Current Protein Identity：O27564
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Difference tags compare only the current result set; every original PDB and assembly record remains separate.
Related-Structure Differences
Each row represents one biological assembly in one PDB entry; multiple monomers of the same protein are listed separately.
| PDB Entry | Assembly / Oligomeric State | Construct | Mutations and Modifications | Ligands, Ions and Non-polymers | Experimental Method | Experimental Conditions | Structure Quality |
|---|---|---|---|---|---|---|---|
| 1LNQ CRYSTAL STRUCTURE OF MTHK AT 3.3 A Deposited 2002-05-03 | Assembly 1 Protein homooligomer Homooligomer；Protein × 8 PDB declaration: octameric（8） Consistent with protein count |
Chain A
1–336（336 aa）
Chain B
1–336（336 aa）
Chain C
1–336（336 aa）
Chain D
1–336（336 aa）
Chain E
1–336（336 aa）
Chain F
1–336（336 aa）
Chain G
1–336（336 aa）
Chain H
1–336（336 aa）
|
Not recorded | CA CALCIUM ION × 8 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 6.5；293 K；PEGMME350, CaCl2, MES, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K
|
Resolution 3.30 Å R-free 0.318 |
| 2AEF Crystal Structures of the MthK RCK Domain in Ca2+ bound form Deposited 2005-07-22 | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
107–336（230 aa）
Fragment：isoform Soluble
Chain B
107–336（230 aa）
Fragment：isoform Soluble
|
Not recorded | CA CALCIUM ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 4.5；293 K；PEG 4000, calcium chloride, Ammonium formate, sodium acetate, pH 4.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K
|
Resolution 1.70 Å R-free 0.268 |
| 2AEJ Crystal Structures of the MthK RCK Domain in no Ca2+ bound form Deposited 2005-07-22 | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
107–336（230 aa）
Fragment：isoform Soluble
Chain B
107–336（230 aa）
Fragment：isoform Soluble
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 4.5；293 K；PEG 4000, Ammonium formate, sodium acetate , pH 4.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K
|
Resolution 2.10 Å R-free 0.245 |
| 2AEM Crystal Structures of the MthK RCK Domain Deposited 2005-07-22 | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
107–336（230 aa）
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 5.5；293 K；PEG 400, Ammonium sulfate, cacodylate , pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K
|
Resolution 2.80 Å R-free 0.263 |
| 2FY8 Crystal structure of MthK rck domain in its ligand-free gating-ring form Deposited 2006-02-07 | Assembly 1 Protein homooligomer Homooligomer；Protein × 8 PDB declaration: octameric（8） Consistent with protein count |
Chain A
107–336（230 aa）
Fragment：soluble isoform, residues 107-336
Chain B
107–336（230 aa）
Fragment：soluble isoform, residues 107-336
Chain C
107–336（230 aa）
Fragment：soluble isoform, residues 107-336
Chain D
107–336（230 aa）
Fragment：soluble isoform, residues 107-336
|
Mutation：D184N Mutation：D184N Mutation：D184N Mutation：D184N | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8；293 K；0.6 M Na/K PO4, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K
|
Resolution 2.79 Å R-free 0.254 |
| 2FY8 Crystal structure of MthK rck domain in its ligand-free gating-ring form Deposited 2006-02-07 | Assembly 2 Protein homooligomer Homooligomer；Protein × 8 PDB declaration: octameric（8） Consistent with protein count |
Chain E
107–336（230 aa）
Fragment：soluble isoform, residues 107-336
Chain F
107–336（230 aa）
Fragment：soluble isoform, residues 107-336
Chain G
107–336（230 aa）
Fragment：soluble isoform, residues 107-336
Chain H
107–336（230 aa）
Fragment：soluble isoform, residues 107-336
|
Mutation：D184N Mutation：D184N Mutation：D184N Mutation：D184N | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8；293 K；0.6 M Na/K PO4, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K
|
Resolution 2.79 Å R-free 0.254 |
| 2OGU Crystal structure of the isolated MthK RCK domain Deposited 2007-01-08 | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
107–336（230 aa）
Fragment：RCK domain: Residues 107-336
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；295 K；0.1M Sodium cacodylate pH 6.5, 1.3-1.5M Lithium sulfate, 2% Benzamidine-HCl, VAPOR DIFFUSION, HANGING DROP, temperature 295K
|
Resolution 3.23 Å R-free 0.299 |
| 2OGU Crystal structure of the isolated MthK RCK domain Deposited 2007-01-08 | Assembly 2 Protein homooligomer Homooligomer；Protein × 6 PDB declaration: hexameric（6） Consistent with protein count |
Chain A
107–336（230 aa）
Fragment：RCK domain: Residues 107-336
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；295 K；0.1M Sodium cacodylate pH 6.5, 1.3-1.5M Lithium sulfate, 2% Benzamidine-HCl, VAPOR DIFFUSION, HANGING DROP, temperature 295K
|
Resolution 3.23 Å R-free 0.299 |
| 3KXD Crystal structure of the mthk rck in complex with cadmium Deposited 2009-12-02 | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
116–336（221 aa）
Fragment：RCK DOMAIN
Chain B
116–336（221 aa）
Fragment：RCK DOMAIN
|
Not recorded | CD CADMIUM ION × 4 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 4.7；318 K；1M NH4-FORMATE, 10% PEG 4000, 0.1M NA ACETATE, PH 4.7, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 318K
|
Resolution 2.20 Å R-free 0.254 |
