L116A MUTANT OF THE HOMING ENDONUCLEASE I-PPOI COMPLEXED TO HOMING SITE DNA.

entry_id	1evw
deposition_date	2000-04-20
title	L116A MUTANT OF THE HOMING ENDONUCLEASE I-PPOI COMPLEXED TO HOMING SITE DNA.
keywords	DNA binding B-sheets; C-terminal exchanged dimer interface; bent DNA, Hydrolase-DNA COMPLEX; Hydrolase/DNA
method	X-RAY DIFFRACTION

rg_guinier	37.27 Å
rg_electron	37.84 Å
i0	202593000.00
molecular_weight	95699.0 kDa
excluded_volume	111330 ų
envelope_volume	161070 ų
shell_volume	37901 ų
envelope_diameter	132.5 Å
shell_rg	40.80 Å
envelope_rg	36.95 Å
shape_rg	37.86 Å
total_rg	37.96 Å
total_atoms	6612
n_residues	728
n_harmonics	20
q_range	— – 0.5000 Å−1
n_points	101
shell_type	directional
solvent_density	0.3340 e/ų
contrast_shell	0.0300 e/ų
crysol_version	4.1.3

dmax	129.6 Å
rg_real	37.54 Å
rg_real_error	1.52 Å
i0_real	2.0260e+08
i0_real_error	4.1490e+06
rg_reciprocal	37.38 Å
i0_reciprocal	202600000.0000
total_estimate	0.8544
solution_quality	GOOD a GOOD solution
n_peaks	1
r_peak_primary	46.3 Å
skewness	0.439
kurtosis	-0.364
angular_range	— – 0.2100 Å−1
current_alpha	0.0000
highest_alpha	12260000.0000
n_real_points	43
gnom_version	4.1.3
quality_criteria	AN1: 0.000; Oscil: 0.805; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.857; Smooth: 0.830

Unit Cell

length_a	182.000 Å
length_b	73.100 Å
length_c	92.700 Å
angle_alpha	90.00 °
angle_beta	95.40 °
angle_gamma	90.00 °
z_pdb	16

Symmetry

space_group	C 1 2 1
int_tables_number	5
crystal_system	—

Refinement

resolution	3.100 Å
resolution_low	50.000 Å
r_factor	—
r_work	0.2730
r_free	0.3200

Diffraction

temperature

77.0 K

Files (7)

• pr_distribution /app/data/pr_computation/ev/1evw/pr_distribution.json
• pr_output /app/data/pr_computation/ev/1evw/1evw.pr.out
• pr_plot /app/data/visualization/pr_computation/ev/1evw/1evw_pr_distribution.png
• saxs_curve_dat /app/data/saxs_computation/ev/1evw/1evw.dat
• saxs_fit /app/data/saxs_computation/ev/1evw/1evw.fit
• saxs_log /app/data/saxs_computation/ev/1evw/1evw.log
• saxs_plot /app/data/visualization/saxs_computation/ev/1evw/1evw_saxs_profile.png

Curves (3)

fit	/app/data/saxs_computation/ev/1evw/1evw.fit
pddf	/app/data/pr_computation/ev/1evw/1evw.pr.out
profile	/app/data/saxs_computation/ev/1evw/1evw.dat