Current Protein Identity：F1SU49
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Difference tags compare only the current result set; every original PDB and assembly record remains separate.
Related-Structure Differences
Each row represents one biological assembly in one PDB entry; multiple monomers of the same protein are listed separately.
| PDB Entry | Assembly / Oligomeric State | Construct | Mutations and Modifications | Ligands, Ions and Non-polymers | Experimental Method | Experimental Conditions | Structure Quality |
|---|---|---|---|---|---|---|---|
| 5AJ3 Structure of the small subunit of the mammalian mitoribosome Deposited 2015-02-20 | Assembly 1 Protein–RNA Heteromer；Protein × 33 PDB declaration: 37-meric（37） Consistent with all polymers |
Chain m
1–118（118 aa）
|
Not recorded | MG MAGNESIUM ION × 144 ZN ZINC ION × 3 GDP GUANOSINE-5'-DIPHOSPHATE × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
20 MM HEPES-KOH, 50 MM KCL, 40 MM MGCL2, 1 MM DTT；pH 7.4；20 MM HEPES-KOH, 50 MM KCL, 40 MM MGCL2, 1 MM DTT
cryo-EM vitrification conditions
Cryogen ETHANE-PROPANE；MIXTURE OF LIQUID ETHANE AND PROPANE
|
Resolution 3.60 Å |
| 5AJ4 Structure of the 55S mammalian mitoribosome. Deposited 2015-02-20 | Assembly 1 Protein–RNA Heteromer；Protein × 83 PDB declaration: 89-meric（89） Consistent with all polymers |
Chain Am
2–117（116 aa）
|
Not recorded | MG MAGNESIUM ION × 351 ZN ZINC ION × 6 GDP GUANOSINE-5'-DIPHOSPHATE × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
20 MM HEPES-KOH, 50 MM KCL, 40 MM MGCL2, 1 MM DTT；pH 7.4；20 MM HEPES-KOH, 50 MM KCL, 40 MM MGCL2, 1 MM DTT
cryo-EM vitrification conditions
Cryogen ETHANE-PROPANE；MIXTURE OF LIQUID ETHANE AND PROPANE
|
Resolution 3.80 Å |
| 6GAW Unique features of mammalian mitochondrial translation initiation revealed by cryo-EM. This file contains the complete 55S ribosome. Deposited 2018-04-13 | Assembly 1 Protein–RNA Heteromer；Protein × 90 PDB declaration: 95-meric（95） Consistent with all polymers |
Chain Am
1–118（118 aa）
|
Not recorded | MG MAGNESIUM ION × 326 ZN ZINC ION × 6 5GP GUANOSINE-5'-MONOPHOSPHATE × 2 SPM SPERMINE × 3 GSP 5'-GUANOSINE-DIPHOSPHATE-MONOTHIOPHOSPHATE × 1 NA SODIUM ION × 1 FME N-FORMYLMETHIONINE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.20 Å |
| 6GAZ Unique features of mammalian mitochondrial translation initiation revealed by cryo-EM. This file contains the 28S ribosomal subunit. Deposited 2018-04-13 | Assembly 1 Protein–RNA Heteromer；Protein × 33 PDB declaration: 36-meric（36） Consistent with all polymers |
Chain Am
1–118（118 aa）
|
Not recorded | GSP 5'-GUANOSINE-DIPHOSPHATE-MONOTHIOPHOSPHATE × 1 MG MAGNESIUM ION × 111 NA SODIUM ION × 1 SPM SPERMINE × 1 ZN ZINC ION × 3 FME N-FORMYLMETHIONINE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.10 Å |
| 6YDP 55S mammalian mitochondrial ribosome with mtEFG1 and P site fMet-tRNAMet (POST) Deposited 2020-03-20 | Assembly 1 Protein–RNA Heteromer；Protein × 89 PDB declaration: 94-meric（94） Consistent with all polymers |
Chain Am
1–118（118 aa）
|
Not recorded | MG MAGNESIUM ION × 326 ZN ZINC ION × 6 SPM SPERMINE × 3 FME N-FORMYLMETHIONINE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 5GP GUANOSINE-5'-MONOPHOSPHATE × 2 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE-PROPANE
|
Resolution 3.00 Å |
| 6YDW 55S mammalian mitochondrial ribosome with mtEFG1 and two tRNAMet (TI-POST) Deposited 2020-03-21 | Assembly 1 Protein–RNA Heteromer；Protein × 89 PDB declaration: 95-meric（95） Consistent with all polymers |
