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Protein Structures and Oligomeric States

Enter a protein name, UniProt accession or PDB ID. Name searches ask you to confirm the protein identity before related structures are queried.

Current Protein Identity：P82921 New Search
71PDB entries
71Biological assembly
71chain/assembly records
Main Difference Dimensions in This Set
Different construct Different mutation/modification Different assembly state Different ligand/ion Different experimental conditions Different structure-quality metrics

Difference tags compare only the current result set; every original PDB and assembly record remains separate.

Related-Structure Differences

Each row represents one biological assembly in one PDB entry; multiple monomers of the same protein are listed separately.

PDB Entry Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Experimental Method Experimental Conditions Structure Quality
3J9M Structure of the human mitochondrial ribosome (class 1) Deposited 2015-02-08 Assembly 1 Protein–RNA Heteromer；Protein × 81 PDB declaration: 85-meric（85） Consistent with all polymers
Chain AQ 1–87（87 aa）
Not recorded MG MAGNESIUM ION × 127 ZN ZINC ION × 7 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer 20 mM HEPES-KOH, pH 7.45, 100 mM KCl, 20 mM MgOAc, 2 mM DTT；pH 7.45；20 mM HEPES-KOH, pH 7.45, 100 mM KCl, 20 mM MgOAc, 2 mM DTT
cryo-EM vitrification conditions Blot 2.5 seconds before plunging；90 K；Cryogen ETHANE；Blot 2.5 seconds before plunging into liquid ethane (FEI VITROBOT MARK II).
Resolution 3.50 Å
6NU2 Structural insights into unique features of the human mitochondrial ribosome recycling Deposited 2019-01-30 Assembly 1 Protein–RNA Heteromer；Protein × 82 PDB declaration: 86-meric（86） Consistent with all polymers
Chain AQ 2–87（86 aa）
Not recorded MG MAGNESIUM ION × 127 ZN ZINC ION × 7 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.90 Å
6NU3 Structural insights into unique features of the human mitochondrial ribosome recycling Deposited 2019-01-30 Assembly 1 Protein–RNA Heteromer；Protein × 81 PDB declaration: 85-meric（85） Consistent with all polymers
Chain AQ 2–87（86 aa）
Not recorded MG MAGNESIUM ION × 127 ZN ZINC ION × 7 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.40 Å
6RW4 Structure of human mitochondrial 28S ribosome in complex with mitochondrial IF3 Deposited 2019-06-03 Assembly 1 Protein–RNA Heteromer；Protein × 31 PDB declaration: 32-meric（32） Consistent with all polymers
Chain Q 2–87（86 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) NAD NICOTINAMIDE-ADENINE-DINUCLEOTIDE × 1 SPM SPERMINE × 1 SRY STREPTOMYCIN × 1 MG MAGNESIUM ION × 60 K POTASSIUM ION × 17 ZN ZINC ION × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.97 Å
6RW5 Structure of human mitochondrial 28S ribosome in complex with mitochondrial IF2 and IF3 Deposited 2019-06-03 Assembly 1 Protein–RNA Heteromer；Protein × 32 PDB declaration: 33-meric（33） Consistent with all polymers
Chain Q 2–87（86 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) NAD NICOTINAMIDE-ADENINE-DINUCLEOTIDE × 1 SPM SPERMINE × 1 SRY STREPTOMYCIN × 1 MG MAGNESIUM ION × 61 K POTASSIUM ION × 17 ZN ZINC ION × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.14 Å
6VLZ Structure of the human mitochondrial ribosome-EF-G1 complex (ClassI) Deposited 2020-01-27 Assembly 1 Other combination Heteromer；Protein × 85 PDB declaration: 90-meric（90） Consistent with all polymers
Chain AQ 1–87（87 aa）
Not recorded MG MAGNESIUM ION × 130 ZN ZINC ION × 7 GDP GUANOSINE-5'-DIPHOSPHATE × 1 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.97 Å
6VMI Structure of the human mitochondrial ribosome-EF-G1 complex (ClassIII) Deposited 2020-01-28 Assembly 1 Other combination Heteromer；Protein × 85 PDB declaration: 91-meric（91） Consistent with all polymers
Chain AQ 1–87（87 aa）
Not recorded MG MAGNESIUM ION × 130 ZN ZINC ION × 7 GDP GUANOSINE-5'-DIPHOSPHATE × 1 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.96 Å
6ZM5 Human mitochondrial ribosome in complex with OXA1L, mRNA, A/A tRNA, P/P tRNA and nascent polypeptide Deposited 2020-07-01 Assembly 1 Protein–RNA Heteromer；Protein × 88 PDB declaration: 94-meric（94） Consistent with all polymers
Chain AQ 2–87（86 aa）
Mutation：Natural variant C50R Non-standard monomer：Yes (specific site not provided by mmCIF) MG MAGNESIUM ION × 189 K POTASSIUM ION × 44 ZN ZINC ION × 3 FES FE2/S2 (INORGANIC) CLUSTER × 3 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ALA ALANINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.89 Å
6ZM6 Human mitochondrial ribosome in complex with mRNA, A/A tRNA and P/P tRNA Deposited 2020-07-01 Assembly 1 Protein–RNA Heteromer；Protein × 86 PDB declaration: 92-meric（92） Consistent with all polymers
