coli 70S ribosome with A- and P-site tRNA Deposited 2025-12-13	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric	Chain u 1–94（94 aa）	Not recorded	ZN ZINC ION × 2 MG MAGNESIUM ION × 258 SCM SPECTINOMYCIN × 1 VAL VALINE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.08 Å
9TNL E. coli 70S ribosome with A/P- and P/E-site tRNA Deposited 2025-12-16	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric	Chain u 1–94（94 aa）	Not recorded	ZN ZINC ION × 2 SCM SPECTINOMYCIN × 1 MG MAGNESIUM ION × 266 K POTASSIUM ION × 52 VAL VALINE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.11 Å
9TQA E. coli 70S ribosome, trapped conformational ground state of SSU-h44 apical loop, with A- and P-site tRNA Deposited 2025-12-19	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric	Chain u 1–94（94 aa）	Not recorded	ZN ZINC ION × 2 SCM SPECTINOMYCIN × 1 MG MAGNESIUM ION × 238 VAL VALINE × 1 K POTASSIUM ION × 6	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.06 Å
9WNQ Structure of E.coli ribosome in complex with an engineered arrest peptide Deposited 2025-09-05	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric	Chain w 1–94（94 aa）	Not recorded	ZN ZINC ION × 1 PRO PROLINE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6；20 mM HEPES (pH 7.6), 140 mM NH4Cl, 25 mM MgCl2, 2 mM DTT cryo-EM vitrification conditions Cryogen NITROGEN	Resolution 2.59 Å
9WNR Structure of E.coli ribosome in complex with an engineered arrest peptide and trigger factor Deposited 2025-09-05	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 57-meric	Chain w 1–94（94 aa）	Not recorded	ZN ZINC ION × 1 PRO PROLINE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6；20 mM HEPES (pH 7.6), 140 mM NH4Cl, 25 mM MgCl2, 2 mM DTT cryo-EM vitrification conditions Cryogen NITROGEN	Resolution 2.76 Å
9XFK In situ structure of bacterial 50S ribosomes Deposited 2025-10-29	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 29 PDB declaration: 31-meric	Chain d 1–94（94 aa）	Not recorded	MG MAGNESIUM ION × 166 NA SODIUM ION × 1 ZN ZINC ION × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.98 Å
9XFL In vitro structure of bacterial 50S ribosomes Deposited 2025-10-29	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 29 PDB declaration: 31-meric	Chain d 1–94（94 aa）	Not recorded	MG MAGNESIUM ION × 176 NA SODIUM ION × 1 ZN ZINC ION × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.7 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.16 Å
9Y79 Escherichia coli transcription-translation loosely coupled complex (TTC-LC^walked) containing mRNA with a 39 nt long spacer, NusG, NusA, and fMet-tRNAs in E-site and P-site Deposited 2025-09-09	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Other combination Heteromer；Protein × 60 PDB declaration: 68-meric	Chain 4 1–94（94 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY mmCIF provides none of the parsed conditions	Resolution 3.20 Å
9ZTL Single Particle Cryo EM Analysis of a Ribosome Nascent Globin Complex Deposited 2025-12-23	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric	Chain u 1–94（94 aa）	Not recorded	ZN ZINC ION × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.91 Å

478 other PDB entries and 523 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence

UniProt name	RL25_ECOLI
Isoform	—
PDB entities	27
Chains and sequence ranges	Author chain u； PDBConstruct 1–94； UniProt 1–94

50S ribosomal protein L27

OrganismNot specified

UniProt C3SSG7

State in the Current Structure

Assembly	Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Associated Components	Method and Experimental Conditions	Structure Quality
1	Protein–RNA Heteromer Protein × 30 RNA 2 PDB declaration: 32-meric（32） Consistent with all polymer counts	Chain v； UniProt 1–85	Not recorded	50S ribosomal protein L33 × 1 （A0A3S5YPG8） 50S ribosomal protein L34 × 1 （A0A8X6EVB8） 50S ribosomal protein L35 × 1 （A0A8S0FT45） 50S ribosomal protein L36 × 1 （A0A2X1PTA3） 50S ribosomal protein L31 × 1 （C3SIV2） Drosocin1 × 1 （B4MNS1） 23S ribosomal RNA × 1 5S ribosomal RNA × 1 50S ribosomal protein L2 × 1 （A0A7L6YWH1） 50S ribosomal protein L3 × 1 （C3SQU2） 50S ribosomal protein L4 × 1 （D8A1N0） 50S ribosomal protein L5 × 1 （D6IEK6） 50S ribosomal protein L6 × 1 （C3SR17） 50S ribosomal protein L9 × 1 （C3SFP2） 50S ribosomal protein L13 × 1 （A0A656YGQ7） 50S ribosomal protein L14 × 1 （C3SQZ2） 50S ribosomal protein L15 × 1 （P02413） 50S ribosomal protein L16 × 1 （A0A0H3EMH6） 50S ribosomal protein L17 × 1 （A0A1X3JBW6） 50S ribosomal protein L18 × 1 （A0A514EKV0） 50S ribosomal protein L19 × 1 （A0A370V6W8） 50S ribosomal protein L20 × 1 （A0A378BCJ4） 50S ribosomal protein L21 × 1 （A0A0E2L2H1） 50S ribosomal protein L22 × 1 （A0A0F6B7N1） 50S ribosomal protein L23 × 1 （D7Y3D8） 50S ribosomal protein L24 × 1 （S1E9P3） 50S ribosomal protein L25 × 1 （P68919） 50S ribosomal protein L28 × 1 （C3SME2） 50S ribosomal protein L29 × 1 （P0A7M6） 50S ribosomal protein L30 × 1 （C3SR32） 50S ribosomal protein L32 × 1 （A0A657HW13） ZN ZINC ION × 2 A2G 2-acetamido-2-deoxy-alpha-D-galactopyranose × 1 MG MAGNESIUM ION × 216 SPM SPERMINE × 1	ELECTRON MICROSCOPY cryo-EM buffer：pH 7.4 cryo-EM vitrification conditions：Cryogen ETHANE-PROPANE	Resolution 2.10 Å

Other States of the Same Protein in the Database

Other PDB	Difference from Current Entry 8ANA	Assembly / Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Non-polymers	Method and Experimental Conditions	Structure Quality
4V85 Crystal Structure of Release Factor RF3 Trapped in the GTP State on a Rotated Conformation of the Ribosome. Deposited 2011-06-13	Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 55 PDB declaration: 59-meric	Chain B0 1–85（85 aa）	Not recorded	MG MAGNESIUM ION × 493 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；295 K；Tris Ac PH.7.0, 25-35 mM KCL, 6.1% PEG 20000, 1% glycerol, 50mM sucrose, VAPOR DIFFUSION, SITTING DROP, temperature 295K	Resolution 3.20 Å R-free 0.250
8AKN Cryo-EM structure of the proline-rich antimicrobial peptide drosocin bound to the terminating ribosome Deposited 2022-07-30	Different oligomeric state Different ligand/ion Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 57-meric	Chain v 1–85（85 aa）	Not recorded	ZN ZINC ION × 2 A2G 2-acetamido-2-deoxy-alpha-D-galactopyranose × 1 MG MAGNESIUM ION × 305 SPM SPERMINE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE-PROPANE	Resolution 2.30 Å
8AM9 Cryo-EM structure of the proline-rich antimicrobial peptide drosocin bound to the elongating ribosome Deposited 2022-08-03	Different oligomeric state Different ligand/ion Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric	Chain v 1–85（85 aa）	Not recorded	ZN ZINC ION × 2 MG MAGNESIUM ION × 293 SPM SPERMINE × 1 A2G 2-acetamido-2-deoxy-alpha-D-galactopyranose × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE-PROPANE	Resolution 2.80 Å
8G2U Time-resolved cryo-EM study of the 70S recycling by the HflX:control-apo-70S at 900ms Deposited 2023-02-06	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 51-meric	Chain W 7–85（79 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.00 Å
8G31 Time-resolved cryo-EM study of the 70S recycling by the HflX:2nd Intermediate Deposited 2023-02-06	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 52-meric	Chain W 7–85（79 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.20 Å
8G34 Time-resolved cryo-EM study of the 70S recycling by the HflX:1st intermediate Deposited 2023-02-06	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 52-meric	Chain W 7–85（79 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.20 Å
8G38 Time-resolved cryo-EM study of the 70S recycling by the HflX:3rd Intermediate Deposited 2023-02-07	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 52-meric	Chain W 7–85（79 aa）	Not recorded	GTP GUANOSINE-5'-TRIPHOSPHATE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.20 Å
8T5D Cryo-EM studies of the interplay between uS2 ribosomal protein and leaderless mRNA during bacterial translation initiation Deposited 2023-06-13	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 53-meric	Chain W 7–85（79 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.20 Å
8T5H Cryo-EM studies of the interplay between uS2 ribosomal protein and leaderless mRNA during bacterial translation initiation Deposited 2023-06-13	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 46 PDB declaration: 51-meric	Chain W 7–85（79 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.30 Å
8URM E. coli 70S ribosome with unmodified tRNAPro(GGG) bound to slippery P-site CCC-C codon and tRNAVal(UAC) in the A site Deposited 2023-10-26	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric	Chain W 2–85（84 aa）	Not recorded	MG MAGNESIUM ION × 395	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.00 Å
8UTJ E. coli 70S ribosome with unmodified lys-tRNAPro(GGG) bound to slippery P-site CCC-C codon in the 0 frame Deposited 2023-10-31	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric	Chain W 2–85（84 aa）	Not recorded	MG MAGNESIUM ION × 530 LYS LYSINE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.50 Å
8UX8 E. coli 70S ribosome with unmodified Lys-tRNAPro(GGG) bound to slippery P-site CCC-C codon in the +1 mRNA reading frame Deposited 2023-11-09	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric	Chain W 2–85（84 aa）	Not recorded	MG MAGNESIUM ION × 395 LYS LYSINE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.60 Å
8UXB E. coli 70S ribosome with unmodified P/E-tRNAPro(GGG) bound to slippery P-site CCC-C codon Deposited 2023-11-09	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 53-meric	Chain W 2–85（84 aa）	Not recorded	MG MAGNESIUM ION × 287	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.90 Å
8UZ3 E. coli 70S ribosome with unmodified e*/E-tRNAPro(GGG) bound to slippery P-site CCC-C codon Deposited 2023-11-14	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 53-meric	Chain W 2–85（84 aa）	Not recorded	MG MAGNESIUM ION × 415	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.20 Å
8UZG E. coli 70S ribosome with unmodified e*/E-tRNAPro(GGG) bound to slippery P-site CCC-C codon Deposited 2023-11-15	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 53-meric	Chain W 2–85（84 aa）	Not recorded	MG MAGNESIUM ION × 398	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.70 Å
8V03 E. coli 70S ribosome with unmodified P/E-tRNAPro(GGG) bound to slippery P-site CCC-C codon Deposited 2023-11-16	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 53-meric	Chain W 2–85（84 aa）	Not recorded	MG MAGNESIUM ION × 281	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 4.00 Å
8VV4 E. coli 70S ribosome with unmodified Lys-tRNAPro(GGG) in the P/P conformation on a slippery CCC-C codon Deposited 2024-01-30	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 54-meric	Chain W 2–85（84 aa）	Not recorded	MG MAGNESIUM ION × 424 LYS LYSINE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.40 Å
8VWQ E. coli 70S ribosome with unmodified Lys-tRNAPro(GGG) in the P/P conformation on a slippery CCC-C codon and Elongation Factor P bound (uL1 in the closed conformation) Deposited 2024-02-02	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 56-meric	Chain W 2–85（84 aa）	Not recorded	MG MAGNESIUM ION × 586 LYS LYSINE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.10 Å
8W2N E. coli 70S ribosome with unmodified Lys-tRNAPro(GGG) in the P/P conformation on a slippery CCC-C codon and Elongation Factor P bound (uL1 in the open conformation) Deposited 2024-02-20	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric	Chain W 2–85（84 aa）	Not recorded	MG MAGNESIUM ION × 481	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.20 Å
9AX8 70S initiation complex (tRNA-fMet M1, initiation factor 2 + CUG start codon) Deposited 2024-03-06	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 53-meric	Chain 0 7–85（79 aa）	Not recorded	MG MAGNESIUM ION × 287 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.60 Å

20 other PDB entries and 20 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence

UniProt name	C3SSG7_ECOLX
Isoform	—
PDB entities	28
Chains and sequence ranges	Author chain v； PDBConstruct 1–85； UniProt 1–85

50S ribosomal protein L28

OrganismNot specified

UniProt C3SME2

State in the Current Structure

Assembly	Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Associated Components	Method and Experimental Conditions	Structure Quality
1	Protein–RNA Heteromer Protein × 30 RNA 2 PDB declaration: 32-meric（32） Consistent with all polymer counts	Chain w； UniProt 1–78	Not recorded	50S ribosomal protein L33 × 1 （A0A3S5YPG8） 50S ribosomal protein L34 × 1 （A0A8X6EVB8） 50S ribosomal protein L35 × 1 （A0A8S0FT45） 50S ribosomal protein L36 × 1 （A0A2X1PTA3） 50S ribosomal protein L31 × 1 （C3SIV2） Drosocin1 × 1 （B4MNS1） 23S ribosomal RNA × 1 5S ribosomal RNA × 1 50S ribosomal protein L2 × 1 （A0A7L6YWH1） 50S ribosomal protein L3 × 1 （C3SQU2） 50S ribosomal protein L4 × 1 （D8A1N0） 50S ribosomal protein L5 × 1 （D6IEK6） 50S ribosomal protein L6 × 1 （C3SR17） 50S ribosomal protein L9 × 1 （C3SFP2） 50S ribosomal protein L13 × 1 （A0A656YGQ7） 50S ribosomal protein L14 × 1 （C3SQZ2） 50S ribosomal protein L15 × 1 （P02413） 50S ribosomal protein L16 × 1 （A0A0H3EMH6） 50S ribosomal protein L17 × 1 （A0A1X3JBW6） 50S ribosomal protein L18 × 1 （A0A514EKV0） 50S ribosomal protein L19 × 1 （A0A370V6W8） 50S ribosomal protein L20 × 1 （A0A378BCJ4） 50S ribosomal protein L21 × 1 （A0A0E2L2H1） 50S ribosomal protein L22 × 1 （A0A0F6B7N1） 50S ribosomal protein L23 × 1 （D7Y3D8） 50S ribosomal protein L24 × 1 （S1E9P3） 50S ribosomal protein L25 × 1 （P68919） 50S ribosomal protein L27 × 1 （C3SSG7） 50S ribosomal protein L29 × 1 （P0A7M6） 50S ribosomal protein L30 × 1 （C3SR32） 50S ribosomal protein L32 × 1 （A0A657HW13） ZN ZINC ION × 2 A2G 2-acetamido-2-deoxy-alpha-D-galactopyranose × 1 MG MAGNESIUM ION × 216 SPM SPERMINE × 1	ELECTRON MICROSCOPY cryo-EM buffer：pH 7.4 cryo-EM vitrification conditions：Cryogen ETHANE-PROPANE	Resolution 2.10 Å

Other States of the Same Protein in the Database

Other PDB	Difference from Current Entry 8ANA	Assembly / Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Non-polymers	Method and Experimental Conditions	Structure Quality
4UY8 Molecular basis for the ribosome functioning as a L-tryptophan sensor - Cryo-EM structure of a TnaC stalled E.coli ribosome Deposited 2014-08-29	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 32 PDB declaration: 35-meric	Chain X 2–78（77 aa）	Not recorded	MG MAGNESIUM ION × 143 ZN ZINC ION × 1 TRP TRYPTOPHAN × 2	ELECTRON MICROSCOPY cryo-EM vitrification conditions Cryogen ETHANE；VITRIFICATION 1 --CRYOGEN- ETHANE,INSTRUMENT- FEI VITROBOT MARK IV	Resolution 3.80 Å
4V85 Crystal Structure of Release Factor RF3 Trapped in the GTP State on a Rotated Conformation of the Ribosome. Deposited 2011-06-13	Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 55 PDB declaration: 59-meric	Chain B1 1–78（78 aa）	Not recorded	MG MAGNESIUM ION × 493 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；295 K；Tris Ac PH.7.0, 25-35 mM KCL, 6.1% PEG 20000, 1% glycerol, 50mM sucrose, VAPOR DIFFUSION, SITTING DROP, temperature 295K	Resolution 3.20 Å R-free 0.250
8AKN Cryo-EM structure of the proline-rich antimicrobial peptide drosocin bound to the terminating ribosome Deposited 2022-07-30	Different oligomeric state Different ligand/ion Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 57-meric	Chain w 1–78（78 aa）	Not recorded	ZN ZINC ION × 2 A2G 2-acetamido-2-deoxy-alpha-D-galactopyranose × 1 MG MAGNESIUM ION × 305 SPM SPERMINE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE-PROPANE	Resolution 2.30 Å
8AM9 Cryo-EM structure of the proline-rich antimicrobial peptide drosocin bound to the elongating ribosome Deposited 2022-08-03	Different oligomeric state Different ligand/ion Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric	Chain w 1–78（78 aa）	Not recorded	ZN ZINC ION × 2 MG MAGNESIUM ION × 293 SPM SPERMINE × 1 A2G 2-acetamido-2-deoxy-alpha-D-galactopyranose × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE-PROPANE	Resolution 2.80 Å

4 other PDB entries and 4 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence

UniProt name	C3SME2_ECOLX
Isoform	—
PDB entities	29
Chains and sequence ranges	Author chain w； PDBConstruct 1–78； UniProt 1–78

50S ribosomal protein L29

OrganismNot specified

UniProt P0A7M6

State in the Current Structure

Assembly	Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Associated Components	Method and Experimental Conditions	Structure Quality
1	Protein–RNA Heteromer Protein × 30 RNA 2 PDB declaration: 32-meric（32） Consistent with all polymer counts	Chain x； UniProt 1–63	Not recorded	50S ribosomal protein L33 × 1 （A0A3S5YPG8） 50S ribosomal protein L34 × 1 （A0A8X6EVB8） 50S ribosomal protein L35 × 1 （A0A8S0FT45） 50S ribosomal protein L36 × 1 （A0A2X1PTA3） 50S ribosomal protein L31 × 1 （C3SIV2） Drosocin1 × 1 （B4MNS1） 23S ribosomal RNA × 1 5S ribosomal RNA × 1 50S ribosomal protein L2 × 1 （A0A7L6YWH1） 50S ribosomal protein L3 × 1 （C3SQU2） 50S ribosomal protein L4 × 1 （D8A1N0） 50S ribosomal protein L5 × 1 （D6IEK6） 50S ribosomal protein L6 × 1 （C3SR17） 50S ribosomal protein L9 × 1 （C3SFP2） 50S ribosomal protein L13 × 1 （A0A656YGQ7） 50S ribosomal protein L14 × 1 （C3SQZ2） 50S ribosomal protein L15 × 1 （P02413） 50S ribosomal protein L16 × 1 （A0A0H3EMH6） 50S ribosomal protein L17 × 1 （A0A1X3JBW6） 50S ribosomal protein L18 × 1 （A0A514EKV0） 50S ribosomal protein L19 × 1 （A0A370V6W8） 50S ribosomal protein L20 × 1 （A0A378BCJ4） 50S ribosomal protein L21 × 1 （A0A0E2L2H1） 50S ribosomal protein L22 × 1 （A0A0F6B7N1） 50S ribosomal protein L23 × 1 （D7Y3D8） 50S ribosomal protein L24 × 1 （S1E9P3） 50S ribosomal protein L25 × 1 （P68919） 50S ribosomal protein L27 × 1 （C3SSG7） 50S ribosomal protein L28 × 1 （C3SME2） 50S ribosomal protein L30 × 1 （C3SR32） 50S ribosomal protein L32 × 1 （A0A657HW13） ZN ZINC ION × 2 A2G 2-acetamido-2-deoxy-alpha-D-galactopyranose × 1 MG MAGNESIUM ION × 216 SPM SPERMINE × 1	ELECTRON MICROSCOPY cryo-EM buffer：pH 7.4 cryo-EM vitrification conditions：Cryogen ETHANE-PROPANE	Resolution 2.10 Å

