当前蛋白身份：P17293
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差异标签只比较当前检索结果；所有PDB和assembly原始记录仍分别保留。
相关结构差异明细
一行代表一个 PDB 条目中的一个 biological assembly；同一蛋白的多个单体会分别列出。
| PDB 条目 | Assembly / 聚集状态 | 构建体 | 突变与修饰 | 配体、离子与非聚合物 | 实验方法 | 实验环境 | 结构质量 |
|---|---|---|---|---|---|---|---|
| 1FJG STRUCTURE OF THE THERMUS THERMOPHILUS 30S RIBOSOMAL SUBUNIT IN COMPLEX WITH THE ANTIBIOTICS STREPTOMYCIN, SPECTINOMYCIN, AND PAROMOMYCIN 提交 2000-08-08 | Assembly 1 蛋白–RNA 异源复合物；蛋白 × 20 PDB 声明：22-meric（22） 与全部聚合物一致 |
链 L
1–135（135 aa）
|
未记录 | PAR PAROMOMYCIN × 1 MG MAGNESIUM ION × 96 SCM SPECTINOMYCIN × 1 SRY STREPTOMYCIN × 1 ZN ZINC ION × 2 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 6.5；277 K；MPD, NH4Cl, KCl, CaCl2, magnesium acetate, sodium cacodylate,
streptomycin, paromomycin, spectinomycin, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
分辨率 3.00 Å R-free 0.255 |
| 1HNW STRUCTURE OF THE THERMUS THERMOPHILUS 30S RIBOSOMAL SUBUNIT IN COMPLEX WITH TETRACYCLINE 提交 2000-12-08 | Assembly 1 蛋白–RNA 异源复合物；蛋白 × 20 PDB 声明：22-meric（22） 与全部聚合物一致 |
链 L
1–135（135 aa）
|
未记录 | MG MAGNESIUM ION × 96 TAC TETRACYCLINE × 2 ZN ZINC ION × 2 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 6.5；277 K；VAPOR DIFFUSION, HANGING DROP at 277 K. MPD, NH4CL, KCL, CACL2, MAGNESIUM ACETATE, SODIUM CACODYLATE, pH 6.5. 80 mM tetracycline soaked into preformed crystals., pH 6.50
|
分辨率 3.40 Å R-free 0.264 |
| 1HNX STRUCTURE OF THE THERMUS THERMOPHILUS 30S RIBOSOMAL SUBUNIT IN COMPLEX WITH PACTAMYCIN 提交 2000-12-08 | Assembly 1 蛋白–RNA 异源复合物；蛋白 × 20 PDB 声明：22-meric（22） 与全部聚合物一致 |
链 L
1–135（135 aa）
|
未记录 | MG MAGNESIUM ION × 96 PCY Pactamycin × 1 ZN ZINC ION × 2 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 6.5；277 K；VAPOR DIFFUSION, HANGING DROP AT 277 K. MPD, NH4CL, KCL, CACL2, MAGNESIUM ACETATE,
SODIUM CACODYLATE, pH 6.50. 80 uM Pactamycin soaked into preformed crystals.
|
分辨率 3.40 Å R-free 0.280 |
| 1HNZ STRUCTURE OF THE THERMUS THERMOPHILUS 30S RIBOSOMAL SUBUNIT IN COMPLEX WITH HYGROMYCIN B 提交 2000-12-08 | Assembly 1 蛋白–RNA 异源复合物；蛋白 × 20 PDB 声明：22-meric（22） 与全部聚合物一致 |
链 L
1–135（135 aa）
|
未记录 | MG MAGNESIUM ION × 96 HYG HYGROMYCIN B × 1 ZN ZINC ION × 2 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 6.5；277 K；VAPOR DIFFUSION, HANGING DROP at 277 K. MPD, NH4CL, KCL, CACL2, MAGNESIUM ACETATE,
SODIUM CACODYLATE, pH 6.50. 80 uM HYGROMYCIN B soaked into preformed crystals.
|
分辨率 3.30 Å R-free 0.261 |
| 1HR0 CRYSTAL STRUCTURE OF INITIATION FACTOR IF1 BOUND TO THE 30S RIBOSOMAL SUBUNIT 提交 2000-12-20 | Assembly 1 蛋白–RNA 异源复合物；蛋白 × 21 PDB 声明：23-meric（23） 与全部聚合物一致 |
链 L
1–135（135 aa）
|
未记录 | MG MAGNESIUM ION × 65 ZN ZINC ION × 2 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 6.5；277 K；15% MPD, 25 mM magnesium Acetate, 200 mM KCl, 75 mM NH4Cl, 100 mM K-Cacodylate, pH 6.50. VAPOR DIFFUSION, HANGING DROP at 277 K
Initiation factor 1 soaked into pre-formed crystals.
