Current Protein Identity：P02640
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Difference tags compare only the current result set; every original PDB and assembly record remains separate.
Related-Structure Differences
Each row represents one biological assembly in one PDB entry; multiple monomers of the same protein are listed separately.
| PDB Entry | Assembly / Oligomeric State | Construct | Mutations and Modifications | Ligands, Ions and Non-polymers | Experimental Method | Experimental Conditions | Structure Quality |
|---|---|---|---|---|---|---|---|
| 1QQV SOLUTION STRUCTURE OF THE HEADPIECE DOMAIN OF CHICKEN VILLIN Deposited 1999-06-08 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
760–826（67 aa）
Fragment：HP67
|
Not recorded | No recorded non-water small molecule | SOLUTION NMR |
NMR measurement conditions
pH 7；293 K；Ionic strength (raw mmCIF value) LOW；Pressure AMBIENT
NMR measurement conditions
pH 7；293 K；Ionic strength (raw mmCIF value) LOW；Pressure AMBIENT
NMR measurement conditions
pH 7；293 K；Ionic strength (raw mmCIF value) LOW；Pressure AMBIENT
NMR measurement conditions
pH 7；293 K；Ionic strength (raw mmCIF value) LOW；Pressure AMBIENT
NMR sample composition
1 MM HP67, 10 MM PHOSPHATE BUFFER, PH 7.0
NMR sample composition
1 MM HP67 U-15N , 10 MM PHOSPHATE BUFFER, PH 7.0
NMR sample composition
1 MM HP67 U-15N,10% 13C, 10 MM PHOSPHATE BUFFER, PH 7.0
NMR sample composition
1 MM HP67
|
Resolution not provided |
| 1VII THERMOSTABLE SUBDOMAIN FROM CHICKEN VILLIN HEADPIECE, NMR, MINIMIZED AVERAGE STRUCTURE Deposited 1997-01-15 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
792–826（35 aa）
Fragment：THERMOSTABLE SUBDOMAIN
|
Not recorded | No recorded non-water small molecule | SOLUTION NMR |
NMR measurement conditions
pH 3.7；303 K
|
Resolution not provided |
| 1WY3 Chicken villin subdomain HP-35, K65(NLE), N68H, pH7.0 Deposited 2005-02-04 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
792–826（35 aa）
Fragment：VHP
|
Mutation：K65(NLE), N68H Non-standard monomer：Yes (specific site not provided by mmCIF) | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 7；293 K；1ul 50mg/ml peptide plus 1ul (200mM KFormate, 20% PEG3350), pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K, pH 7.00
|
Resolution 0.95 Å R-free 0.171 |
| 1WY4 Chicken villin subdomain HP-35, K65(NLE), N68H, pH5.1 Deposited 2005-02-04 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
792–826（35 aa）
Fragment：VHP
|
Mutation：K65(NLE), N68H Non-standard monomer：Yes (specific site not provided by mmCIF) | IOD IODIDE ION × 2 NA SODIUM ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 5.1；293 K；1ul 50mg/ml peptide plus 1ul (200mM AmCitrate, 20% PEF3350), pH 5.1, VAPOR DIFFUSION, SITTING DROP, temperature 293K, pH 5.10
|
Resolution 1.55 Å R-free 0.213 |
| 1YRF Chicken villin subdomain HP-35, N68H, pH6.7 Deposited 2005-02-03 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
792–826（35 aa）
Fragment：VHP
|
Mutation：N68H | SO4 SULFATE ION × 1 ACT ACETATE ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 6.7；293 K；1ul 100mg/ml peptide plus 1ul (200mM NaOAc, 2.2M AmSO4), pH 6.7, VAPOR DIFFUSION, SITTING DROP, temperature 293K, pH 6.70
|
Resolution 1.07 Å R-free 0.161 |
| 1YRI Chicken villin subdomain HP-35, N68H, pH6.4 Deposited 2005-02-03 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
792–826（35 aa）
Fragment：VHP
|
Mutation：N68H | ACT ACETATE ION × 1 IOD IODIDE ION × 3 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 6.4；293 K；1ul 100mg/ml peptide plus 1ul (200mM AmOAc, 100mM NaCitrate, 30% PEG4000), pH 6.4, VAPOR DIFFUSION, SITTING DROP, temperature 293K, pH 6.40
|
Resolution 1.00 Å R-free 0.205 |