Other States of the Same Protein in the Database

Other PDB	Difference from Current Entry 8ANA	Assembly / Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Non-polymers	Method and Experimental Conditions	Structure Quality
28UH E. coli 70S ribosome, trapped conformational ground state of SSU-h44 apical loop, with A/P- and P/E-site tRNA Deposited 2026-02-20	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric	Chain x 1–63（63 aa）	Not recorded	ZN ZINC ION × 2 MG MAGNESIUM ION × 274 K POTASSIUM ION × 65 SCM SPECTINOMYCIN × 1 VAL VALINE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.06 Å
28UI E. coli 70S ribosome, trapped conformational excited state of SSU-h44 apical loop, with A- and P-site tRNA Deposited 2026-02-20	Different oligomeric state Different ligand/ion Different experimental conditions	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric	Chain x 1–63（63 aa）	Not recorded	ZN ZINC ION × 2 SCM SPECTINOMYCIN × 1 MG MAGNESIUM ION × 270 VAL VALINE × 1 K POTASSIUM ION × 11	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.10 Å
28UJ E. coli 70S ribosome, trapped conformational excited state of SSU-h44 apical loop, with A/P- and P/E-site tRNA Deposited 2026-02-20	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric	Chain x 1–63（63 aa）	Not recorded	ZN ZINC ION × 2 MG MAGNESIUM ION × 251 SCM SPECTINOMYCIN × 1 K POTASSIUM ION × 47 VAL VALINE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.16 Å
28UK E. coli 70S ribosome, conformational ground state mutation in SSU-h41, with A- and P-site tRNA Deposited 2026-02-20	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric	Chain x 1–63（63 aa）	Not recorded	ZN ZINC ION × 2 MG MAGNESIUM ION × 227 K POTASSIUM ION × 74 SCM SPECTINOMYCIN × 1 VAL VALINE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.24 Å
28UL E. coli 70S ribosome, conformational ground state mutation in SSU-h41, with A/P- and P/E-site tRNA Deposited 2026-02-20	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric	Chain x 1–63（63 aa）	Not recorded	ZN ZINC ION × 2 MG MAGNESIUM ION × 212 K POTASSIUM ION × 77 SCM SPECTINOMYCIN × 1 VAL VALINE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.50 Å
28UM E. coli 70S ribosome, conformational excited state mutation in SSU-h41, with A- and P-site tRNA Deposited 2026-02-20	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric	Chain x 1–63（63 aa）	Not recorded	ZN ZINC ION × 2 MG MAGNESIUM ION × 238 K POTASSIUM ION × 109 SCM SPECTINOMYCIN × 1 VAL VALINE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.15 Å
28UN E. coli 70S ribosome, conformational excited state mutation in SSU-h41, with A/P- and P/E-site tRNA Deposited 2026-02-20	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric	Chain x 1–63（63 aa）	Not recorded	ZN ZINC ION × 2 MG MAGNESIUM ION × 183 K POTASSIUM ION × 65 SCM SPECTINOMYCIN × 1 VAL VALINE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.48 Å
2J28 MODEL OF E. COLI SRP BOUND TO 70S RNCS Deposited 2006-08-16	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 31 PDB declaration: 34-meric	Chain X 1–63（63 aa）	Not recorded	MG MAGNESIUM ION × 111	ELECTRON MICROSCOPY cryo-EM vitrification conditions Cryogen ETHANE	Resolution 9.50 Å
2RDO 50S subunit with EF-G(GDPNP) and RRF bound Deposited 2007-09-24	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 32 PDB declaration: 34-meric	Chain X 1–63（63 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer PolyMix；pH 7.5；PolyMix cryo-EM vitrification conditions Cryogen ETHANE；Rapid-freezing in liquid ethane by Vitrobot	Resolution 9.10 Å
2VRH Structure of the E. coli trigger factor bound to a translating ribosome Deposited 2008-04-07	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric	Chain D 1–63（63 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer 50 MM HEPES-KOH PH 7.5, 100 MM KCL, 25 MM MGCL2, 0.5 MG/ML CHLORAMPHENICOL；pH 7.5；50 MM HEPES-KOH PH 7.5, 100 MM KCL, 25 MM MGCL2, 0.5 MG/ML CHLORAMPHENICOL cryo-EM vitrification conditions Cryogen ETHANE；LIQUID ETHANE	Resolution 19.00 Å
30QZ E. coli 50S ribosome with peptidyl-tRNA and Hsp15 Deposited 2026-05-10	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 30 PDB declaration: 33-meric	Chain x 1–63（63 aa）	Not recorded	MG MAGNESIUM ION × 204 ZN ZINC ION × 1 ASP ASPARTIC ACID × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5；HEPES-polymix buffer (pH-7.5) cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.23 Å
30RA E. coli 70S ribosome with peptidyl-tRNA, mRNA and Hsp15 Deposited 2026-05-10	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric	Chain x 1–63（63 aa）	Not recorded	ZN ZINC ION × 2 MG MAGNESIUM ION × 281 ASP ASPARTIC ACID × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5；HEPES-polymix buffer (pH-7.5) cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.78 Å
30RB E. coli 70S ribosome with peptidyl-tRNA and Hsp15 Deposited 2026-05-10	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric	Chain x 1–63（63 aa）	Not recorded	ZN ZINC ION × 1 MG MAGNESIUM ION × 210 K POTASSIUM ION × 2 ASP ASPARTIC ACID × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5；HEPES-polymix buffer (pH-7.5) cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.92 Å
3BBX The Hsp15 protein fitted into the low resolution Cryo-EM map of the 50S.nc-tRNA.Hsp15 complex Deposited 2007-11-11	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 28 PDB declaration: 30-meric	Chain X 1–63（63 aa）	Not recorded	MG MAGNESIUM ION × 110	ELECTRON MICROSCOPY cryo-EM vitrification conditions Cryogen ETHANE	Resolution 10.00 Å
3IY9 Leishmania Tarentolae Mitochondrial Large Ribosomal Subunit Model Deposited 2009-04-20	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 19 PDB declaration: eicosameric	Chain X 1–63（63 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer 50 mM Tris HCL, pH 7.5, 100mM KCL, 10mM MgCl2, 3mM DTT, 0.1mM EDTA, 0.05% dodecyl maltoside；pH 7.5；50 mM Tris HCL, pH 7.5, 100mM KCL, 10mM MgCl2, 3mM DTT, 0.1mM EDTA, 0.05% dodecyl maltoside cryo-EM vitrification conditions Cryogen ETHANE	Resolution 14.10 Å
3J45 Structure of a non-translocating SecY protein channel with the 70S ribosome Deposited 2013-06-18	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 6 PDB declaration: undecameric	Chain Y 1–63（63 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer 50 mM HEPES-KOH, 100 mM KOAc, 10 mM Mg(OAc)2, 0.05% DDM；pH 7.5；50 mM HEPES-KOH, 100 mM KOAc, 10 mM Mg(OAc)2, 0.05% DDM cryo-EM vitrification conditions 90 K；Cryogen ETHANE；Blot 1 second before plunging into liquid ethane (HOMEMADE PLUNGER).	Resolution 9.50 Å
3J46 Structure of the SecY protein translocation channel in action Deposited 2013-06-18	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 8 PDB declaration: tetradecameric	Chain Y 1–63（63 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer 50 mM Tris-acetate, 10 mM Mg(OAc)2, 80 mM KOAc, 0.06% DDM；pH 7.2；50 mM Tris-acetate, 10 mM Mg(OAc)2, 80 mM KOAc, 0.06% DDM cryo-EM vitrification conditions 77 K；Cryogen ETHANE；Blot 1-2 seconds before plunging into liquid ethane (FEI VITROBOT MARK III).	Resolution 10.10 Å
3J5L Structure of the E. coli 50S subunit with ErmBL nascent chain Deposited 2013-10-23	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 30 PDB declaration: 34-meric	Chain Y 1–63（63 aa）	Not recorded	UNL UNKNOWN LIGAND × 1 ERY ERYTHROMYCIN A × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE；Plunged into liquid ethane (FEI VITROBOT MARK IV)	Resolution 6.60 Å
3J7Z Structure of the E. coli 50S subunit with ErmCL nascent chain Deposited 2014-08-27	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 32 PDB declaration: 35-meric	Chain Y 1–63（63 aa）	Not recorded	ERY ERYTHROMYCIN A × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE；Plunged into liquid ethane (FEI VITROBOT MARK IV).	Resolution 3.90 Å
3J9Y Cryo-EM structure of tetracycline resistance protein TetM bound to a translating E.coli ribosome Deposited 2015-03-23	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 53 PDB declaration: 58-meric	Chain Y 1–63（63 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer 50 mM HEPES-KOH, 100 mM KOAc, 25 mM Mg(OAc)2, 6 mM b-mercaptoethanol；pH 7.4；50 mM HEPES-KOH, 100 mM KOAc, 25 mM Mg(OAc)2, 6 mM b-mercaptoethanol cryo-EM vitrification conditions Cryogen ETHANE；Plunged into liquid ethane (FEI VITROBOT MARK IV)	Resolution 3.90 Å
3J9Z Activation of GTP Hydrolysis in mRNA-tRNA Translocation by Elongation Factor G Deposited 2015-03-27	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 53 PDB declaration: 58-meric	Chain LW 1–63（63 aa）	Not recorded	GTP GUANOSINE-5'-TRIPHOSPHATE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions 93 K；Cryogen ETHANE；Plunged into liquid ethane (FEI VITROBOT MARK IV)	Resolution 3.60 Å
3JA1 Activation of GTP Hydrolysis in mRNA-tRNA Translocation by Elongation Factor G Deposited 2015-03-30	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 53 PDB declaration: 58-meric	Chain L0 1–63（63 aa）	Not recorded	GTP GUANOSINE-5'-TRIPHOSPHATE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions 93 K；Cryogen ETHANE；Plunged into liquid ethane (FEI VITROBOT MARK IV)	Resolution 3.60 Å
3JBU Mechanisms of Ribosome Stalling by SecM at Multiple Elongation Steps Deposited 2015-10-16	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 54-meric	Chain 1 1–63（63 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer 20mM HEPES, 50mM KOAc, 6mM Mg(OAc)2, 1mM DTT, 500 ug/ml chloramphenicol, 0.05% Nikkol, 0.5% pill/ml Complete EDTA-free Protease inhibitor cocktail, 0.1U/ml RNasin and 125mM sucrose；pH 7；20mM HEPES, 50mM KOAc, 6mM Mg(OAc)2, 1mM DTT, 500 ug/ml chloramphenicol, 0.05% Nikkol, 0.5% pill/ml Complete EDTA-free Protease inhibitor cocktail, 0.1U/ml RNasin and 125mM sucrose cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.64 Å
3JBV Mechanisms of Ribosome Stalling by SecM at Multiple Elongation Steps Deposited 2015-10-16	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric	Chain 1 1–63（63 aa）	Not recorded	CLM CHLORAMPHENICOL × 1	ELECTRON MICROSCOPY cryo-EM buffer 20mM HEPES, 50mM KOAc, 6mM Mg(OAc)2, 1mM DTT, 500 ug/ml chloramphenicol,0.05% Nikkol,0.5% pill/ml Complete EDTA-free Protease inhibitor cocktail,0.1 U/ml RNasin and 125mM sucrose；pH 7；20mM HEPES, 50mM KOAc, 6mM Mg(OAc)2, 1mM DTT, 500 ug/ml chloramphenicol,0.05% Nikkol,0.5% pill/ml Complete EDTA-free Protease inhibitor cocktail,0.1 U/ml RNasin and 125mM sucrose cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.32 Å
3JCD Structure of Escherichia coli EF4 in posttranslocational ribosomes (Post EF4) Deposited 2015-12-01	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 57-meric	Chain Y 1–63（63 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.70 Å
3JCE Structure of Escherichia coli EF4 in pretranslocational ribosomes (Pre EF4) Deposited 2015-12-01	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 58-meric	Chain Y 1–63（63 aa）	Not recorded	PHE PHENYLALANINE × 1 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1	ELECTRON MICROSCOPY cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.20 Å
3JCJ Structures of ribosome-bound initiation factor 2 reveal the mechanism of subunit association Deposited 2015-12-18	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 56-meric	Chain X 1–63（63 aa）	Not recorded	MG MAGNESIUM ION × 195 ZN ZINC ION × 1 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 FME N-FORMYLMETHIONINE × 1	ELECTRON MICROSCOPY cryo-EM buffer 20 mM HEPES-KOH, pH 7.5, 15 mM magnesium acetate, 150 mM potassium acetate, 4 mM 2-mercapthoethanol, 2 mM spermidine, 0.05 mM spermine；pH 7.5；20 mM HEPES-KOH, pH 7.5, 15 mM magnesium acetate, 150 mM potassium acetate, 4 mM 2-mercapthoethanol, 2 mM spermidine, 0.05 mM spermine cryo-EM vitrification conditions Blot for 2-4 seconds before plunging；Cryogen ETHANE；Blot for 2-4 seconds before plunging into liquid ethane (FEI VITROBOT MARK I).	Resolution 3.70 Å
3JCN Structures of ribosome-bound initiation factor 2 reveal the mechanism of subunit association: Initiation Complex I Deposited 2016-01-04	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric	Chain Y 1–63（63 aa）	Not recorded	GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 FME N-FORMYLMETHIONINE × 1	ELECTRON MICROSCOPY cryo-EM buffer 20 mM HEPES-KOH, pH 7.5, 15 mM magnesium acetate, 150 mM potassium acetate, 4 mM 2-mercapthoethanol, 2 mM spermidine, 0.05 mM spermine；pH 7.5；20 mM HEPES-KOH, pH 7.5, 15 mM magnesium acetate, 150 mM potassium acetate, 4 mM 2-mercapthoethanol, 2 mM spermidine, 0.05 mM spermine cryo-EM vitrification conditions Blot for 2-4 seconds before plunging；Cryogen ETHANE；Blot for 2-4 seconds before plunging into liquid ethane (FEI VITROBOT MARK I).	Resolution 4.60 Å
4CSU Cryo-EM structures of the 50S ribosome subunit bound with ObgE Deposited 2014-03-10	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 31 PDB declaration: 33-meric	Chain 1 1–63（63 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer 20MM TRIS-HCL, 100MM NH4CL,10MM MGCL2；pH 7.5；20MM TRIS-HCL, 100MM NH4CL,10MM MGCL2 cryo-EM vitrification conditions Cryogen ETHANE；LIQUID ETHANE	Resolution 5.50 Å
4U1U Crystal structure of the E. coli ribosome bound to quinupristin. Deposited 2014-07-16	Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 54-meric	Chain BY 1–63（63 aa）	Not recorded	MG MAGNESIUM ION × 271 ZN ZINC ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions EVAPORATION；pH 6.5；291 K；PEG8k, MPD	Resolution 2.95 Å R-free 0.282
4U1U Crystal structure of the E. coli ribosome bound to quinupristin. Deposited 2014-07-16	Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 53-meric	Chain DY 1–63（63 aa）	Not recorded	MG MAGNESIUM ION × 227 ZN ZINC ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions EVAPORATION；pH 6.5；291 K；PEG8k, MPD	Resolution 2.95 Å R-free 0.282
4U1V Crystal structure of the E. coli ribosome bound to linopristin. Deposited 2014-07-16	Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 54-meric	Chain BY 1–63（63 aa）	Not recorded	MG MAGNESIUM ION × 271 ZN ZINC ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions MICROBATCH；pH 6.5；291 K；PEG8k, MPD	Resolution 3.00 Å R-free 0.274
4U1V Crystal structure of the E. coli ribosome bound to linopristin. Deposited 2014-07-16	Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 53-meric	Chain DY 1–63（63 aa）	Not recorded	MG MAGNESIUM ION × 227 ZN ZINC ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions MICROBATCH；pH 6.5；291 K；PEG8k, MPD	Resolution 3.00 Å R-free 0.274
4U20 Crystal structure of the E. coli ribosome bound to flopristin. Deposited 2014-07-16	Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 53-meric	Chain BY 1–63（63 aa）	Not recorded	MG MAGNESIUM ION × 271 VIF Flopristin × 1 ZN ZINC ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions MICROBATCH；pH 6.5；291 K；PEG8k, MPD	Resolution 2.90 Å R-free 0.279
4U20 Crystal structure of the E. coli ribosome bound to flopristin. Deposited 2014-07-16	Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 52-meric	Chain DY 1–63（63 aa）	Not recorded	MG MAGNESIUM ION × 227 VIF Flopristin × 1 ZN ZINC ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions MICROBATCH；pH 6.5；291 K；PEG8k, MPD	Resolution 2.90 Å R-free 0.279
4U24 Crystal structure of the E. coli ribosome bound to dalfopristin. Deposited 2014-07-16	Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 53-meric	Chain BY 1–63（63 aa）	Not recorded	MG MAGNESIUM ION × 271 DOL 5-(2-DIETHYLAMINO-ETHANESULFONYL)-21-HYDROXY-10-ISOPROPYL-11,19-DIMETHYL-9,26-DIOXA-3,15,28-TRIAZA-TRICYCLO[23.2.1.00,255]OCTACOSA-1(27),12,17,19,25(28)-PENTAENE-2,8,14,23-TETRAONE × 1 ZN ZINC ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions MICROBATCH；pH 6.5；291 K；PEG8k, MPD	Resolution 2.90 Å R-free 0.264
4U24 Crystal structure of the E. coli ribosome bound to dalfopristin. Deposited 2014-07-16	Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 52-meric	Chain DY 1–63（63 aa）	Not recorded	MG MAGNESIUM ION × 226 DOL 5-(2-DIETHYLAMINO-ETHANESULFONYL)-21-HYDROXY-10-ISOPROPYL-11,19-DIMETHYL-9,26-DIOXA-3,15,28-TRIAZA-TRICYCLO[23.2.1.00,255]OCTACOSA-1(27),12,17,19,25(28)-PENTAENE-2,8,14,23-TETRAONE × 1 ZN ZINC ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions MICROBATCH；pH 6.5；291 K；PEG8k, MPD	Resolution 2.90 Å R-free 0.264
4U25 Crystal structure of the E. coli ribosome bound to virginiamycin M1. Deposited 2014-07-16	Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 53-meric	Chain BY 1–63（63 aa）	Not recorded	MG MAGNESIUM ION × 271 VIR VIRGINIAMYCIN M1 × 1 ZN ZINC ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions MICROBATCH；pH 6.5；291 K；PEG8k, MPD	Resolution 2.90 Å R-free 0.265
4U25 Crystal structure of the E. coli ribosome bound to virginiamycin M1. Deposited 2014-07-16	Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 52-meric	Chain DY 1–63（63 aa）	Not recorded	MG MAGNESIUM ION × 227 VIR VIRGINIAMYCIN M1 × 1 ZN ZINC ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions MICROBATCH；pH 6.5；291 K；PEG8k, MPD	Resolution 2.90 Å R-free 0.265
4U26 Crystal structure of the E. coli ribosome bound to dalfopristin and quinupristin. Deposited 2014-07-16	Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 54-meric	Chain BY 1–63（63 aa）	Not recorded	MG MAGNESIUM ION × 271 DOL 5-(2-DIETHYLAMINO-ETHANESULFONYL)-21-HYDROXY-10-ISOPROPYL-11,19-DIMETHYL-9,26-DIOXA-3,15,28-TRIAZA-TRICYCLO[23.2.1.00,255]OCTACOSA-1(27),12,17,19,25(28)-PENTAENE-2,8,14,23-TETRAONE × 1 ZN ZINC ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions MICROBATCH；pH 6.5；291 K；PEG8k, MPD	Resolution 2.80 Å R-free 0.271
4U26 Crystal structure of the E. coli ribosome bound to dalfopristin and quinupristin. Deposited 2014-07-16	Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 53-meric	Chain DY 1–63（63 aa）	Not recorded	MG MAGNESIUM ION × 227 DOL 5-(2-DIETHYLAMINO-ETHANESULFONYL)-21-HYDROXY-10-ISOPROPYL-11,19-DIMETHYL-9,26-DIOXA-3,15,28-TRIAZA-TRICYCLO[23.2.1.00,255]OCTACOSA-1(27),12,17,19,25(28)-PENTAENE-2,8,14,23-TETRAONE × 1 ZN ZINC ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions MICROBATCH；pH 6.5；291 K；PEG8k, MPD	Resolution 2.80 Å R-free 0.271
4U27 Crystal structure of the E. coli ribosome bound to flopristin and linopristin. Deposited 2014-07-16	Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 54-meric	Chain BY 1–63（63 aa）	Not recorded	MG MAGNESIUM ION × 271 VIF Flopristin × 1 ZN ZINC ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions MICROBATCH；pH 6.5；291 K；PEG8k, MPD	Resolution 2.80 Å R-free 0.260
4U27 Crystal structure of the E. coli ribosome bound to flopristin and linopristin. Deposited 2014-07-16	Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 53-meric	Chain DY 1–63（63 aa）	Not recorded	MG MAGNESIUM ION × 227 VIF Flopristin × 1 ZN ZINC ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions MICROBATCH；pH 6.5；291 K；PEG8k, MPD	Resolution 2.80 Å R-free 0.260
4V47 Real space refined coordinates of the 30S and 50S subunits fitted into the low resolution cryo-EM map of the EF-G.GTP state of E. coli 70S ribosome Deposited 2003-05-06	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 43 PDB declaration: 46-meric	Chain AW 1–63（63 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer Tris；pH 7.5；Tris cryo-EM vitrification conditions Rapid-freezing in liquid ethane	Resolution 12.30 Å
4V48 Real space refined coordinates of the 30S and 50S subunits fitted into the low resolution cryo-EM map of the initiation-like state of E. coli 70S ribosome Deposited 2003-05-06	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 44 PDB declaration: 48-meric	Chain AW 1–63（63 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer hepes；pH 7.5；hepes cryo-EM vitrification conditions Rapid-freezing in liquid ethane	Resolution 11.50 Å
4V4H Crystal structure of the bacterial ribosome from Escherichia coli in complex with the antibiotic kasugamyin at 3.5A resolution. Deposited 2006-08-04	Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 52-meric	Chain BX 1–63（63 aa）	Not recorded	KSG (1S,2R,3S,4R,5S,6S)-2,3,4,5,6-PENTAHYDROXYCYCLOHEXYL 2-AMINO-4-{[CARBOXY(IMINO)METHYL]AMINO}-2,3,4,6-TETRADEOXY-ALPHA-D-ARABINO-HEXOPYRANOSIDE × 1 MG MAGNESIUM ION × 170	X-RAY DIFFRACTION X-ray crystallization conditions pH 7.5；MPD, PEG 8000, MGCL2, NH4CL, SPERMINE, SPERMIDINE, TRIS-HCL, EDTA, PH 7.5, BATCH, TEMPERATURE 277K, pH 7.50	Resolution 3.46 Å R-free 0.331
4V4H Crystal structure of the bacterial ribosome from Escherichia coli in complex with the antibiotic kasugamyin at 3.5A resolution. Deposited 2006-08-04	Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 52-meric	Chain DX 1–63（63 aa）	Not recorded	KSG (1S,2R,3S,4R,5S,6S)-2,3,4,5,6-PENTAHYDROXYCYCLOHEXYL 2-AMINO-4-{[CARBOXY(IMINO)METHYL]AMINO}-2,3,4,6-TETRADEOXY-ALPHA-D-ARABINO-HEXOPYRANOSIDE × 1 MG MAGNESIUM ION × 173	X-RAY DIFFRACTION X-ray crystallization conditions pH 7.5；MPD, PEG 8000, MGCL2, NH4CL, SPERMINE, SPERMIDINE, TRIS-HCL, EDTA, PH 7.5, BATCH, TEMPERATURE 277K, pH 7.50	Resolution 3.46 Å R-free 0.331
4V4Q Crystal structure of the bacterial ribosome from Escherichia coli at 3.5 A resolution. Deposited 2005-08-30	Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 52-meric	Chain BX 1–63（63 aa）	Not recorded	MG MAGNESIUM ION × 170	X-RAY DIFFRACTION X-ray crystallization conditions batch；pH 7.5；277 K；MPD, PEG 8000, MgCl2, NH4Cl, spermine, spermidine, TRIS, EDTA, pH 7.5, batch, temperature 277K	Resolution 3.46 Å R-free 0.331
4V4Q Crystal structure of the bacterial ribosome from Escherichia coli at 3.5 A resolution. Deposited 2005-08-30	Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 52-meric	Chain DX 1–63（63 aa）	Not recorded	MG MAGNESIUM ION × 173	X-RAY DIFFRACTION X-ray crystallization conditions batch；pH 7.5；277 K；MPD, PEG 8000, MgCl2, NH4Cl, spermine, spermidine, TRIS, EDTA, pH 7.5, batch, temperature 277K	Resolution 3.46 Å R-free 0.331
4V4V Structure of a pre-translocational E. coli ribosome obtained by fitting atomic models for RNA and protein components into cryo-EM map EMD-1056 Deposited 2006-05-09	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 46 PDB declaration: 52-meric	Chain BW 1–60（60 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE；rapid-freezing in liquid ethane	Resolution 15.00 Å
4V4W Structure of a SecM-stalled E. coli ribosome complex obtained by fitting atomic models for RNA and protein components into cryo-EM map EMD-1143 Deposited 2006-05-09	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 46 PDB declaration: 52-meric	Chain BW 1–60（60 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE；rapid-freezing in liquid ethane	Resolution 15.00 Å
4V50 Crystal Structure of Ribosome with messenger RNA and the Anticodon stem-loop of P-site tRNA. Deposited 2006-08-16	Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 54-meric	Chain BY 1–63（63 aa）	Not recorded	MG MAGNESIUM ION × 181 ZN ZINC ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions batch；pH 7.5；277 K；MPD, PEG 8000, MGCL2, NH4CL, SPERMINE, SPERMIDINE, TRIS, EDTA, pH 7.5, batch, temperature 277K	Resolution 3.22 Å R-free 0.320
4V50 Crystal Structure of Ribosome with messenger RNA and the Anticodon stem-loop of P-site tRNA. Deposited 2006-08-16	Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 54-meric	Chain DY 1–63（63 aa）	Not recorded	MG MAGNESIUM ION × 177 ZN ZINC ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions batch；pH 7.5；277 K；MPD, PEG 8000, MGCL2, NH4CL, SPERMINE, SPERMIDINE, TRIS, EDTA, pH 7.5, batch, temperature 277K	Resolution 3.22 Å R-free 0.320
4V52 Crystal structure of the bacterial ribosome from Escherichia coli in complex with neomycin. Deposited 2007-06-15	Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 52-meric	Chain BX 1–63（63 aa）	Not recorded	NMY NEOMYCIN × 2 MG MAGNESIUM ION × 170 ZN ZINC ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions batch；pH 7.5；277 K；MPD, PEG 8000, MgCl2, NH4Cl, spermine, spermidine, TRIS, EDTA, pH 7.5, batch, temperature 277K	Resolution 3.21 Å R-free 0.309
4V52 Crystal structure of the bacterial ribosome from Escherichia coli in complex with neomycin. Deposited 2007-06-15	Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 52-meric	Chain DX 1–63（63 aa）	Not recorded	NMY NEOMYCIN × 2 MG MAGNESIUM ION × 173 ZN ZINC ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions batch；pH 7.5；277 K；MPD, PEG 8000, MgCl2, NH4Cl, spermine, spermidine, TRIS, EDTA, pH 7.5, batch, temperature 277K	Resolution 3.21 Å R-free 0.309
4V53 Crystal structure of the bacterial ribosome from Escherichia coli in complex with gentamicin. Deposited 2007-06-16	Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 52-meric	Chain BX 1–63（63 aa）	Not recorded	MG MAGNESIUM ION × 170 LLL (2R,3R,4R,5R)-2-((1S,2S,3R,4S,6R)-4,6-DIAMINO-3-((2R,3R,6S)-3-AMINO-6-(AMINOMETHYL)-TETRAHYDRO-2H-PYRAN-2-YLOXY)-2-HYDR OXYCYCLOHEXYLOXY)-5-METHYL-4-(METHYLAMINO)-TETRAHYDRO-2H-PYRAN-3,5-DIOL × 4 ZN ZINC ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions batch；pH 7.5；277 K；MPD, PEG 8000, MgCl2, NH4Cl, spermine, spermidine, TRIS, EDTA, pH 7.5, batch, temperature 277K	Resolution 3.54 Å R-free 0.320
4V53 Crystal structure of the bacterial ribosome from Escherichia coli in complex with gentamicin. Deposited 2007-06-16	Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 52-meric	Chain DX 1–63（63 aa）	Not recorded	MG MAGNESIUM ION × 173 LLL (2R,3R,4R,5R)-2-((1S,2S,3R,4S,6R)-4,6-DIAMINO-3-((2R,3R,6S)-3-AMINO-6-(AMINOMETHYL)-TETRAHYDRO-2H-PYRAN-2-YLOXY)-2-HYDR OXYCYCLOHEXYLOXY)-5-METHYL-4-(METHYLAMINO)-TETRAHYDRO-2H-PYRAN-3,5-DIOL × 4 ZN ZINC ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions batch；pH 7.5；277 K；MPD, PEG 8000, MgCl2, NH4Cl, spermine, spermidine, TRIS, EDTA, pH 7.5, batch, temperature 277K	Resolution 3.54 Å R-free 0.320
4V54 Crystal structure of the bacterial ribosome from Escherichia coli in complex with ribosome recycling factor (RRF). Deposited 2007-06-16	Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 53-meric	Chain BX 1–63（63 aa）	Not recorded	MG MAGNESIUM ION × 170 ZN ZINC ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions batch；pH 7.5；277 K；MPD, PEG 8000, MgCl2, NH4Cl, spermine, spermidine, TRIS, EDTA, pH 7.5, batch, temperature 277K	Resolution 3.30 Å R-free 0.304
4V54 Crystal structure of the bacterial ribosome from Escherichia coli in complex with ribosome recycling factor (RRF). Deposited 2007-06-16	Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 53-meric	Chain DX 1–63（63 aa）	Not recorded	MG MAGNESIUM ION × 173 ZN ZINC ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions batch；pH 7.5；277 K；MPD, PEG 8000, MgCl2, NH4Cl, spermine, spermidine, TRIS, EDTA, pH 7.5, batch, temperature 277K	Resolution 3.30 Å R-free 0.304
4V55 Crystal structure of the bacterial ribosome from Escherichia coli in complex with gentamicin and ribosome recycling factor (RRF). Deposited 2007-06-17	Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 53-meric	Chain BX 1–63（63 aa）	Not recorded	MG MAGNESIUM ION × 170 LLL (2R,3R,4R,5R)-2-((1S,2S,3R,4S,6R)-4,6-DIAMINO-3-((2R,3R,6S)-3-AMINO-6-(AMINOMETHYL)-TETRAHYDRO-2H-PYRAN-2-YLOXY)-2-HYDR OXYCYCLOHEXYLOXY)-5-METHYL-4-(METHYLAMINO)-TETRAHYDRO-2H-PYRAN-3,5-DIOL × 2 ZN ZINC ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions batch；pH 7.5；277 K；MPD, PEG 8000, MgCl2, NH4Cl, spermine, spermidine, TRIS, EDTA, pH 7.5, batch, temperature 277K	Resolution 4.00 Å R-free 0.305
4V55 Crystal structure of the bacterial ribosome from Escherichia coli in complex with gentamicin and ribosome recycling factor (RRF). Deposited 2007-06-17	Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 53-meric	Chain DX 1–63（63 aa）	Not recorded	MG MAGNESIUM ION × 173 LLL (2R,3R,4R,5R)-2-((1S,2S,3R,4S,6R)-4,6-DIAMINO-3-((2R,3R,6S)-3-AMINO-6-(AMINOMETHYL)-TETRAHYDRO-2H-PYRAN-2-YLOXY)-2-HYDR OXYCYCLOHEXYLOXY)-5-METHYL-4-(METHYLAMINO)-TETRAHYDRO-2H-PYRAN-3,5-DIOL × 2 ZN ZINC ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions batch；pH 7.5；277 K；MPD, PEG 8000, MgCl2, NH4Cl, spermine, spermidine, TRIS, EDTA, pH 7.5, batch, temperature 277K	Resolution 4.00 Å R-free 0.305
4V56 Crystal structure of the bacterial ribosome from Escherichia coli in complex with spectinomycin. Deposited 2007-07-21	Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 52-meric	Chain BX 1–63（63 aa）	Not recorded	MG MAGNESIUM ION × 170 SCM SPECTINOMYCIN × 1 ZN ZINC ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions batch；pH 7.5；277 K；MPD, PEG 8000, MgCl2, NH4Cl, spermine, spermidine, TRIS, EDTA, pH 7.5, batch, temperature 277K	Resolution 3.93 Å R-free 0.311
4V56 Crystal structure of the bacterial ribosome from Escherichia coli in complex with spectinomycin. Deposited 2007-07-21	Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 52-meric	Chain DX 1–63（63 aa）	Not recorded	MG MAGNESIUM ION × 170 SCM SPECTINOMYCIN × 1 ZN ZINC ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions batch；pH 7.5；277 K；MPD, PEG 8000, MgCl2, NH4Cl, spermine, spermidine, TRIS, EDTA, pH 7.5, batch, temperature 277K	Resolution 3.93 Å R-free 0.311
4V57 Crystal structure of the bacterial ribosome from Escherichia coli in complex with spectinomycin and neomycin. Deposited 2007-07-21	Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 52-meric	Chain BX 1–63（63 aa）	Not recorded	NMY NEOMYCIN × 2 MG MAGNESIUM ION × 170 SCM SPECTINOMYCIN × 1 ZN ZINC ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions batch；pH 7.5；277 K；MPD, PEG 8000, MgCl2, NH4Cl, spermine, spermidine, TRIS, EDTA, pH 7.5, batch, temperature 277K	Resolution 3.50 Å R-free 0.306
4V57 Crystal structure of the bacterial ribosome from Escherichia coli in complex with spectinomycin and neomycin. Deposited 2007-07-21	Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 52-meric	Chain DX 1–63（63 aa）	Not recorded	NMY NEOMYCIN × 2 MG MAGNESIUM ION × 170 SCM SPECTINOMYCIN × 1 ZN ZINC ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions batch；pH 7.5；277 K；MPD, PEG 8000, MgCl2, NH4Cl, spermine, spermidine, TRIS, EDTA, pH 7.5, batch, temperature 277K	Resolution 3.50 Å R-free 0.306
4V5H E.Coli 70s Ribosome Stalled During Translation Of Tnac Leader Peptide. Deposited 2009-10-26	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 56-meric	Chain B1 1–63（63 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer 50 MM TRIS-HCL PH 8.0, 10MM MG-ACETATE, 50MM KCL, 10MM NH4CL, 2MM EGTA, 2MM L-TRYPTOPHAN, 10 MM DTT；pH 8；50 MM TRIS-HCL PH 8.0, 10MM MG-ACETATE, 50MM KCL, 10MM NH4CL, 2MM EGTA, 2MM L-TRYPTOPHAN, 10 MM DTT cryo-EM vitrification conditions Cryogen ETHANE；CRYOGEN- ETHANE,	Resolution 5.80 Å
4V5Y Crystal structure of the bacterial ribosome from Escherichia coli in complex with paromomycin and ribosome recycling factor (RRF). Deposited 2007-06-19	Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 53-meric	Chain BX 1–63（63 aa）	Not recorded	MG MAGNESIUM ION × 170 PAR PAROMOMYCIN × 2 ZN ZINC ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions batch；pH 7.5；277 K；MPD, PEG 8000, MgCl2, NH4Cl, spermine, spermidine, TRIS, EDTA, pH 7.5, batch, temperature 277K	Resolution 4.45 Å R-free 0.309
4V5Y Crystal structure of the bacterial ribosome from Escherichia coli in complex with paromomycin and ribosome recycling factor (RRF). Deposited 2007-06-19	Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 53-meric	Chain DX 1–63（63 aa）	Not recorded	MG MAGNESIUM ION × 173 PAR PAROMOMYCIN × 2 ZN ZINC ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions batch；pH 7.5；277 K；MPD, PEG 8000, MgCl2, NH4Cl, spermine, spermidine, TRIS, EDTA, pH 7.5, batch, temperature 277K	Resolution 4.45 Å R-free 0.309
4V64 Crystal structure of the bacterial ribosome from Escherichia coli in complex with hygromycin B. Deposited 2008-06-11	Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 52-meric	Chain BX 1–63（63 aa）	Not recorded	MG MAGNESIUM ION × 170 HYG HYGROMYCIN B × 1 ZN ZINC ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions batch；pH 7.5；277 K；MPD, PEG 8000, MgCl2, NH4Cl, spermine, spermidine, TRIS, EDTA, pH 7.5, batch, temperature 277K	Resolution 3.50 Å R-free 0.318
4V64 Crystal structure of the bacterial ribosome from Escherichia coli in complex with hygromycin B. Deposited 2008-06-11	Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 52-meric	Chain DX 1–63（63 aa）	Not recorded	MG MAGNESIUM ION × 173 HYG HYGROMYCIN B × 1 ZN ZINC ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions batch；pH 7.5；277 K；MPD, PEG 8000, MgCl2, NH4Cl, spermine, spermidine, TRIS, EDTA, pH 7.5, batch, temperature 277K	Resolution 3.50 Å R-free 0.318
4V65 Structure of the E. coli ribosome in the Pre-accommodation state Deposited 2008-08-03	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 57-meric	Chain BQ 1–63（63 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 9.00 Å
4V66 Structure of the E. coli ribosome and the tRNAs in Post-accommodation state Deposited 2008-08-03	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 57-meric	Chain BQ 1–63（63 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 9.00 Å
4V69 Ternary complex-bound E.coli 70S ribosome. Deposited 2008-12-11	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 58-meric	Chain BY 1–63（63 aa）	Not recorded	GDP GUANOSINE-5'-DIPHOSPHATE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE；Vitrification using FEI Vitrobot; blot 3 seconds before plunging with an offset of -1mm	Resolution 6.70 Å
4V6C Crystal structure of the E. coli 70S ribosome in an intermediate state of ratcheting Deposited 2009-06-27	Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 52-meric	Chain BY 1–63（63 aa）	Not recorded	MG MAGNESIUM ION × 184 ZN ZINC ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions MICROBATCH；291 K；4% PEG 8000, 4% MPD, 3.8 mM MgCl2, 380 mM NH4Cl, 5.5 mM putrescine, 5 mM spermidine, microbatch, temperature 291K	Resolution 3.19 Å R-free 0.252
4V6C Crystal structure of the E. coli 70S ribosome in an intermediate state of ratcheting Deposited 2009-06-27	Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 52-meric	Chain DY 1–63（63 aa）	Not recorded	MG MAGNESIUM ION × 179 ZN ZINC ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions MICROBATCH；291 K；4% PEG 8000, 4% MPD, 3.8 mM MgCl2, 380 mM NH4Cl, 5.5 mM putrescine, 5 mM spermidine, microbatch, temperature 291K	Resolution 3.19 Å R-free 0.252
4V6D Crystal structure of the E. coli 70S ribosome in an intermediate state of ratcheting Deposited 2009-06-27	Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 54-meric	Chain BY 1–63（63 aa）	Not recorded	MG MAGNESIUM ION × 184 ZN ZINC ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions MICROBATCH；pH 7；291 K；4% PEG 8000, 4% MPD, 3.8 mM MgCl2, 380 mM NH4Cl, 5.5 mM putrescine, 5 mM spermidine, pH 7.0, microbatch, temperature 291K	Resolution 3.81 Å R-free 0.253
4V6D Crystal structure of the E. coli 70S ribosome in an intermediate state of ratcheting Deposited 2009-06-27	Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 54-meric	Chain DY 1–63（63 aa）	Not recorded	MG MAGNESIUM ION × 179 ZN ZINC ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions MICROBATCH；pH 7；291 K；4% PEG 8000, 4% MPD, 3.8 mM MgCl2, 380 mM NH4Cl, 5.5 mM putrescine, 5 mM spermidine, pH 7.0, microbatch, temperature 291K	Resolution 3.81 Å R-free 0.253
4V6E Crystal structure of the E. coli 70S ribosome in an intermediate state of ratcheting Deposited 2009-06-28	Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric	Chain BY 1–63（63 aa）	Not recorded	MG MAGNESIUM ION × 184 ZN ZINC ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions MICROBATCH；pH 7；291 K；4% PEG 8000, 4% MPD, 3.8 mM MgCl2, 380 mM NH4Cl, 5.5 mM putrescine, 5 mM spermidine, pH 7.0, microbatch, temperature 291K	Resolution 3.71 Å R-free 0.268
4V6E Crystal structure of the E. coli 70S ribosome in an intermediate state of ratcheting Deposited 2009-06-28	Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric	Chain DY 1–63（63 aa）	Not recorded	MG MAGNESIUM ION × 179 ZN ZINC ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions MICROBATCH；pH 7；291 K；4% PEG 8000, 4% MPD, 3.8 mM MgCl2, 380 mM NH4Cl, 5.5 mM putrescine, 5 mM spermidine, pH 7.0, microbatch, temperature 291K	Resolution 3.71 Å R-free 0.268
4V6K Structural insights into cognate vs. near-cognate discrimination during decoding. Deposited 2011-01-07	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 58-meric	Chain AZ 1–63（63 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen NITROGEN	Resolution 8.25 Å
4V6L Structural insights into cognate vs. near-cognate discrimination during decoding. Deposited 2011-01-07	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 58-meric	Chain BZ 1–63（63 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen NITROGEN	Resolution 13.20 Å
4V6M Structure of the ribosome-SecYE complex in the membrane environment Deposited 2011-02-08	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 55 PDB declaration: 60-meric	Chain BY 1–63（63 aa）	Not recorded	PEV (1S)-2-{[(2-AMINOETHOXY)(HYDROXY)PHOSPHORYL]OXY}-1-[(PALMITOYLOXY)METHYL]ETHYL STEARATE × 101 PGV (1R)-2-{[{[(2S)-2,3-DIHYDROXYPROPYL]OXY}(HYDROXY)PHOSPHORYL]OXY}-1-[(PALMITOYLOXY)METHYL]ETHYL (11E)-OCTADEC-11-ENOATE × 32	ELECTRON MICROSCOPY cryo-EM buffer 20 mM Hepes (pH 7.2), 100 mM KOAc, 10 mM Mg(OAc)2, 1 mM DTT, 250 microg/ml chloramphenicol；pH 7.2；20 mM Hepes (pH 7.2), 100 mM KOAc, 10 mM Mg(OAc)2, 1 mM DTT, 250 microg/ml chloramphenicol cryo-EM vitrification conditions Cryogen ETHANE；liquid ethane was used as a cryogen	Resolution 7.10 Å
4V6N Structural characterization of mRNA-tRNA translocation intermediates (50S ribosome of class2 of the six classes) Deposited 2011-12-07	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 58-meric	Chain A0 1–63（63 aa）	Not recorded	FME N-FORMYLMETHIONINE × 1 TRP TRYPTOPHAN × 1	ELECTRON MICROSCOPY cryo-EM buffer HiFi buffer (50 mM Tris-HCl, pH 7.5, 70mM NH4Cl, 30 mM KCl, 3.5 mM MgCl2, 0.5 mM spermidine, 8mM putrescine, 2 mM DTT, 3.5 mM MgCl2)；pH 7.5；HiFi buffer (50 mM Tris-HCl, pH 7.5, 70mM NH4Cl, 30 mM KCl, 3.5 mM MgCl2, 0.5 mM spermidine, 8mM putrescine, 2 mM DTT, 3.5 mM MgCl2) cryo-EM vitrification conditions blot for 3 seconds；80 K；Cryogen NITROGEN；Blot for 3 seconds, plunge into liquid nitrogen (Vitrobot)	Resolution 12.10 Å
4V6O Structural characterization of mRNA-tRNA translocation intermediates (class 4a of the six classes) Deposited 2011-12-07	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 58-meric	Chain B0 1–63（63 aa）	Not recorded	TRP TRYPTOPHAN × 1 FME N-FORMYLMETHIONINE × 1	ELECTRON MICROSCOPY cryo-EM buffer HiFi buffer (50 mM Tris-HCl, pH 7.5, 70mM NH4Cl, 30 mM KCl, 3.5 mM MgCl2, 0.5 mM spermidine, 8mM putrescine, 2 mM DTT, 3.5 mM MgCl2)；pH 7.5；HiFi buffer (50 mM Tris-HCl, pH 7.5, 70mM NH4Cl, 30 mM KCl, 3.5 mM MgCl2, 0.5 mM spermidine, 8mM putrescine, 2 mM DTT, 3.5 mM MgCl2) cryo-EM vitrification conditions blot for 3 seconds；80 K；Cryogen NITROGEN；Blot for 3 seconds, plunge into liquid nitrogen (Vitrobot)	Resolution 14.70 Å
4V6P Structural characterization of mRNA-tRNA translocation intermediates (class 4b of the six classes) Deposited 2011-12-08	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 58-meric	Chain B0 1–63（63 aa）	Not recorded	TRP TRYPTOPHAN × 1 FME N-FORMYLMETHIONINE × 1	ELECTRON MICROSCOPY cryo-EM buffer HiFi buffer (50 mM Tris-HCl, pH 7.5, 70mM NH4Cl, 30 mM KCl, 3.5 mM MgCl2, 0.5 mM spermidine, 8mM putrescine, 2 mM DTT, 3.5 mM MgCl2)；pH 7.5；HiFi buffer (50 mM Tris-HCl, pH 7.5, 70mM NH4Cl, 30 mM KCl, 3.5 mM MgCl2, 0.5 mM spermidine, 8mM putrescine, 2 mM DTT, 3.5 mM MgCl2) cryo-EM vitrification conditions blot for 6 seconds；80 K；Cryogen NITROGEN；Blot for 6 seconds, plunge into liquid nitrogen (Vitrobot)	Resolution 13.50 Å
4V6Q Structural characterization of mRNA-tRNA translocation intermediates (class 5 of the six classes) Deposited 2011-12-08	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 58-meric	Chain B0 1–63（63 aa）	Not recorded	TRP TRYPTOPHAN × 1 FME N-FORMYLMETHIONINE × 1	ELECTRON MICROSCOPY cryo-EM buffer HiFi buffer (50 mM Tris-HCl, pH 7.5, 70mM NH4Cl, 30 mM KCl, 3.5 mM MgCl2, 0.5 mM spermidine, 8mM putrescine, 2 mM DTT, 3.5 mM MgCl2)；pH 7.5；HiFi buffer (50 mM Tris-HCl, pH 7.5, 70mM NH4Cl, 30 mM KCl, 3.5 mM MgCl2, 0.5 mM spermidine, 8mM putrescine, 2 mM DTT, 3.5 mM MgCl2) cryo-EM vitrification conditions blot for 3 seconds；80 K；Cryogen NITROGEN；Blot for 3 seconds, plunge into liquid nitrogen (Vitrobot)	Resolution 11.50 Å
4V6R Structural characterization of mRNA-tRNA translocation intermediates (class 6 of the six classes) Deposited 2011-12-08	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 58-meric	Chain B0 1–63（63 aa）	Not recorded	TRP TRYPTOPHAN × 1 FME N-FORMYLMETHIONINE × 1	ELECTRON MICROSCOPY cryo-EM buffer HiFi buffer (50 mM Tris-HCl, pH 7.5, 70mM NH4Cl, 30 mM KCl, 3.5 mM MgCl2, 0.5 mM spermidine, 8mM putrescine, 2 mM DTT, 3.5 mM MgCl2)；pH 7.5；HiFi buffer (50 mM Tris-HCl, pH 7.5, 70mM NH4Cl, 30 mM KCl, 3.5 mM MgCl2, 0.5 mM spermidine, 8mM putrescine, 2 mM DTT, 3.5 mM MgCl2) cryo-EM vitrification conditions blot for 3 seconds；80 K；Cryogen NITROGEN；Blot for 3 seconds, plunge into liquid nitrogen (Vitrobot)	Resolution 11.50 Å
4V6S Structural characterization of mRNA-tRNA translocation intermediates (class 3 of the six classes) Deposited 2011-12-09	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 57-meric	Chain A0 1–63（63 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer HiFi buffer (50 mM Tris-HCl, pH 7.5, 70mM NH4Cl, 30 mM KCl, 3.5 mM MgCl2, 0.5 mM spermidine, 8mM putrescine, 2 mM DTT, 3.5 mM MgCl2)；pH 7.5；HiFi buffer (50 mM Tris-HCl, pH 7.5, 70mM NH4Cl, 30 mM KCl, 3.5 mM MgCl2, 0.5 mM spermidine, 8mM putrescine, 2 mM DTT, 3.5 mM MgCl2) cryo-EM vitrification conditions blot for 3 seconds；80 K；Cryogen NITROGEN；Blot for 3 seconds, plunge into liquid nitrogen (Vitrobot)	Resolution 13.10 Å
