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1cs4

;COMPLEX OF GS-ALPHA WITH THE CATALYTIC DOMAINS OF MAMMALIAN ADENYLYL CYCLASE: COMPLEX WITH 2'-DEOXY-ADENOSINE 3'-MONOPHOSPHATE, PYROPHOSPHATE AND MG ;

Method: X-RAY DIFFRACTION D_max: 102.5 Å Quality: GOOD

SAXS Profile

P(r) Distribution

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1. Structure Basics

entry_id	1cs4
deposition_date	1999-08-16
title	;COMPLEX OF GS-ALPHA WITH THE CATALYTIC DOMAINS OF MAMMALIAN ADENYLYL CYCLASE: COMPLEX WITH 2'-DEOXY-ADENOSINE 3'-MONOPHOSPHATE, PYROPHOSPHATE AND MG ;
keywords	;COMPLEX (LYASE-HYDROLASE), HYDROLASE, SIGNAL TRANSDUCING PROTEIN, CYCLASE, EFFECTOR ENZYME, LYASE-HYDROLASE COMPLEX, LYASE-LYASE-SIGNALING PROTEIN COMPLEX ;; LYASE/LYASE/SIGNALING PROTEIN
method	X-RAY DIFFRACTION

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Download Data

SAXS curve (.dat) P(r) distribution (.pr.out) Structure (CIF)

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2. SAXS Parameters (CRYSOL)

rg_guinier	32.05 Å
rg_electron	31.40 Å
i0	110937000.00
molecular_weight	82332.0 kDa
excluded_volume	102490 Å³
envelope_volume	132430 Å³
shell_volume	35262 Å³
envelope_diameter	101.1 Å
shell_rg	38.18 Å
envelope_rg	31.20 Å
shape_rg	31.41 Å
total_rg	31.97 Å
total_atoms	5766
n_residues	708
n_harmonics	20
q_range	— – 0.5000 Å⁻¹
n_points	101
shell_type	directional
solvent_density	0.3340 e/Å³
contrast_shell	0.0300 e/Å³
crysol_version	4.1.3

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3. P(r) Analysis (GNOM)

dmax	102.5 Å
rg_real	32.07 Å
rg_real_error	0.61 Å
i0_real	1.1090e+08
i0_real_error	1.4380e+06
rg_reciprocal	32.07 Å
i0_reciprocal	110900000.0000
total_estimate	0.8968
solution_quality	GOOD a GOOD solution
n_peaks	2
r_peak_primary	29.3 Å
skewness	0.256
kurtosis	-0.710
angular_range	— – 0.2450 Å⁻¹
current_alpha	0.0000
highest_alpha	31360000.0000
n_real_points	50
gnom_version	4.1.3
quality_criteria	AN1: 0.000; Oscil: 0.920; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.965; Smooth: 0.929

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4. Crystallography & Experiment

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5. Entities & Polymer Info (11)

Entity 1 polymer

description	TYPE V ADENYLATE CYCLASE
formula_weight	25526.5 kDa
ec_number	4.6.1.1
mutation	V476M
src_method	man
entity_count	1

Polymer

type	polypeptide(L)
strand_id	A
atom_count	5843

MHHHHHHAMEMKADINAKQEDMMFHKIYIQKHDNVSILFADIEGFTSLASQCTAQELVMTLNELFARFDKLAAENHCLRIKILGDCYYCVSGLPEARADHAHCCVEMGMDMIEAISLVREMTGVNVNMRVGIHSGRVHCGVLGLRKWQFDVWSNDVTLANHMEAGGKAGRIHITKATLSYLNGDYEVEPGCGGERNAYLKEHSIETFLILRCTQKRKEEKAMIAK

Source

organism	Canis lupus familiaris
common_name	dog
ncbi_taxonomy_id	9615
host_organism	Escherichia coli

Entity 2 polymer

description	TYPE II ADENYLATE CYCLASE
formula_weight	23717.0 kDa
ec_number	4.6.1.1
src_method	man
entity_count	1

Polymer

type	polypeptide(L)
strand_id	B

RSLKNEELYHQSYDCVCVMFASIPDFKEFYTESDVNKEGLECLRLLNEIIADFDDLLSKPKFSGVEKIKTIGSTYMAATGLSAIPSQEHAQEPERQYMHIGTMVEFAYALVGKLDAINKHSFNDFKLRVGINHGPVIAGVIGAQKPQYDIWGNTVNVASRMDSTGVLDKIQVTEETSLILQTLGYTCTCRGIINVKGKGDLKTYFVNTEMSR