Chain AQ 2–87（86 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) MG MAGNESIUM ION × 189 K POTASSIUM ION × 44 ZN ZINC ION × 3 FES FE2/S2 (INORGANIC) CLUSTER × 3 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.59 Å
6ZS9 Human mitochondrial ribosome in complex with ribosome recycling factor Deposited 2020-07-15 Assembly 1 Protein–RNA Heteromer；Protein × 88 PDB declaration: 91-meric（91） Consistent with all polymers
Chain AQ 2–87（86 aa）
Not recorded ZN ZINC ION × 7 MG MAGNESIUM ION × 195 DOL 5-(2-DIETHYLAMINO-ETHANESULFONYL)-21-HYDROXY-10-ISOPROPYL-11,19-DIMETHYL-9,26-DIOXA-3,15,28-TRIAZA-TRICYCLO[23.2.1.00,255]OCTACOSA-1(27),12,17,19,25(28)-PENTAENE-2,8,14,23-TETRAONE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.00 Å
6ZSA Human mitochondrial ribosome bound to mRNA, A-site tRNA and P-site tRNA Deposited 2020-07-15 Assembly 1 Protein–RNA Heteromer；Protein × 86 PDB declaration: 92-meric（92） Consistent with all polymers
Chain AQ 1–87（87 aa）
Not recorded ZN ZINC ION × 7 MG MAGNESIUM ION × 195 H8Q ~{N}-[(3~{S},6~{R},12~{R},15~{S},16~{S},19~{S},22~{S},25~{S})-25-[[(3~{S})-1-azabicyclo[2.2.2]octan-3-yl]sulfanylmethyl]-3-[[4-(dimethylamino)phenyl]methyl]-12-ethyl-4,16-dimethyl-2,5,11,14,18,21,24-heptakis(oxidanylidene)-19-phenyl-17-oxa-1,4,10,13,20-pentazatricyclo[20.4.0.0^{6,10}]hexacosan-15-yl]-3-oxidanyl-pyridine-2-carboxamide × 1 DOL 5-(2-DIETHYLAMINO-ETHANESULFONYL)-21-HYDROXY-10-ISOPROPYL-11,19-DIMETHYL-9,26-DIOXA-3,15,28-TRIAZA-TRICYCLO[23.2.1.00,255]OCTACOSA-1(27),12,17,19,25(28)-PENTAENE-2,8,14,23-TETRAONE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.00 Å
6ZSB Human mitochondrial ribosome in complex with mRNA and P-site tRNA Deposited 2020-07-15 Assembly 1 Protein–RNA Heteromer；Protein × 87 PDB declaration: 92-meric（92） Consistent with all polymers
Chain AQ 1–87（87 aa）
Not recorded ZN ZINC ION × 7 MG MAGNESIUM ION × 195 DOL 5-(2-DIETHYLAMINO-ETHANESULFONYL)-21-HYDROXY-10-ISOPROPYL-11,19-DIMETHYL-9,26-DIOXA-3,15,28-TRIAZA-TRICYCLO[23.2.1.00,255]OCTACOSA-1(27),12,17,19,25(28)-PENTAENE-2,8,14,23-TETRAONE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.50 Å
6ZSC Human mitochondrial ribosome in complex with E-site tRNA Deposited 2020-07-15 Assembly 1 Protein–RNA Heteromer；Protein × 87 PDB declaration: 91-meric（91） Consistent with all polymers
Chain AQ 2–86（85 aa）
Not recorded ZN ZINC ION × 7 MG MAGNESIUM ION × 195 DOL 5-(2-DIETHYLAMINO-ETHANESULFONYL)-21-HYDROXY-10-ISOPROPYL-11,19-DIMETHYL-9,26-DIOXA-3,15,28-TRIAZA-TRICYCLO[23.2.1.00,255]OCTACOSA-1(27),12,17,19,25(28)-PENTAENE-2,8,14,23-TETRAONE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.50 Å
6ZSD Human mitochondrial ribosome in complex with mRNA, P-site tRNA and E-site tRNA Deposited 2020-07-15 Assembly 1 Protein–RNA Heteromer；Protein × 87 PDB declaration: 93-meric（93） Consistent with all polymers
Chain AQ 1–87（87 aa）
Not recorded ZN ZINC ION × 7 MG MAGNESIUM ION × 195 DOL 5-(2-DIETHYLAMINO-ETHANESULFONYL)-21-HYDROXY-10-ISOPROPYL-11,19-DIMETHYL-9,26-DIOXA-3,15,28-TRIAZA-TRICYCLO[23.2.1.00,255]OCTACOSA-1(27),12,17,19,25(28)-PENTAENE-2,8,14,23-TETRAONE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.70 Å
6ZSE Human mitochondrial ribosome in complex with mRNA, A/P-tRNA and P/E-tRNA Deposited 2020-07-15 Assembly 1 Protein–RNA Heteromer；Protein × 87 PDB declaration: 93-meric（93） Consistent with all polymers
Chain AQ 1–87（87 aa）
Not recorded ZN ZINC ION × 7 MG MAGNESIUM ION × 195 DOL 5-(2-DIETHYLAMINO-ETHANESULFONYL)-21-HYDROXY-10-ISOPROPYL-11,19-DIMETHYL-9,26-DIOXA-3,15,28-TRIAZA-TRICYCLO[23.2.1.00,255]OCTACOSA-1(27),12,17,19,25(28)-PENTAENE-2,8,14,23-TETRAONE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 5.00 Å
6ZSG Human mitochondrial ribosome in complex with mRNA, A-site tRNA, P-site tRNA and E-site tRNA Deposited 2020-07-15 Assembly 1 Protein–RNA Heteromer；Protein × 87 PDB declaration: 94-meric（94） Consistent with all polymers
Chain AQ 2–87（86 aa）
Not recorded ZN ZINC ION × 7 MG MAGNESIUM ION × 195 DOL 5-(2-DIETHYLAMINO-ETHANESULFONYL)-21-HYDROXY-10-ISOPROPYL-11,19-DIMETHYL-9,26-DIOXA-3,15,28-TRIAZA-TRICYCLO[23.2.1.00,255]OCTACOSA-1(27),12,17,19,25(28)-PENTAENE-2,8,14,23-TETRAONE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.00 Å
7A5F Structure of the stalled human mitoribosome with P- and E-site mt-tRNAs Deposited 2020-08-21 Assembly 1 Protein–RNA Heteromer；Protein × 85 PDB declaration: 91-meric（91） Consistent with all polymers
Chain Q6 1–87（87 aa）
Not recorded MG MAGNESIUM ION × 128 ZN ZINC ION × 7 GDP GUANOSINE-5'-DIPHOSPHATE × 1 SO4 SULFATE ION × 1 MLI MALONATE ION × 3 CL CHLORIDE ION × 2 NA SODIUM ION × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.40 Å
