Current Protein Identity：Q81HW2
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Difference tags compare only the current result set; every original PDB and assembly record remains separate.
Related-Structure Differences
Each row represents one biological assembly in one PDB entry; multiple monomers of the same protein are listed separately.
| PDB Entry | Assembly / Oligomeric State | Construct | Mutations and Modifications | Ligands, Ions and Non-polymers | Experimental Method | Experimental Conditions | Structure Quality |
|---|---|---|---|---|---|---|---|
| 2Q67 Crystal Structure of Nak channel D66A mutant Deposited 2007-06-04 | Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain A
1–110（110 aa）
Chain B
1–110（110 aa）
|
Mutation：D66A Mutation：D66A | NA SODIUM ION × 2 CA CALCIUM ION × 4 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8；298 K；200 mM CaCl2, 100 mM Tris-HCl, 37-42% PEG400, 4% t-Butanol, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K
|
Resolution 2.30 Å R-free 0.266 |
| 2Q68 Crystal Structure of Nak channel D66A, S70E double mutants Deposited 2007-06-04 | Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain A
1–110（110 aa）
Chain B
1–110（110 aa）
|
Mutation：D66A, S70E Mutation：D66A, S70E | CA CALCIUM ION × 4 NA SODIUM ION × 6 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8；298 K；200 mM CaCl2, 100 mM Tris-HCl, 37-42% PEG400, 4% t-Butanol, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K
|
Resolution 2.50 Å R-free 0.262 |
| 2Q69 Crystal Structure of Nak channel D66N mutant Deposited 2007-06-04 | Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain A
1–110（110 aa）
Chain B
1–110（110 aa）
|
Mutation：D66N Mutation：D66N | NA SODIUM ION × 4 CA CALCIUM ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8；298 K；200 mM CaCl2, 100 mM Tris-HCl, 37-42% PEG400, 4% t-Butanol, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K
|
Resolution 2.40 Å R-free 0.267 |
| 2Q6A Crystal Structure of Nak channel D66E mutant Deposited 2007-06-04 | Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain A
1–110（110 aa）
Chain B
1–110（110 aa）
|
Mutation：D66E Mutation：D66E | CA CALCIUM ION × 4 NA SODIUM ION × 6 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8；298 K；200 mM CaCl2, 100 mM Tris-HCl, 37-42% PEG400, 4% t-Butanol, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K
|
Resolution 2.60 Å R-free 0.280 |
| 3E83 Crystal Structure of the the open NaK channel pore Deposited 2008-08-19 | Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain A
19–110（92 aa）
Fragment：transmembrane domain, residues 19-110
|
Not recorded | CS CESIUM ION × 4 NA SODIUM ION × 8 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.5；293 K；100mM Hepes, 55-70% (4S)-2-Methyl-2,4-pentanediol (MPD), 500mM NaCl, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K
|
Resolution 1.80 Å R-free 0.236 |
| 3E83 Crystal Structure of the the open NaK channel pore Deposited 2008-08-19 | Assembly 2 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain B
19–110（92 aa）
Fragment：transmembrane domain, residues 19-110
|
Not recorded | CS CESIUM ION × 4 NA SODIUM ION × 4 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.5；293 K；100mM Hepes, 55-70% (4S)-2-Methyl-2,4-pentanediol (MPD), 500mM NaCl, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K
|
Resolution 1.80 Å R-free 0.236 |
| 3E86 High resolution Crystal Structure of the open NaK channel pore Deposited 2008-08-19 | Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain A
19–110（92 aa）
Fragment：transmembrane domain, RESIDUES 19-110
|
Not recorded | MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 4 CS CESIUM ION × 4 CA CALCIUM ION × 4 NA SODIUM ION × 8 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 9.5；293 K；100mM Glycine buffer, 55-70% (4S)-2-Methyl-2,4-pentanediol (MPD), 1mM CaCl2, pH 9.5, vapor diffusion, sitting drop, temperature 293K, VAPOR DIFFUSION, SITTING DROP
|
Resolution 1.60 Å R-free 0.242 |
| 3E86 High resolution Crystal Structure of the open NaK channel pore Deposited 2008-08-19 | Assembly 2 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain B
19–110（92 aa）
Fragment：transmembrane domain, RESIDUES 19-110
|
Not recorded | MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 4 CS CESIUM ION × 4 NA SODIUM ION × 8 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 9.5；293 K；100mM Glycine buffer, 55-70% (4S)-2-Methyl-2,4-pentanediol (MPD), 1mM CaCl2, pH 9.5, vapor diffusion, sitting drop, temperature 293K, VAPOR DIFFUSION, SITTING DROP
|
Resolution 1.60 Å R-free 0.242 |
| 3E89 Crystal Structure of the the open NaK channel-low Na+ complex Deposited 2008-08-19 | Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain A
19–110（92 aa）
Fragment：transmembrane domain, residues 19-110
|
Not recorded | MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 8 CS CESIUM ION × 4 NA SODIUM ION × 8 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.5；293 K；100mM Hepes buffer, 55-70% (4S)-2-Methyl-2,4-pentanediol (MPD), pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K
|
Resolution 1.80 Å R-free 0.248 |