Source

organism	Rattus norvegicus
common_name	Norway rat
ncbi_taxonomy_id	10116
host_organism	Escherichia coli

Entity 3 polymer

description	GUANINE NUCLEOTIDE-BINDING PROTEIN G(S)
formula_weight	45769.5 kDa
mutation	TRYPSINIZED, SHORT FORM
src_method	man
entity_count	1

Polymer

type	polypeptide(L)
strand_id	C

MGCLGNSKTEDQRNEEKAQREANKKIEKQLQKDKQVYRATHRLLLLGAGESGKSTIVKQMRILHVNGFNGEGGEEDPQAARSNSDGEKATKVQDIKNNLKEAIETIVAAMSNLVPPVELANPENQFRVDYILSVMNVPDFDFPPEFYEHAKALWEDEGVRACYERSNEYQLIDCAQYFLDKIDVIKQDDYVPSDQDLLRCRVLTSGIFETKFQVDKVNFHMFDVGGQRDERRKWIQCFNDVTAIIFVVASSSYNMVIREDNQTNRLQEALNLFKSIWNNRWLRTISVILFLNKQDLLAEKVLAGKSKIEDYFPEFARYTTPEDATPEPGEDPRVTRAKYFIRDEFLRISTASGDGRHYCYPHFTCAVDTENIRRVFNDCRDIIQRMHLRQYELL

Source

organism	Bos taurus
common_name	cattle
ncbi_taxonomy_id	9913
host_organism	Escherichia coli

Entity 4 non-polymer

description	MAGNESIUM ION
formula_weight	24.3 kDa
src_method	syn
entity_count	2

Entity 5 non-polymer

description	FORSKOLIN
formula_weight	410.5 kDa
src_method	syn
entity_count	1

Entity 6 non-polymer

description	2-(N-MORPHOLINO)-ETHANESULFONIC ACID
formula_weight	195.2 kDa
src_method	syn
entity_count	2

Entity 7 non-polymer

description	PYROPHOSPHATE 2-
formula_weight	176.0 kDa
src_method	syn
entity_count	1

Entity 8 non-polymer

description	;2'-DEOXY-ADENOSINE 3'-MONOPHOSPHATE ;
formula_weight	331.2 kDa
src_method	syn
entity_count	1

Entity 9 non-polymer

description	CHLORIDE ION
formula_weight	35.5 kDa
src_method	syn
entity_count	1

Entity 10 non-polymer

description	5'-GUANOSINE-DIPHOSPHATE-MONOTHIOPHOSPHATE
formula_weight	539.2 kDa
src_method	syn
entity_count	1

Entity 11 water

description	water
formula_weight	18.0 kDa
src_method	nat
entity_count	77

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6. Citations (2)

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length_a	118.500 Å
length_b	134.500 Å
length_c	71.300 Å
angle_alpha	90.00 °
angle_beta	90.00 °
angle_gamma	90.00 °
z_pdb	4

space_group	P 21 21 2
int_tables_number	18
crystal_system	—

resolution	2.500 Å
resolution_low	15.000 Å
r_factor	0.2210
r_work	0.2160
r_free	0.2640

fit	/app/data/saxs_computation/cs/1cs4/1cs4.fit
pddf	/app/data/pr_computation/cs/1cs4/1cs4.pr.out
profile	/app/data/saxs_computation/cs/1cs4/1cs4.dat

;COMPLEX OF GS-ALPHA WITH THE CATALYTIC DOMAINS OF MAMMALIAN ADENYLYL CYCLASE: COMPLEX WITH 2'-DEOXY-ADENOSINE 3'-MONOPHOSPHATE, PYROPHOSPHATE AND MG ;

SAXS Profile

P(r) Distribution

1. Structure Basics

Download Data

2. SAXS Parameters (CRYSOL)

3. P(r) Analysis (GNOM)

4. Crystallography & Experiment

Unit Cell

Symmetry

Refinement

Diffraction

5. Entities & Polymer Info (11)

Entity 1 polymer

Polymer

Source

Entity 2 polymer

Polymer

Source

Entity 3 polymer

Polymer

Source

Entity 4 non-polymer

Entity 5 non-polymer

Entity 6 non-polymer

Entity 7 non-polymer

Entity 8 non-polymer

Entity 9 non-polymer

Entity 10 non-polymer

Entity 11 water

6. Citations (2)

7. Files & Curves (10)

Files (7)

Curves (3)