1hnx

STRUCTURE OF THE THERMUS THERMOPHILUS 30S RIBOSOMAL SUBUNIT IN COMPLEX WITH PACTAMYCIN

Method: X-RAY DIFFRACTION Dmax: 234.1 Å Quality: GOOD

1. Protein Identity and Related Structures Protein Identity & Related Structures

30S RIBOSOMAL PROTEIN S4

OrganismNot specified

UniProt P80373

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein–RNA Heteromer Protein × 20 RNA 2 PDB declaration: 22-meric(22) Consistent with all polymer counts Chain D; UniProt 1–209 Not recorded 16S RIBOSOMAL RNA × 1 FRAGMENT OF MESSENGER RNA × 1 30S RIBOSOMAL PROTEIN S2 × 1 30S RIBOSOMAL PROTEIN S3 × 1 30S RIBOSOMAL PROTEIN S5 × 1 (P27152) 30S RIBOSOMAL PROTEIN S6 × 1 (P23370) 30S RIBOSOMAL PROTEIN S7 × 1 (P17291) 30S RIBOSOMAL PROTEIN S8 × 1 (P24319) 30S RIBOSOMAL PROTEIN S9 × 1 30S RIBOSOMAL PROTEIN S10 × 1 (P80375) 30S RIBOSOMAL PROTEIN S11 × 1 30S RIBOSOMAL PROTEIN S12 × 1 (P17293) 30S RIBOSOMAL PROTEIN S13 × 1 30S RIBOSOMAL PROTEIN S14 × 1 (P24320) 30S RIBOSOMAL PROTEIN S15 × 1 (P80378) 30S RIBOSOMAL PROTEIN S16 × 1 30S RIBOSOMAL PROTEIN S17 × 1 30S RIBOSOMAL PROTEIN S18 × 1 30S RIBOSOMAL PROTEIN S19 × 1 (P80381) 30S RIBOSOMAL PROTEIN S20 × 1 30S RIBOSOMAL PROTEIN THX × 1 (P32193) MG MAGNESIUM ION × 96 PCY Pactamycin × 1 ZN ZINC ION × 2 X-RAY DIFFRACTION X-ray crystallization conditions:VAPOR DIFFUSION, HANGING DROP;pH 6.5;277 K;VAPOR DIFFUSION, HANGING DROP AT 277 K. MPD, NH4CL, KCL, CACL2, MAGNESIUM ACETATE, SODIUM CACODYLATE, pH 6.50. 80 uM Pactamycin soaked into preformed crystals. Resolution 3.40 Å R-free 0.280

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

391 other PDB entries and 611 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name RS4_THETH
Isoform
PDB entities 5
Chains and sequence ranges Author chain D; PDBConstruct 1–209; UniProt 1–209

30S RIBOSOMAL PROTEIN S5

OrganismNot specified

UniProt P27152

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein–RNA Heteromer Protein × 20 RNA 2 PDB declaration: 22-meric(22) Consistent with all polymer counts Chain E; UniProt 1–162 Not recorded 16S RIBOSOMAL RNA × 1 FRAGMENT OF MESSENGER RNA × 1 30S RIBOSOMAL PROTEIN S2 × 1 30S RIBOSOMAL PROTEIN S3 × 1 30S RIBOSOMAL PROTEIN S4 × 1 (P80373) 30S RIBOSOMAL PROTEIN S6 × 1 (P23370) 30S RIBOSOMAL PROTEIN S7 × 1 (P17291) 30S RIBOSOMAL PROTEIN S8 × 1 (P24319) 30S RIBOSOMAL PROTEIN S9 × 1 30S RIBOSOMAL PROTEIN S10 × 1 (P80375) 30S RIBOSOMAL PROTEIN S11 × 1 30S RIBOSOMAL PROTEIN S12 × 1 (P17293) 30S RIBOSOMAL PROTEIN S13 × 1 30S RIBOSOMAL PROTEIN S14 × 1 (P24320) 30S RIBOSOMAL PROTEIN S15 × 1 (P80378) 30S RIBOSOMAL PROTEIN S16 × 1 30S RIBOSOMAL PROTEIN S17 × 1 30S RIBOSOMAL PROTEIN S18 × 1 30S RIBOSOMAL PROTEIN S19 × 1 (P80381) 30S RIBOSOMAL PROTEIN S20 × 1 30S RIBOSOMAL PROTEIN THX × 1 (P32193) MG MAGNESIUM ION × 96 PCY Pactamycin × 1 ZN ZINC ION × 2 X-RAY DIFFRACTION X-ray crystallization conditions:VAPOR DIFFUSION, HANGING DROP;pH 6.5;277 K;VAPOR DIFFUSION, HANGING DROP AT 277 K. MPD, NH4CL, KCL, CACL2, MAGNESIUM ACETATE, SODIUM CACODYLATE, pH 6.50. 80 uM Pactamycin soaked into preformed crystals. Resolution 3.40 Å R-free 0.280

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

39 other PDB entries and 49 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name RS5_THETH
Isoform
PDB entities 6
Chains and sequence ranges Author chain E; PDBConstruct 1–162; UniProt 1–162

