当前蛋白身份：Q9Y3D9
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差异标签只比较当前检索结果；所有PDB和assembly原始记录仍分别保留。
相关结构差异明细
一行代表一个 PDB 条目中的一个 biological assembly；同一蛋白的多个单体会分别列出。
| PDB 条目 | Assembly / 聚集状态 | 构建体 | 突变与修饰 | 配体、离子与非聚合物 | 实验方法 | 实验环境 | 结构质量 |
|---|---|---|---|---|---|---|---|
| 3J9M Structure of the human mitochondrial ribosome (class 1) 提交 2015-02-08 | Assembly 1 蛋白–RNA 异源复合物；蛋白 × 81 PDB 声明：85-meric（85） 与全部聚合物一致 |
链 AS
1–190（190 aa）
|
未记录 | MG MAGNESIUM ION × 127 ZN ZINC ION × 7 GDP GUANOSINE-5'-DIPHOSPHATE × 1 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
20 mM HEPES-KOH, pH 7.45, 100 mM KCl, 20 mM MgOAc, 2 mM DTT；pH 7.45；20 mM HEPES-KOH, pH 7.45, 100 mM KCl, 20 mM MgOAc, 2 mM DTT
cryo-EM玻璃化条件
Blot 2.5 seconds before plunging；90 K；冷冻剂 ETHANE；Blot 2.5 seconds before plunging into liquid ethane (FEI VITROBOT MARK II).
|
分辨率 3.50 Å |
| 6NU2 Structural insights into unique features of the human mitochondrial ribosome recycling 提交 2019-01-30 | Assembly 1 蛋白–RNA 异源复合物；蛋白 × 82 PDB 声明：86-meric（86） 与全部聚合物一致 |
链 AS
2–127（126 aa）
|
未记录 | MG MAGNESIUM ION × 127 ZN ZINC ION × 7 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 7.6
cryo-EM玻璃化条件
冷冻剂 ETHANE
|
分辨率 3.90 Å |
| 6NU3 Structural insights into unique features of the human mitochondrial ribosome recycling 提交 2019-01-30 | Assembly 1 蛋白–RNA 异源复合物；蛋白 × 81 PDB 声明：85-meric（85） 与全部聚合物一致 |
链 AS
1–190（190 aa）
|
未记录 | MG MAGNESIUM ION × 127 ZN ZINC ION × 7 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 7.6
cryo-EM玻璃化条件
冷冻剂 ETHANE
|
分辨率 4.40 Å |
| 6RW4 Structure of human mitochondrial 28S ribosome in complex with mitochondrial IF3 提交 2019-06-03 | Assembly 1 蛋白–RNA 异源复合物；蛋白 × 31 PDB 声明：32-meric（32） 与全部聚合物一致 |
链 S
1–190（190 aa）
|
未记录 | NAD NICOTINAMIDE-ADENINE-DINUCLEOTIDE × 1 SPM SPERMINE × 1 SRY STREPTOMYCIN × 1 MG MAGNESIUM ION × 60 K POTASSIUM ION × 17 ZN ZINC ION × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 7.5
cryo-EM玻璃化条件
冷冻剂 ETHANE
|
分辨率 2.97 Å |
| 6RW5 Structure of human mitochondrial 28S ribosome in complex with mitochondrial IF2 and IF3 提交 2019-06-03 | Assembly 1 蛋白–RNA 异源复合物；蛋白 × 32 PDB 声明：33-meric（33） 与全部聚合物一致 |
链 S
1–190（190 aa）
|
未记录 | NAD NICOTINAMIDE-ADENINE-DINUCLEOTIDE × 1 SPM SPERMINE × 1 SRY STREPTOMYCIN × 1 MG MAGNESIUM ION × 61 K POTASSIUM ION × 17 ZN ZINC ION × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 2 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 7.5
cryo-EM玻璃化条件
冷冻剂 ETHANE
|
分辨率 3.14 Å |
| 6VLZ Structure of the human mitochondrial ribosome-EF-G1 complex (ClassI) 提交 2020-01-27 | Assembly 1 其他组合 异源复合物；蛋白 × 85 PDB 声明：90-meric（90） 与全部聚合物一致 |
链 AS
1–190（190 aa）
|
未记录 | MG MAGNESIUM ION × 130 ZN ZINC ION × 7 GDP GUANOSINE-5'-DIPHOSPHATE × 1 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 7.5
cryo-EM玻璃化条件
冷冻剂 ETHANE
|
分辨率 2.97 Å |
| 6VMI Structure of the human mitochondrial ribosome-EF-G1 complex (ClassIII) 提交 2020-01-28 | Assembly 1 其他组合 异源复合物；蛋白 × 85 PDB 声明：91-meric（91） 与全部聚合物一致 |
链 AS
1–190（190 aa）
|
未记录 | MG MAGNESIUM ION × 130 ZN ZINC ION × 7 GDP GUANOSINE-5'-DIPHOSPHATE × 1 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 7.5
cryo-EM玻璃化条件
冷冻剂 ETHANE
|
分辨率 2.96 Å |
| 6ZM5 Human mitochondrial ribosome in complex with OXA1L, mRNA, A/A tRNA, P/P tRNA and nascent polypeptide 提交 2020-07-01 | Assembly 1 蛋白–RNA 异源复合物；蛋白 × 88 PDB 声明：94-meric（94） 与全部聚合物一致 |
链 AS
1–190（190 aa）
|
未记录 | MG MAGNESIUM ION × 189 K POTASSIUM ION × 44 ZN ZINC ION × 3 FES FE2/S2 (INORGANIC) CLUSTER × 3 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ALA ALANINE × 1 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 7.5
cryo-EM玻璃化条件
冷冻剂 ETHANE
|
分辨率 2.89 Å |
| 6ZM6 Human mitochondrial ribosome in complex with mRNA, A/A tRNA and P/P tRNA 提交 2020-07-01 | Assembly 1 蛋白–RNA 异源复合物；蛋白 × 86 PDB 声明：92-meric（92） 与全部聚合物一致 |
链 AS
1–190（190 aa）
|
未记录 | MG MAGNESIUM ION × 189 K POTASSIUM ION × 44 ZN ZINC ION × 3 FES FE2/S2 (INORGANIC) CLUSTER × 3 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 7.5
cryo-EM玻璃化条件
冷冻剂 ETHANE
|
分辨率 2.59 Å |
| 6ZS9 Human mitochondrial ribosome in complex with ribosome recycling factor 提交 2020-07-15 | Assembly 1 蛋白–RNA 异源复合物；蛋白 × 88 PDB 声明：91-meric（91） 与全部聚合物一致 |
链 AS
1–190（190 aa）
|
未记录 | ZN ZINC ION × 7 MG MAGNESIUM ION × 195 DOL 5-(2-DIETHYLAMINO-ETHANESULFONYL)-21-HYDROXY-10-ISOPROPYL-11,19-DIMETHYL-9,26-DIOXA-3,15,28-TRIAZA-TRICYCLO[23.2.1.00,255]OCTACOSA-1(27),12,17,19,25(28)-PENTAENE-2,8,14,23-TETRAONE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 7.5
cryo-EM玻璃化条件
冷冻剂 ETHANE
|
分辨率 4.00 Å |
| 6ZSA Human mitochondrial ribosome bound to mRNA, A-site tRNA and P-site tRNA 提交 2020-07-15 | Assembly 1 蛋白–RNA 异源复合物；蛋白 × 86 PDB 声明：92-meric（92） 与全部聚合物一致 |
