2kxp
Solution NMR structure of V-1 bound to capping protein (CP)
SAXS 散射曲线 SAXS Profile
P(r) 距离分布 P(r) Distribution
1. 结构基本信息 1. Structure Basics
| 条目编号 entry_id | 2kxp |
| 沉积日期 deposition_date | 2010-05-11 |
| 结构标题 title | Solution NMR structure of V-1 bound to capping protein (CP) |
| 关键词 keywords | protein-protein interaction, capping protein, V-1, PROTEIN BINDING; PROTEIN BINDING |
| 实验方法 method | SOLUTION NMR |
2. SAXS 参数 (CRYSOL 理论计算) 2. SAXS Parameters (CRYSOL)
| 回转半径 Rg (Guinier) rg_guinier | 29.69 Å |
| 回转半径 Rg (电子) rg_electron | 29.30 Å |
| 零角强度 I(0) i0 | 8030570000.00 |
| 分子量 molecular_weight | 750260.0 kDa |
| 排除体积 excluded_volume | 934950 ų |
| 包络体积 envelope_volume | 227360 ų |
| 水化壳体积 shell_volume | 55295 ų |
| 包络直径 envelope_diameter | 104.1 Å |
| 壳层 Rg shell_rg | 41.73 Å |
| 包络 Rg envelope_rg | 32.37 Å |
| 形状 Rg shape_rg | 29.27 Å |
| 总 Rg total_rg | 29.58 Å |
| 总原子数 total_atoms | 105110 |
| 残基数 n_residues | 6630 |
| 球谐函数阶数 n_harmonics | 20 |
| q 范围 q_range | — – 0.5000 Å−1 |
| 数据点数 n_points | 101 |
| 壳层类型 shell_type | directional |
| 溶剂电子密度 solvent_density | 0.3340 e/ų |
| 壳层衬度 contrast_shell | 0.0300 e/ų |
| CRYSOL 版本 crysol_version | 4.1.3 |
3. P(r) 距离分布 (GNOM 反演) 3. P(r) Analysis (GNOM)
| 最大尺寸 Dmax dmax | 91.2 Å |
| Rg (实空间) rg_real | 29.63 Å |
| Rg 误差 (实空间) rg_real_error | 0.60 Å |
| I(0) (实空间) i0_real | 8.0310e+09 |
| I(0) 误差 (实空间) i0_real_error | 1.1670e+08 |
| Rg (倒空间) rg_reciprocal | 29.66 Å |
| I(0) (倒空间) i0_reciprocal | 8031000000.0000 |
| 解质量估计 total_estimate | 0.9064 |
| 解质量评级 solution_quality | EXCELLENT a EXCELLENT solution |
| P(r) 峰数 n_peaks | 2 |
| 主峰位置 r_peak_primary | 34.4 Å |
| 偏度 Skewness skewness | 0.244 |
| 峰度 Kurtosis kurtosis | -0.556 |
| 角度范围 angular_range | — – 0.2650 Å−1 |
| 当前正则化参数 α current_alpha | 0.0000 |
| 最高正则化参数 α highest_alpha | 39470000.0000 |
| 实空间数据点数 n_real_points | 54 |
| GNOM 版本 gnom_version | 4.1.3 |
| 质量判据 quality_criteria | AN1: 0.000; Oscil: 0.966; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 1.000; Smooth: 0.880 |
4. 晶体学与实验 4. Crystallography & Experiment
5. 实体与聚合体信息 (3)
6. 折叠分类 (SCOP + CATH) 9 domains
SCOP 2.08 (3 domains)
| 结构域编号 domain_id | d2kxpa_ |
| 类 Class class | e — Multi-domain proteins (alpha and beta) |
| 折叠类型 Fold fold | e.43 — Subunits of heterodimeric actin filament capping protein Capz |
| 超家族 Superfamily superfamily | e.43.1 — Subunits of heterodimeric actin filament capping protein Capz |
| 家族 Family family | e.43.1.1 — Capz alpha-1 subunit |
| 结构域编号 domain_id | d2kxpb_ |
| 类 Class class | e — Multi-domain proteins (alpha and beta) |
| 折叠类型 Fold fold | e.43 — Subunits of heterodimeric actin filament capping protein Capz |
| 超家族 Superfamily superfamily | e.43.1 — Subunits of heterodimeric actin filament capping protein Capz |
| 家族 Family family | e.43.1.2 — Capz beta-1 subunit |
| 结构域编号 domain_id | d2kxpc_ |
| 类 Class class | d — Alpha and beta proteins (a+b) |
| 折叠类型 Fold fold | d.211 — beta-hairpin-alpha-hairpin repeat |
| 超家族 Superfamily superfamily | d.211.1 — Ankyrin repeat |
| 家族 Family family | d.211.1.1 — Ankyrin repeat |
CATH v4.4 (6 domains)
| 结构域编号 domain_id | 2kxpA01 |
| 类 Class class | 3 — Alpha Beta |
| 架构 Architecture architecture | 30 — 2-Layer Sandwich |
| 拓扑 Topology topology | 1140 — Ribosomal protein S3 C-terminal domain |
| 同源超家族 H-superfamily homologous superfamily | 60 — F-actin capping protein, alpha subunit |
| 结构域编号 domain_id | 2kxpA02 |
| 类 Class class | 3 — Alpha Beta |
| 架构 Architecture architecture | 90 — Alpha-Beta Complex |
| 拓扑 Topology topology | 1150 — Aspartate Aminotransferase, domain 1 |
| 同源超家族 H-superfamily homologous superfamily | 210 — F-actin capping protein, beta subunit |
| 结构域编号 domain_id | 2kxpB01 |
| 类 Class class | 1 — Mainly Alpha |
| 架构 Architecture architecture | 20 — Up-down Bundle |
| 拓扑 Topology topology | 58 — Methane Monooxygenase Hydroxylase; Chain G, domain 1 |
| 同源超家族 H-superfamily homologous superfamily | 570 — F-actin capping protein, alpha/beta subunit, N-terminal domain |
| 结构域编号 domain_id | 2kxpB02 |
| 类 Class class | 3 — Alpha Beta |
| 架构 Architecture architecture | 90 — Alpha-Beta Complex |
| 拓扑 Topology topology | 1150 — Aspartate Aminotransferase, domain 1 |
| 同源超家族 H-superfamily homologous superfamily | 210 — F-actin capping protein, beta subunit |
| 结构域编号 domain_id | 2kxpB03 |
| 类 Class class | 6 — Special |
| 架构 Architecture architecture | 10 — Helix non-globular |
| 拓扑 Topology topology | 250 — Single alpha-helices involved in coiled-coils or other helix-helix interfaces |
| 同源超家族 H-superfamily homologous superfamily | 30 — Capz alpha-1 subunit |
| 结构域编号 domain_id | 2kxpC00 |
| 类 Class class | 1 — Mainly Alpha |
| 架构 Architecture architecture | 25 — Alpha Horseshoe |
| 拓扑 Topology topology | 40 — Serine Threonine Protein Phosphatase 5, Tetratricopeptide repeat |
| 同源超家族 H-superfamily homologous superfamily | 20 — Ankyrin repeat-containing domain |