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1cz0

INTRON ENCODED HOMING ENDONUCLEASE I-PPOI/DNA COMPLEX LACKING CATALYTIC METAL ION

Method: X-RAY DIFFRACTION D_max: 79.4 Å Quality: EXCELLENT

SAXS Profile

P(r) Distribution

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1. Structure Basics

entry_id	1cz0
deposition_date	1999-08-31
title	INTRON ENCODED HOMING ENDONUCLEASE I-PPOI/DNA COMPLEX LACKING CATALYTIC METAL ION
keywords	PROTEIN-DNA COMPLEX, DISTORTED DNA, HIS-CYS BOX ZINC BINDING SITE, BETA SHEET DNA BINDING, HYDROLASE-DNA COMPLEX; HYDROLASE/DNA
method	X-RAY DIFFRACTION

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Download Data

SAXS curve (.dat) P(r) distribution (.pr.out) Structure (CIF)

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2. SAXS Parameters (CRYSOL)

rg_guinier	24.91 Å
rg_electron	24.63 Å
i0	55072400.00
molecular_weight	48506.0 kDa
excluded_volume	56495 Å³
envelope_volume	72042 Å³
shell_volume	24928 Å³
envelope_diameter	80.7 Å
shell_rg	31.56 Å
envelope_rg	24.67 Å
shape_rg	24.64 Å
total_rg	25.26 Å
total_atoms	3350
n_residues	366
n_harmonics	20
q_range	— – 0.5000 Å⁻¹
n_points	101
shell_type	directional
solvent_density	0.3340 e/Å³
contrast_shell	0.0300 e/Å³
crysol_version	4.1.3

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3. P(r) Analysis (GNOM)

dmax	79.4 Å
rg_real	24.94 Å
rg_real_error	0.37 Å
i0_real	5.5070e+07
i0_real_error	7.0070e+05
rg_reciprocal	24.94 Å
i0_reciprocal	55070000.0000
total_estimate	0.9006
solution_quality	EXCELLENT a EXCELLENT solution
n_peaks	2
r_peak_primary	26.5 Å
skewness	0.344
kurtosis	-0.493
angular_range	— – 0.3200 Å⁻¹
current_alpha	0.0000
highest_alpha	6178000.0000
n_real_points	64
gnom_version	4.1.3
quality_criteria	AN1: 0.000; Oscil: 0.925; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.959; Smooth: 0.969

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4. Crystallography & Experiment

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5. Entities & Polymer Info (5)

Entity 1 polymer

description	;DNA (5'-D(TPTPGPAPCPTPCPTPCPTPTPAPAPGPAPGPAPGPTPCP*A)-3') ;
formula_weight	6437.2 kDa
src_method	syn
entity_count	2

Polymer

type	polydeoxyribonucleotide
strand_id	C,D
atom_count	3726

TTGACTCTCTTAAGAGAGTCA

Entity 2 polymer

description	INTRON-ENCODED HOMING ENDONUCLEASE I-PPOI
formula_weight	17680.0 kDa
ec_number	3.1.-.-
src_method	man
entity_count	2

Polymer

type	polypeptide(L)
strand_id	A,B

ALTNAQILAVIDSWEETVGQFPVITHHVPLGGGLQGTLHCYEIPLAAPYGVGFAKNGPTRWQYKRTINQVVHRWGSHTVPFLLEPDNINGKTCTASHLCHNTRCHNPLHLCWESLDDNKGRNWCPGPNGGCVHAVVCLRQGPLYGPGATVAGPQQRGSHFVV

Source

organism	Physarum polycephalum
ncbi_taxonomy_id	5791
host_organism	Escherichia coli

Entity 3 non-polymer

description	SODIUM ION
formula_weight	23.0 kDa
src_method	syn
entity_count	2

Entity 4 non-polymer

description	ZINC ION
formula_weight	65.4 kDa
src_method	syn
entity_count	4

Entity 5 water

description	water
formula_weight	18.0 kDa
src_method	nat
entity_count	376

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6. Citations (1)

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length_a	113.866 Å
length_b	113.866 Å
length_c	89.040 Å
angle_alpha	90.00 °
angle_beta	90.00 °
angle_gamma	120.00 °
z_pdb	12

space_group	P 31 2 1
int_tables_number	152
crystal_system	—

resolution	2.100 Å
resolution_low	50.000 Å
r_factor	0.1910
r_work	0.1910
r_free	0.2160

fit	/app/data/saxs_computation/cz/1cz0/1cz0.fit
pddf	/app/data/pr_computation/cz/1cz0/1cz0.pr.out
profile	/app/data/saxs_computation/cz/1cz0/1cz0.dat

INTRON ENCODED HOMING ENDONUCLEASE I-PPOI/DNA COMPLEX LACKING CATALYTIC METAL ION

SAXS Profile

P(r) Distribution

1. Structure Basics

Download Data

2. SAXS Parameters (CRYSOL)

3. P(r) Analysis (GNOM)

4. Crystallography & Experiment

Unit Cell

Symmetry

Refinement

Diffraction

5. Entities & Polymer Info (5)

Entity 1 polymer

Polymer

Entity 2 polymer

Polymer

Source

Entity 3 non-polymer

Entity 4 non-polymer

Entity 5 water

6. Citations (1)

7. Files & Curves (10)

Files (7)

Curves (3)