链 AS
1–190（190 aa）
|
未记录 | ZN ZINC ION × 7 MG MAGNESIUM ION × 195 H8Q ~{N}-[(3~{S},6~{R},12~{R},15~{S},16~{S},19~{S},22~{S},25~{S})-25-[[(3~{S})-1-azabicyclo[2.2.2]octan-3-yl]sulfanylmethyl]-3-[[4-(dimethylamino)phenyl]methyl]-12-ethyl-4,16-dimethyl-2,5,11,14,18,21,24-heptakis(oxidanylidene)-19-phenyl-17-oxa-1,4,10,13,20-pentazatricyclo[20.4.0.0^{6,10}]hexacosan-15-yl]-3-oxidanyl-pyridine-2-carboxamide × 1 DOL 5-(2-DIETHYLAMINO-ETHANESULFONYL)-21-HYDROXY-10-ISOPROPYL-11,19-DIMETHYL-9,26-DIOXA-3,15,28-TRIAZA-TRICYCLO[23.2.1.00,255]OCTACOSA-1(27),12,17,19,25(28)-PENTAENE-2,8,14,23-TETRAONE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 7.5
cryo-EM玻璃化条件
冷冻剂 ETHANE
|
分辨率 4.00 Å |
| 6ZSB Human mitochondrial ribosome in complex with mRNA and P-site tRNA 提交 2020-07-15 | Assembly 1 蛋白–RNA 异源复合物；蛋白 × 87 PDB 声明：92-meric（92） 与全部聚合物一致 |
链 AS
1–190（190 aa）
|
未记录 | ZN ZINC ION × 7 MG MAGNESIUM ION × 195 DOL 5-(2-DIETHYLAMINO-ETHANESULFONYL)-21-HYDROXY-10-ISOPROPYL-11,19-DIMETHYL-9,26-DIOXA-3,15,28-TRIAZA-TRICYCLO[23.2.1.00,255]OCTACOSA-1(27),12,17,19,25(28)-PENTAENE-2,8,14,23-TETRAONE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 7.5
cryo-EM玻璃化条件
冷冻剂 ETHANE
|
分辨率 4.50 Å |
| 6ZSC Human mitochondrial ribosome in complex with E-site tRNA 提交 2020-07-15 | Assembly 1 蛋白–RNA 异源复合物；蛋白 × 87 PDB 声明：91-meric（91） 与全部聚合物一致 |
链 AS
1–190（190 aa）
|
未记录 | ZN ZINC ION × 7 MG MAGNESIUM ION × 195 DOL 5-(2-DIETHYLAMINO-ETHANESULFONYL)-21-HYDROXY-10-ISOPROPYL-11,19-DIMETHYL-9,26-DIOXA-3,15,28-TRIAZA-TRICYCLO[23.2.1.00,255]OCTACOSA-1(27),12,17,19,25(28)-PENTAENE-2,8,14,23-TETRAONE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 7.5
cryo-EM玻璃化条件
冷冻剂 ETHANE
|
分辨率 3.50 Å |
| 6ZSD Human mitochondrial ribosome in complex with mRNA, P-site tRNA and E-site tRNA 提交 2020-07-15 | Assembly 1 蛋白–RNA 异源复合物；蛋白 × 87 PDB 声明：93-meric（93） 与全部聚合物一致 |
链 AS
1–190（190 aa）
|
未记录 | ZN ZINC ION × 7 MG MAGNESIUM ION × 195 DOL 5-(2-DIETHYLAMINO-ETHANESULFONYL)-21-HYDROXY-10-ISOPROPYL-11,19-DIMETHYL-9,26-DIOXA-3,15,28-TRIAZA-TRICYCLO[23.2.1.00,255]OCTACOSA-1(27),12,17,19,25(28)-PENTAENE-2,8,14,23-TETRAONE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 7.5
cryo-EM玻璃化条件
冷冻剂 ETHANE
|
分辨率 3.70 Å |
| 6ZSE Human mitochondrial ribosome in complex with mRNA, A/P-tRNA and P/E-tRNA 提交 2020-07-15 | Assembly 1 蛋白–RNA 异源复合物；蛋白 × 87 PDB 声明：93-meric（93） 与全部聚合物一致 |
链 AS
1–190（190 aa）
|
未记录 | ZN ZINC ION × 7 MG MAGNESIUM ION × 195 DOL 5-(2-DIETHYLAMINO-ETHANESULFONYL)-21-HYDROXY-10-ISOPROPYL-11,19-DIMETHYL-9,26-DIOXA-3,15,28-TRIAZA-TRICYCLO[23.2.1.00,255]OCTACOSA-1(27),12,17,19,25(28)-PENTAENE-2,8,14,23-TETRAONE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 7.5
cryo-EM玻璃化条件
冷冻剂 ETHANE
|
分辨率 5.00 Å |
| 6ZSG Human mitochondrial ribosome in complex with mRNA, A-site tRNA, P-site tRNA and E-site tRNA 提交 2020-07-15 | Assembly 1 蛋白–RNA 异源复合物；蛋白 × 87 PDB 声明：94-meric（94） 与全部聚合物一致 |
链 AS
1–190（190 aa）
|
未记录 | ZN ZINC ION × 7 MG MAGNESIUM ION × 195 DOL 5-(2-DIETHYLAMINO-ETHANESULFONYL)-21-HYDROXY-10-ISOPROPYL-11,19-DIMETHYL-9,26-DIOXA-3,15,28-TRIAZA-TRICYCLO[23.2.1.00,255]OCTACOSA-1(27),12,17,19,25(28)-PENTAENE-2,8,14,23-TETRAONE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 7.5
cryo-EM玻璃化条件
冷冻剂 ETHANE
|
分辨率 4.00 Å |
| 7A5F Structure of the stalled human mitoribosome with P- and E-site mt-tRNAs 提交 2020-08-21 | Assembly 1 蛋白–RNA 异源复合物；蛋白 × 85 PDB 声明：91-meric（91） 与全部聚合物一致 |
链 S6
1–190（190 aa）
|
未记录 | MG MAGNESIUM ION × 128 ZN ZINC ION × 7 GDP GUANOSINE-5'-DIPHOSPHATE × 1 SO4 SULFATE ION × 1 MLI MALONATE ION × 3 CL CHLORIDE ION × 2 NA SODIUM ION × 4 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 7.4
cryo-EM玻璃化条件
冷冻剂 ETHANE
|
分辨率 4.40 Å |
| 7A5G Structure of the elongating human mitoribosome bound to mtEF-Tu.GMPPCP and A/T mt-tRNA 提交 2020-08-21 | Assembly 1 蛋白–RNA 异源复合物；蛋白 × 86 PDB 声明：94-meric（94） 与全部聚合物一致 |
链 S6
1–190（190 aa）
|
未记录 | MG MAGNESIUM ION × 128 SPD SPERMIDINE × 1 ZN ZINC ION × 7 GDP GUANOSINE-5'-DIPHOSPHATE × 1 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 NA SODIUM ION × 4 SO4 SULFATE ION × 1 MLI MALONATE ION × 3 CL CHLORIDE ION × 2 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 7.4
cryo-EM玻璃化条件
冷冻剂 ETHANE
|
分辨率 4.33 Å |
| 7A5I Structure of the human mitoribosome with A- P-and E-site mt-tRNAs 提交 2020-08-21 | Assembly 1 蛋白–RNA 异源复合物；蛋白 × 83 PDB 声明：90-meric（90） 与全部聚合物一致 |
链 S6
1–190（190 aa）
|
未记录 | MG MAGNESIUM ION × 127 ZN ZINC ION × 7 CL CHLORIDE ION × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 7.4
cryo-EM玻璃化条件
冷冻剂 ETHANE
|
分辨率 3.70 Å |
