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1evx

APO CRYSTAL STRUCTURE OF THE HOMING ENDONUCLEASE, I-PPOI

Method: X-RAY DIFFRACTION D_max: 81.1 Å Quality: GOOD

SAXS Profile

P(r) Distribution

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1. Structure Basics

entry_id	1evx
deposition_date	2000-04-20
title	APO CRYSTAL STRUCTURE OF THE HOMING ENDONUCLEASE, I-PPOI
keywords	DNA binding B-sheets; C-terminal exchanged dimer interface, HYDROLASE; HYDROLASE
method	X-RAY DIFFRACTION

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Download Data

SAXS curve (.dat) P(r) distribution (.pr.out) Structure (CIF)

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2. SAXS Parameters (CRYSOL)

rg_guinier	24.54 Å
rg_electron	24.51 Å
i0	24521400.00
molecular_weight	35966.0 kDa
excluded_volume	44076 Å³
envelope_volume	55214 Å³
shell_volume	19827 Å³
envelope_diameter	83.3 Å
shell_rg	30.41 Å
envelope_rg	24.48 Å
shape_rg	24.56 Å
total_rg	25.06 Å
total_atoms	2514
n_residues	324
n_harmonics	20
q_range	— – 0.5000 Å⁻¹
n_points	101
shell_type	directional
solvent_density	0.3340 e/Å³
contrast_shell	0.0300 e/Å³
crysol_version	4.1.3

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3. P(r) Analysis (GNOM)

dmax	81.1 Å
rg_real	24.72 Å
rg_real_error	0.59 Å
i0_real	2.4520e+07
i0_real_error	3.5820e+05
rg_reciprocal	24.68 Å
i0_reciprocal	24520000.0000
total_estimate	0.8647
solution_quality	GOOD a GOOD solution
n_peaks	2
r_peak_primary	23.2 Å
skewness	0.425
kurtosis	-0.531
angular_range	— – 0.3250 Å⁻¹
current_alpha	0.0001
highest_alpha	2971000.0000
n_real_points	64
gnom_version	4.1.3
quality_criteria	AN1: 0.000; Oscil: 0.839; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.740; Smooth: 0.978

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4. Crystallography & Experiment

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5. Entities & Polymer Info (4)

Entity 1 polymer

description	INTRON-ENCODED HOMING ENDONUCLEASE I-PPOI
formula_weight	17680.0 kDa
src_method	man
entity_count	2

Polymer

type	polypeptide(L)
strand_id	A,B
atom_count	2824

ALTNAQILAVIDSWEETVGQFPVITHHVPLGGGLQGTLHCYEIPLAAPYGVGFAKNGPTRWQYKRTINQVVHRWGSHTVPFLLEPDNINGKTCTASHLCHNTRCHNPLHLCWESLDDNKGRNWCPGPNGGCVHAVVCLRQGPLYGPGATVAGPQQRGSHFVV

Source

organism	Physarum polycephalum
ncbi_taxonomy_id	5791
host_organism	Escherichia coli

Entity 2 non-polymer

description	ZINC ION
formula_weight	65.4 kDa
src_method	syn
entity_count	4

Entity 3 non-polymer

description	SULFATE ION
formula_weight	96.1 kDa
src_method	syn
entity_count	4

Entity 4 water

description	water
formula_weight	18.0 kDa
src_method	nat
entity_count	310

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6. Citations (1)

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length_a	52.800 Å
length_b	52.800 Å
length_c	278.800 Å
angle_alpha	90.00 °
angle_beta	90.00 °
angle_gamma	90.00 °
z_pdb	16

space_group	P 41 21 2
int_tables_number	92
crystal_system	—

resolution	2.000 Å
resolution_low	50.000 Å
r_factor	—
r_work	0.1940
r_free	0.2290

fit	/app/data/saxs_computation/ev/1evx/1evx.fit
pddf	/app/data/pr_computation/ev/1evx/1evx.pr.out
profile	/app/data/saxs_computation/ev/1evx/1evx.dat

APO CRYSTAL STRUCTURE OF THE HOMING ENDONUCLEASE, I-PPOI

SAXS Profile

P(r) Distribution

1. Structure Basics

Download Data

2. SAXS Parameters (CRYSOL)

3. P(r) Analysis (GNOM)

4. Crystallography & Experiment

Unit Cell

Symmetry

Refinement

Diffraction