| 1YU5 Crystal Structure of the Headpiece Domain of Chicken Villin Deposited 2005-02-11 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain X
760–826（67 aa）
Fragment：Headpiece
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；298 K；1.5 M Na,K Phosphate buffer, 100 mM 2-(4-(2-Hydroxyethyl)-1-piperzainyl) ethanesulfonic acid, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 1.40 Å R-free 0.216 |
| 1YU7 Crystal Structure of the W64Y mutant of Villin Headpiece Deposited 2005-02-12 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain X
760–826（67 aa）
Fragment：Residue 10-76
|
Mutation：W64Y | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；298 K；0.2 M ammonium Sulfate, 0.1 M sodium cacodylate (pH 6.5) and 30 % polyethylene glycol (PEG) 8,000, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 1.50 Å R-free 0.210 |
| 1YU8 Crystal Structure of the R37A Mutant of Villin Headpiece Deposited 2005-02-12 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain X
760–826（67 aa）
Fragment：Residue 10-76
|
Mutation：R37A | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；298 K；0.2 M ammonium Sulfate, 0.1 M sodium cacodylate (pH 6.5) and 30 % polyethylene glycol (PEG) 8,000, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 1.45 Å R-free 0.211 |
| 2F4K Chicken villin subdomain HP-35, K65(NLE), N68H, K70(NLE), PH9 Deposited 2005-11-23 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
792–826（35 aa）
Fragment：residues 792-826
|
Mutation：N68H Non-standard monomer：Yes (specific site not provided by mmCIF) | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
Initial drop contained 1ul 90mg/ml protein in 10mM Tris (pH7.4) and 7.5% v/v trifluoroethanol (TFE) and 1ul reservoir solution. Precipitant in reservoir was (initial: 800mM AmSO4, 100mM Bicine (pH 9.0), 10% v/v TFE) and (final: 1.6M AmSO4, 100mM Bicine (pH 9.0), 7.5% v/v TFE). Reservoir solution was changed from initial to final after one week.
|
Resolution 1.05 Å R-free 0.166 |
| 2JM0 Solution structure of chicken villin headpiece subdomain containing a fluorinated side chain in the core Deposited 2006-09-06 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
792–826（35 aa）
Fragment：residues 792-826
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | No recorded non-water small molecule | SOLUTION NMR |
NMR measurement conditions
pH 5；298 K；Ionic strength (raw mmCIF value) 10；Pressure ambient
NMR sample composition
4 mM F5-Phe-cVHP | 93% H2O/7% D2O
NMR sample composition
2 mM selectively 15N-[Ala, Phe, Leu] F5-Phe-cVHP | 93% H2O/7% D2O
|
Resolution not provided |
| 2LLF Sixth Gelsolin-like domain of villin in 5 mM CaCl2 Deposited 2011-11-07 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
619–725（107 aa）
Fragment：Gelsolin-like 6 domain containing residues 619-725
|
Not recorded | No recorded non-water small molecule | SOLUTION NMR |
NMR measurement conditions
pH 7；298 K；Pressure ambient
NMR sample composition
50 M H2O, 5 M [U-2H] D2O, 10 mM [U-2H] DTT, 0.01 % sodium azide, 5 mM Calcium Chloride, 20 mM [U-2H] PIPES, 1 mM [U-99% 13C; U-99% 15N] D6, 90% H2O/10% D2O | 90% H2O/10% D2O
NMR sample composition
50 M H2O, 5 M [U-2H] D2O, 10 mM [U-2H] DTT, 0.01 % sodium azide, 5 mM Calcium Chloride, 20 mM [U-2H] PIPES, 1 mM D6, 90% H2O/10% D2O | 90% H2O/10% D2O
NMR sample composition
55 M [U-2H] D2O, 10 mM [U-2H] DTT, 0.01 % sodium azide, 5 mM Calcium Chloride, 20 mM [U-2H] PIPES, 1 mM D6, 100% D2O | 100% D2O
|
Resolution not provided |
| 2PPZ NMR solution Structure of the Villin Headpiece Mutant G34L Deposited 2007-05-01 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
792–826（35 aa）
Fragment：C-terminal residues of headpiece domain, sequence database residues 792-826
|