7A5G Structure of the elongating human mitoribosome bound to mtEF-Tu.GMPPCP and A/T mt-tRNA Deposited 2020-08-21 Assembly 1 Protein–RNA Heteromer；Protein × 86 PDB declaration: 94-meric（94） Consistent with all polymers
Chain Q6 1–87（87 aa）
Not recorded MG MAGNESIUM ION × 128 SPD SPERMIDINE × 1 ZN ZINC ION × 7 GDP GUANOSINE-5'-DIPHOSPHATE × 1 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 NA SODIUM ION × 4 SO4 SULFATE ION × 1 MLI MALONATE ION × 3 CL CHLORIDE ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.33 Å
7A5I Structure of the human mitoribosome with A- P-and E-site mt-tRNAs Deposited 2020-08-21 Assembly 1 Protein–RNA Heteromer；Protein × 83 PDB declaration: 90-meric（90） Consistent with all polymers
Chain Q6 1–87（87 aa）
Not recorded MG MAGNESIUM ION × 127 ZN ZINC ION × 7 CL CHLORIDE ION × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.70 Å
7A5K Structure of the human mitoribosome in the post translocation state bound to mtEF-G1 Deposited 2020-08-21 Assembly 1 Protein–RNA Heteromer；Protein × 86 PDB declaration: 93-meric（93） Consistent with all polymers
Chain Q6 1–87（87 aa）
Not recorded GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 MG MAGNESIUM ION × 128 SPD SPERMIDINE × 1 ZN ZINC ION × 7 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.70 Å
7L08 Cryo-EM structure of the human 55S mitoribosome-RRFmt complex. Deposited 2020-12-11 Assembly 1 Protein–RNA Heteromer；Protein × 84 PDB declaration: 87-meric（87） Consistent with all polymers
Chain AQ 1–87（87 aa）
Not recorded MG MAGNESIUM ION × 127 ZN ZINC ION × 7 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.49 Å
7OG4 Human mitochondrial ribosome in complex with P/E-tRNA Deposited 2021-05-06 Assembly 1 Protein–RNA Heteromer；Protein × 86 PDB declaration: 90-meric（90） Consistent with all polymers
Chain AQ 1–87（87 aa）
Not recorded ZN ZINC ION × 7 MG MAGNESIUM ION × 195 H8Q ~{N}-[(3~{S},6~{R},12~{R},15~{S},16~{S},19~{S},22~{S},25~{S})-25-[[(3~{S})-1-azabicyclo[2.2.2]octan-3-yl]sulfanylmethyl]-3-[[4-(dimethylamino)phenyl]methyl]-12-ethyl-4,16-dimethyl-2,5,11,14,18,21,24-heptakis(oxidanylidene)-19-phenyl-17-oxa-1,4,10,13,20-pentazatricyclo[20.4.0.0^{6,10}]hexacosan-15-yl]-3-oxidanyl-pyridine-2-carboxamide × 1 DOL 5-(2-DIETHYLAMINO-ETHANESULFONYL)-21-HYDROXY-10-ISOPROPYL-11,19-DIMETHYL-9,26-DIOXA-3,15,28-TRIAZA-TRICYCLO[23.2.1.00,255]OCTACOSA-1(27),12,17,19,25(28)-PENTAENE-2,8,14,23-TETRAONE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.80 Å
7P2E Human mitochondrial ribosome small subunit in complex with IF3, GMPPMP and streptomycin Deposited 2021-07-05 Assembly 1 Protein–RNA Heteromer；Protein × 31 PDB declaration: 32-meric（32） Consistent with all polymers
Chain Q 2–87（86 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) NAD NICOTINAMIDE-ADENINE-DINUCLEOTIDE × 1 SPM SPERMINE × 1 5I0 [(2~{S},3~{S},4~{S},5~{R},6~{S})-2-[(2~{R},3~{R},4~{R},5~{S})-2-[(1~{R},2~{S},3~{R},4~{R},5~{S},6~{R})-2,4-bis[[azaniumylidene(azanyl)methyl]amino]-3,5,6-tris(oxidanyl)cyclohexyl]oxy-4-[bis(oxidanyl)methyl]-5-methyl-4-oxidanyl-oxolan-3-yl]oxy-6-(hydroxymethyl)-4,5-bis(oxidanyl)oxan-3-yl]-methyl-azanium × 1 K POTASSIUM ION × 21 MG MAGNESIUM ION × 62 ZN ZINC ION × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.40 Å
7PNX Assembly intermediate of human mitochondrial ribosome small subunit without mS37 in complex with RBFA and METTL15 conformation a Deposited 2021-09-08 Assembly 1 Protein–RNA Heteromer；Protein × 31 PDB declaration: 32-meric（32） Consistent with all polymers
Chain Q 2–87（86 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) NAD NICOTINAMIDE-ADENINE-DINUCLEOTIDE × 1 MG MAGNESIUM ION × 64 K POTASSIUM ION × 16 ZN ZINC ION × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 SAH S-ADENOSYL-L-HOMOCYSTEINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.76 Å
7PNY Assembly intermediate of human mitochondrial ribosome small subunit without mS37 in complex with RBFA and METTL15 conformation b Deposited 2021-09-08 Assembly 1 Protein–RNA Heteromer；Protein × 31 PDB declaration: 32-meric（32） Consistent with all polymers
Chain Q 2–87（86 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) NAD NICOTINAMIDE-ADENINE-DINUCLEOTIDE × 1 MG MAGNESIUM ION × 61 K POTASSIUM ION × 20 ZN ZINC ION × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 SAH S-ADENOSYL-L-HOMOCYSTEINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.06 Å
7PNZ Assembly intermediate of human mitochondrial ribosome small subunit without mS37 in complex with RBFA and METTL15 conformation c Deposited 2021-09-08 Assembly 1 Protein–RNA Heteromer；Protein × 31 PDB declaration: 32-meric（32） Consistent with all polymers
