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1evw

L116A MUTANT OF THE HOMING ENDONUCLEASE I-PPOI COMPLEXED TO HOMING SITE DNA.

Method: X-RAY DIFFRACTION
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1. Protein Identity and Related Structures Protein Identity & Related Structures

I-PPOI HOMING ENDONUCLEASE

Physarum polycephalum

UniProt Q94702

State in the Current Structure

Assembly Physical composition Protein state Molecular copy count Associated components Data consistency
1 Protein–DNA Homooligomer Protein 2 DNA 4 ;DNA (5'-D(*TP*GP*GP*CP*TP*AP*CP*CP*TP*TP*AP*A)-3') ; × 1 ;DNA (5'-D(P*GP*AP*GP*AP*GP*TP*CP*A)-3') ; × 1 ;DNA (5'-D(*TP*GP*AP*CP*TP*CP*TP*CP*TP*TP*AP*A)-3') ; × 1 ;DNA (5'-D(P*GP*GP*TP*AP*GP*CP*CP*A)-3') ; × 1 MAGNESIUM ION × 2 ZINC ION × 4 Consistent with all polymers
2 Protein–DNA Homooligomer Protein 2 DNA 4 ;DNA (5'-D(*TP*GP*GP*CP*TP*AP*CP*CP*TP*TP*AP*A)-3') ; × 1 ;DNA (5'-D(P*GP*AP*GP*AP*GP*TP*CP*A)-3') ; × 1 ;DNA (5'-D(*TP*GP*AP*CP*TP*CP*TP*CP*TP*TP*AP*A)-3') ; × 1 ;DNA (5'-D(P*GP*GP*TP*AP*GP*CP*CP*A)-3') ; × 1 MAGNESIUM ION × 2 ZINC ION × 4 Consistent with all polymers

Other States of the Same Protein in the Database

Monomer

None

Homodimer
1evx

1 assemblies

Homotetramer

None

Other homooligomers

None

Heteromeric or nucleic-acid complex
1a73 1a74 1cyq 1cz0 1evw 1evw 1ipp 2o6m 8vmo 8vmp 8vmq 8vmr

50 assemblies; showing the first 12

View Construct and Data Evidence
UniProt name PPO1_PHYPO
Isoform —
PDB entities 5
Chains and sequence ranges Author chain A； PDBConstruct 1–163； UniProt 1–163 Author chain B； PDBConstruct 1–163； UniProt 1–163 Author chain C； PDBConstruct 1–163； UniProt 1–163 Author chain D； PDBConstruct 1–163； UniProt 1–163

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

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2. Structure Basics 2. Structure Basics

Entry ID entry_id1evw
Deposition date deposition_date2000-04-20
Structure title titleL116A MUTANT OF THE HOMING ENDONUCLEASE I-PPOI COMPLEXED TO HOMING SITE DNA.
Keywords keywordsDNA binding B-sheets; C-terminal exchanged dimer interface; bent DNA, Hydrolase-DNA COMPLEX; Hydrolase/DNA
Experimental Method methodX-RAY DIFFRACTION
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3. Official assembly/model SAXS Official SAXS Profiles

This page reads only the latest assembly calculations. Every curve maps to an explicit PDB entry, official biological assembly and coordinate model.

1evw__assembly_1__model_1

Assembly 1 · Model 1 · CRYSOL 4.1.3-1-20251215 (887e7ef)

Download this curve (.dat)

1evw__assembly_1__model_1 | I(q)

10-2 10-1 106 107 q (1/Angstrom) I(q)
100 plotted points; both axes use logarithmic scales.

1evw__assembly_1__model_1 | P(r) · Pending

The new assembly/model P(r) has not been calculated yet This placeholder does not display legacy data r (Angstrom) P(r)
P(r) will be calculated and displayed separately for the same assembly/model.
Rg（Guinier）25.18 Å
Rg (electron density)24.99 Å
Total Rg25.56 Å
Atom count3306
Residues364
Excluded volume55666 ų
Maximum q0.500 Å⁻¹
Assembly Model Structure unit Oligomeric description Status CRYSOL Actions
1 1 1evw__assembly_1__model_1 hexameric （6） Success 4.1.3-1-20251215 (887e7ef) View Download
2 1 1evw__assembly_2__model_1 hexameric （6） Success 4.1.3-1-20251215 (887e7ef) View Download
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4. Crystallography and Experiment 4. Crystallography & Experiment

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5. Entities and Polymers Entities & Polymers (7)

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6. Fold Classification (SCOP + CATH) 8 domains

SCOP 2.08 (4 domains)

Domain ID domain_idd1evwa_
Class classd — Alpha and beta proteins (a+b)
Fold Fold foldd.4 — His-Me finger endonucleases
Superfamily Superfamily superfamilyd.4.1 — His-Me finger endonucleases
Family Family familyd.4.1.3 — Intron-encoded homing endonucleases
Domain ID domain_idd1evwb_
Class classd — Alpha and beta proteins (a+b)
Fold Fold foldd.4 — His-Me finger endonucleases
Superfamily Superfamily superfamilyd.4.1 — His-Me finger endonucleases
Family Family familyd.4.1.3 — Intron-encoded homing endonucleases
Domain ID domain_idd1evwc_
Class classd — Alpha and beta proteins (a+b)
Fold Fold foldd.4 — His-Me finger endonucleases
Superfamily Superfamily superfamilyd.4.1 — His-Me finger endonucleases
Family Family familyd.4.1.3 — Intron-encoded homing endonucleases
Domain ID domain_idd1evwd_
Class classd — Alpha and beta proteins (a+b)
Fold Fold foldd.4 — His-Me finger endonucleases
Superfamily Superfamily superfamilyd.4.1 — His-Me finger endonucleases
Family Family familyd.4.1.3 — Intron-encoded homing endonucleases

CATH v4.4 (4 domains)

Domain ID domain_id1evwA00
Class class3 — Alpha Beta
Architecture architecture90 — Alpha-Beta Complex
Topology topology75 — Homing Intron 3 (I-Ppo) Encoded Endonuclease; Chain A
Homologous superfamily homologous superfamily10 — Homing Intron 3 (I-ppo) Encoded Endonuclease; Chain A
Domain ID domain_id1evwB00
Class class3 — Alpha Beta
Architecture architecture90 — Alpha-Beta Complex
Topology topology75 — Homing Intron 3 (I-Ppo) Encoded Endonuclease; Chain A
Homologous superfamily homologous superfamily10 — Homing Intron 3 (I-ppo) Encoded Endonuclease; Chain A
Domain ID domain_id1evwC00
Class class3 — Alpha Beta
Architecture architecture90 — Alpha-Beta Complex
Topology topology75 — Homing Intron 3 (I-Ppo) Encoded Endonuclease; Chain A
Homologous superfamily homologous superfamily10 — Homing Intron 3 (I-ppo) Encoded Endonuclease; Chain A
Domain ID domain_id1evwD00
Class class3 — Alpha Beta
Architecture architecture90 — Alpha-Beta Complex
Topology topology75 — Homing Intron 3 (I-Ppo) Encoded Endonuclease; Chain A
Homologous superfamily homologous superfamily10 — Homing Intron 3 (I-ppo) Encoded Endonuclease; Chain A
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7. Citations (1)