Mutation：G34L | No recorded non-water small molecule | SOLUTION NMR |
NMR measurement conditions
pH 5；298 K；Pressure ambient
NMR sample composition
4.3 mg peptide dissolved in 0.5 ml 90% H2O, 10% D2O (v/v) and 0.1 mM 2,2-dimethyl-2-silapentane sulfonic acid (DSS) for internal referencing | 90% H2O/10% D2O
|
Resolution not provided |
| 2RJV Crystal structure of the H41Y mutant of villin headpiece, P 21 21 21 space group Deposited 2007-10-15 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
760–826（67 aa）
Fragment：HEADPIECE
|
Mutation：H791Y | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；298 K；PEG8000, pH 6.50, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 1.45 Å R-free 0.206 |
| 2RJW The crystal structure of the H41Y mutant of villin headpiece, P61 SPACE GROUP. Deposited 2007-10-16 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
760–826（67 aa）
Fragment：VILLIN HEADPIECE
|
Mutation：H791Y | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；298 K；PEG 8000, pH 7.00, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 1.55 Å R-free 0.229 |
| 2RJW The crystal structure of the H41Y mutant of villin headpiece, P61 SPACE GROUP. Deposited 2007-10-16 | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain B
760–826（67 aa）
Fragment：VILLIN HEADPIECE
|
Mutation：H791Y | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；298 K；PEG 8000, pH 7.00, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 1.55 Å R-free 0.229 |
| 2RJX Crystal structure of the headpiece domain of chicken villin, P61 space group Deposited 2007-10-16 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
760–826（67 aa）
Fragment：VILLIN HEADPIECE
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；298 K；PEG 8000, pH 7.00, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 1.70 Å R-free 0.240 |
| 2RJX Crystal structure of the headpiece domain of chicken villin, P61 space group Deposited 2007-10-16 | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain B
760–826（67 aa）
Fragment：VILLIN HEADPIECE
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；298 K；PEG 8000, pH 7.00, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 1.70 Å R-free 0.240 |
| 2RJY Crystal structure of villin headpiece, P21 21 21 space group Deposited 2007-10-16 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
760–826（67 aa）
Fragment：VILLIN HEADPIECE
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；298 K；PEG 8000, pH 6.50, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 1.40 Å R-free 0.233 |
| 2VIK REFINED STRUCTURE OF THE ACTIN-SEVERING DOMAIN VILLIN 14T, DETERMINED BY SOLUTION NMR, MINIMIZED AVERAGE STRUCTURE Deposited 1997-01-16 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
2–127（126 aa）
Fragment：RESIDUES 1 - 126
|
Not recorded | No recorded non-water small molecule | SOLUTION NMR |
NMR measurement conditions
pH 4.15；298 K
|
Resolution not provided |
| 2VIL REFINED STRUCTURE OF THE ACTIN-SEVERING DOMAIN VILLIN 14T, DETERMINED BY SOLUTION NMR, 11 STRUCTURES Deposited 1997-01-16 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
2–127（126 aa）
Fragment：RESIDUES 1 - 126
|
Not recorded | No recorded non-water small molecule | SOLUTION NMR |
NMR measurement conditions
pH 4.15；298 K
|
Resolution not provided |
| 3MYA Crystal Structure of HP67 H41F - P61 Deposited 2010-05-10 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
760–826（67 aa）
Fragment：Villin Headpiece (unp residues 760-826)
|
Mutation：H41F | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；293 K；0.2 M Ammonium Sulfate, 0.1 M 2-morpholinoethanesulfonic Acid, 30% Polyethylene Glycol 8,000, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.50 Å R-free 0.277 |
