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Protein Structures and Oligomeric States

Enter a protein name, UniProt accession or PDB ID. Name searches ask you to confirm the protein identity before related structures are queried.

Current Protein Identity：Q96P20 New Search
24PDB entries
25Biological assembly
106chain/assembly records
Main Difference Dimensions in This Set
Different construct Different mutation/modification Different assembly state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics

Difference tags compare only the current result set; every original PDB and assembly record remains separate.

Related-Structure Differences

Each row represents one biological assembly in one PDB entry; multiple monomers of the same protein are listed separately.

PDB Entry Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Experimental Method Experimental Conditions Structure Quality
2NAQ 3D NMR solution structure of NLRP3 PYD Deposited 2016-01-07 Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count
Chain A 3–93（91 aa） Fragment：Pyrin domain residues 3-93
Not recorded No recorded non-water small molecule SOLUTION NMR
NMR measurement conditions pH 3.6；303 K；Ionic strength (raw mmCIF value) 0.0051；Pressure ambient
NMR sample composition 100-200 uM [U-13C; U-15N] protein, 5 mM [U-2H] TCEP, 100 uM sodium azide, 95% H2O/5% D2O | 95% H2O/5% D2O
Resolution not provided
3QF2 Crystal structure of NALP3 PYD Deposited 2011-01-21 Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count
Chain A 3–112（110 aa） Fragment：PYD domain, DAPIN domain, residues 3-112
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 4.6；298 K；0.2M ammonium citrate, 0.1M sodium acetate pH4.6, 30% PEG MME 2000, VAPOR DIFFUSION, HANGING DROP, temperature 298K
Resolution 1.70 Å R-free 0.235
3QF2 Crystal structure of NALP3 PYD Deposited 2011-01-21 Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count
Chain B 3–112（110 aa） Fragment：PYD domain, DAPIN domain, residues 3-112
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 4.6；298 K；0.2M ammonium citrate, 0.1M sodium acetate pH4.6, 30% PEG MME 2000, VAPOR DIFFUSION, HANGING DROP, temperature 298K
Resolution 1.70 Å R-free 0.235
6NPY Cryo-EM structure of NLRP3 bound to NEK7 Deposited 2019-01-18 Assembly 1 Insufficient information Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count
Chain A 3–1036（1034 aa）
Mutation：D133I, R135C, K136A, K140A ADP ADENOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.80 Å
7ALV Crystal Structure of NLRP3 NACHT domain in complex with a potent inhibitor Deposited 2020-10-07 Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count
Chain A 131–679（549 aa） Fragment：NACHT domain
Not recorded RM5 1-[4-chloranyl-2,6-di(propan-2-yl)phenyl]-3-[4-(2-oxidanylpropan-2-yl)furan-2-yl]sulfonyl-urea × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.5；298 K；0.1 M Hepes pH 7.5, 1.4M SodiumCitrate
Resolution 2.83 Å R-free 0.265
7PZC Cryo-EM structure of the NLRP3 decamer bound to the inhibitor CRID3 Deposited 2021-10-12 Assembly 1 Protein homooligomer Homooligomer；Protein × 10 PDB declaration: decameric（10） Consistent with protein count
Chain A 1–1036（1036 aa）
Chain B 1–1036（1036 aa）
Chain C 1–1036（1036 aa）
Chain D 1–1036（1036 aa）
Chain E 1–1036（1036 aa）
Chain F 1–1036（1036 aa）
Chain G 1–1036（1036 aa）
Chain H 1–1036（1036 aa）
Chain I 1–1036（1036 aa）
Chain J 1–1036（1036 aa）