30S RIBOSOMAL PROTEIN S6

OrganismNot specified

UniProt P23370

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein–RNA Heteromer Protein × 20 RNA 2 PDB declaration: 22-meric(22) Consistent with all polymer counts Chain F; UniProt 1–101 Not recorded 16S RIBOSOMAL RNA × 1 FRAGMENT OF MESSENGER RNA × 1 30S RIBOSOMAL PROTEIN S2 × 1 30S RIBOSOMAL PROTEIN S3 × 1 30S RIBOSOMAL PROTEIN S4 × 1 (P80373) 30S RIBOSOMAL PROTEIN S5 × 1 (P27152) 30S RIBOSOMAL PROTEIN S7 × 1 (P17291) 30S RIBOSOMAL PROTEIN S8 × 1 (P24319) 30S RIBOSOMAL PROTEIN S9 × 1 30S RIBOSOMAL PROTEIN S10 × 1 (P80375) 30S RIBOSOMAL PROTEIN S11 × 1 30S RIBOSOMAL PROTEIN S12 × 1 (P17293) 30S RIBOSOMAL PROTEIN S13 × 1 30S RIBOSOMAL PROTEIN S14 × 1 (P24320) 30S RIBOSOMAL PROTEIN S15 × 1 (P80378) 30S RIBOSOMAL PROTEIN S16 × 1 30S RIBOSOMAL PROTEIN S17 × 1 30S RIBOSOMAL PROTEIN S18 × 1 30S RIBOSOMAL PROTEIN S19 × 1 (P80381) 30S RIBOSOMAL PROTEIN S20 × 1 30S RIBOSOMAL PROTEIN THX × 1 (P32193) MG MAGNESIUM ION × 96 PCY Pactamycin × 1 ZN ZINC ION × 2 X-RAY DIFFRACTION X-ray crystallization conditions:VAPOR DIFFUSION, HANGING DROP;pH 6.5;277 K;VAPOR DIFFUSION, HANGING DROP AT 277 K. MPD, NH4CL, KCL, CACL2, MAGNESIUM ACETATE, SODIUM CACODYLATE, pH 6.50. 80 uM Pactamycin soaked into preformed crystals. Resolution 3.40 Å R-free 0.280

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

51 other PDB entries and 64 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name RS6_THETH
Isoform
PDB entities 7
Chains and sequence ranges Author chain F; PDBConstruct 1–101; UniProt 1–101

30S RIBOSOMAL PROTEIN S7

OrganismNot specified

UniProt P17291

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein–RNA Heteromer Protein × 20 RNA 2 PDB declaration: 22-meric(22) Consistent with all polymer counts Chain G; UniProt 1–156 Not recorded 16S RIBOSOMAL RNA × 1 FRAGMENT OF MESSENGER RNA × 1 30S RIBOSOMAL PROTEIN S2 × 1 30S RIBOSOMAL PROTEIN S3 × 1 30S RIBOSOMAL PROTEIN S4 × 1 (P80373) 30S RIBOSOMAL PROTEIN S5 × 1 (P27152) 30S RIBOSOMAL PROTEIN S6 × 1 (P23370) 30S RIBOSOMAL PROTEIN S8 × 1 (P24319) 30S RIBOSOMAL PROTEIN S9 × 1 30S RIBOSOMAL PROTEIN S10 × 1 (P80375) 30S RIBOSOMAL PROTEIN S11 × 1 30S RIBOSOMAL PROTEIN S12 × 1 (P17293) 30S RIBOSOMAL PROTEIN S13 × 1 30S RIBOSOMAL PROTEIN S14 × 1 (P24320) 30S RIBOSOMAL PROTEIN S15 × 1 (P80378) 30S RIBOSOMAL PROTEIN S16 × 1 30S RIBOSOMAL PROTEIN S17 × 1 30S RIBOSOMAL PROTEIN S18 × 1 30S RIBOSOMAL PROTEIN S19 × 1 (P80381) 30S RIBOSOMAL PROTEIN S20 × 1 30S RIBOSOMAL PROTEIN THX × 1 (P32193) MG MAGNESIUM ION × 96 PCY Pactamycin × 1 ZN ZINC ION × 2 X-RAY DIFFRACTION X-ray crystallization conditions:VAPOR DIFFUSION, HANGING DROP;pH 6.5;277 K;VAPOR DIFFUSION, HANGING DROP AT 277 K. MPD, NH4CL, KCL, CACL2, MAGNESIUM ACETATE, SODIUM CACODYLATE, pH 6.50. 80 uM Pactamycin soaked into preformed crystals. Resolution 3.40 Å R-free 0.280

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

395 other PDB entries and 615 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name RS7_THETH
Isoform
PDB entities 8
Chains and sequence ranges Author chain G; PDBConstruct 1–156; UniProt 1–156