| 3MYA Crystal Structure of HP67 H41F - P61 Deposited 2010-05-10 | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain B
760–826（67 aa）
Fragment：Villin Headpiece (unp residues 760-826)
|
Mutation：H41F | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；293 K；0.2 M Ammonium Sulfate, 0.1 M 2-morpholinoethanesulfonic Acid, 30% Polyethylene Glycol 8,000, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.50 Å R-free 0.277 |
| 3MYC Crystal Structure of HP67 H41F - P212121 Deposited 2010-05-10 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
760–826（67 aa）
Fragment：Villin Headpiece (UNP residues 760-826)
|
Mutation：H41F | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；293 K；0.2 M Ammonium Sulfate, 0.1 M 2-Morphinoethanesulfonic acid, 30% Polyethyleneglycol 8,000, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 1.70 Å R-free 0.216 |
| 3MYE Crystal Structure of HP67 L61GL Deposited 2010-05-10 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain X
760–826（67 aa）
Fragment：Villin Headpiece (unp residues 760-826)
|
Mutation：L61GL | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；293 K；0.2 M Sodium Acetate, 0.1 M Cacodylate, 30% Polyethylene Glycol 10,000, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 1.80 Å R-free 0.199 |
| 3NKJ Crystal Structure of HP67 L61G Deposited 2010-06-20 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
760–826（67 aa）
Fragment：Villin Headpiece (UNP Residues 760-826)
|
Mutation：L811G | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；277 K；0.2 M SODIUM ACETATE, 0.1 M CACODYLATE, 30% POLYETHYLENE GLYCOL 10,000, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 1.60 Å R-free 0.219 |
| 3TJW Crystal Structure of Quasiracemic Villin Headpiece Subdomain Containing (F5Phe10) Substitution Deposited 2011-08-25 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
792–825（34 aa）
Fragment：headpiece subdomain (UNP residues 792-825)
|
Mutation：N818H Non-standard monomer：Yes (specific site not provided by mmCIF) | SO4 SULFATE ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5.75；298 K；2.0 M ammonium sulfate, 6% isopropanol, 8% glycerol, pH 5.75, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K
|
Resolution 1.46 Å R-free 0.190 |
| 3TJW Crystal Structure of Quasiracemic Villin Headpiece Subdomain Containing (F5Phe10) Substitution Deposited 2011-08-25 | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain B
792–825（34 aa）
Fragment：headpiece subdomain (UNP residues 792-825)
|
Mutation：N818H Non-standard monomer：Yes (specific site not provided by mmCIF) | SO4 SULFATE ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5.75；298 K；2.0 M ammonium sulfate, 6% isopropanol, 8% glycerol, pH 5.75, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K
|
Resolution 1.46 Å R-free 0.190 |
| 3TRV Crystal structure of quasiracemic villin headpiece subdomain containing (F5Phe17) substitution Deposited 2011-09-11 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
792–826（35 aa）
Fragment：headpiece subdomain (UNP residues 792-826)
|
Mutation：N818H Non-standard monomer：Yes (specific site not provided by mmCIF) | SO4 SULFATE ION × 2 IPA ISOPROPYL ALCOHOL × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5.75；298 K；2.0 M ammonium sulfate, 6% isopropanol, 8% glycerol, pH 5.75, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 1.00 Å R-free 0.155 |
| 3TRV Crystal structure of quasiracemic villin headpiece subdomain containing (F5Phe17) substitution Deposited 2011-09-11 | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain B
792–826（35 aa）
Fragment：headpiece subdomain (UNP residues 792-826)
|