4V6T Structure of the bacterial ribosome complexed by tmRNA-SmpB and EF-G during translocation and MLD-loading Deposited 2012-01-27	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 56-meric	Chain BY 1–63（63 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6 cryo-EM vitrification conditions blot for 5-10 seconds before plunging；96.15 K；Cryogen ETHANE；blot for 5-10 seconds before plunging into liquid ethane (FEI Vitrobot)	Resolution 8.30 Å
4V6V Tetracycline resistance protein Tet(O) bound to the ribosome Deposited 2013-02-25	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 53 PDB declaration: 58-meric	Chain B2 1–63（63 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM vitrification conditions Cryogen ETHANE	Resolution 9.80 Å
4V6Y E. coli 70S-fMetVal-tRNAVal-tRNAfMet complex in classic pre-translocation state (pre1a) Deposited 2013-10-14	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric	Chain BY 1–63（63 aa）	Not recorded	VAL VALINE × 1 FME N-FORMYLMETHIONINE × 1	ELECTRON MICROSCOPY cryo-EM buffer 50 mM Tris-HCl, 70 mM NH4Cl, 30 mM KCl, 7 mM MgCl2, 0.6 mM spermine, 0.4 mM spermidine；pH 7.5；50 mM Tris-HCl, 70 mM NH4Cl, 30 mM KCl, 7 mM MgCl2, 0.6 mM spermine, 0.4 mM spermidine cryo-EM vitrification conditions Manual blotting for about 2 seconds；Cryogen ETHANE；Manual blotting for about 2 seconds prior to plunging into liquid ethane (custom-built CEVS vitrification instrument with dew point temperature adjusted to 18 degrees C)	Resolution 12.00 Å
4V6Z E. coli 70S-fMetVal-tRNAVal-tRNAfMet complex in classic pre-translocation state (pre1b) Deposited 2013-10-14	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric	Chain BY 1–63（63 aa）	Not recorded	VAL VALINE × 1 FME N-FORMYLMETHIONINE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 12.00 Å
4V70 E. coli 70S-fMetVal-tRNAVal-tRNAfMet complex in intermediate pre-translocation state (pre3) Deposited 2013-10-14	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric	Chain BY 1–63（63 aa）	Not recorded	VAL VALINE × 1 FME N-FORMYLMETHIONINE × 1	ELECTRON MICROSCOPY cryo-EM buffer 50 mM Tris-HCl, 70 mM NH4Cl, 30 mM KCl, 7 mM MgCl2, 0.6 mM spermine, 0.4 mM spermidine；pH 7.5；50 mM Tris-HCl, 70 mM NH4Cl, 30 mM KCl, 7 mM MgCl2, 0.6 mM spermine, 0.4 mM spermidine cryo-EM vitrification conditions Manual blotting for about 2 seconds；Cryogen ETHANE；Manual blotting for about 2 seconds prior to plunging into liquid ethane (custom-built CEVS vitrification instrument with dew point temperature adjusted to 18 degrees C)	Resolution 17.00 Å
4V71 E. coli 70S-fMetVal-tRNAVal-tRNAfMet complex in intermediate pre-translocation state (pre2) Deposited 2013-10-14	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric	Chain BY 1–63（63 aa）	Not recorded	VAL VALINE × 1 FME N-FORMYLMETHIONINE × 1	ELECTRON MICROSCOPY cryo-EM buffer 50 mM Tris-HCl, 70 mM NH4Cl, 30 mM KCl, 7 mM MgCl2, 0.6 mM spermine, 0.4 mM spermidine；pH 7.5；50 mM Tris-HCl, 70 mM NH4Cl, 30 mM KCl, 7 mM MgCl2, 0.6 mM spermine, 0.4 mM spermidine cryo-EM vitrification conditions Manual blotting for about 2 seconds；Cryogen ETHANE；Manual blotting for about 2 seconds prior to plunging into liquid ethane (custom-built CEVS vitrification instrument with dew point temperature adjusted to 18 degrees C)	Resolution 20.00 Å
4V72 E. coli 70S-fMetVal-tRNAVal-tRNAfMet complex in hybrid pre-translocation state (pre4) Deposited 2013-10-14	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric	Chain BY 1–63（63 aa）	Not recorded	VAL VALINE × 1 FME N-FORMYLMETHIONINE × 1	ELECTRON MICROSCOPY cryo-EM buffer 50 mM Tris-HCl, 70 mM NH4Cl, 30 mM KCl, 7 mM MgCl2, 0.6 mM spermine, 0.4 mM spermidine；pH 7.5；50 mM Tris-HCl, 70 mM NH4Cl, 30 mM KCl, 7 mM MgCl2, 0.6 mM spermine, 0.4 mM spermidine cryo-EM vitrification conditions Manual blotting for about 2 seconds；Cryogen ETHANE；Manual blotting for about 2 seconds prior to plunging into liquid ethane (custom-built CEVS vitrification instrument with dew point temperature adjusted to 18 degrees C)	Resolution 13.00 Å
4V73 E. coli 70S-fMetVal-tRNAVal-tRNAfMet complex in hybrid pre-translocation state (pre5a) Deposited 2013-10-14	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric	Chain BY 1–63（63 aa）	Not recorded	VAL VALINE × 1 FME N-FORMYLMETHIONINE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 15.00 Å
4V74 70S-fMetVal-tRNAVal-tRNAfMet complex in hybrid pre-translocation state (pre5b) Deposited 2013-10-14	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric	Chain BY 1–63（63 aa）	Not recorded	VAL VALINE × 1 FME N-FORMYLMETHIONINE × 1	ELECTRON MICROSCOPY cryo-EM buffer 50 mM Tris-HCl, 70 mM NH4Cl, 30 mM KCl, 7 mM MgCl2, 0.6 mM spermine, 0.4 mM spermidine；pH 7.5；50 mM Tris-HCl, 70 mM NH4Cl, 30 mM KCl, 7 mM MgCl2, 0.6 mM spermine, 0.4 mM spermidine cryo-EM vitrification conditions Manual blotting for about 2 seconds；Cryogen ETHANE；Manual blotting for about 2 seconds prior to plunging into liquid ethane (custom-built CEVS vitrification instrument with dew point temperature adjusted to 18 degrees C)	Resolution 17.00 Å
4V75 E. coli 70S-fMetVal-tRNAVal-tRNAfMet complex in classic post-translocation state (post1) Deposited 2013-10-14	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric	Chain BY 1–63（63 aa）	Not recorded	VAL VALINE × 1 FME N-FORMYLMETHIONINE × 1	ELECTRON MICROSCOPY cryo-EM buffer 50 mM Tris-HCl, 70 mM NH4Cl, 30 mM KCl, 7 mM MgCl2, 0.6 mM spermine, 0.4 mM spermidine；pH 7.5；50 mM Tris-HCl, 70 mM NH4Cl, 30 mM KCl, 7 mM MgCl2, 0.6 mM spermine, 0.4 mM spermidine cryo-EM vitrification conditions Manual blotting for about 2 seconds；Cryogen ETHANE；Manual blotting for about 2 seconds prior to plunging into liquid ethane (custom-built CEVS vitrification instrument with dew point temperature adjusted to 18 degrees C)	Resolution 12.00 Å
4V76 E. coli 70S-fMetVal-tRNAVal-tRNAfMet complex in intermediate post-translocation state (post2a) Deposited 2013-10-14	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric	Chain BY 1–63（63 aa）	Not recorded	VAL VALINE × 1 FME N-FORMYLMETHIONINE × 1	ELECTRON MICROSCOPY cryo-EM buffer 50 mM Tris-HCl, 70 mM NH4Cl, 30 mM KCl, 7 mM MgCl2, 0.6 mM spermine, 0.4 mM spermidine；pH 7.5；50 mM Tris-HCl, 70 mM NH4Cl, 30 mM KCl, 7 mM MgCl2, 0.6 mM spermine, 0.4 mM spermidine cryo-EM vitrification conditions Manual blotting for about 2 seconds；Cryogen ETHANE；Manual blotting for about 2 seconds prior to plunging into liquid ethane (custom-built CEVS vitrification instrument with dew point temperature adjusted to 18 degrees C)	Resolution 17.00 Å
4V77 E. coli 70S-fMetVal-tRNAVal-tRNAfMet complex in intermediate post-translocation state (post2b) Deposited 2013-10-14	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric	Chain BY 1–63（63 aa）	Not recorded	VAL VALINE × 1 FME N-FORMYLMETHIONINE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 17.00 Å
4V78 E. coli 70S-fMetVal-tRNAVal-tRNAfMet complex in intermediate post-translocation state (post3a) Deposited 2013-10-14	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric	Chain BY 1–63（63 aa）	Not recorded	VAL VALINE × 1 FME N-FORMYLMETHIONINE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 20.00 Å
4V79 E. coli 70S-fMetVal-tRNAVal-tRNAfMet complex in intermediate post-translocation state (post3b) Deposited 2013-10-14	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric	Chain BY 1–63（63 aa）	Not recorded	VAL VALINE × 1 FME N-FORMYLMETHIONINE × 1	ELECTRON MICROSCOPY cryo-EM buffer 50 mM Tris-HCl, 70 mM NH4Cl, 30 mM KCl, 7 mM MgCl2, 0.6 mM spermine, 0.4 mM spermidine；pH 7.5；50 mM Tris-HCl, 70 mM NH4Cl, 30 mM KCl, 7 mM MgCl2, 0.6 mM spermine, 0.4 mM spermidine cryo-EM vitrification conditions Manual blotting for about 2 seconds；Cryogen ETHANE；Manual blotting for about 2 seconds prior to plunging into liquid ethane (custom-built CEVS vitrification instrument with dew point temperature adjusted to 18 degrees C)	Resolution 15.00 Å
4V7A E. coli 70S-fMetVal-tRNAVal post-translocation complex (post4) Deposited 2013-10-14	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric	Chain BY 1–63（63 aa）	Not recorded	VAL VALINE × 1 FME N-FORMYLMETHIONINE × 1	ELECTRON MICROSCOPY cryo-EM buffer 50 mM Tris-HCl, 70 mM NH4Cl, 30 mM KCl, 7 mM MgCl2, 0.6 mM spermine, 0.4 mM spermidine；pH 7.5；50 mM Tris-HCl, 70 mM NH4Cl, 30 mM KCl, 7 mM MgCl2, 0.6 mM spermine, 0.4 mM spermidine cryo-EM vitrification conditions Manual blotting for about 2 seconds；Cryogen ETHANE；Manual blotting for about 2 seconds prior to plunging into liquid ethane (custom-built CEVS vitrification instrument with dew point temperature adjusted to 18 degrees C)	Resolution 9.00 Å
4V7B Visualization of two tRNAs trapped in transit during EF-G-mediated translocation Deposited 2013-10-27	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 58-meric	Chain BY 1–63（63 aa）	Not recorded	FUA FUSIDIC ACID × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1	ELECTRON MICROSCOPY cryo-EM buffer 80 mM HEPES potassium, 75 mM NH4Cl, 10 mM MgCl2, 6 mM BME；pH 7.6；80 mM HEPES potassium, 75 mM NH4Cl, 10 mM MgCl2, 6 mM BME cryo-EM vitrification conditions blot for 5-10 seconds before plunging；96 K；Cryogen ETHANE；Blot for 5-10 seconds before plunging into liquid ethane (FEI VITROBOT MARK I)	Resolution 6.80 Å
4V7C Structure of the Ribosome with Elongation Factor G Trapped in the Pre-Translocation State (pre-translocation 70S*tRNA structure) Deposited 2013-11-20	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 58-meric	Chain B1 1–63（63 aa）	Not recorded	ZN ZINC ION × 1	ELECTRON MICROSCOPY cryo-EM buffer Polymix buffer；pH 7.6；10 mM HEPES-KOH, 5 mM MgCl2, 90 mM NH4Cl, 2 mM spermidine, 0.1 mM spermine, 6 mM BME, 0.5 mM viomycin, 0.5 mM GTP, 0.5 mM fusidic acid cryo-EM vitrification conditions Freshly glow-disharged grids were loaded into an FEI Mark II Vitrobot and equilibrated to 95% relative humidity at 22 degrees Celsius. 2 microliters of sample was applied through the side port, blotted for 7 seconds with a positional offset of 2, and plunged into liquid ethane.；Cryogen ETHANE；Freshly glow-disharged grids were loaded into an FEI Mark II Vitrobot and equilibrated to 95% relative humidity at 22 degrees Celsius. 2 microliters of sample was applied through the side port, blotted for 7 seconds with a positional offset of 2, and plunged into liquid ethane.	Resolution 7.60 Å
4V7D Structure of the Ribosome with Elongation Factor G Trapped in the Pre-Translocation State (pre-translocation 70StRNAEF-G structure) Deposited 2013-11-21	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 54 PDB declaration: 60-meric	Chain A2 1–63（63 aa）	Not recorded	ZN ZINC ION × 1	ELECTRON MICROSCOPY cryo-EM buffer Polymix buffer；pH 7.6；10 mM HEPES-KOH, 5 mM MgCl2, 90 mM NH4Cl, 2 mM spermidine, 0.1 mM spermine, 6 mM BME, 0.5 mM viomycin, 0.5 mM GTP, 0.5 mM fusidic acid cryo-EM vitrification conditions Freshly glow-disharged grids were loaded into an FEI Mark II Vitrobot and equilibrated to 95% relative humidity at 22 degrees Celsius. 2 microliters of sample was applied through the side port, blotted for 7 seconds with a positional offset of 2, and plunged into liquid ethane.；Cryogen ETHANE；Freshly glow-disharged grids were loaded into an FEI Mark II Vitrobot and equilibrated to 95% relative humidity at 22 degrees Celsius. 2 microliters of sample was applied through the side port, blotted for 7 seconds with a positional offset of 2, and plunged into liquid ethane.	Resolution 7.60 Å
4V7I Ribosome-SecY complex. Deposited 2009-10-21	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 53 PDB declaration: 56-meric	Chain AY 1–63（63 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer 50 MM HEPES- KOH PH 7.5, 100 MM KOAC, 10 MM MG(OAC)2, 0.05% DDM；pH 7.5；50 MM HEPES- KOH PH 7.5, 100 MM KOAC, 10 MM MG(OAC)2, 0.05% DDM cryo-EM vitrification conditions Cryogen ETHANE；THE SPECIMENS WERE PLUNGE FROZEN IN LIQUID ETHANE AT 4 DEGREES C AT AN RH OF ~90-95%.	Resolution 9.60 Å
4V7S Crystal structure of the E. coli ribosome bound to telithromycin. Deposited 2010-08-05	Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 52-meric	Chain BY 1–63（63 aa）	Not recorded	MG MAGNESIUM ION × 181 TEL TELITHROMYCIN × 1 ZN ZINC ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions MICROBATCH；pH 6.5；291 K；PEG8k, MPD, pH 6.5, microbatch, temperature 291K	Resolution 3.25 Å R-free 0.245
4V7S Crystal structure of the E. coli ribosome bound to telithromycin. Deposited 2010-08-05	Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 52-meric	Chain DY 1–63（63 aa）	Not recorded	MG MAGNESIUM ION × 179 ZN ZINC ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions MICROBATCH；pH 6.5；291 K；PEG8k, MPD, pH 6.5, microbatch, temperature 291K	Resolution 3.25 Å R-free 0.245
4V7T Crystal structure of the E. coli ribosome bound to chloramphenicol. Deposited 2010-08-14	Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 52-meric	Chain BY 1–63（63 aa）	Not recorded	MG MAGNESIUM ION × 183 CLM CHLORAMPHENICOL × 1 ZN ZINC ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions MICROBATCH；pH 6.5；291 K；PEG8k, MPD, pH 6.5, microbatch, temperature 291K	Resolution 3.19 Å R-free 0.252
4V7T Crystal structure of the E. coli ribosome bound to chloramphenicol. Deposited 2010-08-14	Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 52-meric	Chain DY 1–63（63 aa）	Not recorded	MG MAGNESIUM ION × 179 ZN ZINC ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions MICROBATCH；pH 6.5；291 K；PEG8k, MPD, pH 6.5, microbatch, temperature 291K	Resolution 3.19 Å R-free 0.252
4V7U Crystal structure of the E. coli ribosome bound to erythromycin. Deposited 2010-08-15	Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 52-meric	Chain BY 1–63（63 aa）	Not recorded	MG MAGNESIUM ION × 182 ERY ERYTHROMYCIN A × 1 ZN ZINC ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions MICROBATCH；pH 6.5；291 K；4.3% PEG 8000, 4.9% MPD, 3.8 mM MgCl2, 380 mM NH4Cl, 5.5 mM putrescine, 5 mM spermidine, 10 mM Tris, 20 mM MES pH 6.5. 0.25 mM EDTA, microbatch, temperature 291K	Resolution 3.10 Å R-free 0.254
4V7U Crystal structure of the E. coli ribosome bound to erythromycin. Deposited 2010-08-15	Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 52-meric	Chain DY 1–63（63 aa）	Not recorded	MG MAGNESIUM ION × 179 ZN ZINC ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions MICROBATCH；pH 6.5；291 K；4.3% PEG 8000, 4.9% MPD, 3.8 mM MgCl2, 380 mM NH4Cl, 5.5 mM putrescine, 5 mM spermidine, 10 mM Tris, 20 mM MES pH 6.5. 0.25 mM EDTA, microbatch, temperature 291K	Resolution 3.10 Å R-free 0.254
4V7V Crystal structure of the E. coli ribosome bound to clindamycin. Deposited 2010-08-16	Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 52-meric	Chain BY 1–63（63 aa）	Not recorded	MG MAGNESIUM ION × 182 CLY CLINDAMYCIN × 1 ZN ZINC ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions MICROBATCH；291 K；4.3% PEG 8000, 4.9% MPD, 10 mM Tris, 20 mM MES, pH 6.5-7.0, 3.8 mM MgCl2, 380 mM NH4Cl, 5.5 mM putrescine, 5 mM spermidine, 0.25 mM EDTA, microbatch, temperature 291K	Resolution 3.29 Å R-free 0.241
4V7V Crystal structure of the E. coli ribosome bound to clindamycin. Deposited 2010-08-16	Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 52-meric	Chain DY 1–63（63 aa）	Not recorded	MG MAGNESIUM ION × 179 ZN ZINC ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions MICROBATCH；291 K；4.3% PEG 8000, 4.9% MPD, 10 mM Tris, 20 mM MES, pH 6.5-7.0, 3.8 mM MgCl2, 380 mM NH4Cl, 5.5 mM putrescine, 5 mM spermidine, 0.25 mM EDTA, microbatch, temperature 291K	Resolution 3.29 Å R-free 0.241
4V89 Crystal Structure of Release Factor RF3 Trapped in the GTP State on a Rotated Conformation of the Ribosome (without viomycin) Deposited 2011-11-17	Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 54 PDB declaration: 58-meric	Chain B2 1–63（63 aa）	Not recorded	GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；295 K；Tris Ac PH.7.0 25-35 mM KCL 6.1% PEG 20000 %1 glycerol 50mM sucrose , VAPOR DIFFUSION, SITTING DROP, temperature 295K	Resolution 3.70 Å R-free 0.290
4V9C Allosteric control of the ribosome by small-molecule antibiotics Deposited 2012-07-25	Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 54-meric	Chain BY 1–63（63 aa）	Not recorded	MG MAGNESIUM ION × 224 NMY NEOMYCIN × 10 ZN ZINC ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions pH 6.5；291 K；PEG800, MPD, pH 6.5, microbatch, temperature 291K	Resolution 3.30 Å R-free 0.255
4V9C Allosteric control of the ribosome by small-molecule antibiotics Deposited 2012-07-25	Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric	Chain DY 1–63（63 aa）	Not recorded	MG MAGNESIUM ION × 266 NMY NEOMYCIN × 8 ZN ZINC ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions pH 6.5；291 K；PEG800, MPD, pH 6.5, microbatch, temperature 291K	Resolution 3.30 Å R-free 0.255
4V9D Structures of the bacterial ribosome in classical and hybrid states of tRNA binding Deposited 2012-07-31	Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric	Chain CY 1–63（63 aa）	Not recorded	MG MAGNESIUM ION × 271 ZN ZINC ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions MICROBATCH；pH 6.5；291 K；PEG8000, MPD, pH 6.5, microbatch, temperature 291K X-ray crystallization conditions MICROBATCH；pH 6.5；291 K；PEG8000, MPD, pH 6.5, microbatch, temperature 291K X-ray crystallization conditions MICROBATCH；pH 6.5；291 K；PEG8000, MPD, pH 6.5, microbatch, temperature 291K X-ray crystallization conditions MICROBATCH；pH 6.5；291 K；PEG8000, MPD, pH 6.5, microbatch, temperature 291K X-ray crystallization conditions MICROBATCH；pH 6.5；291 K；PEG8000, MPD, pH 6.5, microbatch, temperature 291K	Resolution 3.00 Å R-free 0.260
4V9D Structures of the bacterial ribosome in classical and hybrid states of tRNA binding Deposited 2012-07-31	Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 54-meric	Chain DY 1–63（63 aa）	Not recorded	MG MAGNESIUM ION × 227 ZN ZINC ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions MICROBATCH；pH 6.5；291 K；PEG8000, MPD, pH 6.5, microbatch, temperature 291K X-ray crystallization conditions MICROBATCH；pH 6.5；291 K；PEG8000, MPD, pH 6.5, microbatch, temperature 291K X-ray crystallization conditions MICROBATCH；pH 6.5；291 K；PEG8000, MPD, pH 6.5, microbatch, temperature 291K X-ray crystallization conditions MICROBATCH；pH 6.5；291 K；PEG8000, MPD, pH 6.5, microbatch, temperature 291K X-ray crystallization conditions MICROBATCH；pH 6.5；291 K；PEG8000, MPD, pH 6.5, microbatch, temperature 291K	Resolution 3.00 Å R-free 0.260
4V9O Control of ribosomal subunit rotation by elongation factor G Deposited 2013-05-03	Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 53 PDB declaration: 56-meric	Chain AY 1–63（63 aa）	Not recorded	MG MAGNESIUM ION × 187 ZN ZINC ION × 1 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1	X-RAY DIFFRACTION X-ray crystallization conditions MICROBATCH；pH 6.5；291 K；PEG8k, MPD, KSCN, pH 6.5, microbatch, temperature 291K X-ray crystallization conditions MICROBATCH；pH 6.5；291 K；PEG8k, MPD, KSCN, pH 6.5, microbatch, temperature 291K X-ray crystallization conditions MICROBATCH；pH 6.5；291 K；PEG8k, MPD, KSCN, pH 6.5, microbatch, temperature 291K X-ray crystallization conditions MICROBATCH；pH 6.5；291 K；PEG8k, KSCN, pH 6.5, microbatch, temperature 291K X-ray crystallization conditions MICROBATCH；pH 6.5；291 K；PEG8k, KSCN, pH 6.5, microbatch, temperature 291K	Resolution 2.90 Å R-free 0.272
4V9O Control of ribosomal subunit rotation by elongation factor G Deposited 2013-05-03	Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 2 Protein–RNA Heteromer；Protein × 52 PDB declaration: 55-meric	Chain CY 1–63（63 aa）	Not recorded	MG MAGNESIUM ION × 187 ZN ZINC ION × 1 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1	X-RAY DIFFRACTION X-ray crystallization conditions MICROBATCH；pH 6.5；291 K；PEG8k, MPD, KSCN, pH 6.5, microbatch, temperature 291K X-ray crystallization conditions MICROBATCH；pH 6.5；291 K；PEG8k, MPD, KSCN, pH 6.5, microbatch, temperature 291K X-ray crystallization conditions MICROBATCH；pH 6.5；291 K；PEG8k, MPD, KSCN, pH 6.5, microbatch, temperature 291K X-ray crystallization conditions MICROBATCH；pH 6.5；291 K；PEG8k, KSCN, pH 6.5, microbatch, temperature 291K X-ray crystallization conditions MICROBATCH；pH 6.5；291 K；PEG8k, KSCN, pH 6.5, microbatch, temperature 291K	Resolution 2.90 Å R-free 0.272
4V9O Control of ribosomal subunit rotation by elongation factor G Deposited 2013-05-03	Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 3 Protein–RNA Heteromer；Protein × 52 PDB declaration: 55-meric	Chain EY 1–63（63 aa）	Not recorded	MG MAGNESIUM ION × 187 ZN ZINC ION × 1 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1	X-RAY DIFFRACTION X-ray crystallization conditions MICROBATCH；pH 6.5；291 K；PEG8k, MPD, KSCN, pH 6.5, microbatch, temperature 291K X-ray crystallization conditions MICROBATCH；pH 6.5；291 K；PEG8k, MPD, KSCN, pH 6.5, microbatch, temperature 291K X-ray crystallization conditions MICROBATCH；pH 6.5；291 K；PEG8k, MPD, KSCN, pH 6.5, microbatch, temperature 291K X-ray crystallization conditions MICROBATCH；pH 6.5；291 K；PEG8k, KSCN, pH 6.5, microbatch, temperature 291K X-ray crystallization conditions MICROBATCH；pH 6.5；291 K；PEG8k, KSCN, pH 6.5, microbatch, temperature 291K	Resolution 2.90 Å R-free 0.272
4V9O Control of ribosomal subunit rotation by elongation factor G Deposited 2013-05-03	Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 4 Protein–RNA Heteromer；Protein × 51 PDB declaration: 54-meric	Chain GY 1–63（63 aa）	Not recorded	MG MAGNESIUM ION × 187 ZN ZINC ION × 1 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1	X-RAY DIFFRACTION X-ray crystallization conditions MICROBATCH；pH 6.5；291 K；PEG8k, MPD, KSCN, pH 6.5, microbatch, temperature 291K X-ray crystallization conditions MICROBATCH；pH 6.5；291 K；PEG8k, MPD, KSCN, pH 6.5, microbatch, temperature 291K X-ray crystallization conditions MICROBATCH；pH 6.5；291 K；PEG8k, MPD, KSCN, pH 6.5, microbatch, temperature 291K X-ray crystallization conditions MICROBATCH；pH 6.5；291 K；PEG8k, KSCN, pH 6.5, microbatch, temperature 291K X-ray crystallization conditions MICROBATCH；pH 6.5；291 K；PEG8k, KSCN, pH 6.5, microbatch, temperature 291K	Resolution 2.90 Å R-free 0.272
4V9P Control of ribosomal subunit rotation by elongation factor G Deposited 2013-05-03	Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 55-meric	Chain AY 1–63（63 aa）	Not recorded	MG MAGNESIUM ION × 185 ZN ZINC ION × 1 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1	X-RAY DIFFRACTION X-ray crystallization conditions MICROBATCH；pH 6.5；291 K；PEG8k, MPD, KSCN, pH 6.5, microbatch, temperature 291K X-ray crystallization conditions MICROBATCH；pH 6.5；291 K；PEG8k, MPD, KSCN, pH 6.5, microbatch, temperature 291K X-ray crystallization conditions MICROBATCH；pH 6.5；291 K；PEG8k, MPD, KSCN, pH 6.5, microbatch, temperature 291K X-ray crystallization conditions MICROBATCH；pH 6.5；291 K；PEG8k, KSCN, pH 6.5, microbatch, temperature 291K X-ray crystallization conditions MICROBATCH；pH 6.5；291 K；PEG8k, KSCN, pH 6.5, microbatch, temperature 291K	Resolution 2.90 Å R-free 0.267
4V9P Control of ribosomal subunit rotation by elongation factor G Deposited 2013-05-03	Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 2 Protein–RNA Heteromer；Protein × 51 PDB declaration: 54-meric	Chain CY 1–63（63 aa）	Not recorded	MG MAGNESIUM ION × 185 ZN ZINC ION × 1 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1	X-RAY DIFFRACTION X-ray crystallization conditions MICROBATCH；pH 6.5；291 K；PEG8k, MPD, KSCN, pH 6.5, microbatch, temperature 291K X-ray crystallization conditions MICROBATCH；pH 6.5；291 K；PEG8k, MPD, KSCN, pH 6.5, microbatch, temperature 291K X-ray crystallization conditions MICROBATCH；pH 6.5；291 K；PEG8k, MPD, KSCN, pH 6.5, microbatch, temperature 291K X-ray crystallization conditions MICROBATCH；pH 6.5；291 K；PEG8k, KSCN, pH 6.5, microbatch, temperature 291K X-ray crystallization conditions MICROBATCH；pH 6.5；291 K；PEG8k, KSCN, pH 6.5, microbatch, temperature 291K	Resolution 2.90 Å R-free 0.267
4V9P Control of ribosomal subunit rotation by elongation factor G Deposited 2013-05-03	Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 3 Protein–RNA Heteromer；Protein × 52 PDB declaration: 55-meric	Chain EY 1–63（63 aa）	Not recorded	MG MAGNESIUM ION × 185 ZN ZINC ION × 1 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1	X-RAY DIFFRACTION X-ray crystallization conditions MICROBATCH；pH 6.5；291 K；PEG8k, MPD, KSCN, pH 6.5, microbatch, temperature 291K X-ray crystallization conditions MICROBATCH；pH 6.5；291 K；PEG8k, MPD, KSCN, pH 6.5, microbatch, temperature 291K X-ray crystallization conditions MICROBATCH；pH 6.5；291 K；PEG8k, MPD, KSCN, pH 6.5, microbatch, temperature 291K X-ray crystallization conditions MICROBATCH；pH 6.5；291 K；PEG8k, KSCN, pH 6.5, microbatch, temperature 291K X-ray crystallization conditions MICROBATCH；pH 6.5；291 K；PEG8k, KSCN, pH 6.5, microbatch, temperature 291K	Resolution 2.90 Å R-free 0.267
4V9P Control of ribosomal subunit rotation by elongation factor G Deposited 2013-05-03	Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 4 Protein–RNA Heteromer；Protein × 50 PDB declaration: 53-meric	Chain GY 1–63（63 aa）	Not recorded	MG MAGNESIUM ION × 185 ZN ZINC ION × 1 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1	X-RAY DIFFRACTION X-ray crystallization conditions MICROBATCH；pH 6.5；291 K；PEG8k, MPD, KSCN, pH 6.5, microbatch, temperature 291K X-ray crystallization conditions MICROBATCH；pH 6.5；291 K；PEG8k, MPD, KSCN, pH 6.5, microbatch, temperature 291K X-ray crystallization conditions MICROBATCH；pH 6.5；291 K；PEG8k, MPD, KSCN, pH 6.5, microbatch, temperature 291K X-ray crystallization conditions MICROBATCH；pH 6.5；291 K；PEG8k, KSCN, pH 6.5, microbatch, temperature 291K X-ray crystallization conditions MICROBATCH；pH 6.5；291 K；PEG8k, KSCN, pH 6.5, microbatch, temperature 291K	Resolution 2.90 Å R-free 0.267
4WF1 Crystal structure of the E. coli ribosome bound to negamycin. Deposited 2014-09-11	Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 53-meric	Chain BY 1–63（63 aa）	Not recorded	MG MAGNESIUM ION × 271 ZN ZINC ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions MICROBATCH；pH 6.5；291 K；PEG8k, MPD	Resolution 3.09 Å R-free 0.244
4WF1 Crystal structure of the E. coli ribosome bound to negamycin. Deposited 2014-09-11	Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 52-meric	Chain DY 1–63（63 aa）	Not recorded	MG MAGNESIUM ION × 228 ZN ZINC ION × 1 NEG NEGAMYCIN × 1	X-RAY DIFFRACTION X-ray crystallization conditions MICROBATCH；pH 6.5；291 K；PEG8k, MPD	Resolution 3.09 Å R-free 0.244
4WOI 4,5-linked aminoglycoside antibiotics regulate the bacterial ribosome by targeting dynamic conformational processes within intersubunit bridge B2 Deposited 2014-10-15	Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 56-meric	Chain BY 1–63（63 aa）	Not recorded	MG MAGNESIUM ION × 269 PAR PAROMOMYCIN × 6 ZN ZINC ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions MICROBATCH；pH 6.5；291 K；PEG8k, MPD, KCl, KSCN	Resolution 3.00 Å R-free 0.253
4WOI 4,5-linked aminoglycoside antibiotics regulate the bacterial ribosome by targeting dynamic conformational processes within intersubunit bridge B2 Deposited 2014-10-15	Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 54-meric	Chain CY 1–63（63 aa）	Not recorded	MG MAGNESIUM ION × 226 PAR PAROMOMYCIN × 7 ZN ZINC ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions MICROBATCH；pH 6.5；291 K；PEG8k, MPD, KCl, KSCN	Resolution 3.00 Å R-free 0.253
4WWW Crystal structure of the E. coli ribosome bound to CEM-101 Deposited 2014-11-12	Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 52-meric	Chain RY 1–63（63 aa）	Not recorded	MG MAGNESIUM ION × 182 EM1 (3aS,4R,7S,9R,10R,11R,13R,15R,15aR)-1-{4-[4-(3-aminophenyl)-1H-1,2,3-triazol-1-yl]butyl}-4-ethyl-7-fluoro-11-methoxy-3a ,7,9,11,13,15-hexamethyl-2,6,8,14-tetraoxotetradecahydro-2H-oxacyclotetradecino[4,3-d][1,3]oxazol-10-yl 3,4,6-trideoxy-3-(dimethylamino)-beta-D-xylo-hexopyranoside × 1 ZN ZINC ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions MICROBATCH；pH 6.5；291 K；PEG8k, MPD	Resolution 3.10 Å R-free 0.252
4WWW Crystal structure of the E. coli ribosome bound to CEM-101 Deposited 2014-11-12	Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 52-meric	Chain YY 1–63（63 aa）	Not recorded	MG MAGNESIUM ION × 179 ZN ZINC ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions MICROBATCH；pH 6.5；291 K；PEG8k, MPD	Resolution 3.10 Å R-free 0.252
4YBB High-resolution structure of the Escherichia coli ribosome Deposited 2015-02-18	Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 53-meric	Chain DZ 2–63（62 aa）	Not recorded	MG MAGNESIUM ION × 263 PG4 TETRAETHYLENE GLYCOL × 6 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 14 PUT 1,4-DIAMINOBUTANE × 16 ZN ZINC ION × 2 PEG DI(HYDROXYETHYL)ETHER × 11 SPD SPERMIDINE × 4 1PE PENTAETHYLENE GLYCOL × 2 PGE TRIETHYLENE GLYCOL × 9 ACY ACETIC ACID × 3 EDO 1,2-ETHANEDIOL × 12 GUN GUANINE × 1 TRS 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL × 1	X-RAY DIFFRACTION X-ray crystallization conditions MICROBATCH；pH 6.5；291 K；PEG8k, MPD	Resolution 2.10 Å R-free 0.234
4YBB High-resolution structure of the Escherichia coli ribosome Deposited 2015-02-18	Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 52-meric	Chain CZ 2–63（62 aa）	Not recorded	MG MAGNESIUM ION × 200 PG4 TETRAETHYLENE GLYCOL × 1 ZN ZINC ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions MICROBATCH；pH 6.5；291 K；PEG8k, MPD	Resolution 2.10 Å R-free 0.234
5ADY Cryo-EM structures of the 50S ribosome subunit bound with HflX Deposited 2015-08-25	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 32 PDB declaration: 34-meric	Chain Y 1–63（63 aa）	Not recorded	GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1	ELECTRON MICROSCOPY cryo-EM buffer 20MM TRIS-HCL, 100MM NH4CL, 10MM MGCL2；pH 7.5；20MM TRIS-HCL, 100MM NH4CL, 10MM MGCL2 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 4.50 Å
5AFI 2.9A Structure of E. coli ribosome-EF-TU complex by cs-corrected cryo-EM Deposited 2015-01-22	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 59-meric	Chain Y 1–63（63 aa）	Not recorded	MG MAGNESIUM ION × 333 CL CHLORIDE ION × 2 FME N-FORMYLMETHIONINE × 1 KIR KIRROMYCIN × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 NA SODIUM ION × 2 ZN ZINC ION × 2	ELECTRON MICROSCOPY cryo-EM buffer 50MM HEPES-KOH, 70MMNH4CL, 30MM KCL, 20MM MGCL2, 1MM DTT, 0.6MM SPERMINE, 0.4MM SPERMIDINE, 0.15MM KIRROMYCIN；pH 7.5；50MM HEPES-KOH, 70MMNH4CL, 30MM KCL, 20MM MGCL2, 1MM DTT, 0.6MM SPERMINE, 0.4MM SPERMIDINE, 0.15MM KIRROMYCIN cryo-EM vitrification conditions Cryogen ETHANE；LIQUIDE ETHANE	Resolution 2.90 Å
5AKA EM structure of ribosome-SRP-FtsY complex in closed state Deposited 2015-03-03	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 31 PDB declaration: 34-meric	Chain X 1–63（63 aa）	Not recorded	MG MAGNESIUM ION × 110	ELECTRON MICROSCOPY cryo-EM buffer 50MM HEPES-KOH, 100MM KOAC, 8MM MG(OAC)2, 500UG/ML CHLORAMPHENICO；pH 7.5；50MM HEPES-KOH, 100MM KOAC, 8MM MG(OAC)2, 500UG/ML CHLORAMPHENICO cryo-EM vitrification conditions Cryogen ETHANE；LIQUID ETHAN	Resolution 5.70 Å
5GAD RNC-SRP-SR complex early state Deposited 2015-11-24	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 33 PDB declaration: 37-meric	Chain Z 1–63（63 aa）	Not recorded	MG MAGNESIUM ION × 433 ZN ZINC ION × 1 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.70 Å
5GAE RNC in complex with a translocating SecYEG Deposited 2015-11-25	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 33 PDB declaration: 36-meric	Chain Z 1–63（63 aa）	Not recorded	MG MAGNESIUM ION × 323 ZN ZINC ION × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.33 Å
5GAF RNC in complex with SRP Deposited 2015-11-25	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 32 PDB declaration: 36-meric	Chain Z 2–63（62 aa）	Not recorded	MG MAGNESIUM ION × 431 ZN ZINC ION × 1 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 4.30 Å
5GAG RNC in complex with SRP-SR in the closed state Deposited 2015-11-26	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 32 PDB declaration: 36-meric	Chain Z 1–63（63 aa）	Not recorded	MG MAGNESIUM ION × 431 ZN ZINC ION × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.80 Å
5GAH RNC in complex with SRP with detached NG domain Deposited 2015-11-26	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 32 PDB declaration: 36-meric	Chain Z 1–63（63 aa）	Not recorded	MG MAGNESIUM ION × 431 ZN ZINC ION × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.80 Å
5H5U Mechanistic insights into the alternative translation termination by ArfA and RF2 Deposited 2016-11-09	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 57-meric	Chain Z 1–63（63 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.01 Å
5IQR Structure of RelA bound to the 70S ribosome Deposited 2016-03-11	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 59-meric	Chain Y 1–63（63 aa）	Not recorded	MG MAGNESIUM ION × 299 ZN ZINC ION × 3 PAR PAROMOMYCIN × 1 MET METHIONINE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE；Grids were blotted for 5 s	Resolution 3.00 Å
5IT8 High-resolution structure of the Escherichia coli ribosome Deposited 2016-03-16	Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 53-meric	Chain DZ 2–63（62 aa）	Not recorded	MG MAGNESIUM ION × 266 PG4 TETRAETHYLENE GLYCOL × 6 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 14 PUT 1,4-DIAMINOBUTANE × 16 ZN ZINC ION × 2 PEG DI(HYDROXYETHYL)ETHER × 11 EDO 1,2-ETHANEDIOL × 12 PGE TRIETHYLENE GLYCOL × 9 SPD SPERMIDINE × 4 1PE PENTAETHYLENE GLYCOL × 2 ACY ACETIC ACID × 3 GUN GUANINE × 1 TRS 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL × 1	X-RAY DIFFRACTION X-ray crystallization conditions MICROBATCH；pH 6.5；291 K；PEG8k, MPD	Resolution 3.12 Å R-free 0.220
5IT8 High-resolution structure of the Escherichia coli ribosome Deposited 2016-03-16	Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 52-meric	Chain CZ 2–63（62 aa）	Not recorded	MG MAGNESIUM ION × 200 PG4 TETRAETHYLENE GLYCOL × 1 ZN ZINC ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions MICROBATCH；pH 6.5；291 K；PEG8k, MPD	Resolution 3.12 Å R-free 0.220
5J5B Structure of the WT E coli ribosome bound to tetracycline Deposited 2016-04-01	Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 53-meric	Chain DZ 2–63（62 aa）	Not recorded	MG MAGNESIUM ION × 267 PG4 TETRAETHYLENE GLYCOL × 6 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 14 PUT 1,4-DIAMINOBUTANE × 16 TAC TETRACYCLINE × 1 ZN ZINC ION × 2 PEG DI(HYDROXYETHYL)ETHER × 11 EDO 1,2-ETHANEDIOL × 12 PGE TRIETHYLENE GLYCOL × 9 SPD SPERMIDINE × 4 1PE PENTAETHYLENE GLYCOL × 2 ACY ACETIC ACID × 3 GUN GUANINE × 1 TRS 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL × 1	X-RAY DIFFRACTION X-ray crystallization conditions MICROBATCH；291 K；PEG 8000, Spermidine, Putrescine, Ammonium Chloride, Magnesium Chloride, MES pH 6.5	Resolution 2.80 Å R-free 0.219
5J5B Structure of the WT E coli ribosome bound to tetracycline Deposited 2016-04-01	Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 52-meric	Chain CZ 2–63（62 aa）	Not recorded	MG MAGNESIUM ION × 202 PG4 TETRAETHYLENE GLYCOL × 1 TAC TETRACYCLINE × 1 ZN ZINC ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions MICROBATCH；291 K；PEG 8000, Spermidine, Putrescine, Ammonium Chloride, Magnesium Chloride, MES pH 6.5	Resolution 2.80 Å R-free 0.219
5J7L Structure of the 70S E coli ribosome with the U1052G mutation in the 16S rRNA bound to tetracycline Deposited 2016-04-06	Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 53-meric	Chain DZ 2–63（62 aa）	Not recorded	MG MAGNESIUM ION × 268 PG4 TETRAETHYLENE GLYCOL × 6 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 14 PUT 1,4-DIAMINOBUTANE × 16 TAC TETRACYCLINE × 2 ZN ZINC ION × 2 PEG DI(HYDROXYETHYL)ETHER × 11 EDO 1,2-ETHANEDIOL × 12 PGE TRIETHYLENE GLYCOL × 9 SPD SPERMIDINE × 4 1PE PENTAETHYLENE GLYCOL × 2 ACY ACETIC ACID × 3 GUN GUANINE × 1 TRS 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL × 1	X-RAY DIFFRACTION X-ray crystallization conditions MICROBATCH；291 K；PEG 8k, Spermidine, Putrescine, Ammonium Chloride, Magnesium Chloride, MES pH 6.5	Resolution 3.00 Å R-free 0.193
5J7L Structure of the 70S E coli ribosome with the U1052G mutation in the 16S rRNA bound to tetracycline Deposited 2016-04-06	Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 52-meric	Chain CZ 2–63（62 aa）	Not recorded	MG MAGNESIUM ION × 204 PG4 TETRAETHYLENE GLYCOL × 1 TAC TETRACYCLINE × 2 ZN ZINC ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions MICROBATCH；291 K；PEG 8k, Spermidine, Putrescine, Ammonium Chloride, Magnesium Chloride, MES pH 6.5	Resolution 3.00 Å R-free 0.193
5J88 Structure of the E coli 70S ribosome with the U1060A mutation in 16S rRNA Deposited 2016-04-07	Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 53-meric	Chain DZ 2–63（62 aa）	Not recorded	MG MAGNESIUM ION × 266 PG4 TETRAETHYLENE GLYCOL × 6 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 14 PUT 1,4-DIAMINOBUTANE × 16 ZN ZINC ION × 2 PEG DI(HYDROXYETHYL)ETHER × 11 EDO 1,2-ETHANEDIOL × 12 PGE TRIETHYLENE GLYCOL × 9 SPD SPERMIDINE × 4 1PE PENTAETHYLENE GLYCOL × 2 ACY ACETIC ACID × 3 GUN GUANINE × 1 TRS 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL × 1	X-RAY DIFFRACTION X-ray crystallization conditions MICROBATCH；291 K；PEG 8k, Spermidine, Putrescine, Ammonium Chloride, Magnesium Chloride, MES pH 6.5	Resolution 3.32 Å R-free 0.219
5J88 Structure of the E coli 70S ribosome with the U1060A mutation in 16S rRNA Deposited 2016-04-07	Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 52-meric	Chain CZ 2–63（62 aa）	Not recorded	MG MAGNESIUM ION × 200 PG4 TETRAETHYLENE GLYCOL × 1 ZN ZINC ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions MICROBATCH；291 K；PEG 8k, Spermidine, Putrescine, Ammonium Chloride, Magnesium Chloride, MES pH 6.5	Resolution 3.32 Å R-free 0.219
5J8A Structure of the E coli 70S ribosome with the U1052G mutation in 16S rRNA bound to tigecycline Deposited 2016-04-07	Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 53-meric	Chain DZ 2–63（62 aa）	Not recorded	MG MAGNESIUM ION × 268 PG4 TETRAETHYLENE GLYCOL × 6 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 14 PUT 1,4-DIAMINOBUTANE × 16 T1C TIGECYCLINE × 1 ZN ZINC ION × 2 PEG DI(HYDROXYETHYL)ETHER × 11 EDO 1,2-ETHANEDIOL × 12 PGE TRIETHYLENE GLYCOL × 9 SPD SPERMIDINE × 4 1PE PENTAETHYLENE GLYCOL × 2 ACY ACETIC ACID × 3 GUN GUANINE × 1 TRS 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL × 1	X-RAY DIFFRACTION X-ray crystallization conditions MICROBATCH；291 K；PEG 8k, Spermidine, Putrescine, Ammonium Chloride, MAgnesium Chloride, MPD, MES pH 6.5	Resolution 3.10 Å R-free 0.192
5J8A Structure of the E coli 70S ribosome with the U1052G mutation in 16S rRNA bound to tigecycline Deposited 2016-04-07	Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 52-meric	Chain CZ 2–63（62 aa）	Not recorded	MG MAGNESIUM ION × 204 PG4 TETRAETHYLENE GLYCOL × 1 T1C TIGECYCLINE × 1 ZN ZINC ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions MICROBATCH；291 K；PEG 8k, Spermidine, Putrescine, Ammonium Chloride, MAgnesium Chloride, MPD, MES pH 6.5	Resolution 3.10 Å R-free 0.192
5J91 Structure of the Wild-type 70S E coli ribosome bound to Tigecycline Deposited 2016-04-08	Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 53-meric	Chain DZ 2–63（62 aa）	Not recorded	MG MAGNESIUM ION × 267 PG4 TETRAETHYLENE GLYCOL × 6 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 14 PUT 1,4-DIAMINOBUTANE × 16 T1C TIGECYCLINE × 1 ZN ZINC ION × 2 PEG DI(HYDROXYETHYL)ETHER × 11 EDO 1,2-ETHANEDIOL × 12 PGE TRIETHYLENE GLYCOL × 9 SPD SPERMIDINE × 4 1PE PENTAETHYLENE GLYCOL × 2 ACY ACETIC ACID × 3 GUN GUANINE × 1 TRS 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL × 1	X-RAY DIFFRACTION X-ray crystallization conditions MICROBATCH；pH 6.5；291 K；PEG 8k, Spermidine, Putrescine, Ammonium Chloride, MAgnesium Chloride, MPD, MES pH 6.5	Resolution 2.96 Å R-free 0.221
5J91 Structure of the Wild-type 70S E coli ribosome bound to Tigecycline Deposited 2016-04-08	Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 52-meric	Chain CZ 2–63（62 aa）	Not recorded	MG MAGNESIUM ION × 202 PG4 TETRAETHYLENE GLYCOL × 1 T1C TIGECYCLINE × 1 ZN ZINC ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions MICROBATCH；pH 6.5；291 K；PEG 8k, Spermidine, Putrescine, Ammonium Chloride, MAgnesium Chloride, MPD, MES pH 6.5	Resolution 2.96 Å R-free 0.221
5JC9 Structure of the Escherichia coli ribosome with the U1052G mutation in the 16S rRNA Deposited 2016-04-14	Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 53-meric	Chain DZ 2–63（62 aa）	Not recorded	MG MAGNESIUM ION × 267 PG4 TETRAETHYLENE GLYCOL × 6 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 14 PUT 1,4-DIAMINOBUTANE × 16 ZN ZINC ION × 2 PEG DI(HYDROXYETHYL)ETHER × 11 EDO 1,2-ETHANEDIOL × 12 PGE TRIETHYLENE GLYCOL × 9 SPD SPERMIDINE × 4 1PE PENTAETHYLENE GLYCOL × 2 ACY ACETIC ACID × 3 GUN GUANINE × 1 TRS 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL × 1	X-RAY DIFFRACTION X-ray crystallization conditions MICROBATCH；pH 6.5；291 K；PEG 8k, Spermidine, Putrescine, Ammonium Chloride, MAgnesium Chloride, MPD, MES	Resolution 3.03 Å R-free 0.200
5JC9 Structure of the Escherichia coli ribosome with the U1052G mutation in the 16S rRNA Deposited 2016-04-14	Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 52-meric	Chain CZ 2–63（62 aa）	Not recorded	MG MAGNESIUM ION × 202 PG4 TETRAETHYLENE GLYCOL × 1 ZN ZINC ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions MICROBATCH；pH 6.5；291 K；PEG 8k, Spermidine, Putrescine, Ammonium Chloride, MAgnesium Chloride, MPD, MES	Resolution 3.03 Å R-free 0.200