|
分辨率 3.20 Å R-free 0.261 |
| 1I94 CRYSTAL STRUCTURES OF THE SMALL RIBOSOMAL SUBUNIT WITH TETRACYCLINE, EDEINE AND IF3 提交 2001-03-18 | Assembly 1 蛋白–RNA 异源复合物；蛋白 × 20 PDB 声明：21-meric（21） 与全部聚合物一致 |
链 L
5–135（131 aa）
|
未记录 | MG MAGNESIUM ION × 75 WO2 OCTADECATUNGSTENYL DIPHOSPHATE × 14 ZN ZINC ION × 2 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 7.8；291 K；MPD, magnesium chloride, spermidine, pH 7.8, VAPOR DIFFUSION, HANGING DROP, temperature 291.K
|
分辨率 3.20 Å R-free 0.245 |
| 1I95 CRYSTAL STRUCTURE OF THE 30S RIBOSOMAL SUBUNIT FROM THERMUS THERMOPHILUS IN COMPLEX WITH EDEINE 提交 2001-03-18 | Assembly 1 蛋白–RNA 异源复合物；蛋白 × 20 PDB 声明：21-meric（21） 与全部聚合物一致 |
链 L
5–135（131 aa）
|
未记录 | MG MAGNESIUM ION × 75 WO2 OCTADECATUNGSTENYL DIPHOSPHATE × 14 EDE EDEINE B × 1 ZN ZINC ION × 2 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 7.8；291 K；MPD, magnesium chloride, spermidine, pH 7.8, VAPOR DIFFUSION, HANGING DROP, temperature 291.K
|
分辨率 4.50 Å R-free 0.244 |
| 1I96 CRYSTAL STRUCTURE OF THE 30S RIBOSOMAL SUBUNIT FROM THERMUS THERMOPHILUS IN COMPLEX WITH THE TRANSLATION INITIATION FACTOR IF3 (C-TERMINAL DOMAIN) 提交 2001-03-18 | Assembly 1 蛋白–RNA 异源复合物；蛋白 × 21 PDB 声明：22-meric（22） 与全部聚合物一致 |
链 L
5–135（131 aa）
|
未记录 | MG MAGNESIUM ION × 75 WO2 OCTADECATUNGSTENYL DIPHOSPHATE × 12 ZN ZINC ION × 2 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 7.8；291 K；MPD, magnesium chloride, spermidine, pH 7.8, VAPOR DIFFUSION, HANGING DROP, temperature 291.K
|
分辨率 4.20 Å R-free 0.264 |
| 1I97 CRYSTAL STRUCTURE OF THE 30S RIBOSOMAL SUBUNIT FROM THERMUS THERMOPHILUS IN COMPLEX WITH TETRACYCLINE 提交 2001-03-18 | Assembly 1 蛋白–RNA 异源复合物；蛋白 × 20 PDB 声明：21-meric（21） 与全部聚合物一致 |
链 L
5–135（131 aa）
|
未记录 | MG MAGNESIUM ION × 75 WO2 OCTADECATUNGSTENYL DIPHOSPHATE × 14 TAC TETRACYCLINE × 6 ZN ZINC ION × 2 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 7.8；291 K；MPD, magnesium chloride, spermidine, pH 7.8, VAPOR DIFFUSION, HANGING DROP, temperature 291.K
|
分辨率 4.50 Å R-free 0.254 |
| 1IBK STRUCTURE OF THE THERMUS THERMOPHILUS 30S RIBOSOMAL SUBUNIT IN COMPLEX WITH THE ANTIBIOTIC PAROMOMYCIN 提交 2001-03-28 | Assembly 1 蛋白–RNA 异源复合物；蛋白 × 20 PDB 声明：22-meric（22） 与全部聚合物一致 |
链 L
1–135（135 aa）
|
未记录 | PAR PAROMOMYCIN × 1 MG MAGNESIUM ION × 113 ZN ZINC ION × 2 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 6.5；277 K；MPD, AMMONIUM CHLORIDE, POTASSIUM CHLORIDE, MAGNESIUM ACETATE, SODIUM CACODYLATE,
pH 6.5, VAPOR DIFFUSION, HANGING DROP at 277K, pH 6.50
|
分辨率 3.31 Å R-free 0.282 |
| 1IBL STRUCTURE OF THE THERMUS THERMOPHILUS 30S RIBOSOMAL SUBUNIT IN COMPLEX WITH A MESSENGER RNA FRAGMENT AND COGNATE TRANSFER RNA ANTICODON STEM-LOOP BOUND AT THE A SITE AND WITH THE ANTIBIOTIC PAROMOMYCIN 提交 2001-03-28 | Assembly 1 蛋白–RNA 异源复合物；蛋白 × 20 PDB 声明：24-meric（24） 与全部聚合物一致 |
链 L
1–135（135 aa）
|