| 3E89 Crystal Structure of the the open NaK channel-low Na+ complex Deposited 2008-08-19 | Assembly 2 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain B
19–110（92 aa）
Fragment：transmembrane domain, residues 19-110
|
Not recorded | MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 4 CS CESIUM ION × 4 NA SODIUM ION × 8 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.5；293 K；100mM Hepes buffer, 55-70% (4S)-2-Methyl-2,4-pentanediol (MPD), pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K
|
Resolution 1.80 Å R-free 0.248 |
| 3E8B Crystal Structure of the the open NaK channel- Rb+ complex Deposited 2008-08-19 | Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain A
19–110（92 aa）
Fragment：transmembrane domain, residues 19-110
|
Not recorded | MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 8 RB RUBIDIUM ION × 24 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.5；293 K；100mM Hepes buffer, 55-70% (4S)-2-Methyl-2,4-pentanediol (MPD), pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K
|
Resolution 1.70 Å R-free 0.229 |
| 3E8B Crystal Structure of the the open NaK channel- Rb+ complex Deposited 2008-08-19 | Assembly 2 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain B
19–110（92 aa）
Fragment：transmembrane domain, residues 19-110
|
Not recorded | MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 8 RB RUBIDIUM ION × 20 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.5；293 K；100mM Hepes buffer, 55-70% (4S)-2-Methyl-2,4-pentanediol (MPD), pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K
|
Resolution 1.70 Å R-free 0.229 |
| 3E8F Crystal Structure of the the open NaK channel- K+/Ba2+ Deposited 2008-08-19 | Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain A
19–110（92 aa）
Fragment：transmembrane domain, residues 19-110
|
Not recorded | MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 4 BA BARIUM ION × 8 K POTASSIUM ION × 12 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.5；293 K；100mM Hepes Buffer, 55-70% (4S)-2-Methyl-2,4-pentanediol (MPD),
Soaked in stabilization solution containing 67mM KCl and 33mM BaCl2, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K
|
Resolution 2.00 Å R-free 0.248 |
| 3E8F Crystal Structure of the the open NaK channel- K+/Ba2+ Deposited 2008-08-19 | Assembly 2 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain B
19–110（92 aa）
Fragment：transmembrane domain, residues 19-110
|
Not recorded | MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 4 BA BARIUM ION × 8 K POTASSIUM ION × 12 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.5；293 K；100mM Hepes Buffer, 55-70% (4S)-2-Methyl-2,4-pentanediol (MPD),
Soaked in stabilization solution containing 67mM KCl and 33mM BaCl2, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K
|
Resolution 2.00 Å R-free 0.248 |
| 3E8G Crystal Structure of the the open NaK channel-Na+/Ca2+ complex Deposited 2008-08-19 | Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain A
19–110（92 aa）
Fragment：transmembrane domain, residues 19-110
|
Not recorded | MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 8 CS CESIUM ION × 4 CA CALCIUM ION × 4 NA SODIUM ION × 8 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.5；293 K；100mM Hepes, 55-70% (4S)-2-Methyl-2,4-pentanediol (MPD), 10mM CaCl2, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K
|
Resolution 2.00 Å R-free 0.235 |
| 3E8G Crystal Structure of the the open NaK channel-Na+/Ca2+ complex Deposited 2008-08-19 | Assembly 2 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain B
19–110（92 aa）
Fragment：transmembrane domain, residues 19-110
|
Not recorded | MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 4 CS CESIUM ION × 4 CA CALCIUM ION × 4 NA SODIUM ION × 8 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.5；293 K；100mM Hepes, 55-70% (4S)-2-Methyl-2,4-pentanediol (MPD), 10mM CaCl2, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K
|
Resolution 2.00 Å R-free 0.235 |
| 3E8H Crystal Structure of the the open NaK channel-K+ complex Deposited 2008-08-19 | Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain A
19–110（92 aa）
Fragment：transmembrane domain, residues 19-110
|
Not recorded | MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 8 K POTASSIUM ION × 24 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.5；293 K；100mM GHepes buffer, 55-70% (4S)-2-Methyl-2,4-pentanediol (MPD), pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K
|
Resolution 1.80 Å R-free 0.234 |
| 3E8H Crystal Structure of the the open NaK channel-K+ complex Deposited 2008-08-19 | Assembly 2 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain B
19–110（92 aa）
Fragment：transmembrane domain, residues 19-110
|
Not recorded | MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 8 K POTASSIUM ION × 16 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.5；293 K；100mM GHepes buffer, 55-70% (4S)-2-Methyl-2,4-pentanediol (MPD), pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K
|
Resolution 1.80 Å R-free 0.234 |
| 3K03 Crystal Structure of CNG mimicking NaK mutant, NaK-DTPP, K+ complex Deposited 2009-09-24 | Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain A
20–110（91 aa）
|