Not recorded ADP ADENOSINE-5'-DIPHOSPHATE × 10 8GI 1-(1,2,3,5,6,7-hexahydro-s-indacen-4-yl)-3-[4-(2-oxidanylpropan-2-yl)furan-2-yl]sulfonyl-urea × 10 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；50 mM Hepes pH 7.5, 150 mM NaCl, 0.5 mM TCEP, 10 mM MgCl2, 1 mM ADP
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.90 Å
7PZD Cryo-EM structure of the NLRP3 PYD filament Deposited 2021-10-12 Assembly 1 Protein homooligomer Homooligomer；Protein × 18 PDB declaration: octadecameric（18） Consistent with protein count
Chain G 3–110（108 aa）
Chain H 3–110（108 aa）
Chain I 3–110（108 aa）
Chain J 3–110（108 aa）
Chain K 3–110（108 aa）
Chain L 3–110（108 aa）
Chain M 3–110（108 aa）
Chain N 3–110（108 aa）
Chain O 3–110（108 aa）
Chain P 3–110（108 aa）
Chain Q 3–110（108 aa）
Chain R 3–110（108 aa）
Chain S 3–110（108 aa）
Chain T 3–110（108 aa）
Chain U 3–110（108 aa）
Chain V 3–110（108 aa）
Chain W 3–110（108 aa）
Chain X 3–110（108 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM HEPES (pH 7.5), 150 mM NaCl, 0.5 mM TCEP
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
7VTP Cryo-EM structure of PYD-deleted human NLRP3 hexamer Deposited 2021-10-30 Assembly 1 Protein homooligomer Homooligomer；Protein × 6 PDB declaration: hexameric（6） Consistent with protein count
Chain A 130–1036（907 aa）
Chain B 130–1036（907 aa）
Chain C 130–1036（907 aa）
Chain D 130–1036（907 aa）
Chain E 130–1036（907 aa）
Chain F 130–1036（907 aa）
Not recorded ADP ADENOSINE-5'-DIPHOSPHATE × 6 7YN 1-[4-(2-oxidanylpropan-2-yl)furan-2-yl]sulfonyl-3-(1,2,3,5-tetrahydro-s-indacen-4-yl)urea × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；25 mM HEPES-NaOH (pH 7.5), 0.2 M NaCl, 1 mM MgCl2, 0.5 mM TCEP, 1.0 mM ADP, 0.05 mM MCC950
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.23 Å
7ZGU Human NLRP3-deltaPYD hexamer Deposited 2022-04-04 Assembly 1 Protein homooligomer Homooligomer；Protein × 6 PDB declaration: hexameric（6） Consistent with protein count
Chain A 126–1036（911 aa）
Chain B 126–1036（911 aa）
Chain C 126–1036（911 aa）
Chain D 126–1036（911 aa）
Chain E 126–1036（911 aa）
Chain F 126–1036（911 aa）
Not recorded ADP ADENOSINE-5'-DIPHOSPHATE × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
8EJ4 Cryo-EM structure of the active NLRP3 inflammasome disk Deposited 2022-09-16 Assembly 1 Protein heterocomplex Heteromer；Protein × 20 PDB declaration: eicosameric（20） Consistent with protein count
Chain A 133–1034（902 aa）
Chain B 133–1034（902 aa）
Chain C 133–1034（902 aa）
Chain D 133–1034（902 aa）
Chain E 133–1034（902 aa）
Chain F 133–1034（902 aa）
Chain G 133–1034（902 aa）
Chain H 133–1034（902 aa）
Chain I 133–1034（902 aa）
Chain J 133–1034（902 aa）
Not recorded AGS PHOSPHOTHIOPHOSPHORIC ACID-ADENYLATE ESTER × 10 MG MAGNESIUM ION × 10 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
8ERT NLRP3 PYD filament Deposited 2022-10-12 Assembly 1 Protein homooligomer Homooligomer；Protein × 21 PDB declaration: 21-meric（21） Consistent with protein count
Chain A 1–95（95 aa）
Chain B 1–95（95 aa）
Chain C 1–95（95 aa）
Chain D 1–95（95 aa）
Chain E 1–95（95 aa）
Chain F 1–95（95 aa）
Chain G 1–95（95 aa）
Chain I 1–95（95 aa）
Chain J 1–95（95 aa）
Chain K 1–95（95 aa）
Chain L 1–95（95 aa）
Chain M 1–95（95 aa）
Chain N 1–95（95 aa）
Chain O 1–95（95 aa）
Chain Q 1–95（95 aa）
Chain R 1–95（95 aa）
Chain S 1–95（95 aa）
Chain U 1–95（95 aa）
Chain V 1–95（95 aa）
Chain W 1–95（95 aa）
Chain X 1–95（95 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8ETR CryoEM Structure of NLRP3 NACHT domain in complex with G2394 Deposited 2022-10-17 Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count