未记录 | PAR PAROMOMYCIN × 1 MG MAGNESIUM ION × 125 ZN ZINC ION × 2 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 6.5；277 K；MPD, AMMONIUM CHLORIDE, POTASSIUM CHLORIDE, MAGNESIUM ACETATE, SODIUM CACODYLATE,
pH 6.5, VAPOR DIFFUSION, HANGING DROP at 277K, pH 6.50
|
分辨率 3.11 Å R-free 0.275 |
| 1IBM STRUCTURE OF THE THERMUS THERMOPHILUS 30S RIBOSOMAL SUBUNIT IN COMPLEX WITH A MESSENGER RNA FRAGMENT AND COGNATE TRANSFER RNA ANTICODON STEM-LOOP BOUND AT THE A SITE 提交 2001-03-28 | Assembly 1 蛋白–RNA 异源复合物；蛋白 × 20 PDB 声明：24-meric（24） 与全部聚合物一致 |
链 L
1–135（135 aa）
|
未记录 | MG MAGNESIUM ION × 120 ZN ZINC ION × 2 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 6.5；277 K；MPD, AMMONIUM CHLORIDE, POTASSIUM CHLORIDE, MAGNESIUM ACETATE, SODIUM CACODYLATE
pH 6.5, VAPOR DIFFUSION, HANGING DROP at 277K, pH 6.50
|
分辨率 3.31 Å R-free 0.286 |
| 1J5E Structure of the Thermus thermophilus 30S Ribosomal Subunit 提交 2002-04-08 | Assembly 1 蛋白–RNA 异源复合物；蛋白 × 20 PDB 声明：21-meric（21） 与全部聚合物一致 |
链 L
1–135（135 aa）
|
未记录 | UNX UNKNOWN LIGAND × 188 ZN ZINC ION × 2 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 6.5；277 K；MPD, NH4Cl, KCl, CaCl2, magnesium acetate, sodium cacodylate, pH 6.5,
VAPOR DIFFUSION, HANGING DROP at 277K
|
分辨率 3.05 Å R-free 0.252 |
| 1N32 Structure of the Thermus thermophilus 30S ribosomal subunit bound to codon and near-cognate transfer RNA anticodon stem-loop mismatched at the first codon position at the a site with paromomycin 提交 2002-10-25 | Assembly 1 蛋白–RNA 异源复合物；蛋白 × 20 PDB 声明：23-meric（23） 与全部聚合物一致 |
链 L
1–135（135 aa）
|
未记录 | PAR PAROMOMYCIN × 1 MG MAGNESIUM ION × 158 ZN ZINC ION × 2 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 6.5；277 K；MPD, NH4Cl, KCl, CaCl2, magnesium acetate, potassium-MES, sodium cacodylate,
PH 6.5, VAPOR DIFFUSION, HANGING DROP AT 277K
|
分辨率 3.00 Å R-free 0.270 |
| 1N33 Structure of the Thermus thermophilus 30S ribosomal subunit bound to codon and near-cognate transfer rna anticodon stem-loop mismatched at the second codon position at the a site with paromomycin 提交 2002-10-25 | Assembly 1 蛋白–RNA 异源复合物；蛋白 × 20 PDB 声明：23-meric（23） 与全部聚合物一致 |
链 L
1–135（135 aa）
|
未记录 | PAR PAROMOMYCIN × 1 MG MAGNESIUM ION × 106 ZN ZINC ION × 2 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 6.5；277 K；MPD, NH4Cl, KCl, CaCl2, magnesium acetate, potassium-MES, sodium cacodylate,
PH 6.5, VAPOR DIFFUSION, HANGING DROP AT 277K
|
分辨率 3.35 Å R-free 0.284 |
| 2F4V 30S ribosome + designer antibiotic 提交 2005-11-24 | Assembly 1 蛋白–RNA 异源复合物；蛋白 × 19 PDB 声明：21-meric（21） 与全部聚合物一致 |
链 L
1–131（131 aa）
|
未记录 | MG MAGNESIUM ION × 101 K POTASSIUM ION × 12 D2C (2S,4S,4AR,5AS,6S,11R,11AS,12R,12AR)-7-CHLORO-4-(DIMETHYLAMINO)-6,10,11,12-TETRAHYDROXY-1,3-DIOXO-1,2,3,4,4A,5,5A,6,11,11A,12,12A-DODECAHYDROTETRACENE-2-CARBOXAMIDE × 1 AB9 (2R)-4-AMINO-N-{(1R,2S,3R,4R,5S)-5-AMINO-2-{2-[(2-AMINOETHYL)AMINO]ETHOXY}-4-[(2,6-DIAMINO-2,6-DIDEOXY-ALPHA-D-GLUCOPYRANOSYL)OXY]-3-HYDROXYCYCLOHEXYL}-2-HYDROXYBUTANAMIDE × 1 ZN ZINC ION × 2 | X-RAY DIFFRACTION | mmCIF 未提供已读取条件 | 分辨率 3.80 Å R-free 0.315 |
