SAXSdb — 1azs

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1. Structure Basics

entry_id	1azs
deposition_date	1997-11-20
title	COMPLEX OF GS-ALPHA WITH THE CATALYTIC DOMAINS OF MAMMALIAN ADENYLYL CYCLASE
keywords	COMPLEX (LYASE-HYDROLASE), HYDROLASE, SIGNAL TRANSDUCING PROTEIN, CYCLASE, EFFECTOR ENZYME, COMPLEX (LYASE-HYDROLASE) complex; COMPLEX (LYASE/HYDROLASE)
method	X-RAY DIFFRACTION

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Download Data

SAXS curve (.dat) P(r) distribution (.pr.out) Structure (CIF)

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2. SAXS Parameters (CRYSOL)

rg_guinier	32.08 Å
rg_electron	31.38 Å
i0	110368000.00
molecular_weight	82826.0 kDa
excluded_volume	103400 Å³
envelope_volume	132670 Å³
shell_volume	35307 Å³
envelope_diameter	99.8 Å
shell_rg	38.20 Å
envelope_rg	31.16 Å
shape_rg	31.38 Å
total_rg	31.98 Å
total_atoms	5812
n_residues	718
n_harmonics	20
q_range	— – 0.5000 Å⁻¹
n_points	101
shell_type	directional
solvent_density	0.3340 e/Å³
contrast_shell	0.0300 e/Å³
crysol_version	4.1.3

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3. P(r) Analysis (GNOM)

dmax	102.0 Å
rg_real	32.08 Å
rg_real_error	0.76 Å
i0_real	1.1040e+08
i0_real_error	1.7760e+06
rg_reciprocal	32.08 Å
i0_reciprocal	110400000.0000
total_estimate	0.8309
solution_quality	GOOD a GOOD solution
n_peaks	1
r_peak_primary	31.2 Å
skewness	0.255
kurtosis	-0.678
angular_range	— – 0.2450 Å⁻¹
current_alpha	0.0000
highest_alpha	22940000.0000
n_real_points	50
gnom_version	4.1.3
quality_criteria	AN1: 0.000; Oscil: 0.940; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.976; Smooth: 0.000

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4. Crystallography & Experiment

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5. Entities & Polymer Info (7)

Entity 1 polymer

description	VC1
formula_weight	25010.8 kDa
ec_number	4.6.1.1
mutation	V476M, N-TERMINAL HEXAHISTIDINE TAG
src_method	man
entity_count	1

Polymer

type	polypeptide(L)
strand_id	A
atom_count	5881

MHHHHHHAMEMKADINAKQEDMMFHKIYIQKHDNVSILFADIEGFTSLASQCTAQELVMTLNELFARFDKLAAENHCLRIKILGDCYYCVSGLPEARADHAHCCVEMGMDMIEAISLVREMTGVNVNMRVGIHSGRVHCGVLGLRKWQFDVWSNDVTLANHMEAGGKAGRIHITKATLSYLNGDYEVEPGCGGERNAYLKEHSIETFLILRCTQKRKEEK

Source

organism	Canis lupus familiaris
common_name	dog
ncbi_taxonomy_id	9615
host_organism	Escherichia coli BL21(DE3)

Entity 2 polymer

description	IIC2
formula_weight	23717.0 kDa
ec_number	4.6.1.1
src_method	man
entity_count	1

Polymer

type	polypeptide(L)
strand_id	B

RSLKNEELYHQSYDCVCVMFASIPDFKEFYTESDVNKEGLECLRLLNEIIADFDDLLSKPKFSGVEKIKTIGSTYMAATGLSAIPSQEHAQEPERQYMHIGTMVEFAYALVGKLDAINKHSFNDFKLRVGINHGPVIAGVIGAQKPQYDIWGNTVNVASRMDSTGVLDKIQVTEETSLILQTLGYTCTCRGIINVKGKGDLKTYFVNTEMSR

Source

organism	Rattus norvegicus
common_name	Norway rat
ncbi_taxonomy_id	10116
host_organism	Escherichia coli BL21(DE3)

Entity 3 polymer

description	GS-ALPHA
formula_weight	46712.5 kDa
mutation	C-TERMINAL HEXAHISTIDINE TAG, NOT PALMITOYLATED AT AMINO TERMINUS
src_method	man
entity_count	1

Polymer

type	polypeptide(L)
strand_id	C

MGCLGNSKTEDQRNEEKAQREANKKIEKQLQKDKQVYRATHRLLLLGAGESGKSTIVKQMRILHVNGFNGEGGEEDPQAARSNSDGEKATKVQDIKNNLKEAIETIVAAMSNLVPPVELANPENQFRVDYILSVMNVPDFDFPPEFYEHAKALWEDEGVRACYERSNEYQLIDCAQYFLDKIDVIKQDDYVPSDQDLLRCRVLTSGIFETKFQVDKVNFHMFDVGGQRDERRKWIQCFNDVTAIIFVVASSSYNMVIREDNQTNRLQEALNLFKSIWNNRWLRTISVILFLNKQDLLAEKVLAGKSKIEDYFPEFARYTTPEDATPEPGEDPRVTRAKYFIRDEFLRISTASGDGRHYCYPHFTCAVDTENIRRVFNDCRDIIQRMHLRQYELLGGHHHHHH

Source

organism	Bos taurus
common_name	cattle
ncbi_taxonomy_id	9913
host_organism	Escherichia coli BL21(DE3)

Entity 4 non-polymer

description	METHYLPIPERAZINOFORSKOLIN
formula_weight	550.7 kDa
src_method	syn
entity_count	1

Entity 5 non-polymer

description	MAGNESIUM ION
formula_weight	24.3 kDa
src_method	syn
entity_count	1

Entity 6 non-polymer

description	5'-GUANOSINE-DIPHOSPHATE-MONOTHIOPHOSPHATE
formula_weight	539.2 kDa
src_method	syn
entity_count	1

Entity 7 water

description	water
formula_weight	18.0 kDa
src_method	nat
entity_count	69

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6. Citations (1)

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length_a	119.000 Å
length_b	134.050 Å
length_c	70.650 Å
angle_alpha	90.00 °
angle_beta	90.00 °
angle_gamma	90.00 °
z_pdb	4

space_group	P 21 21 2
int_tables_number	18
crystal_system	—

resolution	2.300 Å
resolution_low	15.000 Å
r_factor	0.2190
r_work	0.2190
r_free	0.2820

fit	/app/data/saxs_computation/az/1azs/1azs.fit
pddf	/app/data/pr_computation/az/1azs/1azs.pr.out
profile	/app/data/saxs_computation/az/1azs/1azs.dat

COMPLEX OF GS-ALPHA WITH THE CATALYTIC DOMAINS OF MAMMALIAN ADENYLYL CYCLASE

SAXS Profile

P(r) Distribution

1. Structure Basics

Download Data

2. SAXS Parameters (CRYSOL)

3. P(r) Analysis (GNOM)

4. Crystallography & Experiment

Unit Cell

Symmetry

Refinement

Diffraction

5. Entities & Polymer Info (7)

Entity 1 polymer

Polymer

Source

Entity 2 polymer

Polymer

Source

Entity 3 polymer

Polymer

Source

Entity 4 non-polymer

Entity 5 non-polymer

Entity 6 non-polymer

Entity 7 water

6. Citations (1)

7. Files & Curves (10)

Files (7)

Curves (3)