Chain A 134–676（543 aa）
Not recorded MG MAGNESIUM ION × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 1 WTN (6S,8R)-N-[(1,2,3,5,6,7-hexahydro-s-indacen-4-yl)carbamoyl]-6-(methylamino)-6,7-dihydro-5H-pyrazolo[5,1-b][1,3]oxazine-3-sulfonamide × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；0.15M NaCl, 20mM Tris pH 7.5, 10% glycerol, 1mM TCEP, 2.5mM ATP, 2mM MgCl2.
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.50 Å
8RI2 Crystal structure of NLRP3 in complex with inhibitor NP3-562 Deposited 2023-12-18 Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count
Chain A 131–679（549 aa）
Not recorded A1H02 2-[4-chloranyl-9-oxidanylidene-12-(2-oxidanylpropan-2-yl)-5-thia-1,10,11-triazatricyclo[6.4.0.0^{2,6}]dodeca-2(6),3,7,11-tetraen-10-yl]-~{N}-[(3~{R})-1-methylpiperidin-3-yl]ethanamide × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；298 K；protein: well = 1: 1 well: 1.94 M Ammonium citrate pH 7.0 protein: 7 mg/ml protein copurified with 1 uM inhibitor
Resolution 2.80 Å R-free 0.270
8SWF Cryo-EM structure of NLRP3 open octamer Deposited 2023-05-18 Assembly 1 Protein homooligomer Homooligomer；Protein × 8 PDB declaration: octameric（8） Consistent with protein count
Chain A 130–1036（907 aa）
Chain B 130–1036（907 aa）
Chain C 130–1036（907 aa）
Chain D 130–1036（907 aa）
Chain E 130–1036（907 aa）
Chain F 130–1036（907 aa）
Chain G 130–1036（907 aa）
Chain H 130–1036（907 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.39 Å
8SWK Cryo-EM structure of NLRP3 closed hexamer Deposited 2023-05-18 Assembly 1 Protein homooligomer Homooligomer；Protein × 6 PDB declaration: hexameric（6） Consistent with protein count
Chain A 133–1036（904 aa）
Chain B 133–1036（904 aa）
Chain C 133–1036（904 aa）
Chain D 133–1036（904 aa）
Chain E 133–1036（904 aa）
Chain F 133–1036（904 aa）
Not recorded 7YN 1-[4-(2-oxidanylpropan-2-yl)furan-2-yl]sulfonyl-3-(1,2,3,5-tetrahydro-s-indacen-4-yl)urea × 6 ATP ADENOSINE-5'-TRIPHOSPHATE × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.32 Å
8SXN Structure of NLRP3 and NEK7 complex Deposited 2023-05-22 Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count
Chain C 133–1036（904 aa）
Chain D 133–1036（904 aa）
Not recorded 7YN 1-[4-(2-oxidanylpropan-2-yl)furan-2-yl]sulfonyl-3-(1,2,3,5-tetrahydro-s-indacen-4-yl)urea × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.04 Å
8WSM NLRP3 NACHT domain in complex with compound 32 Deposited 2023-10-17 Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count
Chain A 131–679（549 aa）
Not recorded MG MAGNESIUM ION × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 1 XE3 2-[[2-methyl-5-(trifluoromethyl)phenyl]amino]-~{N}-(1,4-oxazepan-4-ylsulfonyl)-1,3-oxazole-4-carboxamide × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；298 K；0.1 M Tris pH 7.5, 1.2 M sodium citrate
Resolution 2.70 Å R-free 0.248
8ZEM Crystal Structure of NLRP3 NACHT domain in complex with NP3-1 Deposited 2024-05-06 Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count
Chain A 131–679（549 aa）
Not recorded A1D79 1-[5-[2,3-bis(chloranyl)phenyl]-2,3-dihydro-1~{H}-inden-4-yl]-3-[4-(2-oxidanylpropan-2-yl)thiophen-2-yl]sulfonyl-urea × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；291 K；23~25 % w/v Polyethylene glycol monomethyl ether 5,000 ;100 mM MES pH 6.3~6.7 ;200 mM Ammonium sulfate
Resolution 3.32 Å R-free 0.347
9DH3 Cryo-EM structure of NLRP3 complex with Compound C Deposited 2024-09-03 Assembly 1 Insufficient information Homooligomer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count