| 2HHH Crystal structure of kasugamycin bound to the 30S ribosomal subunit 提交 2006-06-28 | Assembly 1 蛋白–RNA 异源复合物；蛋白 × 20 PDB 声明：21-meric（21） 与全部聚合物一致 |
链 L
0–131（132 aa）
|
未记录 | KSG (1S,2R,3S,4R,5S,6S)-2,3,4,5,6-PENTAHYDROXYCYCLOHEXYL 2-AMINO-4-{[CARBOXY(IMINO)METHYL]AMINO}-2,3,4,6-TETRADEOXY-ALPHA-D-ARABINO-HEXOPYRANOSIDE × 2 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 6.5；300 K；MPD, MAGNESIUM CHLORIDE, POTASSIUM CHLORIDE, AMMONIUM CHLORIDE, MES-KOH, pH 6.50, VAPOR DIFFUSION, HANGING DROP, temperature 300K
|
分辨率 3.35 Å R-free 0.289 |
| 2OM7 Structural Basis for Interaction of the Ribosome with the Switch Regions of GTP-bound Elongation Factors 提交 2007-01-21 | Assembly 1 蛋白–RNA 异源复合物；蛋白 × 4 PDB 声明：tetradecameric（14） 与全部聚合物一致 |
链 E
1–132（132 aa）
|
未记录 | 未记录非水小分子 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
0.3 mM GMPPNP, 10 mM Hepes-KOH (pH 7.8), 10 mM Mg acetate, 60 mM NH4Cl, and 6 mM B-mercaptoethanol；pH 7.8；0.3 mM GMPPNP, 10 mM Hepes-KOH (pH 7.8), 10 mM Mg acetate, 60 mM NH4Cl, and 6 mM B-mercaptoethanol
cryo-EM玻璃化条件
冷冻剂 ETHANE
|
分辨率 7.30 Å |
| 2R1G Coordinates of the thermus thermophilus 30S components neighboring RbfA as obtained by fitting into the CRYO-EM map of A 30S-RBFA complex 提交 2007-08-22 | Assembly 1 蛋白–RNA 异源复合物；蛋白 × 3 PDB 声明：decameric（10） 与全部聚合物一致 |
链 H
2–125（124 aa）
片段：residues 5-128
|
未记录 | 未记录非水小分子 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
20mM, Hepes-KOH (pH 7.8), 10mM Mg(OAc)2, 200mM NH4Cl, 65mM KCl；pH 7.8；20mM, Hepes-KOH (pH 7.8), 10mM Mg(OAc)2, 200mM NH4Cl, 65mM KCl
cryo-EM玻璃化条件
冷冻剂 ETHANE；RAPID-FREEZING IN LIQUID ETHANE
|
分辨率 12.50 Å |
| 3OTO Crystal Structure of the 30S ribosomal subunit from a KsgA mutant of Thermus thermophilus (HB8) 提交 2010-09-13 | Assembly 1 蛋白–RNA 异源复合物；蛋白 × 20 PDB 声明：21-meric（21） 与全部聚合物一致 |
链 L
1–135（135 aa）
|
未记录 | MG MAGNESIUM ION × 94 K POTASSIUM ION × 42 ZN ZINC ION × 2 | X-RAY DIFFRACTION |
X-ray结晶条件
hanging drop；pH 6.5；277 K；pH 6.5, hanging drop, temperature 277K
|
分辨率 3.69 Å R-free 0.231 |
| 4AQY Structure of ribosome-apramycin complexes 提交 2012-04-20 | Assembly 1 蛋白–RNA 异源复合物；蛋白 × 20 PDB 声明：23-meric（23） 与全部聚合物一致 |
链 L
1–135（135 aa）
|
未记录 | MG MAGNESIUM ION × 203 K POTASSIUM ION × 15 AM2 APRAMYCIN × 5 ZN ZINC ION × 2 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；277 K；MPD, NH4CL, KCL, CACL2, MAGNESIUM ACETATE, SODIUM CACODYLATE, PH 6.5, VAPOR DIFFUSION, HANGING DROP AT 277K
|
分辨率 3.50 Å R-free 0.235 |
| 4V4Y Crystal structure of the 70S Thermus thermophilus ribosome with translocated and rotated Shine-Dalgarno Duplex. 提交 2006-06-27 | Assembly 1 蛋白–RNA 异源复合物；蛋白 × 50 PDB 声明：57-meric（57） 与全部聚合物一致 |