5JTE Cryo-EM structure of an ErmBL-stalled ribosome in complex with A-, P-, and E-tRNA Deposited 2016-05-09	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 58-meric	Chain BY 1–63（63 aa）	Not recorded	LYS LYSINE × 1 ERY ERYTHROMYCIN A × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.60 Å
5JU8 Cryo-EM structure of an ErmBL-stalled ribosome in complex with P-, and E-tRNA Deposited 2016-05-10	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric	Chain BY 1–63（63 aa）	Not recorded	ERY ERYTHROMYCIN A × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.60 Å
5KCR Cryo-EM structure of the Escherichia coli 70S ribosome in complex with antibiotic Avilamycin C, mRNA and P-site tRNA at 3.6A resolution Deposited 2016-06-06	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric	Chain 12 1–63（63 aa）	Not recorded	6UQ (2R,3S,4R,6S)-4-hydroxy-6-{[(2R,3aR,4R,4'R,5'S,6S,6'R,7aR)-4'-hydroxy-6-{[(2S,3R,4R,5S,6R)-3-hydroxy-2-{[(2R,3S,4S,5S,6S)-4-hydroxy-6-({(2R,3aS,3a'R,6S,6'R,7R,7'R,7aR,7a'R)-7'-hydroxy-7'-[(1S)-1-hydroxyethyl]-6'-methyl-7-[(2-methylpropanoyl)oxy]octahydro-4H-2,4'-spirobi[[1,3]dioxolo[4,5-c]pyran]-6-yl}oxy)-5-methoxy-2-(methoxymethyl)tetrahydro-2H-pyran-3-yl]oxy}-5-methoxy-6-methyltetrahydro-2H-pyran-4-yl]oxy}-4,6',7a-trimethyloctahydro-4H-spiro[1,3-dioxolo[4,5-c]pyran-2,2'-pyran]-5'-yl]oxy}-2-methyltetrahydro-2H-pyran-3-yl 3,5-dichloro-4-hydroxy-2-methoxy-6-methylbenzoate (non-preferred name) × 1 ZN ZINC ION × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE X-ray crystallization conditions pH 7.4	Resolution 3.60 Å
5KCS Cryo-EM structure of the Escherichia coli 70S ribosome in complex with antibiotic Evernimycin, mRNA, TetM and P-site tRNA at 3.9A resolution Deposited 2016-06-06	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 53 PDB declaration: 58-meric	Chain 12 1–63（63 aa）	Not recorded	EVN (2R,3R,4R,6S)-6-{[(2R,3aR,4R,4'R,5'S,6S,6'R,7S,7aR)-6-{[(2S,3R,4R,5S,6R)-2-{[(2R,3S,4S,5S,6S)-6-({(2R,3aS,3a'R,6S,7R,7' R,7aS,7a'S)-7'-[(2,4-dihydroxy-6-methylbenzoyl)oxy]-7-hydroxyoctahydro-4H-2,4'-spirobi[[1,3]dioxolo[4,5-c]pyran]-6-yl}ox y)-4-hydroxy-5-methoxy-2-(methoxymethyl)tetrahydro-2H-pyran-3-yl]oxy}-3-hydroxy-5-methoxy-6-methyltetrahydro-2H-pyran-4- yl]oxy}-4',7-dihydroxy-4,6',7a-trimethyloctahydro-4H-spiro[1,3-dioxolo[4,5-c]pyran-2,2'-pyran]-5'-yl]oxy}-4-{[(2R,4S,5R, 6S)-5-methoxy-4,6-dimethyl-4-nitrotetrahydro-2H-pyran-2-yl]oxy}-2-methyltetrahydro-2H-pyran-3-yl 3,5-dichloro-4-hydroxy-2-methoxy-6-methylbenzoate (non-preferred name) × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.90 Å
5KPS Structure of RelA bound to ribosome in absence of A/R tRNA (Structure I) Deposited 2016-07-05	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 58-meric	Chain Y 1–63（63 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE；Apply 2 uL sample and blot 4 seconds before plunging into liquid ethane (GATAN CRYOPLUNGE 3).	Resolution 3.90 Å
5KPV Structure of RelA bound to ribosome in presence of A/R tRNA (Structure II) Deposited 2016-07-05	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 59-meric	Chain X 1–63（63 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE；Apply 2 uL sample and blot 4 seconds before plunging into liquid ethane (GATAN CRYOPLUNGE 3).	Resolution 4.10 Å
5KPW Structure of RelA bound to ribosome in presence of A/R tRNA (Structure III) Deposited 2016-07-05	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 59-meric	Chain X 1–63（63 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE；Apply 2 uL sample and blot 4 seconds before plunging into liquid ethane (GATAN CRYOPLUNGE 3).	Resolution 3.90 Å
5KPX Structure of RelA bound to ribosome in presence of A/R tRNA (Structure IV) Deposited 2016-07-05	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 59-meric	Chain X 1–63（63 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE；Apply 2 uL sample and blot 4 seconds before plunging into liquid ethane (GATAN CRYOPLUNGE 3).	Resolution 3.90 Å
5L3P Cryo-EM structure of stringent response factor RelA bound to ErmCL-stalled ribosome complex Deposited 2016-05-24	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 58-meric	Chain 2 1–63（63 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.70 Å
5LZA Structure of the 70S ribosome with SECIS-mRNA and P-site tRNA (Initial complex, IC) Deposited 2016-09-29	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric	Chain Y 1–63（63 aa）	Not recorded	ZN ZINC ION × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5；50 mM Hepes-KOH, pH 7.5, 70 mM NH4Cl, 30 mM KCl, 7 mM MgCl2, 0.6mM spermine, 0.4mM spermidine, 2 mM DTT cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.60 Å
5LZB Structure of SelB-Sec-tRNASec bound to the 70S ribosome in the initial binding state (IB) Deposited 2016-09-29	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 58-meric	Chain Y 1–63（63 aa）	Not recorded	FME N-FORMYLMETHIONINE × 1 SEC SELENOCYSTEINE × 1 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 ZN ZINC ION × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5；50 mM Hepes-KOH, pH 7.5, 70 mM NH4Cl, 30 mM KCl, 7 mM MgCl2, 0.6mM spermine, 0.4mM spermidine, 2 mM DTT cryo-EM vitrification conditions Cryogen ETHANE	Resolution 5.30 Å
5LZC Structure of SelB-Sec-tRNASec bound to the 70S ribosome in the codon reading state (CR) Deposited 2016-09-29	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 58-meric	Chain Y 1–63（63 aa）	Not recorded	FME N-FORMYLMETHIONINE × 1 SEC SELENOCYSTEINE × 1 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 ZN ZINC ION × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5；50 mM Hepes-KOH, pH 7.5, 70 mM NH4Cl, 30 mM KCl, 7 mM MgCl2, 0.6mM spermine, 0.4mM spermidine, 2 mM DTT cryo-EM vitrification conditions Cryogen ETHANE	Resolution 4.80 Å
5LZD Structure of SelB-Sec-tRNASec bound to the 70S ribosome in the GTPase activated state (GA) Deposited 2016-09-29	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 58-meric	Chain Y 1–63（63 aa）	Not recorded	CL CHLORIDE ION × 1 MG MAGNESIUM ION × 147 FME N-FORMYLMETHIONINE × 1 SEC SELENOCYSTEINE × 1 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 ZN ZINC ION × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5；50 mM Hepes-KOH, pH 7.5, 70 mM NH4Cl, 30 mM KCl, 7 mM MgCl2, 0.6mM spermine, 0.4mM spermidine, 2 mM DTT cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.40 Å
5LZE Structure of the 70S ribosome with Sec-tRNASec in the classical pre-translocation state (C) Deposited 2016-09-29	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 57-meric	Chain Y 1–63（63 aa）	Not recorded	ZN ZINC ION × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5；50 mM Hepes-KOH, pH 7.5, 70 mM NH4Cl, 30 mM KCl, 7 mM MgCl2, 0.6 mM spermine, 0.4 mM spermidine, 2 mM DTT cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.50 Å
5LZF Structure of the 70S ribosome with fMetSec-tRNASec in the hybrid pre-translocation state (H) Deposited 2016-09-29	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric	Chain Y 1–63（63 aa）	Not recorded	ZN ZINC ION × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5；50 mM Hepes-KOH, pH 7.5, 70 mM NH4Cl, 30 mM KCl, 7 mM MgCl2, 0.6mM spermine, 0.4mM spermidine, 2 mM DTT cryo-EM vitrification conditions Cryogen ETHANE	Resolution 4.60 Å
5MDV Structure of ArfA and RF2 bound to the 70S ribosome (accommodated state) Deposited 2016-11-13	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 53 PDB declaration: 58-meric	Chain Y 1–63（63 aa）	Not recorded	MG MAGNESIUM ION × 440 FME N-FORMYLMETHIONINE × 1 ZN ZINC ION × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.97 Å
5MDW Structure of ArfA(A18T) and RF2 bound to the 70S ribosome (pre-accommodated state) Deposited 2016-11-13	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 53 PDB declaration: 58-meric	Chain Y 1–63（63 aa）	Not recorded	MG MAGNESIUM ION × 438 FME N-FORMYLMETHIONINE × 1 ZN ZINC ION × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.06 Å
5MDY Structure of ArfA and TtRF2 bound to the 70S ribosome (pre-accommodated state) Deposited 2016-11-13	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 53 PDB declaration: 62-meric	Chain Y 1–63（63 aa）	Not recorded	MG MAGNESIUM ION × 450 FME N-FORMYLMETHIONINE × 1 ZN ZINC ION × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.35 Å
5MDZ Structure of the 70S ribosome (empty A site) Deposited 2016-11-13	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 56-meric	Chain Y 1–63（63 aa）	Not recorded	MG MAGNESIUM ION × 437 FME N-FORMYLMETHIONINE × 1 ZN ZINC ION × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.10 Å
5MGP Structural basis for ArfA-RF2 mediated translation termination on stop-codon lacking mRNAs Deposited 2016-11-21	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 56-meric	Chain Y 1–63（63 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.2 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.10 Å
5NCO Quaternary complex between SRP, SR, and SecYEG bound to the translating ribosome Deposited 2017-03-06	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 36 PDB declaration: 40-meric	Chain Z 2–63（62 aa）	Not recorded	ZN ZINC ION × 1 ALF TETRAFLUOROALUMINATE ION × 2 MG MAGNESIUM ION × 2 GDP GUANOSINE-5'-DIPHOSPHATE × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 4.80 Å
5NP6 70S structure prior to bypassing Deposited 2017-04-13	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 57-meric	Chain w 1–63（63 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.60 Å
5NWY 2.9 A cryo-EM structure of VemP-stalled ribosome-nascent chain complex Deposited 2017-05-08	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 56-meric	Chain l 1–63（63 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.2 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.90 Å
5O2R Cryo-EM structure of the proline-rich antimicrobial peptide Api137 bound to the terminating ribosome Deposited 2017-05-22	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 53 PDB declaration: 58-meric	Chain Y 1–63（63 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.40 Å
5U4I Structural Basis of Co-translational Quality Control by ArfA and RF2 Bound to Ribosome Deposited 2016-12-04	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 57-meric	Chain Z 1–63（63 aa）	Not recorded	MG MAGNESIUM ION × 124 ZN ZINC ION × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.50 Å
5UYK 70S ribosome bound with cognate ternary complex not base-paired to A site codon (Structure I) Deposited 2017-02-24	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 53 PDB declaration: 60-meric	Chain 27 1–63（63 aa）	Not recorded	GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE；2 uL of complex was applied to each grid. After a 10-second incubation, the grids were blotted for 2 to 4 seconds.	Resolution 3.90 Å
5UYL 70S ribosome bound with cognate ternary complex base-paired to A site codon (Structure II) Deposited 2017-02-24	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 53 PDB declaration: 60-meric	Chain 27 1–63（63 aa）	Not recorded	FME N-FORMYLMETHIONINE × 1 PHE PHENYLALANINE × 1 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE；2 uL of complex was applied to each grid. After a 10-second incubation, the grids were blotted for 2 to 4 seconds.	Resolution 3.60 Å
5UYM 70S ribosome bound with cognate ternary complex base-paired to A site codon, closed 30S (Structure III) Deposited 2017-02-24	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 53 PDB declaration: 60-meric	Chain 27 1–63（63 aa）	Not recorded	MG MAGNESIUM ION × 383 ZN ZINC ION × 2 FME N-FORMYLMETHIONINE × 1 PHE PHENYLALANINE × 1 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE；2 uL of complex was applied to each grid. After a 10-second incubation, the grids were blotted for 2 to 4 seconds.	Resolution 3.20 Å
5UYN 70S ribosome bound with near-cognate ternary complex not base-paired to A site codon (Structure I-nc) Deposited 2017-02-24	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 53 PDB declaration: 60-meric	Chain 27 1–63（63 aa）	Not recorded	GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE；2 uL of complex was applied to each grid. After a 10-second incubation, the grids were blotted for 2 to 4 seconds.	Resolution 4.00 Å
5UYP 70S ribosome bound with near-cognate ternary complex base-paired to A site codon, open 30S (Structure II-nc) Deposited 2017-02-24	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 53 PDB declaration: 60-meric	Chain 27 1–63（63 aa）	Not recorded	FME N-FORMYLMETHIONINE × 1 LYS LYSINE × 1 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE；2 uL of complex was applied to each grid. After a 10-second incubation, the grids were blotted for 2 to 4 seconds.	Resolution 3.90 Å
5UYQ 70S ribosome bound with near-cognate ternary complex base-paired to A site codon, closed 30S (Structure III-nc) Deposited 2017-02-24	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 53 PDB declaration: 60-meric	Chain 27 1–63（63 aa）	Not recorded	FME N-FORMYLMETHIONINE × 1 LYS LYSINE × 1 MG MAGNESIUM ION × 1 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE；2 uL of complex was applied to each grid. After a 10-second incubation, the grids were blotted for 2 to 4 seconds.	Resolution 3.80 Å
5WDT 70S ribosome-EF-Tu H84A complex with GppNHp Deposited 2017-07-06	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 59-meric	Chain Y 1–60（60 aa）	Not recorded	FME N-FORMYLMETHIONINE × 1 MG MAGNESIUM ION × 1904 K POTASSIUM ION × 5 PHE PHENYLALANINE × 1 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE；5 second blot time, no wait time	Resolution 3.00 Å
5WE4 70S ribosome-EF-Tu wt complex with GppNHp Deposited 2017-07-07	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 59-meric	Chain Y 1–60（60 aa）	Not recorded	MG MAGNESIUM ION × 1618 K POTASSIUM ION × 1 FME N-FORMYLMETHIONINE × 1 PHE PHENYLALANINE × 1 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE；5 second blot time, no wait time	Resolution 3.10 Å
5WE6 70S ribosome-EF-Tu H84A complex with GTP and cognate tRNA Deposited 2017-07-07	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 59-meric	Chain Y 1–60（60 aa）	Not recorded	FME N-FORMYLMETHIONINE × 1 MG MAGNESIUM ION × 662 K POTASSIUM ION × 8 PHE PHENYLALANINE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE；5 second blot time, no wait time	Resolution 3.40 Å
5WFK 70S ribosome-EF-Tu H84A complex with GTP and near-cognate tRNA (Complex C3) Deposited 2017-07-12	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 59-meric	Chain Y 1–60（60 aa）	Not recorded	FME N-FORMYLMETHIONINE × 1 MG MAGNESIUM ION × 1413 K POTASSIUM ION × 1 PHE PHENYLALANINE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE；5 second blot time, no wait time	Resolution 3.40 Å
6BU8 70S ribosome with S1 domains 1 and 2 (Class 1) Deposited 2017-12-08	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 53 PDB declaration: 60-meric	Chain 27 1–63（63 aa）	Not recorded	FME N-FORMYLMETHIONINE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE；2 uL of complex was applied to each grid. After a 10-second incubation, the grids were blotted for 2 to 4 seconds.	Resolution 3.50 Å
6BY1 E. coli pH03H9 complex Deposited 2017-12-19	Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 57-meric	Chain CY 1–63（63 aa）	Not recorded	MG MAGNESIUM ION × 225 ZN ZINC ION × 2	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；289 K；PEG 2000, PEP629, potassium thiocyanate, tris acetate	Resolution 3.94 Å R-free 0.269
6BY1 E. coli pH03H9 complex Deposited 2017-12-19	Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric	Chain DY 1–63（63 aa）	Not recorded	MG MAGNESIUM ION × 220 ZN ZINC ION × 2	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；289 K；PEG 2000, PEP629, potassium thiocyanate, tris acetate	Resolution 3.94 Å R-free 0.269
6ENF Cryo-EM structure of a polyproline-stalled ribosome in the absence of EF-P Deposited 2017-10-04	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 53-meric	Chain Y 1–63（63 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.20 Å
6ENJ Polyproline-stalled ribosome in the presence of A+P site tRNA and elongation-factor P (EF-P) Deposited 2017-10-05	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric	Chain Y 1–63（63 aa）	Not recorded	PRO PROLINE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.70 Å
6ENU Polyproline-stalled ribosome in the presence of elongation-factor P (EF-P) Deposited 2017-10-06	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 56-meric	Chain Y 1–63（63 aa）	Not recorded	PRO PROLINE × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.10 Å
6GBZ 50S ribosomal subunit assembly intermediate state 5 Deposited 2018-04-16	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 27 PDB declaration: 29-meric	Chain Y 1–63（63 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.80 Å
6GC0 50S ribosomal subunit assembly intermediate state 4 Deposited 2018-04-16	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 26 PDB declaration: 28-meric	Chain Y 1–63（63 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.80 Å
6GC4 50S ribosomal subunit assembly intermediate state 3 Deposited 2018-04-17	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 24 PDB declaration: 26-meric	Chain Y 1–63（63 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 4.30 Å
6GC6 50S ribosomal subunit assembly intermediate state 2 Deposited 2018-04-17	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 18 PDB declaration: nonadecameric	Chain Y 1–63（63 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 4.30 Å
6GC7 50S ribosomal subunit assembly intermediate state 1 Deposited 2018-04-17	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 16 PDB declaration: heptadecameric	Chain Y 1–63（63 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 4.30 Å
6GC8 50S ribosomal subunit assembly intermediate - 50S rec* Deposited 2018-04-17	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 27 PDB declaration: 29-meric	Chain Y 1–63（63 aa）	Not recorded	ZN ZINC ION × 1 MG MAGNESIUM ION × 5	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.80 Å
6GWT Cryo-EM structure of an E. coli 70S ribosome in complex with RF3-GDPCP, RF1(GAQ) and Pint-tRNA (State I) Deposited 2018-06-25	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 53 PDB declaration: 58-meric	Chain Y 1–63（63 aa）	Not recorded	GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4；Solutions were preapred fresh and filtered previous to usage. cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.80 Å
6GXM Cryo-EM structure of an E. coli 70S ribosome in complex with RF3-GDPCP, RF1(GAQ) and Pint-tRNA (State II) Deposited 2018-06-27	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 53 PDB declaration: 58-meric	Chain Y 1–63（63 aa）	Not recorded	GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4；Solutions were preapred fresh and filtered previous to usage. cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.80 Å
6GXN Cryo-EM structure of an E. coli 70S ribosome in complex with RF3-GDPCP, RF1(GAQ) and Pint-tRNA (State III) Deposited 2018-06-27	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 53 PDB declaration: 58-meric	Chain Y 1–63（63 aa）	Not recorded	GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4；Solutions were preapred fresh and filtered previous to usage. cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.90 Å
6GXO Cryo-EM structure of a rotated E. coli 70S ribosome in complex with RF3-GDPCP, RF1(GAQ) and P/E-tRNA (State IV) Deposited 2018-06-27	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 53 PDB declaration: 58-meric	Chain Y 1–63（63 aa）	Not recorded	GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4；Solutions were preapred fresh and filtered previous to usage. cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.90 Å
6GXP Cryo-EM structure of a rotated E. coli 70S ribosome in complex with RF3-GDPCP(RF3-only) Deposited 2018-06-27	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 55-meric	Chain Y 1–63（63 aa）	Not recorded	GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4；Solutions were preapred fresh and filtered previous to usage. cryo-EM vitrification conditions Cryogen ETHANE	Resolution 4.40 Å
6H4N Structure of a hibernating 100S ribosome reveals an inactive conformation of the ribosomal protein S1 - 70S Hibernating E. coli Ribosome Deposited 2018-07-22	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 57-meric	Chain Y 1–63（63 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5；B100S buffer (25 mM Hepes pH 7.5, 100 mM KOAc, 15 mM Mg(OAc)2, 1 mM dithiothreitol) cryo-EM vitrification conditions Cryogen ETHANE-PROPANE	Resolution 3.00 Å
6H58 Structure of a hibernating 100S ribosome reveals an inactive conformation of the ribosomal protein S1 - Full 100S Hibernating E. coli Ribosome Deposited 2018-07-24	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 104 PDB declaration: 112-meric	Chain Y 1–63（63 aa） Chain YY 1–63（63 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE-PROPANE	Resolution 7.90 Å
6HRM E. coli 70S d2d8 stapled ribosome Deposited 2018-09-27	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 53-meric	Chain Y 2–63（62 aa）	Not recorded	MG MAGNESIUM ION × 436 ZN ZINC ION × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.96 Å
6I0Y TnaC-stalled ribosome complex with the titin I27 domain folding close to the ribosomal exit tunnel Deposited 2018-10-26	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 33 PDB declaration: 36-meric	Chain Y 1–63（63 aa）	Not recorded	MG MAGNESIUM ION × 143 ZN ZINC ION × 1 TRP TRYPTOPHAN × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.20 Å
6I7V Ribosomal protein paralogs bL31 and bL36 Deposited 2018-11-19	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 99 PDB declaration: 105-meric	Chain CZ 2–63（62 aa） Chain DZ 2–63（62 aa）	Not recorded	MG MAGNESIUM ION × 453 PGE TRIETHYLENE GLYCOL × 8 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 13 PG4 TETRAETHYLENE GLYCOL × 5 SPD SPERMIDINE × 3 PUT 1,4-DIAMINOBUTANE × 7 1PE PENTAETHYLENE GLYCOL × 2 ACY ACETIC ACID × 3 PEG DI(HYDROXYETHYL)ETHER × 9 EDO 1,2-ETHANEDIOL × 12 GUN GUANINE × 1	X-RAY DIFFRACTION X-ray crystallization conditions MICROBATCH；pH 6.5；291 K；PEG8k, MPD, KSCN	Resolution 2.90 Å R-free 0.255
6OFX Non-rotated ribosome (Structure I) Deposited 2019-04-01	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 54-meric	Chain y 1–63（63 aa）	Not recorded	FME N-FORMYLMETHIONINE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.30 Å
6OG7 70S termination complex with RF2 bound to the UGA codon. Non-rotated ribosome with RF2 bound (Structure II) Deposited 2019-04-01	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric	Chain y 1–63（63 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.30 Å
6Q98 Structure of tmRNA SmpB bound in P site of E. coli 70S ribosome Deposited 2018-12-17	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 53 PDB declaration: 57-meric	Chain Y 1–63（63 aa）	Not recorded	MG MAGNESIUM ION × 429 ZN ZINC ION × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 4.30 Å
6Q9A Structure of tmRNA SmpB bound past E site of E. coli 70S ribosome Deposited 2018-12-17	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 53 PDB declaration: 58-meric	Chain Y 2–63（62 aa）	Not recorded	MG MAGNESIUM ION × 437 ZN ZINC ION × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.70 Å
6QUL Structure of a bacterial 50S ribosomal subunit in complex with the novel quinoxolidinone antibiotic cadazolid Deposited 2019-02-27	Different oligomeric state Different ligand/ion Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 28 PDB declaration: 31-meric	Chain Z 1–63（63 aa）	Not recorded	FME N-FORMYLMETHIONINE × 1 MG MAGNESIUM ION × 382 AMP ADENOSINE MONOPHOSPHATE × 1 JJH cadazolid × 1 ZN ZINC ION × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE-PROPANE	Resolution 3.00 Å
6S0K Ribosome nascent chain in complex with SecA Deposited 2019-06-17	Different oligomeric state Different ligand/ion Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 32 PDB declaration: 35-meric	Chain Z 1–63（63 aa）	Not recorded	MG MAGNESIUM ION × 68 ZN ZINC ION × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE-PROPANE	Resolution 3.10 Å
6SZS Release factor-dependent ribosome rescue by BrfA in the Gram-positive bacterium Bacillus subtilis Deposited 2019-10-02	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 56-meric	Chain Y 1–63（63 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.2 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.06 Å
6TBV Cryo-EM structure of an Escherichia coli ribosome-SpeFL complex stalled in response to L-ornithine (Replicate 2) Deposited 2019-11-04	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric	Chain L291 1–63（63 aa）	Not recorded	MG MAGNESIUM ION × 339 K POTASSIUM ION × 147 UNX UNKNOWN LIGAND × 1201 ZN ZINC ION × 3 ORN L-ornithine × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.70 Å
6TC3 Cryo-EM structure of an Escherichia coli ribosome-SpeFL complex stalled in response to L-ornithine (Replicate 1) Deposited 2019-11-05	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric	Chain L291 1–63（63 aa）	Not recorded	MG MAGNESIUM ION × 339 K POTASSIUM ION × 147 UNX UNKNOWN LIGAND × 1201 ZN ZINC ION × 3 ORN L-ornithine × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.70 Å
6VWL 70S ribosome bound to HIV frameshifting stem-loop (FSS) and P/E tRNA (rotated conformation) Deposited 2020-02-20	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 53-meric	Chain W 1–63（63 aa）	Not recorded	MG MAGNESIUM ION × 34	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.10 Å
6VWM 70S ribosome bound to HIV frameshifting stem-loop (FSS) and P-site tRNA (non-rotated conformation, Structure I) Deposited 2020-02-20	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 53-meric	Chain W 1–63（63 aa）	Not recorded	MG MAGNESIUM ION × 53	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.40 Å
6VWN 70S ribosome bound to HIV frameshifting stem-loop (FSS) and P-site tRNA (non-rotated conformation, Structure II) Deposited 2020-02-20	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 53-meric	Chain W 1–63（63 aa）	Not recorded	MG MAGNESIUM ION × 55	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.40 Å
6WD6 Cryo-EM of elongating ribosome with EF-Tu*GTP elucidates tRNA proofreading (Cognate Structure II-C2) Deposited 2020-03-31	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 59-meric	Chain y 1–63（63 aa）	Not recorded	FME N-FORMYLMETHIONINE × 1 PHE PHENYLALANINE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.70 Å
6WDB Cryo-EM of elongating ribosome with EF-Tu*GTP elucidates tRNA proofreading (Cognate Structure IV-A) Deposited 2020-03-31	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 58-meric	Chain y 1–63（63 aa）	Not recorded	FME N-FORMYLMETHIONINE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 4.00 Å
6WDC Cryo-EM of elongating ribosome with EF-Tu*GTP elucidates tRNA proofreading (Cognate Structure IV-B) Deposited 2020-03-31	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 58-meric	Chain y 1–63（63 aa）	Not recorded	FME N-FORMYLMETHIONINE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 4.20 Å
6WDD Cryo-EM of elongating ribosome with EF-Tu*GTP elucidates tRNA proofreading (Cognate Structure V-A) Deposited 2020-03-31	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 58-meric	Chain y 1–63（63 aa）	Not recorded	FME N-FORMYLMETHIONINE × 1 PHE PHENYLALANINE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.20 Å
6WDE Cryo-EM of elongating ribosome with EF-Tu*GTP elucidates tRNA proofreading (Cognate Structure V-B) Deposited 2020-03-31	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 58-meric	Chain y 1–63（63 aa）	Not recorded	PHE PHENYLALANINE × 1 MET METHIONINE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.00 Å
6WDF Cryo-EM of elongating ribosome with EF-Tu*GTP elucidates tRNA proofreading (Cognate Structure VI-A) Deposited 2020-03-31	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 57-meric	Chain y 1–63（63 aa）	Not recorded	PHE PHENYLALANINE × 1 FME N-FORMYLMETHIONINE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.30 Å
6WDG Cryo-EM of elongating ribosome with EF-Tu*GTP elucidates tRNA proofreading (Cognate Structure VI-B) Deposited 2020-03-31	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 57-meric	Chain y 1–63（63 aa）	Not recorded	PHE PHENYLALANINE × 1 FME N-FORMYLMETHIONINE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.30 Å
6WDH Cryo-EM of elongating ribosome with EF-Tu*GTP elucidates tRNA proofreading (Non-cognate Structure IV-B1) Deposited 2020-03-31	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 58-meric	Chain y 1–63（63 aa）	Not recorded	FME N-FORMYLMETHIONINE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 4.30 Å
6WDI Cryo-EM of elongating ribosome with EF-Tu*GTP elucidates tRNA proofreading (Non-cognate Structure IV-B2) Deposited 2020-03-31	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 58-meric	Chain y 1–63（63 aa）	Not recorded	FME N-FORMYLMETHIONINE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 4.00 Å
6WDJ Cryo-EM of elongating ribosome with EF-Tu*GTP elucidates tRNA proofreading (Non-cognate Structure V-A1) Deposited 2020-03-31	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 58-meric	Chain y 1–63（63 aa）	Not recorded	FME N-FORMYLMETHIONINE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.70 Å
6WDK Cryo-EM of elongating ribosome with EF-Tu*GTP elucidates tRNA proofreading (Non-cognate Structure V-A2) Deposited 2020-03-31	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 58-meric	Chain y 1–63（63 aa）	Not recorded	FME N-FORMYLMETHIONINE × 1 PHE PHENYLALANINE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.60 Å
6WDL Cryo-EM of elongating ribosome with EF-Tu*GTP elucidates tRNA proofreading (Non-cognate Structure V-B1) Deposited 2020-03-31	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 58-meric	Chain y 1–63（63 aa）	Not recorded	FME N-FORMYLMETHIONINE × 1 PHE PHENYLALANINE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.70 Å
6WDM Cryo-EM of elongating ribosome with EF-Tu*GTP elucidates tRNA proofreading (Non-cognate Structure V-B2) Deposited 2020-03-31	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 58-meric	Chain y 1–63（63 aa）	Not recorded	PHE PHENYLALANINE × 1 FME N-FORMYLMETHIONINE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.60 Å
6WNT 50S ribosomal subunit without free 5S rRNA and perturbed PTC Deposited 2020-04-23	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 30 PDB declaration: 31-meric	Chain y 1–63（63 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.10 Å
6WNV 70S ribosome without free 5S rRNA and with a perturbed PTC Deposited 2020-04-23	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 52-meric	Chain y 1–63（63 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.50 Å
6WNW Active 70S ribosome without free 5S rRNA and bound with A- and P- tRNA Deposited 2020-04-23	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 57-meric	Chain y 1–63（63 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.20 Å
6XZ7 E. coli 50S ribosomal subunit in complex with dirithromycin, fMet-Phe-tRNA(Phe) and deacylated tRNA(iMet). Deposited 2020-02-03	Different construct Different oligomeric state Different ligand/ion Different experimental conditions	Assembly 1 Protein–RNA Heteromer；Protein × 29 PDB declaration: 33-meric	Chain Y 2–63（62 aa）	Not recorded	MG MAGNESIUM ION × 178 DI0 Dirithromycin × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.10 Å
6XZA E. coli 70S ribosome in complex with dirithromycin, and deacylated tRNA(iMet) (focused classification). Deposited 2020-02-03	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric	Chain Y2 2–63（62 aa）	Not recorded	DI0 Dirithromycin × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.66 Å
6XZB E. coli 70S ribosome in complex with dirithromycin, fMet-Phe-tRNA(Phe) and deacylated tRNA(iMet) (focused classification). Deposited 2020-02-03	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 54-meric	Chain Y2 2–63（62 aa）	Not recorded	DI0 Dirithromycin × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.54 Å
6Y69 Cryo-EM structure of an Escherichia coli 70S ribosome in complex with antibiotic TetracenomycinX Deposited 2020-02-26	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 54-meric	Chain Y 1–63（63 aa）	Not recorded	OCW Tetracenomycin X × 1 MG MAGNESIUM ION × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.86 Å
6YSR Structure of the P+9 stalled ribosome complex Deposited 2020-04-23	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 57-meric	Chain Y 1–63（63 aa）	Not recorded	MG MAGNESIUM ION × 332 ZN ZINC ION × 2 CL CHLORIDE ION × 1 NA SODIUM ION × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4；50 mM HEPES, 30 mM KCl, 7 mM MgCl2 cryo-EM vitrification conditions Cryogen ETHANE；Manual blotting & plunge-freezing	Resolution 3.10 Å
6YSS Structure of the P+9 ArfB-ribosome complex in the post-hydrolysis state Deposited 2020-04-23	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 53 PDB declaration: 58-meric	Chain Y 1–63（63 aa）	Not recorded	MG MAGNESIUM ION × 387 ZN ZINC ION × 2 NA SODIUM ION × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4；50 mM HEPES, 30 mM KCl, 7 mM MgCl2 cryo-EM vitrification conditions Cryogen ETHANE；Manual blotting & plunge-freezing	Resolution 2.60 Å
6YST Structure of the P+9 ArfB-ribosome complex with P/E hybrid tRNA in the post-hydrolysis state Deposited 2020-04-23	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 53 PDB declaration: 58-meric	Chain Y 1–63（63 aa）	Not recorded	MG MAGNESIUM ION × 253 ZN ZINC ION × 2 NA SODIUM ION × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4；50 mM HEPES, 30 mM KCl, 7 mM MgCl2 cryo-EM vitrification conditions Cryogen ETHANE；Manual blotting & plunge-freezing	Resolution 3.20 Å
6YSU Structure of the P+0 ArfB-ribosome complex in the post-hydrolysis state Deposited 2020-04-23	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 57-meric	Chain Y 1–63（63 aa）	Not recorded	MG MAGNESIUM ION × 356 ZN ZINC ION × 1 NA SODIUM ION × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4；50 mM HEPES, 30 mM KCl, 7 mM MgCl2, 0.1 % w/v DDM cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.70 Å
6ZTJ E. coli 70S-RNAP expressome complex in NusG-coupled state (38 nt intervening mRNA) Deposited 2020-07-20	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Other combination Heteromer；Protein × 58 PDB declaration: 66-meric	Chain BZ 1–63（63 aa）	Not recorded	MG MAGNESIUM ION × 479 PHE PHENYLALANINE × 1 ZN ZINC ION × 4	ELECTRON MICROSCOPY cryo-EM buffer pH 8 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.40 Å
6ZTL E. coli 70S-RNAP expressome complex in collided state bound to NusG Deposited 2020-07-20	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Other combination Heteromer；Protein × 54 PDB declaration: 62-meric	Chain BZ 1–63（63 aa）	Not recorded	MG MAGNESIUM ION × 441 PHE PHENYLALANINE × 1 ZN ZINC ION × 3	ELECTRON MICROSCOPY cryo-EM buffer pH 8 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.50 Å
6ZTM E. coli 70S-RNAP expressome complex in collided state without NusG Deposited 2020-07-20	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Other combination Heteromer；Protein × 52 PDB declaration: 60-meric	Chain BZ 1–63（63 aa）	Not recorded	MG MAGNESIUM ION × 421 PHE PHENYLALANINE × 1 ZN ZINC ION × 3	ELECTRON MICROSCOPY cryo-EM buffer pH 8 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.30 Å
6ZTN E. coli 70S-RNAP expressome complex in NusG-coupled state (42 nt intervening mRNA) Deposited 2020-07-20	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Other combination Heteromer；Protein × 54 PDB declaration: 63-meric	Chain BZ 1–63（63 aa）	Not recorded	MG MAGNESIUM ION × 487 PHE PHENYLALANINE × 1 ZN ZINC ION × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 8 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.90 Å
6ZTO E. coli 70S-RNAP expressome complex in uncoupled state 1 Deposited 2020-07-20	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Other combination Heteromer；Protein × 54 PDB declaration: 62-meric	Chain BZ 1–63（63 aa）	Not recorded	MG MAGNESIUM ION × 487 PHE PHENYLALANINE × 1 ZN ZINC ION × 4	ELECTRON MICROSCOPY cryo-EM buffer pH 8 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.00 Å
6ZTP E. coli 70S-RNAP expressome complex in uncoupled state 6 Deposited 2020-07-20	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Other combination Heteromer；Protein × 54 PDB declaration: 62-meric	Chain BZ 1–63（63 aa）	Not recorded	MG MAGNESIUM ION × 487 PHE PHENYLALANINE × 1 ZN ZINC ION × 4	ELECTRON MICROSCOPY cryo-EM buffer pH 8 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.00 Å
6ZU1 E. coli 70S-RNAP expressome complex in uncoupled state 2 Deposited 2020-07-21	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Other combination Heteromer；Protein × 54 PDB declaration: 62-meric	Chain BZ 1–63（63 aa）	Not recorded	MG MAGNESIUM ION × 487 PHE PHENYLALANINE × 1 ZN ZINC ION × 4	ELECTRON MICROSCOPY cryo-EM buffer pH 8 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.00 Å
7ABZ Structure of pre-accomodated trans-translation complex on E. coli stalled ribosome. Deposited 2020-09-09	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 58-meric	Chain Y 2–63（62 aa）	Not recorded	MG MAGNESIUM ION × 192 KIR KIRROMYCIN × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ZN ZINC ION × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE；blot for 3.5 seconds before plunging	Resolution 3.21 Å
7AC7 Structure of accomodated trans-translation complex on E. Coli stalled ribosome. Deposited 2020-09-10	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 58-meric	Chain Y 3–60（58 aa）	Not recorded	MG MAGNESIUM ION × 441 ZN ZINC ION × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE；blot for 3.5 seconds before plunging	Resolution 3.08 Å
7ACJ Structure of translocated trans-translation complex on E. coli stalled ribosome. Deposited 2020-09-11	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 55-meric	Chain Y 2–63（62 aa）	Not recorded	MG MAGNESIUM ION × 308 ZN ZINC ION × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE；blot for 3.5 seconds before plunging	Resolution 3.20 Å
7ACR Structure of post-translocated trans-translation complex on E. coli stalled ribosome. Deposited 2020-09-11	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 55-meric	Chain Y 2–63（62 aa）	Not recorded	MG MAGNESIUM ION × 212 ZN ZINC ION × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE；blot for 3.5 seconds before plunging	Resolution 3.44 Å
7B5K E. coli 70S containing suppressor tRNA in the A-site stabilized by a Negamycin analogue and P-site tRNA-nascent chain. Deposited 2020-12-03	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 57-meric	Chain Y 2–63（62 aa）	Not recorded	MG MAGNESIUM ION × 359 ERY ERYTHROMYCIN A × 1 SY5 2-[[[(3~{R},5~{R})-3-azanyl-6-(3-azanylpropylamino)-5-oxidanyl-hexanoyl]amino]-methyl-amino]ethanoic acid × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE-PROPANE	Resolution 2.90 Å
7BL2 pre-50S-ObgE particle state 1 Deposited 2021-01-18	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 29 PDB declaration: 31-meric	Chain Y 1–63（63 aa）	Not recorded	MG MAGNESIUM ION × 1 ZN ZINC ION × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.70 Å
7BL3 pre-50S-ObgE particle state 2 Deposited 2021-01-18	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 29 PDB declaration: 31-meric	Chain Y 1–63（63 aa）	Not recorded	ZN ZINC ION × 1 MG MAGNESIUM ION × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.50 Å
7BL4 in vitro reconstituted 50S-ObgE-GMPPNP-RsfS particle Deposited 2021-01-18	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 31 PDB declaration: 33-meric	Chain Y 1–63（63 aa）	Not recorded	ZN ZINC ION × 2 MG MAGNESIUM ION × 6 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.40 Å
7BL5 pre-50S-ObgE particle Deposited 2021-01-18	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 33 PDB declaration: 35-meric	Chain Y 1–63（63 aa）	Not recorded	GDP GUANOSINE-5'-DIPHOSPHATE × 1 MG MAGNESIUM ION × 2 ZN ZINC ION × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.30 Å
7BL6 50S-ObgE-GMPPNP particle Deposited 2021-01-18	Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 30 PDB declaration: 32-meric	Chain Y 1–63（63 aa）	Not recorded	ZN ZINC ION × 2 MG MAGNESIUM ION × 2 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 4.00 Å
7BV8 Mature 50S ribosomal subunit from RrmJ knock out E.coli strain Deposited 2020-04-09	Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 30 PDB declaration: 32-meric	Chain Z 1–63（63 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5；Solutions were made fresh form concentrated to avoid microbial contamination. cryo-EM vitrification conditions Cryogen ETHANE；blot for 2 seconds before plunging	Resolution 3.14 Å
7D6Z Molecular model of the cryo-EM structure of 70S ribosome in complex with peptide deformylase and trigger factor Deposited 2020-10-02	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 58-meric	Chain Y 1–63（63 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.40 Å
7D80 Molecular model of the cryo-EM structure of 70S ribosome in complex with peptide deformylase, trigger factor, and methionine aminopeptidase Deposited 2020-10-06	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 57-meric	Chain w 1–63（63 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 4.10 Å
7JSS ArfB Rescue of a 70S Ribosome stalled on truncated mRNA with a partial A-site codon (+2-II) Deposited 2020-08-15	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 54-meric	Chain y 1–63（63 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.70 Å
