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1cjk

;COMPLEX OF GS-ALPHA WITH THE CATALYTIC DOMAINS OF MAMMALIAN ADENYLYL CYCLASE: COMPLEX WITH ADENOSINE 5'-(ALPHA THIO)-TRIPHOSPHATE (RP), MG, AND MN ;

Method: X-RAY DIFFRACTION D_max: 102.4 Å Quality: GOOD

SAXS Profile

P(r) Distribution

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1. Structure Basics

entry_id	1cjk
deposition_date	1999-04-16
title	;COMPLEX OF GS-ALPHA WITH THE CATALYTIC DOMAINS OF MAMMALIAN ADENYLYL CYCLASE: COMPLEX WITH ADENOSINE 5'-(ALPHA THIO)-TRIPHOSPHATE (RP), MG, AND MN ;
keywords	COMPLEX (LYASE-HYDROLASE), HYDROLASE, SIGNAL TRANSDUCING PROTEIN, CYCLASE, EFFECTOR ENZYME, LYASE-LYASE-SIGNALING PROTEIN COMPLEX; LYASE/LYASE/SIGNALING PROTEIN
method	X-RAY DIFFRACTION

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Download Data

SAXS curve (.dat) P(r) distribution (.pr.out) Structure (CIF)

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2. SAXS Parameters (CRYSOL)

rg_guinier	32.22 Å
rg_electron	31.56 Å
i0	111037000.00
molecular_weight	82345.0 kDa
excluded_volume	102470 Å³
envelope_volume	133670 Å³
shell_volume	35498 Å³
envelope_diameter	102.7 Å
shell_rg	38.32 Å
envelope_rg	31.28 Å
shape_rg	31.57 Å
total_rg	32.12 Å
total_atoms	5765
n_residues	709
n_harmonics	20
q_range	— – 0.5000 Å⁻¹
n_points	101
shell_type	directional
solvent_density	0.3340 e/Å³
contrast_shell	0.0300 e/Å³
crysol_version	4.1.3

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3. P(r) Analysis (GNOM)

dmax	102.4 Å
rg_real	32.24 Å
rg_real_error	0.58 Å
i0_real	1.1100e+08
i0_real_error	1.6470e+06
rg_reciprocal	32.24 Å
i0_reciprocal	111000000.0000
total_estimate	0.8960
solution_quality	GOOD a GOOD solution
n_peaks	2
r_peak_primary	29.3 Å
skewness	0.254
kurtosis	-0.719
angular_range	— – 0.2450 Å⁻¹
current_alpha	0.0000
highest_alpha	31530000.0000
n_real_points	50
gnom_version	4.1.3
quality_criteria	AN1: 0.000; Oscil: 0.923; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.966; Smooth: 0.909

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4. Crystallography & Experiment

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5. Entities & Polymer Info (11)

Entity 1 polymer

description	ADENYLATE CYCLASE, TYPE V
formula_weight	24495.4 kDa
ec_number	4.6.1.1
mutation	V476M
src_method	man
entity_count	1

Polymer

type	polypeptide(L)
strand_id	A
atom_count	5799

MEMKADINAKQEDMMFHKIYIQKHDNVSILFADIEGFTSLASQCTAQELVMTLNELFARFDKLAAENHCLRIKILGDCYYCVSGLPEARADHAHCCVEMGMDMIEAISLVREMTGVNVNMRVGIHSGRVHCGVLGLRKWQFDVWSNDVTLANHMEAGGKAGRIHITKATLSYLNGDYEVEPGCGGERNAYLKEHSIETFLILRCTQKRKEEKAMIAK

Source

organism	Canis lupus familiaris
common_name	dog
ncbi_taxonomy_id	9615
host_organism	Escherichia coli BL21(DE3)

Entity 2 polymer

description	ADENYLATE CYCLASE, TYPE II
formula_weight	23717.0 kDa
ec_number	4.6.1.1
src_method	man
entity_count	1

Polymer

type	polypeptide(L)
strand_id	B

RSLKNEELYHQSYDCVCVMFASIPDFKEFYTESDVNKEGLECLRLLNEIIADFDDLLSKPKFSGVEKIKTIGSTYMAATGLSAIPSQEHAQEPERQYMHIGTMVEFAYALVGKLDAINKHSFNDFKLRVGINHGPVIAGVIGAQKPQYDIWGNTVNVASRMDSTGVLDKIQVTEETSLILQTLGYTCTCRGIINVKGKGDLKTYFVNTEMSR