30S RIBOSOMAL PROTEIN S8

OrganismNot specified

UniProt P24319

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein–RNA Heteromer Protein × 20 RNA 2 PDB declaration: 22-meric(22) Consistent with all polymer counts Chain H; UniProt 1–138 Not recorded 16S RIBOSOMAL RNA × 1 FRAGMENT OF MESSENGER RNA × 1 30S RIBOSOMAL PROTEIN S2 × 1 30S RIBOSOMAL PROTEIN S3 × 1 30S RIBOSOMAL PROTEIN S4 × 1 (P80373) 30S RIBOSOMAL PROTEIN S5 × 1 (P27152) 30S RIBOSOMAL PROTEIN S6 × 1 (P23370) 30S RIBOSOMAL PROTEIN S7 × 1 (P17291) 30S RIBOSOMAL PROTEIN S9 × 1 30S RIBOSOMAL PROTEIN S10 × 1 (P80375) 30S RIBOSOMAL PROTEIN S11 × 1 30S RIBOSOMAL PROTEIN S12 × 1 (P17293) 30S RIBOSOMAL PROTEIN S13 × 1 30S RIBOSOMAL PROTEIN S14 × 1 (P24320) 30S RIBOSOMAL PROTEIN S15 × 1 (P80378) 30S RIBOSOMAL PROTEIN S16 × 1 30S RIBOSOMAL PROTEIN S17 × 1 30S RIBOSOMAL PROTEIN S18 × 1 30S RIBOSOMAL PROTEIN S19 × 1 (P80381) 30S RIBOSOMAL PROTEIN S20 × 1 30S RIBOSOMAL PROTEIN THX × 1 (P32193) MG MAGNESIUM ION × 96 PCY Pactamycin × 1 ZN ZINC ION × 2 X-RAY DIFFRACTION X-ray crystallization conditions:VAPOR DIFFUSION, HANGING DROP;pH 6.5;277 K;VAPOR DIFFUSION, HANGING DROP AT 277 K. MPD, NH4CL, KCL, CACL2, MAGNESIUM ACETATE, SODIUM CACODYLATE, pH 6.50. 80 uM Pactamycin soaked into preformed crystals. Resolution 3.40 Å R-free 0.280

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

36 other PDB entries and 45 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name RS8_THETH
Isoform
PDB entities 9
Chains and sequence ranges Author chain H; PDBConstruct 1–138; UniProt 1–138

30S RIBOSOMAL PROTEIN S10

OrganismNot specified

UniProt P80375

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein–RNA Heteromer Protein × 20 RNA 2 PDB declaration: 22-meric(22) Consistent with all polymer counts Chain J; UniProt 1–105 Not recorded 16S RIBOSOMAL RNA × 1 FRAGMENT OF MESSENGER RNA × 1 30S RIBOSOMAL PROTEIN S2 × 1 30S RIBOSOMAL PROTEIN S3 × 1 30S RIBOSOMAL PROTEIN S4 × 1 (P80373) 30S RIBOSOMAL PROTEIN S5 × 1 (P27152) 30S RIBOSOMAL PROTEIN S6 × 1 (P23370) 30S RIBOSOMAL PROTEIN S7 × 1 (P17291) 30S RIBOSOMAL PROTEIN S8 × 1 (P24319) 30S RIBOSOMAL PROTEIN S9 × 1 30S RIBOSOMAL PROTEIN S11 × 1 30S RIBOSOMAL PROTEIN S12 × 1 (P17293) 30S RIBOSOMAL PROTEIN S13 × 1 30S RIBOSOMAL PROTEIN S14 × 1 (P24320) 30S RIBOSOMAL PROTEIN S15 × 1 (P80378) 30S RIBOSOMAL PROTEIN S16 × 1 30S RIBOSOMAL PROTEIN S17 × 1 30S RIBOSOMAL PROTEIN S18 × 1 30S RIBOSOMAL PROTEIN S19 × 1 (P80381) 30S RIBOSOMAL PROTEIN S20 × 1 30S RIBOSOMAL PROTEIN THX × 1 (P32193) MG MAGNESIUM ION × 96 PCY Pactamycin × 1 ZN ZINC ION × 2 X-RAY DIFFRACTION X-ray crystallization conditions:VAPOR DIFFUSION, HANGING DROP;pH 6.5;277 K;VAPOR DIFFUSION, HANGING DROP AT 277 K. MPD, NH4CL, KCL, CACL2, MAGNESIUM ACETATE, SODIUM CACODYLATE, pH 6.50. 80 uM Pactamycin soaked into preformed crystals. Resolution 3.40 Å R-free 0.280

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

40 other PDB entries and 52 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name RS10_THETH
Isoform
PDB entities 11
Chains and sequence ranges Author chain J; PDBConstruct 1–105; UniProt 1–105

30S RIBOSOMAL PROTEIN S12

OrganismNot specified

UniProt P17293

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein–RNA Heteromer Protein × 20 RNA 2 PDB declaration: 22-meric(22) Consistent with all polymer counts Chain L; UniProt 1–135 Not recorded 16S RIBOSOMAL RNA × 1 FRAGMENT OF MESSENGER RNA × 1 30S RIBOSOMAL PROTEIN S2 × 1 30S RIBOSOMAL PROTEIN S3 × 1 30S RIBOSOMAL PROTEIN S4 × 1 (P80373) 30S RIBOSOMAL PROTEIN S5 × 1 (P27152) 30S RIBOSOMAL PROTEIN S6 × 1 (P23370) 30S RIBOSOMAL PROTEIN S7 × 1 (P17291) 30S RIBOSOMAL PROTEIN S8 × 1 (P24319) 30S RIBOSOMAL PROTEIN S9 × 1 30S RIBOSOMAL PROTEIN S10 × 1 (P80375) 30S RIBOSOMAL PROTEIN S11 × 1 30S RIBOSOMAL PROTEIN S13 × 1 30S RIBOSOMAL PROTEIN S14 × 1 (P24320) 30S RIBOSOMAL PROTEIN S15 × 1 (P80378) 30S RIBOSOMAL PROTEIN S16 × 1 30S RIBOSOMAL PROTEIN S17 × 1 30S RIBOSOMAL PROTEIN S18 × 1 30S RIBOSOMAL PROTEIN S19 × 1 (P80381) 30S RIBOSOMAL PROTEIN S20 × 1 30S RIBOSOMAL PROTEIN THX × 1 (P32193) MG MAGNESIUM ION × 96 PCY Pactamycin × 1 ZN ZINC ION × 2 X-RAY DIFFRACTION X-ray crystallization conditions:VAPOR DIFFUSION, HANGING DROP;pH 6.5;277 K;VAPOR DIFFUSION, HANGING DROP AT 277 K. MPD, NH4CL, KCL, CACL2, MAGNESIUM ACETATE, SODIUM CACODYLATE, pH 6.50. 80 uM Pactamycin soaked into preformed crystals. Resolution 3.40 Å R-free 0.280