| 7A5K Structure of the human mitoribosome in the post translocation state bound to mtEF-G1 提交 2020-08-21 | Assembly 1 蛋白–RNA 异源复合物；蛋白 × 86 PDB 声明：93-meric（93） 与全部聚合物一致 |
链 S6
1–190（190 aa）
|
未记录 | GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 MG MAGNESIUM ION × 128 SPD SPERMIDINE × 1 ZN ZINC ION × 7 GDP GUANOSINE-5'-DIPHOSPHATE × 1 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 7.4
cryo-EM玻璃化条件
冷冻剂 ETHANE
|
分辨率 3.70 Å |
| 7L08 Cryo-EM structure of the human 55S mitoribosome-RRFmt complex. 提交 2020-12-11 | Assembly 1 蛋白–RNA 异源复合物；蛋白 × 84 PDB 声明：87-meric（87） 与全部聚合物一致 |
链 AS
1–190（190 aa）
|
未记录 | MG MAGNESIUM ION × 127 ZN ZINC ION × 7 GDP GUANOSINE-5'-DIPHOSPHATE × 1 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 7.5
cryo-EM玻璃化条件
冷冻剂 ETHANE
|
分辨率 3.49 Å |
| 7OG4 Human mitochondrial ribosome in complex with P/E-tRNA 提交 2021-05-06 | Assembly 1 蛋白–RNA 异源复合物；蛋白 × 86 PDB 声明：90-meric（90） 与全部聚合物一致 |
链 AS
1–190（190 aa）
|
未记录 | ZN ZINC ION × 7 MG MAGNESIUM ION × 195 H8Q ~{N}-[(3~{S},6~{R},12~{R},15~{S},16~{S},19~{S},22~{S},25~{S})-25-[[(3~{S})-1-azabicyclo[2.2.2]octan-3-yl]sulfanylmethyl]-3-[[4-(dimethylamino)phenyl]methyl]-12-ethyl-4,16-dimethyl-2,5,11,14,18,21,24-heptakis(oxidanylidene)-19-phenyl-17-oxa-1,4,10,13,20-pentazatricyclo[20.4.0.0^{6,10}]hexacosan-15-yl]-3-oxidanyl-pyridine-2-carboxamide × 1 DOL 5-(2-DIETHYLAMINO-ETHANESULFONYL)-21-HYDROXY-10-ISOPROPYL-11,19-DIMETHYL-9,26-DIOXA-3,15,28-TRIAZA-TRICYCLO[23.2.1.00,255]OCTACOSA-1(27),12,17,19,25(28)-PENTAENE-2,8,14,23-TETRAONE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 7.5
cryo-EM玻璃化条件
冷冻剂 ETHANE
|
分辨率 3.80 Å |
| 7P2E Human mitochondrial ribosome small subunit in complex with IF3, GMPPMP and streptomycin 提交 2021-07-05 | Assembly 1 蛋白–RNA 异源复合物；蛋白 × 31 PDB 声明：32-meric（32） 与全部聚合物一致 |
链 S
1–190（190 aa）
|
未记录 | NAD NICOTINAMIDE-ADENINE-DINUCLEOTIDE × 1 SPM SPERMINE × 1 5I0 [(2~{S},3~{S},4~{S},5~{R},6~{S})-2-[(2~{R},3~{R},4~{R},5~{S})-2-[(1~{R},2~{S},3~{R},4~{R},5~{S},6~{R})-2,4-bis[[azaniumylidene(azanyl)methyl]amino]-3,5,6-tris(oxidanyl)cyclohexyl]oxy-4-[bis(oxidanyl)methyl]-5-methyl-4-oxidanyl-oxolan-3-yl]oxy-6-(hydroxymethyl)-4,5-bis(oxidanyl)oxan-3-yl]-methyl-azanium × 1 K POTASSIUM ION × 21 MG MAGNESIUM ION × 62 ZN ZINC ION × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 7.5
cryo-EM玻璃化条件
冷冻剂 ETHANE
|
分辨率 2.40 Å |
| 7PNX Assembly intermediate of human mitochondrial ribosome small subunit without mS37 in complex with RBFA and METTL15 conformation a 提交 2021-09-08 | Assembly 1 蛋白–RNA 异源复合物；蛋白 × 31 PDB 声明：32-meric（32） 与全部聚合物一致 |
链 S
1–190（190 aa）
|
未记录 | NAD NICOTINAMIDE-ADENINE-DINUCLEOTIDE × 1 MG MAGNESIUM ION × 64 K POTASSIUM ION × 16 ZN ZINC ION × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 SAH S-ADENOSYL-L-HOMOCYSTEINE × 1 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 7.5
cryo-EM玻璃化条件
冷冻剂 ETHANE
|
分辨率 2.76 Å |
| 7PNY Assembly intermediate of human mitochondrial ribosome small subunit without mS37 in complex with RBFA and METTL15 conformation b 提交 2021-09-08 | Assembly 1 蛋白–RNA 异源复合物；蛋白 × 31 PDB 声明：32-meric（32） 与全部聚合物一致 |
链 S
1–190（190 aa）
|
未记录 | NAD NICOTINAMIDE-ADENINE-DINUCLEOTIDE × 1 MG MAGNESIUM ION × 61 K POTASSIUM ION × 20 ZN ZINC ION × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 SAH S-ADENOSYL-L-HOMOCYSTEINE × 1 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 7.5
cryo-EM玻璃化条件
冷冻剂 ETHANE
|
分辨率 3.06 Å |
| 7PNZ Assembly intermediate of human mitochondrial ribosome small subunit without mS37 in complex with RBFA and METTL15 conformation c 提交 2021-09-08 | Assembly 1 蛋白–RNA 异源复合物；蛋白 × 31 PDB 声明：32-meric（32） 与全部聚合物一致 |
链 S
1–190（190 aa）
|
未记录 | NAD NICOTINAMIDE-ADENINE-DINUCLEOTIDE × 1 MG MAGNESIUM ION × 61 K POTASSIUM ION × 17 ZN ZINC ION × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 SAH S-ADENOSYL-L-HOMOCYSTEINE × 1 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 7.5
cryo-EM玻璃化条件
冷冻剂 ETHANE
|
分辨率 3.09 Å |
| 7PO0 Assembly intermediate of human mitochondrial ribosome small subunit without mS37 in complex with RBFA and IF3 提交 2021-09-08 | Assembly 1 蛋白–RNA 异源复合物；蛋白 × 31 PDB 声明：32-meric（32） 与全部聚合物一致 |
链 S
1–190（190 aa）
|
未记录 | NAD NICOTINAMIDE-ADENINE-DINUCLEOTIDE × 1 MG MAGNESIUM ION × 65 K POTASSIUM ION × 17 ZN ZINC ION × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 7.5
cryo-EM玻璃化条件
冷冻剂 ETHANE
|
分辨率 2.90 Å |
| 7PO1 Initiation complex of human mitochondrial ribosome small subunit with IF3 提交 2021-09-08 | Assembly 1 蛋白–RNA 异源复合物；蛋白 × 31 PDB 声明：32-meric（32） 与全部聚合物一致 |
链 S
1–190（190 aa）
|
未记录 | MG MAGNESIUM ION × 71 K POTASSIUM ION × 20 ZN ZINC ION × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 7.5
cryo-EM玻璃化条件
冷冻剂 ETHANE
|
分辨率 2.92 Å |