Not recorded | MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 12 K POTASSIUM ION × 20 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；293 K；MPD, KCl, pH 6.5-8.5, vapor diffusion, temperature 293K
|
Resolution 1.62 Å R-free 0.222 |
| 3K03 Crystal Structure of CNG mimicking NaK mutant, NaK-DTPP, K+ complex Deposited 2009-09-24 | Assembly 2 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain B
20–110（91 aa）
|
Not recorded | MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 8 K POTASSIUM ION × 16 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；293 K；MPD, KCl, pH 6.5-8.5, vapor diffusion, temperature 293K
|
Resolution 1.62 Å R-free 0.222 |
| 3K04 Crystal Structure of CNG mimicking NaK mutant, NaK-DTPP, Na+ complex Deposited 2009-09-24 | Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain A
20–110（91 aa）
|
Not recorded | MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 16 NA SODIUM ION × 24 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；293 K；MPD, NaCl, pH 6.5-8.5, vapor diffusion, temperature 293K
|
Resolution 1.58 Å R-free 0.235 |
| 3K04 Crystal Structure of CNG mimicking NaK mutant, NaK-DTPP, Na+ complex Deposited 2009-09-24 | Assembly 2 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain B
20–110（91 aa）
|
Not recorded | MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 8 NA SODIUM ION × 8 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；293 K；MPD, NaCl, pH 6.5-8.5, vapor diffusion, temperature 293K
|
Resolution 1.58 Å R-free 0.235 |
| 3K06 Crystal Structure of CNG mimicking NaK mutant, NaK-NTPP, K+ complex Deposited 2009-09-24 | Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain A
20–110（91 aa）
|
Not recorded | MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 20 K POTASSIUM ION × 20 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；293 K；MPD, KCl, pH 6.5-8.5, vapor diffusion, temperature 293K
|
Resolution 1.58 Å R-free 0.214 |
| 3K06 Crystal Structure of CNG mimicking NaK mutant, NaK-NTPP, K+ complex Deposited 2009-09-24 | Assembly 2 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain B
20–110（91 aa）
|
Not recorded | MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 8 K POTASSIUM ION × 16 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；293 K；MPD, KCl, pH 6.5-8.5, vapor diffusion, temperature 293K
|
Resolution 1.58 Å R-free 0.214 |
| 3K08 Crystal Structure of CNG mimicking NaK mutant, NaK-NTPP, Na+ complex Deposited 2009-09-24 | Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain A
20–110（91 aa）
|
Not recorded | MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 16 NA SODIUM ION × 24 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；293 K；MPD, NaCl, pH 6.5-8.5, vapor diffusion, temperature 293K
|
Resolution 1.62 Å R-free 0.227 |
| 3K08 Crystal Structure of CNG mimicking NaK mutant, NaK-NTPP, Na+ complex Deposited 2009-09-24 | Assembly 2 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain B
20–110（91 aa）
|
Not recorded | MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 8 NA SODIUM ION × 16 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；293 K；MPD, NaCl, pH 6.5-8.5, vapor diffusion, temperature 293K
|
Resolution 1.62 Å R-free 0.227 |
| 3K0D Crystal Structure of CNG mimicking NaK mutant, NaK-ETPP, K+ complex Deposited 2009-09-24 | Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain A
20–110（91 aa）
|
Not recorded | MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 12 K POTASSIUM ION × 20 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；293 K；MPD, KCl, pH 6.5-8.5, vapor diffusion, temperature 293K
|
Resolution 1.95 Å R-free 0.239 |
| 3K0D Crystal Structure of CNG mimicking NaK mutant, NaK-ETPP, K+ complex Deposited 2009-09-24 | Assembly 2 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain B
20–110（91 aa）
|
Not recorded | MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 4 K POTASSIUM ION × 16 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；293 K；MPD, KCl, pH 6.5-8.5, vapor diffusion, temperature 293K
|
Resolution 1.95 Å R-free 0.239 |
| 3K0G Crystal Structure of CNG mimicking NaK mutant, NaK-ETPP, Na+ complex Deposited 2009-09-24 | Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain A
20–110（91 aa）
|
Not recorded | MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 12 NA SODIUM ION × 16 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；293 K；MPD, NaCl, pH 6.5-8.5, vapor diffusion, temperature 293K
|
Resolution 1.95 Å R-free 0.228 |
| 3K0G Crystal Structure of CNG mimicking NaK mutant, NaK-ETPP, Na+ complex Deposited 2009-09-24 | Assembly 2 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain B
20–110（91 aa）
|
Not recorded | MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 8 NA SODIUM ION × 12 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；293 K；MPD, NaCl, pH 6.5-8.5, vapor diffusion, temperature 293K
|
Resolution 1.95 Å R-free 0.228 |
| 3T1C Crystal Structure of NaK Channel D66Y Mutant Deposited 2011-07-21 | Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain A
20–110（91 aa）
|
Mutation：D66Y | K POTASSIUM ION × 20 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7；293 K；100mM Tris, 70% MPD, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K
