SAXSdb — 1bkd

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1. Structure Basics

entry_id	1bkd
deposition_date	1998-07-06
title	COMPLEX OF HUMAN H-RAS WITH HUMAN SOS-1
keywords	COMPLEX (ONCOGENE PROTEIN-EXCHANGE FACTOR), SMALL GTPASE, EXCHANGE FACTOR, COMPLEX(ONCOGENE PROTEIN-EXCHANGE FACTOR) COMPLEX; COMPLEX(ONCOGENE PROTEIN/EXCHANGE FACTOR)
method	X-RAY DIFFRACTION

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Download Data

SAXS curve (.dat) P(r) distribution (.pr.out) Structure (CIF)

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2. SAXS Parameters (CRYSOL)

rg_guinier	29.08 Å
rg_electron	28.40 Å
i0	78794500.00
molecular_weight	70673.0 kDa
excluded_volume	89011 Å³
envelope_volume	112400 Å³
shell_volume	33510 Å³
envelope_diameter	106.2 Å
shell_rg	35.07 Å
envelope_rg	28.69 Å
shape_rg	28.39 Å
total_rg	29.12 Å
total_atoms	4984
n_residues	605
n_harmonics	20
q_range	— – 0.5000 Å⁻¹
n_points	101
shell_type	directional
solvent_density	0.3340 e/Å³
contrast_shell	0.0300 e/Å³
crysol_version	4.1.3

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3. P(r) Analysis (GNOM)

dmax	100.2 Å
rg_real	29.09 Å
rg_real_error	0.65 Å
i0_real	7.8790e+07
i0_real_error	1.1190e+06
rg_reciprocal	29.09 Å
i0_reciprocal	78790000.0000
total_estimate	0.8743
solution_quality	GOOD a GOOD solution
n_peaks	1
r_peak_primary	31.0 Å
skewness	0.356
kurtosis	-0.369
angular_range	— – 0.2750 Å⁻¹
current_alpha	0.0001
highest_alpha	25610000.0000
n_real_points	56
gnom_version	4.1.3
quality_criteria	AN1: 0.000; Oscil: 0.815; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.923; Smooth: 0.994

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4. Crystallography & Experiment

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5. Entities & Polymer Info (3)

Entity 1 polymer

description	H-RAS
formula_weight	18875.2 kDa
src_method	man
entity_count	1

Polymer

type	polypeptide(L)
strand_id	R
atom_count	5010

MTEYKLVVVGAGGVGKSALTIQLIQNHFVDEYDPTIEDSYRKQVVIDGETCLLDILDTAGQEEYSAMRDQYMRTGEGFLCVFAINNTKSFEDIHQYREQIKRVKDSDDVPMVLVGNKCDLAARTVESRQAQDLARSYGIPYIETSAKTRQGVEDAFYTLVREIRQH

Source

organism	Homo sapiens
common_name	human
ncbi_taxonomy_id	9606
host_organism	Escherichia coli BL21(DE3)

Entity 2 polymer

description	SON OF SEVENLESS-1
formula_weight	56019.1 kDa
src_method	man
entity_count	1

Polymer

type	polypeptide(L)
strand_id	S

RLPSADVYRFAEPDSEENIIFEENMQPKAGIPIIKAGTVIKLIERLTYHMYADPNFVRTFLTTYRSFCKPQELLSLIIERFEIPEPEPTEADRIAIENGDQPLSAELKRFRKEYIQPVQLRVLNVCRHWVEHHFYDFERDAYLLQRMEEFIGTVRGKAMKKWVESITKIIQRKKIARDNGPGHNITFQSSPPTVEWHISRPGHIETFDLLTLHPIEIARQLTLLESDLYRAVQPSELVGSVWTKEDKEINSPNLLKMIRHTTNLTLWFEKCIVETENLEERVAVVSRIIEILQVFQELNNFNGVLEVVSAMNSSPVYRLDHTFEQIPSRQKKILEEAHELSEDHYKKYLAKLRSINPPCVPFFGIYLTNILKTEEGNPEVLKRHGKELINFSKRRKVAEITGEIQQYQNQPYCLRVESDIKRFFENLNPMGNSMEKEFTDYLFNKSLEIEPRNPKPLPRFPKKYSYPLKSPGVRPSN

Source

organism	Homo sapiens
common_name	human
ncbi_taxonomy_id	9606
host_organism	Escherichia coli BL21(DE3)

Entity 3 water

description	water
formula_weight	18.0 kDa
src_method	nat
entity_count	26

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6. Citations (1)

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length_a	142.700 Å
length_b	142.700 Å
length_c	207.900 Å
angle_alpha	90.00 °
angle_beta	90.00 °
angle_gamma	90.00 °
z_pdb	16

space_group	I 4 2 2
int_tables_number	97
crystal_system	—

resolution	2.800 Å
resolution_low	30.000 Å
r_factor	0.2220
r_work	0.2220
r_free	0.2810

fit	/app/data/saxs_computation/bk/1bkd/1bkd.fit
pddf	/app/data/pr_computation/bk/1bkd/1bkd.pr.out
profile	/app/data/saxs_computation/bk/1bkd/1bkd.dat

COMPLEX OF HUMAN H-RAS WITH HUMAN SOS-1

SAXS Profile

P(r) Distribution

1. Structure Basics

Download Data

2. SAXS Parameters (CRYSOL)

3. P(r) Analysis (GNOM)

4. Crystallography & Experiment

Unit Cell

Symmetry

Refinement

Diffraction

5. Entities & Polymer Info (3)

Entity 1 polymer

Polymer

Source

Entity 2 polymer

Polymer

Source

Entity 3 water

6. Citations (1)

7. Files & Curves (10)

Files (7)

Curves (3)