Chain Q 2–87（86 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) NAD NICOTINAMIDE-ADENINE-DINUCLEOTIDE × 1 MG MAGNESIUM ION × 61 K POTASSIUM ION × 17 ZN ZINC ION × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 SAH S-ADENOSYL-L-HOMOCYSTEINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.09 Å
7PO0 Assembly intermediate of human mitochondrial ribosome small subunit without mS37 in complex with RBFA and IF3 Deposited 2021-09-08 Assembly 1 Protein–RNA Heteromer；Protein × 31 PDB declaration: 32-meric（32） Consistent with all polymers
Chain Q 2–87（86 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) NAD NICOTINAMIDE-ADENINE-DINUCLEOTIDE × 1 MG MAGNESIUM ION × 65 K POTASSIUM ION × 17 ZN ZINC ION × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
7PO1 Initiation complex of human mitochondrial ribosome small subunit with IF3 Deposited 2021-09-08 Assembly 1 Protein–RNA Heteromer；Protein × 31 PDB declaration: 32-meric（32） Consistent with all polymers
Chain Q 2–87（86 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) MG MAGNESIUM ION × 71 K POTASSIUM ION × 20 ZN ZINC ION × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.92 Å
7PO2 Initiation complex of human mitochondrial ribosome small subunit with IF2, fMet-tRNAMet and mRNA Deposited 2021-09-08 Assembly 1 Protein–RNA Heteromer；Protein × 32 PDB declaration: 35-meric（35） Consistent with all polymers
Chain Q 2–87（86 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) MG MAGNESIUM ION × 72 K POTASSIUM ION × 20 ZN ZINC ION × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 FME N-FORMYLMETHIONINE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.09 Å
7PO3 Human mitochondrial ribosome small subunit Deposited 2021-09-08 Assembly 1 Protein–RNA Heteromer；Protein × 30 PDB declaration: 31-meric（31） Consistent with all polymers
Chain Q 2–87（86 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) MG MAGNESIUM ION × 64 K POTASSIUM ION × 21 ZN ZINC ION × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.92 Å
8ANY Human mitochondrial ribosome in complex with LRPPRC, SLIRP, A-site, P-site, E-site tRNAs and mRNA Deposited 2022-08-06 Assembly 1 Protein–RNA Heteromer；Protein × 89 PDB declaration: 96-meric（96） Consistent with all polymers
Chain AQ 1–87（87 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) NAD NICOTINAMIDE-ADENINE-DINUCLEOTIDE × 1 SPM SPERMINE × 1 SPD SPERMIDINE × 4 MG MAGNESIUM ION × 206 K POTASSIUM ION × 49 ZN ZINC ION × 3 FES FE2/S2 (INORGANIC) CLUSTER × 3 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 PUT 1,4-DIAMINOBUTANE × 1 VAL VALINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.85 Å
8CSP Human mitochondrial small subunit assembly intermediate (State A) Deposited 2022-05-13 Assembly 1 Protein–RNA Heteromer；Protein × 32 PDB declaration: 33-meric（33） Consistent with all polymers
Chain Q 1–87（87 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) SF4 IRON/SULFUR CLUSTER × 1 SAM S-ADENOSYLMETHIONINE × 1 K POTASSIUM ION × 4 MG MAGNESIUM ION × 28 FES FE2/S2 (INORGANIC) CLUSTER × 2 ZN ZINC ION × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.66 Å
8CSQ Human mitochondrial small subunit assembly intermediate (State B) Deposited 2022-05-13 Assembly 1 Protein–RNA Heteromer；Protein × 32 PDB declaration: 33-meric（33） Consistent with all polymers
Chain Q 1–87（87 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) SF4 IRON/SULFUR CLUSTER × 1 SAM S-ADENOSYLMETHIONINE × 1 K POTASSIUM ION × 4 MG MAGNESIUM ION × 27 ZN ZINC ION × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.54 Å
8CSR Human mitochondrial small subunit assembly intermediate (State C) Deposited 2022-05-13 Assembly 1 Protein–RNA Heteromer；Protein × 33 PDB declaration: 34-meric（34） Consistent with all polymers
Chain Q 1–87（87 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) SF4 IRON/SULFUR CLUSTER × 1 SAM S-ADENOSYLMETHIONINE × 1 K POTASSIUM ION × 5 MG MAGNESIUM ION × 31 NAD NICOTINAMIDE-ADENINE-DINUCLEOTIDE × 1 ZN ZINC ION × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.54 Å
8CSS Human mitochondrial small subunit assembly intermediate (State D) Deposited 2022-05-13 Assembly 1 Protein–RNA Heteromer；Protein × 32 PDB declaration: 33-meric（33） Consistent with all polymers
Chain Q 1–87（87 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) SF4 IRON/SULFUR CLUSTER × 1 SAM S-ADENOSYLMETHIONINE × 1 K POTASSIUM ION × 7 MG MAGNESIUM ION × 32 NAD NICOTINAMIDE-ADENINE-DINUCLEOTIDE × 1 ZN ZINC ION × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.36 Å
8CST Human mitochondrial small subunit assembly intermediate (State E) Deposited 2022-05-13 Assembly 1 Protein–RNA Heteromer；Protein × 31 PDB declaration: 32-meric（32） Consistent with all polymers