7JSW ArfB Rescue of a 70S Ribosome stalled on truncated mRNA with a partial A-site codon (+2-III) Deposited 2020-08-16	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 54-meric	Chain y 1–63（63 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.80 Å
7JSZ ArfB Rescue of a 70S Ribosome stalled on truncated mRNA with a partial A-site codon (+2-IV) Deposited 2020-08-16	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 57-meric	Chain y 1–63（63 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.70 Å
7JT1 70S ribosome stalled on long mRNA with ArfB-1 and ArfB-2 bound (+9-III) Deposited 2020-08-16	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric	Chain y 1–63（63 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.30 Å
7JT2 70S ribosome stalled on long mRNA with ArfB bound in the A site Deposited 2020-08-16	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric	Chain y 1–63（63 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.50 Å
7JT3 Rotated 70S ribosome stalled on long mRNA with ArfB-1 and ArfB-2 bound in the A site (+9-IV) Deposited 2020-08-17	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 53 PDB declaration: 58-meric	Chain y 1–63（63 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.70 Å
7K50 Pre-translocation non-frameshifting(CCA-A) complex (Structure I) Deposited 2020-09-16	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 59-meric	Chain y 1–63（63 aa）	Not recorded	FME N-FORMYLMETHIONINE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE；Blotting force 9, blotted for 4 seconds	Resolution 3.40 Å
7K51 Mid-translocated non-frameshifting(CCA-A) complex with EF-G and GDPCP (Structure II) Deposited 2020-09-16	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 53 PDB declaration: 59-meric	Chain y 1–63（63 aa）	Not recorded	FME N-FORMYLMETHIONINE × 1 PRO PROLINE × 1 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE；Blotting force 9, blotted for 4 seconds	Resolution 3.50 Å
7K52 Near post-translocated non-frameshifting(CCA-A) complex with EF-G and GDPCP (Structure III) Deposited 2020-09-16	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 53 PDB declaration: 59-meric	Chain y 1–63（63 aa）	Not recorded	FME N-FORMYLMETHIONINE × 1 PRO PROLINE × 1 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE；blotting force 9, blotted for 4 seconds	Resolution 3.40 Å
7K53 Pre-translocation +1-frameshifting(CCC-A) complex (Structure I-FS) Deposited 2020-09-16	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 59-meric	Chain y 1–63（63 aa）	Not recorded	FME N-FORMYLMETHIONINE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE；Blotting force 9, blotted for 4 seconds	Resolution 3.20 Å
7K54 Mid-translocated +1-frameshifting(CCC-A) complex with EF-G and GDPCP (Structure II-FS) Deposited 2020-09-16	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 53 PDB declaration: 59-meric	Chain y 1–63（63 aa）	Not recorded	FME N-FORMYLMETHIONINE × 1 PRO PROLINE × 1 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE；Blotting force 9, blotted for 4 seconds	Resolution 3.20 Å
7K55 Near post-translocated +1-frameshifting(CCC-A) complex with EF-G and GDPCP (Structure III-FS) Deposited 2020-09-16	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 53 PDB declaration: 59-meric	Chain y 1–63（63 aa）	Not recorded	FME N-FORMYLMETHIONINE × 1 PRO PROLINE × 1 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE；blotting force 9, blotted for 4 seconds	Resolution 3.30 Å
7LV0 Pre-translocation rotated ribosome +1-frameshifting(CCC-A) complex (Structure Irot-FS) Deposited 2021-02-23	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 58-meric	Chain y 1–63（63 aa）	Not recorded	FME N-FORMYLMETHIONINE × 1 PRO PROLINE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE；Blotting force 9, blotted for 4 seconds	Resolution 3.20 Å
7N1P Elongating 70S ribosome complex in a classical pre-translocation (PRE-C) conformation Deposited 2021-05-28	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 58-meric	Chain Lc 1–63（63 aa）	Not recorded	PUT 1,4-DIAMINOBUTANE × 18 MG MAGNESIUM ION × 405 ZN ZINC ION × 3 ATP ADENOSINE-5'-TRIPHOSPHATE × 3 SPD SPERMIDINE × 5	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.33 Å
7N2C Elongating 70S ribosome complex in a fusidic acid-stalled intermediate state of translocation bound to EF-G(GDP) (INT2) Deposited 2021-05-28	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 55 PDB declaration: 61-meric	Chain Lc 1–63（63 aa）	Not recorded	PUT 1,4-DIAMINOBUTANE × 21 MG MAGNESIUM ION × 197 ZN ZINC ION × 3 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 SPD SPERMIDINE × 3 FUA FUSIDIC ACID × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.72 Å
7N2U Elongating 70S ribosome complex in a hybrid-H1 pre-translocation (PRE-H1) conformation Deposited 2021-05-29	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric	Chain Lc 1–63（63 aa）	Not recorded	PUT 1,4-DIAMINOBUTANE × 16 MG MAGNESIUM ION × 368 ZN ZINC ION × 3 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 SPD SPERMIDINE × 5	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.53 Å
7N2V Elongating 70S ribosome complex in a spectinomycin-stalled intermediate state of translocation bound to EF-G in an active, GTP conformation (INT1) Deposited 2021-05-29	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 53 PDB declaration: 59-meric	Chain Lc 1–63（63 aa）	Not recorded	SCM SPECTINOMYCIN × 3 PUT 1,4-DIAMINOBUTANE × 17 MG MAGNESIUM ION × 344 ZN ZINC ION × 3 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 SPD SPERMIDINE × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.54 Å
7N30 Elongating 70S ribosome complex in a hybrid-H2* pre-translocation (PRE-H2*) conformation Deposited 2021-05-30	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric	Chain Lc 1–63（63 aa）	Not recorded	PUT 1,4-DIAMINOBUTANE × 11 MG MAGNESIUM ION × 296 ZN ZINC ION × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 SPD SPERMIDINE × 4	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.66 Å
7N31 Elongating 70S ribosome complex in a post-translocation (POST) conformation Deposited 2021-05-31	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric	Chain Lc 1–63（63 aa）	Not recorded	MG MAGNESIUM ION × 209 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 PUT 1,4-DIAMINOBUTANE × 9 SPD SPERMIDINE × 4 ZN ZINC ION × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.69 Å
7NBU Structure of the HigB1 toxin mutant K95A from Mycobacterium tuberculosis (Rv1955) and its target, the cspA mRNA, on the E. coli Ribosome. Deposited 2021-01-27	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Insufficient information Heteromer；Protein × 50 PDB declaration: 56-meric	Chain x 2–63（62 aa）	Not recorded	MG MAGNESIUM ION × 313 FME N-FORMYLMETHIONINE × 1 ZN ZINC ION × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE；blot for 2s before plunging	Resolution 3.11 Å
7NSO Structure of ErmDL-Erythromycin-stalled 70S E. coli ribosomal complex with P-tRNA Deposited 2021-03-08	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric	Chain Y 1–63（63 aa）	Not recorded	ERY ERYTHROMYCIN A × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE-PROPANE	Resolution 2.90 Å
7NSP Structure of ErmDL-Erythromycin-stalled 70S E. coli ribosomal complex with A and P-tRNA Deposited 2021-03-08	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric	Chain Y 1–63（63 aa）	Not recorded	ERY ERYTHROMYCIN A × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE-PROPANE	Resolution 3.50 Å
7NSQ Structure of ErmDL-Telithromycin-stalled 70S E. coli ribosomal complex with A and P-tRNA Deposited 2021-03-08	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric	Chain Y 1–63（63 aa）	Not recorded	TEL TELITHROMYCIN × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE-PROPANE	Resolution 3.10 Å
7NWT Initiated 70S ribosome in complex with 2A protein from encephalomyocarditis virus (EMCV) Deposited 2021-03-17	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 54 PDB declaration: 59-meric	Chain Y 1–63（63 aa）	Not recorded	MG MAGNESIUM ION × 437 FME N-FORMYLMETHIONINE × 1 ZN ZINC ION × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5；Initiated 70S ribosomes in 50 mM Tris-HCl pH 7.5, 70 mM NH4Cl, 30 mM KCl, 7 mM MgCl2 were diluted tenfold into 20 mM HEPES pH 7.5, 100 mM potassium acetate, 1.5 mM MgCl2, 2.0 mM DTT. 2A protein was dialysed (3K MWCO, 277K, 16 h) into the same buffer. Crosslinking reactions of 50 microliters comprising 75 nM ribosomes, 3.0 micromolar 2A and 2.0 mM bis(sulfosuccinimidyl)suberate (BS3) were performed on ice (30 min) immediately prior to grid preparation. cryo-EM vitrification conditions Cryogen ETHANE；Quantifoil R 2/2 400-mesh copper supports were coated with an additional ~ 60 angstrom layer of amorphous, evaporated carbon by flotation and thoroughly dried before use. Grids were made hydrophilic by glow-discharge in air for 30 s. Three microliters of crosslinking reaction was applied to grids which were then blotted for 4.5 s and vitrified by plunging into liquid ethane using a Vitrobot MK IV (FEI) at 277K, 100% relative humidity.	Resolution 2.66 Å
7O19 Cryo-EM structure of an Escherichia coli TnaC-ribosome complex stalled in response to L-tryptophan Deposited 2021-03-29	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric	Chain BY 1–63（63 aa）	Not recorded	MG MAGNESIUM ION × 170 ZN ZINC ION × 3 TRP TRYPTOPHAN × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5；All buffers used during the preparation contained 2 mM L-tryptophan. cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.90 Å
7O1A Cryo-EM structure of an Escherichia coli TnaC(R23F)-ribosome complex stalled in response to L-tryptophan Deposited 2021-03-29	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric	Chain BY 1–63（63 aa）	Not recorded	MG MAGNESIUM ION × 326 K POTASSIUM ION × 147 ZN ZINC ION × 3 TRP TRYPTOPHAN × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5；All buffers used during the preparation contained 2 mM L-tryptophan. cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.40 Å
7O1C Cryo-EM structure of an Escherichia coli TnaC(R23F)-ribosome-RF2 complex stalled in response to L-tryptophan Deposited 2021-03-29	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 56-meric	Chain BY 1–63（63 aa）	Not recorded	MG MAGNESIUM ION × 339 K POTASSIUM ION × 147 ZN ZINC ION × 3 TRP TRYPTOPHAN × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5；All buffers used during the preparation contained 2 mM L-tryptophan. cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.60 Å
7ODE E. coli 50S ribosome LiCl core particle Deposited 2021-04-29	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 15 PDB declaration: hexadecameric	Chain g 1–63（63 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE；continuous carbon, 3 microL of sample, incubate for 30 seconds, blot for 3.5 seconds	Resolution 2.84 Å
7OIZ Cryo-EM structure of 70S ribosome stalled with TnaC peptide Deposited 2021-05-13	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric	Chain x 1–63（63 aa）	Not recorded	PAR PAROMOMYCIN × 1 MG MAGNESIUM ION × 308 TRP TRYPTOPHAN × 1 ZN ZINC ION × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.90 Å
7OJ0 Cryo-EM structure of 70S ribosome stalled with TnaC peptide and RF2 Deposited 2021-05-13	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 54 PDB declaration: 59-meric	Chain x 1–63（63 aa）	Not recorded	MG MAGNESIUM ION × 308 TRP TRYPTOPHAN × 1 ZN ZINC ION × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.50 Å
7P3K Cryo-EM structure of 70S ribosome stalled with TnaC peptide (control) Deposited 2021-07-08	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 54-meric	Chain x 1–63（63 aa）	Not recorded	MG MAGNESIUM ION × 308 CLM CHLORAMPHENICOL × 1 ZN ZINC ION × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.90 Å
7PJS Structure of the 70S ribosome with tRNAs in the classical pre-translocation state and apramycin (C) Deposited 2021-08-24	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 58-meric	Chain Y 1–63（63 aa）	Not recorded	MG MAGNESIUM ION × 363 ZN ZINC ION × 2 NA SODIUM ION × 2 AM2 APRAMYCIN × 4	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5；50 mM HEPES, 70 mM NH4Cl, 30 mM KCl, 3.5 mM MgCl2, 0.6 mM spermine, 0.4 mM spermidine cryo-EM vitrification conditions Cryogen ETHANE；Manual blotting & plunge-freezing	Resolution 2.35 Å
7PJT Structure of the 70S ribosome with tRNAs in hybrid state 1 (H1) Deposited 2021-08-24	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 58-meric	Chain Y 1–63（63 aa）	Not recorded	ZN ZINC ION × 2 AM2 APRAMYCIN × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE；Manual blotting & plunge-freezing	Resolution 6.00 Å
7PJV Structure of the 70S-EF-G-GDP-Pi ribosome complex with tRNAs in hybrid state 1 (H1-EF-G-GDP-Pi) Deposited 2021-08-24	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 53 PDB declaration: 59-meric	Chain Y 1–63（63 aa）	Not recorded	MG MAGNESIUM ION × 369 ZN ZINC ION × 2 NA SODIUM ION × 2 AM2 APRAMYCIN × 3 GDP GUANOSINE-5'-DIPHOSPHATE × 1 PO4 PHOSPHATE ION × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5；50 mM HEPES, 70 mM NH4Cl, 30 mM KCl, 3.5 mM MgCl2, 0.6 mM spermine, 0.4 mM spermidine cryo-EM vitrification conditions Cryogen ETHANE；Manual blotting & plunge-freezing	Resolution 3.10 Å
7PJW Structure of the 70S-EF-G-GDP-Pi ribosome complex with tRNAs in hybrid state 2 (H2-EF-G-GDP-Pi) Deposited 2021-08-24	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 53 PDB declaration: 59-meric	Chain Y 1–63（63 aa）	Not recorded	AM2 APRAMYCIN × 1 PO4 PHOSPHATE ION × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5；50 mM HEPES, 70 mM NH4Cl, 30 mM KCl, 3.5 mM MgCl2, 0.6 mM spermine, 0.4 mM spermidine cryo-EM vitrification conditions Cryogen ETHANE；Manual blotting & plunge-freezing	Resolution 4.00 Å
7PJX Structure of the 70S-EF-G-GDP ribosome complex with tRNAs in hybrid state 1 (H1-EF-G-GDP) Deposited 2021-08-24	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 53 PDB declaration: 59-meric	Chain Y 1–63（63 aa）	Not recorded	AM2 APRAMYCIN × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5；50 mM HEPES, 70 mM NH4Cl, 30 mM KCl, 3.5 mM MgCl2, 0.6 mM spermine, 0.4 mM spermidine cryo-EM vitrification conditions Cryogen ETHANE；Manual blotting & plunge-freezing	Resolution 6.50 Å
7PJY Structure of the 70S-EF-G-GDP ribosome complex with tRNAs in chimeric state 1 (CHI1-EF-G-GDP) Deposited 2021-08-24	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 53 PDB declaration: 59-meric	Chain Y 1–63（63 aa）	Not recorded	MG MAGNESIUM ION × 318 ZN ZINC ION × 2 NA SODIUM ION × 1 AM2 APRAMYCIN × 4 GDP GUANOSINE-5'-DIPHOSPHATE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5；50 mM HEPES, 70 mM NH4Cl, 30 mM KCl, 3.5 mM MgCl2, 0.6 mM spermine, 0.4 mM spermidine cryo-EM vitrification conditions Cryogen ETHANE；Manual blotting & plunge-freezing	Resolution 3.10 Å
7PJZ Structure of the 70S-EF-G-GDP ribosome complex with tRNAs in chimeric state 2 (CHI2-EF-G-GDP) Deposited 2021-08-24	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 53 PDB declaration: 59-meric	Chain Y 1–63（63 aa）	Not recorded	ZN ZINC ION × 1 AM2 APRAMYCIN × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5；50 mM HEPES, 70 mM NH4Cl, 30 mM KCl, 3.5 mM MgCl2, 0.6 mM spermine, 0.4 mM spermidine cryo-EM vitrification conditions Cryogen ETHANE；Manual blotting & plunge-freezing	Resolution 6.00 Å
7Q4K Erythromycin-stalled Escherichia coli 70S ribosome with streptococcal MsrDL nascent chain Deposited 2021-10-31	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric	Chain BY 1–63（63 aa）	Not recorded	MG MAGNESIUM ION × 172 ERY ERYTHROMYCIN A × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.00 Å
7QG8 Structure of the collided E. coli disome - VemP-stalled 70S ribosome Deposited 2021-12-07	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 57-meric	Chain l 1–63（63 aa）	Not recorded	MG MAGNESIUM ION × 2 K POTASSIUM ION × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.97 Å
7QGH Structure of the E. coli disome - collided 70S ribosome Deposited 2021-12-08	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 58-meric	Chain l 1–63（63 aa）	Not recorded	MG MAGNESIUM ION × 2 K POTASSIUM ION × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 4.48 Å
7QGN Structure of the SmrB-bound E. coli disome - stalled 70S ribosome Deposited 2021-12-09	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 53 PDB declaration: 58-meric	Chain l 1–63（63 aa）	Not recorded	MG MAGNESIUM ION × 2 K POTASSIUM ION × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.37 Å
7QGR Structure of the SmrB-bound E. coli disome - collided 70S ribosome Deposited 2021-12-09	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 53 PDB declaration: 59-meric	Chain l 1–63（63 aa）	Not recorded	MG MAGNESIUM ION × 2 K POTASSIUM ION × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 5.70 Å
7QQ3 Cryo-EM structure of the E.coli 50S ribosomal subunit in complex with the antibiotic Myxovalargin A. Deposited 2022-01-06	Different oligomeric state Different ligand/ion Different experimental conditions	Assembly 1 Protein–RNA Heteromer；Protein × 27 PDB declaration: 29-meric	Chain g 1–63（63 aa）	Not recorded	MG MAGNESIUM ION × 145 ZN ZINC ION × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5；Solutions were made fresh, pH was adjusted with potassium hydroxide at 4 degrees celcius and sterile filtered. cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；2 seconds blotting before plunging.	Resolution 2.10 Å
7SS9 Late translocation intermediate with EF-G partially dissociated (Structure V) Deposited 2021-11-10	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 57-meric	Chain y 1–63（63 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.90 Å
7SSD Mid translocation intermediate with EF-G bound with GDP (Structure IV) Deposited 2021-11-10	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 58-meric	Chain y 1–63（63 aa）	Not recorded	GDP GUANOSINE-5'-DIPHOSPHATE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.30 Å
7SSL Pre translocation intermediate with EF-G bound to GDP and Pi (Structure III) Deposited 2021-11-11	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 58-meric	Chain y 1–63（63 aa）	Not recorded	GDP GUANOSINE-5'-DIPHOSPHATE × 1 PO4 PHOSPHATE ION × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.80 Å
7SSN Pre translocation 70S ribosome with A/P* and P/E tRNA (Structure II-B) Deposited 2021-11-11	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 54-meric	Chain y 1–63（63 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.20 Å
7SSO Pre translocation 70S ribosome with A/A and P/E tRNA (Structure II-A) Deposited 2021-11-11	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 58-meric	Chain y 1–63（63 aa）	Not recorded	FME N-FORMYLMETHIONINE × 1 PRO PROLINE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.20 Å
7SSW Late translocation intermediate with EF-G dissociated (Structure VI) Deposited 2021-11-11	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric	Chain y 1–63（63 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.80 Å
7ST2 Post translocation, non-rotated 70S ribosome with EF-G dissociated (Structure VII) Deposited 2021-11-11	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 58-meric	Chain y 1–63（63 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.90 Å
7ST6 Pre translocation, non-rotated 70S ribosome (Structure I) Deposited 2021-11-12	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 57-meric	Chain y 1–63（63 aa）	Not recorded	PRO PROLINE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.00 Å
7ST7 Pre translocation intermediate stalled with viomycin and bound with EF-G in a GDP and Pi state (Structure III-vio) Deposited 2021-11-12	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 53 PDB declaration: 59-meric	Chain y 1–63（63 aa）	Not recorded	GDP GUANOSINE-5'-DIPHOSPHATE × 1 PO4 PHOSPHATE ION × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.20 Å
7Y7C Structure of the Bacterial Ribosome with human tRNA Asp(G34) and mRNA(GAU) Deposited 2022-06-22	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric	Chain x 1–63（63 aa）	Not recorded	MG MAGNESIUM ION × 283	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6；The Buffer pH was adjusted to 7.6 using KOH. cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.51 Å
7Y7D Structure of the Bacterial Ribosome with human tRNA Asp(Q34) and mRNA(GAU) Deposited 2022-06-22	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric	Chain x 1–63（63 aa）	Not recorded	MG MAGNESIUM ION × 258	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6；The Buffer pH was adjusted to 7.6 using KOH. cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.58 Å
7Y7E Structure of the Bacterial Ribosome with human tRNA Asp(ManQ34) and mRNA(GAU) Deposited 2022-06-22	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric	Chain x 1–63（63 aa）	Not recorded	MG MAGNESIUM ION × 248 MAN alpha-D-mannopyranose × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6；The Buffer pH was adjusted to 7.6 using KOH. cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.41 Å
7Y7F Structure of the Bacterial Ribosome with human tRNA Asp(ManQ34) and mRNA(GAC) Deposited 2022-06-22	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric	Chain x 1–63（63 aa）	Not recorded	MG MAGNESIUM ION × 259 MAN alpha-D-mannopyranose × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6；The Buffer pH was adjusted to 7.6 using KOH. cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.43 Å
7Y7G Structure of the Bacterial Ribosome with human tRNA Tyr(GalQ34) and mRNA(UAU) Deposited 2022-06-22	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric	Chain x 1–63（63 aa）	Not recorded	MG MAGNESIUM ION × 256 GAL beta-D-galactopyranose × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6；The Buffer pH was adjusted to 7.6 using KOH. cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.34 Å
7Y7H Structure of the Bacterial Ribosome with human tRNA Tyr(GalQ34) and mRNA(UAC) Deposited 2022-06-22	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric	Chain x 1–63（63 aa）	Not recorded	MG MAGNESIUM ION × 263 GAL beta-D-galactopyranose × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6；The Buffer pH was adjusted to 7.6 using KOH. cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.51 Å
7YLA Cryo-EM structure of 50S-HflX complex Deposited 2022-07-25	Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 30 PDB declaration: 32-meric	Chain g 2–63（62 aa）	Not recorded	GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 3 NA SODIUM ION × 1 ZN ZINC ION × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.52 Å
7Z20 70S E. coli ribosome with an extended uL23 loop from Candidatus marinimicrobia and a stalled filamin domain 5 nascent chain Deposited 2022-02-25	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 29 PDB declaration: 32-meric	Chain 1 1–63（63 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.29 Å
7ZOD 70S E. coli ribosome with an extended uL23 loop from Candidatus marinimicrobia Deposited 2022-04-25	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 28 PDB declaration: 31-meric	Chain 1 1–63（63 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.56 Å
7ZP8 70S E. coli ribosome with a stalled filamin domain 5 nascent chain Deposited 2022-04-26	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 29 PDB declaration: 32-meric	Chain 1 1–63（63 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.20 Å
7ZQ5 70S E. coli ribosome with truncated uL23 and uL24 loops Deposited 2022-04-29	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 28 PDB declaration: 30-meric	Chain 1 1–63（63 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.70 Å
7ZQ6 70S E. coli ribosome with truncated uL23 and uL24 loops and a stalled filamin domain 5 nascent chain Deposited 2022-04-29	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 29 PDB declaration: 32-meric	Chain 1 1–63（63 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.75 Å
7ZTA Structure of an Escherichia coli 70S ribosome stalled by Tetracenomycin X during translation of an MAAAPQK(C) peptide Deposited 2022-05-09	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 57-meric	Chain L291 2–63（62 aa）	Not recorded	MG MAGNESIUM ION × 289 K POTASSIUM ION × 115 ZN ZINC ION × 3 OCW Tetracenomycin X × 1 CYS CYSTEINE × 1 GLN GLUTAMINE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE；Waiting 30 sec Blotting 2.5 sec Blot force 5	Resolution 2.70 Å
8A3L Structural insights into the binding of bS1 to the ribosome Deposited 2022-06-08	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric	Chain x 1–63（63 aa）	Not recorded	MG MAGNESIUM ION × 313 ZN ZINC ION × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.42 Å
8AP4 Structure of Escherischia coli heat shock protein Hsp15 in complex with ribosomal 50S subunits bearing peptidyl-tRNA Deposited 2022-08-09	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 30 PDB declaration: 33-meric	Chain x 1–63（63 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.46 cryo-EM vitrification conditions Cryogen ETHANE-PROPANE	Resolution 3.00 Å
8AYE E. coli 70S ribosome bound to thermorubin and fMet-tRNA Deposited 2022-09-02	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric	Chain x 1–63（63 aa）	Not recorded	ZN ZINC ION × 2 MG MAGNESIUM ION × 308 T8B Thermorubin × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 1.96 Å
8B0X Translating 70S ribosome in the unrotated state (P and E, tRNAs) Deposited 2022-09-08	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 53-meric	Chain x 1–63（63 aa）	Not recorded	ZN ZINC ION × 1 K POTASSIUM ION × 168 MG MAGNESIUM ION × 361	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 1.55 Å
8B7Y Cryo-EM structure of the E.coli 70S ribosome in complex with the antibiotic Myxovalargin B. Deposited 2022-10-03	Different oligomeric state Different ligand/ion Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 47 PDB declaration: 52-meric	Chain g 1–63（63 aa）	Not recorded	MG MAGNESIUM ION × 237 ZN ZINC ION × 1 FME N-FORMYLMETHIONINE × 1 SPD SPERMIDINE × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE-PROPANE	Resolution 3.00 Å
8BF7 Elongating E. coli 70S ribosome containing deacylated tRNA(iMet) in the P-site and AAA mRNA codon with cognate dipeptidyl-tRNA(Lys) in the A-site Deposited 2022-10-24	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric	Chain V 1–63（63 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5；1x TAKM7 buffer cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.33 Å
8BGE Elongating E. coli 70S ribosome containing acylated tRNA(iMet) in the P-site and AAm6A mRNA codon in the A-site after uncompleted di-peptide formation Deposited 2022-10-27	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 53-meric	Chain V 1–63（63 aa）	Not recorded	FME N-FORMYLMETHIONINE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5；1x TAKM7 buffer cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.11 Å
8BGH Elongating E. coli 70S ribosome containing acylated tRNA(iMet) in the P-site and AAA mRNA codon in the A-site after uncompleted di-peptide formation Deposited 2022-10-27	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 54-meric	Chain V 1–63（63 aa）	Not recorded	FME N-FORMYLMETHIONINE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5；1x TAKM7 buffer cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.88 Å
8BH4 Elongating E. coli 70S ribosome containing deacylated tRNA(iMet) in the P-site and AAm6A mRNA codon with cognate dipeptidyl-tRNA(Lys) in the A-site Deposited 2022-10-29	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric	Chain V 1–63（63 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5；1x TAKM7 buffer cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.62 Å
8BHJ Elongating E. coli 70S ribosome containing deacylated tRNA(iMet) in the P-site and Am6AA mRNA codon with cognate dipeptidyl-tRNA(Lys) in the A-site Deposited 2022-10-31	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric	Chain V 1–63（63 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5；1x TAKM7 buffer cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.81 Å
8BHL Elongating E. coli 70S ribosome containing acylated tRNA(iMet) in the P-site and Am6AA mRNA codon in the A-site after uncompleted di-peptide formation Deposited 2022-10-31	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 53-meric	Chain V 1–63（63 aa）	Not recorded	FME N-FORMYLMETHIONINE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5；1x TAKM7 buffer cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.21 Å
8BHN Elongating E. coli 70S ribosome containing deacylated tRNA(iMet) in the P-site and m6AAA mRNA codon with cognate dipeptidyl-tRNA(Lys) in the A-site Deposited 2022-10-31	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric	Chain V 1–63（63 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5；1x TAKM7 buffer cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.85 Å
8BHP Elongating E. coli 70S ribosome containing acylated tRNA(iMet) in the P-site and m6AAA mRNA codon in the A-site after uncompleted di-peptide formation Deposited 2022-10-31	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 54-meric	Chain V 1–63（63 aa）	Not recorded	FME N-FORMYLMETHIONINE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5；1x TAKM7 buffer cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.37 Å
8BIL Initiation complex of the E. coli 70S ribosome with mRNA containing AAA codon in the A-site. Deposited 2022-11-02	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 54-meric	Chain V 1–63（63 aa）	Not recorded	FME N-FORMYLMETHIONINE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5；1x TAKM7 buffer cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.04 Å
8BIM Initiation complex of the E. coli 70S ribosome with mRNA containing AAm6A codon in the A-site Deposited 2022-11-02	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 54-meric	Chain V 1–63（63 aa）	Not recorded	FME N-FORMYLMETHIONINE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5；1x TAKM7 buffer cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.04 Å
8C8X Cryo-EM captures early ribosome assembly in action Deposited 2023-01-21	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 26 PDB declaration: 28-meric	Chain Y 1–63（63 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.93 Å
8C8Y Cryo-EM captures early ribosome assembly in action Deposited 2023-01-21	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 23 PDB declaration: 25-meric	Chain Y 1–63（63 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.03 Å
8C8Z Cryo-EM captures early ribosome assembly in action Deposited 2023-01-21	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 22 PDB declaration: 24-meric	Chain Y 1–63（63 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.12 Å
8C90 Cryo-EM captures early ribosome assembly in action Deposited 2023-01-21	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 20 PDB declaration: 22-meric	Chain Y 1–63（63 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.15 Å
8C91 Cryo-EM captures early ribosome assembly in action Deposited 2023-01-21	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 17 PDB declaration: octadecameric	Chain Y 1–63（63 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 4.19 Å
8C92 Cryo-EM captures early ribosome assembly in action Deposited 2023-01-21	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 16 PDB declaration: heptadecameric	Chain Y 1–63（63 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.79 Å
8C93 Cryo-EM captures early ribosome assembly in action Deposited 2023-01-21	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 16 PDB declaration: heptadecameric	Chain Y 1–63（63 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 4.17 Å
8C94 Cryo-EM captures early ribosome assembly in action Deposited 2023-01-21	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 19 PDB declaration: 21-meric	Chain Y 1–63（63 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.80 Å
8C95 Cryo-EM captures early ribosome assembly in action Deposited 2023-01-21	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 14 PDB declaration: hexadecameric	Chain Y 1–63（63 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 4.92 Å
8C96 Cryo-EM captures early ribosome assembly in action Deposited 2023-01-21	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 15 PDB declaration: hexadecameric	Chain Y 1–63（63 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 4.43 Å
8C97 Cryo-EM captures early ribosome assembly in action Deposited 2023-01-21	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 11 PDB declaration: dodecameric	Chain Y 1–63（63 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 4.07 Å
8C98 Cryo-EM captures early ribosome assembly in action Deposited 2023-01-21	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 11 PDB declaration: dodecameric	Chain Y 1–63（63 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.66 Å
8C99 Cryo-EM captures early ribosome assembly in action Deposited 2023-01-21	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 10 PDB declaration: undecameric	Chain Y 1–63（63 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.29 Å
8C9A Cryo-EM captures early ribosome assembly in action Deposited 2023-01-21	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 6 PDB declaration: heptameric	Chain Y 1–63（63 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 4.86 Å
8C9B Cryo-EM captures early ribosome assembly in action Deposited 2023-01-21	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 5 PDB declaration: hexameric	Chain Y 1–63（63 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 5.90 Å
8C9C Cryo-EM captures early ribosome assembly in action Deposited 2023-01-21	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 3 PDB declaration: tetrameric	Chain Y 1–63（63 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 6.62 Å
8CAM Evernimicin bound to the 50S subunit Deposited 2023-01-24	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 26 PDB declaration: 28-meric	Chain x 1–63（63 aa）	Not recorded	ZN ZINC ION × 1 ACT ACETATE ION × 1 MG MAGNESIUM ION × 246 K POTASSIUM ION × 72 6O1 Evernimicin × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 1.86 Å
8CEU Retapamulin and Capreomycin bound to the 50S subunit Deposited 2023-02-02	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 36 PDB declaration: 38-meric	Chain x 1–63（63 aa）	Not recorded	ZN ZINC ION × 1 G34 Retapamulin × 1 MG MAGNESIUM ION × 220 K POTASSIUM ION × 81	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 1.83 Å
8CGD Clindamycin bound to the 50S subunit Deposited 2023-02-03	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 28 PDB declaration: 30-meric	Chain x 1–63（63 aa）	Not recorded	ZN ZINC ION × 2 ACT ACETATE ION × 1 CLY CLINDAMYCIN × 1 AM2 APRAMYCIN × 1 MG MAGNESIUM ION × 254 K POTASSIUM ION × 85	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 1.98 Å
8CGK Lincomycin and Avilamycin bound to the 50S subunit Deposited 2023-02-05	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 26 PDB declaration: 28-meric	Chain x 1–63（63 aa）	Not recorded	ZN ZINC ION × 1 ACT ACETATE ION × 1 3QB LINCOMYCIN × 1 6UQ (2R,3S,4R,6S)-4-hydroxy-6-{[(2R,3aR,4R,4'R,5'S,6S,6'R,7aR)-4'-hydroxy-6-{[(2S,3R,4R,5S,6R)-3-hydroxy-2-{[(2R,3S,4S,5S,6S)-4-hydroxy-6-({(2R,3aS,3a'R,6S,6'R,7R,7'R,7aR,7a'R)-7'-hydroxy-7'-[(1S)-1-hydroxyethyl]-6'-methyl-7-[(2-methylpropanoyl)oxy]octahydro-4H-2,4'-spirobi[[1,3]dioxolo[4,5-c]pyran]-6-yl}oxy)-5-methoxy-2-(methoxymethyl)tetrahydro-2H-pyran-3-yl]oxy}-5-methoxy-6-methyltetrahydro-2H-pyran-4-yl]oxy}-4,6',7a-trimethyloctahydro-4H-spiro[1,3-dioxolo[4,5-c]pyran-2,2'-pyran]-5'-yl]oxy}-2-methyltetrahydro-2H-pyran-3-yl 3,5-dichloro-4-hydroxy-2-methoxy-6-methylbenzoate (non-preferred name) × 1 MG MAGNESIUM ION × 260 K POTASSIUM ION × 81	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 1.64 Å
8CGV Tiamulin bound to the 50S subunit Deposited 2023-02-06	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 27 PDB declaration: 29-meric	Chain x 1–63（63 aa）	Not recorded	ZN ZINC ION × 1 P8F Pentacycline × 2 MUL TIAMULIN × 1 MG MAGNESIUM ION × 219 K POTASSIUM ION × 83	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 1.66 Å
8HSP E. coli 70S ribosome complexed with tRNA_Ile2 bearing L34 and t6A37 in classical state Deposited 2022-12-20	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric	Chain x 1–63（63 aa）	Not recorded	MG MAGNESIUM ION × 315 K POTASSIUM ION × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6；The Buffer pH was adjusted to 7.6 using KOH. cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.32 Å
8HTZ E. coli 70S ribosome complexed with H. marismortui tRNA_Ile2 bearing agm2C34 in classical state Deposited 2022-12-22	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric	Chain x 1–63（63 aa）	Not recorded	MG MAGNESIUM ION × 317 K POTASSIUM ION × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6；The Buffer pH was adjusted to 7.6 using KOH. cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.40 Å
8HU1 E. coli 70S ribosome complexed with tRNA_Ile2 bearing L34 and ct6A37 in classical state Deposited 2022-12-22	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric	Chain x 1–63（63 aa）	Not recorded	MG MAGNESIUM ION × 314 K POTASSIUM ION × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6；The Buffer pH was adjusted to 7.6 using KOH. cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.69 Å
8IFB Dibekacin-bound E.coli 70S ribosome in the PURE system Deposited 2023-02-17	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric	Chain x 1–63（63 aa）	Not recorded	MG MAGNESIUM ION × 406 SPD SPERMIDINE × 14 84D Dibekacin × 13 SPM SPERMINE × 1 ZN ZINC ION × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.43 Å
8IFC Arbekacin-bound E.coli 70S ribosome in the PURE system Deposited 2023-02-17	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric	Chain x 1–63（63 aa）	Not recorded	MG MAGNESIUM ION × 460 SPD SPERMIDINE × 16 84G Arbekacin × 10 SPM SPERMINE × 1 ZN ZINC ION × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.90 Å
8J1Z The global structure of pre50S related to DbpA in state3 Deposited 2023-04-13	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 25 PDB declaration: 27-meric	Chain x 1–63（63 aa）	Not recorded	MG MAGNESIUM ION × 210	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5；20mM HEPES-KOH,pH7.5;140mM NH4Cl;10mM Mg2+,1mM DTT cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.60 Å
8P16 E167K RF2 on E. coli 70S release complex with UGG (Structure I) Deposited 2023-05-11	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 56-meric	Chain Y 1–63（63 aa）	Not recorded	MG MAGNESIUM ION × 330 SPD SPERMIDINE × 13 K POTASSIUM ION × 128 PUT 1,4-DIAMINOBUTANE × 13 SPM SPERMINE × 1 ZN ZINC ION × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5；HEPES-polymix buffer (pH-7.5) cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.77 Å
8P17 E167K RF2 on E. coli 70S release complex with UGG (Structure II) Deposited 2023-05-11	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 56-meric	Chain Y 1–63（63 aa）	Not recorded	MG MAGNESIUM ION × 325 SPD SPERMIDINE × 13 K POTASSIUM ION × 128 PUT 1,4-DIAMINOBUTANE × 13 SPM SPERMINE × 1 ZN ZINC ION × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5；HEPES-polymix buffer (pH-7.5) cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.78 Å
8P18 E167K RF2 on E. coli 70S release complex with UGG (Structure III) Deposited 2023-05-11	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 56-meric	Chain Y 1–63（63 aa）	Not recorded	MG MAGNESIUM ION × 321 SPD SPERMIDINE × 13 K POTASSIUM ION × 127 PUT 1,4-DIAMINOBUTANE × 13 SPM SPERMINE × 1 ZN ZINC ION × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5；HEPES-polymix buffer (pH-7.5) cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.77 Å
8PEG Escherichia coli paused disome complex (queueing 70S non-rotated closed PRE state) Deposited 2023-06-13	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 54 PDB declaration: 61-meric	Chain 2 1–63（63 aa）	Not recorded	ZN ZINC ION × 2 MG MAGNESIUM ION × 260 ATP ADENOSINE-5'-TRIPHOSPHATE × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6 cryo-EM vitrification conditions Cryogen ETHANE；Withdrawn samples were spotted directly onto freshly glow-discharged holey carbon grids, blotted for 1-2 s, and flash frozen in liquid ethane using a Vitrobot Mark IV plunger (ThermoFisher Scientific) after a wait time of 40 s at 4 degrees Celcius.	Resolution 3.30 Å
8PHJ cA4-bound Cami1 in complex with 70S ribosome Deposited 2023-06-20	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 54 PDB declaration: 61-meric	Chain x 1–63（63 aa）	Not recorded	ZN ZINC ION × 2 MG MAGNESIUM ION × 241	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.67 Å
8PKL Escherichia coli paused disome complex (leading 70S non-rotated closed PRE state) Deposited 2023-06-26	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 53 PDB declaration: 60-meric	Chain 2 1–63（63 aa）	Not recorded	ZN ZINC ION × 2 PUT 1,4-DIAMINOBUTANE × 2 SPD SPERMIDINE × 1 MG MAGNESIUM ION × 287 ALA ALANINE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6 cryo-EM vitrification conditions Cryogen ETHANE；Withdrawn samples were spotted directly onto freshly glow-discharged holey carbon grids, blotted for 1-2 s, and flash frozen in liquid ethane using a Vitrobot Mark IV plunger (ThermoFisher Scientific) after a wait time of 40 s at 4 degrees Celcius.	Resolution 3.09 Å