链 AO
0–131（132 aa）
|
未记录 | 未记录非水小分子 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 7.5；293 K；MPD, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
分辨率 5.50 Å R-free 0.326 |
| 4V4Z 70S Thermus thermophilous ribosome functional complex with mRNA and E- and P-site tRNAs at 4.5A. 提交 2006-06-27 | Assembly 1 蛋白–RNA 异源复合物；蛋白 × 50 PDB 声明：56-meric（56） 与全部聚合物一致 |
链 AO
0–131（132 aa）
|
未记录 | 未记录非水小分子 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 7.5；293 K；MPD, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
分辨率 4.51 Å R-free 0.345 |
| 4V5L The structure of EF-Tu and aminoacyl-tRNA bound to the 70S ribosome with a GTP analog 提交 2010-09-02 | Assembly 1 蛋白–RNA 异源复合物；蛋白 × 52 PDB 声明：59-meric（59） 与全部聚合物一致 |
链 AL
2–132（131 aa）
|
未记录 | PAR PAROMOMYCIN × 1 ZN ZINC ION × 4 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
pH 6.3；100 MM MES PH 6.3, 60-100 MM KCL, 50 MM SUCROSE, 1% GLYCEROL, AND 5.3% (W/V) PEG20K
|
分辨率 3.10 Å R-free 0.268 |
| 4V7J Structure of RelE nuclease bound to the 70S ribosome (precleavage state) 提交 2009-11-02 | Assembly 1 信息不足 异源复合物；蛋白 × 52 PDB 声明：58-meric（58） 与全部聚合物一致 |
链 Al
1–132（132 aa）
|
未记录 | ZN ZINC ION × 4 MG MAGNESIUM ION × 532 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 7.2；293 K；0.1M Tris-HAc, 0.2M KSCN, 3-4.5% w/v PEG 20k, 3-4.5% PEG 550 monomethylether, pH 7.2, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
分辨率 3.30 Å R-free 0.247 |
| 4V7J Structure of RelE nuclease bound to the 70S ribosome (precleavage state) 提交 2009-11-02 | Assembly 2 信息不足 异源复合物；蛋白 × 52 PDB 声明：58-meric（58） 与全部聚合物一致 |
链 未唯一映射
参考区间未声明
|
未记录 | ZN ZINC ION × 4 MG MAGNESIUM ION × 532 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 7.2；293 K；0.1M Tris-HAc, 0.2M KSCN, 3-4.5% w/v PEG 20k, 3-4.5% PEG 550 monomethylether, pH 7.2, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
分辨率 3.30 Å R-free 0.247 |
| 4V8X Structure of Thermus thermophilus ribosome 提交 2013-07-19 | Assembly 1 信息不足 异源复合物；蛋白 × 53 PDB 声明：59-meric（59） 与全部聚合物一致 |
链 AL
1–132（132 aa）
|
未记录 | MG MAGNESIUM ION × 352 ZN ZINC ION × 2 | X-RAY DIFFRACTION |
X-ray结晶条件
pH 7.1；0.1 M TRIS-HAC PH 7.2, 0.2 M KSCN, 4.1%-4.3% (W/V) PEG 20K AND 4.1%-4.3% (W/V) PEG 550MME
|
分辨率 3.35 Å R-free 0.261 |
| 4V8X Structure of Thermus thermophilus ribosome 提交 2013-07-19 | Assembly 2 信息不足 异源复合物；蛋白 × 53 PDB 声明：59-meric（59） 与全部聚合物一致 |
链 未唯一映射
参考区间未声明
|
未记录 | MG MAGNESIUM ION × 355 ZN ZINC ION × 2 | X-RAY DIFFRACTION |
X-ray结晶条件
pH 7.1；0.1 M TRIS-HAC PH 7.2, 0.2 M KSCN, 4.1%-4.3% (W/V) PEG 20K AND 4.1%-4.3% (W/V) PEG 550MME
|
分辨率 3.35 Å R-free 0.261 |
| 4WR6 Complex of 70S ribosome with tRNA-Tyr and mRNA with A-A mismatch in the first position in the A-site. 提交 2014-10-23 | Assembly 1 蛋白–RNA 异源复合物；蛋白 × 47 PDB 声明：54-MERIC（54） 与全部聚合物一致 |
链 3I
1–132（132 aa）
|
