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1a73

INTRON-ENCODED ENDONUCLEASE I-PPOI COMPLEXED WITH DNA

Method: X-RAY DIFFRACTION D_max: 78.8 Å Quality: GOOD

SAXS Profile

P(r) Distribution

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1. Structure Basics

entry_id	1a73
deposition_date	1998-03-19
title	INTRON-ENCODED ENDONUCLEASE I-PPOI COMPLEXED WITH DNA
keywords	COMPLEX (HOMING ENDONUCLEASE-DNA), INTRON, ZINC, DNA BINDING, PROTEIN FOLDING, HYDROLASE-DNA COMPLEX; HYDROLASE/DNA
method	X-RAY DIFFRACTION

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Download Data

SAXS curve (.dat) P(r) distribution (.pr.out) Structure (CIF)

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2. SAXS Parameters (CRYSOL)

rg_guinier	24.74 Å
rg_electron	24.51 Å
i0	55543200.00
molecular_weight	48543.0 kDa
excluded_volume	56469 Å³
envelope_volume	70808 Å³
shell_volume	24630 Å³
envelope_diameter	80.7 Å
shell_rg	31.20 Å
envelope_rg	24.57 Å
shape_rg	24.52 Å
total_rg	25.11 Å
total_atoms	3352
n_residues	366
n_harmonics	20
q_range	— – 0.5000 Å⁻¹
n_points	101
shell_type	directional
solvent_density	0.3340 e/Å³
contrast_shell	0.0300 e/Å³
crysol_version	4.1.3

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3. P(r) Analysis (GNOM)

dmax	78.8 Å
rg_real	24.78 Å
rg_real_error	0.40 Å
i0_real	5.5540e+07
i0_real_error	6.6220e+05
rg_reciprocal	24.77 Å
i0_reciprocal	55540000.0000
total_estimate	0.9000
solution_quality	GOOD a GOOD solution
n_peaks	2
r_peak_primary	26.3 Å
skewness	0.350
kurtosis	-0.486
angular_range	— – 0.3200 Å⁻¹
current_alpha	0.0000
highest_alpha	5884000.0000
n_real_points	64
gnom_version	4.1.3
quality_criteria	AN1: 0.000; Oscil: 0.924; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.957; Smooth: 0.966

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4. Crystallography & Experiment

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5. Entities & Polymer Info (6)

Entity 1 polymer

description	;DNA (5'-D(TPTPGPAPCPTPCPTPCPTPTPAP*A)-3') ;
formula_weight	3916.6 kDa
src_method	syn
entity_count	2

Polymer

type	polydeoxyribonucleotide
strand_id	C,E
atom_count	3743

TTGACTCTCTTAA

Entity 2 polymer

description	;DNA (5'-D(PGPAPGPAPGPTPCPA)-3') ;
formula_weight	2475.7 kDa
src_method	syn
entity_count	2

Polymer

type	polydeoxyribonucleotide
strand_id	D,F

GAGAGTCA

Entity 3 polymer

description	INTRON 3 (I-PPO) ENCODED ENDONUCLEASE
formula_weight	17811.2 kDa
src_method	man
entity_count	2

Polymer

type	polypeptide(L)
strand_id	A,B

MALTNAQILAVIDSWEETVGQFPVITHHVPLGGGLQGTLHCYEIPLAAPYGVGFAKNGPTRWQYKRTINQVVHRWGSHTVPFLLEPDNINGKTCTASHLCHNTRCHNPLHLCWESLDDNKGRNWCPGPNGGCVHAVVCLRQGPLYGPGATVAGPQQRGSHFVV

Source

organism	Physarum polycephalum
ncbi_taxonomy_id	5791
host_organism	Escherichia coli BL21(DE3)

Entity 4 non-polymer

description	MAGNESIUM ION
formula_weight	24.3 kDa
src_method	syn
entity_count	2

Entity 5 non-polymer

description	ZINC ION
formula_weight	65.4 kDa
src_method	syn
entity_count	4

Entity 6 water

description	water
formula_weight	18.0 kDa
src_method	nat
entity_count	391

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6. Citations (2)

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length_a	113.700 Å
length_b	113.700 Å
length_c	88.400 Å
angle_alpha	90.00 °
angle_beta	90.00 °
angle_gamma	120.00 °
z_pdb	12

space_group	P 31 2 1
int_tables_number	152
crystal_system	—

resolution	1.800 Å
resolution_low	50.000 Å
r_factor	0.2080
r_work	0.2080
r_free	0.2970

fit	/app/data/saxs_computation/a7/1a73/1a73.fit
pddf	/app/data/pr_computation/a7/1a73/1a73.pr.out
profile	/app/data/saxs_computation/a7/1a73/1a73.dat

INTRON-ENCODED ENDONUCLEASE I-PPOI COMPLEXED WITH DNA

SAXS Profile

P(r) Distribution

1. Structure Basics

Download Data

2. SAXS Parameters (CRYSOL)

3. P(r) Analysis (GNOM)

4. Crystallography & Experiment

Unit Cell

Symmetry

Refinement

Diffraction

5. Entities & Polymer Info (6)

Entity 1 polymer

Polymer

Entity 2 polymer

Polymer

Entity 3 polymer

Polymer

Source

Entity 4 non-polymer

Entity 5 non-polymer

Entity 6 water

6. Citations (2)

7. Files & Curves (10)

Files (7)

Curves (3)