|
Resolution 1.80 Å R-free 0.233 |
| 3T1C Crystal Structure of NaK Channel D66Y Mutant Deposited 2011-07-21 | Assembly 2 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain B
20–110（91 aa）
|
Mutation：D66Y | K POTASSIUM ION × 20 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7；293 K；100mM Tris, 70% MPD, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K
|
Resolution 1.80 Å R-free 0.233 |
| 3T2M Crystal Structure of NaK Channel N68D Mutant Deposited 2011-07-22 | Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain A
20–110（91 aa）
|
Mutation：N68D | K POTASSIUM ION × 12 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；70% MPD, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K
|
Resolution 1.95 Å R-free 0.223 |
| 3T2M Crystal Structure of NaK Channel N68D Mutant Deposited 2011-07-22 | Assembly 2 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain B
20–110（91 aa）
|
Mutation：N68D | K POTASSIUM ION × 16 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；70% MPD, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K
|
Resolution 1.95 Å R-free 0.223 |
| 3T4D Crystal Structure of NaK2K Channel Y55F Mutant Deposited 2011-07-25 | Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain A
20–110（91 aa）
|
Mutation：Y55F, D66Y, N68D | K POTASSIUM ION × 16 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 9.5；293 K；65% MPD, 100mM Glycine, pH 9.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K
|
Resolution 1.70 Å R-free 0.225 |
| 3T4D Crystal Structure of NaK2K Channel Y55F Mutant Deposited 2011-07-25 | Assembly 2 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain B
20–110（91 aa）
|
Mutation：Y55F, D66Y, N68D | K POTASSIUM ION × 16 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 9.5；293 K；65% MPD, 100mM Glycine, pH 9.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K
|
Resolution 1.70 Å R-free 0.225 |
| 3T4Z Crystal Structure of NaK2K Channel Y55W Mutant Deposited 2011-07-26 | Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain A
20–110（91 aa）
|
Mutation：Y55W, D66Y, N68D | K POTASSIUM ION × 16 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 9；293 K；65% MPD, 100mM Glycine, pH 9.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K
|
Resolution 1.90 Å R-free 0.241 |
| 3T4Z Crystal Structure of NaK2K Channel Y55W Mutant Deposited 2011-07-26 | Assembly 2 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain B
20–110（91 aa）
|
Mutation：Y55W, D66Y, N68D | K POTASSIUM ION × 16 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 9；293 K；65% MPD, 100mM Glycine, pH 9.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K
|
Resolution 1.90 Å R-free 0.241 |
| 3TCU Crystal Structure of NaK2K Channel D68E Mutant Deposited 2011-08-09 | Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain A
20–110（91 aa）
|
Mutation：D66Y, N68E | K POTASSIUM ION × 16 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 9.5；293 K；60% MPD, 100mM Glycine, pH 9.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K
|
Resolution 1.75 Å R-free 0.225 |
| 3TCU Crystal Structure of NaK2K Channel D68E Mutant Deposited 2011-08-09 | Assembly 2 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain B
20–110（91 aa）
|
Mutation：D66Y, N68E | K POTASSIUM ION × 16 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 9.5；293 K；60% MPD, 100mM Glycine, pH 9.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K
|
Resolution 1.75 Å R-free 0.225 |
| 3TET Crystal Structure of NaK2K Channel Y66F Mutant Deposited 2011-08-15 | Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain A
20–110（91 aa）
|
Mutation：D66F, N68D | K POTASSIUM ION × 16 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 6；293 K；77.5% MPD, 100mM MES, 100mM KCl, pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K
|
Resolution 1.90 Å R-free 0.239 |
| 3TET Crystal Structure of NaK2K Channel Y66F Mutant Deposited 2011-08-15 | Assembly 2 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain B
20–110（91 aa）
|
Mutation：D66F, N68D | K POTASSIUM ION × 16 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 6；293 K；77.5% MPD, 100mM MES, 100mM KCl, pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K
|
Resolution 1.90 Å R-free 0.239 |
| 4PDL Structure of K+ selective NaK mutant in caesium Deposited 2014-04-19 | Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain A
20–110（91 aa）
Fragment：UNP residues 20-110
|
Mutation：D66Y,N68D | CS CESIUM ION × 12 NA SODIUM ION × 4 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 8 HEX HEXANE × 4 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 6.5；293 K；65% MPD, 100mM CsCl, 100mM MES, 4mM DM
|
Resolution 1.70 Å R-free 0.236 |
| 4PDL Structure of K+ selective NaK mutant in caesium Deposited 2014-04-19 | Assembly 2 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain B
20–110（91 aa）
Fragment：UNP residues 20-110
|
Mutation：D66Y,N68D | CS CESIUM ION × 8 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 4 HEX HEXANE × 4 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 6.5；293 K；65% MPD, 100mM CsCl, 100mM MES, 4mM DM
|
Resolution 1.70 Å R-free 0.236 |