| 7PO2 Initiation complex of human mitochondrial ribosome small subunit with IF2, fMet-tRNAMet and mRNA 提交 2021-09-08 | Assembly 1 蛋白–RNA 异源复合物；蛋白 × 32 PDB 声明：35-meric（35） 与全部聚合物一致 |
链 S
1–190（190 aa）
|
未记录 | MG MAGNESIUM ION × 72 K POTASSIUM ION × 20 ZN ZINC ION × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 FME N-FORMYLMETHIONINE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 7.5
cryo-EM玻璃化条件
冷冻剂 ETHANE
|
分辨率 3.09 Å |
| 7PO3 Human mitochondrial ribosome small subunit 提交 2021-09-08 | Assembly 1 蛋白–RNA 异源复合物；蛋白 × 30 PDB 声明：31-meric（31） 与全部聚合物一致 |
链 S
1–190（190 aa）
|
未记录 | MG MAGNESIUM ION × 64 K POTASSIUM ION × 21 ZN ZINC ION × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 7.5
cryo-EM玻璃化条件
冷冻剂 ETHANE
|
分辨率 2.92 Å |
| 7QI4 Human mitochondrial ribosome at 2.2 A resolution (bound to partly built tRNAs and mRNA) 提交 2021-12-14 | Assembly 1 蛋白–RNA 异源复合物；蛋白 × 87 PDB 声明：94-meric（94） 与全部聚合物一致 |
链 AS
1–190（190 aa）
|
未记录 | NAD NICOTINAMIDE-ADENINE-DINUCLEOTIDE × 1 SPM SPERMINE × 1 SPD SPERMIDINE × 4 MG MAGNESIUM ION × 206 K POTASSIUM ION × 54 ZN ZINC ION × 3 FES FE2/S2 (INORGANIC) CLUSTER × 3 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 PUT 1,4-DIAMINOBUTANE × 1 VAL VALINE × 1 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 7.5
cryo-EM玻璃化条件
冷冻剂 ETHANE
|
分辨率 2.21 Å |
| 7QI5 Human mitochondrial ribosome in complex with mRNA, A/A-, P/P- and E/E-tRNAs at 2.63 A resolution 提交 2021-12-14 | Assembly 1 蛋白–RNA 异源复合物；蛋白 × 87 PDB 声明：94-meric（94） 与全部聚合物一致 |
链 AS
1–190（190 aa）
|
未记录 | NAD NICOTINAMIDE-ADENINE-DINUCLEOTIDE × 1 SPM SPERMINE × 1 SPD SPERMIDINE × 4 MG MAGNESIUM ION × 208 K POTASSIUM ION × 54 ZN ZINC ION × 3 FES FE2/S2 (INORGANIC) CLUSTER × 3 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 PUT 1,4-DIAMINOBUTANE × 1 VAL VALINE × 1 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 7.5
cryo-EM玻璃化条件
冷冻剂 ETHANE
|
分辨率 2.63 Å |
| 7QI6 Human mitochondrial ribosome in complex with mRNA, A/P- and P/E-tRNAs at 2.98 A resolution 提交 2021-12-14 | Assembly 1 蛋白–RNA 异源复合物；蛋白 × 87 PDB 声明：93-meric（93） 与全部聚合物一致 |
链 AS
1–190（190 aa）
|
未记录 | NAD NICOTINAMIDE-ADENINE-DINUCLEOTIDE × 1 SPM SPERMINE × 1 SPD SPERMIDINE × 4 K POTASSIUM ION × 54 MG MAGNESIUM ION × 202 ZN ZINC ION × 3 FES FE2/S2 (INORGANIC) CLUSTER × 3 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 PUT 1,4-DIAMINOBUTANE × 1 VAL VALINE × 1 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 7.5
cryo-EM玻璃化条件
冷冻剂 ETHANE
|
分辨率 2.98 Å |
| 8ANY Human mitochondrial ribosome in complex with LRPPRC, SLIRP, A-site, P-site, E-site tRNAs and mRNA 提交 2022-08-06 | Assembly 1 蛋白–RNA 异源复合物；蛋白 × 89 PDB 声明：96-meric（96） 与全部聚合物一致 |
链 AS
1–190（190 aa）
|
未记录 | NAD NICOTINAMIDE-ADENINE-DINUCLEOTIDE × 1 SPM SPERMINE × 1 SPD SPERMIDINE × 4 MG MAGNESIUM ION × 206 K POTASSIUM ION × 49 ZN ZINC ION × 3 FES FE2/S2 (INORGANIC) CLUSTER × 3 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 PUT 1,4-DIAMINOBUTANE × 1 VAL VALINE × 1 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 7.5
cryo-EM玻璃化条件
冷冻剂 ETHANE
|
分辨率 2.85 Å |
| 8CSP Human mitochondrial small subunit assembly intermediate (State A) 提交 2022-05-13 | Assembly 1 蛋白–RNA 异源复合物；蛋白 × 32 PDB 声明：33-meric（33） 与全部聚合物一致 |
链 S
1–190（190 aa）
|
未记录 | SF4 IRON/SULFUR CLUSTER × 1 SAM S-ADENOSYLMETHIONINE × 1 K POTASSIUM ION × 4 MG MAGNESIUM ION × 28 FES FE2/S2 (INORGANIC) CLUSTER × 2 ZN ZINC ION × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 7.5
cryo-EM玻璃化条件
冷冻剂 ETHANE
|
分辨率 2.66 Å |
| 8CSQ Human mitochondrial small subunit assembly intermediate (State B) 提交 2022-05-13 | Assembly 1 蛋白–RNA 异源复合物；蛋白 × 32 PDB 声明：33-meric（33） 与全部聚合物一致 |
链 S
1–190（190 aa）
|
未记录 | SF4 IRON/SULFUR CLUSTER × 1 SAM S-ADENOSYLMETHIONINE × 1 K POTASSIUM ION × 4 MG MAGNESIUM ION × 27 ZN ZINC ION × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 7.5
cryo-EM玻璃化条件
冷冻剂 ETHANE
|
分辨率 2.54 Å |
| 8CSR Human mitochondrial small subunit assembly intermediate (State C) 提交 2022-05-13 | Assembly 1 蛋白–RNA 异源复合物；蛋白 × 33 PDB 声明：34-meric（34） 与全部聚合物一致 |
链 S
1–190（190 aa）
|
未记录 | SF4 IRON/SULFUR CLUSTER × 1 SAM S-ADENOSYLMETHIONINE × 1 K POTASSIUM ION × 5 MG MAGNESIUM ION × 31 NAD NICOTINAMIDE-ADENINE-DINUCLEOTIDE × 1 ZN ZINC ION × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 7.5
cryo-EM玻璃化条件
冷冻剂 ETHANE
|
分辨率 2.54 Å |
| 8CSS Human mitochondrial small subunit assembly intermediate (State D) 提交 2022-05-13 | Assembly 1 蛋白–RNA 异源复合物；蛋白 × 32 PDB 声明：33-meric（33） 与全部聚合物一致 |
链 S
1–190（190 aa）
|
未记录 | SF4 IRON/SULFUR CLUSTER × 1 SAM S-ADENOSYLMETHIONINE × 1 K POTASSIUM ION × 7 MG MAGNESIUM ION × 32 NAD NICOTINAMIDE-ADENINE-DINUCLEOTIDE × 1 ZN ZINC ION × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 7.5