Mutation：N818H Non-standard monomer：Yes (specific site not provided by mmCIF) | SO4 SULFATE ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5.75；298 K；2.0 M ammonium sulfate, 6% isopropanol, 8% glycerol, pH 5.75, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 1.00 Å R-free 0.155 |
| 3TRW Crystal structure of racemic villin headpiece subdomain crystallized in space group P-1 Deposited 2011-09-11 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
792–826（35 aa）
Fragment：headpiece subdomain (UNP residues 792-826)
|
Mutation：N818H | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5.75；298 K；2.0 M ammonium sulfate, 6% isopropanol, 8% glycerol, pH 5.75, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 2.10 Å R-free 0.352 |
| 3TRW Crystal structure of racemic villin headpiece subdomain crystallized in space group P-1 Deposited 2011-09-11 | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain D
792–826（35 aa）
Fragment：headpiece subdomain (UNP residues 792-826)
|
Mutation：N818H | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5.75；298 K；2.0 M ammonium sulfate, 6% isopropanol, 8% glycerol, pH 5.75, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 2.10 Å R-free 0.352 |
| 3TRY Crystal structure of racemic villin headpiece subdomain in space group I-4c2 Deposited 2011-09-11 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
792–826（35 aa）
Fragment：headpiece subdomain (UNP residues 792-826)
|
Mutation：N818H Non-standard monomer：Yes (specific site not provided by mmCIF) | SO4 SULFATE ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 5.75；298 K；2.0 M ammonium sulfate, 6% isopropanol, pH 5.75, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 2.30 Å R-free 0.230 |
| 4CZ3 HP24wt derived from the villin headpiece subdomain Deposited 2014-04-16 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
803–826（24 aa）
Fragment：RESIDUES 803-826
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | No recorded non-water small molecule | SOLUTION NMR |
NMR measurement conditions
pH 7.5；288 K；Ionic strength (raw mmCIF value) 50；Pressure 1
NMR sample composition
10% D2O/90% WATER
|
Resolution not provided |
| 4CZ4 HP24stab derived from the villin headpiece subdomain Deposited 2014-04-16 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
803–826（24 aa）
Fragment：RESIDUES 803-826
|
Mutation：YES Non-standard monomer：Yes (specific site not provided by mmCIF) | No recorded non-water small molecule | SOLUTION NMR |
NMR measurement conditions
pH 7.5；288 K；Ionic strength (raw mmCIF value) 50；Pressure 1
NMR sample composition
10% D2O/90% WATER
|
Resolution not provided |
| 5I1N Villin headpiece subdomain with a Gln26 to beta-3-homoglutamine substitution Deposited 2016-02-05 | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric（8） Consistent with protein count |
Chain A
792–826（35 aa）
Chain B
792–826（35 aa）
Chain C
792–826（35 aa）
Chain D
792–826（35 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 4.6；298 K；0.1 M sodium acetate pH 4.6, 1.5 M ammonium sulfate, 30% (v/v) PEG2000MME
|
Resolution 1.30 Å R-free 0.231 |
| 5I1O Villin headpiece subdomain with a Gln26 to ACPC substitution Deposited 2016-02-05 | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric（8） Consistent with protein count |
Chain A
792–826（35 aa）
Chain B
792–826（35 aa）
Chain C
792–826（35 aa）
Chain D
792–826（35 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8.5；298 K；0.1 M Tris-HCl pH 8.5, 0.1 M sodium acetate, 30% (w/v) PEG 4000
|
Resolution 1.35 Å R-free 0.204 |
| 5I1P Villin headpiece subdomain with a Lys30 to beta-3-homolysine substitution Deposited 2016-02-05 | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric（8） Consistent with protein count |
Chain A
792–826（35 aa）
Chain B
792–826（35 aa）
Chain C
792–826（35 aa）
Chain D