Chain Q 1–87（87 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) SF4 IRON/SULFUR CLUSTER × 1 SAM S-ADENOSYLMETHIONINE × 1 K POTASSIUM ION × 4 MG MAGNESIUM ION × 30 NAD NICOTINAMIDE-ADENINE-DINUCLEOTIDE × 1 ZN ZINC ION × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.85 Å
8CSU Human mitochondrial small subunit assembly intermediate (State C*) Deposited 2022-05-13 Assembly 1 Protein–RNA Heteromer；Protein × 32 PDB declaration: 33-meric（33） Consistent with all polymers
Chain Q 1–87（87 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) SF4 IRON/SULFUR CLUSTER × 1 SAM S-ADENOSYLMETHIONINE × 1 K POTASSIUM ION × 5 MG MAGNESIUM ION × 26 NAD NICOTINAMIDE-ADENINE-DINUCLEOTIDE × 1 ZN ZINC ION × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.03 Å
8K2A Cryo-EM structure of the human 55S mitoribosome with Tigecycline Deposited 2023-07-12 Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 83-meric（83） Consistent with all polymers
Chain SW 1–87（87 aa）
Not recorded MG MAGNESIUM ION × 145 T1C TIGECYCLINE × 4 ZN ZINC ION × 7 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
8OIR 55S human mitochondrial ribosome with mtRF1 and P-site tRNA Deposited 2023-03-23 Assembly 1 Protein–RNA Heteromer；Protein × 88 PDB declaration: 94-meric（94） Consistent with all polymers
Chain AQ 1–87（87 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) K POTASSIUM ION × 51 MG MAGNESIUM ION × 354 VAL VALINE × 1 ZN ZINC ION × 3 FS2 FE-S-O HYBRID CLUSTER × 3 MET METHIONINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.10 Å
8OIS 28S human mitochondrial small ribosomal subunit with mtRF1 and P-site tRNA Deposited 2023-03-23 Assembly 1 Protein–RNA Heteromer；Protein × 33 PDB declaration: 36-meric（36） Consistent with all polymers
Chain AQ 1–87（87 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) K POTASSIUM ION × 17 MG MAGNESIUM ION × 126 MET METHIONINE × 1 ZN ZINC ION × 1 FS2 FE-S-O HYBRID CLUSTER × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.00 Å
8QRK mt-SSU assembly intermediate in GTPBP8 knock-out cells, state 1 Deposited 2023-10-09 Assembly 1 Protein–RNA Heteromer；Protein × 29 PDB declaration: 30-meric（30） Consistent with all polymers
Chain Q 2–87（86 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) MG MAGNESIUM ION × 49 NAD NICOTINAMIDE-ADENINE-DINUCLEOTIDE × 1 SPM SPERMINE × 1 SRY STREPTOMYCIN × 1 K POTASSIUM ION × 17 ZN ZINC ION × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 6.69 Å
8QRL mt-SSU assembly intermediate in GTPBP8 knock-out cells, state 2 Deposited 2023-10-09 Assembly 1 Protein–RNA Heteromer；Protein × 30 PDB declaration: 31-meric（31） Consistent with all polymers
Chain Q 2–87（86 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) NAD NICOTINAMIDE-ADENINE-DINUCLEOTIDE × 1 MG MAGNESIUM ION × 64 K POTASSIUM ION × 16 ZN ZINC ION × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 SAH S-ADENOSYL-L-HOMOCYSTEINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.34 Å
8QRM mt-SSU assembly intermediate in GTPBP8 knock-out cells, state 3 Deposited 2023-10-09 Assembly 1 Protein–RNA Heteromer；Protein × 31 PDB declaration: 32-meric（32） Consistent with all polymers
Chain Q 2–87（86 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) NAD NICOTINAMIDE-ADENINE-DINUCLEOTIDE × 1 SPM SPERMINE × 1 SRY STREPTOMYCIN × 1 MG MAGNESIUM ION × 59 K POTASSIUM ION × 17 ZN ZINC ION × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.05 Å
8QRN mt-SSU in GTPBP8 knock-out cells, state 4 Deposited 2023-10-09 Assembly 1 Protein–RNA Heteromer；Protein × 31 PDB declaration: 34-meric（34） Consistent with all polymers
Chain Q 2–87（86 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) NAD NICOTINAMIDE-ADENINE-DINUCLEOTIDE × 1 SPM SPERMINE × 1 SRY STREPTOMYCIN × 1 MG MAGNESIUM ION × 58 K POTASSIUM ION × 18 ZN ZINC ION × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 FME N-FORMYLMETHIONINE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.98 Å
8RRI Human mitochondrial ribosome in complex with antibiotic tigecycline Deposited 2024-01-22 Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 86-meric（86） Consistent with all polymers
Chain AQ 2–87（86 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) VAL VALINE × 1 MG MAGNESIUM ION × 94 FES FE2/S2 (INORGANIC) CLUSTER × 3 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 T1C TIGECYCLINE × 3 K POTASSIUM ION × 16 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.40 Å
8XT0 Cryo-EM structure of the human 55S mitoribosome with 5um Tigecycline Deposited 2024-01-10 Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 83-meric（83） Consistent with all polymers
Chain SW 1–87（87 aa）
Not recorded MG MAGNESIUM ION × 145 T1C TIGECYCLINE × 3 ZN ZINC ION × 7 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