| 4PDM Crystal Structure of K+ selective NaK mutant in rubidium Deposited 2014-04-19 | Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain A
20–110（91 aa）
Fragment：UNP residues 20-110
|
Mutation：D66Y,N68D | RB RUBIDIUM ION × 12 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 4 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 6；293 K；65%MPD, 100mM KCl, 100mM MES, 4mM n-Decyl-beta-D-Maltoside
|
Resolution 1.58 Å R-free 0.234 |
| 4PDM Crystal Structure of K+ selective NaK mutant in rubidium Deposited 2014-04-19 | Assembly 2 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain B
20–110（91 aa）
Fragment：UNP residues 20-110
|
Mutation：D66Y,N68D | RB RUBIDIUM ION × 12 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 8 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 6；293 K；65%MPD, 100mM KCl, 100mM MES, 4mM n-Decyl-beta-D-Maltoside
|
Resolution 1.58 Å R-free 0.234 |
| 4PDR Crystal Structure of a K+ selective NaK mutant in Barium and Sodium Deposited 2014-04-21 | Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain A
20–110（91 aa）
Fragment：UNP residues 20-110
|
Mutation：D66Y,N68D | BA BARIUM ION × 12 NA SODIUM ION × 4 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 4 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 6；293 K；65%MPD, 100mM KCl, 100mM MES, 4mM n-Decyl-beta-D-Maltoside
|
Resolution 1.85 Å R-free 0.227 |
| 4PDR Crystal Structure of a K+ selective NaK mutant in Barium and Sodium Deposited 2014-04-21 | Assembly 2 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain B
20–110（91 aa）
Fragment：UNP residues 20-110
|
Mutation：D66Y,N68D | BA BARIUM ION × 12 NA SODIUM ION × 4 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 12 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 6；293 K；65%MPD, 100mM KCl, 100mM MES, 4mM n-Decyl-beta-D-Maltoside
|
Resolution 1.85 Å R-free 0.227 |
| 4PDV Structure of K+ selective NaK mutant in barium and potassium Deposited 2014-04-22 | Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain A
20–110（91 aa）
Fragment：UNP residues 20-110
|
Mutation：D66Y, N68D | K POTASSIUM ION × 12 BA BARIUM ION × 8 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 8 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 6；293 K；65%MPD, 100mM KCl, 100mM MES, 4mM n-Decyl-beta-D-Maltoside
|
Resolution 1.82 Å R-free 0.232 |
| 4PDV Structure of K+ selective NaK mutant in barium and potassium Deposited 2014-04-22 | Assembly 2 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain B
20–110（91 aa）
Fragment：UNP residues 20-110
|
Mutation：D66Y, N68D | K POTASSIUM ION × 12 BA BARIUM ION × 4 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 8 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 6；293 K；65%MPD, 100mM KCl, 100mM MES, 4mM n-Decyl-beta-D-Maltoside
|
Resolution 1.82 Å R-free 0.232 |
| 4R50 Crystal Structure of CNG mimicking NaK-ETPP mutant cocrystallized with Li+ Deposited 2014-08-20 | Assembly 1 Protein homooligomer Homooligomer；Protein × 8 PDB declaration: octameric（8） Consistent with protein count |
Chain A
20–110（91 aa）
Fragment：residues 20-110
Chain B
20–110（91 aa）
Fragment：residues 20-110
|
Mutation：D66E, G67T, N68P, F69P Mutation：D66E, G67T, N68P, F69P | MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 4 GLY GLYCINE × 24 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；40-70% MPD, 20-100mM Glycine, pH 6.5 - 7.5, VAPOR DIFFUSION, HANGING DROP
|
Resolution 2.85 Å R-free 0.183 |
| 4R50 Crystal Structure of CNG mimicking NaK-ETPP mutant cocrystallized with Li+ Deposited 2014-08-20 | Assembly 2 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain A
20–110（91 aa）
Fragment：residues 20-110
|
Mutation：D66E, G67T, N68P, F69P | MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 4 GLY GLYCINE × 12 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；40-70% MPD, 20-100mM Glycine, pH 6.5 - 7.5, VAPOR DIFFUSION, HANGING DROP
|
Resolution 2.85 Å R-free 0.183 |
| 4R50 Crystal Structure of CNG mimicking NaK-ETPP mutant cocrystallized with Li+ Deposited 2014-08-20 | Assembly 3 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain B
20–110（91 aa）
Fragment：residues 20-110
|
Mutation：D66E, G67T, N68P, F69P | GLY GLYCINE × 12 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；40-70% MPD, 20-100mM Glycine, pH 6.5 - 7.5, VAPOR DIFFUSION, HANGING DROP
|
Resolution 2.85 Å R-free 0.183 |
| 4R6Z Crystal Structure of CNG mimicking NaK mutant, NaK-ETPP, Cs+ complex Deposited 2014-08-26 | Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain A
20–110（91 aa）
Fragment：residues 20-110
|
Mutation：D66E, G67T, N68P, F69P | CS CESIUM ION × 8 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 8 GLY GLYCINE × 20 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；CNG-ETPP(K+) crystals grown in 100mM MES pH 6.5, 60-66% (w/v) MPD, 100mM Glycine. Soaking overnight in 70% MPD, 10mM
DM, 100mM Hepes pH 7.5 and 100mM CsCl, VAPOR DIFFUSION, HANGING DROP
|
Resolution 2.30 Å R-free 0.182 |
| 4R6Z Crystal Structure of CNG mimicking NaK mutant, NaK-ETPP, Cs+ complex Deposited 2014-08-26 | Assembly 2 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain B
20–110（91 aa）