cryo-EM玻璃化条件
冷冻剂 ETHANE
|
分辨率 2.36 Å |
| 8CST Human mitochondrial small subunit assembly intermediate (State E) 提交 2022-05-13 | Assembly 1 蛋白–RNA 异源复合物；蛋白 × 31 PDB 声明：32-meric（32） 与全部聚合物一致 |
链 S
1–190（190 aa）
|
未记录 | SF4 IRON/SULFUR CLUSTER × 1 SAM S-ADENOSYLMETHIONINE × 1 K POTASSIUM ION × 4 MG MAGNESIUM ION × 30 NAD NICOTINAMIDE-ADENINE-DINUCLEOTIDE × 1 ZN ZINC ION × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 7.5
cryo-EM玻璃化条件
冷冻剂 ETHANE
|
分辨率 2.85 Å |
| 8CSU Human mitochondrial small subunit assembly intermediate (State C*) 提交 2022-05-13 | Assembly 1 蛋白–RNA 异源复合物；蛋白 × 32 PDB 声明：33-meric（33） 与全部聚合物一致 |
链 S
1–190（190 aa）
|
未记录 | SF4 IRON/SULFUR CLUSTER × 1 SAM S-ADENOSYLMETHIONINE × 1 K POTASSIUM ION × 5 MG MAGNESIUM ION × 26 NAD NICOTINAMIDE-ADENINE-DINUCLEOTIDE × 1 ZN ZINC ION × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 7.5
cryo-EM玻璃化条件
冷冻剂 ETHANE
|
分辨率 3.03 Å |
| 8K2A Cryo-EM structure of the human 55S mitoribosome with Tigecycline 提交 2023-07-12 | Assembly 1 蛋白–RNA 异源复合物；蛋白 × 80 PDB 声明：83-meric（83） 与全部聚合物一致 |
链 SY
1–190（190 aa）
|
未记录 | MG MAGNESIUM ION × 145 T1C TIGECYCLINE × 4 ZN ZINC ION × 7 GDP GUANOSINE-5'-DIPHOSPHATE × 1 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 7.4
cryo-EM玻璃化条件
冷冻剂 ETHANE
|
分辨率 2.90 Å |
| 8OIR 55S human mitochondrial ribosome with mtRF1 and P-site tRNA 提交 2023-03-23 | Assembly 1 蛋白–RNA 异源复合物；蛋白 × 88 PDB 声明：94-meric（94） 与全部聚合物一致 |
链 AS
1–190（190 aa）
|
未记录 | K POTASSIUM ION × 51 MG MAGNESIUM ION × 354 VAL VALINE × 1 ZN ZINC ION × 3 FS2 FE-S-O HYBRID CLUSTER × 3 MET METHIONINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 7.5
cryo-EM玻璃化条件
冷冻剂 ETHANE-PROPANE
|
分辨率 3.10 Å |
| 8OIS 28S human mitochondrial small ribosomal subunit with mtRF1 and P-site tRNA 提交 2023-03-23 | Assembly 1 蛋白–RNA 异源复合物；蛋白 × 33 PDB 声明：36-meric（36） 与全部聚合物一致 |
链 AS
1–190（190 aa）
|
未记录 | K POTASSIUM ION × 17 MG MAGNESIUM ION × 126 MET METHIONINE × 1 ZN ZINC ION × 1 FS2 FE-S-O HYBRID CLUSTER × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 7.5
cryo-EM玻璃化条件
冷冻剂 ETHANE-PROPANE
|
分辨率 3.00 Å |
| 8QRK mt-SSU assembly intermediate in GTPBP8 knock-out cells, state 1 提交 2023-10-09 | Assembly 1 蛋白–RNA 异源复合物；蛋白 × 29 PDB 声明：30-meric（30） 与全部聚合物一致 |
链 S
1–190（190 aa）
|
未记录 | MG MAGNESIUM ION × 49 NAD NICOTINAMIDE-ADENINE-DINUCLEOTIDE × 1 SPM SPERMINE × 1 SRY STREPTOMYCIN × 1 K POTASSIUM ION × 17 ZN ZINC ION × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 7.5
cryo-EM玻璃化条件
冷冻剂 ETHANE
|
分辨率 6.69 Å |
| 8QRL mt-SSU assembly intermediate in GTPBP8 knock-out cells, state 2 提交 2023-10-09 | Assembly 1 蛋白–RNA 异源复合物；蛋白 × 30 PDB 声明：31-meric（31） 与全部聚合物一致 |
链 S
1–190（190 aa）
|
未记录 | NAD NICOTINAMIDE-ADENINE-DINUCLEOTIDE × 1 MG MAGNESIUM ION × 64 K POTASSIUM ION × 16 ZN ZINC ION × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 SAH S-ADENOSYL-L-HOMOCYSTEINE × 1 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 7.5
cryo-EM玻璃化条件
冷冻剂 ETHANE
|
分辨率 3.34 Å |
| 8QRM mt-SSU assembly intermediate in GTPBP8 knock-out cells, state 3 提交 2023-10-09 | Assembly 1 蛋白–RNA 异源复合物；蛋白 × 31 PDB 声明：32-meric（32） 与全部聚合物一致 |
链 S
1–190（190 aa）
|
未记录 | NAD NICOTINAMIDE-ADENINE-DINUCLEOTIDE × 1 SPM SPERMINE × 1 SRY STREPTOMYCIN × 1 MG MAGNESIUM ION × 59 K POTASSIUM ION × 17 ZN ZINC ION × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 7.5
cryo-EM玻璃化条件
冷冻剂 ETHANE
|
分辨率 3.05 Å |
| 8QRN mt-SSU in GTPBP8 knock-out cells, state 4 提交 2023-10-09 | Assembly 1 蛋白–RNA 异源复合物；蛋白 × 31 PDB 声明：34-meric（34） 与全部聚合物一致 |
链 S
1–190（190 aa）
|
未记录 | NAD NICOTINAMIDE-ADENINE-DINUCLEOTIDE × 1 SPM SPERMINE × 1 SRY STREPTOMYCIN × 1 MG MAGNESIUM ION × 58 K POTASSIUM ION × 18 ZN ZINC ION × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 FME N-FORMYLMETHIONINE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 7.5
cryo-EM玻璃化条件
冷冻剂 ETHANE
|
分辨率 2.98 Å |
| 8RRI Human mitochondrial ribosome in complex with antibiotic tigecycline 提交 2024-01-22 | Assembly 1 蛋白–RNA 异源复合物；蛋白 × 80 PDB 声明：86-meric（86） 与全部聚合物一致 |
链 AS
1–190（190 aa）
|
未记录 | VAL VALINE × 1 MG MAGNESIUM ION × 94 FES FE2/S2 (INORGANIC) CLUSTER × 3 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 T1C TIGECYCLINE × 3 K POTASSIUM ION × 16 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 7.5
cryo-EM玻璃化条件
冷冻剂 ETHANE
|
分辨率 2.40 Å |
| 8XT0 Cryo-EM structure of the human 55S mitoribosome with 5um Tigecycline 提交 2024-01-10 | Assembly 1 蛋白–RNA 异源复合物；蛋白 × 80 PDB 声明：83-meric（83） 与全部聚合物一致 |
链 SY
1–190（190 aa）
|
未记录 | MG MAGNESIUM ION × 145 T1C TIGECYCLINE × 3 ZN ZINC ION × 7 GDP GUANOSINE-5'-DIPHOSPHATE × 1 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 7.4
