1qnt

X-ray structure of human O6alkylguanine-DNA alkyltransferase

Method: X-RAY DIFFRACTION Dmax: 52.8 Å Quality: GOOD

SAXS 散射曲线 SAXS Profile

SAXS profile for 1qnt

P(r) 距离分布 P(r) Distribution

P(r) distribution for 1qnt
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1. 结构基本信息 1. Structure Basics

条目编号 entry_id1qnt
沉积日期 deposition_date1999-10-20
结构标题 titleX-ray structure of human O6alkylguanine-DNA alkyltransferase
关键词 keywordsDNA REPAIR, ALKYLTRANSFERASE, METHYLTRANSFERASE; DNA REPAIR
实验方法 methodX-RAY DIFFRACTION

2. SAXS 参数 (CRYSOL 理论计算) 2. SAXS Parameters (CRYSOL)

回转半径 Rg (Guinier) rg_guinier16.86
回转半径 Rg (电子) rg_electron15.50
零角强度 I(0) i05895650.00
分子量 molecular_weight17947.0 kDa
排除体积 excluded_volume22695 ų
包络体积 envelope_volume26073 ų
水化壳体积 shell_volume14273 ų
包络直径 envelope_diameter53.0
壳层 Rg shell_rg21.23
包络 Rg envelope_rg15.70
形状 Rg shape_rg15.47
总 Rg total_rg16.68
总原子数 total_atoms1264
残基数 n_residues166
球谐函数阶数 n_harmonics20
q 范围 q_range— – 0.5000 −1
数据点数 n_points101
壳层类型 shell_typedirectional
溶剂电子密度 solvent_density0.3340 e/ų
壳层衬度 contrast_shell0.0300 e/ų
CRYSOL 版本 crysol_version4.1.3

3. P(r) 距离分布 (GNOM 反演) 3. P(r) Analysis (GNOM)

最大尺寸 Dmax dmax52.8
Rg (实空间) rg_real16.75
Rg 误差 (实空间) rg_real_error0.29
I(0) (实空间) i0_real5.8960e+06
I(0) 误差 (实空间) i0_real_error7.5170e+04
Rg (倒空间) rg_reciprocal16.77
I(0) (倒空间) i0_reciprocal5896000.0000
解质量估计 total_estimate0.8978
解质量评级 solution_quality GOOD a GOOD solution
P(r) 峰数 n_peaks2
主峰位置 r_peak_primary21.6
偏度 Skewness skewness0.114
峰度 Kurtosis kurtosis-0.446
角度范围 angular_range— – 0.4700 −1
当前正则化参数 α current_alpha0.0000
最高正则化参数 α highest_alpha1232000.0000
实空间数据点数 n_real_points77
GNOM 版本 gnom_version4.1.3
质量判据 quality_criteria AN1: 0.000; Oscil: 0.901; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.993; Smooth: 0.969

4. 晶体学与实验 4. Crystallography & Experiment

5. 实体与聚合体信息 (2)

6. 折叠分类 (SCOP + CATH) 4 domains

SCOP 2.08 (2 domains)

结构域编号 domain_idd1qnta1
类 Class classa — All alpha proteins
折叠类型 Fold folda.4 — DNA/RNA-binding 3-helical bundle
超家族 Superfamily superfamilya.4.2 — Methylated DNA-protein cysteine methyltransferase, C-terminal domain
家族 Family familya.4.2.1 — Methylated DNA-protein cysteine methyltransferase, C-terminal domain
结构域编号 domain_idd1qnta2
类 Class classc — Alpha and beta proteins (a/b)
折叠类型 Fold foldc.55 — Ribonuclease H-like motif
超家族 Superfamily superfamilyc.55.7 — Methylated DNA-protein cysteine methyltransferase domain
家族 Family familyc.55.7.1 — Methylated DNA-protein cysteine methyltransferase domain

CATH v4.4 (2 domains)

结构域编号 domain_id1qntA01
类 Class class3 — Alpha Beta
架构 Architecture architecture30 — 2-Layer Sandwich
拓扑 Topology topology160 — Double Stranded RNA Binding Domain
同源超家族 H-superfamily homologous superfamily70 — Methylated DNA-protein cysteine methyltransferase domain
结构域编号 domain_id1qntA02
类 Class class1 — Mainly Alpha
架构 Architecture architecture10 — Orthogonal Bundle
拓扑 Topology topology10 — Arc Repressor Mutant, subunit A
同源超家族 H-superfamily homologous superfamily10 — Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain

7. 引用文献 (3)

8. 文件与曲线 (10)