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

35 other PDB entries and 47 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name RS12_THETH
Isoform
PDB entities 13
Chains and sequence ranges Author chain L; PDBConstruct 1–135; UniProt 1–135

30S RIBOSOMAL PROTEIN S14

OrganismNot specified

UniProt P24320

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein–RNA Heteromer Protein × 20 RNA 2 PDB declaration: 22-meric(22) Consistent with all polymer counts Chain N; UniProt 1–61 Not recorded 16S RIBOSOMAL RNA × 1 FRAGMENT OF MESSENGER RNA × 1 30S RIBOSOMAL PROTEIN S2 × 1 30S RIBOSOMAL PROTEIN S3 × 1 30S RIBOSOMAL PROTEIN S4 × 1 (P80373) 30S RIBOSOMAL PROTEIN S5 × 1 (P27152) 30S RIBOSOMAL PROTEIN S6 × 1 (P23370) 30S RIBOSOMAL PROTEIN S7 × 1 (P17291) 30S RIBOSOMAL PROTEIN S8 × 1 (P24319) 30S RIBOSOMAL PROTEIN S9 × 1 30S RIBOSOMAL PROTEIN S10 × 1 (P80375) 30S RIBOSOMAL PROTEIN S11 × 1 30S RIBOSOMAL PROTEIN S12 × 1 (P17293) 30S RIBOSOMAL PROTEIN S13 × 1 30S RIBOSOMAL PROTEIN S15 × 1 (P80378) 30S RIBOSOMAL PROTEIN S16 × 1 30S RIBOSOMAL PROTEIN S17 × 1 30S RIBOSOMAL PROTEIN S18 × 1 30S RIBOSOMAL PROTEIN S19 × 1 (P80381) 30S RIBOSOMAL PROTEIN S20 × 1 30S RIBOSOMAL PROTEIN THX × 1 (P32193) MG MAGNESIUM ION × 96 PCY Pactamycin × 1 ZN ZINC ION × 2 X-RAY DIFFRACTION X-ray crystallization conditions:VAPOR DIFFUSION, HANGING DROP;pH 6.5;277 K;VAPOR DIFFUSION, HANGING DROP AT 277 K. MPD, NH4CL, KCL, CACL2, MAGNESIUM ACETATE, SODIUM CACODYLATE, pH 6.50. 80 uM Pactamycin soaked into preformed crystals. Resolution 3.40 Å R-free 0.280

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

42 other PDB entries and 56 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name RS14_THETH
Isoform
PDB entities 15
Chains and sequence ranges Author chain N; PDBConstruct 1–61; UniProt 1–61

30S RIBOSOMAL PROTEIN S15

OrganismNot specified

UniProt P80378

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein–RNA Heteromer Protein × 20 RNA 2 PDB declaration: 22-meric(22) Consistent with all polymer counts Chain O; UniProt 1–89 Not recorded 16S RIBOSOMAL RNA × 1 FRAGMENT OF MESSENGER RNA × 1 30S RIBOSOMAL PROTEIN S2 × 1 30S RIBOSOMAL PROTEIN S3 × 1 30S RIBOSOMAL PROTEIN S4 × 1 (P80373) 30S RIBOSOMAL PROTEIN S5 × 1 (P27152) 30S RIBOSOMAL PROTEIN S6 × 1 (P23370) 30S RIBOSOMAL PROTEIN S7 × 1 (P17291) 30S RIBOSOMAL PROTEIN S8 × 1 (P24319) 30S RIBOSOMAL PROTEIN S9 × 1 30S RIBOSOMAL PROTEIN S10 × 1 (P80375) 30S RIBOSOMAL PROTEIN S11 × 1 30S RIBOSOMAL PROTEIN S12 × 1 (P17293) 30S RIBOSOMAL PROTEIN S13 × 1 30S RIBOSOMAL PROTEIN S14 × 1 (P24320) 30S RIBOSOMAL PROTEIN S16 × 1 30S RIBOSOMAL PROTEIN S17 × 1 30S RIBOSOMAL PROTEIN S18 × 1 30S RIBOSOMAL PROTEIN S19 × 1 (P80381) 30S RIBOSOMAL PROTEIN S20 × 1 30S RIBOSOMAL PROTEIN THX × 1 (P32193) MG MAGNESIUM ION × 96 PCY Pactamycin × 1 ZN ZINC ION × 2 X-RAY DIFFRACTION X-ray crystallization conditions:VAPOR DIFFUSION, HANGING DROP;pH 6.5;277 K;VAPOR DIFFUSION, HANGING DROP AT 277 K. MPD, NH4CL, KCL, CACL2, MAGNESIUM ACETATE, SODIUM CACODYLATE, pH 6.50. 80 uM Pactamycin soaked into preformed crystals. Resolution 3.40 Å R-free 0.280