8PVA Structure of bacterial ribosome determined by cryoEM at 100 keV Deposited 2023-07-17	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric	Chain x 1–63（63 aa）	Not recorded	ZN ZINC ION × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 4.50 Å
8QBT E. coli ApdP-stalled ribosomal complex Deposited 2023-08-25	Different oligomeric state Different ligand/ion Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 45 PDB declaration: 52-meric	Chain Y 1–63（63 aa）	Not recorded	MG MAGNESIUM ION × 248 K POTASSIUM ION × 127 PRO PROLINE × 1 ZN ZINC ION × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE-PROPANE	Resolution 2.20 Å
8QK7 E167K RF2 on E. coli 70S release complex with UAA Deposited 2023-09-14	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 56-meric	Chain Y 1–63（63 aa）	Not recorded	MG MAGNESIUM ION × 332 PUT 1,4-DIAMINOBUTANE × 21 K POTASSIUM ION × 126 SPD SPERMIDINE × 10 SPM SPERMINE × 2 ZN ZINC ION × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5；HEPES-polymix buffer (pH-7.5) cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.77 Å
8QOA Structure of SecM-stalled Escherichia coli 70S ribosome Deposited 2023-09-28	Different oligomeric state Different ligand/ion Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 57-meric	Chain x 1–63（63 aa）	Not recorded	ZN ZINC ION × 2 K POTASSIUM ION × 132 MG MAGNESIUM ION × 288 PRO PROLINE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE-PROPANE	Resolution 2.00 Å
8R6C 70S Escherichia coli ribosome with Paenilamicin B2 bound with A- and P-site tRNA. Deposited 2023-11-22	Different oligomeric state Different ligand/ion Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 57-meric	Chain x 1–63（63 aa）	Not recorded	ZN ZINC ION × 2 MG MAGNESIUM ION × 308 K POTASSIUM ION × 3	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE-PROPANE	Resolution 2.20 Å
8R8M 70S Escherichia coli ribosome with Paenilamicin B2 bound with hybrid A/P- and hybrid P/E-tRNA. Deposited 2023-11-29	Different oligomeric state Different ligand/ion Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric	Chain x 1–63（63 aa）	Not recorded	ZN ZINC ION × 2 MG MAGNESIUM ION × 307 K POTASSIUM ION × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE-PROPANE	Resolution 2.40 Å
8RPY Escherichia coli 50S subunit in complex with the antimicrobial peptide Api137 Deposited 2024-01-17	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 31 PDB declaration: 33-meric	Chain Y 1–63（63 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.64 Å
8RPZ Escherichia coli 50S subunit in complex with the antimicrobial peptide Api88 - conformation I Deposited 2024-01-17	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 32 PDB declaration: 34-meric	Chain Y 1–63（63 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.44 Å
8RQ0 Escherichia coli 50S subunit in complex with the antimicrobial peptide Api88 - conformation II Deposited 2024-01-17	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 32 PDB declaration: 34-meric	Chain Y 1–63（63 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.44 Å
8RQ2 Escherichia coli 50S subunit in complex with the antimicrobial peptide Api88 - conformation III Deposited 2024-01-17	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 32 PDB declaration: 34-meric	Chain Y 1–63（63 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.44 Å
8W51 Structure of YchF(H114A) on E.coli 50S ribosomal subunit Deposited 2023-08-25	Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 30 PDB declaration: 32-meric	Chain Z 1–63（63 aa）	Not recorded	MG MAGNESIUM ION × 104 ZN ZINC ION × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.40 Å
8Y5K E.coli transcription translation coupling complex in TTC-A state 2 containing mRNA with 27-mer spacer, NusG, NusA, fMet-tRNA(iMet), Phe-tRNA(Phe), and viomycin Deposited 2024-01-31	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Other combination Heteromer；Protein × 59 PDB declaration: 67-meric	Chain y 1–63（63 aa）	Not recorded	MG MAGNESIUM ION × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 PO4 PHOSPHATE ION × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 6.90 Å
8Y5L E.coli transcription translation coupling complex in TTC-B state 1 (subclass1) containing mRNA with 30-mer spacer, NusG, NusA, fMet-tRNA(iMet), Phe-tRNA(Phe), and viomycin Deposited 2024-01-31	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Other combination Heteromer；Protein × 58 PDB declaration: 67-meric	Chain y 1–63（63 aa）	Not recorded	MG MAGNESIUM ION × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.70 Å
8Y5M E.coli transcription translation coupling complex in TTC-B state 2 containing mRNA with 27-mer spacer, NusG, NusA, fMet-tRNA(iMet), Phe-tRNA(Phe), and viomycin Deposited 2024-01-31	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Other combination Heteromer；Protein × 60 PDB declaration: 68-meric	Chain y 1–63（63 aa）	Not recorded	MG MAGNESIUM ION × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 PO4 PHOSPHATE ION × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 4.50 Å
8Y5N E.coli transcription translation coupling complex in TTC-A state 3 containing mRNA with 21-mer spacer, NusG, NusA, fMet-tRNA(iMet), Phe-tRNA(Phe), and viomycin Deposited 2024-01-31	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Other combination Heteromer；Protein × 59 PDB declaration: 67-meric	Chain y 1–63（63 aa）	Not recorded	GDP GUANOSINE-5'-DIPHOSPHATE × 1 PO4 PHOSPHATE ION × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 4.50 Å
8Y5O E.coli transcription translation coupling complex in TTC-B state 3 (subclass1) containing mRNA with 30-mer spacer, NusG, NusA, fMet-tRNA(iMet), Phe-tRNA(Phe), and viomycin Deposited 2024-01-31	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Other combination Heteromer；Protein × 60 PDB declaration: 68-meric	Chain y 1–63（63 aa）	Not recorded	MG MAGNESIUM ION × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 PO4 PHOSPHATE ION × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 4.20 Å
8Y5P E.coli transcription translation coupling complex in TTC-B state 4 (subclass 1) containing mRNA with 24-mer spacer, NusG, NusA, fMet-tRNA(iMet), Phe-tRNA(Phe), and viomycin Deposited 2024-01-31	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Other combination Heteromer；Protein × 60 PDB declaration: 67-meric	Chain y 1–63（63 aa）	Not recorded	MG MAGNESIUM ION × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 PO4 PHOSPHATE ION × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 6.50 Å
8Y5Q E.coli transcription translation coupling complex in TTC-B state 4 (subclass 2) containing mRNA with 27-mer spacer, NusG, NusA, fMet-tRNA(iMet), Phe-tRNA(Phe), and GDPCP Deposited 2024-01-31	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Other combination Heteromer；Protein × 59 PDB declaration: 66-meric	Chain y 1–63（63 aa）	Not recorded	MG MAGNESIUM ION × 1 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.90 Å
8Y5R E.coli Transcription translation coupling complex in TTC-B state 5 (subclass 1) containing mRNA with 27-mer spacer, NusG, NusA, fMet-tRNA(iMet), Phe-tRNA(Phe), and fusidic acid Deposited 2024-01-31	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Other combination Heteromer；Protein × 58 PDB declaration: 66-meric	Chain y 1–63（63 aa）	Not recorded	MG MAGNESIUM ION × 1 FUA FUSIDIC ACID × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 4.50 Å
8Y5S E.coli Transcription translation coupling complex in TTC-B state 5 (subclass 2) containing mRNA with 27-mer spacer, NusG, NusA, fMet-tRNA(iMet), Phe-tRNA(Phe), and GDPCP Deposited 2024-01-31	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Other combination Heteromer；Protein × 58 PDB declaration: 66-meric	Chain y 1–63（63 aa）	Not recorded	MG MAGNESIUM ION × 1 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 4.50 Å
8Y5T E.coli Transcription translation coupling complex in TTC-B state 5 (subclass 3) containing mRNA with 27-mer spacer, NusG, NusA, fMet-tRNA(iMet), Phe-tRNA(Phe), and fusidic acid Deposited 2024-01-31	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Other combination Heteromer；Protein × 58 PDB declaration: 66-meric	Chain y 1–63（63 aa）	Not recorded	MG MAGNESIUM ION × 1 FUA FUSIDIC ACID × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 4.40 Å
8YDE E.coli transcription translation coupling complex in TTC-B state 1 (subclass 3) containing mRNA with 39-mer spacer, NusG, NusA, fMet-tRNA(iMet), Phe-tRNA(Phe), and viomycin Deposited 2024-02-20	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Other combination Heteromer；Protein × 58 PDB declaration: 67-meric	Chain y 1–63（63 aa）	Not recorded	MG MAGNESIUM ION × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 5.00 Å
8YDF E.coli transcription translation coupling complex in TTC-B state 1 (subclass 2) containing mRNA with 39-mer spacer, NusG, NusA, fMet-tRNA(iMet), Phe-tRNA(Phe), and viomycin Deposited 2024-02-20	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Other combination Heteromer；Protein × 58 PDB declaration: 67-meric	Chain y 1–63（63 aa）	Not recorded	MG MAGNESIUM ION × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 4.60 Å
8YDG E.coli transcription translation coupling complex in TTC-B state 3 (subclass2) containing mRNA with 39-mer spacer, NusG, NusA, fMet-tRNA(iMet), Phe-tRNA(Phe), and viomycin Deposited 2024-02-20	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Other combination Heteromer；Protein × 60 PDB declaration: 68-meric	Chain y 1–63（63 aa）	Not recorded	MG MAGNESIUM ION × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 PO4 PHOSPHATE ION × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 5.10 Å
8YDH E.coli transcription translation coupling complex in TTC-P state 1 (subclass1) containing mRNA with 39-mer spacer, NusG, NusA, fMet-tRNA(iMet), Phe-tRNA(Phe), and viomycin Deposited 2024-02-20	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Other combination Heteromer；Protein × 58 PDB declaration: 67-meric	Chain y 1–63（63 aa）	Not recorded	MG MAGNESIUM ION × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 4.55 Å
8YDI E.coli transcription translation coupling complex in TTC-P state 1 (subclass 2) containing mRNA with 39-mer spacer, NusG, NusA, fMet-tRNA(iMet), Phe-tRNA(Phe), and viomycin Deposited 2024-02-20	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Other combination Heteromer；Protein × 58 PDB declaration: 67-meric	Chain y 1–63（63 aa）	Not recorded	MG MAGNESIUM ION × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 4.60 Å
8YDJ E.coli transcription translation coupling complex in TTC-P containing mRNA with 39-mer spacer, NusG, NusA, fMet-tRNA(iMet), Phe-tRNA(Phe), and viomycin Deposited 2024-02-20	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Other combination Heteromer；Protein × 60 PDB declaration: 68-meric	Chain y 1–63（63 aa）	Not recorded	MG MAGNESIUM ION × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 PO4 PHOSPHATE ION × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 5.10 Å
8YUO E. coli 70S ribosome complexed with P. putida tRNAIle2 at the A-site and P-site Deposited 2024-03-27	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric	Chain x 1–63（63 aa）	Not recorded	MG MAGNESIUM ION × 309	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6；The Buffer pH was adjusted to 7.6 using KOH. cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.25 Å
8YUP E. coli 70S ribosome complexed with P. putida tRNAIle2 and A4 mRNA Deposited 2024-03-28	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric	Chain x 1–63（63 aa）	Not recorded	MG MAGNESIUM ION × 309	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6；The Buffer pH was adjusted to 7.6 using KOH. cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.39 Å
8YUQ E. coli 70S ribosome complexed with P. putida tRNAIle2 and dA4 mRNA Deposited 2024-03-27	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric	Chain x 1–63（63 aa）	Not recorded	MG MAGNESIUM ION × 309	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6；The Buffer pH was adjusted to 7.6 using KOH. cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.41 Å
8YUR E. coli 70S ribosome complexed with P. putida tRNAIle2 and Am4 mRNA Deposited 2024-03-27	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric	Chain x 1–63（63 aa）	Not recorded	MG MAGNESIUM ION × 309	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6；The Buffer pH was adjusted to 7.6 using KOH. cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.47 Å
8YUS E. coli 70S ribosome complexed with P.putida tRNAIle2 and A(F)4 mRNA Deposited 2024-03-27	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric	Chain x 1–63（63 aa）	Not recorded	MG MAGNESIUM ION × 309	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6；The Buffer pH was adjusted to 7.6 using KOH. cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.43 Å
8ZEJ Structure of E.coli ribosome in complex with an engineered arrest peptide Deposited 2024-05-06	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric	Chain 1 1–63（63 aa）	Not recorded	ZN ZINC ION × 1 PRO PROLINE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6；20 mM HEPES (pH 7.6), 140 mM NH4Cl, 25 mM MgCl2, 2 mM DTT cryo-EM vitrification conditions Cryogen NITROGEN	Resolution 2.69 Å
8ZFF Structure of the Bacterial Ribosome without hypoxia-induced rRNA modifications Deposited 2024-05-07	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 54-meric	Chain x 1–63（63 aa）	Not recorded	MG MAGNESIUM ION × 457	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6；The Buffer pH was adjusted to 7.6 using KOH. cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.59 Å
8ZFG Structure of the Bacterial Ribosome with hypoxia-induced rRNA modifications Deposited 2024-05-07	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 54-meric	Chain x 1–63（63 aa）	Not recorded	MG MAGNESIUM ION × 471	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6；The Buffer pH was adjusted to 7.6 using KOH. cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.68 Å
8ZFI Structure of E.coli ribosome in complex with an engineered arrest peptide and trigger factor Deposited 2024-05-07	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 57-meric	Chain 1 1–63（63 aa）	Not recorded	ZN ZINC ION × 1 PRO PROLINE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6；20 mM HEPES (pH 7.6), 140 mM NH4Cl, 25 mM MgCl2, 2 mM DTT cryo-EM vitrification conditions Cryogen NITROGEN	Resolution 2.90 Å
8ZTU 70S ribosome arrested by PepNL Deposited 2024-06-07	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric	Chain x 1–63（63 aa）	Not recorded	MG MAGNESIUM ION × 300 K POTASSIUM ION × 47 SPD SPERMIDINE × 7 PUT 1,4-DIAMINOBUTANE × 1 ZN ZINC ION × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.90 Å
8ZTV 70S ribosome arrested by PepNL with RF2 Deposited 2024-06-07	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 57-meric	Chain x 1–63（63 aa）	Not recorded	MG MAGNESIUM ION × 301 K POTASSIUM ION × 47 SPD SPERMIDINE × 7 PUT 1,4-DIAMINOBUTANE × 1 ZN ZINC ION × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.90 Å
9CL9 WT 12C IM fraction, B-b3 with RluB bound Deposited 2024-07-10	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 11 PDB declaration: dodecameric	Chain Y 1–60（60 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5；Most of the sucrose was removed by spin concentration. cryo-EM vitrification conditions Cryogen ETHANE；3 microliter of the sample was added.	Resolution 5.04 Å
9FBV 70S Escherichia coli ribosome with P-site initiatior tRNA. Deposited 2024-05-14	Different oligomeric state Different ligand/ion Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 54-meric	Chain x 1–63（63 aa）	Not recorded	ZN ZINC ION × 2 MG MAGNESIUM ION × 306 K POTASSIUM ION × 3	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE-PROPANE	Resolution 2.40 Å
9GFT Structure of the HrpA-bound E. coli disome, Class I Deposited 2024-08-12	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 105 PDB declaration: 116-meric	Chain At 1–63（63 aa） Chain l 1–63（63 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.10 Å
9GGR Structure of the HrpA-bound E. coli disome, Class II Deposited 2024-08-14	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 106 PDB declaration: 117-meric	Chain At 1–63（63 aa） Chain l 1–63（63 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.20 Å
9GHA Fusidic acid-locked Escherichia coli 70S ribosome with Staphylococcus aureus EF-G and a tRNA in pe/E chimeric state (CHI) Deposited 2024-08-15	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric	Chain x 1–63（63 aa）	Not recorded	ZN ZINC ION × 2 K POTASSIUM ION × 94 MG MAGNESIUM ION × 271 GDP GUANOSINE-5'-DIPHOSPHATE × 1 FUA FUSIDIC ACID × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.24 Å
9GHB Fusidic acid-locked Escherichia coli 70S ribosome with Staphylococcus aureus EF-G in post-translocational state (POST) Deposited 2024-08-15	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric	Chain x 1–63（63 aa）	Not recorded	ZN ZINC ION × 2 K POTASSIUM ION × 111 MG MAGNESIUM ION × 303 GDP GUANOSINE-5'-DIPHOSPHATE × 1 FUA FUSIDIC ACID × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.21 Å
9GHC Pre-release fusidic acid-locked Escherichia coli 70S ribosome with Staphylococus aureus EF-G and FusB (FusB-EF-G-70S) Deposited 2024-08-15	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 56-meric	Chain x 1–63（63 aa）	Not recorded	ZN ZINC ION × 3 K POTASSIUM ION × 109 MG MAGNESIUM ION × 301 GDP GUANOSINE-5'-DIPHOSPHATE × 1 FUA FUSIDIC ACID × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.79 Å
9GHD Escherichia coli 70S ribosome in complex with Staphylococcus aureus FusB-EF-G (FusB-EF-G-70S*) Deposited 2024-08-15	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 56-meric	Chain x 1–63（63 aa）	Not recorded	ZN ZINC ION × 2 K POTASSIUM ION × 112 MG MAGNESIUM ION × 303	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.41 Å
9GHE Staphylococcus aureus FusB bound to the small subunit of the Escherichia coli 70S ribosome (FusB-70S:SSU) Deposited 2024-08-15	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric	Chain x 1–63（63 aa）	Not recorded	ZN ZINC ION × 3 K POTASSIUM ION × 112 MG MAGNESIUM ION × 303	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 1.87 Å
9GHF Staphylococcus aureus FusB bound to the large subunit of the Escherichia coli 70S ribosome (FusB-70S:LSU) Deposited 2024-08-15	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric	Chain x 1–63（63 aa）	Not recorded	ZN ZINC ION × 3 K POTASSIUM ION × 112 MG MAGNESIUM ION × 303	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.40 Å
9GXX 70S ribosome with doublet-decoding tRNASer3 bound to A-site GCA codon Deposited 2024-10-01	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric	Chain x 1–63（63 aa）	Not recorded	MG MAGNESIUM ION × 288 SPD SPERMIDINE × 7 PUT 1,4-DIAMINOBUTANE × 7 SPM SPERMINE × 1 ZN ZINC ION × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5；HEPES-polymix buffer (pH-7.5) cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.61 Å
9H0L 70S ribosome with cognate tRNASer3 bound to A-site AGC codon Deposited 2024-10-08	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric	Chain x 1–63（63 aa）	Not recorded	MG MAGNESIUM ION × 314 SPD SPERMIDINE × 6 SPM SPERMINE × 1 PUT 1,4-DIAMINOBUTANE × 6 ZN ZINC ION × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5；HEPES-polymix buffer (pH-7.5) cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.49 Å
9H3M 50S subunit precursor C_(L22)- Deposited 2024-10-17	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 13 PDB declaration: 14-meric	Chain Y 1–63（63 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 4.41 Å
9H3N 50S subunit precursor C_(L22)-~H61 Deposited 2024-10-17	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 12 PDB declaration: 13-meric	Chain Y 1–63（63 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.69 Å
9H3O 50S subunit precursor C_(L22)-_GAC Deposited 2024-10-17	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 16 PDB declaration: 17-meric	Chain Y 1–63（63 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 4.54 Å
9H3P 50S subunit precursor C-CP_(L22)- Deposited 2024-10-17	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 20 PDB declaration: 22-meric	Chain Y 1–63（63 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 7.06 Å
9H3Q 50S subunit precursor C-CP_YjgA_(L22)-~H61 Deposited 2024-10-17	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 21 PDB declaration: 23-meric	Chain Y 1–63（63 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 4.02 Å
9H3R 50S subunit precursor C-CP_YjgA_(L22)- Deposited 2024-10-17	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 23 PDB declaration: 25-meric	Chain Y 1–63（63 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 4.12 Å
9H3S 50S subunit precursor C-CP_YjgA_L22 Deposited 2024-10-17	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 25 PDB declaration: 27-meric	Chain Y 1–63（63 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 4.16 Å
9H3T 50S subunit precursor C_L2 Deposited 2024-10-17	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 19 PDB declaration: 20-meric	Chain Y 1–63（63 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.85 Å
9H3U 50S subunit precursor C_H68 Deposited 2024-10-17	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 19 PDB declaration: 20-meric	Chain Y 1–63（63 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.47 Å
9H3V 50S subunit precursor C-CP_L2-L28 Deposited 2024-10-17	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 23 PDB declaration: 25-meric	Chain Y 1–63（63 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.55 Å
9H3W 50S subunit precursor C-CP_H68 Deposited 2024-10-17	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 23 PDB declaration: 25-meric	Chain Y 1–63（63 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 5.38 Å
9H3X 50S subunit precursor C-CP_H68_L35 Deposited 2024-10-17	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 25 PDB declaration: 27-meric	Chain Y 1–63（63 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 4.12 Å
9H3Y 50S subunit precursor 50S_(L16)- Deposited 2024-10-17	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 26 PDB declaration: 28-meric	Chain Y 1–63（63 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.09 Å
9H3Z mature 50S subunit Deposited 2024-10-17	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 28 PDB declaration: 30-meric	Chain Y 1–63（63 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.98 Å
9HA1 Pooled 50S subunit C_(L22)- precursor states supplemented with Api137 - Canonical PET exit Api137 Deposited 2024-11-01	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 14 PDB declaration: 15-meric	Chain Y 1–63（63 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 4.17 Å
9HA2 Pooled 50S subunit C_(L22)- precursor states supplemented with Api137 - Alternative PET exit Api137 Deposited 2024-11-01	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 14 PDB declaration: 15-meric	Chain Y 1–63（63 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 4.17 Å
9HA3 Pooled 50S subunit C_(L22)-~H61 precursor states supplemented with Api137 Deposited 2024-11-01	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 13 PDB declaration: 14-meric	Chain Y 1–63（63 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.62 Å
9HA4 Pooled 50S subunit C-CP_(L22)- precursor states supplemented with Api137 Deposited 2024-11-01	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 19 PDB declaration: 21-meric	Chain Y 1–63（63 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 4.26 Å
9HA5 Pooled 50S subunit C_L2 precursor states supplemented with Api137 Deposited 2024-11-01	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 18 PDB declaration: 19-meric	Chain Y 1–63（63 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.30 Å
9HA6 mature 50S subunit supplemented with Api137 Deposited 2024-11-01	Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 30 PDB declaration: 32-meric	Chain Y 1–63（63 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.08 Å
9HA7 Pooled 50S subunit C-CP_(L22)-~H61 precursor states supplemented with Api137 Deposited 2024-11-01	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 17 PDB declaration: 19-meric	Chain Y 1–63（63 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 4.37 Å
9HAI Pooled 50S subunit C-CP_L2-L28 precursor states supplemented with Api137 Deposited 2024-11-04	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 17 PDB declaration: 18-meric	Chain Y 1–63（63 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.01 Å
9HUQ Structure of WT E.coli ribosome with complexed filament nascent chain at length 47, with P-site tRNA Deposited 2024-12-23	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 29 PDB declaration: 32-meric	Chain 1 1–63（63 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.49 Å
9HUS Structure of WT E.coli ribosome with complexed filament nascent chain at length 31, with P-site tRNAs Deposited 2024-12-23	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 29 PDB declaration: 32-meric	Chain 1 1–63（63 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.40 Å
9HY6 Structure of E.coli ribosome with nascent chain at linker length of 31 amino acids, with mRNA, P-site and A-site tRNAs Deposited 2025-01-09	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 29 PDB declaration: 34-meric	Chain 1 1–63（63 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.60 Å
9HYM Structure of WT E.coli ribosome with complexed filament nascent chain at length 34, with mRNA, P-site and A-site tRNAs, and mRNA Deposited 2025-01-10	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 29 PDB declaration: 34-meric	Chain 1 1–63（63 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.20 Å
9I3L Structure of E.coli ribosome with filamin mutant Y719E nascent chain at linker length of 47 amino acids, with tRNA Deposited 2025-01-23	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 29 PDB declaration: 32-meric	Chain 1 1–63（63 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.10 Å
9IOT Cryo-EM structure of Escherichia coli hibernating ribosome with RNase I mutant Deposited 2024-07-09	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 53 PDB declaration: 56-meric	Chain 0 1–63（63 aa）	Not recorded	MG MAGNESIUM ION × 475 CA CALCIUM ION × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6；20 mM HEPES-KOH (pH 7.6), 10 mM Mg(OAc)2, 30 mM NH4Cl, and 6 mM 2-mercaptoethanol cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.70 Å
9JMK 50S Ribosomal Subunit precursor state III Deposited 2024-09-20	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 26 PDB declaration: 28-meric	Chain Y 1–63（63 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 4.00 Å
9JNS 50S precursor - Erm complex (C-II) Deposited 2024-09-23	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 17 PDB declaration: octadecameric	Chain Y 1–63（63 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 4.70 Å
9JPM Structure of the Bacterial Ribosome with human tRNA Lys(mcm5s2U34) and mRNA(AAA) Deposited 2024-09-26	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric	Chain x 1–63（63 aa）	Not recorded	MG MAGNESIUM ION × 391 ZN ZINC ION × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6；The Buffer pH was adjusted to 7.6 using KOH. cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.43 Å
9JPO Structure of the Bacterial Ribosome with human tRNA Lys(mcm5h2U34) and mRNA(AAA) Deposited 2024-09-26	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric	Chain x 1–63（63 aa）	Not recorded	MG MAGNESIUM ION × 448 ZN ZINC ION × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6；The Buffer pH was adjusted to 7.6 using KOH. cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.18 Å
9JPP Structure of the Bacterial Ribosome with human tRNA Lys(mcm5s2U34) and mRNA(AAG) Deposited 2024-09-26	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric	Chain x 1–63（63 aa）	Not recorded	MG MAGNESIUM ION × 443 ZN ZINC ION × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6；The Buffer pH was adjusted to 7.6 using KOH. cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.26 Å
9JQ2 Structure of the Bacterial Ribosome with human tRNA Lys(mcm5h2U34) and mRNA(AAG) Deposited 2024-09-27	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric	Chain x 1–63（63 aa）	Not recorded	MG MAGNESIUM ION × 438 ZN ZINC ION × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6；The Buffer pH was adjusted to 7.6 using KOH. cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.61 Å
9JSR 50S precursor - Erm complex (C-I) Deposited 2024-10-01	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 17 PDB declaration: octadecameric	Chain Y 1–63（63 aa）	Not recorded	AN6 5'-{[(3S)-3-amino-3-carboxypropyl](ethyl)amino}-5'-deoxyadenosine × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 4.00 Å
9N2B Impacts of ribosomal RNA sequence variation on gene expression and phenotype: Cryo-EM structure of the rrsB ribosome (BBB-70S) Deposited 2025-01-28	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 54-meric	Chain Lc 1–63（63 aa）	Not recorded	PUT 1,4-DIAMINOBUTANE × 9 SPD SPERMIDINE × 5 MG MAGNESIUM ION × 276 K POTASSIUM ION × 56 ZN ZINC ION × 3 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 FME N-FORMYLMETHIONINE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.20 Å
9N2C Impacts of ribosomal RNA sequence variation on gene expression and phenotype: Cryo-EM structure of the rrsH ribosome (HBB-70S) Deposited 2025-01-28	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 54-meric	Chain Lc 1–63（63 aa）	Not recorded	PUT 1,4-DIAMINOBUTANE × 10 SPD SPERMIDINE × 5 MG MAGNESIUM ION × 283 K POTASSIUM ION × 49 ZN ZINC ION × 3 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 FME N-FORMYLMETHIONINE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.40 Å
9Q87 Principles of ion binding to RNA inferred from the analysis of a 1.55 Angstrom resolution bacterial ribosome structure - Part I: Mg2+ Deposited 2025-02-23	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric	Chain x 1–63（63 aa）	Not recorded	K POTASSIUM ION × 235 MG MAGNESIUM ION × 415 CU1 COPPER (I) ION × 5 ZN ZINC ION × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 1.55 Å
9QQQ Cryo-EM structure of SKM-70S ribosomal stalled complex in the major state (vacant A-site, canon) Deposited 2025-04-01	Different oligomeric state Different ligand/ion Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric	Chain x 1–63（63 aa）	Not recorded	ZN ZINC ION × 2 A1JA7 [(2~{S},3~{S},4~{R},5~{R},6~{S})-4-[(2~{S},3~{R},4~{S},5~{R},6~{S})-5-acetamido-6-(hydroxymethyl)-4-[(2~{R},3~{R},4~{S},5~{S},6~{R})-6-(hydroxymethyl)-4-[(2~{S},3~{R},4~{R},5~{R},6~{R})-6-(hydroxymethyl)-5-methoxy-3,4-bis(oxidanyl)oxan-2-yl]oxy-3,5-bis(oxidanyl)oxan-2-yl]oxy-3-oxidanyl-oxan-2-yl]oxy-6-carbamimidamido-2-(hydroxymethyl)-5-oxidanyl-oxan-3-yl] carbamate × 1 MG MAGNESIUM ION × 312	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE-PROPANE	Resolution 2.26 Å
9QSJ Cryo-EM structure of SKM-70S ribosomal stalled complex in the A-tRNA positioned (Body open) state. Deposited 2025-04-05	Different oligomeric state Different ligand/ion Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric	Chain x 1–63（63 aa）	Not recorded	ZN ZINC ION × 2 A1JAI [(2~{S},3~{S},4~{R},5~{R},6~{S})-4-[(2~{S},3~{R},4~{S},5~{R},6~{S})-5-acetamido-6-(hydroxymethyl)-4-[(2~{R},3~{R},4~{S},5~{S},6~{R})-6-(hydroxymethyl)-4-[(2~{S},3~{R},4~{R},5~{R},6~{R})-6-(hydroxymethyl)-5-methoxy-3,4-bis(oxidanyl)oxan-2-yl]oxy-3,5-bis(oxidanyl)oxan-2-yl]oxy-3-oxidanyl-oxan-2-yl]oxy-2-(hydroxymethyl)-6-[[~{N}-[4-[(~{N}-methylcarbamimidoyl)amino]butyl]carbamimidoyl]amino]-5-oxidanyl-oxan-3-yl] carbamate × 1 MG MAGNESIUM ION × 311	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE-PROPANE	Resolution 2.62 Å
9R3A Structure of Stalled Beta-Galactosidase 70S Ribosome Nascent Chain Deposited 2025-05-03	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric	Chain x 1–63（63 aa）	Not recorded	ZN ZINC ION × 2 K POTASSIUM ION × 131 MG MAGNESIUM ION × 287 PRO PROLINE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.2 cryo-EM vitrification conditions Cryogen ETHANE-PROPANE	Resolution 2.86 Å
9RBF Structure of a stalled E. coli 70S RNC-NuoK-86 in complex with the membrane protein insertase SecYEG-YidC Deposited 2025-05-22	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 54 PDB declaration: 61-meric	Chain x 1–63（63 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY mmCIF provides none of the parsed conditions	Resolution 2.44 Å
9RFW Manikomycin bound to the Escherichia coli 50S ribosomal subunit Deposited 2025-06-05	Different oligomeric state Different ligand/ion Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 31 PDB declaration: 35-meric	Chain x 1–63（63 aa）	Not recorded	ZN ZINC ION × 2 MG MAGNESIUM ION × 213 K POTASSIUM ION × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE-PROPANE	Resolution 2.40 Å
9RJA Manikomycin bound to the Escherichia coli 70S ribosome Deposited 2025-06-12	Different oligomeric state Different ligand/ion Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 57-meric	Chain x 1–63（63 aa）	Not recorded	ZN ZINC ION × 2 MG MAGNESIUM ION × 309 K POTASSIUM ION × 2 PAR PAROMOMYCIN × 1 SPD SPERMIDINE × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE-PROPANE	Resolution 2.45 Å
9RTU Structure of the 70S-EF-G(P610L)-GDP-Pi ribosome complex with tRNAs in hybrid state 1 (H1-EF-G(P610L)-GDP-Pi) Deposited 2025-07-03	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 53 PDB declaration: 59-meric	Chain Y 1–63（63 aa）	Not recorded	MG MAGNESIUM ION × 369 ZN ZINC ION × 2 NA SODIUM ION × 2 AM2 APRAMYCIN × 3 PO4 PHOSPHATE ION × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5；50 mM HEPES, 70 mM NH4Cl, 30 mM KCl, 3.5 mM MgCl2, 0.6 mM spermine, 0.4 mM spermidine cryo-EM vitrification conditions Cryogen ETHANE；Manual blotting & plunge-freezing	Resolution 3.00 Å
9RTV Structure of the 70S-EF-G(P610L)-GDP-Pi ribosome complex with tRNAs in hybrid state 2 (H2-EF-G(P610L)-GDP-Pi) Deposited 2025-07-03	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 53 PDB declaration: 59-meric	Chain Y 1–63（63 aa）	Not recorded	MG MAGNESIUM ION × 369 ZN ZINC ION × 2 NA SODIUM ION × 2 AM2 APRAMYCIN × 3 GDP GUANOSINE-5'-DIPHOSPHATE × 1 PO4 PHOSPHATE ION × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5；50 mM HEPES, 70 mM NH4Cl, 30 mM KCl, 3.5 mM MgCl2, 0.6 mM spermine, 0.4 mM spermidine cryo-EM vitrification conditions Cryogen ETHANE；Manual blotting & plunge-freezing	Resolution 3.60 Å
9SLG Doxycycline Bound E. coli Ribosome with Rearranged Peptidyl Transferase Centre Deposited 2025-09-03	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 54-meric	Chain x 1–63（63 aa）	Not recorded	ZN ZINC ION × 2 MG MAGNESIUM ION × 447 K POTASSIUM ION × 77 DXT (4S,4AR,5S,5AR,6R,12AS)-4-(DIMETHYLAMINO)-3,5,10,12,12A-PENTAHYDROXY-6-METHYL-1,11-DIOXO-1,4,4A,5,5A,6,11,12A-OCTAHYDROTETRACENE-2-CARBOXAMIDE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5；BS100 ribosomal resuspension buffer cryo-EM vitrification conditions Cryogen ETHANE；Blot time 4 s, blot force 1, blot total 1	Resolution 2.16 Å
9SRO Cryo-EM structure of SKM-70S ribosomal stalled complex in the rotated state with hybrid tRNAs Deposited 2025-09-24	Different oligomeric state Different ligand/ion Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 54-meric	Chain x 1–63（63 aa）	Not recorded	ZN ZINC ION × 2 MG MAGNESIUM ION × 309 A1JAI [(2~{S},3~{S},4~{R},5~{R},6~{S})-4-[(2~{S},3~{R},4~{S},5~{R},6~{S})-5-acetamido-6-(hydroxymethyl)-4-[(2~{R},3~{R},4~{S},5~{S},6~{R})-6-(hydroxymethyl)-4-[(2~{S},3~{R},4~{R},5~{R},6~{R})-6-(hydroxymethyl)-5-methoxy-3,4-bis(oxidanyl)oxan-2-yl]oxy-3,5-bis(oxidanyl)oxan-2-yl]oxy-3-oxidanyl-oxan-2-yl]oxy-2-(hydroxymethyl)-6-[[~{N}-[4-[(~{N}-methylcarbamimidoyl)amino]butyl]carbamimidoyl]amino]-5-oxidanyl-oxan-3-yl] carbamate × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE-PROPANE	Resolution 2.60 Å
9SS4 pre-50S ribosomal subunit in rimM-KO with RsfS (-H68) Deposited 2025-09-25	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 33 PDB declaration: 35-meric	Chain B2 1–63（63 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.70 Å
9SS5 50S ribosomal subunit in rimM-KO with RsfS (+H68) Deposited 2025-09-25	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 32 PDB declaration: 34-meric	Chain B2 1–63（63 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.70 Å
9SS6 70S ribosome from RimM-KO Deposited 2025-09-25	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 55-meric	Chain B2 1–63（63 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.60 Å
9SYH E. coli 70S ribosome from deltaRlmE strain Deposited 2025-10-12	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric	Chain x 1–63（63 aa）	Not recorded	K POTASSIUM ION × 148 MG MAGNESIUM ION × 351 SR0 N~1~-(3-azaniumylpropyl)butane-1,4-diaminium × 6 PUT 1,4-DIAMINOBUTANE × 9 ZN ZINC ION × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5；HEPES-polymix buffer (pH-7.5) cryo-EM vitrification conditions Cryogen ETHANE	Resolution 1.89 Å
9T0Y E. coli 70S ribosome from delta-9 strain Deposited 2025-10-20	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric	Chain x 1–63（63 aa）	Not recorded	K POTASSIUM ION × 143 MG MAGNESIUM ION × 337 SR0 N~1~-(3-azaniumylpropyl)butane-1,4-diaminium × 6 PUT 1,4-DIAMINOBUTANE × 9 ZN ZINC ION × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5；HEPES-polymix buffer (pH-7.5) cryo-EM vitrification conditions Cryogen ETHANE	Resolution 1.87 Å
9T6M E. coli 70S ribosome from delta-10 strain Deposited 2025-11-07	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric	Chain x 1–63（63 aa）	Not recorded	K POTASSIUM ION × 132 MG MAGNESIUM ION × 316 SR0 N~1~-(3-azaniumylpropyl)butane-1,4-diaminium × 5 PUT 1,4-DIAMINOBUTANE × 8 ZN ZINC ION × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5；HEPES-polymix buffer (pH-7.5) cryo-EM vitrification conditions Cryogen ETHANE	Resolution 1.87 Å
9TEW DalDro bound to the initiating Escherichia coli 70S ribosome Deposited 2025-11-26	Different oligomeric state Different ligand/ion Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric	Chain x 1–63（63 aa）	Not recorded	ZN ZINC ION × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE-PROPANE	Resolution 2.90 Å
9TEX Dal2 bound to the Escherichia coli 70S ribosome Deposited 2025-11-26	Different oligomeric state Different ligand/ion Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric	Chain x 1–63（63 aa）	Not recorded	ZN ZINC ION × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE-PROPANE	Resolution 2.54 Å
9TEZ DalDroS bound to the Escherichia coli 50S ribosomal subunit Deposited 2025-11-26	Different oligomeric state Different ligand/ion Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 31 PDB declaration: 34-meric	Chain x 1–63（63 aa）	Not recorded	ZN ZINC ION × 2 SPM SPERMINE × 1 MG MAGNESIUM ION × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE-PROPANE	Resolution 2.88 Å
9TMI E. coli 70S ribosome with A- and P-site tRNA Deposited 2025-12-13	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric	Chain x 1–63（63 aa）	Not recorded	ZN ZINC ION × 2 MG MAGNESIUM ION × 258 SCM SPECTINOMYCIN × 1 VAL VALINE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.08 Å
9TNL E. coli 70S ribosome with A/P- and P/E-site tRNA Deposited 2025-12-16	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric	Chain x 1–63（63 aa）	Not recorded	ZN ZINC ION × 2 SCM SPECTINOMYCIN × 1 MG MAGNESIUM ION × 266 K POTASSIUM ION × 52 VAL VALINE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.11 Å
9TQA E. coli 70S ribosome, trapped conformational ground state of SSU-h44 apical loop, with A- and P-site tRNA Deposited 2025-12-19	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric	Chain x 1–63（63 aa）	Not recorded	ZN ZINC ION × 2 SCM SPECTINOMYCIN × 1 MG MAGNESIUM ION × 238 VAL VALINE × 1 K POTASSIUM ION × 6	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.06 Å
9WNQ Structure of E.coli ribosome in complex with an engineered arrest peptide Deposited 2025-09-05	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric	Chain 1 1–63（63 aa）	Not recorded	ZN ZINC ION × 1 PRO PROLINE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6；20 mM HEPES (pH 7.6), 140 mM NH4Cl, 25 mM MgCl2, 2 mM DTT cryo-EM vitrification conditions Cryogen NITROGEN	Resolution 2.59 Å
9WNR Structure of E.coli ribosome in complex with an engineered arrest peptide and trigger factor Deposited 2025-09-05	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 57-meric	Chain 1 1–63（63 aa）	Not recorded	ZN ZINC ION × 1 PRO PROLINE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6；20 mM HEPES (pH 7.6), 140 mM NH4Cl, 25 mM MgCl2, 2 mM DTT cryo-EM vitrification conditions Cryogen NITROGEN	Resolution 2.76 Å
9XFK In situ structure of bacterial 50S ribosomes Deposited 2025-10-29	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 29 PDB declaration: 31-meric	Chain g 2–63（62 aa）	Not recorded	MG MAGNESIUM ION × 166 NA SODIUM ION × 1 ZN ZINC ION × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.98 Å
9XFL In vitro structure of bacterial 50S ribosomes Deposited 2025-10-29	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 29 PDB declaration: 31-meric	Chain g 2–63（62 aa）	Not recorded	MG MAGNESIUM ION × 176 NA SODIUM ION × 1 ZN ZINC ION × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.7 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.16 Å
9ZTL Single Particle Cryo EM Analysis of a Ribosome Nascent Globin Complex Deposited 2025-12-23	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric	Chain x 1–63（63 aa）	Not recorded	ZN ZINC ION × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.91 Å