cryo-EM玻璃化条件
冷冻剂 ETHANE
|
分辨率 3.20 Å |
| 8XT2 Cryo-EM structure of the human 55S mitoribosome with 10uM Tigecycline 提交 2024-01-10 | Assembly 1 蛋白–RNA 异源复合物；蛋白 × 80 PDB 声明：83-meric（83） 与全部聚合物一致 |
链 SY
1–190（190 aa）
|
未记录 | MG MAGNESIUM ION × 145 T1C TIGECYCLINE × 3 ZN ZINC ION × 7 GDP GUANOSINE-5'-DIPHOSPHATE × 1 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 7.4
cryo-EM玻璃化条件
冷冻剂 ETHANE
|
分辨率 3.30 Å |
| 9G5B Assembly intermediate of human mitochondrial ribosome small subunit (State A) 提交 2024-07-16 | Assembly 1 蛋白–RNA 异源复合物；蛋白 × 31 PDB 声明：32-meric（32） 与全部聚合物一致 |
链 S
1–190（190 aa）
|
未记录 | MG MAGNESIUM ION × 12 ZN ZINC ION × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 7.4；20mM HEPES-HCl, 100mM KCl, 20mM MgCl2, 0.02% DDM, 1mM PMSF, 1x protease-inhibitor mix, 0.5mM GMP-PNP
cryo-EM玻璃化条件
冷冻剂 ETHANE
|
分辨率 3.20 Å |
| 9G5C Assembly intermediate of human mitochondrial ribosome small subunit (State B) 提交 2024-07-16 | Assembly 1 蛋白–RNA 异源复合物；蛋白 × 31 PDB 声明：32-meric（32） 与全部聚合物一致 |
链 S
1–190（190 aa）
|
未记录 | MG MAGNESIUM ION × 23 K POTASSIUM ION × 4 ZN ZINC ION × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 SAH S-ADENOSYL-L-HOMOCYSTEINE × 1 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 7.4；20mM HEPES-HCl, 100mM KCl, 20mM MgCl2, 0.02% DDM, 1mM PMSF, 1x protease-inhibitor mix, 0.5mM GMP-PNP
cryo-EM玻璃化条件
冷冻剂 ETHANE
|
分辨率 3.00 Å |
| 9G5D Assembly intermediate of human mitochondrial ribosome small subunit (State C) 提交 2024-07-16 | Assembly 1 蛋白–RNA 异源复合物；蛋白 × 31 PDB 声明：32-meric（32） 与全部聚合物一致 |
链 S
1–190（190 aa）
|
未记录 | MG MAGNESIUM ION × 10 K POTASSIUM ION × 1 NAD NICOTINAMIDE-ADENINE-DINUCLEOTIDE × 1 ZN ZINC ION × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 SAH S-ADENOSYL-L-HOMOCYSTEINE × 1 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 7.4；20mM HEPES-HCl, 100mM KCl, 20mM MgCl2, 0.02% DDM, 1mM PMSF, 1x protease-inhibitor mix, 0.5mM GMP-PNP
cryo-EM玻璃化条件
冷冻剂 ETHANE
|
分辨率 3.00 Å |
| 9G5E Translation-initiation state of human mitochondrial ribosome small subunit (State F) 提交 2024-07-16 | Assembly 1 蛋白–RNA 异源复合物；蛋白 × 31 PDB 声明：32-meric（32） 与全部聚合物一致 |
链 S
1–190（190 aa）
|
未记录 | MG MAGNESIUM ION × 45 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 K POTASSIUM ION × 1 ZN ZINC ION × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 7.4；20mM HEPES-HCl, 100mM KCl, 20mM MgCl2, 0.02% DDM, 1mM PMSF, 1x protease-inhibitor mix, 0.5mM GMP-PNP
cryo-EM玻璃化条件
冷冻剂 ETHANE
|
分辨率 3.20 Å |
| 9H51 Assembly intermediate of human mitochondrial ribosome small subunit in complex with PUS1, METTL15 and RBFA(IN conformation) (state M1) 提交 2024-10-22 | Assembly 1 蛋白–RNA 异源复合物；蛋白 × 33 PDB 声明：34-meric（34） 与全部聚合物一致 |
链 S
1–190（190 aa）
|
未记录 | NAD NICOTINAMIDE-ADENINE-DINUCLEOTIDE × 1 SPM SPERMINE × 1 MG MAGNESIUM ION × 40 K POTASSIUM ION × 6 ZN ZINC ION × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 SAH S-ADENOSYL-L-HOMOCYSTEINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 7.5
cryo-EM玻璃化条件
冷冻剂 ETHANE
|
分辨率 3.10 Å |
| 9H54 Assembly intermediate of human mitochondrial ribosome small subunit in complex with NOA1 and partial RBFA (state N2) 提交 2024-10-22 | Assembly 1 蛋白–RNA 异源复合物；蛋白 × 30 PDB 声明：31-meric（31） 与全部聚合物一致 |
链 S
1–190（190 aa）
|
未记录 | MG MAGNESIUM ION × 42 K POTASSIUM ION × 11 ZN ZINC ION × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 2 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 7.5
cryo-EM玻璃化条件
冷冻剂 ETHANE
|
分辨率 3.00 Å |
| 9H55 Assembly intermediate of human mitochondrial ribosome small subunit in complex with NOA1 and TFB1M (state N3) 提交 2024-10-22 | Assembly 1 蛋白–RNA 异源复合物；蛋白 × 31 PDB 声明：32-meric（32） 与全部聚合物一致 |
链 S
1–190（190 aa）
|
未记录 | MG MAGNESIUM ION × 41 K POTASSIUM ION × 10 ZN ZINC ION × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 2 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 7.5
cryo-EM玻璃化条件
冷冻剂 ETHANE
|
分辨率 3.40 Å |
| 9HFM Translation-initiation state of human mitochondrial ribosome small subunit (State D) 提交 2024-11-18 | Assembly 1 蛋白–RNA 异源复合物；蛋白 × 31 PDB 声明：32-meric（32） 与全部聚合物一致 |
链 S
1–190（190 aa）
|
未记录 | MG MAGNESIUM ION × 44 K POTASSIUM ION × 1 ZN ZINC ION × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 7.4；20mM HEPES-HCl, 100mM KCl, 20mM MgCl2, 0.02% DDM, 1mM PMSF, 1x protease-inhibitor mix, 0.5mM GMP-PNP
cryo-EM玻璃化条件
冷冻剂 ETHANE
|
分辨率 3.00 Å |
| 9HFN Translation-initiation state of human mitochondrial ribosome small subunit (State E) 提交 2024-11-18 | Assembly 1 蛋白–RNA 异源复合物；蛋白 × 32 PDB 声明：33-meric（33） 与全部聚合物一致 |
链 S
1–190（190 aa）
|