未记录 | MG MAGNESIUM ION × 652 ZN ZINC ION × 3 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP；pH 7；298 K；Tris-acetate, KSCN, PEG 550MME, PEG 20K
|
分辨率 3.05 Å R-free 0.249 |
| 4WR6 Complex of 70S ribosome with tRNA-Tyr and mRNA with A-A mismatch in the first position in the A-site. 提交 2014-10-23 | Assembly 2 蛋白–RNA 异源复合物；蛋白 × 47 PDB 声明：54-MERIC（54） 与全部聚合物一致 |
链 3A
1–132（132 aa）
|
未记录 | MG MAGNESIUM ION × 508 ZN ZINC ION × 3 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP；pH 7；298 K；Tris-acetate, KSCN, PEG 550MME, PEG 20K
|
分辨率 3.05 Å R-free 0.249 |
| 4WRA Complex of 70S ribosome with tRNA-Tyr and mRNA with A-A mismatch in the first position in the A-site and with antibiotic paromomycin. 提交 2014-10-23 | Assembly 1 蛋白–RNA 异源复合物；蛋白 × 47 PDB 声明：54-MERIC（54） 与全部聚合物一致 |
链 3I
1–132（132 aa）
|
未记录 | MG MAGNESIUM ION × 661 PAR PAROMOMYCIN × 1 ZN ZINC ION × 3 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP；pH 7；298 K；Tris-acetate, KSCN, PEG 550MME, PEG 20K
|
分辨率 3.05 Å R-free 0.249 |
| 4WRA Complex of 70S ribosome with tRNA-Tyr and mRNA with A-A mismatch in the first position in the A-site and with antibiotic paromomycin. 提交 2014-10-23 | Assembly 2 蛋白–RNA 异源复合物；蛋白 × 47 PDB 声明：54-MERIC（54） 与全部聚合物一致 |
链 3A
1–132（132 aa）
|
未记录 | MG MAGNESIUM ION × 497 PAR PAROMOMYCIN × 1 ZN ZINC ION × 3 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP；pH 7；298 K；Tris-acetate, KSCN, PEG 550MME, PEG 20K
|
分辨率 3.05 Å R-free 0.249 |
| 4WU1 Complex of 70S ribosome with tRNA-Tyr and mRNA with G-U mismatch in the second position in the P-site 提交 2014-10-30 | Assembly 1 蛋白–RNA 异源复合物；蛋白 × 48 PDB 声明：54-meric（54） 与全部聚合物一致 |
链 3I
1–132（132 aa）
|
未记录 | MG MAGNESIUM ION × 708 ZN ZINC ION × 3 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP；298 K；Tris-acetate, KSCN, PEG 550MME, PEG 20K
|
分辨率 3.20 Å R-free 0.238 |
| 4WU1 Complex of 70S ribosome with tRNA-Tyr and mRNA with G-U mismatch in the second position in the P-site 提交 2014-10-30 | Assembly 2 蛋白–RNA 异源复合物；蛋白 × 48 PDB 声明：54-meric（54） 与全部聚合物一致 |
链 3A
1–132（132 aa）
|
未记录 | MG MAGNESIUM ION × 673 ZN ZINC ION × 3 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP；298 K；Tris-acetate, KSCN, PEG 550MME, PEG 20K
|
分辨率 3.20 Å R-free 0.238 |
| 4WZD Complex of 70S ribosome with cognate tRNA-Tyr in the P-site 提交 2014-11-19 | Assembly 1 蛋白–RNA 异源复合物；蛋白 × 49 PDB 声明：55-meric（55） 与全部聚合物一致 |
链 3I
1–132（132 aa）
|
未记录 | MG MAGNESIUM ION × 699 ZN ZINC ION × 3 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP；298 K；Tris-acetate, KSCN, PEG 550MME, PEG 20K
|
分辨率 3.10 Å R-free 0.193 |
| 4WZD Complex of 70S ribosome with cognate tRNA-Tyr in the P-site 提交 2014-11-19 | Assembly 2 蛋白–RNA 异源复合物；蛋白 × 49 PDB 声明：55-meric（55） 与全部聚合物一致 |
链 3A
1–132（132 aa）
|
未记录 | MG MAGNESIUM ION × 646 ZN ZINC ION × 3 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP；298 K；Tris-acetate, KSCN, PEG 550MME, PEG 20K
|