| 3LDC High resolution open MthK pore structure crystallized in 100 mM K+ Deposited 2010-01-12 | Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain A
18–99（82 aa）
Fragment：MthK K+ channel, residues 28-99
|
Mutation：S68H, V77C | K POTASSIUM ION × 20 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.5；293 K；3.0-3.5 M 1,6-Hexandiol, 100 mM HEPES, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K
|
Resolution 1.45 Å R-free 0.202 |
| 3LDD High resolution open MthK pore structure crystallized in 100 mM K+ and further soaked in 99 mM Na+/1 mM K+. Deposited 2010-01-12 | Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain A
18–99（82 aa）
Fragment：MthK K+ channel, residues 28-99
|
Mutation：S68R, V77C | K POTASSIUM ION × 20 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.5；293 K；3.0-3.5 M 1,6-Hexandiol, 100 mM HEPES, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K
|
Resolution 1.45 Å R-free 0.216 |
| 3LDE High resolution open MthK pore structure crystallized in 100 mM K+ and further soaked in 100 mM Na+. Deposited 2010-01-12 | Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain A
18–99（82 aa）
Fragment：MthK K+ channel, residues 28-99
|
Mutation：S68H, V77C | NA SODIUM ION × 16 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.5；293 K；3.0-3.5 M 1,6-Hexandiol, 100 mM HEPES, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K
|
Resolution 2.21 Å R-free 0.208 |
| 3OUS MthK channel pore T59A mutant Deposited 2010-09-15 | Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain A
18–99（82 aa）
|
Mutation：T59A | K POTASSIUM ION × 20 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 6.5；293 K；1M 1,6-hexanediol, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K
|
Resolution 1.75 Å R-free 0.236 |
| 3R65 MthK channel pore E92Q mutant Deposited 2011-03-21 | Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain A
18–99（82 aa）
Fragment：CALCIUM-GATED POTASSIUM CHANNEL MTHK PORE (UNP Residues 18-99)
|
Mutation：E92Q | K POTASSIUM ION × 16 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 6；293 K；3.0-3.5 M 1,6-Hexandiol, 100 mM MES, pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K
|
Resolution 1.80 Å R-free 0.225 |
| 3RBX MthK RCK domain D184N mutant, Ca2+-bound Deposited 2011-03-30 | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
107–336（230 aa）
Fragment：unp residues 107-336
Chain B
107–336（230 aa）
Fragment：unp residues 107-336
|
Mutation：D184N Mutation：D184N | CA CALCIUM ION × 4 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5.7；295 K；20% PEG 3350, 0.1 M CaCl2, 0.1 M MES, VAPOR DIFFUSION, HANGING DROP, temperature 295.0K, pH 5.7
|
Resolution 2.80 Å R-free 0.274 |
| 3RBX MthK RCK domain D184N mutant, Ca2+-bound Deposited 2011-03-30 | Assembly 2 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain C
107–336（230 aa）
Fragment：unp residues 107-336
Chain D
107–336（230 aa）
Fragment：unp residues 107-336
|
Mutation：D184N Mutation：D184N | CA CALCIUM ION × 4 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5.7；295 K；20% PEG 3350, 0.1 M CaCl2, 0.1 M MES, VAPOR DIFFUSION, HANGING DROP, temperature 295.0K, pH 5.7
|
Resolution 2.80 Å R-free 0.274 |
| 3RBX MthK RCK domain D184N mutant, Ca2+-bound Deposited 2011-03-30 | Assembly 3 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain E
107–336（230 aa）
Fragment：unp residues 107-336
Chain F
107–336（230 aa）
Fragment：unp residues 107-336
|
Mutation：D184N Mutation：D184N | CA CALCIUM ION × 4 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5.7；295 K；20% PEG 3350, 0.1 M CaCl2, 0.1 M MES, VAPOR DIFFUSION, HANGING DROP, temperature 295.0K, pH 5.7
|
Resolution 2.80 Å R-free 0.274 |
| 3RBZ MthK channel, Ca2+-bound Deposited 2011-03-30 | Assembly 1 Protein homooligomer Homooligomer；Protein × 8 PDB declaration: octameric（8） Consistent with protein count |
Chain A
1–336（336 aa）
Chain B
1–336（336 aa）
Chain C
1–336（336 aa）
Chain D
1–336（336 aa）
|
Mutation：G85A, E92A, E96T, M107I Mutation：G85A, E92A, E96T, M107I Mutation：G85A, E92A, E96T, M107I Mutation：G85A, E92A, E96T, M107I | CA CALCIUM ION × 24 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；295 K；26% PEG 350-MME, 0.2 M CaCl2, 0.1 M MES, VAPOR DIFFUSION, HANGING DROP, temperature 295K, pH 6.5
|
Resolution 3.40 Å R-free 0.288 |
| 4EI2 Crystal Structures of MthK RCK gating ring bound to Barium Deposited 2012-04-04 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
107–336（230 aa）
Fragment：residues 115-336
|
Not recorded | BA BARIUM ION × 5 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 8.5；295 K；28% polyethylene glycol (PEG) 300, 100 mM BaCl2, 100 mM Tris, pH 8.5, VAPOR DIFFUSION, temperature 295 K
|
Resolution 3.11 Å R-free 0.242 |
| 4EI2 Crystal Structures of MthK RCK gating ring bound to Barium Deposited 2012-04-04 | Assembly 10 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain J
107–336（230 aa）
Fragment：residues 115-336
|
Not recorded | BA BARIUM ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 8.5；295 K；28% polyethylene glycol (PEG) 300, 100 mM BaCl2, 100 mM Tris, pH 8.5, VAPOR DIFFUSION, temperature 295 K
|
Resolution 3.11 Å R-free 0.242 |
| 4EI2 Crystal Structures of MthK RCK gating ring bound to Barium Deposited 2012-04-04 | Assembly 11 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain K
107–336（230 aa）
Fragment：residues 115-336