Fragment：residues 20-110
|
Mutation：D66E, G67T, N68P, F69P | CS CESIUM ION × 12 GLY GLYCINE × 28 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；CNG-ETPP(K+) crystals grown in 100mM MES pH 6.5, 60-66% (w/v) MPD, 100mM Glycine. Soaking overnight in 70% MPD, 10mM
DM, 100mM Hepes pH 7.5 and 100mM CsCl, VAPOR DIFFUSION, HANGING DROP
|
Resolution 2.30 Å R-free 0.182 |
| 4R7C Crystal Structure of CNG mimicking NaK-ETPP mutant cocrystallized with DiMethylammonium Deposited 2014-08-27 | Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain A
20–110（91 aa）
Fragment：residues 20-110
Chain B
20–110（91 aa）
Fragment：residues 20-110
|
Mutation：D66E, G67T, N68P, F69P Mutation：D66E, G67T, N68P, F69P | GLY GLYCINE × 32 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 14 DMN DIMETHYLAMINE × 32 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 6.5；CNG-ETPP(DiMA+) cocrystals grown in 40-44% MPD, 100mM MES pH6.5 and 20-25 mM Glycine, VAPOR DIFFUSION, HANGING DROP
|
Resolution 2.30 Å R-free 0.248 |
| 4R7C Crystal Structure of CNG mimicking NaK-ETPP mutant cocrystallized with DiMethylammonium Deposited 2014-08-27 | Assembly 2 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain C
20–110（91 aa）
Fragment：residues 20-110
Chain D
20–110（91 aa）
Fragment：residues 20-110
|
Mutation：D66E, G67T, N68P, F69P Mutation：D66E, G67T, N68P, F69P | GLY GLYCINE × 26 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 4 DMN DIMETHYLAMINE × 18 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 6.5；CNG-ETPP(DiMA+) cocrystals grown in 40-44% MPD, 100mM MES pH6.5 and 20-25 mM Glycine, VAPOR DIFFUSION, HANGING DROP
|
Resolution 2.30 Å R-free 0.248 |
| 4R8C Crystal Structure of CNG mimicking NaK-ETPP mutant in complex with Rb+ Deposited 2014-09-01 | Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain A
20–110（91 aa）
Fragment：residues 20-110
|
Mutation：D66E, G67T, N68P, F69P | RB RUBIDIUM ION × 12 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 4 GLY GLYCINE × 20 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；CNG-ETPP(K+) crystals grown in 100mM MES pH 6.5, 60-66% (w/v) MPD, 100mM Glycine, Soaking O.N. in 70% MPD, 10mM DM,
100mM Hepes pH 7.5 and 100mM RbCl, VAPOR DIFFUSION, HANGING DROP
|
Resolution 2.50 Å R-free 0.257 |
| 4R8C Crystal Structure of CNG mimicking NaK-ETPP mutant in complex with Rb+ Deposited 2014-09-01 | Assembly 2 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain B
20–110（91 aa）
Fragment：residues 20-110
|
Mutation：D66E, G67T, N68P, F69P | RB RUBIDIUM ION × 12 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 4 GLY GLYCINE × 20 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；CNG-ETPP(K+) crystals grown in 100mM MES pH 6.5, 60-66% (w/v) MPD, 100mM Glycine, Soaking O.N. in 70% MPD, 10mM DM,
100mM Hepes pH 7.5 and 100mM RbCl, VAPOR DIFFUSION, HANGING DROP
|
Resolution 2.50 Å R-free 0.257 |
| 4RAI Crystal Structure of CNG mimicking NaK-ETPP mutant in complex with Na+ Deposited 2014-09-10 | Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain A
20–110（91 aa）
Fragment：residues 20-110
|
Mutation：D66E, G67T, N68P, F69P | NA SODIUM ION × 16 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 8 GLY GLYCINE × 12 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；CNG-ETPP(K+) crystals grown in 100mM MES pH 6.5, 60-66% (w/v) MPD, 100mM Glycine, Soaking O.N. in 70% MPD, 10mM DM,
100mM Hepes pH 7.5 and 100mM NaCl, VAPOR DIFFUSION, HANGING DROP
|
Resolution 2.31 Å R-free 0.215 |
| 4RAI Crystal Structure of CNG mimicking NaK-ETPP mutant in complex with Na+ Deposited 2014-09-10 | Assembly 2 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain B
20–110（91 aa）
Fragment：residues 20-110
|
Mutation：D66E, G67T, N68P, F69P | NA SODIUM ION × 12 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 12 GLY GLYCINE × 28 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；CNG-ETPP(K+) crystals grown in 100mM MES pH 6.5, 60-66% (w/v) MPD, 100mM Glycine, Soaking O.N. in 70% MPD, 10mM DM,
100mM Hepes pH 7.5 and 100mM NaCl, VAPOR DIFFUSION, HANGING DROP
|
Resolution 2.31 Å R-free 0.215 |
| 4RO2 Crystal Structure of CNG mimicking NaK-ETPP mutant cocrystallized with Methylammonium Deposited 2014-10-27 | Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain A
20–110（91 aa）
Fragment：residues 20-110
Chain B
20–110（91 aa）
Fragment：residues 20-110
|
Mutation：D66E, G67T, N68P, F69P Mutation：D66E, G67T, N68P, F69P | 3P8 methylammonium ion × 2 GLY GLYCINE × 34 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 4 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；100mM MES pH 6.5, 25mM Glycine, 40-44% MPD, 100mM MACl, VAPOR DIFFUSION, HANGING DROP
|
Resolution 2.70 Å R-free 0.283 |
| 4RO2 Crystal Structure of CNG mimicking NaK-ETPP mutant cocrystallized with Methylammonium Deposited 2014-10-27 | Assembly 2 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain C
20–110（91 aa）
Fragment：residues 20-110
Chain D
20–110（91 aa）
Fragment：residues 20-110
|
Mutation：D66E, G67T, N68P, F69P Mutation：D66E, G67T, N68P, F69P | 3P8 methylammonium ion × 2 GLY GLYCINE × 22 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 4 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；100mM MES pH 6.5, 25mM Glycine, 40-44% MPD, 100mM MACl, VAPOR DIFFUSION, HANGING DROP