分辨率 3.10 Å R-free 0.193 |
| 6C5L Conformation of methylated GGQ in the Peptidyl Transferase Center during translation termination (T. thermophilus) 提交 2018-01-16 | Assembly 1 蛋白–RNA 异源复合物；蛋白 × 53 PDB 声明：59-meric（59） 与全部聚合物一致 |
链 AL
1–132（132 aa）
|
未记录 | MG MAGNESIUM ION × 218 ZN ZINC ION × 3 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP；pH 7.5；292 K；5 mM HEPES, pH 7.5, 10 mM magnesium acetate, 50 mM potassium chloride, 10 mM ammonium chloride, 6 mM BME
|
分辨率 3.20 Å R-free 0.247 |
| 6C5L Conformation of methylated GGQ in the Peptidyl Transferase Center during translation termination (T. thermophilus) 提交 2018-01-16 | Assembly 2 蛋白–RNA 异源复合物；蛋白 × 53 PDB 声明：59-meric（59） 与全部聚合物一致 |
链 CL
1–132（132 aa）
|
未记录 | MG MAGNESIUM ION × 256 ZN ZINC ION × 3 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP；pH 7.5；292 K；5 mM HEPES, pH 7.5, 10 mM magnesium acetate, 50 mM potassium chloride, 10 mM ammonium chloride, 6 mM BME
|
分辨率 3.20 Å R-free 0.247 |
| 6CFJ Crystal structure of the Thermus thermophilus 70S ribosome in complex with histidyl-CAM and bound to mRNA and A-, P-, and E-site tRNAs at 2.8A resolution 提交 2018-02-15 | Assembly 1 蛋白–RNA 异源复合物；蛋白 × 49 PDB 声明：56-meric（56） 与全部聚合物一致 |
链 1l
1–132（132 aa）
|
非标准单体：是（mmCIF未提供具体位点） | MG MAGNESIUM ION × 1492 K POTASSIUM ION × 2 EZG N-[(1R,2R)-1,3-dihydroxy-1-(4-nitrophenyl)propan-2-yl]-L-histidinamide × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
|
分辨率 2.80 Å R-free 0.269 |
| 6CFJ Crystal structure of the Thermus thermophilus 70S ribosome in complex with histidyl-CAM and bound to mRNA and A-, P-, and E-site tRNAs at 2.8A resolution 提交 2018-02-15 | Assembly 2 蛋白–RNA 异源复合物；蛋白 × 49 PDB 声明：56-meric（56） 与全部聚合物一致 |
链 2l
1–132（132 aa）
|
非标准单体：是（mmCIF未提供具体位点） | MG MAGNESIUM ION × 1113 K POTASSIUM ION × 2 EZG N-[(1R,2R)-1,3-dihydroxy-1-(4-nitrophenyl)propan-2-yl]-L-histidinamide × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
|
分辨率 2.80 Å R-free 0.269 |
| 6CFK Crystal structure of the Thermus thermophilus 70S ribosome in complex with D-histidyl-CAM and bound to protein Y (YfiA) at 2.7A resolution 提交 2018-02-15 | Assembly 1 蛋白–RNA 异源复合物；蛋白 × 50 PDB 声明：53-meric（53） 与全部聚合物一致 |
链 1l
1–132（132 aa）
|
非标准单体：是（mmCIF未提供具体位点） | MG MAGNESIUM ION × 1422 K POTASSIUM ION × 1 EZP N-[(1R,2R)-1,3-dihydroxy-1-(4-nitrophenyl)propan-2-yl]-D-histidinamide × 1 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 4 ARG ARGININE × 2 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
|
分辨率 2.70 Å R-free 0.253 |
| 6CFK Crystal structure of the Thermus thermophilus 70S ribosome in complex with D-histidyl-CAM and bound to protein Y (YfiA) at 2.7A resolution 提交 2018-02-15 | Assembly 2 蛋白–RNA 异源复合物；蛋白 × 50 PDB 声明：53-meric（53） 与全部聚合物一致 |
链 2l
1–132（132 aa）
|