未记录 | MG MAGNESIUM ION × 45 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 K POTASSIUM ION × 1 ZN ZINC ION × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 7.4；20mM HEPES-HCl, 100mM KCl, 20mM MgCl2, 0.02% DDM, 1mM PMSF, 1x protease-inhibitor mix, 0.5mM GMP-PNP
cryo-EM玻璃化条件
冷冻剂 ETHANE
|
分辨率 3.30 Å |
| 9HFO Translation-initiation state of human mitochondrial ribosome small subunit (State G) 提交 2024-11-18 | Assembly 1 蛋白–RNA 异源复合物；蛋白 × 31 PDB 声明：34-meric（34） 与全部聚合物一致 |
链 S
1–190（190 aa）
|
未记录 | MG MAGNESIUM ION × 45 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 K POTASSIUM ION × 1 ZN ZINC ION × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 7.4；20mM HEPES-HCl, 100mM KCl, 20mM MgCl2, 0.02% DDM, 1mM PMSF, 1x protease-inhibitor mix, 0.5mM GMP-PNP
cryo-EM玻璃化条件
冷冻剂 ETHANE
|
分辨率 3.10 Å |
| 9IGT Assembly intermediate of human mitochondrial ribosome small subunit bound to METTL15, RBFA, and mtIF2 (State M2.1) 提交 2025-02-20 | Assembly 1 蛋白–RNA 异源复合物；蛋白 × 33 PDB 声明：34-meric（34） 与全部聚合物一致 |
链 S
1–190（190 aa）
|
未记录 | MG MAGNESIUM ION × 12 ZN ZINC ION × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 2 NAD NICOTINAMIDE-ADENINE-DINUCLEOTIDE × 1 SPM SPERMINE × 1 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 7.5
cryo-EM玻璃化条件
冷冻剂 ETHANE
|
分辨率 5.96 Å |
| 9IGU Assembly intermediate of human mitochondrial ribosome small subunit bound to METTL15 and RBFA (Inward conformation) (State M2) 提交 2025-02-20 | Assembly 1 蛋白–RNA 异源复合物；蛋白 × 31 PDB 声明：32-meric（32） 与全部聚合物一致 |
链 S
1–190（190 aa）
|
未记录 | MG MAGNESIUM ION × 31 FES FE2/S2 (INORGANIC) CLUSTER × 2 ZN ZINC ION × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 NAD NICOTINAMIDE-ADENINE-DINUCLEOTIDE × 1 SPM SPERMINE × 1 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 7.5
cryo-EM玻璃化条件
冷冻剂 ETHANE
|
分辨率 3.64 Å |
| 9OLF Membrane-associated human mitoribosome in complex with TACO1 提交 2025-05-12 | Assembly 1 蛋白–RNA 异源复合物；蛋白 × 93 PDB 声明：100-meric（100） 与全部聚合物一致 |
链 AS
1–190（190 aa）
|
未记录 | ZN ZINC ION × 3 SPD SPERMIDINE × 7 MG MAGNESIUM ION × 205 PUT 1,4-DIAMINOBUTANE × 1 K POTASSIUM ION × 54 SPM SPERMINE × 2 NAD NICOTINAMIDE-ADENINE-DINUCLEOTIDE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 3 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 VAL VALINE × 1 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 7.4
cryo-EM玻璃化条件
冷冻剂 ETHANE
|
分辨率 2.46 Å |
| 9PG8 In situ structure of the human mitoribosome in the P-E state 提交 2025-07-07 | Assembly 1 蛋白–RNA 异源复合物；蛋白 × 86 PDB 声明：93-meric（93） 与全部聚合物一致 |
链 AS
1–190（190 aa）
|
未记录 | ZN ZINC ION × 3 K POTASSIUM ION × 54 SPD SPERMIDINE × 8 PUT 1,4-DIAMINOBUTANE × 1 MG MAGNESIUM ION × 205 VAL VALINE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 3 NAD NICOTINAMIDE-ADENINE-DINUCLEOTIDE × 1 SPM SPERMINE × 3 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 7.4
cryo-EM玻璃化条件
冷冻剂 ETHANE
|
分辨率 3.06 Å |
| 9PGF In situ structure of the human mitoribosome in the P state 提交 2025-07-07 | Assembly 1 蛋白–RNA 异源复合物；蛋白 × 86 PDB 声明：91-meric（91） 与全部聚合物一致 |
链 AS
1–190（190 aa）
|
未记录 | ZN ZINC ION × 3 K POTASSIUM ION × 54 SPD SPERMIDINE × 8 PUT 1,4-DIAMINOBUTANE × 1 MG MAGNESIUM ION × 205 VAL VALINE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 3 NAD NICOTINAMIDE-ADENINE-DINUCLEOTIDE × 1 SPM SPERMINE × 3 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 7.4
cryo-EM玻璃化条件
冷冻剂 ETHANE
|
分辨率 2.93 Å |
| 9PGI In situ structure of the human mitoribosome in the A-P-E state with TACO1 提交 2025-07-07 | Assembly 1 蛋白–RNA 异源复合物；蛋白 × 87 PDB 声明：94-meric（94） 与全部聚合物一致 |
链 AS
1–190（190 aa）
|
未记录 | ZN ZINC ION × 3 K POTASSIUM ION × 54 SPD SPERMIDINE × 7 PUT 1,4-DIAMINOBUTANE × 1 MG MAGNESIUM ION × 204 FES FE2/S2 (INORGANIC) CLUSTER × 3 NAD NICOTINAMIDE-ADENINE-DINUCLEOTIDE × 1 SPM SPERMINE × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 VAL VALINE × 1 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 7.4
cryo-EM玻璃化条件
冷冻剂 ETHANE
|
分辨率 3.02 Å |
| 9PGL In situ structure of the human mitoribosome in the A-P state with TACO1 提交 2025-07-08 | Assembly 1 蛋白–RNA 异源复合物；蛋白 × 86 PDB 声明：92-meric（92） 与全部聚合物一致 |
链 AS
1–190（190 aa）
|
未记录 | ZN ZINC ION × 3 K POTASSIUM ION × 54 SPD SPERMIDINE × 8 PUT 1,4-DIAMINOBUTANE × 1 MG MAGNESIUM ION × 204 FES FE2/S2 (INORGANIC) CLUSTER × 3 NAD NICOTINAMIDE-ADENINE-DINUCLEOTIDE × 1 SPM SPERMINE × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 VAL VALINE × 1 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 7.4
cryo-EM玻璃化条件
冷冻剂 ETHANE
|
分辨率 2.90 Å |
| 9PGM In situ structure of the human mitoribosome in the P-E state from TACO1-knockout cells 提交 2025-07-08 | Assembly 1 蛋白–RNA 异源复合物；蛋白 × 85 PDB 声明：91-meric（91） 与全部聚合物一致 |
链 AS
1–190（190 aa）
|