|
Resolution 2.70 Å R-free 0.283 |
| 4ZBM Crystal structure of Drosophila cyclic nucleotide gated channel pore mimicking NaK mutant Deposited 2015-04-14 | Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain A
20–110（91 aa）
Fragment：UNP residues 22-110
|
Not recorded | K POTASSIUM ION × 12 BA BARIUM ION × 4 | X-RAY DIFFRACTION |
X-ray crystallization conditions
EVAPORATION；293 K；65%MPD, 100mM KCl, 100mM MES, 4mM n-Decyl-beta-D-Maltoside
|
Resolution 1.90 Å R-free 0.236 |
| 4ZBM Crystal structure of Drosophila cyclic nucleotide gated channel pore mimicking NaK mutant Deposited 2015-04-14 | Assembly 2 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain B
20–110（91 aa）
Fragment：UNP residues 22-110
|
Not recorded | K POTASSIUM ION × 8 BA BARIUM ION × 4 | X-RAY DIFFRACTION |
X-ray crystallization conditions
EVAPORATION；293 K；65%MPD, 100mM KCl, 100mM MES, 4mM n-Decyl-beta-D-Maltoside
|
Resolution 1.90 Å R-free 0.236 |
| 6FIZ Crystal Structure of CNG mimicking NaK-EAPP mutant (T67A) cocrystallized with K+ Deposited 2018-01-19 | Assembly 1 Insufficient information Homooligomer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain A
20–64（45 aa）
Chain A
72–110（39 aa）
Chain B
20–64（45 aa）
Chain B
72–110（39 aa）
Chain C
20–64（45 aa）
Chain C
72–110（39 aa）
Chain D
20–64（45 aa）
Chain D
72–110（39 aa）
|
Not recorded | GLY GLYCINE × 10 K POTASSIUM ION × 3 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；277 K；40-44% MPD
100 mM MES pH 6.5
25mM Glycine
|
Resolution 2.63 Å R-free 0.269 |
| 6FIZ Crystal Structure of CNG mimicking NaK-EAPP mutant (T67A) cocrystallized with K+ Deposited 2018-01-19 | Assembly 2 Insufficient information Homooligomer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain E
20–64（45 aa）
Chain E
72–110（39 aa）
Chain F
20–64（45 aa）
Chain F
72–110（39 aa）
Chain G
20–64（45 aa）
Chain G
72–110（39 aa）
Chain H
20–64（45 aa）
Chain H
72–110（39 aa）
|
Not recorded | GLY GLYCINE × 12 K POTASSIUM ION × 3 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；277 K；40-44% MPD
100 mM MES pH 6.5
25mM Glycine
|
Resolution 2.63 Å R-free 0.269 |
| 6FIZ Crystal Structure of CNG mimicking NaK-EAPP mutant (T67A) cocrystallized with K+ Deposited 2018-01-19 | Assembly 3 Insufficient information Homooligomer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain I
20–64（45 aa）
Chain I
72–110（39 aa）
Chain J
20–64（45 aa）
Chain J
72–110（39 aa）
Chain K
20–64（45 aa）
Chain K
72–110（39 aa）
Chain L
20–64（45 aa）
Chain L
72–110（39 aa）
|
Not recorded | GLY GLYCINE × 17 K POTASSIUM ION × 3 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；277 K；40-44% MPD
100 mM MES pH 6.5
25mM Glycine
|
Resolution 2.63 Å R-free 0.269 |
| 6FIZ Crystal Structure of CNG mimicking NaK-EAPP mutant (T67A) cocrystallized with K+ Deposited 2018-01-19 | Assembly 4 Insufficient information Homooligomer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain M
20–64（45 aa）
Chain M
72–110（39 aa）
Chain N
20–64（45 aa）
Chain N
72–110（39 aa）
Chain O
20–64（45 aa）
Chain O
72–110（39 aa）
Chain P
20–64（45 aa）
Chain P
72–110（39 aa）
|
Not recorded | GLY GLYCINE × 21 K POTASSIUM ION × 3 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；277 K；40-44% MPD
100 mM MES pH 6.5
25mM Glycine
|
Resolution 2.63 Å R-free 0.269 |
| 7OOR NaK C-DI mutant with Na+ and K+ Deposited 2021-05-28 | Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain A
19–110（92 aa）
Chain B
19–110（92 aa）
|
Mutation：D66C G67- N68D F69I Mutation：D66C G67- N68D F69I | K POTASSIUM ION × 4 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 34 MRD (4R)-2-METHYLPENTANE-2,4-DIOL × 4 NA SODIUM ION × 10 CL CHLORIDE ION × 4 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.5；293 K；200mM KF, 40% MPD (2-Methyl-2,4-pentanediol racemate)
|
Resolution 1.47 Å R-free 0.183 |
| 7OOU NaK C-DI mutant with Li+ and K+ Deposited 2021-05-28 | Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain A
19–110（92 aa）
Chain B
19–110（92 aa）
|
Mutation：D66C G67- N68D F69I Mutation：D66C G67- N68D F69I | K POTASSIUM ION × 8 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 20 MRD (4R)-2-METHYLPENTANE-2,4-DIOL × 4 ACT ACETATE ION × 20 CL CHLORIDE ION × 4 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.5；293 K；200mM K acetate, 40% MPD (2-Methyl-2,4-pentanediol racemate)
|
Resolution 1.80 Å R-free 0.206 |
| 7OPH NaK S-DI mutant with Na+ and K+ Deposited 2021-05-31 | Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain A
19–110（92 aa）
Chain B
19–110（92 aa）
|
Mutation：D66S G67- N68D F69I Mutation：D66S G67- N68D F69I | K POTASSIUM ION × 12 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 22 CL CHLORIDE ION × 4 MRD (4R)-2-METHYLPENTANE-2,4-DIOL × 12 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；293 K；200 mM KCl, 100 mM HEPES (KOH) pH 7.5, 62% MPD (2-Methyl-2,4-pentanediol racemate)
|
Resolution 1.42 Å R-free 0.179 |
| 7OQ1 NaK S-ELM mutant with Na+ and K+ Deposited 2021-06-02 | Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain A
19–110（92 aa）
Chain B
19–110（92 aa）
|