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

47 other PDB entries and 59 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name RS15_THETH
Isoform
PDB entities 16
Chains and sequence ranges Author chain O; PDBConstruct 1–89; UniProt 1–89

30S RIBOSOMAL PROTEIN S19

OrganismNot specified

UniProt P80381

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein–RNA Heteromer Protein × 20 RNA 2 PDB declaration: 22-meric(22) Consistent with all polymer counts Chain S; UniProt 1–93 Not recorded 16S RIBOSOMAL RNA × 1 FRAGMENT OF MESSENGER RNA × 1 30S RIBOSOMAL PROTEIN S2 × 1 30S RIBOSOMAL PROTEIN S3 × 1 30S RIBOSOMAL PROTEIN S4 × 1 (P80373) 30S RIBOSOMAL PROTEIN S5 × 1 (P27152) 30S RIBOSOMAL PROTEIN S6 × 1 (P23370) 30S RIBOSOMAL PROTEIN S7 × 1 (P17291) 30S RIBOSOMAL PROTEIN S8 × 1 (P24319) 30S RIBOSOMAL PROTEIN S9 × 1 30S RIBOSOMAL PROTEIN S10 × 1 (P80375) 30S RIBOSOMAL PROTEIN S11 × 1 30S RIBOSOMAL PROTEIN S12 × 1 (P17293) 30S RIBOSOMAL PROTEIN S13 × 1 30S RIBOSOMAL PROTEIN S14 × 1 (P24320) 30S RIBOSOMAL PROTEIN S15 × 1 (P80378) 30S RIBOSOMAL PROTEIN S16 × 1 30S RIBOSOMAL PROTEIN S17 × 1 30S RIBOSOMAL PROTEIN S18 × 1 30S RIBOSOMAL PROTEIN S20 × 1 30S RIBOSOMAL PROTEIN THX × 1 (P32193) MG MAGNESIUM ION × 96 PCY Pactamycin × 1 ZN ZINC ION × 2 X-RAY DIFFRACTION X-ray crystallization conditions:VAPOR DIFFUSION, HANGING DROP;pH 6.5;277 K;VAPOR DIFFUSION, HANGING DROP AT 277 K. MPD, NH4CL, KCL, CACL2, MAGNESIUM ACETATE, SODIUM CACODYLATE, pH 6.50. 80 uM Pactamycin soaked into preformed crystals. Resolution 3.40 Å R-free 0.280

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

45 other PDB entries and 55 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name RS19_THETH
Isoform
PDB entities 20
Chains and sequence ranges Author chain S; PDBConstruct 1–93; UniProt 1–93

30S RIBOSOMAL PROTEIN THX

OrganismNot specified

UniProt P32193

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein–RNA Heteromer Protein × 20 RNA 2 PDB declaration: 22-meric(22) Consistent with all polymer counts Chain V; UniProt 2–26 Not recorded 16S RIBOSOMAL RNA × 1 FRAGMENT OF MESSENGER RNA × 1 30S RIBOSOMAL PROTEIN S2 × 1 30S RIBOSOMAL PROTEIN S3 × 1 30S RIBOSOMAL PROTEIN S4 × 1 (P80373) 30S RIBOSOMAL PROTEIN S5 × 1 (P27152) 30S RIBOSOMAL PROTEIN S6 × 1 (P23370) 30S RIBOSOMAL PROTEIN S7 × 1 (P17291) 30S RIBOSOMAL PROTEIN S8 × 1 (P24319) 30S RIBOSOMAL PROTEIN S9 × 1 30S RIBOSOMAL PROTEIN S10 × 1 (P80375) 30S RIBOSOMAL PROTEIN S11 × 1 30S RIBOSOMAL PROTEIN S12 × 1 (P17293) 30S RIBOSOMAL PROTEIN S13 × 1 30S RIBOSOMAL PROTEIN S14 × 1 (P24320) 30S RIBOSOMAL PROTEIN S15 × 1 (P80378) 30S RIBOSOMAL PROTEIN S16 × 1 30S RIBOSOMAL PROTEIN S17 × 1 30S RIBOSOMAL PROTEIN S18 × 1 30S RIBOSOMAL PROTEIN S19 × 1 (P80381) 30S RIBOSOMAL PROTEIN S20 × 1 MG MAGNESIUM ION × 96 PCY Pactamycin × 1 ZN ZINC ION × 2 X-RAY DIFFRACTION X-ray crystallization conditions:VAPOR DIFFUSION, HANGING DROP;pH 6.5;277 K;VAPOR DIFFUSION, HANGING DROP AT 277 K. MPD, NH4CL, KCL, CACL2, MAGNESIUM ACETATE, SODIUM CACODYLATE, pH 6.50. 80 uM Pactamycin soaked into preformed crystals. Resolution 3.40 Å R-free 0.280

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

16 other PDB entries and 16 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name RSHX_THETH
Isoform
PDB entities 22
Chains and sequence ranges Author chain V; PDBConstruct 1–25; UniProt 2–26

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

SAXS scattering curve SAXS Profile

SAXS profile for 1hnx

P(r) Distance Distribution P(r) Distribution

P(r) distribution for 1hnx
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2. Structure Basics 2. Structure Basics