未记录 | ZN ZINC ION × 3 K POTASSIUM ION × 54 SPD SPERMIDINE × 7 PUT 1,4-DIAMINOBUTANE × 1 MG MAGNESIUM ION × 204 FES FE2/S2 (INORGANIC) CLUSTER × 3 NAD NICOTINAMIDE-ADENINE-DINUCLEOTIDE × 1 SPM SPERMINE × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 VAL VALINE × 1 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 7.4
cryo-EM玻璃化条件
冷冻剂 ETHANE
|
分辨率 3.18 Å |
| 9PRD In situ structure of the human mitoribosome in the P state from TACO1-knockout cells 提交 2025-07-23 | Assembly 1 蛋白–RNA 异源复合物；蛋白 × 84 PDB 声明：89-meric（89） 与全部聚合物一致 |
链 AS
1–190（190 aa）
|
未记录 | ZN ZINC ION × 3 K POTASSIUM ION × 54 SPD SPERMIDINE × 7 PUT 1,4-DIAMINOBUTANE × 1 MG MAGNESIUM ION × 204 FES FE2/S2 (INORGANIC) CLUSTER × 3 NAD NICOTINAMIDE-ADENINE-DINUCLEOTIDE × 1 SPM SPERMINE × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 VAL VALINE × 1 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 7.4
cryo-EM玻璃化条件
冷冻剂 ETHANE
|
分辨率 3.31 Å |
| 9PRQ In situ structure of human mitoribosome in the A/T-P state from TACO1-knockout cells 提交 2025-07-24 | Assembly 1 蛋白–RNA 异源复合物；蛋白 × 85 PDB 声明：91-meric（91） 与全部聚合物一致 |
链 AS
1–190（190 aa）
|
未记录 | ZN ZINC ION × 3 K POTASSIUM ION × 54 SPD SPERMIDINE × 7 PUT 1,4-DIAMINOBUTANE × 1 MG MAGNESIUM ION × 204 FES FE2/S2 (INORGANIC) CLUSTER × 3 NAD NICOTINAMIDE-ADENINE-DINUCLEOTIDE × 1 SPM SPERMINE × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 VAL VALINE × 1 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 7.4
cryo-EM玻璃化条件
冷冻剂 ETHANE
|
分辨率 3.34 Å |
| 9PRX In situ structure of the human mitoribosome in the A-P state from TACO1-knockout cells 提交 2025-07-24 | Assembly 1 蛋白–RNA 异源复合物；蛋白 × 84 PDB 声明：90-meric（90） 与全部聚合物一致 |
链 AS
1–190（190 aa）
|
未记录 | VAL VALINE × 1 ZN ZINC ION × 3 K POTASSIUM ION × 54 SPD SPERMIDINE × 7 PUT 1,4-DIAMINOBUTANE × 1 MG MAGNESIUM ION × 204 FES FE2/S2 (INORGANIC) CLUSTER × 3 NAD NICOTINAMIDE-ADENINE-DINUCLEOTIDE × 1 SPM SPERMINE × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 7.4
cryo-EM玻璃化条件
冷冻剂 ETHANE
|
分辨率 3.23 Å |
| 9PS0 In situ structure of the human mitoribosome in the A/P-P/E state from TACO1-knockout cells 提交 2025-07-24 | Assembly 1 蛋白–RNA 异源复合物；蛋白 × 85 PDB 声明：91-meric（91） 与全部聚合物一致 |
链 AS
1–190（190 aa）
|
未记录 | ZN ZINC ION × 3 K POTASSIUM ION × 52 SPD SPERMIDINE × 7 PUT 1,4-DIAMINOBUTANE × 1 MG MAGNESIUM ION × 204 NAD NICOTINAMIDE-ADENINE-DINUCLEOTIDE × 1 SPM SPERMINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 3 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 VAL VALINE × 1 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 7.4
cryo-EM玻璃化条件
冷冻剂 ETHANE
|
分辨率 3.29 Å |
| 9PS7 In situ structure of the human mitoribosome in the A/T-P-E state from TACO1-knockout cells 提交 2025-07-25 | Assembly 1 蛋白–RNA 异源复合物；蛋白 × 86 PDB 声明：93-meric（93） 与全部聚合物一致 |
链 AS
1–190（190 aa）
|
未记录 | ZN ZINC ION × 3 K POTASSIUM ION × 54 SPD SPERMIDINE × 7 PUT 1,4-DIAMINOBUTANE × 1 MG MAGNESIUM ION × 204 FES FE2/S2 (INORGANIC) CLUSTER × 3 NAD NICOTINAMIDE-ADENINE-DINUCLEOTIDE × 1 SPM SPERMINE × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 VAL VALINE × 1 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 7.4
cryo-EM玻璃化条件
冷冻剂 ETHANE
|
分辨率 3.08 Å |
| 9PSI In situ structure of the human mitoribosome in the A-P-E state from TACO1-knockout cells 提交 2025-07-25 | Assembly 1 蛋白–RNA 异源复合物；蛋白 × 85 PDB 声明：92-meric（92） 与全部聚合物一致 |
链 AS
1–190（190 aa）
|
未记录 | ZN ZINC ION × 3 K POTASSIUM ION × 54 SPD SPERMIDINE × 7 PUT 1,4-DIAMINOBUTANE × 1 MG MAGNESIUM ION × 204 FES FE2/S2 (INORGANIC) CLUSTER × 3 NAD NICOTINAMIDE-ADENINE-DINUCLEOTIDE × 1 SPM SPERMINE × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 VAL VALINE × 1 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 7.4
cryo-EM玻璃化条件
冷冻剂 ETHANE
|
分辨率 3.12 Å |
| 9PSM In situ structure of the human mitoribosome in the A/P-P/E state 提交 2025-07-25 | Assembly 1 蛋白–RNA 异源复合物；蛋白 × 87 PDB 声明：93-meric（93） 与全部聚合物一致 |
链 AS
1–190（190 aa）
|
未记录 | ZN ZINC ION × 3 K POTASSIUM ION × 54 SPD SPERMIDINE × 8 PUT 1,4-DIAMINOBUTANE × 1 MG MAGNESIUM ION × 204 FES FE2/S2 (INORGANIC) CLUSTER × 3 NAD NICOTINAMIDE-ADENINE-DINUCLEOTIDE × 1 SPM SPERMINE × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 VAL VALINE × 1 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 7.4
cryo-EM玻璃化条件
冷冻剂 ETHANE
|
分辨率 2.98 Å |
| 9UWH human mitoribosome trapped by retapamulin 提交 2025-05-12 | Assembly 1 蛋白–RNA 异源复合物；蛋白 × 81 PDB 声明：86-meric（86） 与全部聚合物一致 |
链 AS
1–190（190 aa）
|
未记录 | MG MAGNESIUM ION × 208 K POTASSIUM ION × 53 G34 Retapamulin × 1 VAL VALINE × 1 NAD NICOTINAMIDE-ADENINE-DINUCLEOTIDE × 1 SPD SPERMIDINE × 1 ZN ZINC ION × 3 FES FE2/S2 (INORGANIC) CLUSTER × 3 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 7.5
cryo-EM玻璃化条件
冷冻剂 ETHANE
|
分辨率 3.00 Å |