Chain A 136–1036（901 aa）
Chain B 136–1036（901 aa）
Chain C 136–1036（901 aa）
Chain D 136–1036（901 aa）
Not recorded ADP ADENOSINE-5'-DIPHOSPHATE × 4 A1A4L 2-[(4S)-5-ethyl-8-oxothieno[2',3':4,5]pyrrolo[1,2-d][1,2,4]triazin-7(8H)-yl]-N-(pyrimidin-4-yl)acetamide × 4 CPS 3-[(3-CHOLAMIDOPROPYL)DIMETHYLAMMONIO]-1-PROPANESULFONATE × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.76 Å
9GU4 Crystal structure of NLRP3 in complex with inhibitor NP3-253 Deposited 2024-09-18 Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count
Chain A 131–679（549 aa）
Not recorded A1IPJ 2-[6-[[(3R)-1-ethylpiperidin-3-yl]amino]pyridazin-3-yl]-3-methyl-5-(trifluoromethyl)phenol × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；298 K；protein: well = 1: 1 well: 1.94 M Ammonium citrate pH 7.0 protein: 7 mg/ml, 1mM inhibitor co-complex
Resolution 2.70 Å R-free 0.264
9HG4 NACHT domain of NLRP3 in complex with DFV890 Deposited 2024-11-18 Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count
Chain A 131–679（549 aa）
Not recorded A1IUG 1-[azanyl-oxidanylidene-[2-(2-oxidanylpropan-2-yl)-1,3-thiazol-5-yl]-$l^{6}-sulfanylidene]-3-(1,2,3,5,6,7-hexahydro-s-indacen-4-yl)urea × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.8；293 K；protein: 0.2 ul NLRP3 (131-679) at 7 mg/ml in 50mM HEPES pH 7.8, 500mM NaCl, 10% glycerol, 1mM MgCl2, 1mM TCEP, 100uM ADP, and 0.8 mM DFV890 (in DMSO) wel: 0.2 ul 1.99M NH4 3 citrate
Resolution 2.77 Å R-free 0.264
9MGY Cryo-EM structure of Human NLRP3 complex with compound 1 Deposited 2024-12-11 Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count
Chain C 1–1036（1036 aa）
Not recorded ATP ADENOSINE-5'-TRIPHOSPHATE × 1 A1BLR (2M)-2-(6-{[(3R)-1-methylpiperidin-3-yl]amino}pyridazin-3-yl)-5-(trifluoromethyl)phenol × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
9MIE Human NLRP3 complex with compound 2 in the closed hexamer Deposited 2024-12-12 Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count
Chain C 1–1036（1036 aa）
Not recorded ATP ADENOSINE-5'-TRIPHOSPHATE × 1 A1BLP (2P)-2-(4-{[(3R)-1-methylpiperidin-3-yl]amino}-6,7-dihydro-5H-cyclopenta[d]pyridazin-1-yl)-5-(trifluoromethyl)phenol × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.93 Å
9MIG Cryo-EM structure of Human NLRP3 complex with compound 3 Deposited 2024-12-12 Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count
Chain C 1–1036（1036 aa）
Not recorded ATP ADENOSINE-5'-TRIPHOSPHATE × 1 A1BLQ (2P)-2-(4-{[(3R)-1-methylpiperidin-3-yl]amino}-5,6,7,8-tetrahydrophthalazin-1-yl)-5-(trifluoromethyl)phenol × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
9SFG Crystal structure of NLRP3 in complex with inhibitor NP3-742 Deposited 2025-08-19 Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count
Chain A 131–679（549 aa）
Not recorded A1JNK 5-methyl-~{N}-[(3~{R})-1-methylpiperidin-3-yl]-6-(2-methyl-1~{H}-pyrrolo[2,3-b]pyridin-6-yl)pyridazin-3-amine × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；298 K；protein: well = 1: 1 well: 1.94 M Ammonium citrate pH 7.0 protein: 7 mg/ml, 1mM inhibitor co-complex
Resolution 3.20 Å R-free 0.279

This page presents structural evidence recorded in PDB/mmCIF.“No recorded non-water small molecule” does not prove that the experimental sample was ligand-free; experimental conditions belong to the PDB experiment, while ligands and copy counts are shown for each biological assembly.