792–826（35 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | EOH ETHANOL × 3 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8.5；298 K；0.1 M Tris-HCl pH 8.5, 20% (v/v) ethanol
|
Resolution 1.40 Å R-free 0.235 |
| 5I1S Villin headpiece subdomain with a Lys30 to APC substitution Deposited 2016-02-05 | Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain A
792–826（35 aa）
Chain B
792–826（35 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 4.6；298 K；0.1 M sodium acetate pH 4.6, 1.5 M ammonium sulfate, 30% (v/v) PEG2000MME
|
Resolution 1.12 Å R-free 0.185 |
| 9MF7 Backbone alpha-Methylation in the Villin Headpiece Miniprotein: Prototype HP35 Deposited 2024-12-09 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
792–826（35 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | No recorded non-water small molecule | SOLUTION NMR |
NMR measurement conditions
pH 5；293 K；Ionic strength (raw mmCIF value) 10；Pressure 1
NMR sample composition
0.52 mM Villin-1 headpiece: Met12Nle, 0.21 mM DSS, 90% H2O/10% D2O | 90% H2O/10% D2O
|
Resolution not provided |
| 9MF8 Backbone alpha-Methylation in the Villin Headpiece Miniprotein: HP35 with Aib at Position 15 Deposited 2024-12-09 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
792–826（35 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | No recorded non-water small molecule | SOLUTION NMR |
NMR measurement conditions
pH 5；288 K；Ionic strength (raw mmCIF value) 10；Pressure 1
NMR sample composition
0.47 mM Villin-1 headpiece: Met12Nle, Ser15Aib, 0.15 mM DSS, 90% H2O/10% D2O | 90% H2O/10% D2O
|
Resolution not provided |
| 9MF9 Backbone alpha-Methylation in the Villin Headpiece Miniprotein: HP35 with Calpha-methyl-Ser at Position 15 Deposited 2024-12-09 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
792–826（35 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | No recorded non-water small molecule | SOLUTION NMR |
NMR measurement conditions
pH 5；288 K；Ionic strength (raw mmCIF value) 10；Pressure 1
NMR sample composition
0.44 mM Villin-1 headpiece: Met12Nle, Ser15alphaMeSer, 0.15 mM DSS, 90% H2O/10% D2O | 90% H2O/10% D2O
|
Resolution not provided |
| 9MFA Backbone alpha-Methylation in the Villin Headpiece Miniprotein: HP35 with Aib at Position 30 Deposited 2024-12-09 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
792–826（35 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | No recorded non-water small molecule | SOLUTION NMR |
NMR measurement conditions
pH 5；288 K；Ionic strength (raw mmCIF value) 10；Pressure 1
NMR sample composition
0.59 mM Villin-1 headpiece: Met12Nle, Lys30Aib, 0.15 mM DSS, 90% H2O/10% D2O | 90% H2O/10% D2O
|
Resolution not provided |
| 9MFB Backbone alpha-Methylation in the Villin Headpiece Miniprotein: HP35 with Calpha-methyl-Lys at Position 30 Deposited 2024-12-09 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
792–826（35 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | No recorded non-water small molecule | SOLUTION NMR |
NMR measurement conditions
pH 5；288 K；Ionic strength (raw mmCIF value) 10；Pressure 1
NMR sample composition
0.53 mM Villin-1 headpiece: Met12Nle, Lys30alphaMeLys, 0.15 mM DSS, 90% H2O/10% D2O | 90% H2O/10% D2O
|
Resolution not provided |
| 9YLZ Backbone Modification in the Villin Headpiece Miniprotein: HP35 with beta3Leu at Position 28 Deposited 2025-10-09 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
792–826（35 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | No recorded non-water small molecule | SOLUTION NMR |
NMR measurement conditions
pH 5.1；283 K；Ionic strength (raw mmCIF value) 10；Pressure 1
NMR sample composition
0.96 mM Villin-1 headpiece: Met12Nle, Leu28beta3Leu, 0.2 mM DSS, 90% H2O/10% D2O | 90% H2O/10% D2O
|
Resolution not provided |