|
Not recorded | BA BARIUM ION × 4 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 8.5；295 K；28% polyethylene glycol (PEG) 300, 100 mM BaCl2, 100 mM Tris, pH 8.5, VAPOR DIFFUSION, temperature 295 K
|
Resolution 3.11 Å R-free 0.242 |
| 4EI2 Crystal Structures of MthK RCK gating ring bound to Barium Deposited 2012-04-04 | Assembly 12 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain L
107–336（230 aa）
Fragment：residues 115-336
|
Not recorded | BA BARIUM ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 8.5；295 K；28% polyethylene glycol (PEG) 300, 100 mM BaCl2, 100 mM Tris, pH 8.5, VAPOR DIFFUSION, temperature 295 K
|
Resolution 3.11 Å R-free 0.242 |
| 4EI2 Crystal Structures of MthK RCK gating ring bound to Barium Deposited 2012-04-04 | Assembly 13 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain M
107–336（230 aa）
Fragment：residues 115-336
|
Not recorded | BA BARIUM ION × 4 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 8.5；295 K；28% polyethylene glycol (PEG) 300, 100 mM BaCl2, 100 mM Tris, pH 8.5, VAPOR DIFFUSION, temperature 295 K
|
Resolution 3.11 Å R-free 0.242 |
| 4EI2 Crystal Structures of MthK RCK gating ring bound to Barium Deposited 2012-04-04 | Assembly 14 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain N
107–336（230 aa）
Fragment：residues 115-336
|
Not recorded | BA BARIUM ION × 3 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 8.5；295 K；28% polyethylene glycol (PEG) 300, 100 mM BaCl2, 100 mM Tris, pH 8.5, VAPOR DIFFUSION, temperature 295 K
|
Resolution 3.11 Å R-free 0.242 |
| 4EI2 Crystal Structures of MthK RCK gating ring bound to Barium Deposited 2012-04-04 | Assembly 15 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain O
107–336（230 aa）
Fragment：residues 115-336
|
Not recorded | BA BARIUM ION × 3 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 8.5；295 K；28% polyethylene glycol (PEG) 300, 100 mM BaCl2, 100 mM Tris, pH 8.5, VAPOR DIFFUSION, temperature 295 K
|
Resolution 3.11 Å R-free 0.242 |
| 4EI2 Crystal Structures of MthK RCK gating ring bound to Barium Deposited 2012-04-04 | Assembly 16 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain P
107–336（230 aa）
Fragment：residues 115-336
|
Not recorded | BA BARIUM ION × 3 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 8.5；295 K；28% polyethylene glycol (PEG) 300, 100 mM BaCl2, 100 mM Tris, pH 8.5, VAPOR DIFFUSION, temperature 295 K
|
Resolution 3.11 Å R-free 0.242 |
| 4EI2 Crystal Structures of MthK RCK gating ring bound to Barium Deposited 2012-04-04 | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain B
107–336（230 aa）
Fragment：residues 115-336
|
Not recorded | BA BARIUM ION × 4 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 8.5；295 K；28% polyethylene glycol (PEG) 300, 100 mM BaCl2, 100 mM Tris, pH 8.5, VAPOR DIFFUSION, temperature 295 K
|
Resolution 3.11 Å R-free 0.242 |
| 4EI2 Crystal Structures of MthK RCK gating ring bound to Barium Deposited 2012-04-04 | Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain C
107–336（230 aa）
Fragment：residues 115-336
|
Not recorded | BA BARIUM ION × 3 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 8.5；295 K；28% polyethylene glycol (PEG) 300, 100 mM BaCl2, 100 mM Tris, pH 8.5, VAPOR DIFFUSION, temperature 295 K
|
Resolution 3.11 Å R-free 0.242 |
| 4EI2 Crystal Structures of MthK RCK gating ring bound to Barium Deposited 2012-04-04 | Assembly 4 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain D
107–336（230 aa）
Fragment：residues 115-336
|
Not recorded | BA BARIUM ION × 5 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 8.5；295 K；28% polyethylene glycol (PEG) 300, 100 mM BaCl2, 100 mM Tris, pH 8.5, VAPOR DIFFUSION, temperature 295 K
|
Resolution 3.11 Å R-free 0.242 |
| 4EI2 Crystal Structures of MthK RCK gating ring bound to Barium Deposited 2012-04-04 | Assembly 5 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain E
107–336（230 aa）
Fragment：residues 115-336
|
Not recorded | BA BARIUM ION × 6 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 8.5；295 K；28% polyethylene glycol (PEG) 300, 100 mM BaCl2, 100 mM Tris, pH 8.5, VAPOR DIFFUSION, temperature 295 K
|
Resolution 3.11 Å R-free 0.242 |
| 4EI2 Crystal Structures of MthK RCK gating ring bound to Barium Deposited 2012-04-04 | Assembly 6 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain F
107–336（230 aa）
Fragment：residues 115-336
|
Not recorded | BA BARIUM ION × 4 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 8.5；295 K；28% polyethylene glycol (PEG) 300, 100 mM BaCl2, 100 mM Tris, pH 8.5, VAPOR DIFFUSION, temperature 295 K
|
Resolution 3.11 Å R-free 0.242 |
| 4EI2 Crystal Structures of MthK RCK gating ring bound to Barium Deposited 2012-04-04 | Assembly 7 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain G
107–336（230 aa）
Fragment：residues 115-336
|
Not recorded | BA BARIUM ION × 4 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 8.5；295 K；28% polyethylene glycol (PEG) 300, 100 mM BaCl2, 100 mM Tris, pH 8.5, VAPOR DIFFUSION, temperature 295 K
|
Resolution 3.11 Å R-free 0.242 |
| 4EI2 Crystal Structures of MthK RCK gating ring bound to Barium Deposited 2012-04-04 | Assembly 8 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain H
107–336（230 aa）