Entry ID entry_id1hnx
Deposition date deposition_date2000-12-08
Structure title titleSTRUCTURE OF THE THERMUS THERMOPHILUS 30S RIBOSOMAL SUBUNIT IN COMPLEX WITH PACTAMYCIN
Keywords keywords30S RIBOSOMAL SUBUNIT, RIBOSOME, ANTIBIOTIC, PACTAMYCIN; RIBOSOME
Experimental Method methodX-RAY DIFFRACTION

3. SAXS Parameters (CRYSOL theoretical calculation) 3. SAXS Parameters (CRYSOL)

Radius of gyration Rg (Guinier) rg_guinier65.87
Radius of gyration Rg (electron density) rg_electron66.36
Forward intensity I(0) i018397300000.00
Molecular weight molecular_weight769240.0 kDa
Excluded volume excluded_volume805660 ų
Envelope volume envelope_volume1286900 ų
Hydration-shell volume shell_volume159860 ų
Envelope diameter envelope_diameter241.4
Shell Rg shell_rg67.59
Envelope Rg envelope_rg65.52
Shape Rg shape_rg66.35
Total Rg total_rg66.39
Total atoms total_atoms51910
Residues n_residues3914
Spherical-harmonic order n_harmonics20
q range q_range— – 0.5000 −1
Data points n_points101
Shell type shell_typedirectional
Solvent electron density solvent_density0.3340 e/ų
Shell contrast contrast_shell0.0300 e/ų
CRYSOL version crysol_version4.1.3

4. P(r) Distance Distribution (GNOM inversion) 4. P(r) Analysis (GNOM)

Maximum dimension Dmax dmax234.1
Rg (real space) rg_real66.08
Rg uncertainty (real space) rg_real_error2.06
I(0) (real space) i0_real1.8400e+10
I(0) uncertainty (real space) i0_real_error3.6160e+08
Rg (reciprocal space) rg_reciprocal65.65
I(0) (reciprocal space) i0_reciprocal18380000000.0000
Solution quality estimate total_estimate0.8475
Solution quality rating solution_quality GOOD a GOOD solution
P(r) peaks n_peaks1
Primary peak position r_peak_primary68.3
Skewness Skewness skewness0.421
Kurtosis Kurtosis kurtosis-0.303
Angular range angular_range— – 0.1200 −1
Current regularization parameter α current_alpha0.0003
Highest regularization parameter α highest_alpha1258000000.0000
Real-space data points n_real_points25
GNOM version gnom_version4.1.3
Quality Criteria quality_criteria AN1: 0.000; Oscil: 0.774; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.999; Smooth: 0.691

5. Crystallography and Experiment 5. Crystallography & Experiment

6. Entities and Polymers Entities & Polymers (25)

7. Fold Classification (SCOP + CATH) 47 domains

SCOP 2.08 (22 domains)