457 other PDB entries and 502 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence

UniProt name	RL29_ECOLI
Isoform	—
PDB entities	30
Chains and sequence ranges	Author chain x； PDBConstruct 1–63； UniProt 1–63

50S ribosomal protein L30

OrganismNot specified

UniProt C3SR32

State in the Current Structure

Assembly	Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Associated Components	Method and Experimental Conditions	Structure Quality
1	Protein–RNA Heteromer Protein × 30 RNA 2 PDB declaration: 32-meric（32） Consistent with all polymer counts	Chain y； UniProt 1–59	Not recorded	50S ribosomal protein L33 × 1 （A0A3S5YPG8） 50S ribosomal protein L34 × 1 （A0A8X6EVB8） 50S ribosomal protein L35 × 1 （A0A8S0FT45） 50S ribosomal protein L36 × 1 （A0A2X1PTA3） 50S ribosomal protein L31 × 1 （C3SIV2） Drosocin1 × 1 （B4MNS1） 23S ribosomal RNA × 1 5S ribosomal RNA × 1 50S ribosomal protein L2 × 1 （A0A7L6YWH1） 50S ribosomal protein L3 × 1 （C3SQU2） 50S ribosomal protein L4 × 1 （D8A1N0） 50S ribosomal protein L5 × 1 （D6IEK6） 50S ribosomal protein L6 × 1 （C3SR17） 50S ribosomal protein L9 × 1 （C3SFP2） 50S ribosomal protein L13 × 1 （A0A656YGQ7） 50S ribosomal protein L14 × 1 （C3SQZ2） 50S ribosomal protein L15 × 1 （P02413） 50S ribosomal protein L16 × 1 （A0A0H3EMH6） 50S ribosomal protein L17 × 1 （A0A1X3JBW6） 50S ribosomal protein L18 × 1 （A0A514EKV0） 50S ribosomal protein L19 × 1 （A0A370V6W8） 50S ribosomal protein L20 × 1 （A0A378BCJ4） 50S ribosomal protein L21 × 1 （A0A0E2L2H1） 50S ribosomal protein L22 × 1 （A0A0F6B7N1） 50S ribosomal protein L23 × 1 （D7Y3D8） 50S ribosomal protein L24 × 1 （S1E9P3） 50S ribosomal protein L25 × 1 （P68919） 50S ribosomal protein L27 × 1 （C3SSG7） 50S ribosomal protein L28 × 1 （C3SME2） 50S ribosomal protein L29 × 1 （P0A7M6） 50S ribosomal protein L32 × 1 （A0A657HW13） ZN ZINC ION × 2 A2G 2-acetamido-2-deoxy-alpha-D-galactopyranose × 1 MG MAGNESIUM ION × 216 SPM SPERMINE × 1	ELECTRON MICROSCOPY cryo-EM buffer：pH 7.4 cryo-EM vitrification conditions：Cryogen ETHANE-PROPANE	Resolution 2.10 Å

Other States of the Same Protein in the Database

Other PDB	Difference from Current Entry 8ANA	Assembly / Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Non-polymers	Method and Experimental Conditions	Structure Quality
11BQ Escherichia coli 70S ribosome containing an evolved 16S rRNA (EC-S3.5) Deposited 2026-02-16	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric	Chain y 1–59（59 aa）	Not recorded	ZN ZINC ION × 2 MG MAGNESIUM ION × 307 SPD SPERMIDINE × 14 SPM SPERMINE × 1 C CYTIDINE-5'-MONOPHOSPHATE × 1 G GUANOSINE-5'-MONOPHOSPHATE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.00 Å
11DG Chimeric Escherichia coli 70S ribosome containing an evolved Vibrio cholerae 16S rRNA (VC-S4.4) Deposited 2026-02-18	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric	Chain y 1–59（59 aa）	Not recorded	ZN ZINC ION × 2 MG MAGNESIUM ION × 272 SPD SPERMIDINE × 14 SPM SPERMINE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.00 Å
11EL Chimeric Escherichia coli 70S ribosome containing an evolved 16S rRNA from Vibrio cholerae (VC-ST) Deposited 2026-02-19	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric	Chain y 1–59（59 aa）	Not recorded	ZN ZINC ION × 2 MG MAGNESIUM ION × 308 SPD SPERMIDINE × 16 SPM SPERMINE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.20 Å
11FV Chimeric Escherichia coli 70S ribosome containing an evolved 16S rRNA from Pseudomonas aeruginosa (PA-S3.3) Deposited 2026-02-21	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 52-meric	Chain h 1–59（59 aa）	Not recorded	MG MAGNESIUM ION × 307	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.90 Å
11GG Chimeric Escherichia coli 70S ribosome containing an evolved 16S rRNA from Pseudomonas aeruginosa (PA-ST) Deposited 2026-02-22	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 52-meric	Chain h 1–59（59 aa）	Not recorded	MG MAGNESIUM ION × 307	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.02 Å
4V85 Crystal Structure of Release Factor RF3 Trapped in the GTP State on a Rotated Conformation of the Ribosome. Deposited 2011-06-13	Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 55 PDB declaration: 59-meric	Chain B3 1–59（59 aa）	Not recorded	MG MAGNESIUM ION × 493 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；295 K；Tris Ac PH.7.0, 25-35 mM KCL, 6.1% PEG 20000, 1% glycerol, 50mM sucrose, VAPOR DIFFUSION, SITTING DROP, temperature 295K	Resolution 3.20 Å R-free 0.250
8AKN Cryo-EM structure of the proline-rich antimicrobial peptide drosocin bound to the terminating ribosome Deposited 2022-07-30	Different oligomeric state Different ligand/ion Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 57-meric	Chain y 1–59（59 aa）	Not recorded	ZN ZINC ION × 2 A2G 2-acetamido-2-deoxy-alpha-D-galactopyranose × 1 MG MAGNESIUM ION × 305 SPM SPERMINE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE-PROPANE	Resolution 2.30 Å
8AM9 Cryo-EM structure of the proline-rich antimicrobial peptide drosocin bound to the elongating ribosome Deposited 2022-08-03	Different oligomeric state Different ligand/ion Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric	Chain y 1–59（59 aa）	Not recorded	ZN ZINC ION × 2 MG MAGNESIUM ION × 293 SPM SPERMINE × 1 A2G 2-acetamido-2-deoxy-alpha-D-galactopyranose × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE-PROPANE	Resolution 2.80 Å
8FZF Cryo-EM structure of an E. coli rotated ribosome complex bound with RF3-ppGpp and p/E-tRNAPhe (Composite state I-C) Deposited 2023-01-28	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 56-meric	Chain 2 1–59（59 aa）	Not recorded	MG MAGNESIUM ION × 384 G4P GUANOSINE-5',3'-TETRAPHOSPHATE × 1 ZN ZINC ION × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4；5 mM Tris-HCl, 60 mM NH4Cl, 10 mM MgCl2, 6 mM B-mercaptoethanol cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.20 Å
8URM E. coli 70S ribosome with unmodified tRNAPro(GGG) bound to slippery P-site CCC-C codon and tRNAVal(UAC) in the A site Deposited 2023-10-26	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric	Chain Z 1–59（59 aa）	Not recorded	MG MAGNESIUM ION × 395	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.00 Å
8URY Escherichia coli transcription-translation coupled complex class B (TTC-B) containing RfaH bound to ops signal, NusA, mRNA with a 30 nt long spacer, and fMet-tRNA in E-site and P-site of the ribosome Deposited 2023-10-27	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Other combination Heteromer；Protein × 60 PDB declaration: 68-meric	Chain f 1–59（59 aa）	Not recorded	MG MAGNESIUM ION × 1 ZN ZINC ION × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.10 Å
8UTJ E. coli 70S ribosome with unmodified lys-tRNAPro(GGG) bound to slippery P-site CCC-C codon in the 0 frame Deposited 2023-10-31	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric	Chain Z 1–59（59 aa）	Not recorded	MG MAGNESIUM ION × 530 LYS LYSINE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.50 Å
8UX8 E. coli 70S ribosome with unmodified Lys-tRNAPro(GGG) bound to slippery P-site CCC-C codon in the +1 mRNA reading frame Deposited 2023-11-09	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric	Chain Z 1–59（59 aa）	Not recorded	MG MAGNESIUM ION × 395 LYS LYSINE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.60 Å
8V03 E. coli 70S ribosome with unmodified P/E-tRNAPro(GGG) bound to slippery P-site CCC-C codon Deposited 2023-11-16	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 53-meric	Chain Z 1–59（59 aa）	Not recorded	MG MAGNESIUM ION × 281	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 4.00 Å
8VOS Escherichia coli transcription-translation loosely coupled complex (TTC-LC) containing mRNA with a 60 nt long spacer, NusG, NusA, and fMet-tRNAs in E-site and P-site Deposited 2024-01-15	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Other combination Heteromer；Protein × 60 PDB declaration: 68-meric	Chain f 1–59（59 aa）	Not recorded	MG MAGNESIUM ION × 1 ZN ZINC ION × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 4.60 Å
8VS9 Endogenous trans-translation complex with tmRNA*SmpB in the P site and alanyl-tRNA in the A site and deacyl-tRNA in the E site of E. coli 70S ribosome Deposited 2024-01-23	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 53 PDB declaration: 59-meric	Chain L30 1–59（59 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.90 Å
8VSA Endogenous trans-translation complex with tmRNA*SmpB in the P site and alanyl-tRNA in the A site of E. coli 70S ribosome Deposited 2024-01-23	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 53 PDB declaration: 58-meric	Chain L30 1–59（59 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.70 Å
8VV4 E. coli 70S ribosome with unmodified Lys-tRNAPro(GGG) in the P/P conformation on a slippery CCC-C codon Deposited 2024-01-30	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 54-meric	Chain Z 1–59（59 aa）	Not recorded	MG MAGNESIUM ION × 424 LYS LYSINE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.40 Å
8VWQ E. coli 70S ribosome with unmodified Lys-tRNAPro(GGG) in the P/P conformation on a slippery CCC-C codon and Elongation Factor P bound (uL1 in the closed conformation) Deposited 2024-02-02	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 56-meric	Chain Z 1–59（59 aa）	Not recorded	MG MAGNESIUM ION × 586 LYS LYSINE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.10 Å
8W2N E. coli 70S ribosome with unmodified Lys-tRNAPro(GGG) in the P/P conformation on a slippery CCC-C codon and Elongation Factor P bound (uL1 in the open conformation) Deposited 2024-02-20	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric	Chain Z 1–59（59 aa）	Not recorded	MG MAGNESIUM ION × 481	ELECTRON MICROSCOPY cryo-EM buffer pH 7.6 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.20 Å
9AX7 70S initiation complex (tRNA-fMet M1 + CUG start codon) Deposited 2024-03-06	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 54-meric	Chain y 1–59（59 aa）	Not recorded	ZN ZINC ION × 2 MG MAGNESIUM ION × 309 IAS BETA-L-ASPARTIC ACID × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.63 Å
9B4Z E. coli 70S ribosome complex (N1-methylated 16S A1408 + arbekacin) Deposited 2024-03-21	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric	Chain BZ 1–59（59 aa）	Not recorded	MG MAGNESIUM ION × 309 84G Arbekacin × 2 ZN ZINC ION × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.20 Å
9B50 E. coli 70S ribosome complex (unmethylated 16S A1408 + arbekacin) Deposited 2024-03-21	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 52-meric	Chain BZ 1–59（59 aa）	Not recorded	MG MAGNESIUM ION × 309 84G Arbekacin × 2 ZN ZINC ION × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.70 Å
9B51 E. coli 70S ribosome complex (N1-methylated 16S A1408 + G418) Deposited 2024-03-21	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric	Chain BZ 1–59（59 aa）	Not recorded	MG MAGNESIUM ION × 309 GET GENETICIN × 2 ZN ZINC ION × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.40 Å
9CG5 70S initiation complex (tRNA-fMet M1 + UUG start codon) Deposited 2024-06-28	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 54-meric	Chain y 1–59（59 aa）	Not recorded	ZN ZINC ION × 2 MG MAGNESIUM ION × 309 IAS BETA-L-ASPARTIC ACID × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.59 Å
9CG6 70S initiation complex (tRNA-fMet M1 + GUG start codon) Deposited 2024-06-28	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 54-meric	Chain y 1–59（59 aa）	Not recorded	ZN ZINC ION × 2 MG MAGNESIUM ION × 309 IAS BETA-L-ASPARTIC ACID × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.61 Å
9CG7 70S initiation complex (tRNA-fMet M1 + AUG start codon) Deposited 2024-06-28	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 54-meric	Chain y 1–59（59 aa）	Not recorded	ZN ZINC ION × 2 MG MAGNESIUM ION × 309 IAS BETA-L-ASPARTIC ACID × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.75 Å
9MKK Structure of arbekacin bound Escherichia coli 70S ribosome Deposited 2024-12-17	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 47 PDB declaration: 52-meric	Chain y 1–59（59 aa）	Not recorded	84G Arbekacin × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.20 Å
9MOR Damaged 70S ribosome with PrfH bound Deposited 2024-12-27	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 58-meric	Chain Z 1–59（59 aa）	Not recorded	MG MAGNESIUM ION × 439 ZN ZINC ION × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.65 Å
9MQ4 Damaged 70S ribosome with PrfH bound Deposited 2025-01-01	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 58-meric	Chain Z 1–59（59 aa）	Not recorded	MG MAGNESIUM ION × 439 ZN ZINC ION × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.78 Å
9O2X Structure of WT E.coli ribosome 70S subunit with complexed with mRNA, P-site fMet-NH-tRNAfMet and A-site (S)-betahydroxyBocK charged NH-tRNAPyl Deposited 2025-04-04	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric	Chain y 1–59（59 aa）	Not recorded	ZN ZINC ION × 2 MG MAGNESIUM ION × 320 FME N-FORMYLMETHIONINE × 1 K POTASSIUM ION × 10 PAR PAROMOMYCIN × 1 SPD SPERMIDINE × 14 SPM SPERMINE × 1 A1B71 (2S)-6-[(tert-butoxycarbonyl)amino]-2-(hydroxymethyl)hexanoic acid × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.29 Å
9O2Y Structure of WT E.coli ribosome 70S subunit with complexed with mRNA, P-site fMet-NH-tRNAfMet and A-site (R) beta-2-hydroxy-BocLysine acid charged NH-tRNAPyl Deposited 2025-04-04	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric	Chain y 1–59（59 aa）	Not recorded	PAR PAROMOMYCIN × 1 MG MAGNESIUM ION × 320 SPD SPERMIDINE × 13 FME N-FORMYLMETHIONINE × 1 SPM SPERMINE × 1 K POTASSIUM ION × 10 ZN ZINC ION × 2 A1B70 (2R)-6-[(tert-butoxycarbonyl)amino]-2-(hydroxymethyl)hexanoic acid × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.14 Å
9PIH E. coli 70S ribosome bound to Doxycycline Deposited 2025-07-10	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 54-meric	Chain y 1–59（59 aa）	Not recorded	ZN ZINC ION × 1 MG MAGNESIUM ION × 276 K POTASSIUM ION × 92 DXT (4S,4AR,5S,5AR,6R,12AS)-4-(DIMETHYLAMINO)-3,5,10,12,12A-PENTAHYDROXY-6-METHYL-1,11-DIOXO-1,4,4A,5,5A,6,11,12A-OCTAHYDROTETRACENE-2-CARBOXAMIDE × 6	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.40 Å
9PII E. coli 70S ribosome bound to Sarecycline Deposited 2025-07-10	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 54-meric	Chain y 1–59（59 aa）	Not recorded	ZN ZINC ION × 1 MG MAGNESIUM ION × 281 K POTASSIUM ION × 100 V7A Sarecycline × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.40 Å
9PIJ E. coli 70S ribosome bound to Minocycline Deposited 2025-07-10	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 54-meric	Chain y 1–59（59 aa）	Not recorded	ZN ZINC ION × 1 MG MAGNESIUM ION × 340 K POTASSIUM ION × 148 MIY (4S,4AS,5AR,12AS)-4,7-BIS(DIMETHYLAMINO)-3,10,12,12A-TETRAHYDROXY-1,11-DIOXO-1,4,4A,5,5A,6,11,12A-OCTAHYDROTETRACENE-2- CARBOXAMIDE × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.20 Å
9Y79 Escherichia coli transcription-translation loosely coupled complex (TTC-LC^walked) containing mRNA with a 39 nt long spacer, NusG, NusA, and fMet-tRNAs in E-site and P-site Deposited 2025-09-09	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Other combination Heteromer；Protein × 60 PDB declaration: 68-meric	Chain f 1–59（59 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY mmCIF provides none of the parsed conditions	Resolution 3.20 Å

