2kz7
Solution structure of the CARMIL CAH3a/b domain bound to capping protein (CP)
SAXS 散射曲线 SAXS Profile
P(r) 距离分布 P(r) Distribution
1. 结构基本信息 1. Structure Basics
| 条目编号 entry_id | 2kz7 |
| 沉积日期 deposition_date | 2010-06-12 |
| 结构标题 title | Solution structure of the CARMIL CAH3a/b domain bound to capping protein (CP) |
| 关键词 keywords | TROSY, Paramagnetic relaxation enhancement, protein-protein complex, PROTEIN BINDING; PROTEIN BINDING |
| 实验方法 method | SOLUTION NMR |
2. SAXS 参数 (CRYSOL 理论计算) 2. SAXS Parameters (CRYSOL)
| 回转半径 Rg (Guinier) rg_guinier | 28.59 Å |
| 回转半径 Rg (电子) rg_electron | 28.22 Å |
| 零角强度 I(0) i0 | 7411320000.00 |
| 分子量 molecular_weight | 713140.0 kDa |
| 排除体积 excluded_volume | 886070 ų |
| 包络体积 envelope_volume | 238660 ų |
| 水化壳体积 shell_volume | 57933 ų |
| 包络直径 envelope_diameter | 106.6 Å |
| 壳层 Rg shell_rg | 42.05 Å |
| 包络 Rg envelope_rg | 32.27 Å |
| 形状 Rg shape_rg | 28.20 Å |
| 总 Rg total_rg | 28.51 Å |
| 总原子数 total_atoms | 99730 |
| 残基数 n_residues | 6270 |
| 球谐函数阶数 n_harmonics | 20 |
| q 范围 q_range | — – 0.5000 Å−1 |
| 数据点数 n_points | 101 |
| 壳层类型 shell_type | directional |
| 溶剂电子密度 solvent_density | 0.3340 e/ų |
| 壳层衬度 contrast_shell | 0.0300 e/ų |
| CRYSOL 版本 crysol_version | 4.1.3 |
3. P(r) 距离分布 (GNOM 反演) 3. P(r) Analysis (GNOM)
| 最大尺寸 Dmax dmax | 92.7 Å |
| Rg (实空间) rg_real | 28.56 Å |
| Rg 误差 (实空间) rg_real_error | 0.62 Å |
| I(0) (实空间) i0_real | 7.4110e+09 |
| I(0) 误差 (实空间) i0_real_error | 1.0400e+08 |
| Rg (倒空间) rg_reciprocal | 28.58 Å |
| I(0) (倒空间) i0_reciprocal | 7411000000.0000 |
| 解质量估计 total_estimate | 0.8927 |
| 解质量评级 solution_quality | GOOD a GOOD solution |
| P(r) 峰数 n_peaks | 2 |
| 主峰位置 r_peak_primary | 32.0 Å |
| 偏度 Skewness skewness | 0.341 |
| 峰度 Kurtosis kurtosis | -0.383 |
| 角度范围 angular_range | — – 0.2750 Å−1 |
| 当前正则化参数 α current_alpha | 0.0000 |
| 最高正则化参数 α highest_alpha | 89250000.0000 |
| 实空间数据点数 n_real_points | 56 |
| GNOM 版本 gnom_version | 4.1.3 |
| 质量判据 quality_criteria | AN1: 0.000; Oscil: 0.878; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.993; Smooth: 0.973 |
4. 晶体学与实验 4. Crystallography & Experiment
5. 实体与聚合体信息 (3)
6. 折叠分类 (SCOP + CATH) 6 domains
SCOP 2.08 (2 domains)
| 结构域编号 domain_id | d2kz7a_ |
| 类 Class class | e — Multi-domain proteins (alpha and beta) |
| 折叠类型 Fold fold | e.43 — Subunits of heterodimeric actin filament capping protein Capz |
| 超家族 Superfamily superfamily | e.43.1 — Subunits of heterodimeric actin filament capping protein Capz |
| 家族 Family family | e.43.1.1 — Capz alpha-1 subunit |
| 结构域编号 domain_id | d2kz7b_ |
| 类 Class class | e — Multi-domain proteins (alpha and beta) |
| 折叠类型 Fold fold | e.43 — Subunits of heterodimeric actin filament capping protein Capz |
| 超家族 Superfamily superfamily | e.43.1 — Subunits of heterodimeric actin filament capping protein Capz |
| 家族 Family family | e.43.1.2 — Capz beta-1 subunit |
CATH v4.4 (4 domains)
| 结构域编号 domain_id | 2kz7A01 |
| 类 Class class | 3 — Alpha Beta |
| 架构 Architecture architecture | 30 — 2-Layer Sandwich |
| 拓扑 Topology topology | 1140 — Ribosomal protein S3 C-terminal domain |
| 同源超家族 H-superfamily homologous superfamily | 60 — F-actin capping protein, alpha subunit |
| 结构域编号 domain_id | 2kz7A02 |
| 类 Class class | 3 — Alpha Beta |
| 架构 Architecture architecture | 90 — Alpha-Beta Complex |
| 拓扑 Topology topology | 1150 — Aspartate Aminotransferase, domain 1 |
| 同源超家族 H-superfamily homologous superfamily | 210 — F-actin capping protein, beta subunit |
| 结构域编号 domain_id | 2kz7B01 |
| 类 Class class | 1 — Mainly Alpha |
| 架构 Architecture architecture | 20 — Up-down Bundle |
| 拓扑 Topology topology | 58 — Methane Monooxygenase Hydroxylase; Chain G, domain 1 |
| 同源超家族 H-superfamily homologous superfamily | 570 — F-actin capping protein, alpha/beta subunit, N-terminal domain |
| 结构域编号 domain_id | 2kz7B02 |
| 类 Class class | 3 — Alpha Beta |
| 架构 Architecture architecture | 90 — Alpha-Beta Complex |
| 拓扑 Topology topology | 1150 — Aspartate Aminotransferase, domain 1 |
| 同源超家族 H-superfamily homologous superfamily | 210 — F-actin capping protein, beta subunit |