Fragment：residues 115-336
|
Not recorded | BA BARIUM ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 8.5；295 K；28% polyethylene glycol (PEG) 300, 100 mM BaCl2, 100 mM Tris, pH 8.5, VAPOR DIFFUSION, temperature 295 K
|
Resolution 3.11 Å R-free 0.242 |
| 4EI2 Crystal Structures of MthK RCK gating ring bound to Barium Deposited 2012-04-04 | Assembly 9 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain I
107–336（230 aa）
Fragment：residues 115-336
|
Not recorded | BA BARIUM ION × 3 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 8.5；295 K；28% polyethylene glycol (PEG) 300, 100 mM BaCl2, 100 mM Tris, pH 8.5, VAPOR DIFFUSION, temperature 295 K
|
Resolution 3.11 Å R-free 0.242 |
| 4HYO Crystal Structure of MthK Pore Deposited 2012-11-13 | Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain A
11–101（91 aa）
Fragment：unp residues 11-101
Chain B
11–101（91 aa）
Fragment：unp residues 11-101
Chain C
11–101（91 aa）
Fragment：unp residues 11-101
Chain D
11–101（91 aa）
Fragment：unp residues 11-101
|
Mutation：S68H, V77C Mutation：S68H, V77C Mutation：S68H, V77C Mutation：S68H, V77C | K POTASSIUM ION × 4 HEZ HEXANE-1,6-DIOL × 8 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 6.5；293 K；3.5-4.0M 1,6-hexanediol, 100mM MES , pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K
|
Resolution 1.65 Å R-free 0.181 |
| 4HYO Crystal Structure of MthK Pore Deposited 2012-11-13 | Assembly 2 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain E
11–101（91 aa）
Fragment：unp residues 11-101
Chain F
11–101（91 aa）
Fragment：unp residues 11-101
Chain G
11–101（91 aa）
Fragment：unp residues 11-101
Chain H
11–101（91 aa）
Fragment：unp residues 11-101
|
Mutation：S68H, V77C Mutation：S68H, V77C Mutation：S68H, V77C Mutation：S68H, V77C | K POTASSIUM ION × 4 HEZ HEXANE-1,6-DIOL × 4 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 6.5；293 K；3.5-4.0M 1,6-hexanediol, 100mM MES , pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K
|
Resolution 1.65 Å R-free 0.181 |
| 4HZ3 MthK pore crystallized in presence of TBSb Deposited 2012-11-14 | Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain A
11–101（91 aa）
Fragment：unp residues 11-101
Chain B
11–101（91 aa）
Fragment：unp residues 11-101
Chain C
11–101（91 aa）
Fragment：unp residues 11-101
Chain D
11–101（91 aa）
Fragment：unp residues 11-101
|
Mutation：S68H, V77C Mutation：S68H, V77C Mutation：S68H, V77C Mutation：S68H, V77C | K POTASSIUM ION × 4 HEZ HEXANE-1,6-DIOL × 12 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 6.5；293 K；3.5 4.0M 1,6-hexanediol, 100mM MES, 10mM TBSb , pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K
|
Resolution 1.70 Å R-free 0.192 |
| 4HZ3 MthK pore crystallized in presence of TBSb Deposited 2012-11-14 | Assembly 2 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain E
11–101（91 aa）
Fragment：unp residues 11-101
Chain F
11–101（91 aa）
Fragment：unp residues 11-101
Chain G
11–101（91 aa）
Fragment：unp residues 11-101
Chain H
11–101（91 aa）
Fragment：unp residues 11-101
|
Mutation：S68H, V77C Mutation：S68H, V77C Mutation：S68H, V77C Mutation：S68H, V77C | K POTASSIUM ION × 4 HEZ HEXANE-1,6-DIOL × 8 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 6.5；293 K；3.5 4.0M 1,6-hexanediol, 100mM MES, 10mM TBSb , pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K
|
Resolution 1.70 Å R-free 0.192 |
| 4L73 Ca2+-bound MthK RCK domain at 2.5 Angstrom Deposited 2013-06-13 | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
107–336（230 aa）
Fragment：RCK domain (UNP residues 107-336)
Chain B
107–336（230 aa）
Fragment：RCK domain (UNP residues 107-336)
|
Not recorded | CA CALCIUM ION × 5 NA SODIUM ION × 8 CL CHLORIDE ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 4.8；295 K；12% PEG4000, 1 M ammonium formate, 0.1 M sodium acetate, pH 4.8, 1 M calcium chloride, VAPOR DIFFUSION, HANGING DROP, temperature 295K
|
Resolution 2.50 Å R-free 0.226 |
| 4L74 Ca2+-bound MthK RCK domain at 1.9 Angstrom with single ligand Deposited 2013-06-13 | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
107–336（230 aa）
Fragment：RCK domain (UNP residues 107-336)
Chain B
107–336（230 aa）
Fragment：RCK domain (UNP residues 107-336)
|
Not recorded | CA CALCIUM ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 4.8；295 K；6% PEG4000, 1 M ammonium formate, 0.1 M sodium acetate, pH 4.8, 0.2 M calcium chloride, VAPOR DIFFUSION, HANGING DROP, temperature 295K
|
Resolution 1.84 Å R-free 0.253 |
| 4L75 Ca2+-bound D184N mutant MthK RCK domain at 2.4 Angstrom Deposited 2013-06-13 | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
107–336（230 aa）
Fragment：RCK domain (UNP residues 107-336)
Chain B
107–336（230 aa）
Fragment：RCK domain (UNP residues 107-336)
|
Mutation：D184N Mutation：D184N | CA CALCIUM ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 4.8；295 K；7% PEG4000, 1 M ammonium formate, 0.1 M sodium acetate, pH 4.8, 0.3 M calcium chloride, VAPOR DIFFUSION, HANGING DROP, temperature 295K
|
Resolution 2.39 Å R-free 0.245 |
| 4L75 Ca2+-bound D184N mutant MthK RCK domain at 2.4 Angstrom Deposited 2013-06-13 | Assembly 2 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain C
107–336（230 aa）
Fragment：RCK domain (UNP residues 107-336)
Chain D
107–336（230 aa）
Fragment：RCK domain (UNP residues 107-336)
|