36 other PDB entries and 36 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence

UniProt name	C3SR32_ECOLX
Isoform	—
PDB entities	31
Chains and sequence ranges	Author chain y； PDBConstruct 1–59； UniProt 1–59

50S ribosomal protein L32

OrganismNot specified

UniProt A0A657HW13

State in the Current Structure

Assembly	Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Associated Components	Method and Experimental Conditions	Structure Quality
1	Protein–RNA Heteromer Protein × 30 RNA 2 PDB declaration: 32-meric（32） Consistent with all polymer counts	Chain z； UniProt 1–57	Not recorded	50S ribosomal protein L33 × 1 （A0A3S5YPG8） 50S ribosomal protein L34 × 1 （A0A8X6EVB8） 50S ribosomal protein L35 × 1 （A0A8S0FT45） 50S ribosomal protein L36 × 1 （A0A2X1PTA3） 50S ribosomal protein L31 × 1 （C3SIV2） Drosocin1 × 1 （B4MNS1） 23S ribosomal RNA × 1 5S ribosomal RNA × 1 50S ribosomal protein L2 × 1 （A0A7L6YWH1） 50S ribosomal protein L3 × 1 （C3SQU2） 50S ribosomal protein L4 × 1 （D8A1N0） 50S ribosomal protein L5 × 1 （D6IEK6） 50S ribosomal protein L6 × 1 （C3SR17） 50S ribosomal protein L9 × 1 （C3SFP2） 50S ribosomal protein L13 × 1 （A0A656YGQ7） 50S ribosomal protein L14 × 1 （C3SQZ2） 50S ribosomal protein L15 × 1 （P02413） 50S ribosomal protein L16 × 1 （A0A0H3EMH6） 50S ribosomal protein L17 × 1 （A0A1X3JBW6） 50S ribosomal protein L18 × 1 （A0A514EKV0） 50S ribosomal protein L19 × 1 （A0A370V6W8） 50S ribosomal protein L20 × 1 （A0A378BCJ4） 50S ribosomal protein L21 × 1 （A0A0E2L2H1） 50S ribosomal protein L22 × 1 （A0A0F6B7N1） 50S ribosomal protein L23 × 1 （D7Y3D8） 50S ribosomal protein L24 × 1 （S1E9P3） 50S ribosomal protein L25 × 1 （P68919） 50S ribosomal protein L27 × 1 （C3SSG7） 50S ribosomal protein L28 × 1 （C3SME2） 50S ribosomal protein L29 × 1 （P0A7M6） 50S ribosomal protein L30 × 1 （C3SR32） ZN ZINC ION × 2 A2G 2-acetamido-2-deoxy-alpha-D-galactopyranose × 1 MG MAGNESIUM ION × 216 SPM SPERMINE × 1	ELECTRON MICROSCOPY cryo-EM buffer：pH 7.4 cryo-EM vitrification conditions：Cryogen ETHANE-PROPANE	Resolution 2.10 Å

Other States of the Same Protein in the Database

Other PDB	Difference from Current Entry 8ANA	Assembly / Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Non-polymers	Method and Experimental Conditions	Structure Quality
8AKN Cryo-EM structure of the proline-rich antimicrobial peptide drosocin bound to the terminating ribosome Deposited 2022-07-30	Different oligomeric state Different ligand/ion Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 57-meric	Chain z 1–57（57 aa）	Not recorded	ZN ZINC ION × 2 A2G 2-acetamido-2-deoxy-alpha-D-galactopyranose × 1 MG MAGNESIUM ION × 305 SPM SPERMINE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE-PROPANE	Resolution 2.30 Å
8AM9 Cryo-EM structure of the proline-rich antimicrobial peptide drosocin bound to the elongating ribosome Deposited 2022-08-03	Different oligomeric state Different ligand/ion Different structure-quality metrics	Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric	Chain z 1–57（57 aa）	Not recorded	ZN ZINC ION × 2 MG MAGNESIUM ION × 293 SPM SPERMINE × 1 A2G 2-acetamido-2-deoxy-alpha-D-galactopyranose × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE-PROPANE	Resolution 2.80 Å

2 other PDB entries and 2 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence

UniProt name	A0A657HW13_SALET
Isoform	—
PDB entities	32
Chains and sequence ranges	Author chain z； PDBConstruct 1–57； UniProt 1–57

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

SAXS scattering curve SAXS Profile

P(r) Distance Distribution P(r) Distribution

Download Download SAXS (.dat) P(r) (.pr.out) CIF

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2. Structure Basics 2. Structure Basics

Entry ID entry_id	8ana
Deposition date deposition_date	2022-08-05
Structure title title	Cryo-EM structure of the proline-rich antimicrobial peptide drosocin bound to the 50S ribosomal subunit
Keywords keywords	E.coli, Drosocin, Dro1, antimicrobial peptide, proline-rich, PrAMP, 50S, RIBOSOME; RIBOSOME
Experimental Method method	ELECTRON MICROSCOPY

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3. SAXS Parameters (CRYSOL theoretical calculation) 3. SAXS Parameters (CRYSOL)

Radius of gyration Rg (Guinier) rg_guinier	67.60 Å
Radius of gyration Rg (electron density) rg_electron	68.53 Å
Forward intensity I(0) i0	55589400000.00
Molecular weight molecular_weight	1288400.0 kDa
Excluded volume excluded_volume	1315000 Å³
Envelope volume envelope_volume	2153000 Å³
Hydration-shell volume shell_volume	238990 Å³
Envelope diameter envelope_diameter	234.8 Å
Shell Rg shell_rg	81.27 Å
Envelope Rg envelope_rg	68.69 Å
Shape Rg shape_rg	68.59 Å
Total Rg total_rg	68.52 Å
Total atoms total_atoms	86419
Residues n_residues	6028
Spherical-harmonic order n_harmonics	20
q range q_range	— – 0.5000 Å⁻¹
Data points n_points	101
Shell type shell_type	directional
Solvent electron density solvent_density	0.3340 e/Å³
Shell contrast contrast_shell	0.0300 e/Å³
CRYSOL version crysol_version	4.1.3

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4. P(r) Distance Distribution (GNOM inversion) 4. P(r) Analysis (GNOM)

Maximum dimension Dmax dmax	226.5 Å
Rg (real space) rg_real	67.42 Å
Rg uncertainty (real space) rg_real_error	2.02 Å
I(0) (real space) i0_real	5.5590e+10
I(0) uncertainty (real space) i0_real_error	1.1360e+09
Rg (reciprocal space) rg_reciprocal	68.14 Å
I(0) (reciprocal space) i0_reciprocal	55660000000.0000
Solution quality estimate total_estimate	0.8564
Solution quality rating solution_quality	GOOD a GOOD solution
P(r) peaks n_peaks	1
Primary peak position r_peak_primary	88.7 Å
Skewness Skewness skewness	0.239
Kurtosis Kurtosis kurtosis	-0.359
Angular range angular_range	— – 0.1150 Å⁻¹
Current regularization parameter α current_alpha	0.0000
Highest regularization parameter α highest_alpha	8777000000.0000
Real-space data points n_real_points	24
GNOM version gnom_version	4.1.3
Quality Criteria quality_criteria	AN1: 0.000; Oscil: 0.803; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.966; Smooth: 0.753

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5. Crystallography and Experiment 5. Crystallography & Experiment

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6. Entities and Polymers Entities & Polymers (37)

Entity 1 Entity 1 polymer

Description description	50S ribosomal protein L33
Molecular weight formula_weight	6388.6 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	0
Atom count atom_count	86461

MAKGIREKIKLVSSAGTGHFYTTTKNKRTKPEKLELKKFDPVVRQHVIYKEAKIK

Entity 2 Entity 2 polymer

Description description	50S ribosomal protein L34
Molecular weight formula_weight	5397.5 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	1

MKRTFQPSVLKRNRSHGFRARMATKNGRQVLARRRAKGRARLTVSK

Entity 3 Entity 3 polymer

Description description	50S ribosomal protein L35
Molecular weight formula_weight	7313.0 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	2

MPKIKTVRGAAKRFKKTGKGGFKHKHANLRHILTKKATKRKRHLRPKAMVSKGDLGLVIACLPYA

Entity 4 Entity 4 polymer

Description description	50S ribosomal protein L36
Molecular weight formula_weight	4377.4 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	3

MKVRASVKKLCRNCKIVKRDGVIRVICSAEPKHKQRQG

Entity 5 Entity 5 polymer

Description description	50S ribosomal protein L31
Molecular weight formula_weight	7887.1 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	4

MKKDIHPKYEEITASCSCGNVMKIRSTVGHDLNLDVCSKCHPFFTGKQRDVATGGRVDRFNKRFNIPGSK

Entity 6 Entity 6 polymer

Description description	Drosocin1
Molecular weight formula_weight	2204.6 kDa
Source method src_method	syn
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	A

GKPRPYSPRPTSHPRPIRV

Entity 7 Entity 7 polymer

Description description	23S ribosomal RNA
Molecular weight formula_weight	941463.4 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polyribonucleotide
Chain IDs strand_id	a

GGUUAAGCGACUAAGCGUACACGGUGGAUGCCCUGGCAGUCAGAGGCGAUGAAGGACGUGCUAAUCUGCGAUAAGCGUCGGUAAGGUGAUAUGAACCGUUAUAACCGGCGAUUUCCGAAUGGGGAAACCCAGUGUGUUUCGACACACUAUCAUUAACUGAAUCCAUAGGUUAAUGAGGCGAACCGGGGGAACUGAAACAUCUAAGUACCCCGAGGAAAAGAAAUCAACCGAGAUUCCCCCAGUAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCUGAAUCAGUGUGUGUGUUAGUGGAAGCGUCUGGAAAGGCGCGCGAUACAGGGUGACAGCCCCGUACACAAAAAUGCACAUGCUGUGAGCUCGAUGAGUAGGGCGGGACACGUGGUAUCCUGUCUGAAUAUGGGGGGACCAUCCUCCAAGGCUAAAUACUCCUGACUGACCGAUAGUGAACCAGUACCGUGAGGGAAAGGCGAAAAGAACCCCGGCGAGGGGAGUGAAAAAGAACCUGAAACCGUGUACGUACAAGCAGUGGGAGCACGCUUAGGCGUGUGACUGCGUACCUUUUGUAUAAUGGGUCAGCGACUUAUAUUCUGUAGCAAGGUUAACCGAAUAGGGGAGCCGAAGGGAAACCGAGUCUUAACUGGGCGUUAAGUUGCAGGGUAUAGACCCGAAACCCGGUGAUCUAGCCAUGGGCAGGUUGAAGGUUGGGUAACACUAACUGGAGGACCGAACCGACUAAUGUUGAAAAAUUAGCGGAUGACUUGUGGCUGGGGGUGAAAGGCCAAUCAAACCGGGAGAUAGCUGGUUCUCCCCGAAAGCUAUUUAGGUAGCGCCUCGUGAAUUCAUCUCCGGGGGUAGAGCACUGUUUCGGCAAGGGGGUCAUCCCGACUUACCAACCCGAUGCAAACUGCGAAUACCGGAGAAUGUUAUCACGGGAGACACACGGCGGGUGCUAACGUCCGUCGUGAAGAGGGAAACAACCCAGACCGCCAGCUAAGGUCCCAAAGUCAUGGUUAAGUGGGAAACGAUGUGGGAAGGCCCAGACAGCCAGGAUGUUGGCUUAGAAGCAGCCAUCAUUUAAAGAAAGCGUAAUAGCUCACUGGUCGAGUCGGCCUGCGCGGAAGAUGUAACGGGGCUAAACCAUGCACCGAAGCUGCGGCAGCGACGCUUAUGCGUUGUUGGGUAGGGGAGCGUUCUGUAAGCCUGCGAAGGUGUGCUGUGAGGCAUGCUGGAGGUAUCAGAAGUGCGAAUGCUGACAUAAGUAACGAUAAAGCGGGUGAAAAGCCCGCUCGCCGGAAGACCAAGGGUUCCUGUCCAACGUUAAUCGGGGCAGGGUGAGUCGACCCCUAAGGCGAGGCCGAAAGGCGUAGUCGAUGGGAAACAGGUUAAUAUUCCUGUACUUGGUGUUACUGCGAAGGGGGGACGGAGAAGGCUAUGUUGGCCGGGCGACGGUUGUCCCGGUUUAAGCGUGUAGGCUGGUUUUCCAGGCAAAUCCGGAAAAUCAAGGCUGAGGCGUGAUGACGAGGCACUACGGUGCUGAAGCAACAAAUGCCCUGCUUCCAGGAAAAGCCUCUAAGCAUCAGGUAACAUCAAAUCGUACCCCAAACCGACACAGGUGGUCAGGUAGAGAAUACCAAGGCGCUUGAGAGAACUCGGGUGAAGGAACUAGGCAAAAUGGUGCCGUAACUUCGGGAGAAGGCACGCUGAUAUGUAGGUGAGGUCCCUCGCGGAUGGAGCUGAAAUCAGUCGAAGAUACCAGCUGGCUGCAACUGUUUAUUAAAAACACAGCACUGUGCAAACACGAAAGUGGACGUAUACGGUGUGACGCCUGCCCGGUGCCGGAAGGUUAAUUGAUGGGGUUAGCGCAAGCGAAGCUCUUGAUCGAAGCCCCGGUAAACGGCGGCCGUAACUAUAACGGUCCUAAGGUAGCGAAAUUCCUUGUCGGGUAAGUUCCGACCUGCACGAAUGGCGUAAUGAUGGCCAGGCUGUCUCCACCCGAGACUCAGUGAAAUUGAACUCGCUGUGAAGAUGCAGUGUACCCGCGGCAAGACGGAAAGACCCCGUGAACCUUUACUAUAGCUUGACACUGAACAUUGGACCUUGAUGUGUAGGAUAGGUGGGAGGCUUUGAAGUGUGGACGCCAGUCUGCAUGGAGCCGACCUUGAAAUACCACCCUUUAAUGUUUGAUGUUCUAACGUUGACCCCUAAUCGGGGUUGCGGACAGUGUCUGGUGGGUAGUUUGACUGGGGCGGUCUCCUCCUAAAGAGUAACGGAGGAGCACGAAGGUUGGCUAAUCCUGGUCGGACAUCAGGAGGUUAGUGCAAUGGCAUAAGCCAGCUUGACUGCGAGCGUGACGGCGCGAGCAGGUGCGAAAGCAGGUCAUAGUGAUCCGGUGGUUCUGAAUGGAAGGGCCAUCGCUCAACGGAUAAAAGGUACUCCGGGGAUAACAGGCUGAUACCGCCCAAGAGUUCAUAUCGACGGCGGUGUUUGGCACCUCGAUGUCGGCUCAUCACAUCCUGGGGCUGAAGUAGGUCCCAAGGGUAUGGCUGUUCGCCAUUUAAAGUGGUACGCGAGCUGGGUUUAGAACGUCGUGAGACAGUUCGGUCCCUAUCUGCCGUGGGCGCUGGAGAACUGAGGGGGGCUGCUCCUAGUACGAGAGGACCGGAGUGGACGCAUCACUGGUGUUCGGGUUGUCAUGCCAAUGGCACUGCCCGGUAGCUAAAUGCGGAAGAGAUAAGUGCUGAAAGCAUCUAAGCACGAAACUUGCCCCGAGAUGAGUUCUCCCUGACCCUUUAAGGGUCCUGAAGGAACGUUGAAGACGACGACGUUGAUAGGCCGGGUGUGUAAGCGCAGCGAUGCGUUGAGCUAACCGGUACUAAUGAACCGUGAGGCUUAACCU

Entity 8 Entity 8 polymer

Description description	5S ribosomal RNA
Molecular weight formula_weight	38790.1 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polyribonucleotide
Chain IDs strand_id	b

UGCCUGGCGGCCGUAGCGCGGUGGUCCCACCUGACCCCAUGCCGAACUCAGAAGUGAAACGCCGUAGCGCCGAUGGUAGUGUGGGGUCUCCCCAUGCGAGAGUAGGGAACUGCCAGGCAU

Entity 9 Entity 9 polymer

Description description	50S ribosomal protein L2
Molecular weight formula_weight	29923.6 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	c

MAVVKCKPTSPGRRHVVKVVNPELHKGKPFAPLLEKNSKSGGRNNNGRITTRHIGGGHKQAYRIVDFKRNKDGIPAVVERLEYDPNRSANIALVLYKDGERRYILAPKGLKAGDQIQSGVDAAIKPGNTLPMRNIPVGSTVHNVEMKPGKGGQLARSAGTYVQIVARDGAYVTLRLRSGEMRKVEADCRATLGEVGNAEHMLRVLGKAGAARWRGVRPTVRGTAMNPVDHPHGGGEGRNFGKHPVTPWGVQTKGKKTRSNKRTDKFIVRRRSK

Entity 10 Entity 10 polymer

Description description	50S ribosomal protein L3
Molecular weight formula_weight	22291.6 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	d

MIGLVGKKVGMTRIFTEDGVSIPVTVIEVEANRVTQVKDLANDGYRAIQVTTGAKKANRVTKPEAGHFAKAGVEAGRGLWEFRLAEGEEFTVGQSISVELFADVKKVDVTGTSKGKGFAGTVKRWNFRTQDATHGNSLSHRVPGSIGQNQTPGKVFKGKKMAGQMGNERVTVQSLDVVRVDAERNLLLVKGAVPGATGSDLIVKPAVKA

Entity 11 Entity 11 polymer

Description description	50S ribosomal protein L4
Molecular weight formula_weight	22121.6 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	e

MELVLKDAQSALTVSETTFGRDFNEALVHQVVVAYAAGARQGTRAQKTRAEVTGSGKKPWRQKGTGRARSGSIKSPIWRSGGVTFAARPQDHSQKVNKKMYRGALKSILSELVRQDRLIVVEKFSVEAPKTKLLAQKLKDMALEDVLIITGELDENLFLAARNLHKVDVRDATGIDPVSLIAFDKVVMTADAVKQVEEMLA

Entity 12 Entity 12 polymer

Description description	50S ribosomal protein L5
Molecular weight formula_weight	20333.6 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	f

MAKLHDYYKDEVVKKLMTEFNYNSVMQVPRVEKITLNMGVGEAIADKKLLDNAAADLAAISGQKPLITKARKSVAGFKIRQGYPIGCKVTLRGERMWEFFERLITIAVPRIRDFRGLSAKSFDGRGNYSMGVREQIIFPEIDYDKVDRVRGLDITITTTAKSDEEGRALLAAFDFPFRK

Entity 13 Entity 13 polymer

Description description	50S ribosomal protein L6
Molecular weight formula_weight	18932.8 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	g

MSRVAKAPVVVPAGVDVKINGQVITIKGKNGELTRTLNDAVEVKHADNTLTFGPRDGYADGWAQAGTARALLNSMVIGVTEGFTKKLQLVGVGYRAAVKGNVINLSLGFSHPVDHQLPAGITAECPTQTEIVLKGADKQVIGQVAADLRAYRRPEPYKGKGVRYADEVVRTKEAKKK

Entity 14 Entity 14 polymer

Description description	50S ribosomal protein L9
Molecular weight formula_weight	15789.0 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	h

MQVILLDKVANLGSLGDQVNVKAGYARNFLVPQGKAVPATKKNIEFFEARRAELEAKLAEVLAAANARAEKINALETVTIASKAGDEGKLFGSIGTRDIADAVTAAGVEVAKSEVRLPNGVLRTTGEHEVSFQVHSEVFAKVIVNVVAE

Entity 15 Entity 15 polymer

Description description	50S ribosomal protein L13
Molecular weight formula_weight	16050.6 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	i

MKTFTAKPETVKRDWYVVDATGKTLGRLATELARRLRGKHKAEYTPHVDTGDYIIVLNADKVAVTGNKRTDKVYYHHTGHIGGIKQATFEEMIARRPERVIEIAVKGMLPKGPLGRAMFRKLKVYAGNEHNHAAQQPQVLDI

Entity 16 Entity 16 polymer

Description description	50S ribosomal protein L14
Molecular weight formula_weight	13565.1 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	j

MIQEQTMLNVADNSGARRVMCIKVLGGSHRRYAGVGDIIKITIKEAIPRGKVKKGDVLKAVVVRTKKGVRRPDGSVIRFDGNACVLLNNNSEQPIGTRIFGPVTRELRSEKFMKIISLAPEVL

Entity 17 Entity 17 polymer

Description description	50S ribosomal protein L15
Molecular weight formula_weight	15008.5 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	k

MRLNTLSPAEGSKKAGKRLGRGIGSGLGKTGGRGHKGQKSRSGGGVRRGFEGGQMPLYRRLPKFGFTSRKAAITAEIRLSDLAKVEGGVVDLNTLKAANIIGIQIEFAKVILAGEVTTPVTVRGLRVTKGARAAIEAAGGKIEE

Entity 18 Entity 18 polymer

Description description	50S ribosomal protein L16
Molecular weight formula_weight	15312.3 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	l

MLQPKRTKFRKMHKGRNRGLAQGTDVSFGSFGLKAVGRGRLTARQIEAARRAMTRAVKRQGKIWIRVFPDKPITEKPLAVRMGKGKGNVEYWVALIQPGKVLYEMDGVPEELAREAFKLAAAKLPIKTTFVTKTVM

Entity 19 Entity 19 polymer

Description description	50S ribosomal protein L17
Molecular weight formula_weight	14393.7 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	m

MRHRKSGRQLNRNSSHRQAMFRNMAGSLVRHEIIKTTLPKAKELRRVVEPLITLAKTDSVANRRLAFARTRDNEIVAKLFNELGPRFASRAGGYTRILKCGFRAGDNAPMAYIELVDRSEKAEAAAE

Entity 20 Entity 20 polymer

Description description	50S ribosomal protein L18
Molecular weight formula_weight	12794.7 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	n

MDKKSARIRRATRARRKLQELGATRLVVHRTPRHIYAQVIAPNGSEVLVAASTVEKAIAEQLKYTGNKDAAAAVGKAVAERALEKGIKDVSFDRSGFQYHGRVQALADAAREAGLQF

Entity 21 Entity 21 polymer

Description description	50S ribosomal protein L19
Molecular weight formula_weight	13159.3 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	o

MSNIIKQLEQEQMKQDVPSFRPGDTVEVKVWVVEGSKKRLQAFEGVVIAIRNRGLHSAFTVRKISNGEGVERVFQTHSPVVDSISVKRRGAVRKAKLYYLRERTGKAARIKERLN

Entity 22 Entity 22 polymer

Description description	50S ribosomal protein L20
Molecular weight formula_weight	13528.0 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	p

MARVKRGVIARARHKKILKQAKGYYGARSRVYRVAFQAVIKAGQYAYRDRRQRKRQFRQLWIARINAAARQNGISYSKFINGLKKASVEIDRKILADIAVFDKVAFTALVEKAKAALA

Entity 23 Entity 23 polymer

Description description	50S ribosomal protein L21
Molecular weight formula_weight	11586.4 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	q

MYAVFQSGGKQHRVSEGQTVRLEKLDIATGETVEFAEVLMIANGEEVKIGVPFVDGGVIKAEVVAHGRGEKVKIVKFRRRKHYRKQQGHRQWFTDVKITGISA

Entity 24 Entity 24 polymer

Description description	50S ribosomal protein L22
Molecular weight formula_weight	12253.4 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	r

METIAKHRHARSSAQKVRLVADLIRGKKVSQALDILTYTNKKAAVLVKKVLESAIANAEHNDGADIDDLKVTKIFVDEGPSMKRIMPRAKGRADRILKRTSHITVVVSDR

Entity 25 Entity 25 polymer

Description description	50S ribosomal protein L23
Molecular weight formula_weight	11222.2 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	s

MIREERLLKVLRAPHVSEKASTAMEKSNTIVLKVAKDATKAEIKAAVQKLFEVEVEVVNTLVVKGKVKRHGQRIGRRSDWKKAYVTLKEGQNLDFVGGAE

Entity 26 Entity 26 polymer

Description description	50S ribosomal protein L24
Molecular weight formula_weight	11339.2 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	t

MAAKIRRDDEVIVLTGKDKGKRGKVKNVLSSGKVIVEGINLVKKHQKPVPALNQPGGIVEKEAAIQVSNVAIFNAATGKADRVGFRFEDGKKVRFFKSNSETIK

Entity 27 Entity 27 polymer

Description description	50S ribosomal protein L25
Molecular weight formula_weight	10713.5 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	u

MFTINAEVRKEQGKGASRRLRAANKFPAIIYGGKEAPLAIELDHDKVMNMQAKAEFYSEVLTIVVDGKEIKVKAQDVQRHPYKPKLQHIDFVRA

Entity 28 Entity 28 polymer

Description description	50S ribosomal protein L27
Molecular weight formula_weight	9146.5 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	v

MAHKKAGGSTRNGRDSEAKRLGVKRFGGESVLAGSIIVRQRGTKFHAGANVGCGRDHTLFAKADGKVKFEVKGPKNRKFISIEAE

Entity 29 Entity 29 polymer

Description description	50S ribosomal protein L28
Molecular weight formula_weight	9027.6 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	w

MSRVCQVTGKRPVTGNNRSHALNATKRRFLPNLHSHRFWVESEKRFVTLRVSAKGMRVIDKKGIDTVLAELRARGEKY

Entity 30 Entity 30 polymer

Description description	50S ribosomal protein L29
Molecular weight formula_weight	7286.5 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	x

MKAKELREKSVEELNTELLNLLREQFNLRMQAASGQLQQSHLLKQVRRDVARVKTLLNEKAGA

Entity 31 Entity 31 polymer

Description description	50S ribosomal protein L30
Molecular weight formula_weight	6554.8 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	y

MAKTIKITQTRSAIGRLPKHKATLLGLGLRRIGHTVEREDTPAIRGMINAVSFMVKVEE

Entity 32 Entity 32 polymer

Description description	50S ribosomal protein L32
Molecular weight formula_weight	6463.4 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	z

MAVQQNKPTRSKRGMRRSHDALTAVTSLSVDKTSGEKHLRHHITADGYYRGRKVIAK

Entity 33 Entity 33 non-polymer

Description description	ZINC ION
Molecular weight formula_weight	65.4 kDa
Source method src_method	syn
Copy count entity_count	2

Entity 34 Entity 34 non-polymer

Description description	2-acetamido-2-deoxy-alpha-D-galactopyranose
Molecular weight formula_weight	221.2 kDa
Source method src_method	syn
Copy count entity_count	1

Entity 35 Entity 35 non-polymer

Description description	MAGNESIUM ION
Molecular weight formula_weight	24.3 kDa
Source method src_method	syn
Copy count entity_count	216

Entity 36 Entity 36 non-polymer

Description description	SPERMINE
Molecular weight formula_weight	202.3 kDa
Source method src_method	syn
Copy count entity_count	1

Entity 37 Entity 37 water

Description description	water
Molecular weight formula_weight	18.0 kDa
Source method src_method	nat
Copy count entity_count	42

▼

7. Fold Classification (SCOP + CATH) 4 domains

CATH v4.4 (4 domains)

Domain ID domain_id	8anag01
Class class	3 — Alpha Beta
Architecture architecture	90 — Alpha-Beta Complex
Topology topology	930 — Outer Surface Protein A; domain 3
Homologous superfamily homologous superfamily	12 — Ribosomal protein L6

Domain ID domain_id	8anag02
Class class	3 — Alpha Beta
Architecture architecture	90 — Alpha-Beta Complex
Topology topology	930 — Outer Surface Protein A; domain 3
Homologous superfamily homologous superfamily	12 — Ribosomal protein L6

Domain ID domain_id	8anak01
Class class	3 — Alpha Beta
Architecture architecture	100 — Ribosomal Protein L15; Chain: K; domain 2
Topology topology	10 — Ribosomal Protein L15; Chain: K; domain 2
Homologous superfamily homologous superfamily	10 —

Domain ID domain_id	8anar01
Class class	3 — Alpha Beta
Architecture architecture	90 — Alpha-Beta Complex
Topology topology	470 — Ribosomal Protein L22; Chain A
Homologous superfamily homologous superfamily	10 — Ribosomal protein L22/L17

▶

8. Citations (1)

▶

Unit-cell length a length_a	1.000 Å
Unit-cell length b length_b	1.000 Å
Unit-cell length c length_c	1.000 Å
Unit-cell angle α angle_alpha	90.00 °
Unit-cell angle β angle_beta	90.00 °
Unit-cell angle γ angle_gamma	90.00 °
Molecules per unit cell Z z_pdb	—

Space group space_group	P 1
Space-group number int_tables_number	1
Crystal system crystal_system	—

Resolution resolution	2.100 Å
Low-resolution limit resolution_low	2.100 Å
R factor r_factor	0.3186
R_work r_work	0.3186
R_free r_free	—

Resolution resolution	2.10 Å
Resolution测定方法 resolution_method	—
颗粒数 num_particles	159749

fit	/app/data/saxs_computation/an/8ana/8ana.fit
pddf	/app/data/pr_computation/an/8ana/8ana.pr.out
profile	/app/data/saxs_computation/an/8ana/8ana.dat