8XT2 Cryo-EM structure of the human 55S mitoribosome with 10uM Tigecycline Deposited 2024-01-10 Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 83-meric（83） Consistent with all polymers
Chain SW 1–87（87 aa）
Not recorded MG MAGNESIUM ION × 145 T1C TIGECYCLINE × 3 ZN ZINC ION × 7 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
9G5B Assembly intermediate of human mitochondrial ribosome small subunit (State A) Deposited 2024-07-16 Assembly 1 Protein–RNA Heteromer；Protein × 31 PDB declaration: 32-meric（32） Consistent with all polymers
Chain Q 2–87（86 aa）
Not recorded MG MAGNESIUM ION × 12 ZN ZINC ION × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；20mM HEPES-HCl, 100mM KCl, 20mM MgCl2, 0.02% DDM, 1mM PMSF, 1x protease-inhibitor mix, 0.5mM GMP-PNP
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
9G5C Assembly intermediate of human mitochondrial ribosome small subunit (State B) Deposited 2024-07-16 Assembly 1 Protein–RNA Heteromer；Protein × 31 PDB declaration: 32-meric（32） Consistent with all polymers
Chain Q 2–87（86 aa）
Not recorded MG MAGNESIUM ION × 23 K POTASSIUM ION × 4 ZN ZINC ION × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 SAH S-ADENOSYL-L-HOMOCYSTEINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；20mM HEPES-HCl, 100mM KCl, 20mM MgCl2, 0.02% DDM, 1mM PMSF, 1x protease-inhibitor mix, 0.5mM GMP-PNP
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
9G5D Assembly intermediate of human mitochondrial ribosome small subunit (State C) Deposited 2024-07-16 Assembly 1 Protein–RNA Heteromer；Protein × 31 PDB declaration: 32-meric（32） Consistent with all polymers
Chain Q 1–87（87 aa）
Not recorded MG MAGNESIUM ION × 10 K POTASSIUM ION × 1 NAD NICOTINAMIDE-ADENINE-DINUCLEOTIDE × 1 ZN ZINC ION × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 SAH S-ADENOSYL-L-HOMOCYSTEINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；20mM HEPES-HCl, 100mM KCl, 20mM MgCl2, 0.02% DDM, 1mM PMSF, 1x protease-inhibitor mix, 0.5mM GMP-PNP
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
9G5E Translation-initiation state of human mitochondrial ribosome small subunit (State F) Deposited 2024-07-16 Assembly 1 Protein–RNA Heteromer；Protein × 31 PDB declaration: 32-meric（32） Consistent with all polymers
Chain Q 1–87（87 aa）
Not recorded MG MAGNESIUM ION × 45 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 K POTASSIUM ION × 1 ZN ZINC ION × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；20mM HEPES-HCl, 100mM KCl, 20mM MgCl2, 0.02% DDM, 1mM PMSF, 1x protease-inhibitor mix, 0.5mM GMP-PNP
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
9H51 Assembly intermediate of human mitochondrial ribosome small subunit in complex with PUS1, METTL15 and RBFA(IN conformation) (state M1) Deposited 2024-10-22 Assembly 1 Protein–RNA Heteromer；Protein × 33 PDB declaration: 34-meric（34） Consistent with all polymers
Chain Q 2–87（86 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) NAD NICOTINAMIDE-ADENINE-DINUCLEOTIDE × 1 SPM SPERMINE × 1 MG MAGNESIUM ION × 40 K POTASSIUM ION × 6 ZN ZINC ION × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 SAH S-ADENOSYL-L-HOMOCYSTEINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
9H54 Assembly intermediate of human mitochondrial ribosome small subunit in complex with NOA1 and partial RBFA (state N2) Deposited 2024-10-22 Assembly 1 Protein–RNA Heteromer；Protein × 30 PDB declaration: 31-meric（31） Consistent with all polymers
Chain Q 2–87（86 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) MG MAGNESIUM ION × 42 K POTASSIUM ION × 11 ZN ZINC ION × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
9H55 Assembly intermediate of human mitochondrial ribosome small subunit in complex with NOA1 and TFB1M (state N3) Deposited 2024-10-22 Assembly 1 Protein–RNA Heteromer；Protein × 31 PDB declaration: 32-meric（32） Consistent with all polymers
Chain Q 2–87（86 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) MG MAGNESIUM ION × 41 K POTASSIUM ION × 10 ZN ZINC ION × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
9HFM Translation-initiation state of human mitochondrial ribosome small subunit (State D) Deposited 2024-11-18 Assembly 1 Protein–RNA Heteromer；Protein × 31 PDB declaration: 32-meric（32） Consistent with all polymers
Chain Q 2–87（86 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) MG MAGNESIUM ION × 44 K POTASSIUM ION × 1 ZN ZINC ION × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；20mM HEPES-HCl, 100mM KCl, 20mM MgCl2, 0.02% DDM, 1mM PMSF, 1x protease-inhibitor mix, 0.5mM GMP-PNP
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