Mutation：D184N Mutation：D184N | CA CALCIUM ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 4.8；295 K；7% PEG4000, 1 M ammonium formate, 0.1 M sodium acetate, pH 4.8, 0.3 M calcium chloride, VAPOR DIFFUSION, HANGING DROP, temperature 295K
|
Resolution 2.39 Å R-free 0.245 |
| 4L75 Ca2+-bound D184N mutant MthK RCK domain at 2.4 Angstrom Deposited 2013-06-13 | Assembly 3 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain E
107–336（230 aa）
Fragment：RCK domain (UNP residues 107-336)
Chain F
107–336（230 aa）
Fragment：RCK domain (UNP residues 107-336)
|
Mutation：D184N Mutation：D184N | CA CALCIUM ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 4.8；295 K；7% PEG4000, 1 M ammonium formate, 0.1 M sodium acetate, pH 4.8, 0.3 M calcium chloride, VAPOR DIFFUSION, HANGING DROP, temperature 295K
|
Resolution 2.39 Å R-free 0.245 |
| 4L76 Ca2+-bound E212Q mutant MthK RCK domain Deposited 2013-06-13 | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
107–336（230 aa）
Fragment：RCK domain (UNP residues 107-336)
Chain B
107–336（230 aa）
Fragment：RCK domain (UNP residues 107-336)
|
Mutation：E212Q Mutation：E212Q | CA CALCIUM ION × 4 NA SODIUM ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 4.8；295 K；8% PEG4000, 1 M ammonium formate, 0.1 M sodium acetate, pH 4.8, 0.2 M calcium chloride, VAPOR DIFFUSION, HANGING DROP, temperature 295K
|
Resolution 2.99 Å R-free 0.263 |
| 4L76 Ca2+-bound E212Q mutant MthK RCK domain Deposited 2013-06-13 | Assembly 2 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain C
107–336（230 aa）
Fragment：RCK domain (UNP residues 107-336)
Chain D
107–336（230 aa）
Fragment：RCK domain (UNP residues 107-336)
|
Mutation：E212Q Mutation：E212Q | CA CALCIUM ION × 5 NA SODIUM ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 4.8；295 K；8% PEG4000, 1 M ammonium formate, 0.1 M sodium acetate, pH 4.8, 0.2 M calcium chloride, VAPOR DIFFUSION, HANGING DROP, temperature 295K
|
Resolution 2.99 Å R-free 0.263 |
| 4L76 Ca2+-bound E212Q mutant MthK RCK domain Deposited 2013-06-13 | Assembly 3 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain E
107–336（230 aa）
Fragment：RCK domain (UNP residues 107-336)
Chain F
107–336（230 aa）
Fragment：RCK domain (UNP residues 107-336)
|
Mutation：E212Q Mutation：E212Q | CA CALCIUM ION × 4 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 4.8；295 K；8% PEG4000, 1 M ammonium formate, 0.1 M sodium acetate, pH 4.8, 0.2 M calcium chloride, VAPOR DIFFUSION, HANGING DROP, temperature 295K
|
Resolution 2.99 Å R-free 0.263 |
| 4QE7 Open MthK pore structure soaked in 10 mM Ba2+/100 mM Na+ Deposited 2014-05-15 | Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain A
19–99（81 aa）
Fragment：UNP residues 19-99
|
Mutation：S68H, V77C | BA BARIUM ION × 12 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.5；298 K；3.0-3.5M 1,6-Hexandiol, 100mM Hepes, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K
|
Resolution 2.40 Å R-free 0.265 |
| 4QE9 Open MthK pore structure soaked in 10 mM Ba2+/100 mM K+ Deposited 2014-05-15 | Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain A
18–100（83 aa）
Fragment：UNP residues 18-100
|
Mutation：S51H, V60C | K POTASSIUM ION × 24 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.5；298 K；3.0-3.5M 1,6-Hexandiol, 100mM HEPES, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K
|
Resolution 2.15 Å R-free 0.257 |
| 4RO0 Crystal structure of MthK gating ring in a ligand-free form Deposited 2014-10-27 | Assembly 1 Protein homooligomer Homooligomer；Protein × 8 PDB declaration: octameric（8） Consistent with protein count |
Chain A
107–336（230 aa）
Fragment：MthK RCK, UNP residues 107-336
Chain B
107–336（230 aa）
Fragment：MthK RCK, UNP residues 107-336
Chain C
107–336（230 aa）
Fragment：MthK RCK, UNP residues 107-336
Chain D
107–336（230 aa）
Fragment：MthK RCK, UNP residues 107-336
Chain a
107–336（230 aa）
Fragment：MthK RCK, UNP residues 107-336
Chain b
107–336（230 aa）
Fragment：MthK RCK, UNP residues 107-336
Chain c
107–336（230 aa）
Fragment：MthK RCK, UNP residues 107-336
Chain d
107–336（230 aa）
Fragment：MthK RCK, UNP residues 107-336
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；0.4 M KCl, 0.1 M Tris and 20-25% PEGMME 350, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K
|
Resolution 3.18 Å R-free 0.301 |
| 4RO0 Crystal structure of MthK gating ring in a ligand-free form Deposited 2014-10-27 | Assembly 2 Protein homooligomer Homooligomer；Protein × 8 PDB declaration: octameric（8） Consistent with protein count |
Chain E
107–336（230 aa）
Fragment：MthK RCK, UNP residues 107-336
Chain F
107–336（230 aa）
Fragment：MthK RCK, UNP residues 107-336
Chain G
107–336（230 aa）
Fragment：MthK RCK, UNP residues 107-336
Chain H
107–336（230 aa）
Fragment：MthK RCK, UNP residues 107-336
Chain e
107–336（230 aa）
Fragment：MthK RCK, UNP residues 107-336
Chain f
107–336（230 aa）
Fragment：MthK RCK, UNP residues 107-336
Chain g
107–336（230 aa）
Fragment：MthK RCK, UNP residues 107-336
Chain h
107–336（230 aa）
Fragment：MthK RCK, UNP residues 107-336
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；0.4 M KCl, 0.1 M Tris and 20-25% PEGMME 350, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K
|
Resolution 3.18 Å R-free 0.301 |
| 4RO0 Crystal structure of MthK gating ring in a ligand-free form Deposited 2014-10-27 | Assembly 3 Protein homooligomer Homooligomer；Protein × 8 PDB declaration: octameric（8） Consistent with protein count |