Chain Am
1–118（118 aa）
|
Not recorded | MG MAGNESIUM ION × 325 ZN ZINC ION × 6 SPM SPERMINE × 3 FME N-FORMYLMETHIONINE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 5GP GUANOSINE-5'-MONOPHOSPHATE × 1 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE-PROPANE
|
Resolution 4.20 Å |
| 7NQH 55S mammalian mitochondrial ribosome with mtRF1a and P-site tRNAMet Deposited 2021-03-01 | Assembly 1 Protein–RNA Heteromer；Protein × 88 PDB declaration: 93-meric（93） Consistent with all polymers |
Chain Am
1–118（118 aa）
|
Not recorded | MG MAGNESIUM ION × 323 ZN ZINC ION × 6 SPM SPERMINE × 3 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 5GP GUANOSINE-5'-MONOPHOSPHATE × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE-PROPANE
|
Resolution 3.50 Å |
| 7NQL 55S mammalian mitochondrial ribosome with ICT1 and P site tRNAMet Deposited 2021-03-01 | Assembly 1 Protein–RNA Heteromer；Protein × 88 PDB declaration: 93-meric（93） Consistent with all polymers |
Chain Am
1–118（118 aa）
|
Not recorded | ZN ZINC ION × 6 MG MAGNESIUM ION × 335 5GP GUANOSINE-5'-MONOPHOSPHATE × 2 SPM SPERMINE × 3 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE-PROPANE
|
Resolution 3.40 Å |
| 7NSI 55S mammalian mitochondrial ribosome with mtRRF (pre) and tRNA(P/E) Deposited 2021-03-07 | Assembly 1 Protein–RNA Heteromer；Protein × 88 PDB declaration: 93-meric（93） Consistent with all polymers |
Chain Am
1–118（118 aa）
|
Not recorded | MG MAGNESIUM ION × 327 ZN ZINC ION × 6 SPM SPERMINE × 3 5GP GUANOSINE-5'-MONOPHOSPHATE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE-PROPANE
|
Resolution 4.60 Å |
| 7NSJ 55S mammalian mitochondrial ribosome with tRNA(P/P) and tRNA(E*) Deposited 2021-03-07 | Assembly 1 Protein–RNA Heteromer；Protein × 87 PDB declaration: 93-meric（93） Consistent with all polymers |
Chain Am
1–118（118 aa）
|
Not recorded | MG MAGNESIUM ION × 324 ZN ZINC ION × 6 SPM SPERMINE × 3 5GP GUANOSINE-5'-MONOPHOSPHATE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE-PROPANE
|
Resolution 3.90 Å |
| 8OIN 55S mammalian mitochondrial ribosome with mtRF1 and P-site tRNA Deposited 2023-03-23 | Assembly 1 Protein–RNA Heteromer；Protein × 88 PDB declaration: 94-meric（94） Consistent with all polymers |
Chain Ac
1–118（118 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | MG MAGNESIUM ION × 332 K POTASSIUM ION × 41 PHE PHENYLALANINE × 1 ZN ZINC ION × 3 FES FE2/S2 (INORGANIC) CLUSTER × 3 SPM SPERMINE × 1 SPD SPERMIDINE × 1 FME N-FORMYLMETHIONINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5；20mM HEPES-KOH pH=7.5, 100mM KCl, 20mM MgCl2, ~1% sucrose (from mtRF1 storage buffer)
cryo-EM vitrification conditions
Cryogen ETHANE-PROPANE
|
Resolution 3.60 Å |
| 8OIP 28S mammalian mitochondrial small ribosomal subunit with mtRF1 and P-site tRNA Deposited 2023-03-23 | Assembly 1 Protein–RNA Heteromer；Protein × 33 PDB declaration: 36-meric（36） Consistent with all polymers |
Chain Ac
1–118（118 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | MG MAGNESIUM ION × 127 SPM SPERMINE × 1 SPD SPERMIDINE × 1 K POTASSIUM ION × 11 ZN ZINC ION × 1 FS2 FE-S-O HYBRID CLUSTER × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5；20mM HEPES-KOH pH=7.5, 100mM KCl, 20mM MgCl2, ~1% sucrose (from mtRF1 storage buffer)
cryo-EM vitrification conditions
Cryogen ETHANE-PROPANE
|
Resolution 3.60 Å |