9HFN Translation-initiation state of human mitochondrial ribosome small subunit (State E) Deposited 2024-11-18 Assembly 1 Protein–RNA Heteromer；Protein × 32 PDB declaration: 33-meric（33） Consistent with all polymers
Chain Q 1–87（87 aa）
Not recorded MG MAGNESIUM ION × 45 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 K POTASSIUM ION × 1 ZN ZINC ION × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；20mM HEPES-HCl, 100mM KCl, 20mM MgCl2, 0.02% DDM, 1mM PMSF, 1x protease-inhibitor mix, 0.5mM GMP-PNP
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
9HFO Translation-initiation state of human mitochondrial ribosome small subunit (State G) Deposited 2024-11-18 Assembly 1 Protein–RNA Heteromer；Protein × 31 PDB declaration: 34-meric（34） Consistent with all polymers
Chain Q 1–87（87 aa）
Not recorded MG MAGNESIUM ION × 45 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 K POTASSIUM ION × 1 ZN ZINC ION × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；20mM HEPES-HCl, 100mM KCl, 20mM MgCl2, 0.02% DDM, 1mM PMSF, 1x protease-inhibitor mix, 0.5mM GMP-PNP
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
9OJM Human mitochondrial 28S PIC with tRNA and mtIF2 Deposited 2025-05-08 Assembly 1 Protein–RNA Heteromer；Protein × 31 PDB declaration: 34-meric（34） Consistent with all polymers
Chain Q 1–87（87 aa）
Not recorded MG MAGNESIUM ION × 72 K POTASSIUM ION × 20 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ZN ZINC ION × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.50 Å
9OLF Membrane-associated human mitoribosome in complex with TACO1 Deposited 2025-05-12 Assembly 1 Protein–RNA Heteromer；Protein × 93 PDB declaration: 100-meric（100） Consistent with all polymers
Chain AQ 2–87（86 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) ZN ZINC ION × 3 SPD SPERMIDINE × 7 MG MAGNESIUM ION × 205 PUT 1,4-DIAMINOBUTANE × 1 K POTASSIUM ION × 54 SPM SPERMINE × 2 NAD NICOTINAMIDE-ADENINE-DINUCLEOTIDE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 3 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 VAL VALINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.46 Å
9PG8 In situ structure of the human mitoribosome in the P-E state Deposited 2025-07-07 Assembly 1 Protein–RNA Heteromer；Protein × 86 PDB declaration: 93-meric（93） Consistent with all polymers
Chain AQ 2–87（86 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) ZN ZINC ION × 3 K POTASSIUM ION × 54 SPD SPERMIDINE × 8 PUT 1,4-DIAMINOBUTANE × 1 MG MAGNESIUM ION × 205 VAL VALINE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 3 NAD NICOTINAMIDE-ADENINE-DINUCLEOTIDE × 1 SPM SPERMINE × 3 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.06 Å
9PGF In situ structure of the human mitoribosome in the P state Deposited 2025-07-07 Assembly 1 Protein–RNA Heteromer；Protein × 86 PDB declaration: 91-meric（91） Consistent with all polymers
Chain AQ 2–87（86 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) ZN ZINC ION × 3 K POTASSIUM ION × 54 SPD SPERMIDINE × 8 PUT 1,4-DIAMINOBUTANE × 1 MG MAGNESIUM ION × 205 VAL VALINE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 3 NAD NICOTINAMIDE-ADENINE-DINUCLEOTIDE × 1 SPM SPERMINE × 3 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.93 Å
9PGI In situ structure of the human mitoribosome in the A-P-E state with TACO1 Deposited 2025-07-07 Assembly 1 Protein–RNA Heteromer；Protein × 87 PDB declaration: 94-meric（94） Consistent with all polymers
Chain AQ 2–87（86 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) ZN ZINC ION × 3 K POTASSIUM ION × 54 SPD SPERMIDINE × 7 PUT 1,4-DIAMINOBUTANE × 1 MG MAGNESIUM ION × 204 FES FE2/S2 (INORGANIC) CLUSTER × 3 NAD NICOTINAMIDE-ADENINE-DINUCLEOTIDE × 1 SPM SPERMINE × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 VAL VALINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.02 Å
9PGL In situ structure of the human mitoribosome in the A-P state with TACO1 Deposited 2025-07-08 Assembly 1 Protein–RNA Heteromer；Protein × 86 PDB declaration: 92-meric（92） Consistent with all polymers
Chain AQ 2–87（86 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) ZN ZINC ION × 3 K POTASSIUM ION × 54 SPD SPERMIDINE × 8 PUT 1,4-DIAMINOBUTANE × 1 MG MAGNESIUM ION × 204 FES FE2/S2 (INORGANIC) CLUSTER × 3 NAD NICOTINAMIDE-ADENINE-DINUCLEOTIDE × 1 SPM SPERMINE × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 VAL VALINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
9PGM In situ structure of the human mitoribosome in the P-E state from TACO1-knockout cells Deposited 2025-07-08 Assembly 1 Protein–RNA Heteromer；Protein × 85 PDB declaration: 91-meric（91） Consistent with all polymers