Chain I
107–336（230 aa）
Fragment：MthK RCK, UNP residues 107-336
Chain J
107–336（230 aa）
Fragment：MthK RCK, UNP residues 107-336
Chain K
107–336（230 aa）
Fragment：MthK RCK, UNP residues 107-336
Chain L
107–336（230 aa）
Fragment：MthK RCK, UNP residues 107-336
Chain i
107–336（230 aa）
Fragment：MthK RCK, UNP residues 107-336
Chain j
107–336（230 aa）
Fragment：MthK RCK, UNP residues 107-336
Chain k
107–336（230 aa）
Fragment：MthK RCK, UNP residues 107-336
Chain l
107–336（230 aa）
Fragment：MthK RCK, UNP residues 107-336
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；0.4 M KCl, 0.1 M Tris and 20-25% PEGMME 350, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K
|
Resolution 3.18 Å R-free 0.301 |
| 4RO0 Crystal structure of MthK gating ring in a ligand-free form Deposited 2014-10-27 | Assembly 4 Protein homooligomer Homooligomer；Protein × 8 PDB declaration: octameric（8） Consistent with protein count |
Chain M
107–336（230 aa）
Fragment：MthK RCK, UNP residues 107-336
Chain N
107–336（230 aa）
Fragment：MthK RCK, UNP residues 107-336
Chain O
107–336（230 aa）
Fragment：MthK RCK, UNP residues 107-336
Chain P
107–336（230 aa）
Fragment：MthK RCK, UNP residues 107-336
Chain m
107–336（230 aa）
Fragment：MthK RCK, UNP residues 107-336
Chain n
107–336（230 aa）
Fragment：MthK RCK, UNP residues 107-336
Chain o
107–336（230 aa）
Fragment：MthK RCK, UNP residues 107-336
Chain p
107–336（230 aa）
Fragment：MthK RCK, UNP residues 107-336
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；0.4 M KCl, 0.1 M Tris and 20-25% PEGMME 350, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K
|
Resolution 3.18 Å R-free 0.301 |
| 4RO0 Crystal structure of MthK gating ring in a ligand-free form Deposited 2014-10-27 | Assembly 5 Protein homooligomer Homooligomer；Protein × 8 PDB declaration: octameric（8） Consistent with protein count |
Chain Q
107–336（230 aa）
Fragment：MthK RCK, UNP residues 107-336
Chain R
107–336（230 aa）
Fragment：MthK RCK, UNP residues 107-336
Chain S
107–336（230 aa）
Fragment：MthK RCK, UNP residues 107-336
Chain T
107–336（230 aa）
Fragment：MthK RCK, UNP residues 107-336
Chain q
107–336（230 aa）
Fragment：MthK RCK, UNP residues 107-336
Chain r
107–336（230 aa）
Fragment：MthK RCK, UNP residues 107-336
Chain s
107–336（230 aa）
Fragment：MthK RCK, UNP residues 107-336
Chain t
107–336（230 aa）
Fragment：MthK RCK, UNP residues 107-336
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；0.4 M KCl, 0.1 M Tris and 20-25% PEGMME 350, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K
|
Resolution 3.18 Å R-free 0.301 |
| 5BKI Blocker-free closed MthK channel in nanodisc Deposited 2021-03-19 | Assembly 1 Protein homooligomer Homooligomer；Protein × 8 PDB declaration: octameric（8） Consistent with protein count |
Chain A
1–336（336 aa）
Chain B
1–336（336 aa）
Chain C
1–336（336 aa）
Chain D
1–336（336 aa）
Chain E
1–336（336 aa）
Chain F
1–336（336 aa）
Chain G
1–336（336 aa）
Chain H
1–336（336 aa）
|
Not recorded | CD CADMIUM ION × 12 PGW (1R)-2-{[(S)-{[(2S)-2,3-dihydroxypropyl]oxy}(hydroxy)phosphoryl]oxy}-1-[(hexadecanoyloxy)methyl]ethyl (9Z)-octadec-9-enoate × 4 K POTASSIUM ION × 5 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 8.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.10 Å |
| 5BKJ TPeA-bound closed MthK channel in nanodisc Deposited 2021-03-19 | Assembly 1 Protein homooligomer Homooligomer；Protein × 8 PDB declaration: octameric（8） Consistent with protein count |
Chain A
1–336（336 aa）
Chain B
1–336（336 aa）
Chain C
1–336（336 aa）
Chain D
1–336（336 aa）
Chain E
1–336（336 aa）
Chain F
1–336（336 aa）
Chain G
1–336（336 aa）
Chain H
1–336（336 aa）
|
Not recorded | PGW (1R)-2-{[(S)-{[(2S)-2,3-dihydroxypropyl]oxy}(hydroxy)phosphoryl]oxy}-1-[(hexadecanoyloxy)methyl]ethyl (9Z)-octadec-9-enoate × 4 K POTASSIUM ION × 2 YQ1 1-(tripentyl-$l^{4}-azanyl)pentane × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 8.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.50 Å |
| 5BKK bbTBA-bound closed MthK channel in nanodisc Deposited 2021-03-19 | Assembly 1 Protein homooligomer Homooligomer；Protein × 8 PDB declaration: octameric（8） Consistent with protein count |
Chain A
1–336（336 aa）
Chain B
1–336（336 aa）
Chain C
1–336（336 aa）
Chain D
1–336（336 aa）
Chain E
1–336（336 aa）
Chain F
1–336（336 aa）
Chain G
1–336（336 aa）
Chain H
1–336（336 aa）
|
Not recorded | PGW (1R)-2-{[(S)-{[(2S)-2,3-dihydroxypropyl]oxy}(hydroxy)phosphoryl]oxy}-1-[(hexadecanoyloxy)methyl]ethyl (9Z)-octadec-9-enoate × 2 K POTASSIUM ION × 3 YQ4 (~{R})-phenyl-[4-[(tributyl-$l^{4}-azanyl)methyl]phenyl]methanol × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 8.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.50 Å |
| 6OLY Full-length MthK channel at 3.1 angstrom resolution Deposited 2019-04-17 | Assembly 1 Protein homooligomer Homooligomer；Protein × 8 PDB declaration: octameric（8） Consistent with protein count |
Chain A
1–336（336 aa）
Chain B
1–336（336 aa）
Chain C
1–336（336 aa）
Chain D
1–336（336 aa）
|
Mutation：M107I Mutation：M107I Mutation：M107I Mutation：M107I | CA CALCIUM ION × 24 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；300 K；28% PEG350-MME, 0.1M MES pH 6.5, 0.2 M CaCl2
|
Resolution 3.11 Å R-free 0.275 |