非标准单体：是（mmCIF未提供具体位点） | MG MAGNESIUM ION × 940 K POTASSIUM ION × 1 EZP N-[(1R,2R)-1,3-dihydroxy-1-(4-nitrophenyl)propan-2-yl]-D-histidinamide × 1 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 3 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
|
分辨率 2.70 Å R-free 0.253 |
| 6CFL Crystal structure of the Thermus thermophilus 70S ribosome in complex with lysyl-CAM and bound to protein Y (YfiA) at 2.6A resolution 提交 2018-02-15 | Assembly 1 蛋白–RNA 异源复合物；蛋白 × 50 PDB 声明：53-meric（53） 与全部聚合物一致 |
链 1l
1–132（132 aa）
|
非标准单体：是（mmCIF未提供具体位点） | MG MAGNESIUM ION × 1474 K POTASSIUM ION × 1 EZM N-[(1R,2R)-1,3-dihydroxy-1-(4-nitrophenyl)propan-2-yl]-L-lysinamide × 1 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 4 ARG ARGININE × 2 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
|
分辨率 2.60 Å R-free 0.252 |
| 6CFL Crystal structure of the Thermus thermophilus 70S ribosome in complex with lysyl-CAM and bound to protein Y (YfiA) at 2.6A resolution 提交 2018-02-15 | Assembly 2 蛋白–RNA 异源复合物；蛋白 × 50 PDB 声明：53-meric（53） 与全部聚合物一致 |
链 2l
1–132（132 aa）
|
非标准单体：是（mmCIF未提供具体位点） | MG MAGNESIUM ION × 955 K POTASSIUM ION × 1 EZM N-[(1R,2R)-1,3-dihydroxy-1-(4-nitrophenyl)propan-2-yl]-L-lysinamide × 1 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 3 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
|
分辨率 2.60 Å R-free 0.252 |
| 6N9E Crystal structure of the Thermus thermophilus 70S ribosome in complex with a short substrate mimic CC-Pmn and bound to mRNA and P-site tRNA at 3.7A resolution 提交 2018-12-03 | Assembly 1 蛋白–RNA 异源复合物；蛋白 × 49 PDB 声明：55-meric（55） 与全部聚合物一致 |
链 1l
1–132（132 aa）
|
非标准单体：是（mmCIF未提供具体位点） | MG MAGNESIUM ION × 632 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
|
分辨率 3.70 Å R-free 0.278 |
| 6N9E Crystal structure of the Thermus thermophilus 70S ribosome in complex with a short substrate mimic CC-Pmn and bound to mRNA and P-site tRNA at 3.7A resolution 提交 2018-12-03 | Assembly 2 蛋白–RNA 异源复合物；蛋白 × 49 PDB 声明：55-meric（55） 与全部聚合物一致 |
链 2l
1–132（132 aa）
|
非标准单体：是（mmCIF未提供具体位点） | MG MAGNESIUM ION × 524 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
|
分辨率 3.70 Å R-free 0.278 |
| 6N9F Crystal structure of the Thermus thermophilus 70S ribosome in complex with a short substrate mimic ACCA-DPhe and bound to mRNA and P-site tRNA at 3.7A resolution 提交 2018-12-03 | Assembly 1 蛋白–RNA 异源复合物；蛋白 × 49 PDB 声明：55-meric（55） 与全部聚合物一致 |
链 1l
1–132（132 aa）
|
非标准单体：是（mmCIF未提供具体位点） | MG MAGNESIUM ION × 710 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
|
分辨率 3.70 Å R-free 0.279 |
| 6N9F Crystal structure of the Thermus thermophilus 70S ribosome in complex with a short substrate mimic ACCA-DPhe and bound to mRNA and P-site tRNA at 3.7A resolution 提交 2018-12-03 | Assembly 2 蛋白–RNA 异源复合物；蛋白 × 49 PDB 声明：55-meric（55） 与全部聚合物一致 |
链 2l
1–132（132 aa）
|
非标准单体：是（mmCIF未提供具体位点） | MG MAGNESIUM ION × 629 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
|
分辨率 3.70 Å R-free 0.279 |