Mutation：D66S G67- N68E F69L S70M Mutation：D66S G67- N68E F69L S70M | MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 14 K POTASSIUM ION × 6 ACT ACETATE ION × 24 CL CHLORIDE ION × 2 MRD (4R)-2-METHYLPENTANE-2,4-DIOL × 2 NA SODIUM ION × 4 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.5；293 K；100 mM HEPES (NaOH) pH 7.5, 47% MPD (2-Methyl-2,4-pentanediol racemate)
|
Resolution 1.85 Å R-free 0.186 |
| 7OQ2 NaK S-DI mutant soaked in Na+ Deposited 2021-06-02 | Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain A
19–110（92 aa）
Chain B
19–110（92 aa）
|
Mutation：D66S G67- N68D F69I Mutation：D66S G67- N68D F69I | MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 36 NA SODIUM ION × 8 K POTASSIUM ION × 4 CL CHLORIDE ION × 4 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；293 K；200 mM KCl, 100 mM HEPES (KOH) pH 7.5, 62% MPD (2-Methyl-2,4-pentanediol racemate) - soaked in 100 mM NaCl, 100 mM Tris-HCl pH 8.0, 50% MPD
|
Resolution 1.70 Å R-free 0.233 |
| 7PA0 NaK C-DI F92A mutant with Rb+ and K+ Deposited 2021-07-28 | Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain A
19–110（92 aa）
Chain B
19–110（92 aa）
|
Mutation：D66C G67- N68D F69I F92A Mutation：D66C G67- N68D F69I F92A | RB RUBIDIUM ION × 6 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 36 K POTASSIUM ION × 4 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8；293 K；200 mM RbCl, 100 mM Tris-HCl pH 8.0, 63% MPD (2-Methyl-2,4-pentanediol racemate)
|
Resolution 1.95 Å R-free 0.248 |
| 8A35 NaK C-DI mutant with Rb+ and Na+ Deposited 2022-06-07 | Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain A
19–110（92 aa）
|
Mutation：D66C G67- N68D F69I | RB RUBIDIUM ION × 8 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 8 NA SODIUM ION × 8 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.5；293 K；100 mM HEPES (NaOH) pH 7.5, 5% PEG 4000, 30% MPD (2-Methyl-2,4-pentanediol racemate)
|
Resolution 2.05 Å R-free 0.217 |
| 8A35 NaK C-DI mutant with Rb+ and Na+ Deposited 2022-06-07 | Assembly 2 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain B
19–110（92 aa）
|
Mutation：D66C G67- N68D F69I | RB RUBIDIUM ION × 8 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 20 NA SODIUM ION × 8 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.5；293 K；100 mM HEPES (NaOH) pH 7.5, 5% PEG 4000, 30% MPD (2-Methyl-2,4-pentanediol racemate)
|
Resolution 2.05 Å R-free 0.217 |
| 8A7X NaK C-DI F92A mutant soaked in Cs+ Deposited 2022-06-21 | Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain A
19–110（92 aa）
|
Mutation：D66C G67- N68D F69I F92A | CS CESIUM ION × 4 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 28 K POTASSIUM ION × 16 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8；293 K；200 mM CsCl 100 mM Tris-HCl pH 8.0, 63% MPD (2-Methyl-2,4-pentanediol racemate) - soaked in 90 mM CsCl, 100 mM Kryptofix-222, 50% MPD
|
Resolution 2.10 Å R-free 0.240 |
| 8A7X NaK C-DI F92A mutant soaked in Cs+ Deposited 2022-06-21 | Assembly 2 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain B
19–110（92 aa）
|
Mutation：D66C G67- N68D F69I F92A | CS CESIUM ION × 12 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 44 K POTASSIUM ION × 4 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8；293 K；200 mM CsCl 100 mM Tris-HCl pH 8.0, 63% MPD (2-Methyl-2,4-pentanediol racemate) - soaked in 90 mM CsCl, 100 mM Kryptofix-222, 50% MPD
|
Resolution 2.10 Å R-free 0.240 |
| 8AYP NaK C-DI mutant with Rb+ and Ba2+ Deposited 2022-09-02 | Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain A
19–110（92 aa）
Chain B
19–110（92 aa）
|
Not recorded | MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 24 RB RUBIDIUM ION × 4 BA BARIUM ION × 4 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 6；293 K；100 mM MES (NaOH), 40% v/v MPD (2-Methyl-2,4-pentanediol racemate)
|
Resolution 2.10 Å R-free 0.267 |
| 8AYP NaK C-DI mutant with Rb+ and Ba2+ Deposited 2022-09-02 | Assembly 2 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain C
19–110（92 aa）
Chain D
19–110（92 aa）
|
Not recorded | MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 34 RB RUBIDIUM ION × 4 BA BARIUM ION × 4 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 6；293 K；100 mM MES (NaOH), 40% v/v MPD (2-Methyl-2,4-pentanediol racemate)
|
Resolution 2.10 Å R-free 0.267 |
| 8AYQ NaK C-DI mutant with Rb+ and Ca2+ Deposited 2022-09-02 | Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain A
19–110（92 aa）
Chain B
19–110（92 aa）
|
Not recorded | RB RUBIDIUM ION × 4 CA CALCIUM ION × 6 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 22 CL CHLORIDE ION × 4 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 6；293 K；100 mM MES (NaOH), 40% v/v MPD (2-Methyl-2,4-pentanediol racemate)
|
Resolution 2.75 Å R-free 0.300 |
| 8AYQ NaK C-DI mutant with Rb+ and Ca2+ Deposited 2022-09-02 | Assembly 2 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain C
19–110（92 aa）
Chain D
19–110（92 aa）
|
Not recorded | RB RUBIDIUM ION × 4 CA CALCIUM ION × 2 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 14 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 6；293 K；100 mM MES (NaOH), 40% v/v MPD (2-Methyl-2,4-pentanediol racemate)
|
Resolution 2.75 Å R-free 0.300 |