| 6U5N Calcium-bound MthK gating ring state 2 Deposited 2019-08-28 | Assembly 1 Protein homooligomer Homooligomer；Protein × 8 PDB declaration: octameric（8） Consistent with protein count |
Chain A
1–336（336 aa）
Chain B
1–336（336 aa）
Chain C
1–336（336 aa）
Chain D
1–336（336 aa）
Chain E
1–336（336 aa）
Chain F
1–336（336 aa）
Chain G
1–336（336 aa）
Chain H
1–336（336 aa）
|
Not recorded | CA CALCIUM ION × 24 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 8.5
cryo-EM vitrification conditions
Cryogen ETHANE；blotting for 2 seconds (blot force 0)
|
Resolution 3.20 Å |
| 6U5P Calcium-bound MthK gating ring state 1 Deposited 2019-08-28 | Assembly 1 Protein homooligomer Homooligomer；Protein × 8 PDB declaration: octameric（8） Consistent with protein count |
Chain A
1–336（336 aa）
Chain B
1–336（336 aa）
Chain C
1–336（336 aa）
Chain D
1–336（336 aa）
Chain E
1–336（336 aa）
Chain F
1–336（336 aa）
Chain G
1–336（336 aa）
Chain H
1–336（336 aa）
|
Not recorded | CA CALCIUM ION × 24 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 8.5
cryo-EM vitrification conditions
Cryogen ETHANE；blotting for 2 seconds (blot force 0)
|
Resolution 3.30 Å |
| 6U5R Calcium-bound MthK closed state Deposited 2019-08-28 | Assembly 1 Protein homooligomer Homooligomer；Protein × 8 PDB declaration: octameric（8） Consistent with protein count |
Chain A
1–336（336 aa）
Chain B
1–336（336 aa）
Chain C
1–336（336 aa）
Chain D
1–336（336 aa）
Chain E
1–336（336 aa）
Chain F
1–336（336 aa）
Chain G
1–336（336 aa）
Chain H
1–336（336 aa）
|
Not recorded | CA CALCIUM ION × 8 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 8.5
cryo-EM vitrification conditions
Cryogen ETHANE；blotting for 2 seconds (blot force 0)
|
Resolution 3.60 Å |
| 6U68 Calcium-bound MthK open-inactivated state 1 Deposited 2019-08-29 | Assembly 1 Protein homooligomer Homooligomer；Protein × 8 PDB declaration: octameric（8） Consistent with protein count |
Chain A
1–336（336 aa）
Chain B
1–336（336 aa）
Chain C
1–336（336 aa）
Chain D
1–336（336 aa）
Chain E
1–336（336 aa）
Chain F
1–336（336 aa）
Chain G
1–336（336 aa）
Chain H
1–336（336 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 8.5
cryo-EM vitrification conditions
Cryogen ETHANE；blotting for 2 s (blot force 0)
|
Resolution 4.50 Å |
| 6U6D MthK closed state with EDTA Deposited 2019-08-29 | Assembly 1 Protein homooligomer Homooligomer；Protein × 8 PDB declaration: octameric（8） Consistent with protein count |
Chain A
1–336（336 aa）
Chain B
1–336（336 aa）
Chain C
1–336（336 aa）
Chain D
1–336（336 aa）
Chain E
1–336（336 aa）
Chain F
1–336（336 aa）
Chain G
1–336（336 aa）
Chain H
1–336（336 aa）
|
Not recorded | K POTASSIUM ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE；blotting for 2 s (blot force 0)
|
Resolution 3.60 Å |
| 6U6E Calcium-bound MthK open-inactivated state 2 Deposited 2019-08-29 | Assembly 1 Protein homooligomer Homooligomer；Protein × 8 PDB declaration: octameric（8） Consistent with protein count |
Chain A
1–336（336 aa）
Chain B
1–336（336 aa）
Chain C
1–336（336 aa）
Chain D
1–336（336 aa）
Chain E
1–336（336 aa）
Chain F
1–336（336 aa）
Chain G
1–336（336 aa）
Chain H
1–336（336 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 8.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 6.30 Å |
| 6U6H Calcium-bound MthK open-inactivated state 3 Deposited 2019-08-29 | Assembly 1 Protein homooligomer Homooligomer；Protein × 8 PDB declaration: octameric（8） Consistent with protein count |
Chain A
1–336（336 aa）
Chain B
1–336（336 aa）
Chain C
1–336（336 aa）
Chain D
1–336（336 aa）
Chain E
1–336（336 aa）
Chain F
1–336（336 aa）
Chain G
1–336（336 aa）
Chain H
1–336（336 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 8.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 5.00 Å |
| 6U9P Wild-type MthK pore in ~150 mM K+ Deposited 2019-09-09 | Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain A
18–99（82 aa）
Fragment：UNP residues 18-99
|
Not recorded | K POTASSIUM ION × 20 HEZ HEXANE-1,6-DIOL × 4 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8；293 K；1:1 protein (in 100 mM potassium chloride, 10 mM MOPS-KOH, pH 8.0) + 3.5-4.0 M 1,6-hexanediol, 100 mM MES-KOH, pH 6.75
|
Resolution 1.65 Å R-free 0.207 |
| 6U9T Wild-type MthK pore in 50 mM K+ Deposited 2019-09-09 | Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain A
18–99（82 aa）
Fragment：UNP residues 18-99
|
Not recorded | K POTASSIUM ION × 20 HEZ HEXANE-1,6-DIOL × 4 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8；293 K；1:1 protein (in 100 mM sodium chloride, 1 mM potassium chloride, 10 mM MOPS, pH 8.0) + 3.5-4.0 M 1,6-hexanediol, 100 mM MES-NaOH, pH 6.75
|
Resolution 1.55 Å R-free 0.210 |
| 6U9Y Wild-type MthK pore in 11 mM K+ Deposited 2019-09-09 | Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain A
18–99（82 aa）
Fragment：UNP residues 18-99
|
Mutation：UNP residues 18-99 | K POTASSIUM ION × 20 HEZ HEXANE-1,6-DIOL × 4 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8；293 K；1:1 protein (in 100 mM sodium chloride, 1 mM potassium chloride, 10 mM MOPS, pH 8.0) + 3.5-4.0 M 1,6-hexanediol, 100 mM MES-NaOH, 10 mM potassium chloride
|
Resolution 1.80 Å R-free 0.217 |
| 6U9Z Wild-type MthK pore in 6 mM K+ Deposited 2019-09-09 | Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain A
18–99（82 aa）
Fragment：UNP residues 18-99
|