Domain ID domain_idd1hnxb_
Class classc — Alpha and beta proteins (a/b)
Fold Fold foldc.23 — Flavodoxin-like
Superfamily Superfamily superfamilyc.23.15 — Ribosomal protein S2
Family Family familyc.23.15.1 — Ribosomal protein S2
Domain ID domain_idd1hnxc1
Class classd — Alpha and beta proteins (a+b)
Fold Fold foldd.52 — Alpha-lytic protease prodomain-like
Superfamily Superfamily superfamilyd.52.3 — Prokaryotic type KH domain (KH-domain type II)
Family Family familyd.52.3.1 — Prokaryotic type KH domain (KH-domain type II)
Domain ID domain_idd1hnxc2
Class classd — Alpha and beta proteins (a+b)
Fold Fold foldd.53 — Ribosomal protein S3 C-terminal domain
Superfamily Superfamily superfamilyd.53.1 — Ribosomal protein S3 C-terminal domain
Family Family familyd.53.1.1 — Ribosomal protein S3 C-terminal domain
Domain ID domain_idd1hnxd_
Class classd — Alpha and beta proteins (a+b)
Fold Fold foldd.66 — Alpha-L RNA-binding motif
Superfamily Superfamily superfamilyd.66.1 — Alpha-L RNA-binding motif
Family Family familyd.66.1.2 — Ribosomal protein S4 (bacteria)
Domain ID domain_idd1hnxe1
Class classd — Alpha and beta proteins (a+b)
Fold Fold foldd.14 — Ribosomal protein S5 domain 2-like
Superfamily Superfamily superfamilyd.14.1 — Ribosomal protein S5 domain 2-like
Family Family familyd.14.1.1 — Translational machinery components
Domain ID domain_idd1hnxe2
Class classd — Alpha and beta proteins (a+b)
Fold Fold foldd.50 — dsRBD-like
Superfamily Superfamily superfamilyd.50.1 — dsRNA-binding domain-like
Family Family familyd.50.1.2 — Ribosomal S5 protein, N-terminal domain
Domain ID domain_idd1hnxf_
Class classd — Alpha and beta proteins (a+b)
Fold Fold foldd.58 — Ferredoxin-like
Superfamily Superfamily superfamilyd.58.14 — Ribosomal protein S6
Family Family familyd.58.14.1 — Ribosomal protein S6
Domain ID domain_idd1hnxg_
Class classa — All alpha proteins
Fold Fold folda.75 — Ribosomal protein S7
Superfamily Superfamily superfamilya.75.1 — Ribosomal protein S7
Family Family familya.75.1.1 — Ribosomal protein S7
Domain ID domain_idd1hnxh_
Class classd — Alpha and beta proteins (a+b)
Fold Fold foldd.140 — Ribosomal protein S8
Superfamily Superfamily superfamilyd.140.1 — Ribosomal protein S8
Family Family familyd.140.1.1 — Ribosomal protein S8
Domain ID domain_idd1hnxi_
Class classd — Alpha and beta proteins (a+b)
Fold Fold foldd.14 — Ribosomal protein S5 domain 2-like
Superfamily Superfamily superfamilyd.14.1 — Ribosomal protein S5 domain 2-like
Family Family familyd.14.1.1 — Translational machinery components
Domain ID domain_idd1hnxj_
Class classd — Alpha and beta proteins (a+b)
Fold Fold foldd.58 — Ferredoxin-like
Superfamily Superfamily superfamilyd.58.15 — Ribosomal protein S10
Family Family familyd.58.15.1 — Ribosomal protein S10
Domain ID domain_idd1hnxk_
Class classc — Alpha and beta proteins (a/b)
Fold Fold foldc.55 — Ribonuclease H-like motif
Superfamily Superfamily superfamilyc.55.4 — Translational machinery components
Family Family familyc.55.4.1 — Ribosomal protein L18 and S11
Domain ID domain_idd1hnxl_
Class classb — All beta proteins
Fold Fold foldb.40 — OB-fold
Superfamily Superfamily superfamilyb.40.4 — Nucleic acid-binding proteins
Family Family familyb.40.4.5 — Cold shock DNA-binding domain-like
Domain ID domain_idd1hnxm_
Class classa — All alpha proteins
Fold Fold folda.156 — S13-like H2TH domain
Superfamily Superfamily superfamilya.156.1 — S13-like H2TH domain
Family Family familya.156.1.1 — Ribosomal protein S13
Domain ID domain_idd1hnxn_
Class classg — Small proteins
Fold Fold foldg.39 — Glucocorticoid receptor-like (DNA-binding domain)
Superfamily Superfamily superfamilyg.39.1 — Glucocorticoid receptor-like (DNA-binding domain)
Family Family familyg.39.1.7 — Ribosomal protein S14
Domain ID domain_idd1hnxo_
Class classa — All alpha proteins
Fold Fold folda.16 — S15/NS1 RNA-binding domain
Superfamily Superfamily superfamilya.16.1 — S15/NS1 RNA-binding domain
Family Family familya.16.1.2 — Ribosomal protein S15
Domain ID domain_idd1hnxp_
Class classd — Alpha and beta proteins (a+b)
Fold Fold foldd.27 — Ribosomal protein S16
Superfamily Superfamily superfamilyd.27.1 — Ribosomal protein S16
Family Family familyd.27.1.1 — Ribosomal protein S16
Domain ID domain_idd1hnxq_
Class classb — All beta proteins
Fold Fold foldb.40 — OB-fold
Superfamily Superfamily superfamilyb.40.4 — Nucleic acid-binding proteins
Family Family familyb.40.4.5 — Cold shock DNA-binding domain-like
Domain ID domain_idd1hnxr_
Class classa — All alpha proteins
Fold Fold folda.4 — DNA/RNA-binding 3-helical bundle
Superfamily Superfamily superfamilya.4.8 — Ribosomal protein S18
Family Family familya.4.8.1 — Ribosomal protein S18
Domain ID domain_idd1hnxs_
Class classd — Alpha and beta proteins (a+b)
Fold Fold foldd.28 — Ribosomal protein S19
Superfamily Superfamily superfamilyd.28.1 — Ribosomal protein S19
Family Family familyd.28.1.1 — Ribosomal protein S19
Domain ID domain_idd1hnxt_
Class classa — All alpha proteins
Fold Fold folda.7 — Spectrin repeat-like
Superfamily Superfamily superfamilya.7.6 — Ribosomal protein S20
Family Family familya.7.6.1 — Ribosomal protein S20
Domain ID domain_idd1hnxv_
Class classj — Peptides
Fold Fold foldj.9 — Arg-rich RNA binding peptides
Superfamily Superfamily superfamilyj.9.1 — 30S ribosomal protein THX
Family Family familyj.9.1.1 — 30S ribosomal protein THX

CATH v4.4 (25 domains)