Chain AQ 2–87（86 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) ZN ZINC ION × 3 K POTASSIUM ION × 54 SPD SPERMIDINE × 7 PUT 1,4-DIAMINOBUTANE × 1 MG MAGNESIUM ION × 204 FES FE2/S2 (INORGANIC) CLUSTER × 3 NAD NICOTINAMIDE-ADENINE-DINUCLEOTIDE × 1 SPM SPERMINE × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 VAL VALINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.18 Å
9PRD In situ structure of the human mitoribosome in the P state from TACO1-knockout cells Deposited 2025-07-23 Assembly 1 Protein–RNA Heteromer；Protein × 84 PDB declaration: 89-meric（89） Consistent with all polymers
Chain AQ 2–87（86 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) ZN ZINC ION × 3 K POTASSIUM ION × 54 SPD SPERMIDINE × 7 PUT 1,4-DIAMINOBUTANE × 1 MG MAGNESIUM ION × 204 FES FE2/S2 (INORGANIC) CLUSTER × 3 NAD NICOTINAMIDE-ADENINE-DINUCLEOTIDE × 1 SPM SPERMINE × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 VAL VALINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.31 Å
9PRQ In situ structure of human mitoribosome in the A/T-P state from TACO1-knockout cells Deposited 2025-07-24 Assembly 1 Protein–RNA Heteromer；Protein × 85 PDB declaration: 91-meric（91） Consistent with all polymers
Chain AQ 2–87（86 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) ZN ZINC ION × 3 K POTASSIUM ION × 54 SPD SPERMIDINE × 7 PUT 1,4-DIAMINOBUTANE × 1 MG MAGNESIUM ION × 204 FES FE2/S2 (INORGANIC) CLUSTER × 3 NAD NICOTINAMIDE-ADENINE-DINUCLEOTIDE × 1 SPM SPERMINE × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 VAL VALINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.34 Å
9PRX In situ structure of the human mitoribosome in the A-P state from TACO1-knockout cells Deposited 2025-07-24 Assembly 1 Protein–RNA Heteromer；Protein × 84 PDB declaration: 90-meric（90） Consistent with all polymers
Chain AQ 2–87（86 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) VAL VALINE × 1 ZN ZINC ION × 3 K POTASSIUM ION × 54 SPD SPERMIDINE × 7 PUT 1,4-DIAMINOBUTANE × 1 MG MAGNESIUM ION × 204 FES FE2/S2 (INORGANIC) CLUSTER × 3 NAD NICOTINAMIDE-ADENINE-DINUCLEOTIDE × 1 SPM SPERMINE × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.23 Å
9PS0 In situ structure of the human mitoribosome in the A/P-P/E state from TACO1-knockout cells Deposited 2025-07-24 Assembly 1 Protein–RNA Heteromer；Protein × 85 PDB declaration: 91-meric（91） Consistent with all polymers
Chain AQ 2–87（86 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) ZN ZINC ION × 3 K POTASSIUM ION × 52 SPD SPERMIDINE × 7 PUT 1,4-DIAMINOBUTANE × 1 MG MAGNESIUM ION × 204 NAD NICOTINAMIDE-ADENINE-DINUCLEOTIDE × 1 SPM SPERMINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 3 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 VAL VALINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.29 Å
9PS7 In situ structure of the human mitoribosome in the A/T-P-E state from TACO1-knockout cells Deposited 2025-07-25 Assembly 1 Protein–RNA Heteromer；Protein × 86 PDB declaration: 93-meric（93） Consistent with all polymers
Chain AQ 2–87（86 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) ZN ZINC ION × 3 K POTASSIUM ION × 54 SPD SPERMIDINE × 7 PUT 1,4-DIAMINOBUTANE × 1 MG MAGNESIUM ION × 204 FES FE2/S2 (INORGANIC) CLUSTER × 3 NAD NICOTINAMIDE-ADENINE-DINUCLEOTIDE × 1 SPM SPERMINE × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 VAL VALINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.08 Å
9PSI In situ structure of the human mitoribosome in the A-P-E state from TACO1-knockout cells Deposited 2025-07-25 Assembly 1 Protein–RNA Heteromer；Protein × 85 PDB declaration: 92-meric（92） Consistent with all polymers
Chain AQ 2–87（86 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) ZN ZINC ION × 3 K POTASSIUM ION × 54 SPD SPERMIDINE × 7 PUT 1,4-DIAMINOBUTANE × 1 MG MAGNESIUM ION × 204 FES FE2/S2 (INORGANIC) CLUSTER × 3 NAD NICOTINAMIDE-ADENINE-DINUCLEOTIDE × 1 SPM SPERMINE × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 VAL VALINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.12 Å
9PSM In situ structure of the human mitoribosome in the A/P-P/E state Deposited 2025-07-25 Assembly 1 Protein–RNA Heteromer；Protein × 87 PDB declaration: 93-meric（93） Consistent with all polymers
Chain AQ 2–87（86 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) ZN ZINC ION × 3 K POTASSIUM ION × 54 SPD SPERMIDINE × 8 PUT 1,4-DIAMINOBUTANE × 1 MG MAGNESIUM ION × 204 FES FE2/S2 (INORGANIC) CLUSTER × 3 NAD NICOTINAMIDE-ADENINE-DINUCLEOTIDE × 1 SPM SPERMINE × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 VAL VALINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.98 Å

This page presents structural evidence recorded in PDB/mmCIF.“No recorded non-water small molecule” does not prove that the experimental sample was ligand-free; experimental conditions belong to the PDB experiment, while ligands and copy counts are shown for each biological assembly.