Not recorded | K POTASSIUM ION × 20 HEZ HEXANE-1,6-DIOL × 4 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8；293 K；protein (in 100 mM sodium chloride, 1 mM potassium chloride, 10 mM MOPS) + 3.5-4.0 M 1,6-hexanediol, 100 mM MES-NaOH, pH 6.75, 5 mM potassium chloride
|
Resolution 1.95 Å R-free 0.226 |
| 6UWN MthK N-terminal truncation RCK domain state 1 bound with calcium Deposited 2019-11-05 | Assembly 1 Protein homooligomer Homooligomer；Protein × 8 PDB declaration: octameric（8） Consistent with protein count |
Chain A
1–336（336 aa）
Chain B
1–336（336 aa）
Chain C
1–336（336 aa）
Chain D
1–336（336 aa）
Chain E
1–336（336 aa）
Chain F
1–336（336 aa）
Chain G
1–336（336 aa）
Chain H
1–336（336 aa）
|
Not recorded | CA CALCIUM ION × 24 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 8.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.50 Å |
| 6UX4 MthK N-terminal truncation RCK domain state 2 bound with calcium Deposited 2019-11-06 | Assembly 1 Protein homooligomer Homooligomer；Protein × 8 PDB declaration: octameric（8） Consistent with protein count |
Chain A
1–336（336 aa）
Chain B
1–336（336 aa）
Chain C
1–336（336 aa）
Chain D
1–336（336 aa）
Chain E
1–336（336 aa）
Chain F
1–336（336 aa）
Chain G
1–336（336 aa）
Chain H
1–336（336 aa）
|
Not recorded | CA CALCIUM ION × 24 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 8.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.50 Å |
| 6UX7 MthK N-terminal truncation state 1 bound with calcium Deposited 2019-11-06 | Assembly 1 Protein homooligomer Homooligomer；Protein × 8 PDB declaration: octameric（8） Consistent with protein count |
Chain A
1–336（336 aa）
Chain B
1–336（336 aa）
Chain C
1–336（336 aa）
Chain D
1–336（336 aa）
Chain E
1–336（336 aa）
Chain F
1–336（336 aa）
Chain G
1–336（336 aa）
Chain H
1–336（336 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 8.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 6.70 Å |
| 6UXA MthK N-terminal truncation state 2 bound with calcium Deposited 2019-11-07 | Assembly 1 Protein homooligomer Homooligomer；Protein × 8 PDB declaration: octameric（8） Consistent with protein count |
Chain A
1–336（336 aa）
Chain B
1–336（336 aa）
Chain C
1–336（336 aa）
Chain D
1–336（336 aa）
Chain E
1–336（336 aa）
Chain F
1–336（336 aa）
Chain G
1–336（336 aa）
Chain H
1–336（336 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 8.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.50 Å |
| 6UXB MthK N-terminal truncation state 3 bound with calcium Deposited 2019-11-07 | Assembly 1 Protein homooligomer Homooligomer；Protein × 8 PDB declaration: octameric（8） Consistent with protein count |
Chain A
1–336（336 aa）
Chain B
1–336（336 aa）
Chain C
1–336（336 aa）
Chain D
1–336（336 aa）
Chain E
1–336（336 aa）
Chain F
1–336（336 aa）
Chain G
1–336（336 aa）
Chain H
1–336（336 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 8.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.90 Å |
| 8DJB MthK-A90L mutant in closed state with 0 Ca2+ Deposited 2022-06-30 | Assembly 1 Protein homooligomer Homooligomer；Protein × 8 PDB declaration: octameric（8） Consistent with protein count |
Chain A
1–336（336 aa）
Chain B
1–336（336 aa）
Chain C
1–336（336 aa）
Chain D
1–336（336 aa）
Chain E
1–336（336 aa）
Chain F
1–336（336 aa）
Chain G
1–336（336 aa）
Chain H
1–336（336 aa）
|
Not recorded | K POTASSIUM ION × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 8.5
cryo-EM vitrification conditions
Cryogen ETHANE；BLOTTING FOR 2 S (BLOT FORCE 1)
|
Resolution 3.18 Å |
| 8FZ7 TpeA bound closed MthK-A88F mutant in nanodisc Deposited 2023-01-27 | Assembly 1 Protein homooligomer Homooligomer；Protein × 8 PDB declaration: octameric（8） Consistent with protein count |
Chain A
1–336（336 aa）
Chain B
1–336（336 aa）
Chain C
1–336（336 aa）
Chain D
1–336（336 aa）
Chain E
1–336（336 aa）
Chain F
1–336（336 aa）
Chain G
1–336（336 aa）
Chain H
1–336（336 aa）
|
Not recorded | PGW (1R)-2-{[(S)-{[(2S)-2,3-dihydroxypropyl]oxy}(hydroxy)phosphoryl]oxy}-1-[(hexadecanoyloxy)methyl]ethyl (9Z)-octadec-9-enoate × 4 YQ1 1-(tripentyl-$l^{4}-azanyl)pentane × 1 K POTASSIUM ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 8.5
cryo-EM vitrification conditions
Cryogen ETHANE；BLOTTING FOR 2 S (BLOT FORCE 0)
|
Resolution 2.88 Å |
| 9Z2M Ca2+-bound MthK WT in lipid nanodiscs composed of 14:1PC (75%) and POPG (25%) Deposited 2025-11-05 | Assembly 1 Protein homooligomer Homooligomer；Protein × 8 PDB declaration: octameric（8） Consistent with protein count |
Chain A
1–336（336 aa）
Chain B
1–336（336 aa）
Chain C
1–336（336 aa）
Chain D
1–336（336 aa）
Chain E
1–336（336 aa）
Chain F
1–336（336 aa）
Chain G
1–336（336 aa）
Chain H
1–336（336 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 8.5；20 mM HEPES-KOH pH 8.5
100 mM KCl
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.40 Å |
| 9Z2V Ca2+-bound MthK WT in lipid nanodiscs composed of 22:1PC (75%) and POPG (25%) Deposited 2025-11-05 | Assembly 1 Protein homooligomer Homooligomer；Protein × 8 PDB declaration: octameric（8） Consistent with protein count |
Chain A
1–336（336 aa）
Chain B
1–336（336 aa）
Chain C
1–336（336 aa）
Chain D
1–336（336 aa）
Chain E
1–336（336 aa）
Chain F
1–336（336 aa）
Chain G
1–336（336 aa）
Chain H
1–336（336 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 8.5；20 mM HEPES-KOH pH 8.5
100 mM KCl
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.80 Å |