Source

organism	Rattus norvegicus
common_name	Norway rat
ncbi_taxonomy_id	10116
host_organism	Escherichia coli BL21(DE3)

Entity 3 polymer

description	GUANINE NUCLEOTIDE-BINDING PROTEIN G(S)
formula_weight	46656.4 kDa
mutation	N-TERMINAL HEXAHISTIDINE TAG
src_method	man
entity_count	1

Polymer

type	polypeptide(L)
strand_id	C

MGCLGNSKTEDQRNEEKAQREANKKIEKQLQKDKQVYRATHRLLLLGAGESGKSTIVKQMRILHVNGFNGGEGGEEDPNAKSNSDGEKATKVQDIKNNLKEAIETIVAAMSNLVPPVELANPENQFRVDYILSVMNVPDFDFPPEFYEHAKALWEDEGVRACYERSNEYQLIDCAQYFLDKIDVIKQDDYVPSDQDLLRCRVLTSGIFETKFQVDKVNFHMFDVGGQRDERRKWIQCFNDVTAIIFVVASSSYNMVIREDNQTNRLQEALNLFKSIWNNRWLRTISVILFLNKQDLLAEKVLAGKSKIEDYFPEFARYTTPEDATPEPGEDPRVTRAKYFIRDEFLRISTASGDGRHYCYPHFTCAVDTENIRRVFNDCRDIIQRMHLRQYELLGGHHHHHH

Source

organism	Bos taurus
common_name	cattle
ncbi_taxonomy_id	9913
host_organism	Escherichia coli BL21(DE3)

Entity 4 non-polymer

description	MAGNESIUM ION
formula_weight	24.3 kDa
src_method	syn
entity_count	2

Entity 5 non-polymer

description	MANGANESE (II) ION
formula_weight	54.9 kDa
src_method	syn
entity_count	1

Entity 6 non-polymer

description	FORSKOLIN
formula_weight	410.5 kDa
src_method	syn
entity_count	1

Entity 7 non-polymer

description	ADENOSINE-5'-RP-ALPHA-THIO-TRIPHOSPHATE
formula_weight	523.2 kDa
src_method	syn
entity_count	1

Entity 8 non-polymer

description	2-(N-MORPHOLINO)-ETHANESULFONIC ACID
formula_weight	195.2 kDa
src_method	syn
entity_count	1

Entity 9 non-polymer

description	CHLORIDE ION
formula_weight	35.5 kDa
src_method	syn
entity_count	1

Entity 10 non-polymer

description	5'-GUANOSINE-DIPHOSPHATE-MONOTHIOPHOSPHATE
formula_weight	539.2 kDa
src_method	syn
entity_count	1

Entity 11 water

description	water
formula_weight	18.0 kDa
src_method	nat
entity_count	34

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6. Citations (2)

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length_a	118.900 Å
length_b	134.900 Å
length_c	72.200 Å
angle_alpha	90.00 °
angle_beta	90.00 °
angle_gamma	90.00 °
z_pdb	4

space_group	P 21 21 2
int_tables_number	18
crystal_system	—

resolution	3.000 Å
resolution_low	15.000 Å
r_factor	—
r_work	0.2200
r_free	0.2660

fit	/app/data/saxs_computation/cj/1cjk/1cjk.fit
pddf	/app/data/pr_computation/cj/1cjk/1cjk.pr.out
profile	/app/data/saxs_computation/cj/1cjk/1cjk.dat

;COMPLEX OF GS-ALPHA WITH THE CATALYTIC DOMAINS OF MAMMALIAN ADENYLYL CYCLASE: COMPLEX WITH ADENOSINE 5'-(ALPHA THIO)-TRIPHOSPHATE (RP), MG, AND MN ;

SAXS Profile

P(r) Distribution

1. Structure Basics

Download Data

2. SAXS Parameters (CRYSOL)

3. P(r) Analysis (GNOM)

4. Crystallography & Experiment

Unit Cell

Symmetry

Refinement

Diffraction

5. Entities & Polymer Info (11)

Entity 1 polymer

Polymer

Source

Entity 2 polymer

Polymer

Source

Entity 3 polymer

Polymer

Source

Entity 4 non-polymer

Entity 5 non-polymer

Entity 6 non-polymer

Entity 7 non-polymer

Entity 8 non-polymer

Entity 9 non-polymer

Entity 10 non-polymer

Entity 11 water

6. Citations (2)

7. Files & Curves (10)

Files (7)

Curves (3)