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1cjt

;COMPLEX OF GS-ALPHA WITH THE CATALYTIC DOMAINS OF MAMMALIAN ADENYLYL CYCLASE: COMPLEX WITH BETA-L-2',3'-DIDEOXYATP, MN, AND MG ;

Method: X-RAY DIFFRACTION D_max: 102.2 Å Quality: GOOD

SAXS Profile

P(r) Distribution

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1. Structure Basics

entry_id	1cjt
deposition_date	1999-04-16
title	;COMPLEX OF GS-ALPHA WITH THE CATALYTIC DOMAINS OF MAMMALIAN ADENYLYL CYCLASE: COMPLEX WITH BETA-L-2',3'-DIDEOXYATP, MN, AND MG ;
keywords	COMPLEX (LYASE-HYDROLASE), HYDROLASE, SIGNAL TRANSDUCING PROTEIN, CYCLASE, EFFECTOR ENZYME, LYASE-LYASE-SIGNALING PROTEIN COMPLEX; LYASE/LYASE/SIGNALING PROTEIN
method	X-RAY DIFFRACTION

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Download Data

SAXS curve (.dat) P(r) distribution (.pr.out) Structure (CIF)

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2. SAXS Parameters (CRYSOL)

rg_guinier	31.97 Å
rg_electron	31.29 Å
i0	110548000.00
molecular_weight	82183.0 kDa
excluded_volume	102270 Å³
envelope_volume	131700 Å³
shell_volume	35185 Å³
envelope_diameter	104.6 Å
shell_rg	38.19 Å
envelope_rg	31.10 Å
shape_rg	31.30 Å
total_rg	31.87 Å
total_atoms	5755
n_residues	708
n_harmonics	20
q_range	— – 0.5000 Å⁻¹
n_points	101
shell_type	directional
solvent_density	0.3340 e/Å³
contrast_shell	0.0300 e/Å³
crysol_version	4.1.3

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3. P(r) Analysis (GNOM)

dmax	102.2 Å
rg_real	31.98 Å
rg_real_error	1.13 Å
i0_real	1.1050e+08
i0_real_error	1.7360e+06
rg_reciprocal	31.98 Å
i0_reciprocal	110500000.0000
total_estimate	0.8977
solution_quality	GOOD a GOOD solution
n_peaks	2
r_peak_primary	30.7 Å
skewness	0.254
kurtosis	-0.720
angular_range	— – 0.2500 Å⁻¹
current_alpha	0.0000
highest_alpha	30920000.0000
n_real_points	51
gnom_version	4.1.3
quality_criteria	AN1: 0.000; Oscil: 0.916; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.970; Smooth: 0.949

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4. Crystallography & Experiment

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5. Entities & Polymer Info (11)

Entity 1 polymer

description	ADENYLATE CYCLASE, TYPE V
formula_weight	24495.4 kDa
ec_number	4.6.1.1
mutation	V476M
src_method	man
entity_count	1

Polymer

type	polypeptide(L)
strand_id	A
atom_count	5821

MEMKADINAKQEDMMFHKIYIQKHDNVSILFADIEGFTSLASQCTAQELVMTLNELFARFDKLAAENHCLRIKILGDCYYCVSGLPEARADHAHCCVEMGMDMIEAISLVREMTGVNVNMRVGIHSGRVHCGVLGLRKWQFDVWSNDVTLANHMEAGGKAGRIHITKATLSYLNGDYEVEPGCGGERNAYLKEHSIETFLILRCTQKRKEEKAMIAK

Source

organism	Canis lupus familiaris
common_name	dog
ncbi_taxonomy_id	9615
host_organism	Escherichia coli BL21(DE3)

Entity 2 polymer

description	ADENYLATE CYCLASE, TYPE II
formula_weight	23717.0 kDa
ec_number	4.6.1.1
src_method	man
entity_count	1

Polymer

type	polypeptide(L)
strand_id	B

RSLKNEELYHQSYDCVCVMFASIPDFKEFYTESDVNKEGLECLRLLNEIIADFDDLLSKPKFSGVEKIKTIGSTYMAATGLSAIPSQEHAQEPERQYMHIGTMVEFAYALVGKLDAINKHSFNDFKLRVGINHGPVIAGVIGAQKPQYDIWGNTVNVASRMDSTGVLDKIQVTEETSLILQTLGYTCTCRGIINVKGKGDLKTYFVNTEMSR