Domain ID domain_id1hnxB01
Class class3 — Alpha Beta
Architecture architecture40 — 3-Layer(aba) Sandwich
Topology topology50 — Rossmann fold
Homologous superfamily homologous superfamily10490 — Glucose-6-phosphate isomerase like protein; domain 1
Domain ID domain_id1hnxB02
Class class1 — Mainly Alpha
Architecture architecture10 — Orthogonal Bundle
Topology topology287 — Helix Hairpins
Homologous superfamily homologous superfamily610 — Helix hairpin bin
Domain ID domain_id1hnxC01
Class class3 — Alpha Beta
Architecture architecture30 — 2-Layer Sandwich
Topology topology300 — GMP Synthetase; Chain A, domain 3
Homologous superfamily homologous superfamily20 — K homology (KH) domain
Domain ID domain_id1hnxC02
Class class3 — Alpha Beta
Architecture architecture30 — 2-Layer Sandwich
Topology topology1140 — Ribosomal protein S3 C-terminal domain
Homologous superfamily homologous superfamily32 — Ribosomal protein S3, C-terminal domain
Domain ID domain_id1hnxD01
Class class1 — Mainly Alpha
Architecture architecture10 — Orthogonal Bundle
Topology topology1050 — Ribosomal Protein S4 Delta 41; Chain A, domain 1
Homologous superfamily homologous superfamily10 — Ribosomal protein S4/S9, N-terminal domain
Domain ID domain_id1hnxD02
Class class3 — Alpha Beta
Architecture architecture10 — Roll
Topology topology290 — Structural Genomics Hypothetical 15.5 Kd Protein In mrcA-pckA Intergenic Region; Chain A
Homologous superfamily homologous superfamily10 — RNA-binding S4 domain
Domain ID domain_id1hnxE01
Class class3 — Alpha Beta
Architecture architecture30 — 2-Layer Sandwich
Topology topology160 — Double Stranded RNA Binding Domain
Homologous superfamily homologous superfamily20
Domain ID domain_id1hnxE02
Class class3 — Alpha Beta
Architecture architecture30 — 2-Layer Sandwich
Topology topology230 — Ribosomal Protein S5; domain 2
Homologous superfamily homologous superfamily10
Domain ID domain_id1hnxF00
Class class3 — Alpha Beta
Architecture architecture30 — 2-Layer Sandwich
Topology topology70 — Alpha-Beta Plaits
Homologous superfamily homologous superfamily60 — Ribosomal protein S6/Translation elongation factor EF1B
Domain ID domain_id1hnxG00
Class class1 — Mainly Alpha
Architecture architecture10 — Orthogonal Bundle
Topology topology455 — Ribosomal Protein S7
Homologous superfamily homologous superfamily10 — Ribosomal protein S7/S5
Domain ID domain_id1hnxH01
Class class3 — Alpha Beta
Architecture architecture30 — 2-Layer Sandwich
Topology topology1370 — Ribosomal Protein S8; Chain: A, domain 1
Homologous superfamily homologous superfamily30
Domain ID domain_id1hnxH02
Class class3 — Alpha Beta
Architecture architecture30 — 2-Layer Sandwich
Topology topology1490 — Dna Ligase; domain 1
Homologous superfamily homologous superfamily10
Domain ID domain_id1hnxI00
Class class3 — Alpha Beta
Architecture architecture30 — 2-Layer Sandwich
Topology topology230 — Ribosomal Protein S5; domain 2
Homologous superfamily homologous superfamily10
Domain ID domain_id1hnxJ00
Class class3 — Alpha Beta
Architecture architecture30 — 2-Layer Sandwich
Topology topology70 — Alpha-Beta Plaits
Homologous superfamily homologous superfamily600 — Ribosomal protein S10
Domain ID domain_id1hnxK00
Class class3 — Alpha Beta
Architecture architecture30 — 2-Layer Sandwich
Topology topology420 — Nucleotidyltransferase; domain 5
Homologous superfamily homologous superfamily80 — Ribosomal protein S11/S14
Domain ID domain_id1hnxL00
Class class2 — Mainly Beta
Architecture architecture40 — Beta Barrel
Topology topology50 — OB fold (Dihydrolipoamide Acetyltransferase, E2P)
Homologous superfamily homologous superfamily140 — Nucleic acid-binding proteins
Domain ID domain_id1hnxM01
Class class1 — Mainly Alpha
Architecture architecture10 — Orthogonal Bundle
Topology topology8 — Helicase, Ruva Protein; domain 3
Homologous superfamily homologous superfamily50
Domain ID domain_id1hnxM02
Class class4 — Few Secondary Structures
Architecture architecture10 — Irregular
Topology topology910 — 30s ribosomal protein s13; domain 2
Homologous superfamily homologous superfamily10 — Ribosomal protein S13/S18, C-terminal domain
Domain ID domain_id1hnxN00
Class class4 — Few Secondary Structures
Architecture architecture10 — Irregular
Topology topology830 — 30s Ribosomal Protein S14; Chain N
Homologous superfamily homologous superfamily10 — Ribosomal Protein S14/S29
Domain ID domain_id1hnxO00
Class class1 — Mainly Alpha
Architecture architecture10 — Orthogonal Bundle
Topology topology287 — Helix Hairpins
Homologous superfamily homologous superfamily10 — S15/NS1, RNA-binding
Domain ID domain_id1hnxP00
Class class3 — Alpha Beta
Architecture architecture30 — 2-Layer Sandwich
Topology topology1320 — S16 Ribosomal Protein; Chain: A;
Homologous superfamily homologous superfamily10 — Ribosomal protein S16
Domain ID domain_id1hnxQ00
Class class2 — Mainly Beta
Architecture architecture40 — Beta Barrel
Topology topology50 — OB fold (Dihydrolipoamide Acetyltransferase, E2P)
Homologous superfamily homologous superfamily140 — Nucleic acid-binding proteins
Domain ID domain_id1hnxR00
Class class4 — Few Secondary Structures
Architecture architecture10 — Irregular
Topology topology640 — 30s Ribosomal Protein S18
Homologous superfamily homologous superfamily10 — Ribosomal protein S18
Domain ID domain_id1hnxS00
Class class3 — Alpha Beta
Architecture architecture30 — 2-Layer Sandwich
Topology topology860 — 30s Ribosomal Protein S19; Chain A
Homologous superfamily homologous superfamily10 — Ribosomal protein S19/S15
Domain ID domain_id1hnxT00
Class class1 — Mainly Alpha
Architecture architecture20 — Up-down Bundle
Topology topology58 — Methane Monooxygenase Hydroxylase; Chain G, domain 1
Homologous superfamily homologous superfamily110 — Ribosomal protein S20

8. Citations (4)

9. Files and Curves (10)