| 9YM0 Backbone Modification in the Villin Headpiece Miniprotein: HP35 with ACPC at Position 28 Deposited 2025-10-09 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
792–826（35 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | No recorded non-water small molecule | SOLUTION NMR |
NMR measurement conditions
pH 4.9；288 K；Ionic strength (raw mmCIF value) 10；Pressure 1
NMR sample composition
0.91 mM Villin-1 headpiece: Met12Nle, Leu28ACPC, 0.2 mM DSS, 90% H2O/10% D2O | 90% H2O/10% D2O
|
Resolution not provided |
| 9YM1 Backbone Modification in the Villin Headpiece Miniprotein: HP35 with beta3Lys at Position 29 Deposited 2025-10-09 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
792–826（35 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | No recorded non-water small molecule | SOLUTION NMR |
NMR measurement conditions
pH 5；278 K；Ionic strength (raw mmCIF value) 10；Pressure 1
NMR sample composition
0.89 mM Villin-1 headpiece: Met12Nle, Lys29beta3Lys, 0.2 mM DSS, 90% H2O/10% D2O | 90% H2O/10% D2O
|
Resolution not provided |
| 9YM2 Backbone Modification in the Villin Headpiece Miniprotein: HP35 with ACPC at Position 29 Deposited 2025-10-09 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
792–826（35 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | No recorded non-water small molecule | SOLUTION NMR |
NMR measurement conditions
pH 4.8；278 K；Ionic strength (raw mmCIF value) 10；Pressure 1
NMR sample composition
1.0 mM Villin-1 headpiece: Met12Nle, Lys29ACPC, 0.2 mM DSS, 90% H2O/10% D2O | 90% H2O/10% D2O
|
Resolution not provided |
| 9YM3 Backbone Modification in the Villin Headpiece Miniprotein: HP35 with ACPC at Position 30 Deposited 2025-10-09 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
792–826（35 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | No recorded non-water small molecule | SOLUTION NMR |
NMR measurement conditions
pH 5；288 K；Ionic strength (raw mmCIF value) 10；Pressure 1
NMR sample composition
1.0 mM Villin-1 headpiece: Met12Nle, Lys30ACPC, 0.2 mM DSS, 90% H2O/10% D2O | 90% H2O/10% D2O
|
Resolution not provided |
| 9YM4 Backbone Modification in the Villin Headpiece Miniprotein: HP35 with beta3Phe at Position 6 Deposited 2025-10-09 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
792–826（35 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | No recorded non-water small molecule | SOLUTION NMR |
NMR measurement conditions
pH 4.8；278 K；Ionic strength (raw mmCIF value) 10；Pressure 1
NMR sample composition
1.0 mM Villin-1 headpiece: Met12Nle, Phe6beta3Phe, 0.2 mM DSS, 90% H2O/10% D2O | 90% H2O/10% D2O
|
Resolution not provided |
| 9YM5 Backbone Modification in the Villin Headpiece Miniprotein: HP35 with Calpha-methyl-Phe at Position 6 Deposited 2025-10-09 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
792–826（35 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | No recorded non-water small molecule | SOLUTION NMR |
NMR measurement conditions
pH 4.7；293 K；Ionic strength (raw mmCIF value) 10；Pressure 1
NMR sample composition
1.0 mM Villin-1 headpiece: Met12Nle, Phe6alphaMePhe, 0.2 mM DSS, 90% H2O/10% D2O | 90% H2O/10% D2O
|
Resolution not provided |
| 9YM6 Backbone Modification in the Villin Headpiece Miniprotein: HP35 with Calpha-methyl-Phe at Position 17 Deposited 2025-10-09 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
792–826（35 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | No recorded non-water small molecule | SOLUTION NMR |
NMR measurement conditions
pH 5；288 K；Ionic strength (raw mmCIF value) 10；Pressure 1
NMR sample composition
1.0 mM Villin-1 headpiece: Met12Nle, Phe17alphaMePhe, 0.2 mM DSS, 90% H2O/10% D2O | 90% H2O/10% D2O
|
Resolution not provided |