Source

organism	Rattus norvegicus
common_name	Norway rat
ncbi_taxonomy_id	10116
host_organism	Escherichia coli BL21(DE3)

Entity 3 polymer

description	GUANINE NUCLEOTIDE-BINDING PROTEIN G(S)
formula_weight	46656.4 kDa
mutation	N-TERMINAL HEXAHISTIDINE TAG
src_method	man
entity_count	1

Polymer

type	polypeptide(L)
strand_id	C

MGCLGNSKTEDQRNEEKAQREANKKIEKQLQKDKQVYRATHRLLLLGAGESGKSTIVKQMRILHVNGFNGGEGGEEDPNAKSNSDGEKATKVQDIKNNLKEAIETIVAAMSNLVPPVELANPENQFRVDYILSVMNVPDFDFPPEFYEHAKALWEDEGVRACYERSNEYQLIDCAQYFLDKIDVIKQDDYVPSDQDLLRCRVLTSGIFETKFQVDKVNFHMFDVGGQRDERRKWIQCFNDVTAIIFVVASSSYNMVIREDNQTNRLQEALNLFKSIWNNRWLRTISVILFLNKQDLLAEKVLAGKSKIEDYFPEFARYTTPEDATPEPGEDPRVTRAKYFIRDEFLRISTASGDGRHYCYPHFTCAVDTENIRRVFNDCRDIIQRMHLRQYELLGGHHHHHH

Source

organism	Bos taurus
common_name	cattle
ncbi_taxonomy_id	9913
host_organism	Escherichia coli BL21(DE3)

Entity 4 non-polymer

description	MAGNESIUM ION
formula_weight	24.3 kDa
src_method	syn
entity_count	2

Entity 5 non-polymer

description	MANGANESE (II) ION
formula_weight	54.9 kDa
src_method	syn
entity_count	1

Entity 6 non-polymer

description	FORSKOLIN
formula_weight	410.5 kDa
src_method	syn
entity_count	1

Entity 7 non-polymer

description	2',3'-DIDEOXYADENOSINE-5'-TRIPHOSPHATE
formula_weight	475.2 kDa
src_method	syn
entity_count	1

Entity 8 non-polymer

description	2-(N-MORPHOLINO)-ETHANESULFONIC ACID
formula_weight	195.2 kDa
src_method	syn
entity_count	1

Entity 9 non-polymer

description	CHLORIDE ION
formula_weight	35.5 kDa
src_method	syn
entity_count	1

Entity 10 non-polymer

description	5'-GUANOSINE-DIPHOSPHATE-MONOTHIOPHOSPHATE
formula_weight	539.2 kDa
src_method	syn
entity_count	1

Entity 11 water

description	water
formula_weight	18.0 kDa
src_method	nat
entity_count	66

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6. Citations (2)

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length_a	118.200 Å
length_b	134.200 Å
length_c	71.300 Å
angle_alpha	90.00 °
angle_beta	90.00 °
angle_gamma	90.00 °
z_pdb	4

space_group	P 21 21 2
int_tables_number	18
crystal_system	—

resolution	2.800 Å
resolution_low	15.000 Å
r_factor	—
r_work	0.2060
r_free	0.2520

fit	/app/data/saxs_computation/cj/1cjt/1cjt.fit
pddf	/app/data/pr_computation/cj/1cjt/1cjt.pr.out
profile	/app/data/saxs_computation/cj/1cjt/1cjt.dat

;COMPLEX OF GS-ALPHA WITH THE CATALYTIC DOMAINS OF MAMMALIAN ADENYLYL CYCLASE: COMPLEX WITH BETA-L-2',3'-DIDEOXYATP, MN, AND MG ;

SAXS Profile

P(r) Distribution

1. Structure Basics

Download Data

2. SAXS Parameters (CRYSOL)

3. P(r) Analysis (GNOM)

4. Crystallography & Experiment

Unit Cell

Symmetry

Refinement

Diffraction

5. Entities & Polymer Info (11)

Entity 1 polymer

Polymer

Source

Entity 2 polymer

Polymer

Source

Entity 3 polymer

Polymer

Source

Entity 4 non-polymer

Entity 5 non-polymer

Entity 6 non-polymer

Entity 7 non-polymer

Entity 8 non-polymer

Entity 9 non-polymer

Entity 10 non-polymer

Entity 11 water

6. Citations (2)

7. Files & Curves (10)

Files (7)

Curves (3)